LOC100123217 cDNA ORF clone, Nasonia vitripennis(jewel wasp)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100123217
Entrez Gene ID	100123217
Full Name	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Gene Type	protein-coding
Organism	Nasonia vitripennis(jewel wasp)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003427203.3	XP_003427251.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
XM_003427203.4	XP_003427251.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

Pathways from BioSystems
Gene Ontology

The following LOC100123217 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100123217 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONd14417	XM_003427203.4 Latest version!	Nasonia vitripennis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100123217), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
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ONd14417	XM_003427203.3	Nasonia vitripennis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100123217), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100123217 ( XM_003427203.4 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_003427251.1 Nasonia vitripennis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100123217), mRNA.

Price & Availability↑

CloneID	ONd14417
Clone ID Related Accession (Same CDS sequence)	XM_003427203.3 , XM_003427203.4
Accession Version	XM_003427203.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Nasonia vitripennis(jewel wasp)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022279647.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 24, 2019 this sequence version replaced XM_003427203.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nasonia vitripennis Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAA TAATTCCTGA AATGGAGCAG ATCATCAGTC AGCTTGAAAG AGGGACTGTG 
GTCACCAAGT TCTTTCCAAG AAAAAGACCA GAAAAGAAGA CATTAATGAT ACGCAGAGAA 
ACCAGACAAA TAGTTTGGGC AAGAAGCACC ACATCAAGAC CTTTTGATGG CTCTGTGGAT 
ATTCGAGAAG TGAAAGAAAT AAGAATTGGA AAGAACTCAA AAGACTTTGA GAAATGGCCA 
GAAGATGCTA AACGAATTGA TAATCTACGT TGTTTTGTAG TTTACTATGG CTCTGAGTTC 
AGACTAAAAA CTCTTTCAAT ATCAGCTCTG AGTGAAAAAG AGTGTGAACT CTGGATTAAA 
GGACTTAGAC ACTTGATGCA AGATACTATA AATGCTCCAT ACCCATTGCA AGTTGAAAGA 
TGGCTAAGAA AAGAATTTTA TGCAATGGAA AATTCCAGAG AAATGGTTAC TTTAAAAGAT 
GTCAAAGCCT TTTTACCAAG AGTAAACTGT AAGATAGCAA CTAACAAACT CAGAGAAGTT 
TTTCAAGAAG TCGATACAAA AAATAGAACT GAAATAGGAT TTGACGATTT TGTGATACTT 
TACCATAAAC TTATGTTTGA TCCAAACGTG AGCAATTTAA CATATTGGAC AAGACTGTTA 
GTTTATTCTA ACTCTGGGAT GTTGGTGACA CTACAAGAAT TTCAAAATTT TCTACTCACA 
GAACAACAAG ATCCTTCAGG AAATATTGAA TCAGACGTTT CGCTTTTTAT TCGCGAGTAT 
CTGCAAGATC CTCAAAGAGA CGTTCAGGAA CCTTTTTTTC ATTTCTCTGA ATTCATAGAT 
TTTTTGTTTT CAAAACAGAA TGAAATTTGG GATAGTCAAT ATGATCATGT CTATCACGAT 
ATGACTAGGC CTATGTCTCA TTATTGGATT GCTTCATCGC ATAATACTTA CTTGATGGGT 
GATCAAATCA GTAGTGAGAG CAGTTGCCAA GCATATGTTA GAGCTCTGCG AGTTGGTTGC 
AGGTGTATAG AACTAGATTG CTGGGATGGA CCAGATGGGA TGCCTTTTAT ATTTCATGGA 
CATACTTTGA CGACGAAAAT CAAATTTTTA GACGTAATTA AAACAATTAA AGAACACGCT 
TTTGTCACTT CCGAATATCC TGTGATTCTC TCAATAGAAG ACAACTGCAC ACTACCGCAA 
CAGCGGAAAA TGGCTGTGGC TATGCAGGAA GTCTTTGGCG ACATGTTGCT GGTTCAACCC 
GTCGACAAGA GTGAATCCAC TTTACCCTCG CCCTACGCTC TGCGAAGAAA AATTCTACTC 
AAGCACAAAA AGCTTCCCGA CGGCGTTGAC GAAAGTTCCT TTGTCATTCG AAACGATGAA 
AGCCGTCAAG AGATGGATTT GAGAAACACT ATTAAAAATG GTATATTGTA TCTGGAGGAT 
CCAGTAGATC GAGAATGGAA TCCACACTTT TTTGTCCTGA CGCAGCAGAA ACTCTTTTAT 
ACAGATACTT TCTCAAAACC GCAAGAAACC GAACCGGACG ACGATGACGA AGCCGTTGTT 
AGAACTCCCG GTGATGGTGT TCCAAACGAT GAATTACATT TTGGAGAAAT GTGGTTCCAT 
GGTAAATTGG CCAGAGGAAG AGAGGAAGCT GAAGAACTTT TACGAAGGTA TTCCAATTTA 
GGAGATGGAA CGTTTTTGGT CAGGCAATGC GTGACCTTTG TGGGAGACTA TTGCTTATCA 
TTTTGGAGGA AAGGAAAAGT CAATCATTGC CGTATCAAGT TAAAACAAGA CATGGGTCAG 
ACACAGTTTT ATTTGATAGA CACTCACTGT TTTGACAGTT TATACAGTCT CATCACTTAT 
TATCGTAATA ATCCTCTGAG AAGCCAGGAA TTTTTAATCA CATTACAAGA ACCTGTACCG 
CAGCCAAGTA AACACGAAGA AAAAGAATGG TGGCACTCTG AATGCTCAAG AACATTGGCC 
GAGGAAATGT TGAAGCGTGT ACCAACCGAT GGAGCGTTTT TAGTGAGGCC AAGCGAAAAG 
GAGAATAATT GTTACGCCAT TTCCTTCAGG GCTGAAAATA AAATAAAGCA TTGCCGCATA 
AAACTTGAAG GACGGCTATA TGCAATCGGA ACAGTGCAAT TTGAAAGTTT GGTGGAATTA 
GTCAATTACT ACGAACGCCA TCCTTTGTAT AAAAAAATTA AGTTAAGTTT TCCAGTTAAT 
CAAGAAATAG TCCGGCAAAG AGGTTTGGAT GTTGACGACG GTTCCGTATA TGGAATTCCG 
GGCTATATGG ATCCAACAAC TTTTGTTTCA AAAATCACAG TCAAAGCAAT GTACGATTAC 
AAGGCTCGTC GAGACGACGA ATTAACTTTA GTAAAACATG CTATAATTAC GAATGTAAAT 
AGACAACAAA ACGGTGGTTG GTGGAGAGGT GACTATGGTG GTAAGAAGCA GCACTGGTTC 
CCGGCCAATT ACGTTGAAGA AATCGAGCAA GATAATCAGA CGGATTCATC AGATTCGATG 
ATGCTGGGAA ATTTGCAAAA AGGCTCTTTA GACATAATAG GCGCTGTTGT GGAGTTAGCC 
ATGTCCGACC GACCTGGTTT AGAATGGATT TTACGGATAC AGAATCCAAG CATGTGCTCT 
GTTTTCGAGG CAGCAGCTCC ATCCAAAGAT ATCGCCGTCG AATGGGTACA AAAAATTCAC 
GAGACTGGAC AAAACGCCAG CGTCAGGGAG AATCAACACA AAGAAATGGA AAGAGCTTGG 
AGGATAGCAA AAGAAATGTC CAATCTCATA GTTTACTTTA GATCAGTAGC CTTCAACGTG 
GAGCGGCTAC GAGCCAAGGG ATTTATTTTC AACGAAATGA GCAGCTTTCC GGAGACTAAA 
GCAGAAAAGC TTATGTGCCA ACAAGAAAAC AAATTCTTTT TACGTTATCA CCAGGTGCAA 
TTCAGCCGAG TCTATCCCAA GGGCCAACGA ATAGATTCTT CAAATTACAA TCCGGTACCT 
ATGTGGAACT CGGGTTGTCA GATGGTGGCC CTAAATTACC AAACAGGCGA TAAGCCAATG 
CAGCTAAATC AGGCGAAATT TCGAGAGAAC GGTAACTGCG GTTATCTGTT GAAGCCTGAT 
TTTATGTTTC ACGAAGATTT CACTCCGTAC GATAAGAGTT CATTGACCGG AGTGGACACG 
TTAAAGATTA CGCTGCACGT CATAGGTGCA AGACACCTAA GTAAGCCGGG AAGGGGCACA 
GCGAGTCCTT TCGTACAAGT CGAAGTAGTG GGTGCAGATT TCGATAGCGG CGTTAAGTTA 
ACGACTAAGA CTATTACCGA TAACGGTTTC AATCCCATGT GGAACGAATC CTGTGAGTTC 
CTAGTAGCAA ATCCATATTT GGCTATGCTT CGGTTCGTTG TTCAAGACGA GGATATGTTT 
GGAGATAGCA ATTTTATTGG ACAGGCAACT TACCCTATTC GCTGCTTGAG GACAGGTTAC 
AGAAGTGTTG TTCTGAAAAA CAGTTATAGC GAAGACCTTG AATTGGCGTC TCTCCTAGTG 
CATGTGAAAA TTATTAGCTC AGGTGACTTG ATGCACAATA ATACTCCAAA ACACGATGGA 
CGATCCATTG CTCTGAACTC TCGAGCCGTG GACAGAGATT CACAACTTTC TGGAAGATTG 
CACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003427251.1
CDS	416..4084
Translation	MNGIIPEMEQIISQLERGTVVTKFFPRKRPEKKTLMIRRETRQIVWARSTTSRPFDGSVDIREVKEIRIGKNSKDFEKWPEDAKRIDNLRCFVVYYGSEFRLKTLSISALSEKECELWIKGLRHLMQDTINAPYPLQVERWLRKEFYAMENSREMVTLKDVKAFLPRVNCKIATNKLREVFQEVDTKNRTEIGFDDFVILYHKLMFDPNVSNLTYWTRLLVYSNSGMLVTLQEFQNFLLTEQQDPSGNIESDVSLFIREYLQDPQRDVQEPFFHFSEFIDFLFSKQNEIWDSQYDHVYHDMTRPMSHYWIASSHNTYLMGDQISSESSCQAYVRALRVGCRCIELDCWDGPDGMPFIFHGHTLTTKIKFLDVIKTIKEHAFVTSEYPVILSIEDNCTLPQQRKMAVAMQEVFGDMLLVQPVDKSESTLPSPYALRRKILLKHKKLPDGVDESSFVIRNDESRQEMDLRNTIKNGILYLEDPVDREWNPHFFVLTQQKLFYTDTFSKPQETEPDDDDEAVVRTPGDGVPNDELHFGEMWFHGKLARGREEAEELLRRYSNLGDGTFLVRQCVTFVGDYCLSFWRKGKVNHCRIKLKQDMGQTQFYLIDTHCFDSLYSLITYYRNNPLRSQEFLITLQEPVPQPSKHEEKEWWHSECSRTLAEEMLKRVPTDGAFLVRPSEKENNCYAISFRAENKIKHCRIKLEGRLYAIGTVQFESLVELVNYYERHPLYKKIKLSFPVNQEIVRQRGLDVDDGSVYGIPGYMDPTTFVSKITVKAMYDYKARRDDELTLVKHAIITNVNRQQNGGWWRGDYGGKKQHWFPANYVEEIEQDNQTDSSDSMMLGNLQKGSLDIIGAVVELAMSDRPGLEWILRIQNPSMCSVFEAAAPSKDIAVEWVQKIHETGQNASVRENQHKEMERAWRIAKEMSNLIVYFRSVAFNVERLRAKGFIFNEMSSFPETKAEKLMCQQENKFFLRYHQVQFSRVYPKGQRIDSSNYNPVPMWNSGCQMVALNYQTGDKPMQLNQAKFRENGNCGYLLKPDFMFHEDFTPYDKSSLTGVDTLKITLHVIGARHLSKPGRGTASPFVQVEVVGADFDSGVKLTTKTITDNGFNPMWNESCEFLVANPYLAMLRFVVQDEDMFGDSNFIGQATYPIRCLRTGYRSVVLKNSYSEDLELASLLVHVKIISSGDLMHNNTPKHDGRSIALNSRAVDRDSQLSGRLHA

Target ORF information:

RefSeq Version	XM_003427203.4
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100123217), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003427203.4

ORF Insert Sequence:

ATGAATGGAA TAATTCCTGA AATGGAGCAG ATCATCAGTC AGCTTGAAAG AGGGACTGTG 
GTCACCAAGT TCTTTCCAAG AAAAAGACCA GAAAAGAAGA CATTAATGAT ACGCAGAGAA 
ACCAGACAAA TAGTTTGGGC AAGAAGCACC ACATCAAGAC CTTTTGATGG CTCTGTGGAT 
ATTCGAGAAG TGAAAGAAAT AAGAATTGGA AAGAACTCAA AAGACTTTGA GAAATGGCCA 
GAAGATGCTA AACGAATTGA TAATCTACGT TGTTTTGTAG TTTACTATGG CTCTGAGTTC 
AGACTAAAAA CTCTTTCAAT ATCAGCTCTG AGTGAAAAAG AGTGTGAACT CTGGATTAAA 
GGACTTAGAC ACTTGATGCA AGATACTATA AATGCTCCAT ACCCATTGCA AGTTGAAAGA 
TGGCTAAGAA AAGAATTTTA TGCAATGGAA AATTCCAGAG AAATGGTTAC TTTAAAAGAT 
GTCAAAGCCT TTTTACCAAG AGTAAACTGT AAGATAGCAA CTAACAAACT CAGAGAAGTT 
TTTCAAGAAG TCGATACAAA AAATAGAACT GAAATAGGAT TTGACGATTT TGTGATACTT 
TACCATAAAC TTATGTTTGA TCCAAACGTG AGCAATTTAA CATATTGGAC AAGACTGTTA 
GTTTATTCTA ACTCTGGGAT GTTGGTGACA CTACAAGAAT TTCAAAATTT TCTACTCACA 
GAACAACAAG ATCCTTCAGG AAATATTGAA TCAGACGTTT CGCTTTTTAT TCGCGAGTAT 
CTGCAAGATC CTCAAAGAGA CGTTCAGGAA CCTTTTTTTC ATTTCTCTGA ATTCATAGAT 
TTTTTGTTTT CAAAACAGAA TGAAATTTGG GATAGTCAAT ATGATCATGT CTATCACGAT 
ATGACTAGGC CTATGTCTCA TTATTGGATT GCTTCATCGC ATAATACTTA CTTGATGGGT 
GATCAAATCA GTAGTGAGAG CAGTTGCCAA GCATATGTTA GAGCTCTGCG AGTTGGTTGC 
AGGTGTATAG AACTAGATTG CTGGGATGGA CCAGATGGGA TGCCTTTTAT ATTTCATGGA 
CATACTTTGA CGACGAAAAT CAAATTTTTA GACGTAATTA AAACAATTAA AGAACACGCT 
TTTGTCACTT CCGAATATCC TGTGATTCTC TCAATAGAAG ACAACTGCAC ACTACCGCAA 
CAGCGGAAAA TGGCTGTGGC TATGCAGGAA GTCTTTGGCG ACATGTTGCT GGTTCAACCC 
GTCGACAAGA GTGAATCCAC TTTACCCTCG CCCTACGCTC TGCGAAGAAA AATTCTACTC 
AAGCACAAAA AGCTTCCCGA CGGCGTTGAC GAAAGTTCCT TTGTCATTCG AAACGATGAA 
AGCCGTCAAG AGATGGATTT GAGAAACACT ATTAAAAATG GTATATTGTA TCTGGAGGAT 
CCAGTAGATC GAGAATGGAA TCCACACTTT TTTGTCCTGA CGCAGCAGAA ACTCTTTTAT 
ACAGATACTT TCTCAAAACC GCAAGAAACC GAACCGGACG ACGATGACGA AGCCGTTGTT 
AGAACTCCCG GTGATGGTGT TCCAAACGAT GAATTACATT TTGGAGAAAT GTGGTTCCAT 
GGTAAATTGG CCAGAGGAAG AGAGGAAGCT GAAGAACTTT TACGAAGGTA TTCCAATTTA 
GGAGATGGAA CGTTTTTGGT CAGGCAATGC GTGACCTTTG TGGGAGACTA TTGCTTATCA 
TTTTGGAGGA AAGGAAAAGT CAATCATTGC CGTATCAAGT TAAAACAAGA CATGGGTCAG 
ACACAGTTTT ATTTGATAGA CACTCACTGT TTTGACAGTT TATACAGTCT CATCACTTAT 
TATCGTAATA ATCCTCTGAG AAGCCAGGAA TTTTTAATCA CATTACAAGA ACCTGTACCG 
CAGCCAAGTA AACACGAAGA AAAAGAATGG TGGCACTCTG AATGCTCAAG AACATTGGCC 
GAGGAAATGT TGAAGCGTGT ACCAACCGAT GGAGCGTTTT TAGTGAGGCC AAGCGAAAAG 
GAGAATAATT GTTACGCCAT TTCCTTCAGG GCTGAAAATA AAATAAAGCA TTGCCGCATA 
AAACTTGAAG GACGGCTATA TGCAATCGGA ACAGTGCAAT TTGAAAGTTT GGTGGAATTA 
GTCAATTACT ACGAACGCCA TCCTTTGTAT AAAAAAATTA AGTTAAGTTT TCCAGTTAAT 
CAAGAAATAG TCCGGCAAAG AGGTTTGGAT GTTGACGACG GTTCCGTATA TGGAATTCCG 
GGCTATATGG ATCCAACAAC TTTTGTTTCA AAAATCACAG TCAAAGCAAT GTACGATTAC 
AAGGCTCGTC GAGACGACGA ATTAACTTTA GTAAAACATG CTATAATTAC GAATGTAAAT 
AGACAACAAA ACGGTGGTTG GTGGAGAGGT GACTATGGTG GTAAGAAGCA GCACTGGTTC 
CCGGCCAATT ACGTTGAAGA AATCGAGCAA GATAATCAGA CGGATTCATC AGATTCGATG 
ATGCTGGGAA ATTTGCAAAA AGGCTCTTTA GACATAATAG GCGCTGTTGT GGAGTTAGCC 
ATGTCCGACC GACCTGGTTT AGAATGGATT TTACGGATAC AGAATCCAAG CATGTGCTCT 
GTTTTCGAGG CAGCAGCTCC ATCCAAAGAT ATCGCCGTCG AATGGGTACA AAAAATTCAC 
GAGACTGGAC AAAACGCCAG CGTCAGGGAG AATCAACACA AAGAAATGGA AAGAGCTTGG 
AGGATAGCAA AAGAAATGTC CAATCTCATA GTTTACTTTA GATCAGTAGC CTTCAACGTG 
GAGCGGCTAC GAGCCAAGGG ATTTATTTTC AACGAAATGA GCAGCTTTCC GGAGACTAAA 
GCAGAAAAGC TTATGTGCCA ACAAGAAAAC AAATTCTTTT TACGTTATCA CCAGGTGCAA 
TTCAGCCGAG TCTATCCCAA GGGCCAACGA ATAGATTCTT CAAATTACAA TCCGGTACCT 
ATGTGGAACT CGGGTTGTCA GATGGTGGCC CTAAATTACC AAACAGGCGA TAAGCCAATG 
CAGCTAAATC AGGCGAAATT TCGAGAGAAC GGTAACTGCG GTTATCTGTT GAAGCCTGAT 
TTTATGTTTC ACGAAGATTT CACTCCGTAC GATAAGAGTT CATTGACCGG AGTGGACACG 
TTAAAGATTA CGCTGCACGT CATAGGTGCA AGACACCTAA GTAAGCCGGG AAGGGGCACA 
GCGAGTCCTT TCGTACAAGT CGAAGTAGTG GGTGCAGATT TCGATAGCGG CGTTAAGTTA 
ACGACTAAGA CTATTACCGA TAACGGTTTC AATCCCATGT GGAACGAATC CTGTGAGTTC 
CTAGTAGCAA ATCCATATTT GGCTATGCTT CGGTTCGTTG TTCAAGACGA GGATATGTTT 
GGAGATAGCA ATTTTATTGG ACAGGCAACT TACCCTATTC GCTGCTTGAG GACAGGTTAC 
AGAAGTGTTG TTCTGAAAAA CAGTTATAGC GAAGACCTTG AATTGGCGTC TCTCCTAGTG 
CATGTGAAAA TTATTAGCTC AGGTGACTTG ATGCACAATA ATACTCCAAA ACACGATGGA 
CGATCCATTG CTCTGAACTC TCGAGCCGTG GACAGAGATT CACAACTTTC TGGAAGATTG 
CACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100123217 ( XM_003427203.3 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_003427251.1 Nasonia vitripennis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100123217), mRNA.

Price & Availability↑

CloneID	ONd14417
Clone ID Related Accession (Same CDS sequence)	XM_003427203.3 , XM_003427203.4
Accession Version	XM_003427203.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001818015.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 26, 2016 this sequence version replaced XM_003427203.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAA TAATTCCTGA AATGGAGCAG ATCATCAGTC AGCTTGAAAG AGGGACTGTG 
GTCACCAAGT TCTTTCCAAG AAAAAGACCA GAAAAGAAGA CATTAATGAT ACGCAGAGAA 
ACCAGACAAA TAGTTTGGGC AAGAAGCACC ACATCAAGAC CTTTTGATGG CTCTGTGGAT 
ATTCGAGAAG TGAAAGAAAT AAGAATTGGA AAGAACTCAA AAGACTTTGA GAAATGGCCA 
GAAGATGCTA AACGAATTGA TAATCTACGT TGTTTTGTAG TTTACTATGG CTCTGAGTTC 
AGACTAAAAA CTCTTTCAAT ATCAGCTCTG AGTGAAAAAG AGTGTGAACT CTGGATTAAA 
GGACTTAGAC ACTTGATGCA AGATACTATA AATGCTCCAT ACCCATTGCA AGTTGAAAGA 
TGGCTAAGAA AAGAATTTTA TGCAATGGAA AATTCCAGAG AAATGGTTAC TTTAAAAGAT 
GTCAAAGCCT TTTTACCAAG AGTAAACTGT AAGATAGCAA CTAACAAACT CAGAGAAGTT 
TTTCAAGAAG TCGATACAAA AAATAGAACT GAAATAGGAT TTGACGATTT TGTGATACTT 
TACCATAAAC TTATGTTTGA TCCAAACGTG AGCAATTTAA CATATTGGAC AAGACTGTTA 
GTTTATTCTA ACTCTGGGAT GTTGGTGACA CTACAAGAAT TTCAAAATTT TCTACTCACA 
GAACAACAAG ATCCTTCAGG AAATATTGAA TCAGACGTTT CGCTTTTTAT TCGCGAGTAT 
CTGCAAGATC CTCAAAGAGA CGTTCAGGAA CCTTTTTTTC ATTTCTCTGA ATTCATAGAT 
TTTTTGTTTT CAAAACAGAA TGAAATTTGG GATAGTCAAT ATGATCATGT CTATCACGAT 
ATGACTAGGC CTATGTCTCA TTATTGGATT GCTTCATCGC ATAATACTTA CTTGATGGGT 
GATCAAATCA GTAGTGAGAG CAGTTGCCAA GCATATGTTA GAGCTCTGCG AGTTGGTTGC 
AGGTGTATAG AACTAGATTG CTGGGATGGA CCAGATGGGA TGCCTTTTAT ATTTCATGGA 
CATACTTTGA CGACGAAAAT CAAATTTTTA GACGTAATTA AAACAATTAA AGAACACGCT 
TTTGTCACTT CCGAATATCC TGTGATTCTC TCAATAGAAG ACAACTGCAC ACTACCGCAA 
CAGCGGAAAA TGGCTGTGGC TATGCAGGAA GTCTTTGGCG ACATGTTGCT GGTTCAACCC 
GTCGACAAGA GTGAATCCAC TTTACCCTCG CCCTACGCTC TGCGAAGAAA AATTCTACTC 
AAGCACAAAA AGCTTCCCGA CGGCGTTGAC GAAAGTTCCT TTGTCATTCG AAACGATGAA 
AGCCGTCAAG AGATGGATTT GAGAAACACT ATTAAAAATG GTATATTGTA TCTGGAGGAT 
CCAGTAGATC GAGAATGGAA TCCACACTTT TTTGTCCTGA CGCAGCAGAA ACTCTTTTAT 
ACAGATACTT TCTCAAAACC GCAAGAAACC GAACCGGACG ACGATGACGA AGCCGTTGTT 
AGAACTCCCG GTGATGGTGT TCCAAACGAT GAATTACATT TTGGAGAAAT GTGGTTCCAT 
GGTAAATTGG CCAGAGGAAG AGAGGAAGCT GAAGAACTTT TACGAAGGTA TTCCAATTTA 
GGAGATGGAA CGTTTTTGGT CAGGCAATGC GTGACCTTTG TGGGAGACTA TTGCTTATCA 
TTTTGGAGGA AAGGAAAAGT CAATCATTGC CGTATCAAGT TAAAACAAGA CATGGGTCAG 
ACACAGTTTT ATTTGATAGA CACTCACTGT TTTGACAGTT TATACAGTCT CATCACTTAT 
TATCGTAATA ATCCTCTGAG AAGCCAGGAA TTTTTAATCA CATTACAAGA ACCTGTACCG 
CAGCCAAGTA AACACGAAGA AAAAGAATGG TGGCACTCTG AATGCTCAAG AACATTGGCC 
GAGGAAATGT TGAAGCGTGT ACCAACCGAT GGAGCGTTTT TAGTGAGGCC AAGCGAAAAG 
GAGAATAATT GTTACGCCAT TTCCTTCAGG GCTGAAAATA AAATAAAGCA TTGCCGCATA 
AAACTTGAAG GACGGCTATA TGCAATCGGA ACAGTGCAAT TTGAAAGTTT GGTGGAATTA 
GTCAATTACT ACGAACGCCA TCCTTTGTAT AAAAAAATTA AGTTAAGTTT TCCAGTTAAT 
CAAGAAATAG TCCGGCAAAG AGGTTTGGAT GTTGACGACG GTTCCGTATA TGGAATTCCG 
GGCTATATGG ATCCAACAAC TTTTGTTTCA AAAATCACAG TCAAAGCAAT GTACGATTAC 
AAGGCTCGTC GAGACGACGA ATTAACTTTA GTAAAACATG CTATAATTAC GAATGTAAAT 
AGACAACAAA ACGGTGGTTG GTGGAGAGGT GACTATGGTG GTAAGAAGCA GCACTGGTTC 
CCGGCCAATT ACGTTGAAGA AATCGAGCAA GATAATCAGA CGGATTCATC AGATTCGATG 
ATGCTGGGAA ATTTGCAAAA AGGCTCTTTA GACATAATAG GCGCTGTTGT GGAGTTAGCC 
ATGTCCGACC GACCTGGTTT AGAATGGATT TTACGGATAC AGAATCCAAG CATGTGCTCT 
GTTTTCGAGG CAGCAGCTCC ATCCAAAGAT ATCGCCGTCG AATGGGTACA AAAAATTCAC 
GAGACTGGAC AAAACGCCAG CGTCAGGGAG AATCAACACA AAGAAATGGA AAGAGCTTGG 
AGGATAGCAA AAGAAATGTC CAATCTCATA GTTTACTTTA GATCAGTAGC CTTCAACGTG 
GAGCGGCTAC GAGCCAAGGG ATTTATTTTC AACGAAATGA GCAGCTTTCC GGAGACTAAA 
GCAGAAAAGC TTATGTGCCA ACAAGAAAAC AAATTCTTTT TACGTTATCA CCAGGTGCAA 
TTCAGCCGAG TCTATCCCAA GGGCCAACGA ATAGATTCTT CAAATTACAA TCCGGTACCT 
ATGTGGAACT CGGGTTGTCA GATGGTGGCC CTAAATTACC AAACAGGCGA TAAGCCAATG 
CAGCTAAATC AGGCGAAATT TCGAGAGAAC GGTAACTGCG GTTATCTGTT GAAGCCTGAT 
TTTATGTTTC ACGAAGATTT CACTCCGTAC GATAAGAGTT CATTGACCGG AGTGGACACG 
TTAAAGATTA CGCTGCACGT CATAGGTGCA AGACACCTAA GTAAGCCGGG AAGGGGCACA 
GCGAGTCCTT TCGTACAAGT CGAAGTAGTG GGTGCAGATT TCGATAGCGG CGTTAAGTTA 
ACGACTAAGA CTATTACCGA TAACGGTTTC AATCCCATGT GGAACGAATC CTGTGAGTTC 
CTAGTAGCAA ATCCATATTT GGCTATGCTT CGGTTCGTTG TTCAAGACGA GGATATGTTT 
GGAGATAGCA ATTTTATTGG ACAGGCAACT TACCCTATTC GCTGCTTGAG GACAGGTTAC 
AGAAGTGTTG TTCTGAAAAA CAGTTATAGC GAAGACCTTG AATTGGCGTC TCTCCTAGTG 
CATGTGAAAA TTATTAGCTC AGGTGACTTG ATGCACAATA ATACTCCAAA ACACGATGGA 
CGATCCATTG CTCTGAACTC TCGAGCCGTG GACAGAGATT CACAACTTTC TGGAAGATTG 
CACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003427251.1
CDS	435..4103
Translation	MNGIIPEMEQIISQLERGTVVTKFFPRKRPEKKTLMIRRETRQIVWARSTTSRPFDGSVDIREVKEIRIGKNSKDFEKWPEDAKRIDNLRCFVVYYGSEFRLKTLSISALSEKECELWIKGLRHLMQDTINAPYPLQVERWLRKEFYAMENSREMVTLKDVKAFLPRVNCKIATNKLREVFQEVDTKNRTEIGFDDFVILYHKLMFDPNVSNLTYWTRLLVYSNSGMLVTLQEFQNFLLTEQQDPSGNIESDVSLFIREYLQDPQRDVQEPFFHFSEFIDFLFSKQNEIWDSQYDHVYHDMTRPMSHYWIASSHNTYLMGDQISSESSCQAYVRALRVGCRCIELDCWDGPDGMPFIFHGHTLTTKIKFLDVIKTIKEHAFVTSEYPVILSIEDNCTLPQQRKMAVAMQEVFGDMLLVQPVDKSESTLPSPYALRRKILLKHKKLPDGVDESSFVIRNDESRQEMDLRNTIKNGILYLEDPVDREWNPHFFVLTQQKLFYTDTFSKPQETEPDDDDEAVVRTPGDGVPNDELHFGEMWFHGKLARGREEAEELLRRYSNLGDGTFLVRQCVTFVGDYCLSFWRKGKVNHCRIKLKQDMGQTQFYLIDTHCFDSLYSLITYYRNNPLRSQEFLITLQEPVPQPSKHEEKEWWHSECSRTLAEEMLKRVPTDGAFLVRPSEKENNCYAISFRAENKIKHCRIKLEGRLYAIGTVQFESLVELVNYYERHPLYKKIKLSFPVNQEIVRQRGLDVDDGSVYGIPGYMDPTTFVSKITVKAMYDYKARRDDELTLVKHAIITNVNRQQNGGWWRGDYGGKKQHWFPANYVEEIEQDNQTDSSDSMMLGNLQKGSLDIIGAVVELAMSDRPGLEWILRIQNPSMCSVFEAAAPSKDIAVEWVQKIHETGQNASVRENQHKEMERAWRIAKEMSNLIVYFRSVAFNVERLRAKGFIFNEMSSFPETKAEKLMCQQENKFFLRYHQVQFSRVYPKGQRIDSSNYNPVPMWNSGCQMVALNYQTGDKPMQLNQAKFRENGNCGYLLKPDFMFHEDFTPYDKSSLTGVDTLKITLHVIGARHLSKPGRGTASPFVQVEVVGADFDSGVKLTTKTITDNGFNPMWNESCEFLVANPYLAMLRFVVQDEDMFGDSNFIGQATYPIRCLRTGYRSVVLKNSYSEDLELASLLVHVKIISSGDLMHNNTPKHDGRSIALNSRAVDRDSQLSGRLHA

Target ORF information:

RefSeq Version	XM_003427203.3
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (LOC100123217), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003427203.3

ORF Insert Sequence:

ATGAATGGAA TAATTCCTGA AATGGAGCAG ATCATCAGTC AGCTTGAAAG AGGGACTGTG 
GTCACCAAGT TCTTTCCAAG AAAAAGACCA GAAAAGAAGA CATTAATGAT ACGCAGAGAA 
ACCAGACAAA TAGTTTGGGC AAGAAGCACC ACATCAAGAC CTTTTGATGG CTCTGTGGAT 
ATTCGAGAAG TGAAAGAAAT AAGAATTGGA AAGAACTCAA AAGACTTTGA GAAATGGCCA 
GAAGATGCTA AACGAATTGA TAATCTACGT TGTTTTGTAG TTTACTATGG CTCTGAGTTC 
AGACTAAAAA CTCTTTCAAT ATCAGCTCTG AGTGAAAAAG AGTGTGAACT CTGGATTAAA 
GGACTTAGAC ACTTGATGCA AGATACTATA AATGCTCCAT ACCCATTGCA AGTTGAAAGA 
TGGCTAAGAA AAGAATTTTA TGCAATGGAA AATTCCAGAG AAATGGTTAC TTTAAAAGAT 
GTCAAAGCCT TTTTACCAAG AGTAAACTGT AAGATAGCAA CTAACAAACT CAGAGAAGTT 
TTTCAAGAAG TCGATACAAA AAATAGAACT GAAATAGGAT TTGACGATTT TGTGATACTT 
TACCATAAAC TTATGTTTGA TCCAAACGTG AGCAATTTAA CATATTGGAC AAGACTGTTA 
GTTTATTCTA ACTCTGGGAT GTTGGTGACA CTACAAGAAT TTCAAAATTT TCTACTCACA 
GAACAACAAG ATCCTTCAGG AAATATTGAA TCAGACGTTT CGCTTTTTAT TCGCGAGTAT 
CTGCAAGATC CTCAAAGAGA CGTTCAGGAA CCTTTTTTTC ATTTCTCTGA ATTCATAGAT 
TTTTTGTTTT CAAAACAGAA TGAAATTTGG GATAGTCAAT ATGATCATGT CTATCACGAT 
ATGACTAGGC CTATGTCTCA TTATTGGATT GCTTCATCGC ATAATACTTA CTTGATGGGT 
GATCAAATCA GTAGTGAGAG CAGTTGCCAA GCATATGTTA GAGCTCTGCG AGTTGGTTGC 
AGGTGTATAG AACTAGATTG CTGGGATGGA CCAGATGGGA TGCCTTTTAT ATTTCATGGA 
CATACTTTGA CGACGAAAAT CAAATTTTTA GACGTAATTA AAACAATTAA AGAACACGCT 
TTTGTCACTT CCGAATATCC TGTGATTCTC TCAATAGAAG ACAACTGCAC ACTACCGCAA 
CAGCGGAAAA TGGCTGTGGC TATGCAGGAA GTCTTTGGCG ACATGTTGCT GGTTCAACCC 
GTCGACAAGA GTGAATCCAC TTTACCCTCG CCCTACGCTC TGCGAAGAAA AATTCTACTC 
AAGCACAAAA AGCTTCCCGA CGGCGTTGAC GAAAGTTCCT TTGTCATTCG AAACGATGAA 
AGCCGTCAAG AGATGGATTT GAGAAACACT ATTAAAAATG GTATATTGTA TCTGGAGGAT 
CCAGTAGATC GAGAATGGAA TCCACACTTT TTTGTCCTGA CGCAGCAGAA ACTCTTTTAT 
ACAGATACTT TCTCAAAACC GCAAGAAACC GAACCGGACG ACGATGACGA AGCCGTTGTT 
AGAACTCCCG GTGATGGTGT TCCAAACGAT GAATTACATT TTGGAGAAAT GTGGTTCCAT 
GGTAAATTGG CCAGAGGAAG AGAGGAAGCT GAAGAACTTT TACGAAGGTA TTCCAATTTA 
GGAGATGGAA CGTTTTTGGT CAGGCAATGC GTGACCTTTG TGGGAGACTA TTGCTTATCA 
TTTTGGAGGA AAGGAAAAGT CAATCATTGC CGTATCAAGT TAAAACAAGA CATGGGTCAG 
ACACAGTTTT ATTTGATAGA CACTCACTGT TTTGACAGTT TATACAGTCT CATCACTTAT 
TATCGTAATA ATCCTCTGAG AAGCCAGGAA TTTTTAATCA CATTACAAGA ACCTGTACCG 
CAGCCAAGTA AACACGAAGA AAAAGAATGG TGGCACTCTG AATGCTCAAG AACATTGGCC 
GAGGAAATGT TGAAGCGTGT ACCAACCGAT GGAGCGTTTT TAGTGAGGCC AAGCGAAAAG 
GAGAATAATT GTTACGCCAT TTCCTTCAGG GCTGAAAATA AAATAAAGCA TTGCCGCATA 
AAACTTGAAG GACGGCTATA TGCAATCGGA ACAGTGCAAT TTGAAAGTTT GGTGGAATTA 
GTCAATTACT ACGAACGCCA TCCTTTGTAT AAAAAAATTA AGTTAAGTTT TCCAGTTAAT 
CAAGAAATAG TCCGGCAAAG AGGTTTGGAT GTTGACGACG GTTCCGTATA TGGAATTCCG 
GGCTATATGG ATCCAACAAC TTTTGTTTCA AAAATCACAG TCAAAGCAAT GTACGATTAC 
AAGGCTCGTC GAGACGACGA ATTAACTTTA GTAAAACATG CTATAATTAC GAATGTAAAT 
AGACAACAAA ACGGTGGTTG GTGGAGAGGT GACTATGGTG GTAAGAAGCA GCACTGGTTC 
CCGGCCAATT ACGTTGAAGA AATCGAGCAA GATAATCAGA CGGATTCATC AGATTCGATG 
ATGCTGGGAA ATTTGCAAAA AGGCTCTTTA GACATAATAG GCGCTGTTGT GGAGTTAGCC 
ATGTCCGACC GACCTGGTTT AGAATGGATT TTACGGATAC AGAATCCAAG CATGTGCTCT 
GTTTTCGAGG CAGCAGCTCC ATCCAAAGAT ATCGCCGTCG AATGGGTACA AAAAATTCAC 
GAGACTGGAC AAAACGCCAG CGTCAGGGAG AATCAACACA AAGAAATGGA AAGAGCTTGG 
AGGATAGCAA AAGAAATGTC CAATCTCATA GTTTACTTTA GATCAGTAGC CTTCAACGTG 
GAGCGGCTAC GAGCCAAGGG ATTTATTTTC AACGAAATGA GCAGCTTTCC GGAGACTAAA 
GCAGAAAAGC TTATGTGCCA ACAAGAAAAC AAATTCTTTT TACGTTATCA CCAGGTGCAA 
TTCAGCCGAG TCTATCCCAA GGGCCAACGA ATAGATTCTT CAAATTACAA TCCGGTACCT 
ATGTGGAACT CGGGTTGTCA GATGGTGGCC CTAAATTACC AAACAGGCGA TAAGCCAATG 
CAGCTAAATC AGGCGAAATT TCGAGAGAAC GGTAACTGCG GTTATCTGTT GAAGCCTGAT 
TTTATGTTTC ACGAAGATTT CACTCCGTAC GATAAGAGTT CATTGACCGG AGTGGACACG 
TTAAAGATTA CGCTGCACGT CATAGGTGCA AGACACCTAA GTAAGCCGGG AAGGGGCACA 
GCGAGTCCTT TCGTACAAGT CGAAGTAGTG GGTGCAGATT TCGATAGCGG CGTTAAGTTA 
ACGACTAAGA CTATTACCGA TAACGGTTTC AATCCCATGT GGAACGAATC CTGTGAGTTC 
CTAGTAGCAA ATCCATATTT GGCTATGCTT CGGTTCGTTG TTCAAGACGA GGATATGTTT 
GGAGATAGCA ATTTTATTGG ACAGGCAACT TACCCTATTC GCTGCTTGAG GACAGGTTAC 
AGAAGTGTTG TTCTGAAAAA CAGTTATAGC GAAGACCTTG AATTGGCGTC TCTCCTAGTG 
CATGTGAAAA TTATTAGCTC AGGTGACTTG ATGCACAATA ATACTCCAAA ACACGATGGA 
CGATCCATTG CTCTGAACTC TCGAGCCGTG GACAGAGATT CACAACTTTC TGGAAGATTG 
CACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100123217 cDNA ORF clone, Nasonia vitripennis(jewel wasp)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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