SMG1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMG1
Entrez Gene ID	100078545
Full Name	SMG1, nonsense mediated mRNA decay associated PI3K related kinase
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007657654.2	XP_007655844.1	serine/threonine-protein kinase SMG1
XM_029058053.1	XP_028913886.1	serine/threonine-protein kinase SMG1 isoform X1
XM_029058054.1	XP_028913887.1	serine/threonine-protein kinase SMG1 isoform X2

Pathways from BioSystems
Gene Ontology

The following SMG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc00605	XM_007657654.2 Latest version!	Ornithorhynchus anatinus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OOc122577	XM_029058053.1 Latest version!	Ornithorhynchus anatinus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OOc122578	XM_029058054.1 Latest version!	Ornithorhynchus anatinus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMG1 ( XM_007657654.2 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007655844.1 Ornithorhynchus anatinus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), mRNA.

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CloneID	OOc00605
Clone ID Related Accession (Same CDS sequence)	XM_007657654.2
Accession Version	XM_007657654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	serine/threonine-protein kinase SMG1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009095.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 15, 2016 this sequence version replaced XM_007657654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCACCA GCCGACCAGG ATCATTTAAA ATATTCTTCG 
TCTAGAGATC GGGGCAGTTC TGCCTCTTAT GGATTACAAC CTTCAAATTC AGTTGTGGTG 
TCTCGTCAAC GGCACGATGA TATCAGAGTC CACACCGACA TACAGAATGA AGAAAAGGGT 
GGCTACAATG TCAATGGAGG AGCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAACCCT GATTTCACCA GTGTTCAGCA TGGAAATCGT 
GCTTTAGCCA CAAAAGACAT GAGGAAATCA CAGGAGCGAT CGTTGTCTTA CTCTGACGAG 
TCTCGACTGT CAAACCTTCT TCGGAGGATT ACCCGGGAAG ACGACAGAGA CCGAAGACTG 
GCTACTGTGA AGCAATTGAA AGAATTCATT CAGCAACCAG AAAATAAGCA GGTCTTAGTT 
AAACAACTGG ATAATATCCT GACCGCTATA CATGATGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAAT TGAGGCAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTTTGAGT 
TATGAGGCCG AGAAGATCTT CAAATGGATT TTTAGCAAAT TTAGCTCGTC CACAAAAGAT 
GAAGTTAAAC TGCTTTACTT ATGTGCCACC TTCAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCCAT TCTAGAAAAT 
GTGGATACAC CAGAATTGCT ATGCAAATGT GTGAAGTGCA TTCTTCTGGT GGCCCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACGGTTG ACATATTGGT TGGGTGGCAT 
ATAGATCACA CTCAGAAACC TTCCTTGACA CAGCAAGTGT CTGGATGGCT GCAGAGTCTG 
GAGCCCTTTT GGGTAGCCGA TCTTGCATTT TCCACCACCC TTCTCGGTCA GTTTCTGGAA 
GATATGGAGG CTTATGCTGA AGATCTCAGT CATGTGGCAT CTGGGGAATC GGTCGATGAA 
GACATTCCTC CTCCATCAGT GTCCTTACCG AAGTTGGCGG CTCTTCTCCG GGTTTTCAGT 
ACTGTGGTGC GGAGTATTGG GGAGCGCTTC AGTCCAATTA GAGGACATCC AATTACCGAG 
GCTTATGTAA CAGATGTTTT GTACAGAGTA ATGAGATGTG TGACAACAGC AAACCAAGTA 
TTTTTTTCTG AAGCTGTATT GACAGCTGCC AATGAATGTG TCGGTGTATT GCTTGGTAGC 
CTAGATCCTA GTATGACTAT TCATTGTGAC ATGGTCATCA CCTATGGACT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACAGATTAT ATCATCTCGG TCTTAAATTT ACTGACGTTG 
ATTGTTGAGC AGATCAATAC AAAGCTGCCA TCGTCATTCG TCGAAAAACT GTTTATACCA 
GCGTCTAAAC TACTGCTTCT GCGCTATCAT AAAGAAAAAG AGGTTGTTGC AGTAGCTCAT 
GCTGTATATC AAGCCGTTCT TAGCCTGAAG AACATTCCCG TCTTGGAGAC CGCCTATAAG 
TTAATTTTAG GAGAAATGAC TTGTGCACTG AATAATCTCC TGCATAACCT TCAACTTCCT 
GAGGCCTGTT CCGAAATCCA ACACGATGCT TTTAAGAATC ACGTTTTCAA TGTGGACAAT 
GCCAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACCA CGATTGGAAA TGCGAAAAAC 
TCATTAATTG GGATGTGGGC ACTCTCGCCA ACCGTTTTTG CACTTCTGAG CAAGAATCTG 
ATGATAGTGC ATGGAGATCT CGCCGTTCAC TTCCCAGCTA TTCAGTATGC CGTTCTCTAC 
ACTTTGTATT CACATTGTAC CAGACATGAT CACTTCATTT CTAGCAGTCT TAGCTCCTCT 
TCTCCTTCTC TCTTTGATGG AGCAGTTATC AGCACTGTCA CCACAGCTAC AAAGAAGCAT 
TTCTCAATCA TATTAAACCT CCTGGGAATA TTACTGAAGA AAGATAATCT TAATCAGGAT 
ACCAGGAAAC TACTAATGAC CTGGGCTTTG GAAGTTGCTG TTCTAATGAA AAAGTCTGAA 
ACATATGCAC CTTTGTTCTC TCTTCCGTCG TTCCATAAAT TTTGCAAAGG ACTTCTAGCA 
AACACTCTTC TTGAAGATGT GAATATCTGC CTTCAAGCAT GTAGTAGTCT GCATGCCTTG 
TCATCTTCCC TGCCAGATGA CCTTTTACAA AGGTGTGTTG ATGTGTGCCG CGTTCAGCTG 
GTCCATAGTG GAACTCGGAT TCGACAAGCC TTTGGAAAGC TGTTGAAGTC TATTCCGTTA 
GATGTTGTCT TGAGCAATAG CAACCACACA GAGATTCAAG AAATTTCCTT AGCTATAAGG 
AGTCACATGA GCAAAACTCC AAGTAATACT TTCCATCCAC AAGATTTCTC TGACGTGATA 
AGTTTTATTT TGTATGGAAA CTCTCATAGA ACCGGGAAGG ACAATTGGTT AGAAAGATTG 
TTCTACAGCT GTCAGAGATT GGATAAGCGT GATCAGTCAA CCATTCCACG CAATCTACTC 
AAAACAGATG CCGTCCTCTG GCAGTGGGCT ATTTGGGAAG CTGCTCAGTT CACTGTTCTC 
TCTAAACTAA GAACCCCGCT GGGCAGAGCT CAAGATACCT TCCAGACAAT CGAAGGTATC 
ATTAGAAGTC TTGCCGCTCA TACATTAAAC CCCGATCAAG ATGTTAGCCA GTGGACCACT 
GCGGACAATG ATGAAGGACA TAGCAGCAAC CAACTCAGAC TTGTTCTTCT ACTGCAGTAC 
TTGGAAAATT TGGAAAAACT AATGTATAAT GCATATGAAG GTTGTGCTAA TGCACTGACT 
TCCCCTCCTA AGGTTATCAG GACATTTTTC TATACCAATC GCCAGACATG TCAGGACTGG 
TTAACTCGGA TTAGACTTTC CATCATGAGA GTTGGATTGT TGGCCGGTCA GCCCGCTGTG 
ACAGTAAGAC ACGGTTTTGA TTTGCTCACC GAAATGAAGA CCAATAGTAT CTCTCAGGGA 
AATGAGCTAG AAGTGATTAT TATGATGGTC GTAGAAGCAC TTTGTGAGCT TCACTGTCCT 
GAAGCTATAC AGGGATTAGC TGTCTGGTCT TCAGCAGTGG TTGGAAAGAG TCTCGTCTGG 
ATTAATTCAG TGGCACAGCA AGCCGAAGGG CGGTTTGAAA AGGCAACTGT TGAATACCAG 
GAGCACTTGT GTGCTATGAC AGGGGTAGAT TGCTGCATAA CTGGGTTTGA TAAATCCATT 
CTGAAGTTGG CTAACATGAA TACTAACAAT ACCAGCCCCA AACATTCTCT CAATGGTGAC 
GCTAGGAAAA CTGTTCTGGC CAAACCAAGT GATTCTTCTC CTGAGGTTAT TAATTACTTG 
GGTAACAAAG CATGTGAGTG CTACATCTCT CTTGCTGATT GGGCTGCTGT GCAGGAATGG 
CAGAATGCTG TCCATGACTT GAAAAAGAGC ACAAGTAGTA CTTCCCTCAA TTTAAAAGCT 
GACTTTAACT ACATAAAATC ACTGAGCAGC TTTGAATCTG GCGAATTTCT CGAATGTACA 
GAACAACTAG AATTGCTGCC AGGAGAAAAC ATTAACTTAC TTGCCAGCGG GTCAAAGGAA 
AAAATAGATA TGAAAAAGCT ACTTCCAAAC ATGTTAAGCC CAGATCCACG GGAACTTCAG 
AAATCCATTG AAGTTCAGTT GCTGAGAAGT TCCGTTTGTT TGGCTACCGC TGTAAGCCAC 
ATAGAGCAGG AACAAAAGTG GCAATCTATA ACTGAAAACG TTGTAAAATA TTTGAAGCAA 
ACATCAAGAA TAGCTATTGG ACCACTGAGA CTTTCCACTT TAACTGTTTC ACAGTCCTTA 
CCAGTTCTAA GTACCCTACA GCTGTATTGT TCTTCTGCTT TGGAGAACAC CGTGTCAAAC 
AGGCTTTCGA CAGAGGACTG CTTAATCCCA CTCTATAGTG AAGCTTTACG TTCGTGTAAA 
CAGCATGATG TAAGGCCCTG GATGCAGGCT TTGAGGTATA CAGTATACCA GAGTCAGTTG 
GTGGAAAAAA TCAAAGAACA AACGGTTCCA ATTAGAAGTC ACCTCATGGA ATTGGGTTTA 
ACAGCTGCGA AATTTGCTCG TAAACGTGGA AACGTTGCCC TGGCAACCAG ATTGCTCGCT 
CAATGTAGTG AAGTGCGGTT GGGAAAGACG ACCACTGCTC AAGATTTAGT CCAGTATTTT 
AAAAAACTGT CAACACAAGG TCAAATAGAT GAAAAATGGG GACCTGAACT GGATATTGAG 
AAAACGAAAT TGTTATACAC AGCAGGTCAG TCAACCCATG CGATGGAAAT GCTGAGTTCT 
TGCGCCATAT CCTTCTGCAA GTCTGCCAAA GCTGAATACG CAGTAGCCAA ATCGATTCTT 
ACACTTGCTA AATGGATCCA AGCTGAATGG AAAGAAATTT CAGGACAGTT GAAACAGGTG 
TACAGAGCCC AACAGCAGCA GAACACCACG GGCCTGTCTA CATTATCTAA AAACATACAC 
ACTTTGATCG AACTGCCGTC TGTCAACACC ACAGAAGAGG AGTATCCCCG CATCGAGAGT 
GAATCAACAG TCAACATTGG AGTTGGGGAA CCGGACTTCA TCCTGGGCCA GTTGTATCAC 
CTGTCTTCAG TGCAGGCACC CGAAGTGGCC AAATCTTGGG CAGCGCTGGC AAGTTGGGCT 
TACAGATGGG GAAGAAAGGT GGTGGATAAC GCCAGTCAGG GAGAAGGTGT TCGACTACTG 
CCAAGAGAGA AATCTGAGGT TCAAAATCTG CTTCCAGACA ACATAACAGA GGAGGATCGA 
GAGAAGATCT ACGGCATCCT TGGGCAGGCG GTGTGTCGGC CAGCCGGAAT TCAGGATGAA 
GATATAACAC TGCAGATAAC AGAGAGTGAA GACAATGAGG AAGATGACAT GGTGGATGTG 
ATCTGGCGTC AGTTATTGAC GAGTTGCCCG TGGCTTTCCG ATCTTGATGA AAATGCCACA 
GAAGGCCTAA TTAAAGTGTG GAGAAAAATT GTAGATAGGA TATTCAGTCT CTACAAACTG 
TCCTGCAGTG CCTATTTTAC TTTCCTTAAA CTCAATGCTG GCCAGGTTAC TCTAGATGAA 
GATGACCCAA GGCTGCACTT GAGCATTAGG TCTGAACAAA GCACTGACGA TGTTATTGTA 
ATGGCAACGT TAAGACTGTT GAGATTGCTG GTCAAACACG CTGGAGAGCT AAGGCAGTAT 
CTGGAGCACG GCCTAGAAAC CACCCCCACT GCTCCTTGGA GAGGTATCAT TCCACAGCTC 
TTCTCCCGTC TAAATCATCC CGAAGTGTAC GTCCGCCAAA GTATTTGTAA TCTACTCTGT 
CGAGTGGCAC AAGATTCTCC TCATCTCATA TTATATCCTG CAATTGTGGG AACTATTTCA 
CTGAGTAGTG AATCGCAGAC ATCAGGCAAT AAGTTGCCTT CAGCCATCCC CACTTTGCTG 
GGCAATATTC AAGGCGAAGA ATTACTCGGA GCGGAATGTG AAGGGGGAAG TCCTCCAGCT 
TCTCAAGACA GCAATAAAGA TGAACCCAAG AATGGCTTAA ATGAAGATCA AGCGATGATG 
CAGGATTGCT ACAGCAAAAT TGTTGATAAA TTGTCTTCTG CCAACCCAAC AATGGTCTTA 
CAGGTTCAAA TGCTGGTGGC CGAGTTGCGC AGGGTGACCG TTCTTTGGGA TGAGCTCTGG 
CTGGGAGTTC TTCTACAACA ACATATGTAT GTTCTGAGGC GCATTCAACA GTTGGAGGAC 
GAAGTAAAGA GAGTCCAGAA TAACAATACG TTACGCAAAG AAGAGAAGAT TGCAATAATG 
CGAGAAAAGC ACACAGCTCT GATGAAGCCC ATCGTATTTG CTTTGGAACA TGTACGGAGC 
ATCACTGCCG CTCCTGCCGA AACACCTCAT GAGAAGTGGT TCCAAGACAA CTATGGTGAG 
GCAATTGAAA ATGCCCTGGA AAAACTCAAG AACCCATTAA ATCCTGCAAA ACCCGGAAGC 
AGTTGGATCC CATTTAAAGA GGTGATGCTA AGTTTGCAGC AGAGAGCACA GAAACGTGCT 
AGTTATATCT TGCGCCTTGA AGAAATCAGC CCCTGGTTGG CTGCCATGAC TAATACTGAA 
ATTGCCCTTC CTGGAGAAGT ATCAGCCAGA GATACCGTGA CGATCCACAG CGTGGGCGGA 
ACCATCACAA TCCTACCTAC TAAAACCAAA CCCAAAAAAC TGCTCTTTCT GGGTTCAGAC 
GGGAAAAACT ACCCCTATCT CTTTAAAGGA CTGGAAGATC TGCACCTGGA TGAGAGAATC 
ATGCAGTTTC TATCTATCGT TAATACCATG TTCGCTACCA TCAATCGCCA AGAGACGCCC 
CGTTTCCATG CCAGGCACTA TTCGGTGACC CCGCTGGGGA CGAGGTCGGG CCTGATTCAG 
TGGGTAGATG GAGCCACTCC CTTATTTGGC CTTTACAAGC GATGGCAACA GCGAGAAGCT 
GCTTTACAAG CGCAAAAGGC CCAGGATTCT TACCAGACTC CTCAAAATCC TGGAATAGTA 
CCTCGGCCCA GCGAGCTTTA TTACAGTAAA ATCGGGCCCG CCCTGAAAGC GGTCGGACTC 
AGTCTCGACG TGTCCCGGCG AGACTGGCCT CTCAACGTGA TGAAGGCTGT GTTGGAAGAA 
CTGATGGAAG CCACCCCTCC AAACCTTCTG GCCAAGGAGC TTTGGTCTTC TTGTACCACA 
CCTGACGAAT GGTGGAGAGT TACCCAGTCT TACGCAAGAT CCACCGCGGT CATGTCAATG 
GTTGGATACA TAATCGGCCT TGGAGATAGA CATCTGGATA ACGTTCTTAT AGACATGACT 
ACAGGAGAAG TTGTTCACAT AGATTACAAT GTTTGTTTTG AAAAAGGTAA AAGTCTGAGA 
GTGCCGGAGA AAGTTCCTTT CAGGATGACT CACAACATCG AAACAGCTCT CGGTGTAACT 
GGAGTTGAGG GAGTTTTCAG GCTTTCTTGT GAACAGGTTC TCCAAATTAT GCGGCGTGGA 
AGAGAGACCC TGCTCACGTT GCTGGAAGCC TTTGTGTACG ATCCTCTTGT GGACTGGACA 
GCCGGAGGGG AAGCAGGATT TGCAGGAGCT GTCTATGGCG GAGGCGGCCA GCAGGCAGAG 
AACAAGCAAA GTAAAAGGGA GATGGAAAGG GACATCACCC GCAGCCTCTT TTCTTCCCGA 
GTAGCTGAAA TCAAGGTGAA CTGGTTTAAA AACAGAGATG AGATGTTGAT TGTGCTTCCA 
AAGCTAGATG GAAGCTTAGA AGAATACCTG AGCCTACAAG AGCAATTGAC AGATGTTGAA 
AAACTGCAAG GCAAACTCGT TGAGGAGATT GACTTTCTAG AAGGAGCCGA AGGAGTAGAT 
CATCCCTCGC ACACTCTGCA GCACAGGTAT TCCGAACACA CCCAACTGCA GACTCAGCAG 
AGAACCGTTC AAGAAGCCAT CCAGGTGAAG TTGAATGAAT TTGAGCAGTG GATAACACAT 
TACCAAGCTG CGTTTAACAA TTTAGAAGCC ACCCAGCTGG CCAGTCTGCT TCAGGAGATT 
AGCACCCAAA TGGACCTGGG TCCTCCAAGC TACGTGCCTG CAACCACCTT TCTGCAGAAC 
GCTGGCCAAG CCCACTTGAT CAGTCAGTGC GAGCAGCTGG AGGGCGAGGT TGGAGCTTTC 
CTTCAGCAGA GGCGCTCCGT CCTGCGAGGC TGTCTCGAGC AGCTGCATAA CTACGCGACC 
GTAGCCCTTC AGTACCCGAA AACCATATTC CAGAAACACC GAATCGAGCA GTGGAAGACC 
TGGATGGAAG AACTTATACG CAATACGACC ATCGAGAGAT GTCAGGAGTT GTACCGGAAA 
TATGAACTCC AGTATGCCCC CCAGCCACCA CCCACGGTGT GTCAGTTCAT AACTGCCACT 
GAAATGACTC TTCAGCGCTA CGCGGCAGAC ATAAATAATA GGCTTATCAG ACAAGTGGAG 
CGCCTGAAAC AAGAAGCAGT GACCGTGCTG GTCTGCGAGG AGCAGTTAAA AGAAATTGAA 
CGGTGTATTA AGGTTTTTCT TCATGAAAAC GGAGAGGAGG GGTCCTGGAG CCTGGCGAGC 
GTTATCATCT CTGCACTCTG TGCACTTACA AGGCGCAACT TAATGATGGA AGGTGCCGCT 
TCTAGTGCTG GCGAGCAGCT GGTCGATCTG ACTTCCCGAG ATGGGGCCTG GTTCTTGGAG 
GAGCTGTGCA GCATGAGTGG AAATGTAACC TGCCTTGTAC AGTTACTGAA GCAGTGTCAC 
CTGGTCCCTC AGGATTTAGA TATTCCCAAT CCCCTCGAAG CTTCGGAGGC AGTTCACTTA 
GCTAATGGAG TCTACACCTC GCTTCAGGAA TTGAACTCCA ACTTCCGGCA GATCATCTTT 
CCGGAAGCTC TGCGATGTTT AATGAAAGGG GAATACACTT TAGAAAGCAT GCTGCACGAA 
CTGGACAACC TCATTGAACA GACAACTGAT GGAGTTCCGT TGCAAACCCT GGTTGACTCT 
CTTCAGGCCT ATTTAAGAAA TGCAGCTATG GGCCTGGAAG AAGAGACACA TTCTCACTAT 
ATTGATGTTG CCAGAATACT CCATTCTCAG TATGGCGAAT TGATTCAACC AAGGAATGGT 
TCAGTTGATG AAACACCAAA AATGTCCGCC GGTCAAATGC TTTTGGTTGC GTTCGATGGA 
ATGTTCGCCC AAGTGGAAAC CGCTTTTGGC TTGTTAATTG AAAAGTTAAA CAAGATGGAA 
GTCCCCGTAT CGTGGCGAAA GATAGACATC ATAAGAGAAG CCAGAAGCAC CCAGATTAAT 
TTCTTTGACG ACGATAATCA CCGACAGGTT CTGGAGGAGA TTTTCTTTCT CAAAAGACTG 
CAGACCATCA AAGAATTCTT TAGACTCTGT GGTACTTTTT CTAAAACGCT GTCAGGATCG 
AGTTCGCTCG AAGATCAGAG CCCGGTGAAT GGACCCGTTC AAATTGTTAA TGTGAAAACG 
CTTTTCAGAA ACTCTTGTTT TAGTGAAGAT CAGATGGCCA AACCCATAAA GGCGTTCACA 
GCTGACTTCG TGAGGCAGCT CTTAATTGGC CTGCCTAATC AAGCCTTGGG GCTAACCCTT 
TGCAGTTTCA TCAGCGCGCT GGGCGTCGAC ATCATCGCCC AGGTCGAGGC AAAGGACTTT 
GGGGCAGAGA GCAAGGTTTC GGTGGACGAT CTGTGTAAGA AAGCCGTGGA GCACAACATC 
CAGATTGGAA AGTTCTCCCA GCTGGTCATG AACCGAGCCA CGGTCCTGGC CAGTTCCTAC 
GACACTGCTT GGAAGAAGCA CGATTTGGTG CGCAGGCTAG AAACCAGTAT TTCCTCTTTC 
AAGACCAGCC TGCAGAGAGT GCAGCTCCAG ATCGCCATGT TTCAGTGGCA GCACGAAGAT 
CTCCTAATCA CCCGACCACA AGCCATGTCT GTGACTCCTC CACCTCGCTC TGCTATTCTA 
ACAAATATGA AAAAGAAGCT GCATACCCTG AGCCAGATCG AAACGTCCAT TGCAGCGGTT 
CAGGAGAAAC TAGCAGCCCT CGAAGCAAGC ATCGAACAAC GTTTGAAGTG GGCAGGAGGG 
GCCAATCCCG CATTGGCTCC AGTCCTACAA GATTTTGAGG CAACAATAGC GGAAAGAAGG 
AATCTCGTTC TGAAAGAAAG CCAACGAGCA AATCAGGTCA CTTTCCTCTG CAGCAATATC 
ATTCATTTTG AAAGCCTACG AACGCGAACT GCCGAAGCTC TAAATCTCGA TGCTGCCTTG 
TTTGAACTCA TCAAGCGCTG TCAACAGATG TGTTCGTTTG CCTCACAGTT TAACAGCTCG 
GTTTCTGAAC TGGAACTGCG TTTACTGCAA AGAGTGGGTT CCGGTCTCGA ACCTCCCATT 
GGCAGCCCTG AATGGCTTGT TTCTGCACAC AAACAACTGA CCCAGGATAT GTCTACTCAG 
AGGGCGGTTC AAACAGAAAA AGAACAGCAA ATCGAATCAG TCTGTGAAGC AATTCAGAAC 
CTTGTGGATA GTATTAAGAC AGTGCTTACC GGTCACAACA GGCAACTTGG AGATGTTAAA 
CATCTATTGA AAGCAATGGC CAAGGATGAA GAGGCTGCTT TGGCAGATGG TGAAGATGTT 
CCCTATGAGA ACAGTGTTAG ACAATTCTTG GGTGAATATA AGTCATGGCA AGACAATATT 
CAAACGGTAT TGTTTACATT AGTTCAGGCT ATGGGGCAGG TTCGCAGCCA AGAGCATGTG 
GAGATGCTGC AGGAGATAAC TCCCACCTTG AAAGAGCTGA AAACACAAAG CCAGAGTATC 
TACAATAATC TAGTGAGTTT TGCATCACCC TTAGTCACAG ATGCAACAAA TGAGTGTTCC 
AGCCCAACAT CATCTGCTAC GTATCAGCCA ACCTTTGCTG CAGCGGTCCG CAGTAACACT 
GGCCAGAAGA CTCAGCCTGA AGTTATGTCG CAGAATGCAA GGAAGCTGAT TCAGAAAAAT 
CTTGCAACAT CAGCAGATAC CCCACCAAGC ACAGTTCCAG GAACTGGCAA GAGTGTGGCC 
TGTAGTCCTA AAAAAGCAGT CAGGGATCCA AAAACTGGAA AAGCTGTGCA AGAAAGGAAC 
TCCTATGCAG TGAGTGTGTG GAAGCGAGTC AAAGCCAAGT TAGAGGGCCG AGATGTGGAT 
CCGAACAGGA GGATGTCTGT TGCTGAACAG GTGGACTATG TGATTAAGGA AGCAACTAAT 
CTAGATAACT TGGCTCAGCT GTATGAAGGT TGGACGGCCT GGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007655844.1
CDS	27..10934
Translation	MRFLRTDSAPADQDHLKYSSSRDRGSSASYGLQPSNSVVVSRQRHDDIRVHTDIQNEEKGGYNVNGGAGENTYGRKSLGQELRVNNVTNPDFTSVQHGNRALATKDMRKSQERSLSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKQVLVKQLDNILTAIHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSTKDEVKLLYLCATFKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGHPITEAYVTDVLYRVMRCVTTANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPASKLLLLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHNLQLPEACSEIQHDAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLLEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNSNHTEIQEISLAIRSHMSKTPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNSISQGNELEVIIMMVVEALCELHCPEAIQGLAVWSSAVVGKSLVWINSVAQQAEGRFEKATVEYQEHLCAMTGVDCCITGFDKSILKLANMNTNNTSPKHSLNGDARKTVLAKPSDSSPEVINYLGNKACECYISLADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFLECTEQLELLPGENINLLASGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATAVSHIEQEQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLYSEALRSCKQHDVRPWMQALRYTVYQSQLVEKIKEQTVPIRSHLMELGLTAAKFARKRGNVALATRLLAQCSEVRLGKTTTAQDLVQYFKKLSTQGQIDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNTTGLSTLSKNIHTLIELPSVNTTEEEYPRIESESTVNIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDNITEEDREKIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLLTSCPWLSDLDENATEGLIKVWRKIVDRIFSLYKLSCSAYFTFLKLNAGQVTLDEDDPRLHLSIRSEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQTSGNKLPSAIPTLLGNIQGEELLGAECEGGSPPASQDSNKDEPKNGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGEAIENALEKLKNPLNPAKPGSSWIPFKEVMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKNYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLNVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGVFRLSCEQVLQIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLIVLPKLDGSLEEYLSLQEQLTDVEKLQGKLVEEIDFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRTVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATTFLQNAGQAHLISQCEQLEGEVGAFLQQRRSVLRGCLEQLHNYATVALQYPKTIFQKHRIEQWKTWMEELIRNTTIERCQELYRKYELQYAPQPPPTVCQFITATEMTLQRYAADINNRLIRQVERLKQEAVTVLVCEEQLKEIERCIKVFLHENGEEGSWSLASVIISALCALTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPLEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDNLIEQTTDGVPLQTLVDSLQAYLRNAAMGLEEETHSHYIDVARILHSQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLIEKLNKMEVPVSWRKIDIIREARSTQINFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQSPVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSFKTSLQRVQLQIAMFQWQHEDLLITRPQAMSVTPPPRSAILTNMKKKLHTLSQIETSIAAVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRANQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVGSGLEPPIGSPEWLVSAHKQLTQDMSTQRAVQTEKEQQIESVCEAIQNLVDSIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPTFAAAVRSNTGQKTQPEVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_007657654.2
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007657654.2

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCACCA GCCGACCAGG ATCATTTAAA ATATTCTTCG 
TCTAGAGATC GGGGCAGTTC TGCCTCTTAT GGATTACAAC CTTCAAATTC AGTTGTGGTG 
TCTCGTCAAC GGCACGATGA TATCAGAGTC CACACCGACA TACAGAATGA AGAAAAGGGT 
GGCTACAATG TCAATGGAGG AGCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAACCCT GATTTCACCA GTGTTCAGCA TGGAAATCGT 
GCTTTAGCCA CAAAAGACAT GAGGAAATCA CAGGAGCGAT CGTTGTCTTA CTCTGACGAG 
TCTCGACTGT CAAACCTTCT TCGGAGGATT ACCCGGGAAG ACGACAGAGA CCGAAGACTG 
GCTACTGTGA AGCAATTGAA AGAATTCATT CAGCAACCAG AAAATAAGCA GGTCTTAGTT 
AAACAACTGG ATAATATCCT GACCGCTATA CATGATGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAAT TGAGGCAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTTTGAGT 
TATGAGGCCG AGAAGATCTT CAAATGGATT TTTAGCAAAT TTAGCTCGTC CACAAAAGAT 
GAAGTTAAAC TGCTTTACTT ATGTGCCACC TTCAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCCAT TCTAGAAAAT 
GTGGATACAC CAGAATTGCT ATGCAAATGT GTGAAGTGCA TTCTTCTGGT GGCCCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACGGTTG ACATATTGGT TGGGTGGCAT 
ATAGATCACA CTCAGAAACC TTCCTTGACA CAGCAAGTGT CTGGATGGCT GCAGAGTCTG 
GAGCCCTTTT GGGTAGCCGA TCTTGCATTT TCCACCACCC TTCTCGGTCA GTTTCTGGAA 
GATATGGAGG CTTATGCTGA AGATCTCAGT CATGTGGCAT CTGGGGAATC GGTCGATGAA 
GACATTCCTC CTCCATCAGT GTCCTTACCG AAGTTGGCGG CTCTTCTCCG GGTTTTCAGT 
ACTGTGGTGC GGAGTATTGG GGAGCGCTTC AGTCCAATTA GAGGACATCC AATTACCGAG 
GCTTATGTAA CAGATGTTTT GTACAGAGTA ATGAGATGTG TGACAACAGC AAACCAAGTA 
TTTTTTTCTG AAGCTGTATT GACAGCTGCC AATGAATGTG TCGGTGTATT GCTTGGTAGC 
CTAGATCCTA GTATGACTAT TCATTGTGAC ATGGTCATCA CCTATGGACT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACAGATTAT ATCATCTCGG TCTTAAATTT ACTGACGTTG 
ATTGTTGAGC AGATCAATAC AAAGCTGCCA TCGTCATTCG TCGAAAAACT GTTTATACCA 
GCGTCTAAAC TACTGCTTCT GCGCTATCAT AAAGAAAAAG AGGTTGTTGC AGTAGCTCAT 
GCTGTATATC AAGCCGTTCT TAGCCTGAAG AACATTCCCG TCTTGGAGAC CGCCTATAAG 
TTAATTTTAG GAGAAATGAC TTGTGCACTG AATAATCTCC TGCATAACCT TCAACTTCCT 
GAGGCCTGTT CCGAAATCCA ACACGATGCT TTTAAGAATC ACGTTTTCAA TGTGGACAAT 
GCCAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACCA CGATTGGAAA TGCGAAAAAC 
TCATTAATTG GGATGTGGGC ACTCTCGCCA ACCGTTTTTG CACTTCTGAG CAAGAATCTG 
ATGATAGTGC ATGGAGATCT CGCCGTTCAC TTCCCAGCTA TTCAGTATGC CGTTCTCTAC 
ACTTTGTATT CACATTGTAC CAGACATGAT CACTTCATTT CTAGCAGTCT TAGCTCCTCT 
TCTCCTTCTC TCTTTGATGG AGCAGTTATC AGCACTGTCA CCACAGCTAC AAAGAAGCAT 
TTCTCAATCA TATTAAACCT CCTGGGAATA TTACTGAAGA AAGATAATCT TAATCAGGAT 
ACCAGGAAAC TACTAATGAC CTGGGCTTTG GAAGTTGCTG TTCTAATGAA AAAGTCTGAA 
ACATATGCAC CTTTGTTCTC TCTTCCGTCG TTCCATAAAT TTTGCAAAGG ACTTCTAGCA 
AACACTCTTC TTGAAGATGT GAATATCTGC CTTCAAGCAT GTAGTAGTCT GCATGCCTTG 
TCATCTTCCC TGCCAGATGA CCTTTTACAA AGGTGTGTTG ATGTGTGCCG CGTTCAGCTG 
GTCCATAGTG GAACTCGGAT TCGACAAGCC TTTGGAAAGC TGTTGAAGTC TATTCCGTTA 
GATGTTGTCT TGAGCAATAG CAACCACACA GAGATTCAAG AAATTTCCTT AGCTATAAGG 
AGTCACATGA GCAAAACTCC AAGTAATACT TTCCATCCAC AAGATTTCTC TGACGTGATA 
AGTTTTATTT TGTATGGAAA CTCTCATAGA ACCGGGAAGG ACAATTGGTT AGAAAGATTG 
TTCTACAGCT GTCAGAGATT GGATAAGCGT GATCAGTCAA CCATTCCACG CAATCTACTC 
AAAACAGATG CCGTCCTCTG GCAGTGGGCT ATTTGGGAAG CTGCTCAGTT CACTGTTCTC 
TCTAAACTAA GAACCCCGCT GGGCAGAGCT CAAGATACCT TCCAGACAAT CGAAGGTATC 
ATTAGAAGTC TTGCCGCTCA TACATTAAAC CCCGATCAAG ATGTTAGCCA GTGGACCACT 
GCGGACAATG ATGAAGGACA TAGCAGCAAC CAACTCAGAC TTGTTCTTCT ACTGCAGTAC 
TTGGAAAATT TGGAAAAACT AATGTATAAT GCATATGAAG GTTGTGCTAA TGCACTGACT 
TCCCCTCCTA AGGTTATCAG GACATTTTTC TATACCAATC GCCAGACATG TCAGGACTGG 
TTAACTCGGA TTAGACTTTC CATCATGAGA GTTGGATTGT TGGCCGGTCA GCCCGCTGTG 
ACAGTAAGAC ACGGTTTTGA TTTGCTCACC GAAATGAAGA CCAATAGTAT CTCTCAGGGA 
AATGAGCTAG AAGTGATTAT TATGATGGTC GTAGAAGCAC TTTGTGAGCT TCACTGTCCT 
GAAGCTATAC AGGGATTAGC TGTCTGGTCT TCAGCAGTGG TTGGAAAGAG TCTCGTCTGG 
ATTAATTCAG TGGCACAGCA AGCCGAAGGG CGGTTTGAAA AGGCAACTGT TGAATACCAG 
GAGCACTTGT GTGCTATGAC AGGGGTAGAT TGCTGCATAA CTGGGTTTGA TAAATCCATT 
CTGAAGTTGG CTAACATGAA TACTAACAAT ACCAGCCCCA AACATTCTCT CAATGGTGAC 
GCTAGGAAAA CTGTTCTGGC CAAACCAAGT GATTCTTCTC CTGAGGTTAT TAATTACTTG 
GGTAACAAAG CATGTGAGTG CTACATCTCT CTTGCTGATT GGGCTGCTGT GCAGGAATGG 
CAGAATGCTG TCCATGACTT GAAAAAGAGC ACAAGTAGTA CTTCCCTCAA TTTAAAAGCT 
GACTTTAACT ACATAAAATC ACTGAGCAGC TTTGAATCTG GCGAATTTCT CGAATGTACA 
GAACAACTAG AATTGCTGCC AGGAGAAAAC ATTAACTTAC TTGCCAGCGG GTCAAAGGAA 
AAAATAGATA TGAAAAAGCT ACTTCCAAAC ATGTTAAGCC CAGATCCACG GGAACTTCAG 
AAATCCATTG AAGTTCAGTT GCTGAGAAGT TCCGTTTGTT TGGCTACCGC TGTAAGCCAC 
ATAGAGCAGG AACAAAAGTG GCAATCTATA ACTGAAAACG TTGTAAAATA TTTGAAGCAA 
ACATCAAGAA TAGCTATTGG ACCACTGAGA CTTTCCACTT TAACTGTTTC ACAGTCCTTA 
CCAGTTCTAA GTACCCTACA GCTGTATTGT TCTTCTGCTT TGGAGAACAC CGTGTCAAAC 
AGGCTTTCGA CAGAGGACTG CTTAATCCCA CTCTATAGTG AAGCTTTACG TTCGTGTAAA 
CAGCATGATG TAAGGCCCTG GATGCAGGCT TTGAGGTATA CAGTATACCA GAGTCAGTTG 
GTGGAAAAAA TCAAAGAACA AACGGTTCCA ATTAGAAGTC ACCTCATGGA ATTGGGTTTA 
ACAGCTGCGA AATTTGCTCG TAAACGTGGA AACGTTGCCC TGGCAACCAG ATTGCTCGCT 
CAATGTAGTG AAGTGCGGTT GGGAAAGACG ACCACTGCTC AAGATTTAGT CCAGTATTTT 
AAAAAACTGT CAACACAAGG TCAAATAGAT GAAAAATGGG GACCTGAACT GGATATTGAG 
AAAACGAAAT TGTTATACAC AGCAGGTCAG TCAACCCATG CGATGGAAAT GCTGAGTTCT 
TGCGCCATAT CCTTCTGCAA GTCTGCCAAA GCTGAATACG CAGTAGCCAA ATCGATTCTT 
ACACTTGCTA AATGGATCCA AGCTGAATGG AAAGAAATTT CAGGACAGTT GAAACAGGTG 
TACAGAGCCC AACAGCAGCA GAACACCACG GGCCTGTCTA CATTATCTAA AAACATACAC 
ACTTTGATCG AACTGCCGTC TGTCAACACC ACAGAAGAGG AGTATCCCCG CATCGAGAGT 
GAATCAACAG TCAACATTGG AGTTGGGGAA CCGGACTTCA TCCTGGGCCA GTTGTATCAC 
CTGTCTTCAG TGCAGGCACC CGAAGTGGCC AAATCTTGGG CAGCGCTGGC AAGTTGGGCT 
TACAGATGGG GAAGAAAGGT GGTGGATAAC GCCAGTCAGG GAGAAGGTGT TCGACTACTG 
CCAAGAGAGA AATCTGAGGT TCAAAATCTG CTTCCAGACA ACATAACAGA GGAGGATCGA 
GAGAAGATCT ACGGCATCCT TGGGCAGGCG GTGTGTCGGC CAGCCGGAAT TCAGGATGAA 
GATATAACAC TGCAGATAAC AGAGAGTGAA GACAATGAGG AAGATGACAT GGTGGATGTG 
ATCTGGCGTC AGTTATTGAC GAGTTGCCCG TGGCTTTCCG ATCTTGATGA AAATGCCACA 
GAAGGCCTAA TTAAAGTGTG GAGAAAAATT GTAGATAGGA TATTCAGTCT CTACAAACTG 
TCCTGCAGTG CCTATTTTAC TTTCCTTAAA CTCAATGCTG GCCAGGTTAC TCTAGATGAA 
GATGACCCAA GGCTGCACTT GAGCATTAGG TCTGAACAAA GCACTGACGA TGTTATTGTA 
ATGGCAACGT TAAGACTGTT GAGATTGCTG GTCAAACACG CTGGAGAGCT AAGGCAGTAT 
CTGGAGCACG GCCTAGAAAC CACCCCCACT GCTCCTTGGA GAGGTATCAT TCCACAGCTC 
TTCTCCCGTC TAAATCATCC CGAAGTGTAC GTCCGCCAAA GTATTTGTAA TCTACTCTGT 
CGAGTGGCAC AAGATTCTCC TCATCTCATA TTATATCCTG CAATTGTGGG AACTATTTCA 
CTGAGTAGTG AATCGCAGAC ATCAGGCAAT AAGTTGCCTT CAGCCATCCC CACTTTGCTG 
GGCAATATTC AAGGCGAAGA ATTACTCGGA GCGGAATGTG AAGGGGGAAG TCCTCCAGCT 
TCTCAAGACA GCAATAAAGA TGAACCCAAG AATGGCTTAA ATGAAGATCA AGCGATGATG 
CAGGATTGCT ACAGCAAAAT TGTTGATAAA TTGTCTTCTG CCAACCCAAC AATGGTCTTA 
CAGGTTCAAA TGCTGGTGGC CGAGTTGCGC AGGGTGACCG TTCTTTGGGA TGAGCTCTGG 
CTGGGAGTTC TTCTACAACA ACATATGTAT GTTCTGAGGC GCATTCAACA GTTGGAGGAC 
GAAGTAAAGA GAGTCCAGAA TAACAATACG TTACGCAAAG AAGAGAAGAT TGCAATAATG 
CGAGAAAAGC ACACAGCTCT GATGAAGCCC ATCGTATTTG CTTTGGAACA TGTACGGAGC 
ATCACTGCCG CTCCTGCCGA AACACCTCAT GAGAAGTGGT TCCAAGACAA CTATGGTGAG 
GCAATTGAAA ATGCCCTGGA AAAACTCAAG AACCCATTAA ATCCTGCAAA ACCCGGAAGC 
AGTTGGATCC CATTTAAAGA GGTGATGCTA AGTTTGCAGC AGAGAGCACA GAAACGTGCT 
AGTTATATCT TGCGCCTTGA AGAAATCAGC CCCTGGTTGG CTGCCATGAC TAATACTGAA 
ATTGCCCTTC CTGGAGAAGT ATCAGCCAGA GATACCGTGA CGATCCACAG CGTGGGCGGA 
ACCATCACAA TCCTACCTAC TAAAACCAAA CCCAAAAAAC TGCTCTTTCT GGGTTCAGAC 
GGGAAAAACT ACCCCTATCT CTTTAAAGGA CTGGAAGATC TGCACCTGGA TGAGAGAATC 
ATGCAGTTTC TATCTATCGT TAATACCATG TTCGCTACCA TCAATCGCCA AGAGACGCCC 
CGTTTCCATG CCAGGCACTA TTCGGTGACC CCGCTGGGGA CGAGGTCGGG CCTGATTCAG 
TGGGTAGATG GAGCCACTCC CTTATTTGGC CTTTACAAGC GATGGCAACA GCGAGAAGCT 
GCTTTACAAG CGCAAAAGGC CCAGGATTCT TACCAGACTC CTCAAAATCC TGGAATAGTA 
CCTCGGCCCA GCGAGCTTTA TTACAGTAAA ATCGGGCCCG CCCTGAAAGC GGTCGGACTC 
AGTCTCGACG TGTCCCGGCG AGACTGGCCT CTCAACGTGA TGAAGGCTGT GTTGGAAGAA 
CTGATGGAAG CCACCCCTCC AAACCTTCTG GCCAAGGAGC TTTGGTCTTC TTGTACCACA 
CCTGACGAAT GGTGGAGAGT TACCCAGTCT TACGCAAGAT CCACCGCGGT CATGTCAATG 
GTTGGATACA TAATCGGCCT TGGAGATAGA CATCTGGATA ACGTTCTTAT AGACATGACT 
ACAGGAGAAG TTGTTCACAT AGATTACAAT GTTTGTTTTG AAAAAGGTAA AAGTCTGAGA 
GTGCCGGAGA AAGTTCCTTT CAGGATGACT CACAACATCG AAACAGCTCT CGGTGTAACT 
GGAGTTGAGG GAGTTTTCAG GCTTTCTTGT GAACAGGTTC TCCAAATTAT GCGGCGTGGA 
AGAGAGACCC TGCTCACGTT GCTGGAAGCC TTTGTGTACG ATCCTCTTGT GGACTGGACA 
GCCGGAGGGG AAGCAGGATT TGCAGGAGCT GTCTATGGCG GAGGCGGCCA GCAGGCAGAG 
AACAAGCAAA GTAAAAGGGA GATGGAAAGG GACATCACCC GCAGCCTCTT TTCTTCCCGA 
GTAGCTGAAA TCAAGGTGAA CTGGTTTAAA AACAGAGATG AGATGTTGAT TGTGCTTCCA 
AAGCTAGATG GAAGCTTAGA AGAATACCTG AGCCTACAAG AGCAATTGAC AGATGTTGAA 
AAACTGCAAG GCAAACTCGT TGAGGAGATT GACTTTCTAG AAGGAGCCGA AGGAGTAGAT 
CATCCCTCGC ACACTCTGCA GCACAGGTAT TCCGAACACA CCCAACTGCA GACTCAGCAG 
AGAACCGTTC AAGAAGCCAT CCAGGTGAAG TTGAATGAAT TTGAGCAGTG GATAACACAT 
TACCAAGCTG CGTTTAACAA TTTAGAAGCC ACCCAGCTGG CCAGTCTGCT TCAGGAGATT 
AGCACCCAAA TGGACCTGGG TCCTCCAAGC TACGTGCCTG CAACCACCTT TCTGCAGAAC 
GCTGGCCAAG CCCACTTGAT CAGTCAGTGC GAGCAGCTGG AGGGCGAGGT TGGAGCTTTC 
CTTCAGCAGA GGCGCTCCGT CCTGCGAGGC TGTCTCGAGC AGCTGCATAA CTACGCGACC 
GTAGCCCTTC AGTACCCGAA AACCATATTC CAGAAACACC GAATCGAGCA GTGGAAGACC 
TGGATGGAAG AACTTATACG CAATACGACC ATCGAGAGAT GTCAGGAGTT GTACCGGAAA 
TATGAACTCC AGTATGCCCC CCAGCCACCA CCCACGGTGT GTCAGTTCAT AACTGCCACT 
GAAATGACTC TTCAGCGCTA CGCGGCAGAC ATAAATAATA GGCTTATCAG ACAAGTGGAG 
CGCCTGAAAC AAGAAGCAGT GACCGTGCTG GTCTGCGAGG AGCAGTTAAA AGAAATTGAA 
CGGTGTATTA AGGTTTTTCT TCATGAAAAC GGAGAGGAGG GGTCCTGGAG CCTGGCGAGC 
GTTATCATCT CTGCACTCTG TGCACTTACA AGGCGCAACT TAATGATGGA AGGTGCCGCT 
TCTAGTGCTG GCGAGCAGCT GGTCGATCTG ACTTCCCGAG ATGGGGCCTG GTTCTTGGAG 
GAGCTGTGCA GCATGAGTGG AAATGTAACC TGCCTTGTAC AGTTACTGAA GCAGTGTCAC 
CTGGTCCCTC AGGATTTAGA TATTCCCAAT CCCCTCGAAG CTTCGGAGGC AGTTCACTTA 
GCTAATGGAG TCTACACCTC GCTTCAGGAA TTGAACTCCA ACTTCCGGCA GATCATCTTT 
CCGGAAGCTC TGCGATGTTT AATGAAAGGG GAATACACTT TAGAAAGCAT GCTGCACGAA 
CTGGACAACC TCATTGAACA GACAACTGAT GGAGTTCCGT TGCAAACCCT GGTTGACTCT 
CTTCAGGCCT ATTTAAGAAA TGCAGCTATG GGCCTGGAAG AAGAGACACA TTCTCACTAT 
ATTGATGTTG CCAGAATACT CCATTCTCAG TATGGCGAAT TGATTCAACC AAGGAATGGT 
TCAGTTGATG AAACACCAAA AATGTCCGCC GGTCAAATGC TTTTGGTTGC GTTCGATGGA 
ATGTTCGCCC AAGTGGAAAC CGCTTTTGGC TTGTTAATTG AAAAGTTAAA CAAGATGGAA 
GTCCCCGTAT CGTGGCGAAA GATAGACATC ATAAGAGAAG CCAGAAGCAC CCAGATTAAT 
TTCTTTGACG ACGATAATCA CCGACAGGTT CTGGAGGAGA TTTTCTTTCT CAAAAGACTG 
CAGACCATCA AAGAATTCTT TAGACTCTGT GGTACTTTTT CTAAAACGCT GTCAGGATCG 
AGTTCGCTCG AAGATCAGAG CCCGGTGAAT GGACCCGTTC AAATTGTTAA TGTGAAAACG 
CTTTTCAGAA ACTCTTGTTT TAGTGAAGAT CAGATGGCCA AACCCATAAA GGCGTTCACA 
GCTGACTTCG TGAGGCAGCT CTTAATTGGC CTGCCTAATC AAGCCTTGGG GCTAACCCTT 
TGCAGTTTCA TCAGCGCGCT GGGCGTCGAC ATCATCGCCC AGGTCGAGGC AAAGGACTTT 
GGGGCAGAGA GCAAGGTTTC GGTGGACGAT CTGTGTAAGA AAGCCGTGGA GCACAACATC 
CAGATTGGAA AGTTCTCCCA GCTGGTCATG AACCGAGCCA CGGTCCTGGC CAGTTCCTAC 
GACACTGCTT GGAAGAAGCA CGATTTGGTG CGCAGGCTAG AAACCAGTAT TTCCTCTTTC 
AAGACCAGCC TGCAGAGAGT GCAGCTCCAG ATCGCCATGT TTCAGTGGCA GCACGAAGAT 
CTCCTAATCA CCCGACCACA AGCCATGTCT GTGACTCCTC CACCTCGCTC TGCTATTCTA 
ACAAATATGA AAAAGAAGCT GCATACCCTG AGCCAGATCG AAACGTCCAT TGCAGCGGTT 
CAGGAGAAAC TAGCAGCCCT CGAAGCAAGC ATCGAACAAC GTTTGAAGTG GGCAGGAGGG 
GCCAATCCCG CATTGGCTCC AGTCCTACAA GATTTTGAGG CAACAATAGC GGAAAGAAGG 
AATCTCGTTC TGAAAGAAAG CCAACGAGCA AATCAGGTCA CTTTCCTCTG CAGCAATATC 
ATTCATTTTG AAAGCCTACG AACGCGAACT GCCGAAGCTC TAAATCTCGA TGCTGCCTTG 
TTTGAACTCA TCAAGCGCTG TCAACAGATG TGTTCGTTTG CCTCACAGTT TAACAGCTCG 
GTTTCTGAAC TGGAACTGCG TTTACTGCAA AGAGTGGGTT CCGGTCTCGA ACCTCCCATT 
GGCAGCCCTG AATGGCTTGT TTCTGCACAC AAACAACTGA CCCAGGATAT GTCTACTCAG 
AGGGCGGTTC AAACAGAAAA AGAACAGCAA ATCGAATCAG TCTGTGAAGC AATTCAGAAC 
CTTGTGGATA GTATTAAGAC AGTGCTTACC GGTCACAACA GGCAACTTGG AGATGTTAAA 
CATCTATTGA AAGCAATGGC CAAGGATGAA GAGGCTGCTT TGGCAGATGG TGAAGATGTT 
CCCTATGAGA ACAGTGTTAG ACAATTCTTG GGTGAATATA AGTCATGGCA AGACAATATT 
CAAACGGTAT TGTTTACATT AGTTCAGGCT ATGGGGCAGG TTCGCAGCCA AGAGCATGTG 
GAGATGCTGC AGGAGATAAC TCCCACCTTG AAAGAGCTGA AAACACAAAG CCAGAGTATC 
TACAATAATC TAGTGAGTTT TGCATCACCC TTAGTCACAG ATGCAACAAA TGAGTGTTCC 
AGCCCAACAT CATCTGCTAC GTATCAGCCA ACCTTTGCTG CAGCGGTCCG CAGTAACACT 
GGCCAGAAGA CTCAGCCTGA AGTTATGTCG CAGAATGCAA GGAAGCTGAT TCAGAAAAAT 
CTTGCAACAT CAGCAGATAC CCCACCAAGC ACAGTTCCAG GAACTGGCAA GAGTGTGGCC 
TGTAGTCCTA AAAAAGCAGT CAGGGATCCA AAAACTGGAA AAGCTGTGCA AGAAAGGAAC 
TCCTATGCAG TGAGTGTGTG GAAGCGAGTC AAAGCCAAGT TAGAGGGCCG AGATGTGGAT 
CCGAACAGGA GGATGTCTGT TGCTGAACAG GTGGACTATG TGATTAAGGA AGCAACTAAT 
CTAGATAACT TGGCTCAGCT GTATGAAGGT TGGACGGCCT GGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_029058053.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028913886.1 Ornithorhynchus anatinus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

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CloneID	OOc122577
Clone ID Related Accession (Same CDS sequence)	XM_029058053.1
Accession Version	XM_029058053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCGGCGGCG GCACCAAGTA CCCGCGGAGC 
TGGAATGACT GGCCACCCAG AACTGATAGT GCACCAGCCG ACCAGGATCA TTTAAAATAT 
TCTTCGTCTA GAGATCGGGG CAGTTCTGCC TCTTATGGAT TACAACCTTC AAATTCAGTT 
GTGGTGTCTC GTCAACGGCA CGATGATATC AGAGTCCACA CCGACATACA GAATGAAGAA 
AAGGGTGGCT ACAATGTCAA TGGAGGAGCT GGGGAAAATA CTTATGGTCG GAAGTCGTTG 
GGGCAAGAGC TGAGGGTTAA CAATGTGACC AACCCTGATT TCACCAGTGT TCAGCATGGA 
AATCGTGCTT TAGCCACAAA AGACATGAGG AAATCACAGG AGCGATCGTT GTCTTACTCT 
GACGAGTCTC GACTGTCAAA CCTTCTTCGG AGGATTACCC GGGAAGACGA CAGAGACCGA 
AGACTGGCTA CTGTGAAGCA ATTGAAAGAA TTCATTCAGC AACCAGAAAA TAAGCAGGTC 
TTAGTTAAAC AACTGGATAA TATCCTGACC GCTATACATG ATGTGCTTAA TGAAAGTAGC 
AAATTGCTTC AGGAATTGAG GCAGGAGGGA GCTTGCTGTC TTGGCCTTCT TTGTGCTTCT 
TTGAGTTATG AGGCCGAGAA GATCTTCAAA TGGATTTTTA GCAAATTTAG CTCGTCCACA 
AAAGATGAAG TTAAACTGCT TTACTTATGT GCCACCTTCA AAGCACTAGA GACTGTAGGA 
GAAAAGAAAG CCTTTTCATC TGTAATGCAG CTTGTAATGA CCAGCCTGCA GTCCATTCTA 
GAAAATGTGG ATACACCAGA ATTGCTATGC AAATGTGTGA AGTGCATTCT TCTGGTGGCC 
CGATGTTACC CTCATATTTT CAGCACTAAT TTTAGGGATA CGGTTGACAT ATTGGTTGGG 
TGGCATATAG ATCACACTCA GAAACCTTCC TTGACACAGC AAGTGTCTGG ATGGCTGCAG 
AGTCTGGAGC CCTTTTGGGT AGCCGATCTT GCATTTTCCA CCACCCTTCT CGGTCAGTTT 
CTGGAAGATA TGGAGGCTTA TGCTGAAGAT CTCAGTCATG TGGCATCTGG GGAATCGGTC 
GATGAAGACA TTCCTCCTCC ATCAGTGTCC TTACCGAAGT TGGCGGCTCT TCTCCGGGTT 
TTCAGTACTG TGGTGCGGAG TATTGGGGAG CGCTTCAGTC CAATTAGAGG ACATCCAATT 
ACCGAGGCTT ATGTAACAGA TGTTTTGTAC AGAGTAATGA GATGTGTGAC AACAGCAAAC 
CAAGTATTTT TTTCTGAAGC TGTATTGACA GCTGCCAATG AATGTGTCGG TGTATTGCTT 
GGTAGCCTAG ATCCTAGTAT GACTATTCAT TGTGACATGG TCATCACCTA TGGACTAGAC 
CAACTGGAGA ATTGCCAGAC TTGTGGTACA GATTATATCA TCTCGGTCTT AAATTTACTG 
ACGTTGATTG TTGAGCAGAT CAATACAAAG CTGCCATCGT CATTCGTCGA AAAACTGTTT 
ATACCAGCGT CTAAACTACT GCTTCTGCGC TATCATAAAG AAAAAGAGGT TGTTGCAGTA 
GCTCATGCTG TATATCAAGC CGTTCTTAGC CTGAAGAACA TTCCCGTCTT GGAGACCGCC 
TATAAGTTAA TTTTAGGAGA AATGACTTGT GCACTGAATA ATCTCCTGCA TAACCTTCAA 
CTTCCTGAGG CCTGTTCCGA AATCCAACAC GATGCTTTTA AGAATCACGT TTTCAATGTG 
GACAATGCCA AATTTGTAGT TATATTTGAC CTCAGTGCCC TGACCACGAT TGGAAATGCG 
AAAAACTCAT TAATTGGGAT GTGGGCACTC TCGCCAACCG TTTTTGCACT TCTGAGCAAG 
AATCTGATGA TAGTGCATGG AGATCTCGCC GTTCACTTCC CAGCTATTCA GTATGCCGTT 
CTCTACACTT TGTATTCACA TTGTACCAGA CATGATCACT TCATTTCTAG CAGTCTTAGC 
TCCTCTTCTC CTTCTCTCTT TGATGGAGCA GTTATCAGCA CTGTCACCAC AGCTACAAAG 
AAGCATTTCT CAATCATATT AAACCTCCTG GGAATATTAC TGAAGAAAGA TAATCTTAAT 
CAGGATACCA GGAAACTACT AATGACCTGG GCTTTGGAAG TTGCTGTTCT AATGAAAAAG 
TCTGAAACAT ATGCACCTTT GTTCTCTCTT CCGTCGTTCC ATAAATTTTG CAAAGGACTT 
CTAGCAAACA CTCTTCTTGA AGATGTGAAT ATCTGCCTTC AAGCATGTAG TAGTCTGCAT 
GCCTTGTCAT CTTCCCTGCC AGATGACCTT TTACAAAGGT GTGTTGATGT GTGCCGCGTT 
CAGCTGGTCC ATAGTGGAAC TCGGATTCGA CAAGCCTTTG GAAAGCTGTT GAAGTCTATT 
CCGTTAGATG TTGTCTTGAG CAATAGCAAC CACACAGAGA TTCAAGAAAT TTCCTTAGCT 
ATAAGGAGTC ACATGAGCAA AACTCCAAGT AATACTTTCC ATCCACAAGA TTTCTCTGAC 
GTGATAAGTT TTATTTTGTA TGGAAACTCT CATAGAACCG GGAAGGACAA TTGGTTAGAA 
AGATTGTTCT ACAGCTGTCA GAGATTGGAT AAGCGTGATC AGTCAACCAT TCCACGCAAT 
CTACTCAAAA CAGATGCCGT CCTCTGGCAG TGGGCTATTT GGGAAGCTGC TCAGTTCACT 
GTTCTCTCTA AACTAAGAAC CCCGCTGGGC AGAGCTCAAG ATACCTTCCA GACAATCGAA 
GGTATCATTA GAAGTCTTGC CGCTCATACA TTAAACCCCG ATCAAGATGT TAGCCAGTGG 
ACCACTGCGG ACAATGATGA AGGACATAGC AGCAACCAAC TCAGACTTGT TCTTCTACTG 
CAGTACTTGG AAAATTTGGA AAAACTAATG TATAATGCAT ATGAAGGTTG TGCTAATGCA 
CTGACTTCCC CTCCTAAGGT TATCAGGACA TTTTTCTATA CCAATCGCCA GACATGTCAG 
GACTGGTTAA CTCGGATTAG ACTTTCCATC ATGAGAGTTG GATTGTTGGC CGGTCAGCCC 
GCTGTGACAG TAAGACACGG TTTTGATTTG CTCACCGAAA TGAAGACCAA TAGTATCTCT 
CAGGGAAATG AGCTAGAAGT GATTATTATG ATGGTCGTAG AAGCACTTTG TGAGCTTCAC 
TGTCCTGAAG CTATACAGGG ATTAGCTGTC TGGTCTTCAG CAGTGGTTGG AAAGAGTCTC 
GTCTGGATTA ATTCAGTGGC ACAGCAAGCC GAAGGGCGGT TTGAAAAGGC AACTGTTGAA 
TACCAGGAGC ACTTGTGTGC TATGACAGGG GTAGATTGCT GCATAACTGG GTTTGATAAA 
TCCATTCTGA AGTTGGCTAA CATGAATACT AACAATACCA GCCCCAAACA TTCTCTCAAT 
GGTGACGCTA GGAAAACTGT TCTGGCCAAA CCAAGTGATT CTTCTCCTGA GGTTATTAAT 
TACTTGGGTA ACAAAGCATG TGAGTGCTAC ATCTCTCTTG CTGATTGGGC TGCTGTGCAG 
GAATGGCAGA ATGCTGTCCA TGACTTGAAA AAGAGCACAA GTAGTACTTC CCTCAATTTA 
AAAGCTGACT TTAACTACAT AAAATCACTG AGCAGCTTTG AATCTGGCGA ATTTCTCGAA 
TGTACAGAAC AACTAGAATT GCTGCCAGGA GAAAACATTA ACTTACTTGC CAGCGGGTCA 
AAGGAAAAAA TAGATATGAA AAAGCTACTT CCAAACATGT TAAGCCCAGA TCCACGGGAA 
CTTCAGAAAT CCATTGAAGT TCAGTTGCTG AGAAGTTCCG TTTGTTTGGC TACCGCTGTA 
AGCCACATAG AGCAGGAACA AAAGTGGCAA TCTATAACTG AAAACGTTGT AAAATATTTG 
AAGCAAACAT CAAGAATAGC TATTGGACCA CTGAGACTTT CCACTTTAAC TGTTTCACAG 
TCCTTACCAG TTCTAAGTAC CCTACAGCTG TATTGTTCTT CTGCTTTGGA GAACACCGTG 
TCAAACAGGC TTTCGACAGA GGACTGCTTA ATCCCACTCT ATAGTGAAGC TTTACGTTCG 
TGTAAACAGC ATGATGTAAG GCCCTGGATG CAGGCTTTGA GGTATACAGT ATACCAGAGT 
CAGTTGGTGG AAAAAATCAA AGAACAAACG GTTCCAATTA GAAGTCACCT CATGGAATTG 
GGTTTAACAG CTGCGAAATT TGCTCGTAAA CGTGGAAACG TTGCCCTGGC AACCAGATTG 
CTCGCTCAAT GTAGTGAAGT GCGGTTGGGA AAGACGACCA CTGCTCAAGA TTTAGTCCAG 
TATTTTAAAA AACTGTCAAC ACAAGGTCAA ATAGATGAAA AATGGGGACC TGAACTGGAT 
ATTGAGAAAA CGAAATTGTT ATACACAGCA GGTCAGTCAA CCCATGCGAT GGAAATGCTG 
AGTTCTTGCG CCATATCCTT CTGCAAGTCT GCCAAAGCTG AATACGCAGT AGCCAAATCG 
ATTCTTACAC TTGCTAAATG GATCCAAGCT GAATGGAAAG AAATTTCAGG ACAGTTGAAA 
CAGGTGTACA GAGCCCAACA GCAGCAGAAC ACCACGGGCC TGTCTACATT ATCTAAAAAC 
ATACACACTT TGATCGAACT GCCGTCTGTC AACACCACAG AAGAGGAGTA TCCCCGCATC 
GAGAGTGAAT CAACAGTCAA CATTGGAGTT GGGGAACCGG ACTTCATCCT GGGCCAGTTG 
TATCACCTGT CTTCAGTGCA GGCACCCGAA GTGGCCAAAT CTTGGGCAGC GCTGGCAAGT 
TGGGCTTACA GATGGGGAAG AAAGGTGGTG GATAACGCCA GTCAGGGAGA AGGTGTTCGA 
CTACTGCCAA GAGAGAAATC TGAGGTTCAA AATCTGCTTC CAGACAACAT AACAGAGGAG 
GATCGAGAGA AGATCTACGG CATCCTTGGG CAGGCGGTGT GTCGGCCAGC CGGAATTCAG 
GATGAAGATA TAACACTGCA GATAACAGAG AGTGAAGACA ATGAGGAAGA TGACATGGTG 
GATGTGATCT GGCGTCAGTT ATTGACGAGT TGCCCGTGGC TTTCCGATCT TGATGAAAAT 
GCCACAGAAG GCCTAATTAA AGTGTGGAGA AAAATTGTAG ATAGGATCTT CAGTCTCTAC 
AAACTGTCCT GCAGTGCCTA TTTTACTTTC CTTAAACTCA ATGCTGGCCA GGTTACTCTA 
GATGAAGATG ACCCAAGGCT GCACTTGAGC ATTAGGTCTG AACAAAGCAC TGACGATGTT 
ATTGTAATGG CAACGTTAAG ACTGTTGAGA TTGCTGGTCA AACACGCTGG AGAGCTAAGG 
CAGTATCTGG AGCACGGCCT AGAAACCACC CCCACTGCTC CTTGGAGAGG TATCATTCCA 
CAGCTCTTCT CCCGTCTAAA TCATCCCGAA GTGTACGTCC GCCAAAGTAT TTGTAATCTA 
CTCTGTCGAG TGGCACAAGA TTCTCCTCAT CTCATATTAT ATCCTGCAAT TGTGGGAACT 
ATTTCACTGA GTAGTGAATC GCAGACATCA GGCAATAAGT TGCCTTCAGC CATCCCCACT 
TTGCTGGGCA ATATTCAAGG CGAAGAATTA CTCGGAGCGG AATGTGAAGG GGGAAGTCCT 
CCAGCTTCTC AAGACAGCAA TAAAGATGAA CCCAAGAATG GCTTAAATGA AGATCAAGCG 
ATGATGCAGG ATTGCTACAG CAAAATTGTT GATAAATTGT CTTCTGCCAA CCCAACAATG 
GTCTTACAGG TTCAAATGCT GGTGGCCGAG TTGCGCAGGG TGACCGTTCT TTGGGATGAG 
CTCTGGCTGG GAGTTCTTCT ACAACAACAT ATGTATGTTC TGAGGCGCAT TCAACAGTTG 
GAGGACGAAG TAAAGAGAGT CCAGAATAAC AATACGTTAC GCAAAGAAGA GAAGATTGCA 
ATAATGCGAG AAAAGCACAC AGCTCTGATG AAGCCCATCG TATTTGCTTT GGAACATGTA 
CGGAGCATCA CTGCCGCTCC TGCCGAAACA CCTCATGAGA AGTGGTTCCA AGACAACTAT 
GGTGAGGCAA TTGAAAATGC CCTGGAAAAA CTCAAGAACC CATTAAATCC TGCAAAACCC 
GGAAGCAGTT GGATCCCATT TAAAGAGGTG ATGCTAAGTT TGCAGCAGAG AGCACAGAAA 
CGTGCTAGTT ATATCTTGCG CCTTGAAGAA ATCAGCCCCT GGTTGGCTGC CATGACTAAT 
ACTGAAATTG CCCTTCCTGG AGAAGTATCA GCCAGAGATA CCGTGACGAT CCACAGCGTG 
GGCGGAACCA TCACAATCCT ACCTACTAAA ACCAAACCCA AAAAACTGCT CTTTCTGGGT 
TCAGACGGGA AAAACTACCC CTATCTCTTT AAAGGACTGG AAGATCTGCA CCTGGATGAG 
AGAATCATGC AGTTTCTATC TATCGTTAAT ACCATGTTCG CTACCATCAA TCGCCAAGAG 
ACGCCCCGTT TCCATGCCAG GCACTATTCG GTGACCCCGC TGGGGACGAG GTCGGGCCTG 
ATTCAGTGGG TAGATGGAGC CACTCCCTTA TTTGGCCTTT ACAAGCGATG GCAACAGCGA 
GAAGCTGCTT TACAAGCGCA AAAGGCCCAG GATTCTTACC AGACTCCTCA AAATCCTGGA 
ATAGTACCTC GGCCCAGCGA GCTTTATTAC AGTAAAATCG GGCCCGCCCT GAAAGCGGTC 
GGACTCAGTC TCGACGTGTC CCGGCGAGAC TGGCCTCTCA ACGTGATGAA GGCTGTGTTG 
GAAGAACTGA TGGAAGCCAC CCCTCCAAAC CTTCTGGCCA AGGAGCTTTG GTCTTCTTGT 
ACTACACCTG ACGAATGGTG GAGAGTTACC CAGTCTTACG CAAGATCCAC CGCGGTCATG 
TCAATGGTTG GATACATAAT CGGCCTTGGA GATAGACATC TGGATAACGT TCTTATAGAC 
ATGACTACAG GAGAAGTTGT TCACATAGAT TACAATGTTT GTTTTGAAAA AGGTAAAAGT 
CTGAGAGTGC CGGAGAAAGT TCCTTTCAGG ATGACTCACA ACATCGAAAC AGCTCTCGGT 
GTAACTGGAG TTGAGGGAGT TTTCAGGCTT TCTTGTGAAC AGGTTCTCCA AATTATGCGG 
CGTGGAAGAG AGACCCTGCT CACGTTGCTG GAAGCCTTTG TGTACGATCC TCTTGTGGAC 
TGGACAGCCG GAGGGGAAGC AGGATTTGCA GGAGCTGTCT ATGGCGGAGG CGGCCAGCAG 
GCAGAGAACA AGCAAAGTAA AAGGGAGATG GAAAGGGACA TCACCCGCAG CCTCTTTTCT 
TCCCGAGTAG CTGAAATCAA GGTGAACTGG TTTAAAAACA GAGATGAGAT GTTGATTGTG 
CTTCCAAAGC TAGATGGAAG CTTAGAAGAA TACCTGAGCC TACAAGAGCA ATTGACAGAT 
GTTGAAAAAC TGCAAGGCAA ACTCGTTGAG GAGATTGACT TTCTAGAAGG AGCCGAAGGA 
GTAGATCATC CCTCGCACAC TCTGCAGCAC AGGTATTCCG AACACACCCA ACTGCAGACT 
CAGCAGAGAA CCGTTCAAGA AGCCATCCAG GTGAAGTTGA ATGAATTTGA GCAGTGGATA 
ACACATTACC AAGCTGCGTT TAACAATTTA GAAGCCACCC AGCTGGCCAG TCTGCTTCAG 
GAGATTAGCA CCCAAATGGA CCTGGGTCCT CCAAGCTACG TGCCTGCAAC CACCTTCCTG 
CAGAACGCTG GCCAAGCCCA CTTGATCAGT CAGTGCGAGC AGCTGGAGGG CGAGGTTGGA 
GCTTTCCTTC AGCAGAGGCG CTCCGTCCTG CGAGGCTGTC TCGAGCAGCT GCATAACTAC 
GCGACCGTAG CCCTTCAGTA CCCGAAAACC ATATTCCAGA AACACCGAAT CGAGCAGTGG 
AAGACCTGGA TGGAAGAACT TATACGCAAT ACGACCATCG AGAGATGTCA GGAGTTGTAC 
CGGAAATATG AACTCCAGTA TGCCCCCCAG CCACCGCCCA CGGTGTGTCA GTTCATAACT 
GCCACTGAAA TGACTCTTCA GCGCTACGCG GCAGACATAA ATAATAGGCT TATCAGACAA 
GTGGAGCGCC TGAAACAAGA AGCAGTGACC GTGCTGGTCT GCGAGGAGCA GTTAAAAGAA 
ATTGAACGGT GTATTAAGGT TTTTCTTCAT GAAAACGGAG AGGAGGGGTC CTGGAGCCTG 
GCGAGCGTTA TCATCTCTGC ACTCTGTGCA CTTACAAGGC GCAACTTAAT GATGGAAGGT 
GCCGCTTCTA GTGCTGGCGA GCAGCTGGTC GATCTGACTT CCCGAGATGG GGCCTGGTTC 
TTGGAGGAGC TGTGCAGCAT GAGTGGAAAT GTAACCTGCC TTGTACAGTT ACTGAAGCAG 
TGTCACCTGG TCCCTCAGGA TTTAGATATT CCCAATCCCC TCGAAGCTTC GGAGGCAGTT 
CACTTAGCTA ATGGAGTCTA CACCTCGCTT CAGGAATTGA ACTCCAACTT CCGGCAGATC 
ATCTTTCCGG AAGCTCTGCG ATGTTTAATG AAAGGGGAAT ACACTTTAGA AAGCATGCTG 
CACGAACTGG ACAACCTCAT TGAACAGACA ACTGATGGAG TTCCGTTGCA AACCCTGGTT 
GACTCTCTTC AGGCCTATTT AAGAAATGCA GCTATGGGCC TGGAAGAAGA GACACATTCT 
CACTATATTG ATGTTGCCAG AATACTCCAT TCTCAGTATG GCGAATTGAT TCAACCAAGG 
AATGGTTCAG TTGATGAAAC ACCAAAAATG TCCGCCGGTC AAATGCTTTT GGTTGCGTTC 
GATGGAATGT TCGCCCAAGT GGAAACCGCT TTTGGCTTGT TAATTGAAAA GTTAAACAAG 
ATGGAAGTCC CCGTATCGTG GCGAAAGATA GACATCATAA GAGAAGCCAG AAGCACCCAG 
ATTAATTTCT TTGACGACGA TAATCACCGA CAGGTTCTGG AGGAGATTTT CTTTCTCAAA 
AGACTGCAGA CCATCAAAGA ATTCTTTAGA CTCTGTGGTA CTTTTTCTAA AACGCTGTCA 
GGATCGAGTT CGCTCGAAGA TCAGAGCCCG GTGAATGGAC CCGTTCAAAT TGTTAATGTG 
AAAACGCTTT TCAGAAACTC TTGTTTTAGT GAAGATCAGA TGGCCAAACC CATAAAGGCG 
TTCACAGCTG ACTTCGTGAG GCAGCTCTTA ATTGGCCTGC CTAATCAAGC CTTGGGGCTA 
ACCCTTTGCA GTTTCATCAG CGCGCTGGGC GTCGACATCA TCGCCCAGGT CGAGGCAAAG 
GACTTTGGGG CAGAGAGCAA GGTTTCGGTG GACGATCTGT GTAAGAAAGC CGTGGAGCAC 
AACATCCAGA TTGGAAAGTT CTCCCAGCTG GTCATGAACC GAGCCACGGT CCTGGCCAGT 
TCCTACGACA CTGCTTGGAA GAAGCACGAT TTGGTGCGCA GGCTAGAAAC CAGTATTTCC 
TCTTTCAAGA CCAGCCTGCA GAGAGTGCAG CTCCAGATCG CCATGTTTCA GTGGCAGCAC 
GAAGATCTCC TAATCACCCG ACCACAAGCC ATGTCTGTGA CTCCTCCACC TCGCTCTGCT 
ATTCTAACAA ATATGAAAAA GAAGCTGCAT ACCCTGAGCC AGATCGAAAC GTCCATTGCA 
GCGGTTCAGG AGAAACTAGC AGCCCTCGAA GCAAGCATCG AACAACGTTT GAAGTGGGCA 
GGAGGGGCCA ATCCCGCATT GGCTCCAGTC CTACAAGATT TTGAGGCAAC AATAGCGGAA 
AGAAGGAATC TCGTTCTGAA AGAAAGCCAA CGAGCAAATC AGGTCACTTT CCTCTGCAGC 
AATATCATTC ATTTTGAAAG CCTACGAACG CGAACTGCCG AAGCTCTAAA TCTCGATGCT 
GCCTTGTTTG AACTCATCAA GCGCTGTCAA CAGATGTGTT CGTTTGCCTC ACAGTTTAAC 
AGCTCGGTTT CTGAACTGGA ACTGCGTTTA CTGCAAAGAG TGGGTTCCGG TCTCGAACCT 
CCCATTGGCA GCCCTGAATG GCTTGTTTCT GCACACAAAC AACTGACCCA GGATATGTCT 
ACTCAGAGGG CGGTTCAAAC AGAAAAAGAA CAGCAAATCG AATCAGTCTG TGAAGCAATT 
CAGAACCTTG TGGATAGTAT TAAGACAGTG CTTACCGGTC ACAACAGGCA ACTTGGAGAT 
GTTAAACATC TATTGAAAGC AATGGCCAAG GATGAAGAGG CTGCTTTGGC AGATGGTGAA 
GATGTTCCCT ATGAGAACAG TGTTAGACAA TTCTTGGGTG AATATAAGTC ATGGCAAGAC 
AATATTCAAA CGGTATTGTT TACATTAGTT CAGGCTATGG GGCAGGTTCG CAGCCAAGAG 
CATGTGGAGA TGCTGCAGGA GATAACTCCC ACCTTGAAAG AGCTGAAAAC ACAAAGCCAG 
AGTATCTACA ATAATCTAGT GAGTTTTGCA TCACCCTTAG TCACAGATGC AACAAATGAG 
TGTTCCAGCC CAACATCATC TGCTACGTAT CAGCCAACCT TTGCTGCAGC GGTCCGCAGT 
AACACTGGCC AGAAGACTCA GCCTGAAGTT ATGTCGCAGA ATGCAAGGAA GCTGATTCAG 
AAAAATCTTG CAACATCAGC AGATACCCCA CCAAGCACAG TTCCAGGAAC TGGCAAGAGT 
GTGGCCTGTA GTCCTAAAAA AGCAGTCAGG GATCCAAAAA CTGGAAAAGC TGTGCAAGAA 
AGGAACTCCT ATGCAGTGAG TGTGTGGAAG CGAGTCAAAG CCAAGTTAGA GGGCCGAGAT 
GTGGATCCGA ACAGGAGGAT GTCTGTTGCT GAACAGGTGG ACTATGTGAT TAAGGAAGCA 
ACTAATCTAG ATAACTTGGC TCAGCTGTAT GAAGGTTGGA CGGCCTGGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028913886.1
CDS	201..11174
Translation	MSRRAPGSRLSGGGTKYPRSWNDWPPRTDSAPADQDHLKYSSSRDRGSSASYGLQPSNSVVVSRQRHDDIRVHTDIQNEEKGGYNVNGGAGENTYGRKSLGQELRVNNVTNPDFTSVQHGNRALATKDMRKSQERSLSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKQVLVKQLDNILTAIHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSTKDEVKLLYLCATFKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGHPITEAYVTDVLYRVMRCVTTANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPASKLLLLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHNLQLPEACSEIQHDAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLLEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNSNHTEIQEISLAIRSHMSKTPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNSISQGNELEVIIMMVVEALCELHCPEAIQGLAVWSSAVVGKSLVWINSVAQQAEGRFEKATVEYQEHLCAMTGVDCCITGFDKSILKLANMNTNNTSPKHSLNGDARKTVLAKPSDSSPEVINYLGNKACECYISLADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFLECTEQLELLPGENINLLASGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATAVSHIEQEQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLYSEALRSCKQHDVRPWMQALRYTVYQSQLVEKIKEQTVPIRSHLMELGLTAAKFARKRGNVALATRLLAQCSEVRLGKTTTAQDLVQYFKKLSTQGQIDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNTTGLSTLSKNIHTLIELPSVNTTEEEYPRIESESTVNIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDNITEEDREKIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLLTSCPWLSDLDENATEGLIKVWRKIVDRIFSLYKLSCSAYFTFLKLNAGQVTLDEDDPRLHLSIRSEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQTSGNKLPSAIPTLLGNIQGEELLGAECEGGSPPASQDSNKDEPKNGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGEAIENALEKLKNPLNPAKPGSSWIPFKEVMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKNYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLNVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGVFRLSCEQVLQIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLIVLPKLDGSLEEYLSLQEQLTDVEKLQGKLVEEIDFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRTVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATTFLQNAGQAHLISQCEQLEGEVGAFLQQRRSVLRGCLEQLHNYATVALQYPKTIFQKHRIEQWKTWMEELIRNTTIERCQELYRKYELQYAPQPPPTVCQFITATEMTLQRYAADINNRLIRQVERLKQEAVTVLVCEEQLKEIERCIKVFLHENGEEGSWSLASVIISALCALTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPLEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDNLIEQTTDGVPLQTLVDSLQAYLRNAAMGLEEETHSHYIDVARILHSQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLIEKLNKMEVPVSWRKIDIIREARSTQINFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQSPVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSFKTSLQRVQLQIAMFQWQHEDLLITRPQAMSVTPPPRSAILTNMKKKLHTLSQIETSIAAVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRANQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVGSGLEPPIGSPEWLVSAHKQLTQDMSTQRAVQTEKEQQIESVCEAIQNLVDSIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPTFAAAVRSNTGQKTQPEVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_029058053.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029058053.1

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCGGCGGCG GCACCAAGTA CCCGCGGAGC 
TGGAATGACT GGCCACCCAG AACTGATAGT GCACCAGCCG ACCAGGATCA TTTAAAATAT 
TCTTCGTCTA GAGATCGGGG CAGTTCTGCC TCTTATGGAT TACAACCTTC AAATTCAGTT 
GTGGTGTCTC GTCAACGGCA CGATGATATC AGAGTCCACA CCGACATACA GAATGAAGAA 
AAGGGTGGCT ACAATGTCAA TGGAGGAGCT GGGGAAAATA CTTATGGTCG GAAGTCGTTG 
GGGCAAGAGC TGAGGGTTAA CAATGTGACC AACCCTGATT TCACCAGTGT TCAGCATGGA 
AATCGTGCTT TAGCCACAAA AGACATGAGG AAATCACAGG AGCGATCGTT GTCTTACTCT 
GACGAGTCTC GACTGTCAAA CCTTCTTCGG AGGATTACCC GGGAAGACGA CAGAGACCGA 
AGACTGGCTA CTGTGAAGCA ATTGAAAGAA TTCATTCAGC AACCAGAAAA TAAGCAGGTC 
TTAGTTAAAC AACTGGATAA TATCCTGACC GCTATACATG ATGTGCTTAA TGAAAGTAGC 
AAATTGCTTC AGGAATTGAG GCAGGAGGGA GCTTGCTGTC TTGGCCTTCT TTGTGCTTCT 
TTGAGTTATG AGGCCGAGAA GATCTTCAAA TGGATTTTTA GCAAATTTAG CTCGTCCACA 
AAAGATGAAG TTAAACTGCT TTACTTATGT GCCACCTTCA AAGCACTAGA GACTGTAGGA 
GAAAAGAAAG CCTTTTCATC TGTAATGCAG CTTGTAATGA CCAGCCTGCA GTCCATTCTA 
GAAAATGTGG ATACACCAGA ATTGCTATGC AAATGTGTGA AGTGCATTCT TCTGGTGGCC 
CGATGTTACC CTCATATTTT CAGCACTAAT TTTAGGGATA CGGTTGACAT ATTGGTTGGG 
TGGCATATAG ATCACACTCA GAAACCTTCC TTGACACAGC AAGTGTCTGG ATGGCTGCAG 
AGTCTGGAGC CCTTTTGGGT AGCCGATCTT GCATTTTCCA CCACCCTTCT CGGTCAGTTT 
CTGGAAGATA TGGAGGCTTA TGCTGAAGAT CTCAGTCATG TGGCATCTGG GGAATCGGTC 
GATGAAGACA TTCCTCCTCC ATCAGTGTCC TTACCGAAGT TGGCGGCTCT TCTCCGGGTT 
TTCAGTACTG TGGTGCGGAG TATTGGGGAG CGCTTCAGTC CAATTAGAGG ACATCCAATT 
ACCGAGGCTT ATGTAACAGA TGTTTTGTAC AGAGTAATGA GATGTGTGAC AACAGCAAAC 
CAAGTATTTT TTTCTGAAGC TGTATTGACA GCTGCCAATG AATGTGTCGG TGTATTGCTT 
GGTAGCCTAG ATCCTAGTAT GACTATTCAT TGTGACATGG TCATCACCTA TGGACTAGAC 
CAACTGGAGA ATTGCCAGAC TTGTGGTACA GATTATATCA TCTCGGTCTT AAATTTACTG 
ACGTTGATTG TTGAGCAGAT CAATACAAAG CTGCCATCGT CATTCGTCGA AAAACTGTTT 
ATACCAGCGT CTAAACTACT GCTTCTGCGC TATCATAAAG AAAAAGAGGT TGTTGCAGTA 
GCTCATGCTG TATATCAAGC CGTTCTTAGC CTGAAGAACA TTCCCGTCTT GGAGACCGCC 
TATAAGTTAA TTTTAGGAGA AATGACTTGT GCACTGAATA ATCTCCTGCA TAACCTTCAA 
CTTCCTGAGG CCTGTTCCGA AATCCAACAC GATGCTTTTA AGAATCACGT TTTCAATGTG 
GACAATGCCA AATTTGTAGT TATATTTGAC CTCAGTGCCC TGACCACGAT TGGAAATGCG 
AAAAACTCAT TAATTGGGAT GTGGGCACTC TCGCCAACCG TTTTTGCACT TCTGAGCAAG 
AATCTGATGA TAGTGCATGG AGATCTCGCC GTTCACTTCC CAGCTATTCA GTATGCCGTT 
CTCTACACTT TGTATTCACA TTGTACCAGA CATGATCACT TCATTTCTAG CAGTCTTAGC 
TCCTCTTCTC CTTCTCTCTT TGATGGAGCA GTTATCAGCA CTGTCACCAC AGCTACAAAG 
AAGCATTTCT CAATCATATT AAACCTCCTG GGAATATTAC TGAAGAAAGA TAATCTTAAT 
CAGGATACCA GGAAACTACT AATGACCTGG GCTTTGGAAG TTGCTGTTCT AATGAAAAAG 
TCTGAAACAT ATGCACCTTT GTTCTCTCTT CCGTCGTTCC ATAAATTTTG CAAAGGACTT 
CTAGCAAACA CTCTTCTTGA AGATGTGAAT ATCTGCCTTC AAGCATGTAG TAGTCTGCAT 
GCCTTGTCAT CTTCCCTGCC AGATGACCTT TTACAAAGGT GTGTTGATGT GTGCCGCGTT 
CAGCTGGTCC ATAGTGGAAC TCGGATTCGA CAAGCCTTTG GAAAGCTGTT GAAGTCTATT 
CCGTTAGATG TTGTCTTGAG CAATAGCAAC CACACAGAGA TTCAAGAAAT TTCCTTAGCT 
ATAAGGAGTC ACATGAGCAA AACTCCAAGT AATACTTTCC ATCCACAAGA TTTCTCTGAC 
GTGATAAGTT TTATTTTGTA TGGAAACTCT CATAGAACCG GGAAGGACAA TTGGTTAGAA 
AGATTGTTCT ACAGCTGTCA GAGATTGGAT AAGCGTGATC AGTCAACCAT TCCACGCAAT 
CTACTCAAAA CAGATGCCGT CCTCTGGCAG TGGGCTATTT GGGAAGCTGC TCAGTTCACT 
GTTCTCTCTA AACTAAGAAC CCCGCTGGGC AGAGCTCAAG ATACCTTCCA GACAATCGAA 
GGTATCATTA GAAGTCTTGC CGCTCATACA TTAAACCCCG ATCAAGATGT TAGCCAGTGG 
ACCACTGCGG ACAATGATGA AGGACATAGC AGCAACCAAC TCAGACTTGT TCTTCTACTG 
CAGTACTTGG AAAATTTGGA AAAACTAATG TATAATGCAT ATGAAGGTTG TGCTAATGCA 
CTGACTTCCC CTCCTAAGGT TATCAGGACA TTTTTCTATA CCAATCGCCA GACATGTCAG 
GACTGGTTAA CTCGGATTAG ACTTTCCATC ATGAGAGTTG GATTGTTGGC CGGTCAGCCC 
GCTGTGACAG TAAGACACGG TTTTGATTTG CTCACCGAAA TGAAGACCAA TAGTATCTCT 
CAGGGAAATG AGCTAGAAGT GATTATTATG ATGGTCGTAG AAGCACTTTG TGAGCTTCAC 
TGTCCTGAAG CTATACAGGG ATTAGCTGTC TGGTCTTCAG CAGTGGTTGG AAAGAGTCTC 
GTCTGGATTA ATTCAGTGGC ACAGCAAGCC GAAGGGCGGT TTGAAAAGGC AACTGTTGAA 
TACCAGGAGC ACTTGTGTGC TATGACAGGG GTAGATTGCT GCATAACTGG GTTTGATAAA 
TCCATTCTGA AGTTGGCTAA CATGAATACT AACAATACCA GCCCCAAACA TTCTCTCAAT 
GGTGACGCTA GGAAAACTGT TCTGGCCAAA CCAAGTGATT CTTCTCCTGA GGTTATTAAT 
TACTTGGGTA ACAAAGCATG TGAGTGCTAC ATCTCTCTTG CTGATTGGGC TGCTGTGCAG 
GAATGGCAGA ATGCTGTCCA TGACTTGAAA AAGAGCACAA GTAGTACTTC CCTCAATTTA 
AAAGCTGACT TTAACTACAT AAAATCACTG AGCAGCTTTG AATCTGGCGA ATTTCTCGAA 
TGTACAGAAC AACTAGAATT GCTGCCAGGA GAAAACATTA ACTTACTTGC CAGCGGGTCA 
AAGGAAAAAA TAGATATGAA AAAGCTACTT CCAAACATGT TAAGCCCAGA TCCACGGGAA 
CTTCAGAAAT CCATTGAAGT TCAGTTGCTG AGAAGTTCCG TTTGTTTGGC TACCGCTGTA 
AGCCACATAG AGCAGGAACA AAAGTGGCAA TCTATAACTG AAAACGTTGT AAAATATTTG 
AAGCAAACAT CAAGAATAGC TATTGGACCA CTGAGACTTT CCACTTTAAC TGTTTCACAG 
TCCTTACCAG TTCTAAGTAC CCTACAGCTG TATTGTTCTT CTGCTTTGGA GAACACCGTG 
TCAAACAGGC TTTCGACAGA GGACTGCTTA ATCCCACTCT ATAGTGAAGC TTTACGTTCG 
TGTAAACAGC ATGATGTAAG GCCCTGGATG CAGGCTTTGA GGTATACAGT ATACCAGAGT 
CAGTTGGTGG AAAAAATCAA AGAACAAACG GTTCCAATTA GAAGTCACCT CATGGAATTG 
GGTTTAACAG CTGCGAAATT TGCTCGTAAA CGTGGAAACG TTGCCCTGGC AACCAGATTG 
CTCGCTCAAT GTAGTGAAGT GCGGTTGGGA AAGACGACCA CTGCTCAAGA TTTAGTCCAG 
TATTTTAAAA AACTGTCAAC ACAAGGTCAA ATAGATGAAA AATGGGGACC TGAACTGGAT 
ATTGAGAAAA CGAAATTGTT ATACACAGCA GGTCAGTCAA CCCATGCGAT GGAAATGCTG 
AGTTCTTGCG CCATATCCTT CTGCAAGTCT GCCAAAGCTG AATACGCAGT AGCCAAATCG 
ATTCTTACAC TTGCTAAATG GATCCAAGCT GAATGGAAAG AAATTTCAGG ACAGTTGAAA 
CAGGTGTACA GAGCCCAACA GCAGCAGAAC ACCACGGGCC TGTCTACATT ATCTAAAAAC 
ATACACACTT TGATCGAACT GCCGTCTGTC AACACCACAG AAGAGGAGTA TCCCCGCATC 
GAGAGTGAAT CAACAGTCAA CATTGGAGTT GGGGAACCGG ACTTCATCCT GGGCCAGTTG 
TATCACCTGT CTTCAGTGCA GGCACCCGAA GTGGCCAAAT CTTGGGCAGC GCTGGCAAGT 
TGGGCTTACA GATGGGGAAG AAAGGTGGTG GATAACGCCA GTCAGGGAGA AGGTGTTCGA 
CTACTGCCAA GAGAGAAATC TGAGGTTCAA AATCTGCTTC CAGACAACAT AACAGAGGAG 
GATCGAGAGA AGATCTACGG CATCCTTGGG CAGGCGGTGT GTCGGCCAGC CGGAATTCAG 
GATGAAGATA TAACACTGCA GATAACAGAG AGTGAAGACA ATGAGGAAGA TGACATGGTG 
GATGTGATCT GGCGTCAGTT ATTGACGAGT TGCCCGTGGC TTTCCGATCT TGATGAAAAT 
GCCACAGAAG GCCTAATTAA AGTGTGGAGA AAAATTGTAG ATAGGATCTT CAGTCTCTAC 
AAACTGTCCT GCAGTGCCTA TTTTACTTTC CTTAAACTCA ATGCTGGCCA GGTTACTCTA 
GATGAAGATG ACCCAAGGCT GCACTTGAGC ATTAGGTCTG AACAAAGCAC TGACGATGTT 
ATTGTAATGG CAACGTTAAG ACTGTTGAGA TTGCTGGTCA AACACGCTGG AGAGCTAAGG 
CAGTATCTGG AGCACGGCCT AGAAACCACC CCCACTGCTC CTTGGAGAGG TATCATTCCA 
CAGCTCTTCT CCCGTCTAAA TCATCCCGAA GTGTACGTCC GCCAAAGTAT TTGTAATCTA 
CTCTGTCGAG TGGCACAAGA TTCTCCTCAT CTCATATTAT ATCCTGCAAT TGTGGGAACT 
ATTTCACTGA GTAGTGAATC GCAGACATCA GGCAATAAGT TGCCTTCAGC CATCCCCACT 
TTGCTGGGCA ATATTCAAGG CGAAGAATTA CTCGGAGCGG AATGTGAAGG GGGAAGTCCT 
CCAGCTTCTC AAGACAGCAA TAAAGATGAA CCCAAGAATG GCTTAAATGA AGATCAAGCG 
ATGATGCAGG ATTGCTACAG CAAAATTGTT GATAAATTGT CTTCTGCCAA CCCAACAATG 
GTCTTACAGG TTCAAATGCT GGTGGCCGAG TTGCGCAGGG TGACCGTTCT TTGGGATGAG 
CTCTGGCTGG GAGTTCTTCT ACAACAACAT ATGTATGTTC TGAGGCGCAT TCAACAGTTG 
GAGGACGAAG TAAAGAGAGT CCAGAATAAC AATACGTTAC GCAAAGAAGA GAAGATTGCA 
ATAATGCGAG AAAAGCACAC AGCTCTGATG AAGCCCATCG TATTTGCTTT GGAACATGTA 
CGGAGCATCA CTGCCGCTCC TGCCGAAACA CCTCATGAGA AGTGGTTCCA AGACAACTAT 
GGTGAGGCAA TTGAAAATGC CCTGGAAAAA CTCAAGAACC CATTAAATCC TGCAAAACCC 
GGAAGCAGTT GGATCCCATT TAAAGAGGTG ATGCTAAGTT TGCAGCAGAG AGCACAGAAA 
CGTGCTAGTT ATATCTTGCG CCTTGAAGAA ATCAGCCCCT GGTTGGCTGC CATGACTAAT 
ACTGAAATTG CCCTTCCTGG AGAAGTATCA GCCAGAGATA CCGTGACGAT CCACAGCGTG 
GGCGGAACCA TCACAATCCT ACCTACTAAA ACCAAACCCA AAAAACTGCT CTTTCTGGGT 
TCAGACGGGA AAAACTACCC CTATCTCTTT AAAGGACTGG AAGATCTGCA CCTGGATGAG 
AGAATCATGC AGTTTCTATC TATCGTTAAT ACCATGTTCG CTACCATCAA TCGCCAAGAG 
ACGCCCCGTT TCCATGCCAG GCACTATTCG GTGACCCCGC TGGGGACGAG GTCGGGCCTG 
ATTCAGTGGG TAGATGGAGC CACTCCCTTA TTTGGCCTTT ACAAGCGATG GCAACAGCGA 
GAAGCTGCTT TACAAGCGCA AAAGGCCCAG GATTCTTACC AGACTCCTCA AAATCCTGGA 
ATAGTACCTC GGCCCAGCGA GCTTTATTAC AGTAAAATCG GGCCCGCCCT GAAAGCGGTC 
GGACTCAGTC TCGACGTGTC CCGGCGAGAC TGGCCTCTCA ACGTGATGAA GGCTGTGTTG 
GAAGAACTGA TGGAAGCCAC CCCTCCAAAC CTTCTGGCCA AGGAGCTTTG GTCTTCTTGT 
ACTACACCTG ACGAATGGTG GAGAGTTACC CAGTCTTACG CAAGATCCAC CGCGGTCATG 
TCAATGGTTG GATACATAAT CGGCCTTGGA GATAGACATC TGGATAACGT TCTTATAGAC 
ATGACTACAG GAGAAGTTGT TCACATAGAT TACAATGTTT GTTTTGAAAA AGGTAAAAGT 
CTGAGAGTGC CGGAGAAAGT TCCTTTCAGG ATGACTCACA ACATCGAAAC AGCTCTCGGT 
GTAACTGGAG TTGAGGGAGT TTTCAGGCTT TCTTGTGAAC AGGTTCTCCA AATTATGCGG 
CGTGGAAGAG AGACCCTGCT CACGTTGCTG GAAGCCTTTG TGTACGATCC TCTTGTGGAC 
TGGACAGCCG GAGGGGAAGC AGGATTTGCA GGAGCTGTCT ATGGCGGAGG CGGCCAGCAG 
GCAGAGAACA AGCAAAGTAA AAGGGAGATG GAAAGGGACA TCACCCGCAG CCTCTTTTCT 
TCCCGAGTAG CTGAAATCAA GGTGAACTGG TTTAAAAACA GAGATGAGAT GTTGATTGTG 
CTTCCAAAGC TAGATGGAAG CTTAGAAGAA TACCTGAGCC TACAAGAGCA ATTGACAGAT 
GTTGAAAAAC TGCAAGGCAA ACTCGTTGAG GAGATTGACT TTCTAGAAGG AGCCGAAGGA 
GTAGATCATC CCTCGCACAC TCTGCAGCAC AGGTATTCCG AACACACCCA ACTGCAGACT 
CAGCAGAGAA CCGTTCAAGA AGCCATCCAG GTGAAGTTGA ATGAATTTGA GCAGTGGATA 
ACACATTACC AAGCTGCGTT TAACAATTTA GAAGCCACCC AGCTGGCCAG TCTGCTTCAG 
GAGATTAGCA CCCAAATGGA CCTGGGTCCT CCAAGCTACG TGCCTGCAAC CACCTTCCTG 
CAGAACGCTG GCCAAGCCCA CTTGATCAGT CAGTGCGAGC AGCTGGAGGG CGAGGTTGGA 
GCTTTCCTTC AGCAGAGGCG CTCCGTCCTG CGAGGCTGTC TCGAGCAGCT GCATAACTAC 
GCGACCGTAG CCCTTCAGTA CCCGAAAACC ATATTCCAGA AACACCGAAT CGAGCAGTGG 
AAGACCTGGA TGGAAGAACT TATACGCAAT ACGACCATCG AGAGATGTCA GGAGTTGTAC 
CGGAAATATG AACTCCAGTA TGCCCCCCAG CCACCGCCCA CGGTGTGTCA GTTCATAACT 
GCCACTGAAA TGACTCTTCA GCGCTACGCG GCAGACATAA ATAATAGGCT TATCAGACAA 
GTGGAGCGCC TGAAACAAGA AGCAGTGACC GTGCTGGTCT GCGAGGAGCA GTTAAAAGAA 
ATTGAACGGT GTATTAAGGT TTTTCTTCAT GAAAACGGAG AGGAGGGGTC CTGGAGCCTG 
GCGAGCGTTA TCATCTCTGC ACTCTGTGCA CTTACAAGGC GCAACTTAAT GATGGAAGGT 
GCCGCTTCTA GTGCTGGCGA GCAGCTGGTC GATCTGACTT CCCGAGATGG GGCCTGGTTC 
TTGGAGGAGC TGTGCAGCAT GAGTGGAAAT GTAACCTGCC TTGTACAGTT ACTGAAGCAG 
TGTCACCTGG TCCCTCAGGA TTTAGATATT CCCAATCCCC TCGAAGCTTC GGAGGCAGTT 
CACTTAGCTA ATGGAGTCTA CACCTCGCTT CAGGAATTGA ACTCCAACTT CCGGCAGATC 
ATCTTTCCGG AAGCTCTGCG ATGTTTAATG AAAGGGGAAT ACACTTTAGA AAGCATGCTG 
CACGAACTGG ACAACCTCAT TGAACAGACA ACTGATGGAG TTCCGTTGCA AACCCTGGTT 
GACTCTCTTC AGGCCTATTT AAGAAATGCA GCTATGGGCC TGGAAGAAGA GACACATTCT 
CACTATATTG ATGTTGCCAG AATACTCCAT TCTCAGTATG GCGAATTGAT TCAACCAAGG 
AATGGTTCAG TTGATGAAAC ACCAAAAATG TCCGCCGGTC AAATGCTTTT GGTTGCGTTC 
GATGGAATGT TCGCCCAAGT GGAAACCGCT TTTGGCTTGT TAATTGAAAA GTTAAACAAG 
ATGGAAGTCC CCGTATCGTG GCGAAAGATA GACATCATAA GAGAAGCCAG AAGCACCCAG 
ATTAATTTCT TTGACGACGA TAATCACCGA CAGGTTCTGG AGGAGATTTT CTTTCTCAAA 
AGACTGCAGA CCATCAAAGA ATTCTTTAGA CTCTGTGGTA CTTTTTCTAA AACGCTGTCA 
GGATCGAGTT CGCTCGAAGA TCAGAGCCCG GTGAATGGAC CCGTTCAAAT TGTTAATGTG 
AAAACGCTTT TCAGAAACTC TTGTTTTAGT GAAGATCAGA TGGCCAAACC CATAAAGGCG 
TTCACAGCTG ACTTCGTGAG GCAGCTCTTA ATTGGCCTGC CTAATCAAGC CTTGGGGCTA 
ACCCTTTGCA GTTTCATCAG CGCGCTGGGC GTCGACATCA TCGCCCAGGT CGAGGCAAAG 
GACTTTGGGG CAGAGAGCAA GGTTTCGGTG GACGATCTGT GTAAGAAAGC CGTGGAGCAC 
AACATCCAGA TTGGAAAGTT CTCCCAGCTG GTCATGAACC GAGCCACGGT CCTGGCCAGT 
TCCTACGACA CTGCTTGGAA GAAGCACGAT TTGGTGCGCA GGCTAGAAAC CAGTATTTCC 
TCTTTCAAGA CCAGCCTGCA GAGAGTGCAG CTCCAGATCG CCATGTTTCA GTGGCAGCAC 
GAAGATCTCC TAATCACCCG ACCACAAGCC ATGTCTGTGA CTCCTCCACC TCGCTCTGCT 
ATTCTAACAA ATATGAAAAA GAAGCTGCAT ACCCTGAGCC AGATCGAAAC GTCCATTGCA 
GCGGTTCAGG AGAAACTAGC AGCCCTCGAA GCAAGCATCG AACAACGTTT GAAGTGGGCA 
GGAGGGGCCA ATCCCGCATT GGCTCCAGTC CTACAAGATT TTGAGGCAAC AATAGCGGAA 
AGAAGGAATC TCGTTCTGAA AGAAAGCCAA CGAGCAAATC AGGTCACTTT CCTCTGCAGC 
AATATCATTC ATTTTGAAAG CCTACGAACG CGAACTGCCG AAGCTCTAAA TCTCGATGCT 
GCCTTGTTTG AACTCATCAA GCGCTGTCAA CAGATGTGTT CGTTTGCCTC ACAGTTTAAC 
AGCTCGGTTT CTGAACTGGA ACTGCGTTTA CTGCAAAGAG TGGGTTCCGG TCTCGAACCT 
CCCATTGGCA GCCCTGAATG GCTTGTTTCT GCACACAAAC AACTGACCCA GGATATGTCT 
ACTCAGAGGG CGGTTCAAAC AGAAAAAGAA CAGCAAATCG AATCAGTCTG TGAAGCAATT 
CAGAACCTTG TGGATAGTAT TAAGACAGTG CTTACCGGTC ACAACAGGCA ACTTGGAGAT 
GTTAAACATC TATTGAAAGC AATGGCCAAG GATGAAGAGG CTGCTTTGGC AGATGGTGAA 
GATGTTCCCT ATGAGAACAG TGTTAGACAA TTCTTGGGTG AATATAAGTC ATGGCAAGAC 
AATATTCAAA CGGTATTGTT TACATTAGTT CAGGCTATGG GGCAGGTTCG CAGCCAAGAG 
CATGTGGAGA TGCTGCAGGA GATAACTCCC ACCTTGAAAG AGCTGAAAAC ACAAAGCCAG 
AGTATCTACA ATAATCTAGT GAGTTTTGCA TCACCCTTAG TCACAGATGC AACAAATGAG 
TGTTCCAGCC CAACATCATC TGCTACGTAT CAGCCAACCT TTGCTGCAGC GGTCCGCAGT 
AACACTGGCC AGAAGACTCA GCCTGAAGTT ATGTCGCAGA ATGCAAGGAA GCTGATTCAG 
AAAAATCTTG CAACATCAGC AGATACCCCA CCAAGCACAG TTCCAGGAAC TGGCAAGAGT 
GTGGCCTGTA GTCCTAAAAA AGCAGTCAGG GATCCAAAAA CTGGAAAAGC TGTGCAAGAA 
AGGAACTCCT ATGCAGTGAG TGTGTGGAAG CGAGTCAAAG CCAAGTTAGA GGGCCGAGAT 
GTGGATCCGA ACAGGAGGAT GTCTGTTGCT GAACAGGTGG ACTATGTGAT TAAGGAAGCA 
ACTAATCTAG ATAACTTGGC TCAGCTGTAT GAAGGTTGGA CGGCCTGGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_029058054.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028913887.1 Ornithorhynchus anatinus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

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CloneID	OOc122578
Clone ID Related Accession (Same CDS sequence)	XM_029058054.1
Accession Version	XM_029058054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCACCA GCCGACCAGG ATCATTTAAA ATATTCTTCG 
TCTAGAGATC GGGGCAGTTC TGCCTCTTAT GGATTACAAC CTTCAAATTC AGTTGTGGTG 
TCTCGTCAAC GGCACGATGA TATCAGAGTC CACACCGACA TACAGAATGA AGAAAAGGGT 
GGCTACAATG TCAATGGAGG AGCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAACCCT GATTTCACCA GTGTTCAGCA TGGAAATCGT 
GCTTTAGCCA CAAAAGACAT GAGGAAATCA CAGGAGCGAT CGTTGTCTTA CTCTGACGAG 
TCTCGACTGT CAAACCTTCT TCGGAGGATT ACCCGGGAAG ACGACAGAGA CCGAAGACTG 
GCTACTGTGA AGCAATTGAA AGAATTCATT CAGCAACCAG AAAATAAGCA GGTCTTAGTT 
AAACAACTGG ATAATATCCT GACCGCTATA CATGATGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAAT TGAGGCAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTTTGAGT 
TATGAGGCCG AGAAGATCTT CAAATGGATT TTTAGCAAAT TTAGCTCGTC CACAAAAGAT 
GAAGTTAAAC TGCTTTACTT ATGTGCCACC TTCAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCCAT TCTAGAAAAT 
GTGGATACAC CAGAATTGCT ATGCAAATGT GTGAAGTGCA TTCTTCTGGT GGCCCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACGGTTG ACATATTGGT TGGGTGGCAT 
ATAGATCACA CTCAGAAACC TTCCTTGACA CAGCAAGTGT CTGGATGGCT GCAGAGTCTG 
GAGCCCTTTT GGGTAGCCGA TCTTGCATTT TCCACCACCC TTCTCGGTCA GTTTCTGGAA 
GATATGGAGG CTTATGCTGA AGATCTCAGT CATGTGGCAT CTGGGGAATC GGTCGATGAA 
GACATTCCTC CTCCATCAGT GTCCTTACCG AAGTTGGCGG CTCTTCTCCG GGTTTTCAGT 
ACTGTGGTGC GGAGTATTGG GGAGCGCTTC AGTCCAATTA GAGGACATCC AATTACCGAG 
GCTTATGTAA CAGATGTTTT GTACAGAGTA ATGAGATGTG TGACAACAGC AAACCAAGTA 
TTTTTTTCTG AAGCTGTATT GACAGCTGCC AATGAATGTG TCGGTGTATT GCTTGGTAGC 
CTAGATCCTA GTATGACTAT TCATTGTGAC ATGGTCATCA CCTATGGACT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACAGATTAT ATCATCTCGG TCTTAAATTT ACTGACGTTG 
ATTGTTGAGC AGATCAATAC AAAGCTGCCA TCGTCATTCG TCGAAAAACT GTTTATACCA 
GCGTCTAAAC TACTGCTTCT GCGCTATCAT AAAGAAAAAG AGGTTGTTGC AGTAGCTCAT 
GCTGTATATC AAGCCGTTCT TAGCCTGAAG AACATTCCCG TCTTGGAGAC CGCCTATAAG 
TTAATTTTAG GAGAAATGAC TTGTGCACTG AATAATCTCC TGCATAACCT TCAACTTCCT 
GAGGCCTGTT CCGAAATCCA ACACGATGCT TTTAAGAATC ACGTTTTCAA TGTGGACAAT 
GCCAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACCA CGATTGGAAA TGCGAAAAAC 
TCATTAATTG GGATGTGGGC ACTCTCGCCA ACCGTTTTTG CACTTCTGAG CAAGAATCTG 
ATGATAGTGC ATGGAGATCT CGCCGTTCAC TTCCCAGCTA TTCAGTATGC CGTTCTCTAC 
ACTTTGTATT CACATTGTAC CAGACATGAT CACTTCATTT CTAGCAGTCT TAGCTCCTCT 
TCTCCTTCTC TCTTTGATGG AGCAGTTATC AGCACTGTCA CCACAGCTAC AAAGAAGCAT 
TTCTCAATCA TATTAAACCT CCTGGGAATA TTACTGAAGA AAGATAATCT TAATCAGGAT 
ACCAGGAAAC TACTAATGAC CTGGGCTTTG GAAGTTGCTG TTCTAATGAA AAAGTCTGAA 
ACATATGCAC CTTTGTTCTC TCTTCCGTCG TTCCATAAAT TTTGCAAAGG ACTTCTAGCA 
AACACTCTTC TTGAAGATGT GAATATCTGC CTTCAAGCAT GTAGTAGTCT GCATGCCTTG 
TCATCTTCCC TGCCAGATGA CCTTTTACAA AGGTGTGTTG ATGTGTGCCG CGTTCAGCTG 
GTCCATAGTG GAACTCGGAT TCGACAAGCC TTTGGAAAGC TGTTGAAGTC TATTCCGTTA 
GATGTTGTCT TGAGCAATAG CAACCACACA GAGATTCAAG AAATTTCCTT AGCTATAAGG 
AGTCACATGA GCAAAACTCC AAGTAATACT TTCCATCCAC AAGATTTCTC TGACGTGATA 
AGTTTTATTT TGTATGGAAA CTCTCATAGA ACCGGGAAGG ACAATTGGTT AGAAAGATTG 
TTCTACAGCT GTCAGAGATT GGATAAGCGT GATCAGTCAA CCATTCCACG CAATCTACTC 
AAAACAGATG CCGTCCTCTG GCAGTGGGCT ATTTGGGAAG CTGCTCAGTT CACTGTTCTC 
TCTAAACTAA GAACCCCGCT GGGCAGAGCT CAAGATACCT TCCAGACAAT CGAAGGTATC 
ATTAGAAGTC TTGCCGCTCA TACATTAAAC CCCGATCAAG ATGTTAGCCA GTGGACCACT 
GCGGACAATG ATGAAGGACA TAGCAGCAAC CAACTCAGAC TTGTTCTTCT ACTGCAGTAC 
TTGGAAAATT TGGAAAAACT AATGTATAAT GCATATGAAG GTTGTGCTAA TGCACTGACT 
TCCCCTCCTA AGGTTATCAG GACATTTTTC TATACCAATC GCCAGACATG TCAGGACTGG 
TTAACTCGGA TTAGACTTTC CATCATGAGA GTTGGATTGT TGGCCGGTCA GCCCGCTGTG 
ACAGTAAGAC ACGGTTTTGA TTTGCTCACC GAAATGAAGA CCAATAGTAT CTCTCAGGGA 
AATGAGCTAG AAGTGATTAT TATGATGGTC GTAGAAGCAC TTTGTGAGCT TCACTGTCCT 
GAAGCTATAC AGGGATTAGC TGTCTGGTCT TCAGCAGTGG TTGGAAAGAG TCTCGTCTGG 
ATTAATTCAG TGGCACAGCA AGCCGAAGGG CGGTTTGAAA AGGCAACTGT TGAATACCAG 
GAGCACTTGT GTGCTATGAC AGGGGTAGAT TGCTGCATAA CTGGGTTTGA TAAATCCATT 
CTGAAGTTGG CTAACATGAA TACTAACAAT ACCAGCCCCA AACATTCTCT CAATGGTGAC 
GCTAGGAAAA CTGTTCTGGC CAAACCAAGT GATTCTTCTC CTGAGGTTAT TAATTACTTG 
GGTAACAAAG CATGTGAGTG CTACATCTCT CTTGCTGATT GGGCTGCTGT GCAGGAATGG 
CAGAATGCTG TCCATGACTT GAAAAAGAGC ACAAGTAGTA CTTCCCTCAA TTTAAAAGCT 
GACTTTAACT ACATAAAATC ACTGAGCAGC TTTGAATCTG GCGAATTTCT CGAATGTACA 
GAACAACTAG AATTGCTGCC AGGAGAAAAC ATTAACTTAC TTGCCAGCGG GTCAAAGGAA 
AAAATAGATA TGAAAAAGCT ACTTCCAAAC ATGTTAAGCC CAGATCCACG GGAACTTCAG 
AAATCCATTG AAGTTCAGTT GCTGAGAAGT TCCGTTTGTT TGGCTACCGC TGTAAGCCAC 
ATAGAGCAGG AACAAAAGTG GCAATCTATA ACTGAAAACG TTGTAAAATA TTTGAAGCAA 
ACATCAAGAA TAGCTATTGG ACCACTGAGA CTTTCCACTT TAACTGTTTC ACAGTCCTTA 
CCAGTTCTAA GTACCCTACA GCTGTATTGT TCTTCTGCTT TGGAGAACAC CGTGTCAAAC 
AGGCTTTCGA CAGAGGACTG CTTAATCCCA CTCTATAGTG AAGCTTTACG TTCGTGTAAA 
CAGCATGATG TAAGGCCCTG GATGCAGGCT TTGAGGTATA CAGTATACCA GAGTCAGTTG 
GTGGAAAAAA TCAAAGAACA AACGGTTCCA ATTAGAAGTC ACCTCATGGA ATTGGGTTTA 
ACAGCTGCGA AATTTGCTCG TAAACGTGGA AACGTTGCCC TGGCAACCAG ATTGCTCGCT 
CAATGTAGTG AAGTGCGGTT GGGAAAGACG ACCACTGCTC AAGATTTAGT CCAGTATTTT 
AAAAAACTGT CAACACAAGG TCAAATAGAT GAAAAATGGG GACCTGAACT GGATATTGAG 
AAAACGAAAT TGTTATACAC AGCAGGTCAG TCAACCCATG CGATGGAAAT GCTGAGTTCT 
TGCGCCATAT CCTTCTGCAA GTCTGCCAAA GCTGAATACG CAGTAGCCAA ATCGATTCTT 
ACACTTGCTA AATGGATCCA AGCTGAATGG AAAGAAATTT CAGGACAGTT GAAACAGGTG 
TACAGAGCCC AACAGCAGCA GAACACCACG GGCCTGTCTA CATTATCTAA AAACATACAC 
ACTTTGATCG AACTGCCGTC TGTCAACACC ACAGAAGAGG AGTATCCCCG CATCGAGAGT 
GAATCAACAG TCAACATTGG AGTTGGGGAA CCGGACTTCA TCCTGGGCCA GTTGTATCAC 
CTGTCTTCAG TGCAGGCACC CGAAGTGGCC AAATCTTGGG CAGCGCTGGC AAGTTGGGCT 
TACAGATGGG GAAGAAAGGT GGTGGATAAC GCCAGTCAGG GAGAAGGTGT TCGACTACTG 
CCAAGAGAGA AATCTGAGGT TCAAAATCTG CTTCCAGACA ACATAACAGA GGAGGATCGA 
GAGAAGATCT ACGGCATCCT TGGGCAGGCG GTGTGTCGGC CAGCCGGAAT TCAGGATGAA 
GATATAACAC TGCAGATAAC AGAGAGTGAA GACAATGAGG AAGATGACAT GGTGGATGTG 
ATCTGGCGTC AGTTATTGAC GAGTTGCCCG TGGCTTTCCG ATCTTGATGA AAATGCCACA 
GAAGGCCTAA TTAAAGTGTG GAGAAAAATT GTAGATAGGA TCTTCAGTCT CTACAAACTG 
TCCTGCAGTG CCTATTTTAC TTTCCTTAAA CTCAATGCTG GCCAGGTTAC TCTAGATGAA 
GATGACCCAA GGCTGCACTT GAGCATTAGG TCTGAACAAA GCACTGACGA TGTTATTGTA 
ATGGCAACGT TAAGACTGTT GAGATTGCTG GTCAAACACG CTGGAGAGCT AAGGCAGTAT 
CTGGAGCACG GCCTAGAAAC CACCCCCACT GCTCCTTGGA GAGGTATCAT TCCACAGCTC 
TTCTCCCGTC TAAATCATCC CGAAGTGTAC GTCCGCCAAA GTATTTGTAA TCTACTCTGT 
CGAGTGGCAC AAGATTCTCC TCATCTCATA TTATATCCTG CAATTGTGGG AACTATTTCA 
CTGAGTAGTG AATCGCAGAC ATCAGGCAAT AAGTTGCCTT CAGCCATCCC CACTTTGCTG 
GGCAATATTC AAGGCGAAGA ATTACTCGGA GCGGAATGTG AAGGGGGAAG TCCTCCAGCT 
TCTCAAGACA GCAATAAAGA TGAACCCAAG AATGGCTTAA ATGAAGATCA AGCGATGATG 
CAGGATTGCT ACAGCAAAAT TGTTGATAAA TTGTCTTCTG CCAACCCAAC AATGGTCTTA 
CAGGTTCAAA TGCTGGTGGC CGAGTTGCGC AGGGTGACCG TTCTTTGGGA TGAGCTCTGG 
CTGGGAGTTC TTCTACAACA ACATATGTAT GTTCTGAGGC GCATTCAACA GTTGGAGGAC 
GAAGTAAAGA GAGTCCAGAA TAACAATACG TTACGCAAAG AAGAGAAGAT TGCAATAATG 
CGAGAAAAGC ACACAGCTCT GATGAAGCCC ATCGTATTTG CTTTGGAACA TGTACGGAGC 
ATCACTGCCG CTCCTGCCGA AACACCTCAT GAGAAGTGGT TCCAAGACAA CTATGGTGAG 
GCAATTGAAA ATGCCCTGGA AAAACTCAAG AACCCATTAA ATCCTGCAAA ACCCGGAAGC 
AGTTGGATCC CATTTAAAGA GGTGATGCTA AGTTTGCAGC AGAGAGCACA GAAACGTGCT 
AGTTATATCT TGCGCCTTGA AGAAATCAGC CCCTGGTTGG CTGCCATGAC TAATACTGAA 
ATTGCCCTTC CTGGAGAAGT ATCAGCCAGA GATACCGTGA CGATCCACAG CGTGGGCGGA 
ACCATCACAA TCCTACCTAC TAAAACCAAA CCCAAAAAAC TGCTCTTTCT GGGTTCAGAC 
GGGAAAAACT ACCCCTATCT CTTTAAAGGA CTGGAAGATC TGCACCTGGA TGAGAGAATC 
ATGCAGTTTC TATCTATCGT TAATACCATG TTCGCTACCA TCAATCGCCA AGAGACGCCC 
CGTTTCCATG CCAGGCACTA TTCGGTGACC CCGCTGGGGA CGAGGTCGGG CCTGATTCAG 
TGGGTAGATG GAGCCACTCC CTTATTTGGC CTTTACAAGC GATGGCAACA GCGAGAAGCT 
GCTTTACAAG CGCAAAAGGC CCAGGATTCT TACCAGACTC CTCAAAATCC TGGAATAGTA 
CCTCGGCCCA GCGAGCTTTA TTACAGTAAA ATCGGGCCCG CCCTGAAAGC GGTCGGACTC 
AGTCTCGACG TGTCCCGGCG AGACTGGCCT CTCAACGTGA TGAAGGCTGT GTTGGAAGAA 
CTGATGGAAG CCACCCCTCC AAACCTTCTG GCCAAGGAGC TTTGGTCTTC TTGTACTACA 
CCTGACGAAT GGTGGAGAGT TACCCAGTCT TACGCAAGAT CCACCGCGGT CATGTCAATG 
GTTGGATACA TAATCGGCCT TGGAGATAGA CATCTGGATA ACGTTCTTAT AGACATGACT 
ACAGGAGAAG TTGTTCACAT AGATTACAAT GTTTGTTTTG AAAAAGGTAA AAGTCTGAGA 
GTGCCGGAGA AAGTTCCTTT CAGGATGACT CACAACATCG AAACAGCTCT CGGTGTAACT 
GGAGTTGAGG GAGTTTTCAG GCTTTCTTGT GAACAGGTTC TCCAAATTAT GCGGCGTGGA 
AGAGAGACCC TGCTCACGTT GCTGGAAGCC TTTGTGTACG ATCCTCTTGT GGACTGGACA 
GCCGGAGGGG AAGCAGGATT TGCAGGAGCT GTCTATGGCG GAGGCGGCCA GCAGGCAGAG 
AACAAGCAAA GTAAAAGGGA GATGGAAAGG GACATCACCC GCAGCCTCTT TTCTTCCCGA 
GTAGCTGAAA TCAAGGTGAA CTGGTTTAAA AACAGAGATG AGATGTTGAT TGTGCTTCCA 
AAGCTAGATG GAAGCTTAGA AGAATACCTG AGCCTACAAG AGCAATTGAC AGATGTTGAA 
AAACTGCAAG GCAAACTCGT TGAGGAGATT GACTTTCTAG AAGGAGCCGA AGGAGTAGAT 
CATCCCTCGC ACACTCTGCA GCACAGGTAT TCCGAACACA CCCAACTGCA GACTCAGCAG 
AGAACCGTTC AAGAAGCCAT CCAGGTGAAG TTGAATGAAT TTGAGCAGTG GATAACACAT 
TACCAAGCTG CGTTTAACAA TTTAGAAGCC ACCCAGCTGG CCAGTCTGCT TCAGGAGATT 
AGCACCCAAA TGGACCTGGG TCCTCCAAGC TACGTGCCTG CAACCACCTT CCTGCAGAAC 
GCTGGCCAAG CCCACTTGAT CAGTCAGTGC GAGCAGCTGG AGGGCGAGGT TGGAGCTTTC 
CTTCAGCAGA GGCGCTCCGT CCTGCGAGGC TGTCTCGAGC AGCTGCATAA CTACGCGACC 
GTAGCCCTTC AGTACCCGAA AACCATATTC CAGAAACACC GAATCGAGCA GTGGAAGACC 
TGGATGGAAG AACTTATACG CAATACGACC ATCGAGAGAT GTCAGGAGTT GTACCGGAAA 
TATGAACTCC AGTATGCCCC CCAGCCACCG CCCACGGTGT GTCAGTTCAT AACTGCCACT 
GAAATGACTC TTCAGCGCTA CGCGGCAGAC ATAAATAATA GGCTTATCAG ACAAGTGGAG 
CGCCTGAAAC AAGAAGCAGT GACCGTGCTG GTCTGCGAGG AGCAGTTAAA AGAAATTGAA 
CGGTGTATTA AGGTTTTTCT TCATGAAAAC GGAGAGGAGG GGTCCTGGAG CCTGGCGAGC 
GTTATCATCT CTGCACTCTG TGCACTTACA AGGCGCAACT TAATGATGGA AGGTGCCGCT 
TCTAGTGCTG GCGAGCAGCT GGTCGATCTG ACTTCCCGAG ATGGGGCCTG GTTCTTGGAG 
GAGCTGTGCA GCATGAGTGG AAATGTAACC TGCCTTGTAC AGTTACTGAA GCAGTGTCAC 
CTGGTCCCTC AGGATTTAGA TATTCCCAAT CCCCTCGAAG CTTCGGAGGC AGTTCACTTA 
GCTAATGGAG TCTACACCTC GCTTCAGGAA TTGAACTCCA ACTTCCGGCA GATCATCTTT 
CCGGAAGCTC TGCGATGTTT AATGAAAGGG GAATACACTT TAGAAAGCAT GCTGCACGAA 
CTGGACAACC TCATTGAACA GACAACTGAT GGAGTTCCGT TGCAAACCCT GGTTGACTCT 
CTTCAGGCCT ATTTAAGAAA TGCAGCTATG GGCCTGGAAG AAGAGACACA TTCTCACTAT 
ATTGATGTTG CCAGAATACT CCATTCTCAG TATGGCGAAT TGATTCAACC AAGGAATGGT 
TCAGTTGATG AAACACCAAA AATGTCCGCC GGTCAAATGC TTTTGGTTGC GTTCGATGGA 
ATGTTCGCCC AAGTGGAAAC CGCTTTTGGC TTGTTAATTG AAAAGTTAAA CAAGATGGAA 
GTCCCCGTAT CGTGGCGAAA GATAGACATC ATAAGAGAAG CCAGAAGCAC CCAGATTAAT 
TTCTTTGACG ACGATAATCA CCGACAGGTT CTGGAGGAGA TTTTCTTTCT CAAAAGACTG 
CAGACCATCA AAGAATTCTT TAGACTCTGT GGTACTTTTT CTAAAACGCT GTCAGGATCG 
AGTTCGCTCG AAGATCAGAG CCCGGTGAAT GGACCCGTTC AAATTGTTAA TGTGAAAACG 
CTTTTCAGAA ACTCTTGTTT TAGTGAAGAT CAGATGGCCA AACCCATAAA GGCGTTCACA 
GCTGACTTCG TGAGGCAGCT CTTAATTGGC CTGCCTAATC AAGCCTTGGG GCTAACCCTT 
TGCAGTTTCA TCAGCGCGCT GGGCGTCGAC ATCATCGCCC AGGTCGAGGC AAAGGACTTT 
GGGGCAGAGA GCAAGGTTTC GGTGGACGAT CTGTGTAAGA AAGCCGTGGA GCACAACATC 
CAGATTGGAA AGTTCTCCCA GCTGGTCATG AACCGAGCCA CGGTCCTGGC CAGTTCCTAC 
GACACTGCTT GGAAGAAGCA CGATTTGGTG CGCAGGCTAG AAACCAGTAT TTCCTCTTTC 
AAGACCAGCC TGCAGAGAGT GCAGCTCCAG ATCGCCATGT TTCAGTGGCA GCACGAAGAT 
CTCCTAATCA CCCGACCACA AGCCATGTCT GTGACTCCTC CACCTCGCTC TGCTATTCTA 
ACAAATATGA AAAAGAAGCT GCATACCCTG AGCCAGATCG AAACGTCCAT TGCAGCGGTT 
CAGGAGAAAC TAGCAGCCCT CGAAGCAAGC ATCGAACAAC GTTTGAAGTG GGCAGGAGGG 
GCCAATCCCG CATTGGCTCC AGTCCTACAA GATTTTGAGG CAACAATAGC GGAAAGAAGG 
AATCTCGTTC TGAAAGAAAG CCAACGAGCA AATCAGGTCA CTTTCCTCTG CAGCAATATC 
ATTCATTTTG AAAGCCTACG AACGCGAACT GCCGAAGCTC TAAATCTCGA TGCTGCCTTG 
TTTGAACTCA TCAAGCGCTG TCAACAGATG TGTTCGTTTG CCTCACAGTT TAACAGCTCG 
GTTTCTGAAC TGGAACTGCG TTTACTGCAA AGAGTGGGTT CCGGTCTCGA ACCTCCCATT 
GGCAGCCCTG AATGGCTTGT TTCTGCACAC AAACAACTGA CCCAGGATAT GTCTACTCAG 
AGGGCGGTTC AAACAGAAAA AGAACAGCAA ATCGAATCAG TCTGTGAAGC AATTCAGAAC 
CTTGTGGATA GTATTAAGAC AGTGCTTACC GGTCACAACA GGCAACTTGG AGATGTTAAA 
CATCTATTGA AAGCAATGGC CAAGGATGAA GAGGCTGCTT TGGCAGATGG TGAAGATGTT 
CCCTATGAGA ACAGTGTTAG ACAATTCTTG GGTGAATATA AGTCATGGCA AGACAATATT 
CAAACGGTAT TGTTTACATT AGTTCAGGCT ATGGGGCAGG TTCGCAGCCA AGAGCATGTG 
GAGATGCTGC AGGAGATAAC TCCCACCTTG AAAGAGCTGA AAACACAAAG CCAGAGTATC 
TACAATAATC TAGTGAGTTT TGCATCACCC TTAGTCACAG ATGCAACAAA TGAGTGTTCC 
AGCCCAACAT CATCTGCTAC GTATCAGCCA ACCTTTGCTG CAGCGGTCCG CAGTAACACT 
GGCCAGAAGA CTCAGCCTGA AGTTATGTCG CAGAATGCAA GGAAGCTGAT TCAGAAAAAT 
CTTGCAACAT CAGCAGATAC CCCACCAAGC ACAGTTCCAG GAACTGGCAA GAGTGTGGCC 
TGTAGTCCTA AAAAAGCAGT CAGGGATCCA AAAACTGGAA AAGCTGTGCA AGAAAGGAAC 
TCCTATGCAG TGAGTGTGTG GAAGCGAGTC AAAGCCAAGT TAGAGGGCCG AGATGTGGAT 
CCGAACAGGA GGATGTCTGT TGCTGAACAG GTGGACTATG TGATTAAGGA AGCAACTAAT 
CTAGATAACT TGGCTCAGCT GTATGAAGGT TGGACGGCCT GGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028913887.1
CDS	116..11023
Translation	MRFLRTDSAPADQDHLKYSSSRDRGSSASYGLQPSNSVVVSRQRHDDIRVHTDIQNEEKGGYNVNGGAGENTYGRKSLGQELRVNNVTNPDFTSVQHGNRALATKDMRKSQERSLSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKQVLVKQLDNILTAIHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSTKDEVKLLYLCATFKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGHPITEAYVTDVLYRVMRCVTTANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPASKLLLLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHNLQLPEACSEIQHDAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLLEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNSNHTEIQEISLAIRSHMSKTPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNSISQGNELEVIIMMVVEALCELHCPEAIQGLAVWSSAVVGKSLVWINSVAQQAEGRFEKATVEYQEHLCAMTGVDCCITGFDKSILKLANMNTNNTSPKHSLNGDARKTVLAKPSDSSPEVINYLGNKACECYISLADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFLECTEQLELLPGENINLLASGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATAVSHIEQEQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLYSEALRSCKQHDVRPWMQALRYTVYQSQLVEKIKEQTVPIRSHLMELGLTAAKFARKRGNVALATRLLAQCSEVRLGKTTTAQDLVQYFKKLSTQGQIDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNTTGLSTLSKNIHTLIELPSVNTTEEEYPRIESESTVNIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDNITEEDREKIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLLTSCPWLSDLDENATEGLIKVWRKIVDRIFSLYKLSCSAYFTFLKLNAGQVTLDEDDPRLHLSIRSEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQTSGNKLPSAIPTLLGNIQGEELLGAECEGGSPPASQDSNKDEPKNGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGEAIENALEKLKNPLNPAKPGSSWIPFKEVMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKNYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLNVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGVFRLSCEQVLQIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLIVLPKLDGSLEEYLSLQEQLTDVEKLQGKLVEEIDFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRTVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATTFLQNAGQAHLISQCEQLEGEVGAFLQQRRSVLRGCLEQLHNYATVALQYPKTIFQKHRIEQWKTWMEELIRNTTIERCQELYRKYELQYAPQPPPTVCQFITATEMTLQRYAADINNRLIRQVERLKQEAVTVLVCEEQLKEIERCIKVFLHENGEEGSWSLASVIISALCALTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPLEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDNLIEQTTDGVPLQTLVDSLQAYLRNAAMGLEEETHSHYIDVARILHSQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLIEKLNKMEVPVSWRKIDIIREARSTQINFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQSPVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSFKTSLQRVQLQIAMFQWQHEDLLITRPQAMSVTPPPRSAILTNMKKKLHTLSQIETSIAAVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRANQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVGSGLEPPIGSPEWLVSAHKQLTQDMSTQRAVQTEKEQQIESVCEAIQNLVDSIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPTFAAAVRSNTGQKTQPEVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_029058054.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029058054.1

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCACCA GCCGACCAGG ATCATTTAAA ATATTCTTCG 
TCTAGAGATC GGGGCAGTTC TGCCTCTTAT GGATTACAAC CTTCAAATTC AGTTGTGGTG 
TCTCGTCAAC GGCACGATGA TATCAGAGTC CACACCGACA TACAGAATGA AGAAAAGGGT 
GGCTACAATG TCAATGGAGG AGCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAACCCT GATTTCACCA GTGTTCAGCA TGGAAATCGT 
GCTTTAGCCA CAAAAGACAT GAGGAAATCA CAGGAGCGAT CGTTGTCTTA CTCTGACGAG 
TCTCGACTGT CAAACCTTCT TCGGAGGATT ACCCGGGAAG ACGACAGAGA CCGAAGACTG 
GCTACTGTGA AGCAATTGAA AGAATTCATT CAGCAACCAG AAAATAAGCA GGTCTTAGTT 
AAACAACTGG ATAATATCCT GACCGCTATA CATGATGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAAT TGAGGCAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTTTGAGT 
TATGAGGCCG AGAAGATCTT CAAATGGATT TTTAGCAAAT TTAGCTCGTC CACAAAAGAT 
GAAGTTAAAC TGCTTTACTT ATGTGCCACC TTCAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCCAT TCTAGAAAAT 
GTGGATACAC CAGAATTGCT ATGCAAATGT GTGAAGTGCA TTCTTCTGGT GGCCCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACGGTTG ACATATTGGT TGGGTGGCAT 
ATAGATCACA CTCAGAAACC TTCCTTGACA CAGCAAGTGT CTGGATGGCT GCAGAGTCTG 
GAGCCCTTTT GGGTAGCCGA TCTTGCATTT TCCACCACCC TTCTCGGTCA GTTTCTGGAA 
GATATGGAGG CTTATGCTGA AGATCTCAGT CATGTGGCAT CTGGGGAATC GGTCGATGAA 
GACATTCCTC CTCCATCAGT GTCCTTACCG AAGTTGGCGG CTCTTCTCCG GGTTTTCAGT 
ACTGTGGTGC GGAGTATTGG GGAGCGCTTC AGTCCAATTA GAGGACATCC AATTACCGAG 
GCTTATGTAA CAGATGTTTT GTACAGAGTA ATGAGATGTG TGACAACAGC AAACCAAGTA 
TTTTTTTCTG AAGCTGTATT GACAGCTGCC AATGAATGTG TCGGTGTATT GCTTGGTAGC 
CTAGATCCTA GTATGACTAT TCATTGTGAC ATGGTCATCA CCTATGGACT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACAGATTAT ATCATCTCGG TCTTAAATTT ACTGACGTTG 
ATTGTTGAGC AGATCAATAC AAAGCTGCCA TCGTCATTCG TCGAAAAACT GTTTATACCA 
GCGTCTAAAC TACTGCTTCT GCGCTATCAT AAAGAAAAAG AGGTTGTTGC AGTAGCTCAT 
GCTGTATATC AAGCCGTTCT TAGCCTGAAG AACATTCCCG TCTTGGAGAC CGCCTATAAG 
TTAATTTTAG GAGAAATGAC TTGTGCACTG AATAATCTCC TGCATAACCT TCAACTTCCT 
GAGGCCTGTT CCGAAATCCA ACACGATGCT TTTAAGAATC ACGTTTTCAA TGTGGACAAT 
GCCAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACCA CGATTGGAAA TGCGAAAAAC 
TCATTAATTG GGATGTGGGC ACTCTCGCCA ACCGTTTTTG CACTTCTGAG CAAGAATCTG 
ATGATAGTGC ATGGAGATCT CGCCGTTCAC TTCCCAGCTA TTCAGTATGC CGTTCTCTAC 
ACTTTGTATT CACATTGTAC CAGACATGAT CACTTCATTT CTAGCAGTCT TAGCTCCTCT 
TCTCCTTCTC TCTTTGATGG AGCAGTTATC AGCACTGTCA CCACAGCTAC AAAGAAGCAT 
TTCTCAATCA TATTAAACCT CCTGGGAATA TTACTGAAGA AAGATAATCT TAATCAGGAT 
ACCAGGAAAC TACTAATGAC CTGGGCTTTG GAAGTTGCTG TTCTAATGAA AAAGTCTGAA 
ACATATGCAC CTTTGTTCTC TCTTCCGTCG TTCCATAAAT TTTGCAAAGG ACTTCTAGCA 
AACACTCTTC TTGAAGATGT GAATATCTGC CTTCAAGCAT GTAGTAGTCT GCATGCCTTG 
TCATCTTCCC TGCCAGATGA CCTTTTACAA AGGTGTGTTG ATGTGTGCCG CGTTCAGCTG 
GTCCATAGTG GAACTCGGAT TCGACAAGCC TTTGGAAAGC TGTTGAAGTC TATTCCGTTA 
GATGTTGTCT TGAGCAATAG CAACCACACA GAGATTCAAG AAATTTCCTT AGCTATAAGG 
AGTCACATGA GCAAAACTCC AAGTAATACT TTCCATCCAC AAGATTTCTC TGACGTGATA 
AGTTTTATTT TGTATGGAAA CTCTCATAGA ACCGGGAAGG ACAATTGGTT AGAAAGATTG 
TTCTACAGCT GTCAGAGATT GGATAAGCGT GATCAGTCAA CCATTCCACG CAATCTACTC 
AAAACAGATG CCGTCCTCTG GCAGTGGGCT ATTTGGGAAG CTGCTCAGTT CACTGTTCTC 
TCTAAACTAA GAACCCCGCT GGGCAGAGCT CAAGATACCT TCCAGACAAT CGAAGGTATC 
ATTAGAAGTC TTGCCGCTCA TACATTAAAC CCCGATCAAG ATGTTAGCCA GTGGACCACT 
GCGGACAATG ATGAAGGACA TAGCAGCAAC CAACTCAGAC TTGTTCTTCT ACTGCAGTAC 
TTGGAAAATT TGGAAAAACT AATGTATAAT GCATATGAAG GTTGTGCTAA TGCACTGACT 
TCCCCTCCTA AGGTTATCAG GACATTTTTC TATACCAATC GCCAGACATG TCAGGACTGG 
TTAACTCGGA TTAGACTTTC CATCATGAGA GTTGGATTGT TGGCCGGTCA GCCCGCTGTG 
ACAGTAAGAC ACGGTTTTGA TTTGCTCACC GAAATGAAGA CCAATAGTAT CTCTCAGGGA 
AATGAGCTAG AAGTGATTAT TATGATGGTC GTAGAAGCAC TTTGTGAGCT TCACTGTCCT 
GAAGCTATAC AGGGATTAGC TGTCTGGTCT TCAGCAGTGG TTGGAAAGAG TCTCGTCTGG 
ATTAATTCAG TGGCACAGCA AGCCGAAGGG CGGTTTGAAA AGGCAACTGT TGAATACCAG 
GAGCACTTGT GTGCTATGAC AGGGGTAGAT TGCTGCATAA CTGGGTTTGA TAAATCCATT 
CTGAAGTTGG CTAACATGAA TACTAACAAT ACCAGCCCCA AACATTCTCT CAATGGTGAC 
GCTAGGAAAA CTGTTCTGGC CAAACCAAGT GATTCTTCTC CTGAGGTTAT TAATTACTTG 
GGTAACAAAG CATGTGAGTG CTACATCTCT CTTGCTGATT GGGCTGCTGT GCAGGAATGG 
CAGAATGCTG TCCATGACTT GAAAAAGAGC ACAAGTAGTA CTTCCCTCAA TTTAAAAGCT 
GACTTTAACT ACATAAAATC ACTGAGCAGC TTTGAATCTG GCGAATTTCT CGAATGTACA 
GAACAACTAG AATTGCTGCC AGGAGAAAAC ATTAACTTAC TTGCCAGCGG GTCAAAGGAA 
AAAATAGATA TGAAAAAGCT ACTTCCAAAC ATGTTAAGCC CAGATCCACG GGAACTTCAG 
AAATCCATTG AAGTTCAGTT GCTGAGAAGT TCCGTTTGTT TGGCTACCGC TGTAAGCCAC 
ATAGAGCAGG AACAAAAGTG GCAATCTATA ACTGAAAACG TTGTAAAATA TTTGAAGCAA 
ACATCAAGAA TAGCTATTGG ACCACTGAGA CTTTCCACTT TAACTGTTTC ACAGTCCTTA 
CCAGTTCTAA GTACCCTACA GCTGTATTGT TCTTCTGCTT TGGAGAACAC CGTGTCAAAC 
AGGCTTTCGA CAGAGGACTG CTTAATCCCA CTCTATAGTG AAGCTTTACG TTCGTGTAAA 
CAGCATGATG TAAGGCCCTG GATGCAGGCT TTGAGGTATA CAGTATACCA GAGTCAGTTG 
GTGGAAAAAA TCAAAGAACA AACGGTTCCA ATTAGAAGTC ACCTCATGGA ATTGGGTTTA 
ACAGCTGCGA AATTTGCTCG TAAACGTGGA AACGTTGCCC TGGCAACCAG ATTGCTCGCT 
CAATGTAGTG AAGTGCGGTT GGGAAAGACG ACCACTGCTC AAGATTTAGT CCAGTATTTT 
AAAAAACTGT CAACACAAGG TCAAATAGAT GAAAAATGGG GACCTGAACT GGATATTGAG 
AAAACGAAAT TGTTATACAC AGCAGGTCAG TCAACCCATG CGATGGAAAT GCTGAGTTCT 
TGCGCCATAT CCTTCTGCAA GTCTGCCAAA GCTGAATACG CAGTAGCCAA ATCGATTCTT 
ACACTTGCTA AATGGATCCA AGCTGAATGG AAAGAAATTT CAGGACAGTT GAAACAGGTG 
TACAGAGCCC AACAGCAGCA GAACACCACG GGCCTGTCTA CATTATCTAA AAACATACAC 
ACTTTGATCG AACTGCCGTC TGTCAACACC ACAGAAGAGG AGTATCCCCG CATCGAGAGT 
GAATCAACAG TCAACATTGG AGTTGGGGAA CCGGACTTCA TCCTGGGCCA GTTGTATCAC 
CTGTCTTCAG TGCAGGCACC CGAAGTGGCC AAATCTTGGG CAGCGCTGGC AAGTTGGGCT 
TACAGATGGG GAAGAAAGGT GGTGGATAAC GCCAGTCAGG GAGAAGGTGT TCGACTACTG 
CCAAGAGAGA AATCTGAGGT TCAAAATCTG CTTCCAGACA ACATAACAGA GGAGGATCGA 
GAGAAGATCT ACGGCATCCT TGGGCAGGCG GTGTGTCGGC CAGCCGGAAT TCAGGATGAA 
GATATAACAC TGCAGATAAC AGAGAGTGAA GACAATGAGG AAGATGACAT GGTGGATGTG 
ATCTGGCGTC AGTTATTGAC GAGTTGCCCG TGGCTTTCCG ATCTTGATGA AAATGCCACA 
GAAGGCCTAA TTAAAGTGTG GAGAAAAATT GTAGATAGGA TCTTCAGTCT CTACAAACTG 
TCCTGCAGTG CCTATTTTAC TTTCCTTAAA CTCAATGCTG GCCAGGTTAC TCTAGATGAA 
GATGACCCAA GGCTGCACTT GAGCATTAGG TCTGAACAAA GCACTGACGA TGTTATTGTA 
ATGGCAACGT TAAGACTGTT GAGATTGCTG GTCAAACACG CTGGAGAGCT AAGGCAGTAT 
CTGGAGCACG GCCTAGAAAC CACCCCCACT GCTCCTTGGA GAGGTATCAT TCCACAGCTC 
TTCTCCCGTC TAAATCATCC CGAAGTGTAC GTCCGCCAAA GTATTTGTAA TCTACTCTGT 
CGAGTGGCAC AAGATTCTCC TCATCTCATA TTATATCCTG CAATTGTGGG AACTATTTCA 
CTGAGTAGTG AATCGCAGAC ATCAGGCAAT AAGTTGCCTT CAGCCATCCC CACTTTGCTG 
GGCAATATTC AAGGCGAAGA ATTACTCGGA GCGGAATGTG AAGGGGGAAG TCCTCCAGCT 
TCTCAAGACA GCAATAAAGA TGAACCCAAG AATGGCTTAA ATGAAGATCA AGCGATGATG 
CAGGATTGCT ACAGCAAAAT TGTTGATAAA TTGTCTTCTG CCAACCCAAC AATGGTCTTA 
CAGGTTCAAA TGCTGGTGGC CGAGTTGCGC AGGGTGACCG TTCTTTGGGA TGAGCTCTGG 
CTGGGAGTTC TTCTACAACA ACATATGTAT GTTCTGAGGC GCATTCAACA GTTGGAGGAC 
GAAGTAAAGA GAGTCCAGAA TAACAATACG TTACGCAAAG AAGAGAAGAT TGCAATAATG 
CGAGAAAAGC ACACAGCTCT GATGAAGCCC ATCGTATTTG CTTTGGAACA TGTACGGAGC 
ATCACTGCCG CTCCTGCCGA AACACCTCAT GAGAAGTGGT TCCAAGACAA CTATGGTGAG 
GCAATTGAAA ATGCCCTGGA AAAACTCAAG AACCCATTAA ATCCTGCAAA ACCCGGAAGC 
AGTTGGATCC CATTTAAAGA GGTGATGCTA AGTTTGCAGC AGAGAGCACA GAAACGTGCT 
AGTTATATCT TGCGCCTTGA AGAAATCAGC CCCTGGTTGG CTGCCATGAC TAATACTGAA 
ATTGCCCTTC CTGGAGAAGT ATCAGCCAGA GATACCGTGA CGATCCACAG CGTGGGCGGA 
ACCATCACAA TCCTACCTAC TAAAACCAAA CCCAAAAAAC TGCTCTTTCT GGGTTCAGAC 
GGGAAAAACT ACCCCTATCT CTTTAAAGGA CTGGAAGATC TGCACCTGGA TGAGAGAATC 
ATGCAGTTTC TATCTATCGT TAATACCATG TTCGCTACCA TCAATCGCCA AGAGACGCCC 
CGTTTCCATG CCAGGCACTA TTCGGTGACC CCGCTGGGGA CGAGGTCGGG CCTGATTCAG 
TGGGTAGATG GAGCCACTCC CTTATTTGGC CTTTACAAGC GATGGCAACA GCGAGAAGCT 
GCTTTACAAG CGCAAAAGGC CCAGGATTCT TACCAGACTC CTCAAAATCC TGGAATAGTA 
CCTCGGCCCA GCGAGCTTTA TTACAGTAAA ATCGGGCCCG CCCTGAAAGC GGTCGGACTC 
AGTCTCGACG TGTCCCGGCG AGACTGGCCT CTCAACGTGA TGAAGGCTGT GTTGGAAGAA 
CTGATGGAAG CCACCCCTCC AAACCTTCTG GCCAAGGAGC TTTGGTCTTC TTGTACTACA 
CCTGACGAAT GGTGGAGAGT TACCCAGTCT TACGCAAGAT CCACCGCGGT CATGTCAATG 
GTTGGATACA TAATCGGCCT TGGAGATAGA CATCTGGATA ACGTTCTTAT AGACATGACT 
ACAGGAGAAG TTGTTCACAT AGATTACAAT GTTTGTTTTG AAAAAGGTAA AAGTCTGAGA 
GTGCCGGAGA AAGTTCCTTT CAGGATGACT CACAACATCG AAACAGCTCT CGGTGTAACT 
GGAGTTGAGG GAGTTTTCAG GCTTTCTTGT GAACAGGTTC TCCAAATTAT GCGGCGTGGA 
AGAGAGACCC TGCTCACGTT GCTGGAAGCC TTTGTGTACG ATCCTCTTGT GGACTGGACA 
GCCGGAGGGG AAGCAGGATT TGCAGGAGCT GTCTATGGCG GAGGCGGCCA GCAGGCAGAG 
AACAAGCAAA GTAAAAGGGA GATGGAAAGG GACATCACCC GCAGCCTCTT TTCTTCCCGA 
GTAGCTGAAA TCAAGGTGAA CTGGTTTAAA AACAGAGATG AGATGTTGAT TGTGCTTCCA 
AAGCTAGATG GAAGCTTAGA AGAATACCTG AGCCTACAAG AGCAATTGAC AGATGTTGAA 
AAACTGCAAG GCAAACTCGT TGAGGAGATT GACTTTCTAG AAGGAGCCGA AGGAGTAGAT 
CATCCCTCGC ACACTCTGCA GCACAGGTAT TCCGAACACA CCCAACTGCA GACTCAGCAG 
AGAACCGTTC AAGAAGCCAT CCAGGTGAAG TTGAATGAAT TTGAGCAGTG GATAACACAT 
TACCAAGCTG CGTTTAACAA TTTAGAAGCC ACCCAGCTGG CCAGTCTGCT TCAGGAGATT 
AGCACCCAAA TGGACCTGGG TCCTCCAAGC TACGTGCCTG CAACCACCTT CCTGCAGAAC 
GCTGGCCAAG CCCACTTGAT CAGTCAGTGC GAGCAGCTGG AGGGCGAGGT TGGAGCTTTC 
CTTCAGCAGA GGCGCTCCGT CCTGCGAGGC TGTCTCGAGC AGCTGCATAA CTACGCGACC 
GTAGCCCTTC AGTACCCGAA AACCATATTC CAGAAACACC GAATCGAGCA GTGGAAGACC 
TGGATGGAAG AACTTATACG CAATACGACC ATCGAGAGAT GTCAGGAGTT GTACCGGAAA 
TATGAACTCC AGTATGCCCC CCAGCCACCG CCCACGGTGT GTCAGTTCAT AACTGCCACT 
GAAATGACTC TTCAGCGCTA CGCGGCAGAC ATAAATAATA GGCTTATCAG ACAAGTGGAG 
CGCCTGAAAC AAGAAGCAGT GACCGTGCTG GTCTGCGAGG AGCAGTTAAA AGAAATTGAA 
CGGTGTATTA AGGTTTTTCT TCATGAAAAC GGAGAGGAGG GGTCCTGGAG CCTGGCGAGC 
GTTATCATCT CTGCACTCTG TGCACTTACA AGGCGCAACT TAATGATGGA AGGTGCCGCT 
TCTAGTGCTG GCGAGCAGCT GGTCGATCTG ACTTCCCGAG ATGGGGCCTG GTTCTTGGAG 
GAGCTGTGCA GCATGAGTGG AAATGTAACC TGCCTTGTAC AGTTACTGAA GCAGTGTCAC 
CTGGTCCCTC AGGATTTAGA TATTCCCAAT CCCCTCGAAG CTTCGGAGGC AGTTCACTTA 
GCTAATGGAG TCTACACCTC GCTTCAGGAA TTGAACTCCA ACTTCCGGCA GATCATCTTT 
CCGGAAGCTC TGCGATGTTT AATGAAAGGG GAATACACTT TAGAAAGCAT GCTGCACGAA 
CTGGACAACC TCATTGAACA GACAACTGAT GGAGTTCCGT TGCAAACCCT GGTTGACTCT 
CTTCAGGCCT ATTTAAGAAA TGCAGCTATG GGCCTGGAAG AAGAGACACA TTCTCACTAT 
ATTGATGTTG CCAGAATACT CCATTCTCAG TATGGCGAAT TGATTCAACC AAGGAATGGT 
TCAGTTGATG AAACACCAAA AATGTCCGCC GGTCAAATGC TTTTGGTTGC GTTCGATGGA 
ATGTTCGCCC AAGTGGAAAC CGCTTTTGGC TTGTTAATTG AAAAGTTAAA CAAGATGGAA 
GTCCCCGTAT CGTGGCGAAA GATAGACATC ATAAGAGAAG CCAGAAGCAC CCAGATTAAT 
TTCTTTGACG ACGATAATCA CCGACAGGTT CTGGAGGAGA TTTTCTTTCT CAAAAGACTG 
CAGACCATCA AAGAATTCTT TAGACTCTGT GGTACTTTTT CTAAAACGCT GTCAGGATCG 
AGTTCGCTCG AAGATCAGAG CCCGGTGAAT GGACCCGTTC AAATTGTTAA TGTGAAAACG 
CTTTTCAGAA ACTCTTGTTT TAGTGAAGAT CAGATGGCCA AACCCATAAA GGCGTTCACA 
GCTGACTTCG TGAGGCAGCT CTTAATTGGC CTGCCTAATC AAGCCTTGGG GCTAACCCTT 
TGCAGTTTCA TCAGCGCGCT GGGCGTCGAC ATCATCGCCC AGGTCGAGGC AAAGGACTTT 
GGGGCAGAGA GCAAGGTTTC GGTGGACGAT CTGTGTAAGA AAGCCGTGGA GCACAACATC 
CAGATTGGAA AGTTCTCCCA GCTGGTCATG AACCGAGCCA CGGTCCTGGC CAGTTCCTAC 
GACACTGCTT GGAAGAAGCA CGATTTGGTG CGCAGGCTAG AAACCAGTAT TTCCTCTTTC 
AAGACCAGCC TGCAGAGAGT GCAGCTCCAG ATCGCCATGT TTCAGTGGCA GCACGAAGAT 
CTCCTAATCA CCCGACCACA AGCCATGTCT GTGACTCCTC CACCTCGCTC TGCTATTCTA 
ACAAATATGA AAAAGAAGCT GCATACCCTG AGCCAGATCG AAACGTCCAT TGCAGCGGTT 
CAGGAGAAAC TAGCAGCCCT CGAAGCAAGC ATCGAACAAC GTTTGAAGTG GGCAGGAGGG 
GCCAATCCCG CATTGGCTCC AGTCCTACAA GATTTTGAGG CAACAATAGC GGAAAGAAGG 
AATCTCGTTC TGAAAGAAAG CCAACGAGCA AATCAGGTCA CTTTCCTCTG CAGCAATATC 
ATTCATTTTG AAAGCCTACG AACGCGAACT GCCGAAGCTC TAAATCTCGA TGCTGCCTTG 
TTTGAACTCA TCAAGCGCTG TCAACAGATG TGTTCGTTTG CCTCACAGTT TAACAGCTCG 
GTTTCTGAAC TGGAACTGCG TTTACTGCAA AGAGTGGGTT CCGGTCTCGA ACCTCCCATT 
GGCAGCCCTG AATGGCTTGT TTCTGCACAC AAACAACTGA CCCAGGATAT GTCTACTCAG 
AGGGCGGTTC AAACAGAAAA AGAACAGCAA ATCGAATCAG TCTGTGAAGC AATTCAGAAC 
CTTGTGGATA GTATTAAGAC AGTGCTTACC GGTCACAACA GGCAACTTGG AGATGTTAAA 
CATCTATTGA AAGCAATGGC CAAGGATGAA GAGGCTGCTT TGGCAGATGG TGAAGATGTT 
CCCTATGAGA ACAGTGTTAG ACAATTCTTG GGTGAATATA AGTCATGGCA AGACAATATT 
CAAACGGTAT TGTTTACATT AGTTCAGGCT ATGGGGCAGG TTCGCAGCCA AGAGCATGTG 
GAGATGCTGC AGGAGATAAC TCCCACCTTG AAAGAGCTGA AAACACAAAG CCAGAGTATC 
TACAATAATC TAGTGAGTTT TGCATCACCC TTAGTCACAG ATGCAACAAA TGAGTGTTCC 
AGCCCAACAT CATCTGCTAC GTATCAGCCA ACCTTTGCTG CAGCGGTCCG CAGTAACACT 
GGCCAGAAGA CTCAGCCTGA AGTTATGTCG CAGAATGCAA GGAAGCTGAT TCAGAAAAAT 
CTTGCAACAT CAGCAGATAC CCCACCAAGC ACAGTTCCAG GAACTGGCAA GAGTGTGGCC 
TGTAGTCCTA AAAAAGCAGT CAGGGATCCA AAAACTGGAA AAGCTGTGCA AGAAAGGAAC 
TCCTATGCAG TGAGTGTGTG GAAGCGAGTC AAAGCCAAGT TAGAGGGCCG AGATGTGGAT 
CCGAACAGGA GGATGTCTGT TGCTGAACAG GTGGACTATG TGATTAAGGA AGCAACTAAT 
CTAGATAACT TGGCTCAGCT GTATGAAGGT TGGACGGCCT GGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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1-732-210-0262

SMG1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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