ASAH1 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASAH1
Entrez Gene ID	100171626
Full Name	N-acylsphingosine amidohydrolase 1
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001131302.1	NP_001124774.1	acid ceramidase precursor

Pathways from BioSystems
Gene Ontology

The following ASAH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASAH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSa22184	NM_001131302.1 Latest version!	Pongo abelii N-acylsphingosine amidohydrolase 1 (ASAH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASAH1 ( NM_001131302.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> NP_001124774.1 Pongo abelii N-acylsphingosine amidohydrolase 1 (ASAH1), mRNA.

Price & Availability↑

CloneID	OSa22184
Clone ID Related Accession (Same CDS sequence)	NM_001131302.1
Accession Version	NM_001131302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Pongo abelii(Sumatran orangutan)
Product	acid ceramidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR857415.1. ##Evidence-Data-START## Transcript exon combination :: CR857415.1, CR858538.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTGGGCC GGAGTCGCGT CGCTTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA 
CCAACGTACA GAGGTCCAGT TCCATGGTAC ACCATAAATC TTGATTTACC ACCCTACAAA 
AGATGGCATG AATTGATGGT TGACAAGGCA CCAATGCTAA AGGTTATAGT GAATTCTCTG 
AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTG TGCAGGTGGT GGATGAAAAA 
TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTGTTGCC 
GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT 
ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA CGGGAGAAAC 
ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG 
CAACTAAAAC CTCTAACAGT GAATTTGGAC TTCCGAAGAA ACAACAAAAC TGTCTTCAAG 
GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT 
CTTACACTGA ATGAACGCTT CAGTATAAAT GGTGGTTATC TGGGTGTTCT AGAATGGATT 
TTGGGAAAGA AAGATGCCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC 
ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC 
TTTATCCTGG GAGGCAACCA GTCGGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA 
TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGGT GGTATGTGGT ACAAACAAAT 
TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC CAAGATGTGT 
CTGAACCGCA CCACCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA 
AAACCTGTCC TCAACAAGCT GACCGTATTC ACAACCTTGA TAGATGTTAC CAAAGGTCAA 
TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124774.1
CDS	37..1224
Translation	MLGRSRVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGPVPWYTINLDLPPYKRWHELMVDKAPMLKVIVNSLKNMINTFVPSGKIVQVVDEKLPGLLGNFPGPFEEEMKGVAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFRRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGVLEWILGKKDAMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTTQENISFETMYDVLSTKPVLNKLTVFTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_001131302.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii N-acylsphingosine amidohydrolase 1 (ASAH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001131302.1

ORF Insert Sequence:

ATGCTGGGCC GGAGTCGCGT CGCTTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA 
CCAACGTACA GAGGTCCAGT TCCATGGTAC ACCATAAATC TTGATTTACC ACCCTACAAA 
AGATGGCATG AATTGATGGT TGACAAGGCA CCAATGCTAA AGGTTATAGT GAATTCTCTG 
AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTG TGCAGGTGGT GGATGAAAAA 
TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTGTTGCC 
GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT 
ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA CGGGAGAAAC 
ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG 
CAACTAAAAC CTCTAACAGT GAATTTGGAC TTCCGAAGAA ACAACAAAAC TGTCTTCAAG 
GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT 
CTTACACTGA ATGAACGCTT CAGTATAAAT GGTGGTTATC TGGGTGTTCT AGAATGGATT 
TTGGGAAAGA AAGATGCCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC 
ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC 
TTTATCCTGG GAGGCAACCA GTCGGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA 
TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGGT GGTATGTGGT ACAAACAAAT 
TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC CAAGATGTGT 
CTGAACCGCA CCACCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA 
AAACCTGTCC TCAACAAGCT GACCGTATTC ACAACCTTGA TAGATGTTAC CAAAGGTCAA 
TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GGTGGTGA