TTF2 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTF2
Entrez Gene ID	100436073
Full Name	transcription termination factor 2
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024234473.1	XP_024090241.1	transcription termination factor 2 isoform X2
XM_002810380.3	XP_002810426.2	transcription termination factor 2
XM_024234466.1	XP_024090234.1	transcription termination factor 2 isoform X1
XM_024234463.1	XP_024090231.1	transcription termination factor 2 isoform X1

Pathways from BioSystems
Gene Ontology

The following TTF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa163440	XM_024234463.1 Latest version!	Pongo abelii transcription termination factor 2 (TTF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa163440	XM_024234466.1 Latest version!	Pongo abelii transcription termination factor 2 (TTF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa163441	XM_024234473.1 Latest version!	Pongo abelii transcription termination factor 2 (TTF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa01319	XM_002810380.3 Latest version!	Pongo abelii transcription termination factor, RNA polymerase II (TTF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTF2 ( XM_024234466.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024090234.1 Pongo abelii transcription termination factor 2 (TTF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa163440
Clone ID Related Accession (Same CDS sequence)	XM_024234466.1 , XM_024234463.1
Accession Version	XM_024234466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	transcription termination factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG TTAGGTGTCC AGAGCACGGG ACTTTCTGCT TTCTTAAGAC CGGCGTCCGC 
GATGGCCCTA ATAAAGGAAA GAGCTTCTAC GTGTGCCGGG CAGACACGTG CAGCTTCGTG 
CGGCCCACTG ACATTCCTGT TTCCCATTGC TTATTGCATG AGGACTTTGT GGTAGAGCTT 
CAGGGTTTGC TTCTGCCACA GGACAAGAAA GAATACAGAT TGTTCTTCCG ATGCATTAGA 
AGTAAGGCAG AGGGGAAACG CTGGTGTGGA AGTATTCCAT GGCAGGATCC TGATTCCAAA 
GAACATTCTG TACCCAATAA GTCTCAGCAT GCATCTGTGA CATTTCATCA TTCTTCCAAC 
TGGCTGAGAA ATCCATTCAA GGTACTTGAC AAGAATCAAA AACCAGCTCT CCGGAAACAG 
CTCATCAAAG GTGAAGGTGA GGAAAAGACG GCTGATAAGA AGCAAAGAGA AAAGGGAGAT 
CAGCTTTTTG ATCAAAAGAA GGAACAGAAG CTTGAAACGA TGGAGAAAGA TCTCTCATCT 
GGCCTGGTAC CAAAGAAAAA ACAATCTGTA GTTCAAGAGA AGAAGCAAGA AGAGAGAGCA 
GAGATTCAGT GTGAGGCAGA GACTGGAGGC ACACACAAAA GAGACTTTTC TGAAATTAAA 
TCTCAACAGT GCCAAGGTAA TGAGCTTACA AGACCATCTG CATCTTCTCA GGAGAAATCA 
AGTGGTAAGA GTCAAGATGT CCAAAGAGAA TCAGAACCTC TGAGAGAAAA GGTTACCCAG 
CTTTTGCCTC AAAATGTTCA CAGTCCCAAC TCAATAAGCA AGCCCCAGAA AGGGGGACCC 
CTCAACAAGG AGTACACGAA CTGGGAGGCT AAAGAAACAA AGGCAAAGGA TGGCCCTAGC 
ACACAGGCCA CCCAGAAAAG CCTGCCTCGG GGGCATTTCC AAGAGCAGCT GGAGACCCAC 
GGTGTGCCTG CTCCTGGAGG ACCAGCGGCT CAGGCTGCAC CAGCAGCACC AGGGCTTTCC 
CTGGGTGAGG GCCGTGAAGC TGCCACAAGC AGTGATGACG AGGAGGAAGA TGATGTTGTT 
TTTGTTTCCT CTAAGCCTGG GAGCCCCCTA CTCTTTGACT CGACTCTGGA CTTAGAGATG 
AAGGAAAACC TCCAATTCCC TGATCGAAGT GTGCAAAGAA AGGTGTCTCC TGCCTCAGGT 
GTTTCCAAGA AGGTAGAACC CTCAGACCCA GTAGCCCGGC GTGTCTACCT TACGACACAA 
CTGAAACAAA AGAAGAGCAC ACTGGCATCA GTGAACATCC AGGCTCTTCC AGACAAGGGT 
CAAAAGTTGA TCAAACAAAT CCAGGAGCTG GAGGAAGTAC TCAGTGGTCT TGCCCTTTCC 
CCAGAGCAAG GAACTAATGA GAAGGGTAAC AGTCAAGTAC CACAGCAGAG TCACTTCACC 
AAAACTACCA CTGGCCCTCC CCACCTGGTG CCTCCCCAAC CCCTTCCTCA TCGTGGTACC 
CAACCTGTGG GTTCTCTAGA ACTAAAGTCT TCCTGCCAGG TGACTGCTGG AGGATCCAGT 
CAGTGCTATC AAGGTAACAC CTATTTGAAT GCAGGCCATA CAAACCAAGA TCACGTTCAT 
GCAGTGTGGA AAATCACAAG TGAAGCCATC GGTCAACTGC ATCGCTCACT TGAGTCATGT 
CCTGGTGAAA TGGCTGTGGC AGAAGATCCC GCCGGGCTGA AGGTCCCTTT GCTACTACAC 
CAGAAGCAGG CATTGGCTTG GTTACTATGG CGGGAAAGTC AGAAGCCACA AGGAGGAATT 
CTGGCGGATG ATATGGGCTT AGGAAAAACC CTGACAATGA TTGCGCTCAT CCTGACCCAG 
AAGAATCAAG AGAAAAAGAA AGAAAAGGAG AAAAGCACAG CTTTGACATG GCTCTCCAAA 
GATGACTCTT CTGACTTTAC TTCCCATGGA ACATTAATCA TCTGTCCTGC CTCCCTGATC 
CATCATTGGA AAAATGAGGT GGAGAAACGG GTGAACAGCA ACAAACTAAG AGTCTATCTC 
TACCATGGGC CAAACCGGGA TTCACGTGCC AGAGTCCTCT CTACATATGA CATCGTGATC 
ACTACCTATA GCCTCGTGGC CAAGGAGATT CCCACAAGCA AGCAAAAGGC AGAGATCCCA 
GGTGCAAACC TCAATGTGGA GGGCACCTCA ACACCTTTGC TTCGAATAGC CTGGGCTCGA 
ATCATACTGG ATGAAGCTCA CAATGTTAAG AATCCCCGAG TGCAGACGTC CATAGCTGTG 
TGTAAGCTAC AAGCCTGTGC CCGTTGGGCT GTCACTGGAA CCCCCATTCA AAACAACTTA 
TTGGATATGT ATTCACTGCT GAAGTTTCTC CGTTGCTCTC CATTTGATGA GTTCAGTCTG 
TGGAGGAGTC AGGTTGACAA TGGCTCAAAG AAAGGAGGAG AACGGTTAAG TATTTTAACC 
AAGAGCCTTT TGCTGAGGAG AACAAAAGAC CAGCTGGACT CTACTGGCAG ACCTTTGGTG 
ATACTGCCCC AGCGTAAATT TCAGTTGCAC CATTTAAAGC TTTCTGAAGA TGAAGAGACT 
GTTTACAATG TGTTTTTTGC AAGATCAAGG TCAGCTCTGC AATCCTATCT AAAAAGACAT 
GAAAGTAGAG GCAACCAATC TGGAAGAAGC CCTAATAATC CATTCAGTAG AGTGGCACTG 
GAGTTTGGGT CCGAGGAGCC TAGACACTCG GAGGCAGCCG ACTCACCGAG ATCCAGCACC 
GTCCACATAC TGTCCCAGTT GCTGAGACTC CGCCAGTGTT GCTGTCATCT TTCTTTACTA 
AAGTCGGCCC TGGATCCAAT GGAACTGAAG GGTGAAGGTC TTGTCCTTTC CCTGGAAGAA 
CAGCTCAGTG CTTTGACCTT GTCAGAACTC CGTGACTCAG AGCCATCTTC TACTGTTTCC 
CTTAACGGCA CCTTCTTCAA GATGGAGCTT TTTGAAGACA CGCGAGAGAG CACCAAGATT 
TCATCTCTGT TGGCAGAATT GGAGGCAATT CAAAGAAATT CAGCATCCCA AAAGAGTGTC 
ATTGTCTCTC AGTGGACCAA CATGCTGAAA GTTGTAGCAT TGCACCTTAA GAAGCATGGA 
CTGACTTATG CCACCATCGA TGGCTCTGTC AACCCCAAGC AGAGAATGGA CTTGGTAGAG 
GCATTTAACC ACTCCAGAGG CCCTCAGGTA ATGCTAATCT CTCTCTTGGC TGGAGGTGTT 
GGTCTAAACC TGACTGGAGG AAATCACCTC TTTCTTTTGG ACATGCACTG GAATCCATCA 
CTTGAAGATC AAGCTTGTGA CCGAATTTAC CGAGTAGGGC AGCAGAAAGA TGTTGTCATA 
CACAAATTTG TTTGTGAGGG AACAGTAGAA GAAAAGATCT TACAGCTCCA AGAAAAAAAG 
AAAGATTTGG CCAAACAAGT TCTATCAGGG TCTGGAGAAT CTGTCACCAA GCTCACCTTG 
GCTGACCTCA GAGTCCTTTT TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024090234.1
CDS	70..3579
Translation	MEVVRCPEHGTFCFLKTGVRDGPNKGKSFYVCRADTCSFVRPTDIPVSHCLLHEDFVVELQGLLLPQDKKEYRLFFRCIRSKAEGKRWCGSIPWQDPDSKEHSVPNKSQHASVTFHHSSNWLRNPFKVLDKNQKPALRKQLIKGEGEEKTADKKQREKGDQLFDQKKEQKLETMEKDLSSGLVPKKKQSVVQEKKQEERAEIQCEAETGGTHKRDFSEIKSQQCQGNELTRPSASSQEKSSGKSQDVQRESEPLREKVTQLLPQNVHSPNSISKPQKGGPLNKEYTNWEAKETKAKDGPSTQATQKSLPRGHFQEQLETHGVPAPGGPAAQAAPAAPGLSLGEGREAATSSDDEEEDDVVFVSSKPGSPLLFDSTLDLEMKENLQFPDRSVQRKVSPASGVSKKVEPSDPVARRVYLTTQLKQKKSTLASVNIQALPDKGQKLIKQIQELEEVLSGLALSPEQGTNEKGNSQVPQQSHFTKTTTGPPHLVPPQPLPHRGTQPVGSLELKSSCQVTAGGSSQCYQGNTYLNAGHTNQDHVHAVWKITSEAIGQLHRSLESCPGEMAVAEDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGLGKTLTMIALILTQKNQEKKKEKEKSTALTWLSKDDSSDFTSHGTLIICPASLIHHWKNEVEKRVNSNKLRVYLYHGPNRDSRARVLSTYDIVITTYSLVAKEIPTSKQKAEIPGANLNVEGTSTPLLRIAWARIILDEAHNVKNPRVQTSIAVCKLQACARWAVTGTPIQNNLLDMYSLLKFLRCSPFDEFSLWRSQVDNGSKKGGERLSILTKSLLLRRTKDQLDSTGRPLVILPQRKFQLHHLKLSEDEETVYNVFFARSRSALQSYLKRHESRGNQSGRSPNNPFSRVALEFGSEEPRHSEAADSPRSSTVHILSQLLRLRQCCCHLSLLKSALDPMELKGEGLVLSLEEQLSALTLSELRDSEPSSTVSLNGTFFKMELFEDTRESTKISSLLAELEAIQRNSASQKSVIVSQWTNMLKVVALHLKKHGLTYATIDGSVNPKQRMDLVEAFNHSRGPQVMLISLLAGGVGLNLTGGNHLFLLDMHWNPSLEDQACDRIYRVGQQKDVVIHKFVCEGTVEEKILQLQEKKKDLAKQVLSGSGESVTKLTLADLRVLFGI

Target ORF information:

RefSeq Version	XM_024234466.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii transcription termination factor 2 (TTF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024234466.1

ORF Insert Sequence:

ATGGAAGTAG TTAGGTGTCC AGAGCACGGG ACTTTCTGCT TTCTTAAGAC CGGCGTCCGC 
GATGGCCCTA ATAAAGGAAA GAGCTTCTAC GTGTGCCGGG CAGACACGTG CAGCTTCGTG 
CGGCCCACTG ACATTCCTGT TTCCCATTGC TTATTGCATG AGGACTTTGT GGTAGAGCTT 
CAGGGTTTGC TTCTGCCACA GGACAAGAAA GAATACAGAT TGTTCTTCCG ATGCATTAGA 
AGTAAGGCAG AGGGGAAACG CTGGTGTGGA AGTATTCCAT GGCAGGATCC TGATTCCAAA 
GAACATTCTG TACCCAATAA GTCTCAGCAT GCATCTGTGA CATTTCATCA TTCTTCCAAC 
TGGCTGAGAA ATCCATTCAA GGTACTTGAC AAGAATCAAA AACCAGCTCT CCGGAAACAG 
CTCATCAAAG GTGAAGGTGA GGAAAAGACG GCTGATAAGA AGCAAAGAGA AAAGGGAGAT 
CAGCTTTTTG ATCAAAAGAA GGAACAGAAG CTTGAAACGA TGGAGAAAGA TCTCTCATCT 
GGCCTGGTAC CAAAGAAAAA ACAATCTGTA GTTCAAGAGA AGAAGCAAGA AGAGAGAGCA 
GAGATTCAGT GTGAGGCAGA GACTGGAGGC ACACACAAAA GAGACTTTTC TGAAATTAAA 
TCTCAACAGT GCCAAGGTAA TGAGCTTACA AGACCATCTG CATCTTCTCA GGAGAAATCA 
AGTGGTAAGA GTCAAGATGT CCAAAGAGAA TCAGAACCTC TGAGAGAAAA GGTTACCCAG 
CTTTTGCCTC AAAATGTTCA CAGTCCCAAC TCAATAAGCA AGCCCCAGAA AGGGGGACCC 
CTCAACAAGG AGTACACGAA CTGGGAGGCT AAAGAAACAA AGGCAAAGGA TGGCCCTAGC 
ACACAGGCCA CCCAGAAAAG CCTGCCTCGG GGGCATTTCC AAGAGCAGCT GGAGACCCAC 
GGTGTGCCTG CTCCTGGAGG ACCAGCGGCT CAGGCTGCAC CAGCAGCACC AGGGCTTTCC 
CTGGGTGAGG GCCGTGAAGC TGCCACAAGC AGTGATGACG AGGAGGAAGA TGATGTTGTT 
TTTGTTTCCT CTAAGCCTGG GAGCCCCCTA CTCTTTGACT CGACTCTGGA CTTAGAGATG 
AAGGAAAACC TCCAATTCCC TGATCGAAGT GTGCAAAGAA AGGTGTCTCC TGCCTCAGGT 
GTTTCCAAGA AGGTAGAACC CTCAGACCCA GTAGCCCGGC GTGTCTACCT TACGACACAA 
CTGAAACAAA AGAAGAGCAC ACTGGCATCA GTGAACATCC AGGCTCTTCC AGACAAGGGT 
CAAAAGTTGA TCAAACAAAT CCAGGAGCTG GAGGAAGTAC TCAGTGGTCT TGCCCTTTCC 
CCAGAGCAAG GAACTAATGA GAAGGGTAAC AGTCAAGTAC CACAGCAGAG TCACTTCACC 
AAAACTACCA CTGGCCCTCC CCACCTGGTG CCTCCCCAAC CCCTTCCTCA TCGTGGTACC 
CAACCTGTGG GTTCTCTAGA ACTAAAGTCT TCCTGCCAGG TGACTGCTGG AGGATCCAGT 
CAGTGCTATC AAGGTAACAC CTATTTGAAT GCAGGCCATA CAAACCAAGA TCACGTTCAT 
GCAGTGTGGA AAATCACAAG TGAAGCCATC GGTCAACTGC ATCGCTCACT TGAGTCATGT 
CCTGGTGAAA TGGCTGTGGC AGAAGATCCC GCCGGGCTGA AGGTCCCTTT GCTACTACAC 
CAGAAGCAGG CATTGGCTTG GTTACTATGG CGGGAAAGTC AGAAGCCACA AGGAGGAATT 
CTGGCGGATG ATATGGGCTT AGGAAAAACC CTGACAATGA TTGCGCTCAT CCTGACCCAG 
AAGAATCAAG AGAAAAAGAA AGAAAAGGAG AAAAGCACAG CTTTGACATG GCTCTCCAAA 
GATGACTCTT CTGACTTTAC TTCCCATGGA ACATTAATCA TCTGTCCTGC CTCCCTGATC 
CATCATTGGA AAAATGAGGT GGAGAAACGG GTGAACAGCA ACAAACTAAG AGTCTATCTC 
TACCATGGGC CAAACCGGGA TTCACGTGCC AGAGTCCTCT CTACATATGA CATCGTGATC 
ACTACCTATA GCCTCGTGGC CAAGGAGATT CCCACAAGCA AGCAAAAGGC AGAGATCCCA 
GGTGCAAACC TCAATGTGGA GGGCACCTCA ACACCTTTGC TTCGAATAGC CTGGGCTCGA 
ATCATACTGG ATGAAGCTCA CAATGTTAAG AATCCCCGAG TGCAGACGTC CATAGCTGTG 
TGTAAGCTAC AAGCCTGTGC CCGTTGGGCT GTCACTGGAA CCCCCATTCA AAACAACTTA 
TTGGATATGT ATTCACTGCT GAAGTTTCTC CGTTGCTCTC CATTTGATGA GTTCAGTCTG 
TGGAGGAGTC AGGTTGACAA TGGCTCAAAG AAAGGAGGAG AACGGTTAAG TATTTTAACC 
AAGAGCCTTT TGCTGAGGAG AACAAAAGAC CAGCTGGACT CTACTGGCAG ACCTTTGGTG 
ATACTGCCCC AGCGTAAATT TCAGTTGCAC CATTTAAAGC TTTCTGAAGA TGAAGAGACT 
GTTTACAATG TGTTTTTTGC AAGATCAAGG TCAGCTCTGC AATCCTATCT AAAAAGACAT 
GAAAGTAGAG GCAACCAATC TGGAAGAAGC CCTAATAATC CATTCAGTAG AGTGGCACTG 
GAGTTTGGGT CCGAGGAGCC TAGACACTCG GAGGCAGCCG ACTCACCGAG ATCCAGCACC 
GTCCACATAC TGTCCCAGTT GCTGAGACTC CGCCAGTGTT GCTGTCATCT TTCTTTACTA 
AAGTCGGCCC TGGATCCAAT GGAACTGAAG GGTGAAGGTC TTGTCCTTTC CCTGGAAGAA 
CAGCTCAGTG CTTTGACCTT GTCAGAACTC CGTGACTCAG AGCCATCTTC TACTGTTTCC 
CTTAACGGCA CCTTCTTCAA GATGGAGCTT TTTGAAGACA CGCGAGAGAG CACCAAGATT 
TCATCTCTGT TGGCAGAATT GGAGGCAATT CAAAGAAATT CAGCATCCCA AAAGAGTGTC 
ATTGTCTCTC AGTGGACCAA CATGCTGAAA GTTGTAGCAT TGCACCTTAA GAAGCATGGA 
CTGACTTATG CCACCATCGA TGGCTCTGTC AACCCCAAGC AGAGAATGGA CTTGGTAGAG 
GCATTTAACC ACTCCAGAGG CCCTCAGGTA ATGCTAATCT CTCTCTTGGC TGGAGGTGTT 
GGTCTAAACC TGACTGGAGG AAATCACCTC TTTCTTTTGG ACATGCACTG GAATCCATCA 
CTTGAAGATC AAGCTTGTGA CCGAATTTAC CGAGTAGGGC AGCAGAAAGA TGTTGTCATA 
CACAAATTTG TTTGTGAGGG AACAGTAGAA GAAAAGATCT TACAGCTCCA AGAAAAAAAG 
AAAGATTTGG CCAAACAAGT TCTATCAGGG TCTGGAGAAT CTGTCACCAA GCTCACCTTG 
GCTGACCTCA GAGTCCTTTT TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTF2 ( XM_024234463.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024090231.1 Pongo abelii transcription termination factor 2 (TTF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa163440
Clone ID Related Accession (Same CDS sequence)	XM_024234466.1 , XM_024234463.1
Accession Version	XM_024234463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	transcription termination factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG TTAGGTGTCC AGAGCACGGG ACTTTCTGCT TTCTTAAGAC CGGCGTCCGC 
GATGGCCCTA ATAAAGGAAA GAGCTTCTAC GTGTGCCGGG CAGACACGTG CAGCTTCGTG 
CGGCCCACTG ACATTCCTGT TTCCCATTGC TTATTGCATG AGGACTTTGT GGTAGAGCTT 
CAGGGTTTGC TTCTGCCACA GGACAAGAAA GAATACAGAT TGTTCTTCCG ATGCATTAGA 
AGTAAGGCAG AGGGGAAACG CTGGTGTGGA AGTATTCCAT GGCAGGATCC TGATTCCAAA 
GAACATTCTG TACCCAATAA GTCTCAGCAT GCATCTGTGA CATTTCATCA TTCTTCCAAC 
TGGCTGAGAA ATCCATTCAA GGTACTTGAC AAGAATCAAA AACCAGCTCT CCGGAAACAG 
CTCATCAAAG GTGAAGGTGA GGAAAAGACG GCTGATAAGA AGCAAAGAGA AAAGGGAGAT 
CAGCTTTTTG ATCAAAAGAA GGAACAGAAG CTTGAAACGA TGGAGAAAGA TCTCTCATCT 
GGCCTGGTAC CAAAGAAAAA ACAATCTGTA GTTCAAGAGA AGAAGCAAGA AGAGAGAGCA 
GAGATTCAGT GTGAGGCAGA GACTGGAGGC ACACACAAAA GAGACTTTTC TGAAATTAAA 
TCTCAACAGT GCCAAGGTAA TGAGCTTACA AGACCATCTG CATCTTCTCA GGAGAAATCA 
AGTGGTAAGA GTCAAGATGT CCAAAGAGAA TCAGAACCTC TGAGAGAAAA GGTTACCCAG 
CTTTTGCCTC AAAATGTTCA CAGTCCCAAC TCAATAAGCA AGCCCCAGAA AGGGGGACCC 
CTCAACAAGG AGTACACGAA CTGGGAGGCT AAAGAAACAA AGGCAAAGGA TGGCCCTAGC 
ACACAGGCCA CCCAGAAAAG CCTGCCTCGG GGGCATTTCC AAGAGCAGCT GGAGACCCAC 
GGTGTGCCTG CTCCTGGAGG ACCAGCGGCT CAGGCTGCAC CAGCAGCACC AGGGCTTTCC 
CTGGGTGAGG GCCGTGAAGC TGCCACAAGC AGTGATGACG AGGAGGAAGA TGATGTTGTT 
TTTGTTTCCT CTAAGCCTGG GAGCCCCCTA CTCTTTGACT CGACTCTGGA CTTAGAGATG 
AAGGAAAACC TCCAATTCCC TGATCGAAGT GTGCAAAGAA AGGTGTCTCC TGCCTCAGGT 
GTTTCCAAGA AGGTAGAACC CTCAGACCCA GTAGCCCGGC GTGTCTACCT TACGACACAA 
CTGAAACAAA AGAAGAGCAC ACTGGCATCA GTGAACATCC AGGCTCTTCC AGACAAGGGT 
CAAAAGTTGA TCAAACAAAT CCAGGAGCTG GAGGAAGTAC TCAGTGGTCT TGCCCTTTCC 
CCAGAGCAAG GAACTAATGA GAAGGGTAAC AGTCAAGTAC CACAGCAGAG TCACTTCACC 
AAAACTACCA CTGGCCCTCC CCACCTGGTG CCTCCCCAAC CCCTTCCTCA TCGTGGTACC 
CAACCTGTGG GTTCTCTAGA ACTAAAGTCT TCCTGCCAGG TGACTGCTGG AGGATCCAGT 
CAGTGCTATC AAGGTAACAC CTATTTGAAT GCAGGCCATA CAAACCAAGA TCACGTTCAT 
GCAGTGTGGA AAATCACAAG TGAAGCCATC GGTCAACTGC ATCGCTCACT TGAGTCATGT 
CCTGGTGAAA TGGCTGTGGC AGAAGATCCC GCCGGGCTGA AGGTCCCTTT GCTACTACAC 
CAGAAGCAGG CATTGGCTTG GTTACTATGG CGGGAAAGTC AGAAGCCACA AGGAGGAATT 
CTGGCGGATG ATATGGGCTT AGGAAAAACC CTGACAATGA TTGCGCTCAT CCTGACCCAG 
AAGAATCAAG AGAAAAAGAA AGAAAAGGAG AAAAGCACAG CTTTGACATG GCTCTCCAAA 
GATGACTCTT CTGACTTTAC TTCCCATGGA ACATTAATCA TCTGTCCTGC CTCCCTGATC 
CATCATTGGA AAAATGAGGT GGAGAAACGG GTGAACAGCA ACAAACTAAG AGTCTATCTC 
TACCATGGGC CAAACCGGGA TTCACGTGCC AGAGTCCTCT CTACATATGA CATCGTGATC 
ACTACCTATA GCCTCGTGGC CAAGGAGATT CCCACAAGCA AGCAAAAGGC AGAGATCCCA 
GGTGCAAACC TCAATGTGGA GGGCACCTCA ACACCTTTGC TTCGAATAGC CTGGGCTCGA 
ATCATACTGG ATGAAGCTCA CAATGTTAAG AATCCCCGAG TGCAGACGTC CATAGCTGTG 
TGTAAGCTAC AAGCCTGTGC CCGTTGGGCT GTCACTGGAA CCCCCATTCA AAACAACTTA 
TTGGATATGT ATTCACTGCT GAAGTTTCTC CGTTGCTCTC CATTTGATGA GTTCAGTCTG 
TGGAGGAGTC AGGTTGACAA TGGCTCAAAG AAAGGAGGAG AACGGTTAAG TATTTTAACC 
AAGAGCCTTT TGCTGAGGAG AACAAAAGAC CAGCTGGACT CTACTGGCAG ACCTTTGGTG 
ATACTGCCCC AGCGTAAATT TCAGTTGCAC CATTTAAAGC TTTCTGAAGA TGAAGAGACT 
GTTTACAATG TGTTTTTTGC AAGATCAAGG TCAGCTCTGC AATCCTATCT AAAAAGACAT 
GAAAGTAGAG GCAACCAATC TGGAAGAAGC CCTAATAATC CATTCAGTAG AGTGGCACTG 
GAGTTTGGGT CCGAGGAGCC TAGACACTCG GAGGCAGCCG ACTCACCGAG ATCCAGCACC 
GTCCACATAC TGTCCCAGTT GCTGAGACTC CGCCAGTGTT GCTGTCATCT TTCTTTACTA 
AAGTCGGCCC TGGATCCAAT GGAACTGAAG GGTGAAGGTC TTGTCCTTTC CCTGGAAGAA 
CAGCTCAGTG CTTTGACCTT GTCAGAACTC CGTGACTCAG AGCCATCTTC TACTGTTTCC 
CTTAACGGCA CCTTCTTCAA GATGGAGCTT TTTGAAGACA CGCGAGAGAG CACCAAGATT 
TCATCTCTGT TGGCAGAATT GGAGGCAATT CAAAGAAATT CAGCATCCCA AAAGAGTGTC 
ATTGTCTCTC AGTGGACCAA CATGCTGAAA GTTGTAGCAT TGCACCTTAA GAAGCATGGA 
CTGACTTATG CCACCATCGA TGGCTCTGTC AACCCCAAGC AGAGAATGGA CTTGGTAGAG 
GCATTTAACC ACTCCAGAGG CCCTCAGGTA ATGCTAATCT CTCTCTTGGC TGGAGGTGTT 
GGTCTAAACC TGACTGGAGG AAATCACCTC TTTCTTTTGG ACATGCACTG GAATCCATCA 
CTTGAAGATC AAGCTTGTGA CCGAATTTAC CGAGTAGGGC AGCAGAAAGA TGTTGTCATA 
CACAAATTTG TTTGTGAGGG AACAGTAGAA GAAAAGATCT TACAGCTCCA AGAAAAAAAG 
AAAGATTTGG CCAAACAAGT TCTATCAGGG TCTGGAGAAT CTGTCACCAA GCTCACCTTG 
GCTGACCTCA GAGTCCTTTT TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024090231.1
CDS	70..3579
Translation	MEVVRCPEHGTFCFLKTGVRDGPNKGKSFYVCRADTCSFVRPTDIPVSHCLLHEDFVVELQGLLLPQDKKEYRLFFRCIRSKAEGKRWCGSIPWQDPDSKEHSVPNKSQHASVTFHHSSNWLRNPFKVLDKNQKPALRKQLIKGEGEEKTADKKQREKGDQLFDQKKEQKLETMEKDLSSGLVPKKKQSVVQEKKQEERAEIQCEAETGGTHKRDFSEIKSQQCQGNELTRPSASSQEKSSGKSQDVQRESEPLREKVTQLLPQNVHSPNSISKPQKGGPLNKEYTNWEAKETKAKDGPSTQATQKSLPRGHFQEQLETHGVPAPGGPAAQAAPAAPGLSLGEGREAATSSDDEEEDDVVFVSSKPGSPLLFDSTLDLEMKENLQFPDRSVQRKVSPASGVSKKVEPSDPVARRVYLTTQLKQKKSTLASVNIQALPDKGQKLIKQIQELEEVLSGLALSPEQGTNEKGNSQVPQQSHFTKTTTGPPHLVPPQPLPHRGTQPVGSLELKSSCQVTAGGSSQCYQGNTYLNAGHTNQDHVHAVWKITSEAIGQLHRSLESCPGEMAVAEDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGLGKTLTMIALILTQKNQEKKKEKEKSTALTWLSKDDSSDFTSHGTLIICPASLIHHWKNEVEKRVNSNKLRVYLYHGPNRDSRARVLSTYDIVITTYSLVAKEIPTSKQKAEIPGANLNVEGTSTPLLRIAWARIILDEAHNVKNPRVQTSIAVCKLQACARWAVTGTPIQNNLLDMYSLLKFLRCSPFDEFSLWRSQVDNGSKKGGERLSILTKSLLLRRTKDQLDSTGRPLVILPQRKFQLHHLKLSEDEETVYNVFFARSRSALQSYLKRHESRGNQSGRSPNNPFSRVALEFGSEEPRHSEAADSPRSSTVHILSQLLRLRQCCCHLSLLKSALDPMELKGEGLVLSLEEQLSALTLSELRDSEPSSTVSLNGTFFKMELFEDTRESTKISSLLAELEAIQRNSASQKSVIVSQWTNMLKVVALHLKKHGLTYATIDGSVNPKQRMDLVEAFNHSRGPQVMLISLLAGGVGLNLTGGNHLFLLDMHWNPSLEDQACDRIYRVGQQKDVVIHKFVCEGTVEEKILQLQEKKKDLAKQVLSGSGESVTKLTLADLRVLFGI

Target ORF information:

RefSeq Version	XM_024234463.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii transcription termination factor 2 (TTF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024234463.1

ORF Insert Sequence:

ATGGAAGTAG TTAGGTGTCC AGAGCACGGG ACTTTCTGCT TTCTTAAGAC CGGCGTCCGC 
GATGGCCCTA ATAAAGGAAA GAGCTTCTAC GTGTGCCGGG CAGACACGTG CAGCTTCGTG 
CGGCCCACTG ACATTCCTGT TTCCCATTGC TTATTGCATG AGGACTTTGT GGTAGAGCTT 
CAGGGTTTGC TTCTGCCACA GGACAAGAAA GAATACAGAT TGTTCTTCCG ATGCATTAGA 
AGTAAGGCAG AGGGGAAACG CTGGTGTGGA AGTATTCCAT GGCAGGATCC TGATTCCAAA 
GAACATTCTG TACCCAATAA GTCTCAGCAT GCATCTGTGA CATTTCATCA TTCTTCCAAC 
TGGCTGAGAA ATCCATTCAA GGTACTTGAC AAGAATCAAA AACCAGCTCT CCGGAAACAG 
CTCATCAAAG GTGAAGGTGA GGAAAAGACG GCTGATAAGA AGCAAAGAGA AAAGGGAGAT 
CAGCTTTTTG ATCAAAAGAA GGAACAGAAG CTTGAAACGA TGGAGAAAGA TCTCTCATCT 
GGCCTGGTAC CAAAGAAAAA ACAATCTGTA GTTCAAGAGA AGAAGCAAGA AGAGAGAGCA 
GAGATTCAGT GTGAGGCAGA GACTGGAGGC ACACACAAAA GAGACTTTTC TGAAATTAAA 
TCTCAACAGT GCCAAGGTAA TGAGCTTACA AGACCATCTG CATCTTCTCA GGAGAAATCA 
AGTGGTAAGA GTCAAGATGT CCAAAGAGAA TCAGAACCTC TGAGAGAAAA GGTTACCCAG 
CTTTTGCCTC AAAATGTTCA CAGTCCCAAC TCAATAAGCA AGCCCCAGAA AGGGGGACCC 
CTCAACAAGG AGTACACGAA CTGGGAGGCT AAAGAAACAA AGGCAAAGGA TGGCCCTAGC 
ACACAGGCCA CCCAGAAAAG CCTGCCTCGG GGGCATTTCC AAGAGCAGCT GGAGACCCAC 
GGTGTGCCTG CTCCTGGAGG ACCAGCGGCT CAGGCTGCAC CAGCAGCACC AGGGCTTTCC 
CTGGGTGAGG GCCGTGAAGC TGCCACAAGC AGTGATGACG AGGAGGAAGA TGATGTTGTT 
TTTGTTTCCT CTAAGCCTGG GAGCCCCCTA CTCTTTGACT CGACTCTGGA CTTAGAGATG 
AAGGAAAACC TCCAATTCCC TGATCGAAGT GTGCAAAGAA AGGTGTCTCC TGCCTCAGGT 
GTTTCCAAGA AGGTAGAACC CTCAGACCCA GTAGCCCGGC GTGTCTACCT TACGACACAA 
CTGAAACAAA AGAAGAGCAC ACTGGCATCA GTGAACATCC AGGCTCTTCC AGACAAGGGT 
CAAAAGTTGA TCAAACAAAT CCAGGAGCTG GAGGAAGTAC TCAGTGGTCT TGCCCTTTCC 
CCAGAGCAAG GAACTAATGA GAAGGGTAAC AGTCAAGTAC CACAGCAGAG TCACTTCACC 
AAAACTACCA CTGGCCCTCC CCACCTGGTG CCTCCCCAAC CCCTTCCTCA TCGTGGTACC 
CAACCTGTGG GTTCTCTAGA ACTAAAGTCT TCCTGCCAGG TGACTGCTGG AGGATCCAGT 
CAGTGCTATC AAGGTAACAC CTATTTGAAT GCAGGCCATA CAAACCAAGA TCACGTTCAT 
GCAGTGTGGA AAATCACAAG TGAAGCCATC GGTCAACTGC ATCGCTCACT TGAGTCATGT 
CCTGGTGAAA TGGCTGTGGC AGAAGATCCC GCCGGGCTGA AGGTCCCTTT GCTACTACAC 
CAGAAGCAGG CATTGGCTTG GTTACTATGG CGGGAAAGTC AGAAGCCACA AGGAGGAATT 
CTGGCGGATG ATATGGGCTT AGGAAAAACC CTGACAATGA TTGCGCTCAT CCTGACCCAG 
AAGAATCAAG AGAAAAAGAA AGAAAAGGAG AAAAGCACAG CTTTGACATG GCTCTCCAAA 
GATGACTCTT CTGACTTTAC TTCCCATGGA ACATTAATCA TCTGTCCTGC CTCCCTGATC 
CATCATTGGA AAAATGAGGT GGAGAAACGG GTGAACAGCA ACAAACTAAG AGTCTATCTC 
TACCATGGGC CAAACCGGGA TTCACGTGCC AGAGTCCTCT CTACATATGA CATCGTGATC 
ACTACCTATA GCCTCGTGGC CAAGGAGATT CCCACAAGCA AGCAAAAGGC AGAGATCCCA 
GGTGCAAACC TCAATGTGGA GGGCACCTCA ACACCTTTGC TTCGAATAGC CTGGGCTCGA 
ATCATACTGG ATGAAGCTCA CAATGTTAAG AATCCCCGAG TGCAGACGTC CATAGCTGTG 
TGTAAGCTAC AAGCCTGTGC CCGTTGGGCT GTCACTGGAA CCCCCATTCA AAACAACTTA 
TTGGATATGT ATTCACTGCT GAAGTTTCTC CGTTGCTCTC CATTTGATGA GTTCAGTCTG 
TGGAGGAGTC AGGTTGACAA TGGCTCAAAG AAAGGAGGAG AACGGTTAAG TATTTTAACC 
AAGAGCCTTT TGCTGAGGAG AACAAAAGAC CAGCTGGACT CTACTGGCAG ACCTTTGGTG 
ATACTGCCCC AGCGTAAATT TCAGTTGCAC CATTTAAAGC TTTCTGAAGA TGAAGAGACT 
GTTTACAATG TGTTTTTTGC AAGATCAAGG TCAGCTCTGC AATCCTATCT AAAAAGACAT 
GAAAGTAGAG GCAACCAATC TGGAAGAAGC CCTAATAATC CATTCAGTAG AGTGGCACTG 
GAGTTTGGGT CCGAGGAGCC TAGACACTCG GAGGCAGCCG ACTCACCGAG ATCCAGCACC 
GTCCACATAC TGTCCCAGTT GCTGAGACTC CGCCAGTGTT GCTGTCATCT TTCTTTACTA 
AAGTCGGCCC TGGATCCAAT GGAACTGAAG GGTGAAGGTC TTGTCCTTTC CCTGGAAGAA 
CAGCTCAGTG CTTTGACCTT GTCAGAACTC CGTGACTCAG AGCCATCTTC TACTGTTTCC 
CTTAACGGCA CCTTCTTCAA GATGGAGCTT TTTGAAGACA CGCGAGAGAG CACCAAGATT 
TCATCTCTGT TGGCAGAATT GGAGGCAATT CAAAGAAATT CAGCATCCCA AAAGAGTGTC 
ATTGTCTCTC AGTGGACCAA CATGCTGAAA GTTGTAGCAT TGCACCTTAA GAAGCATGGA 
CTGACTTATG CCACCATCGA TGGCTCTGTC AACCCCAAGC AGAGAATGGA CTTGGTAGAG 
GCATTTAACC ACTCCAGAGG CCCTCAGGTA ATGCTAATCT CTCTCTTGGC TGGAGGTGTT 
GGTCTAAACC TGACTGGAGG AAATCACCTC TTTCTTTTGG ACATGCACTG GAATCCATCA 
CTTGAAGATC AAGCTTGTGA CCGAATTTAC CGAGTAGGGC AGCAGAAAGA TGTTGTCATA 
CACAAATTTG TTTGTGAGGG AACAGTAGAA GAAAAGATCT TACAGCTCCA AGAAAAAAAG 
AAAGATTTGG CCAAACAAGT TCTATCAGGG TCTGGAGAAT CTGTCACCAA GCTCACCTTG 
GCTGACCTCA GAGTCCTTTT TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTF2 ( XM_024234473.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024090241.1 Pongo abelii transcription termination factor 2 (TTF2), transcript variant X3, mRNA.

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CloneID	OSa163441
Clone ID Related Accession (Same CDS sequence)	XM_024234473.1
Accession Version	XM_024234473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	transcription termination factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036903.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG TTAGGTGTCC AGAGCACGGG ACTTTCTGCT TTCTTAAGAC CGGCGTCCGC 
GATGGCCCTA ATAAAGGAAA GAGCTTCTAC GTGTGCCGGG CAGACACGTG CAGCTTCGTG 
CGGCCCACTG ACATTCCTGT TTCCCATTGC TTATTGCATG AGGACTTTGT GGTAGAGCTT 
CAGGGTTTGC TTCTGCCACA GGACAAGAAA GAATACAGAT TGTTCTTCCG ATGCATTAGA 
AGTAAGGCAG AGGGGAAACG CTGGTGTGGA AGTATTCCAT GGCAGGATCC TGATTCCAAA 
GAACATTCTG TACCCAATAA GTCTCAGCAT GCATCTGTGA CATTTCATCA TTCTTCCAAC 
TGGCTGAGAA ATCCATTCAA GGTACTTGAC AAGAATCAAA AACCAGCTCT CCGGAAACAG 
CTCATCAAAG GTGAAGGTGA GGAAAAGACG GCTGATAAGA AGCAAAGAGA AAAGGGAGAT 
CAGCTTTTTG ATCAAAAGAA GGAACAGAAG CTTGAAACGA TGGAGAAAGA TCTCTCATCT 
GGCCTGGTAC CAAAGAAAAA ACAATCTGTA GTTCAAGAGA AGAAGCAAGA AGAGAGAGCA 
GAGATTCAGT GTGAGGCAGA GACTGGAGGC ACACACAAAA GAGACTTTTC TGAAATTAAA 
TCTCAACAGT GCCAAGGTAA TGAGCTTACA AGACCATCTG CATCTTCTCA GGAGAAATCA 
AGTGGTAAGA GTCAAGATGT CCAAAGAGAA TCAGAACCTC TGAGAGAAAA GGTTACCCAG 
CTTTTGCCTC AAAATGTTCA CAGTCCCAAC TCAATAAGCA AGCCCCAGAA AGGGGGACCC 
CTCAACAAGG AGTACACGAA CTGGGAGGCT AAAGAAACAA AGGCAAAGGA TGGCCCTAGC 
ACACAGGCCA CCCAGAAAAG CCTGCCTCGG GGGCATTTCC AAGAGCAGCT GGAGACCCAC 
GGTGTGCCTG CTCCTGGAGG ACCAGCGGCT CAGGCTGCAC CAGCAGCACC AGGGCTTTCC 
CTGGGTGAGG GCCGTGAAGC TGCCACAAGC AGTGATGACG AGGAGGAAGA TGATGTTGTT 
TTTGTTTCCT CTAAGCCTGG GAGCCCCCTA CTCTTTGACT CGACTCTGGA CTTAGAGATG 
AAGGAAAACC TCCAATTCCC TGATCGAAGT GTGCAAAGAA AGGTGTCTCC TGCCTCAGGT 
GTTTCCAAGA AGGTAGAACC CTCAGACCCA GTAGCCCGGC GTGTCTACCT TACGACACAA 
CTGAAACAAA AGAAGAGCAC ACTGGCATCA GTGAACATCC AGGCTCTTCC AGACAAGGGT 
CAAAAGTTGA TCAAACAAAT CCAGGAGCTG GAGGAAGTAC TCAGTGGTCT TGCCCTTTCC 
CCAGAGCAAG GAACTAATGA GAAGGGTAAC AGTCAAGTAC CACAGCAGAG TCACTTCACC 
AAAACTACCA CTGGCCCTCC CCACCTGGTG CCTCCCCAAC CCCTTCCTCA TCGTGGTACC 
CAACCTGTGG GTTCTCTAGA ACTAAAGTCT TCCTGCCAGG TGACTGCTGG AGGATCCAGT 
CAGTGCTATC AAGGCCATAC AAACCAAGAT CACGTTCATG CAGTGTGGAA AATCACAAGT 
GAAGCCATCG GTCAACTGCA TCGCTCACTT GAGTCATGTC CTGGTGAAAT GGCTGTGGCA 
GAAGATCCCG CCGGGCTGAA GGTCCCTTTG CTACTACACC AGAAGCAGGC ATTGGCTTGG 
TTACTATGGC GGGAAAGTCA GAAGCCACAA GGAGGAATTC TGGCGGATGA TATGGGCTTA 
GGAAAAACCC TGACAATGAT TGCGCTCATC CTGACCCAGA AGAATCAAGA GAAAAAGAAA 
GAAAAGGAGA AAAGCACAGC TTTGACATGG CTCTCCAAAG ATGACTCTTC TGACTTTACT 
TCCCATGGAA CATTAATCAT CTGTCCTGCC TCCCTGATCC ATCATTGGAA AAATGAGGTG 
GAGAAACGGG TGAACAGCAA CAAACTAAGA GTCTATCTCT ACCATGGGCC AAACCGGGAT 
TCACGTGCCA GAGTCCTCTC TACATATGAC ATCGTGATCA CTACCTATAG CCTCGTGGCC 
AAGGAGATTC CCACAAGCAA GCAAAAGGCA GAGATCCCAG GTGCAAACCT CAATGTGGAG 
GGCACCTCAA CACCTTTGCT TCGAATAGCC TGGGCTCGAA TCATACTGGA TGAAGCTCAC 
AATGTTAAGA ATCCCCGAGT GCAGACGTCC ATAGCTGTGT GTAAGCTACA AGCCTGTGCC 
CGTTGGGCTG TCACTGGAAC CCCCATTCAA AACAACTTAT TGGATATGTA TTCACTGCTG 
AAGTTTCTCC GTTGCTCTCC ATTTGATGAG TTCAGTCTGT GGAGGAGTCA GGTTGACAAT 
GGCTCAAAGA AAGGAGGAGA ACGGTTAAGT ATTTTAACCA AGAGCCTTTT GCTGAGGAGA 
ACAAAAGACC AGCTGGACTC TACTGGCAGA CCTTTGGTGA TACTGCCCCA GCGTAAATTT 
CAGTTGCACC ATTTAAAGCT TTCTGAAGAT GAAGAGACTG TTTACAATGT GTTTTTTGCA 
AGATCAAGGT CAGCTCTGCA ATCCTATCTA AAAAGACATG AAAGTAGAGG CAACCAATCT 
GGAAGAAGCC CTAATAATCC ATTCAGTAGA GTGGCACTGG AGTTTGGGTC CGAGGAGCCT 
AGACACTCGG AGGCAGCCGA CTCACCGAGA TCCAGCACCG TCCACATACT GTCCCAGTTG 
CTGAGACTCC GCCAGTGTTG CTGTCATCTT TCTTTACTAA AGTCGGCCCT GGATCCAATG 
GAACTGAAGG GTGAAGGTCT TGTCCTTTCC CTGGAAGAAC AGCTCAGTGC TTTGACCTTG 
TCAGAACTCC GTGACTCAGA GCCATCTTCT ACTGTTTCCC TTAACGGCAC CTTCTTCAAG 
ATGGAGCTTT TTGAAGACAC GCGAGAGAGC ACCAAGATTT CATCTCTGTT GGCAGAATTG 
GAGGCAATTC AAAGAAATTC AGCATCCCAA AAGAGTGTCA TTGTCTCTCA GTGGACCAAC 
ATGCTGAAAG TTGTAGCATT GCACCTTAAG AAGCATGGAC TGACTTATGC CACCATCGAT 
GGCTCTGTCA ACCCCAAGCA GAGAATGGAC TTGGTAGAGG CATTTAACCA CTCCAGAGGC 
CCTCAGGTAA TGCTAATCTC TCTCTTGGCT GGAGGTGTTG GTCTAAACCT GACTGGAGGA 
AATCACCTCT TTCTTTTGGA CATGCACTGG AATCCATCAC TTGAAGATCA AGCTTGTGAC 
CGAATTTACC GAGTAGGGCA GCAGAAAGAT GTTGTCATAC ACAAATTTGT TTGTGAGGGA 
ACAGTAGAAG AAAAGATCTT ACAGCTCCAA GAAAAAAAGA AAGATTTGGC CAAACAAGTT 
CTATCAGGGT CTGGAGAATC TGTCACCAAG CTCACCTTGG CTGACCTCAG AGTCCTTTTT 
GGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024090241.1
CDS	70..3558
Translation	MEVVRCPEHGTFCFLKTGVRDGPNKGKSFYVCRADTCSFVRPTDIPVSHCLLHEDFVVELQGLLLPQDKKEYRLFFRCIRSKAEGKRWCGSIPWQDPDSKEHSVPNKSQHASVTFHHSSNWLRNPFKVLDKNQKPALRKQLIKGEGEEKTADKKQREKGDQLFDQKKEQKLETMEKDLSSGLVPKKKQSVVQEKKQEERAEIQCEAETGGTHKRDFSEIKSQQCQGNELTRPSASSQEKSSGKSQDVQRESEPLREKVTQLLPQNVHSPNSISKPQKGGPLNKEYTNWEAKETKAKDGPSTQATQKSLPRGHFQEQLETHGVPAPGGPAAQAAPAAPGLSLGEGREAATSSDDEEEDDVVFVSSKPGSPLLFDSTLDLEMKENLQFPDRSVQRKVSPASGVSKKVEPSDPVARRVYLTTQLKQKKSTLASVNIQALPDKGQKLIKQIQELEEVLSGLALSPEQGTNEKGNSQVPQQSHFTKTTTGPPHLVPPQPLPHRGTQPVGSLELKSSCQVTAGGSSQCYQGHTNQDHVHAVWKITSEAIGQLHRSLESCPGEMAVAEDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGLGKTLTMIALILTQKNQEKKKEKEKSTALTWLSKDDSSDFTSHGTLIICPASLIHHWKNEVEKRVNSNKLRVYLYHGPNRDSRARVLSTYDIVITTYSLVAKEIPTSKQKAEIPGANLNVEGTSTPLLRIAWARIILDEAHNVKNPRVQTSIAVCKLQACARWAVTGTPIQNNLLDMYSLLKFLRCSPFDEFSLWRSQVDNGSKKGGERLSILTKSLLLRRTKDQLDSTGRPLVILPQRKFQLHHLKLSEDEETVYNVFFARSRSALQSYLKRHESRGNQSGRSPNNPFSRVALEFGSEEPRHSEAADSPRSSTVHILSQLLRLRQCCCHLSLLKSALDPMELKGEGLVLSLEEQLSALTLSELRDSEPSSTVSLNGTFFKMELFEDTRESTKISSLLAELEAIQRNSASQKSVIVSQWTNMLKVVALHLKKHGLTYATIDGSVNPKQRMDLVEAFNHSRGPQVMLISLLAGGVGLNLTGGNHLFLLDMHWNPSLEDQACDRIYRVGQQKDVVIHKFVCEGTVEEKILQLQEKKKDLAKQVLSGSGESVTKLTLADLRVLFGI

Target ORF information:

RefSeq Version	XM_024234473.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii transcription termination factor 2 (TTF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024234473.1

ORF Insert Sequence:

ATGGAAGTAG TTAGGTGTCC AGAGCACGGG ACTTTCTGCT TTCTTAAGAC CGGCGTCCGC 
GATGGCCCTA ATAAAGGAAA GAGCTTCTAC GTGTGCCGGG CAGACACGTG CAGCTTCGTG 
CGGCCCACTG ACATTCCTGT TTCCCATTGC TTATTGCATG AGGACTTTGT GGTAGAGCTT 
CAGGGTTTGC TTCTGCCACA GGACAAGAAA GAATACAGAT TGTTCTTCCG ATGCATTAGA 
AGTAAGGCAG AGGGGAAACG CTGGTGTGGA AGTATTCCAT GGCAGGATCC TGATTCCAAA 
GAACATTCTG TACCCAATAA GTCTCAGCAT GCATCTGTGA CATTTCATCA TTCTTCCAAC 
TGGCTGAGAA ATCCATTCAA GGTACTTGAC AAGAATCAAA AACCAGCTCT CCGGAAACAG 
CTCATCAAAG GTGAAGGTGA GGAAAAGACG GCTGATAAGA AGCAAAGAGA AAAGGGAGAT 
CAGCTTTTTG ATCAAAAGAA GGAACAGAAG CTTGAAACGA TGGAGAAAGA TCTCTCATCT 
GGCCTGGTAC CAAAGAAAAA ACAATCTGTA GTTCAAGAGA AGAAGCAAGA AGAGAGAGCA 
GAGATTCAGT GTGAGGCAGA GACTGGAGGC ACACACAAAA GAGACTTTTC TGAAATTAAA 
TCTCAACAGT GCCAAGGTAA TGAGCTTACA AGACCATCTG CATCTTCTCA GGAGAAATCA 
AGTGGTAAGA GTCAAGATGT CCAAAGAGAA TCAGAACCTC TGAGAGAAAA GGTTACCCAG 
CTTTTGCCTC AAAATGTTCA CAGTCCCAAC TCAATAAGCA AGCCCCAGAA AGGGGGACCC 
CTCAACAAGG AGTACACGAA CTGGGAGGCT AAAGAAACAA AGGCAAAGGA TGGCCCTAGC 
ACACAGGCCA CCCAGAAAAG CCTGCCTCGG GGGCATTTCC AAGAGCAGCT GGAGACCCAC 
GGTGTGCCTG CTCCTGGAGG ACCAGCGGCT CAGGCTGCAC CAGCAGCACC AGGGCTTTCC 
CTGGGTGAGG GCCGTGAAGC TGCCACAAGC AGTGATGACG AGGAGGAAGA TGATGTTGTT 
TTTGTTTCCT CTAAGCCTGG GAGCCCCCTA CTCTTTGACT CGACTCTGGA CTTAGAGATG 
AAGGAAAACC TCCAATTCCC TGATCGAAGT GTGCAAAGAA AGGTGTCTCC TGCCTCAGGT 
GTTTCCAAGA AGGTAGAACC CTCAGACCCA GTAGCCCGGC GTGTCTACCT TACGACACAA 
CTGAAACAAA AGAAGAGCAC ACTGGCATCA GTGAACATCC AGGCTCTTCC AGACAAGGGT 
CAAAAGTTGA TCAAACAAAT CCAGGAGCTG GAGGAAGTAC TCAGTGGTCT TGCCCTTTCC 
CCAGAGCAAG GAACTAATGA GAAGGGTAAC AGTCAAGTAC CACAGCAGAG TCACTTCACC 
AAAACTACCA CTGGCCCTCC CCACCTGGTG CCTCCCCAAC CCCTTCCTCA TCGTGGTACC 
CAACCTGTGG GTTCTCTAGA ACTAAAGTCT TCCTGCCAGG TGACTGCTGG AGGATCCAGT 
CAGTGCTATC AAGGCCATAC AAACCAAGAT CACGTTCATG CAGTGTGGAA AATCACAAGT 
GAAGCCATCG GTCAACTGCA TCGCTCACTT GAGTCATGTC CTGGTGAAAT GGCTGTGGCA 
GAAGATCCCG CCGGGCTGAA GGTCCCTTTG CTACTACACC AGAAGCAGGC ATTGGCTTGG 
TTACTATGGC GGGAAAGTCA GAAGCCACAA GGAGGAATTC TGGCGGATGA TATGGGCTTA 
GGAAAAACCC TGACAATGAT TGCGCTCATC CTGACCCAGA AGAATCAAGA GAAAAAGAAA 
GAAAAGGAGA AAAGCACAGC TTTGACATGG CTCTCCAAAG ATGACTCTTC TGACTTTACT 
TCCCATGGAA CATTAATCAT CTGTCCTGCC TCCCTGATCC ATCATTGGAA AAATGAGGTG 
GAGAAACGGG TGAACAGCAA CAAACTAAGA GTCTATCTCT ACCATGGGCC AAACCGGGAT 
TCACGTGCCA GAGTCCTCTC TACATATGAC ATCGTGATCA CTACCTATAG CCTCGTGGCC 
AAGGAGATTC CCACAAGCAA GCAAAAGGCA GAGATCCCAG GTGCAAACCT CAATGTGGAG 
GGCACCTCAA CACCTTTGCT TCGAATAGCC TGGGCTCGAA TCATACTGGA TGAAGCTCAC 
AATGTTAAGA ATCCCCGAGT GCAGACGTCC ATAGCTGTGT GTAAGCTACA AGCCTGTGCC 
CGTTGGGCTG TCACTGGAAC CCCCATTCAA AACAACTTAT TGGATATGTA TTCACTGCTG 
AAGTTTCTCC GTTGCTCTCC ATTTGATGAG TTCAGTCTGT GGAGGAGTCA GGTTGACAAT 
GGCTCAAAGA AAGGAGGAGA ACGGTTAAGT ATTTTAACCA AGAGCCTTTT GCTGAGGAGA 
ACAAAAGACC AGCTGGACTC TACTGGCAGA CCTTTGGTGA TACTGCCCCA GCGTAAATTT 
CAGTTGCACC ATTTAAAGCT TTCTGAAGAT GAAGAGACTG TTTACAATGT GTTTTTTGCA 
AGATCAAGGT CAGCTCTGCA ATCCTATCTA AAAAGACATG AAAGTAGAGG CAACCAATCT 
GGAAGAAGCC CTAATAATCC ATTCAGTAGA GTGGCACTGG AGTTTGGGTC CGAGGAGCCT 
AGACACTCGG AGGCAGCCGA CTCACCGAGA TCCAGCACCG TCCACATACT GTCCCAGTTG 
CTGAGACTCC GCCAGTGTTG CTGTCATCTT TCTTTACTAA AGTCGGCCCT GGATCCAATG 
GAACTGAAGG GTGAAGGTCT TGTCCTTTCC CTGGAAGAAC AGCTCAGTGC TTTGACCTTG 
TCAGAACTCC GTGACTCAGA GCCATCTTCT ACTGTTTCCC TTAACGGCAC CTTCTTCAAG 
ATGGAGCTTT TTGAAGACAC GCGAGAGAGC ACCAAGATTT CATCTCTGTT GGCAGAATTG 
GAGGCAATTC AAAGAAATTC AGCATCCCAA AAGAGTGTCA TTGTCTCTCA GTGGACCAAC 
ATGCTGAAAG TTGTAGCATT GCACCTTAAG AAGCATGGAC TGACTTATGC CACCATCGAT 
GGCTCTGTCA ACCCCAAGCA GAGAATGGAC TTGGTAGAGG CATTTAACCA CTCCAGAGGC 
CCTCAGGTAA TGCTAATCTC TCTCTTGGCT GGAGGTGTTG GTCTAAACCT GACTGGAGGA 
AATCACCTCT TTCTTTTGGA CATGCACTGG AATCCATCAC TTGAAGATCA AGCTTGTGAC 
CGAATTTACC GAGTAGGGCA GCAGAAAGAT GTTGTCATAC ACAAATTTGT TTGTGAGGGA 
ACAGTAGAAG AAAAGATCTT ACAGCTCCAA GAAAAAAAGA AAGATTTGGC CAAACAAGTT 
CTATCAGGGT CTGGAGAATC TGTCACCAAG CTCACCTTGG CTGACCTCAG AGTCCTTTTT 
GGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTF2 ( XM_002810380.3 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_002810426.2 Pongo abelii transcription termination factor, RNA polymerase II (TTF2), mRNA.

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CloneID	OSa01319
Clone ID Related Accession (Same CDS sequence)	XM_002810380.3
Accession Version	XM_002810380.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	transcription termination factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002875094.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 23, 2014 this sequence version replaced XM_002810380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAAGTAG TTAGGTGTCC AGAGCACGGG ACTTTCTGCT TTCTTAAGAC CGGCGTCCGC 
GATGGCCCTA ATAAAGGAAA GAGCTTCTAC GTGTGCCGGG CAGACACGTG CAGCTTCGTG 
CGGCCCACTG ACATTCCTGT TTCCCATTGC TTATTGCATG AGGACTTTGT GGTAGAGCTT 
CAGGGTTTGC TTCTGCCACA GGACAAGAAA GAATACAGAT TGTTCTTCCG ATGCATTAGA 
AGTAAGGCAG AGGGGAAACG CTGGTGTGGA AGTATTCCAT GGCAGGATCC TGATTCCAAA 
GAACATTCTG TACCCAATAA GTCTCAGCAT GCATCTGTGA CATTTCATCA TTCTTCCAAC 
TGGCTGAGAA ATCCATTCAA GGTACTTGAC AAGAATCAAA AACCAGCTCT CTGGAAACAG 
CTCATCAAAG GTGAAGGTGA GGAAAAGACG GCTGATAAGA AGCAAAGAGA AAAGGGAGAT 
CAGCTTTTTG ATCAAAAGAA GGAACAGAAG CTTGAAACGA TGGAGAAAGA TCTCTCATCT 
GGCCTGGTAC CAAAGAAAAA ACAATCTGTA GTTCAAGAGA AGAAGCAAGA AGAGAGAGCA 
GAGATTCAGT GTGAGGCAGA GACTGGAGGC ACACACAAAA GAGACTTTTC TGAAATTAAA 
TCTCAACAGT GCCAAGGTAA TGAGCTTACA AGACCATCTG CATCTTCTCA GGAGAAATCA 
AGTGGTAAGA GTCAAGATGT CCAAAGAGAA TCAGAACCTC TGAGAGAAAA GGTTACCCAG 
CTTTTGCCTC AAAATGTTCA CAGTCCCAAC TCAATAAGCA AGCCCCAGAA AGGGGGACCC 
CTCAACAAGG AGTACACGAA CTGGGAGGCT AAAGAAACAA AGGCAAAGGA TGGCCCTAGC 
ACACAGGCCA CCCAGAAAAG CCTGCCTCGG GGGCATTTCC AAGAGCAGCC GGAGACCCAC 
GGTGTGCCTG CTCCTGGAGG ACCAGCGGCT CAGGCTGCAC CAGCAGCACC AGGGCTTTCC 
CTGGGTGAGG GCCGTGAAGC TGCCACAAGC AGTGATGACG AGGAGGAAGA TGATGTTGTT 
TTTGTTTCCT CTAAGCCTGG GAGCCCCCTA CTCTTTGACT CGACTCTGGA CTTAGAGATG 
AAGGAAAACC TCCAATTCCC TGATCGAAGT GTGCAAAGAA AGGTGTCTCC TGCCTCAGGT 
GTTTCCAAGA AGGTAGAACC CTCAGACCCA GTAGCCCGGC GTGTCTACCT TACGACACAA 
TTGAAACAAA AGAAGAGCAC ACTGGCATCA GTGAACATCC AGGCTCTTCC AGACAAGGGT 
CAAAAGTTGA TCAAACAAAT CCAGGAGCTG GAGGAAGTAC TCAGTGGTCT TGCCCTTTCC 
CCAGAGCAAG GAACTAATGA GAAGGGTAAC AGTCAAGTAC CACAGCAGAG TCACTTCACC 
AAAACTACCA CTGGCCCTCC CCACCTGGTG CCTCCCCAAC CCCTTCCTCA TCGTGGTACC 
CAACCTGTGG GTTCTCTAGA ACTAAAGTCT TCCTGCCAGG TGACTGCTGG AGGATCCAGT 
CAGTGCTATC AAGGCCATAC AAACCAAGAT CACGTTCATG CAGTGTGGAA AATCACAAGT 
GAAGCCATCG GTCAACTACA TCGCTCACTT GAGTCATGTC CTGGTGAAAT GGCTGTGGCA 
GAAGATCCCG CCGGGCTGAA GGTCCCTTTG CTACTACACC AGAAGCAGGC ATTGGCTTGG 
TTACTATGGC GGGAAAGTCA GAAGCCACAA GGAGGAATTC TGGCGGATGA TATGGGCTTA 
GGAAAAACCC TGACAATGAT TGCGCTCATC CTGACCCAGA AGAATCAAGA GAAAAAGAAA 
GAAAAGGAGA AAAGCACAGC TTTGACATGG CTCTCCAAAG ATGACTCTTC TGACTTTACT 
TCCCATGGAA CATTAATCAT CTGTCCTGCC TCCCTGATCC ATCATTGGAA AAATGAGGTG 
GAGAAACGGG TGAACAGCAA CAAACTAAGA GTCTATCTCT ACCATGGGCC AAACCGGGAT 
TCACGTGCCA GAGTCCTCTC TACATATGAC ATCGTGATCA CTACCTATAG CCTCGTGGCC 
AAGGAGATTC CCACAAGCAA GCAAAAGGCA GAGATCCCAG GTGCAAACCT CAATGTGGAG 
GGCACCTCAA CACCTTTGCT TCGAATAGCC TGGGCTCGAA TCATACTGGA TGAAGCTCAC 
AATGTTAAGA ATCCCCGAGT GCAGACGTCC ATAGCTGTGT GTAAGCTACA AGCCTGTGCC 
CGTTGGGCTG TCACTGGAAC CCCCATTCAA AACAACTTAT TGGATATGTA TTCACTGCTG 
AAGTTTCTCC GTTGCTCTCC ATTTGATGAG TTCAGTCTGT GGAGGAGTCA GGTTGACAAT 
GGCTCAAAGA AAGGAGGAGA ACGGTTAAGT ATTTTAACCA AGAGCCTTTT GCTGAGGAGA 
ACAAAAGACC AGCTGGACTC TACTGGCAGA CCTTTGGTGA TACTGCCCCA GCGTAAATTT 
CAGTTGCACC ATTTAAAGCT TTCTGAAGAT GAAGAGACTG TTTACAATGT GTTTTTTGCA 
AGATCAAGGT CAGCTCTGCA ATCCTATCTA AAAAGACATG AAAGTAGAGG CAACCAATCT 
GGAAGAAGCC CTAATAATCC ATTCAGTAGA GTGGCACTGG AGTTTGGGTC CGAGGAGCCT 
AGACACTCGG AGGCAGCCGA CTCACCGAGA TCCAGCACCG TCCACATACT GTCCCAGTTG 
CTGAGACTCC GCCAGTGTTG CTGTCATCTT TCTTTACTAA AGTCGGCCCT GGATCCAATG 
GAACTGAAGG GTGAAGGTCT TGTCCTTTCC CTGGAAGAAC AGCTCAGTGC TTTGACCTTG 
TCAGAACTCC GTGACTCAGA GCCATCTTCT ACTGTTTCCC TTAACGGCAC CTTCTTCAAG 
ATGGAGCTTT TTGAAGACAC GCGAGAGAGC ACCAAGATTT CATCTCTGTT GGCAGAATTG 
GAGGCAATTC AAAGAAATTC AGCATCCCAA AAGAGTGTCA TTGTCTCTCA GTGGACCAAC 
ATGCTGAAAG TTGTAGCATT GCACCTTAAG AAGCATGGAC TGACTTATGC CACCATCGAT 
GGCTCTGTCA ACCCCAAGCA GAGAATGGAC TTGGTAGAGG CATTTAACCA CTCCAGAGGC 
CCTCAGCTGT CTTCTAACAG GAATCCATCA CTTGAAGATC AAGCTTGTGA CCGAATTTAC 
CGAGTAGGGC AGCAGAAAGA TGTTGTCATA CACAAATTTG TTTGTGAGGG AACAGTAGAA 
GAAAAGATCT TACAGCTCCA AGAAAAAAAG AAAGATTTGG CCAAACAAGT TCTATCAGGG 
TCTGGAGAAT CTGTCACCAA GCTCACCTTG GCTGACCTCA GAGTCCTTTT TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002810426.2
CDS	70..3489
Translation	MEVVRCPEHGTFCFLKTGVRDGPNKGKSFYVCRADTCSFVRPTDIPVSHCLLHEDFVVELQGLLLPQDKKEYRLFFRCIRSKAEGKRWCGSIPWQDPDSKEHSVPNKSQHASVTFHHSSNWLRNPFKVLDKNQKPALWKQLIKGEGEEKTADKKQREKGDQLFDQKKEQKLETMEKDLSSGLVPKKKQSVVQEKKQEERAEIQCEAETGGTHKRDFSEIKSQQCQGNELTRPSASSQEKSSGKSQDVQRESEPLREKVTQLLPQNVHSPNSISKPQKGGPLNKEYTNWEAKETKAKDGPSTQATQKSLPRGHFQEQPETHGVPAPGGPAAQAAPAAPGLSLGEGREAATSSDDEEEDDVVFVSSKPGSPLLFDSTLDLEMKENLQFPDRSVQRKVSPASGVSKKVEPSDPVARRVYLTTQLKQKKSTLASVNIQALPDKGQKLIKQIQELEEVLSGLALSPEQGTNEKGNSQVPQQSHFTKTTTGPPHLVPPQPLPHRGTQPVGSLELKSSCQVTAGGSSQCYQGHTNQDHVHAVWKITSEAIGQLHRSLESCPGEMAVAEDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGLGKTLTMIALILTQKNQEKKKEKEKSTALTWLSKDDSSDFTSHGTLIICPASLIHHWKNEVEKRVNSNKLRVYLYHGPNRDSRARVLSTYDIVITTYSLVAKEIPTSKQKAEIPGANLNVEGTSTPLLRIAWARIILDEAHNVKNPRVQTSIAVCKLQACARWAVTGTPIQNNLLDMYSLLKFLRCSPFDEFSLWRSQVDNGSKKGGERLSILTKSLLLRRTKDQLDSTGRPLVILPQRKFQLHHLKLSEDEETVYNVFFARSRSALQSYLKRHESRGNQSGRSPNNPFSRVALEFGSEEPRHSEAADSPRSSTVHILSQLLRLRQCCCHLSLLKSALDPMELKGEGLVLSLEEQLSALTLSELRDSEPSSTVSLNGTFFKMELFEDTRESTKISSLLAELEAIQRNSASQKSVIVSQWTNMLKVVALHLKKHGLTYATIDGSVNPKQRMDLVEAFNHSRGPQLSSNRNPSLEDQACDRIYRVGQQKDVVIHKFVCEGTVEEKILQLQEKKKDLAKQVLSGSGESVTKLTLADLRVLFGI

Target ORF information:

RefSeq Version	XM_002810380.3
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii transcription termination factor, RNA polymerase II (TTF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002810380.3

ORF Insert Sequence:

ATGGAAGTAG TTAGGTGTCC AGAGCACGGG ACTTTCTGCT TTCTTAAGAC CGGCGTCCGC 
GATGGCCCTA ATAAAGGAAA GAGCTTCTAC GTGTGCCGGG CAGACACGTG CAGCTTCGTG 
CGGCCCACTG ACATTCCTGT TTCCCATTGC TTATTGCATG AGGACTTTGT GGTAGAGCTT 
CAGGGTTTGC TTCTGCCACA GGACAAGAAA GAATACAGAT TGTTCTTCCG ATGCATTAGA 
AGTAAGGCAG AGGGGAAACG CTGGTGTGGA AGTATTCCAT GGCAGGATCC TGATTCCAAA 
GAACATTCTG TACCCAATAA GTCTCAGCAT GCATCTGTGA CATTTCATCA TTCTTCCAAC 
TGGCTGAGAA ATCCATTCAA GGTACTTGAC AAGAATCAAA AACCAGCTCT CTGGAAACAG 
CTCATCAAAG GTGAAGGTGA GGAAAAGACG GCTGATAAGA AGCAAAGAGA AAAGGGAGAT 
CAGCTTTTTG ATCAAAAGAA GGAACAGAAG CTTGAAACGA TGGAGAAAGA TCTCTCATCT 
GGCCTGGTAC CAAAGAAAAA ACAATCTGTA GTTCAAGAGA AGAAGCAAGA AGAGAGAGCA 
GAGATTCAGT GTGAGGCAGA GACTGGAGGC ACACACAAAA GAGACTTTTC TGAAATTAAA 
TCTCAACAGT GCCAAGGTAA TGAGCTTACA AGACCATCTG CATCTTCTCA GGAGAAATCA 
AGTGGTAAGA GTCAAGATGT CCAAAGAGAA TCAGAACCTC TGAGAGAAAA GGTTACCCAG 
CTTTTGCCTC AAAATGTTCA CAGTCCCAAC TCAATAAGCA AGCCCCAGAA AGGGGGACCC 
CTCAACAAGG AGTACACGAA CTGGGAGGCT AAAGAAACAA AGGCAAAGGA TGGCCCTAGC 
ACACAGGCCA CCCAGAAAAG CCTGCCTCGG GGGCATTTCC AAGAGCAGCC GGAGACCCAC 
GGTGTGCCTG CTCCTGGAGG ACCAGCGGCT CAGGCTGCAC CAGCAGCACC AGGGCTTTCC 
CTGGGTGAGG GCCGTGAAGC TGCCACAAGC AGTGATGACG AGGAGGAAGA TGATGTTGTT 
TTTGTTTCCT CTAAGCCTGG GAGCCCCCTA CTCTTTGACT CGACTCTGGA CTTAGAGATG 
AAGGAAAACC TCCAATTCCC TGATCGAAGT GTGCAAAGAA AGGTGTCTCC TGCCTCAGGT 
GTTTCCAAGA AGGTAGAACC CTCAGACCCA GTAGCCCGGC GTGTCTACCT TACGACACAA 
TTGAAACAAA AGAAGAGCAC ACTGGCATCA GTGAACATCC AGGCTCTTCC AGACAAGGGT 
CAAAAGTTGA TCAAACAAAT CCAGGAGCTG GAGGAAGTAC TCAGTGGTCT TGCCCTTTCC 
CCAGAGCAAG GAACTAATGA GAAGGGTAAC AGTCAAGTAC CACAGCAGAG TCACTTCACC 
AAAACTACCA CTGGCCCTCC CCACCTGGTG CCTCCCCAAC CCCTTCCTCA TCGTGGTACC 
CAACCTGTGG GTTCTCTAGA ACTAAAGTCT TCCTGCCAGG TGACTGCTGG AGGATCCAGT 
CAGTGCTATC AAGGCCATAC AAACCAAGAT CACGTTCATG CAGTGTGGAA AATCACAAGT 
GAAGCCATCG GTCAACTACA TCGCTCACTT GAGTCATGTC CTGGTGAAAT GGCTGTGGCA 
GAAGATCCCG CCGGGCTGAA GGTCCCTTTG CTACTACACC AGAAGCAGGC ATTGGCTTGG 
TTACTATGGC GGGAAAGTCA GAAGCCACAA GGAGGAATTC TGGCGGATGA TATGGGCTTA 
GGAAAAACCC TGACAATGAT TGCGCTCATC CTGACCCAGA AGAATCAAGA GAAAAAGAAA 
GAAAAGGAGA AAAGCACAGC TTTGACATGG CTCTCCAAAG ATGACTCTTC TGACTTTACT 
TCCCATGGAA CATTAATCAT CTGTCCTGCC TCCCTGATCC ATCATTGGAA AAATGAGGTG 
GAGAAACGGG TGAACAGCAA CAAACTAAGA GTCTATCTCT ACCATGGGCC AAACCGGGAT 
TCACGTGCCA GAGTCCTCTC TACATATGAC ATCGTGATCA CTACCTATAG CCTCGTGGCC 
AAGGAGATTC CCACAAGCAA GCAAAAGGCA GAGATCCCAG GTGCAAACCT CAATGTGGAG 
GGCACCTCAA CACCTTTGCT TCGAATAGCC TGGGCTCGAA TCATACTGGA TGAAGCTCAC 
AATGTTAAGA ATCCCCGAGT GCAGACGTCC ATAGCTGTGT GTAAGCTACA AGCCTGTGCC 
CGTTGGGCTG TCACTGGAAC CCCCATTCAA AACAACTTAT TGGATATGTA TTCACTGCTG 
AAGTTTCTCC GTTGCTCTCC ATTTGATGAG TTCAGTCTGT GGAGGAGTCA GGTTGACAAT 
GGCTCAAAGA AAGGAGGAGA ACGGTTAAGT ATTTTAACCA AGAGCCTTTT GCTGAGGAGA 
ACAAAAGACC AGCTGGACTC TACTGGCAGA CCTTTGGTGA TACTGCCCCA GCGTAAATTT 
CAGTTGCACC ATTTAAAGCT TTCTGAAGAT GAAGAGACTG TTTACAATGT GTTTTTTGCA 
AGATCAAGGT CAGCTCTGCA ATCCTATCTA AAAAGACATG AAAGTAGAGG CAACCAATCT 
GGAAGAAGCC CTAATAATCC ATTCAGTAGA GTGGCACTGG AGTTTGGGTC CGAGGAGCCT 
AGACACTCGG AGGCAGCCGA CTCACCGAGA TCCAGCACCG TCCACATACT GTCCCAGTTG 
CTGAGACTCC GCCAGTGTTG CTGTCATCTT TCTTTACTAA AGTCGGCCCT GGATCCAATG 
GAACTGAAGG GTGAAGGTCT TGTCCTTTCC CTGGAAGAAC AGCTCAGTGC TTTGACCTTG 
TCAGAACTCC GTGACTCAGA GCCATCTTCT ACTGTTTCCC TTAACGGCAC CTTCTTCAAG 
ATGGAGCTTT TTGAAGACAC GCGAGAGAGC ACCAAGATTT CATCTCTGTT GGCAGAATTG 
GAGGCAATTC AAAGAAATTC AGCATCCCAA AAGAGTGTCA TTGTCTCTCA GTGGACCAAC 
ATGCTGAAAG TTGTAGCATT GCACCTTAAG AAGCATGGAC TGACTTATGC CACCATCGAT 
GGCTCTGTCA ACCCCAAGCA GAGAATGGAC TTGGTAGAGG CATTTAACCA CTCCAGAGGC 
CCTCAGCTGT CTTCTAACAG GAATCCATCA CTTGAAGATC AAGCTTGTGA CCGAATTTAC 
CGAGTAGGGC AGCAGAAAGA TGTTGTCATA CACAAATTTG TTTGTGAGGG AACAGTAGAA 
GAAAAGATCT TACAGCTCCA AGAAAAAAAG AAAGATTTGG CCAAACAAGT TCTATCAGGG 
TCTGGAGAAT CTGTCACCAA GCTCACCTTG GCTGACCTCA GAGTCCTTTT TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTF2 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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