Kcnj9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnj9
Entrez Gene ID	116560
Full Name	potassium voltage-gated channel subfamily J member 9
Synonyms	Girk3,Kir3.3
General protein information	Preferred Names potassium voltage-gated channel subfamily J member 9 Names G protein-activated inward rectifier potassium channel 3 GIRK-3 inward rectifier K(+) channel Kir3.3 inwardly rectifier K(+) channel Kir3.3 potassium channel, inwardly rectifying subfamily J member 9 potassium inwardly-rectifying channel, subfamily J, member 9
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q24
Summary	G-protein activated potassium channel [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053834.1	NP_446286.1	G protein-activated inward rectifier potassium channel 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	KCNJ9	NP_004974.2
	KCNJ9	XP_001117363.1
Canis lupus familiaris (dog)	KCNJ9	XP_005641003.1
Mus musculus (house mouse)	Kcnj9	NP_032455.2
Rattus norvegicus (Norway rat)	Kcnj9	NP_446286.1
Bos taurus (cattle)	KCNJ9	NP_001180173.1
Danio rerio (zebrafish)	LOC100334285	XP_002660948.1

Gene Ontology
Bibliography

Related articles in PubMed

Mechanism underlying selective regulation of G protein-gated inwardly rectifying potassium channels by the psychostimulant-sensitive sorting nexin 27.
Balana B, Maslennikov I, Kwiatkowski W, Stern KM, Bahima L, Choe S, Slesinger PA
Proceedings of the National Academy of Sciences of the United States of America108(14)5831-6(2011 Apr)

Functional consequences of the interactions among the neural cell adhesion molecule NCAM, the receptor tyrosine kinase TrkB, and the inwardly rectifying K+ channel KIR3.3.
Kleene R, Cassens C, B?hring R, Theis T, Xiao MF, Dityatev A, Schafer-Nielsen C, D?ring F, Wischmeyer E, Schachner M
The Journal of biological chemistry285(37)28968-79(2010 Sep)

Subcellular compartment-specific molecular diversity of pre- and post-synaptic GABA-activated GIRK channels in Purkinje cells.
Fern?ndez-Alacid L, Aguado C, Ciruela F, Mart?n R, Col?n J, Caba?ero MJ, Gassmann M, Watanabe M, Shigemoto R, Wickman K, Bettler B, S?nchez-Prieto J, Luj?n R
Journal of neurochemistry110(4)1363-76(2009 Aug)

Expression of Kir3.3 potassium channel subunits in supraependymal axons.
Pr?ss H, Derst C, Marinc C, Wenzel M, Veh RW
Neuroscience letters445(1)89-93(2008 Nov)

Distribution and neurochemical characterization of neurons expressing GIRK channels in the rat brain.
Saenz del Burgo L, Cortes R, Mengod G, Zarate J, Echevarria E, Salles J
The Journal of comparative neurology510(6)581-606(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a decisive role for the neuronal K(+) channel in regulating NCAM-dependent neurite outgrowth and attribute a physiologically meaningful role to the functional interplay of Kir3.3, NCAM, and TrkB in ontogeny
Title: Functional consequences of the interactions among the neural cell adhesion molecule NCAM, the receptor tyrosine kinase TrkB, and the inwardly rectifying K+ channel KIR3.3.

The Kir3.3 subunit protein is expressed in raphe-derived axons at the light and electron microscopic level.
Title: Expression of Kir3.3 potassium channel subunits in supraependymal axons.

Data describe localization of GIRK1, GIRK2, and GIRK3 subunits and 5-HT(1A) receptor in rat brain, and their co-expression with markers of glutamatergic, gamma-aminobutyric acid (GABA)ergic, cholinergic, and serotonergic neurons.
Title: Distribution and neurochemical characterization of neurons expressing GIRK channels in the rat brain.

The following Kcnj9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnj9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10804	NM_053834.1 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 9 (Kcnj9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnj9 ( NM_053834.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446286.1 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 9 (Kcnj9), mRNA.

Price & Availability↑

CloneID	ORa10804
Clone ID Related Accession (Same CDS sequence)	NM_053834.1
Accession Version	NM_053834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	G protein-activated inward rectifier potassium channel 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L77929.1. ##Evidence-Data-START## Transcript exon combination :: L77929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCAGG AGAACGCCGC TTTCTCTCCC GGGTCGGAGG AGCCACCGCG GCGCCGCGGT 
CGCCAGCGCT ACGTGGAGAA GGACGGGCGC TGCAACGTGC AGCAGGGCAA CGTCCGCGAG 
ACCTACCGCT ACCTGACCGA CCTGTTCACC ACGCTGGTAG ATCTGCAGTG GCGCCTCAGC 
CTGCTCTTCT TCGTGCTCGC CTACGCGCTC ACTTGGCTCT TCTTCGGCGC CATCTGGTGG 
CTCATCGCCT ACGGCCGCGG CGACCTGGAG CACCTGGAGG ACACCGCGTG GACACCGTGC 
GTCAACAACC TCAACGGCTT CGTGGCCGCC TTCCTCTTCT CCATCGAGAC AGAAACCACC 
ATCGGCTATG GGCACCGTGT CATCACCGAC CAGTGCCCCG AGGGCATCGT GCTGCTGCTG 
TTGCAAGCCA TCCTGGGCTC CATGGTGAAC GCTTTCATGG TGGGCTGCAT GTTCGTCAAG 
ATCTCGCAGC CCAACAAGCG CGCCGCCACG CTCGTCTTCT CCTCGCACGC CGTGGTGTCT 
CTGCGCGACG GGCGCCTCTG TCTCATGTTT CGCGTGGGGG ACCTGCGATC CTCGCACATC 
GTCGAGGCCT CCATCCGCGC CAAGCTCATC CGCTCGCGCC AGACGCTCGA GGGCGAGTTC 
ATCCCTCTGC ACCAGACCGA CCTCAGCGTG GGCTTCGACA CGGGGGACGA CCGCCTCTTC 
CTCGTCTCAC CTCTCGTCAT CAGCCATGAA ATCGACGCCG CCAGCCCCTT CTGGGAGGCA 
TCGCGCCGCG CCCTCGAGAG GGACGACTTC GAGATCGTAG TCATTCTCGA GGGCATGGTG 
GAAGCCACGG GAATGACGTG CCAAGCTCGA AGCTCGTACC TGGTAGACGA AGTGTTGTGG 
GGCCACCGAT TCACATCCGT GCTCACCCTG GAGGACGGTT TCTATGAGGT GGACTACGCT 
AGCTTCCATG AAACCTTTGA GGTGCCCACA CCCTCATGCA GTGCTCGGGA ACTGGCAGAA 
GCTGCAGCCC GCCTTGACGC CCATCTCTAC TGGTCCATCC CCAGCAGGCT GGATGAGAAG 
GTGGAGGAAG AAGGGGCTGG GGAGGGGGCA GGCGCAGGAG ATGGAGCTGA CAAGGAGCAA 
AATGGCTGCC TGCCACCCCC GGAGAGTGAG TCCAAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446286.1
CDS	252..1433
Translation	MAQENAAFSPGSEEPPRRRGRQRYVEKDGRCNVQQGNVRETYRYLTDLFTTLVDLQWRLSLLFFVLAYALTWLFFGAIWWLIAYGRGDLEHLEDTAWTPCVNNLNGFVAAFLFSIETETTIGYGHRVITDQCPEGIVLLLLQAILGSMVNAFMVGCMFVKISQPNKRAATLVFSSHAVVSLRDGRLCLMFRVGDLRSSHIVEASIRAKLIRSRQTLEGEFIPLHQTDLSVGFDTGDDRLFLVSPLVISHEIDAASPFWEASRRALERDDFEIVVILEGMVEATGMTCQARSSYLVDEVLWGHRFTSVLTLEDGFYEVDYASFHETFEVPTPSCSARELAEAAARLDAHLYWSIPSRLDEKVEEEGAGEGAGAGDGADKEQNGCLPPPESESKV

Target ORF information:

RefSeq Version	NM_053834.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 9 (Kcnj9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053834.1

ORF Insert Sequence:

ATGGCGCAGG AGAACGCCGC TTTCTCTCCC GGGTCGGAGG AGCCACCGCG GCGCCGCGGT 
CGCCAGCGCT ACGTGGAGAA GGACGGGCGC TGCAACGTGC AGCAGGGCAA CGTCCGCGAG 
ACCTACCGCT ACCTGACCGA CCTGTTCACC ACGCTGGTAG ATCTGCAGTG GCGCCTCAGC 
CTGCTCTTCT TCGTGCTCGC CTACGCGCTC ACTTGGCTCT TCTTCGGCGC CATCTGGTGG 
CTCATCGCCT ACGGCCGCGG CGACCTGGAG CACCTGGAGG ACACCGCGTG GACACCGTGC 
GTCAACAACC TCAACGGCTT CGTGGCCGCC TTCCTCTTCT CCATCGAGAC AGAAACCACC 
ATCGGCTATG GGCACCGTGT CATCACCGAC CAGTGCCCCG AGGGCATCGT GCTGCTGCTG 
TTGCAAGCCA TCCTGGGCTC CATGGTGAAC GCTTTCATGG TGGGCTGCAT GTTCGTCAAG 
ATCTCGCAGC CCAACAAGCG CGCCGCCACG CTCGTCTTCT CCTCGCACGC CGTGGTGTCT 
CTGCGCGACG GGCGCCTCTG TCTCATGTTT CGCGTGGGGG ACCTGCGATC CTCGCACATC 
GTCGAGGCCT CCATCCGCGC CAAGCTCATC CGCTCGCGCC AGACGCTCGA GGGCGAGTTC 
ATCCCTCTGC ACCAGACCGA CCTCAGCGTG GGCTTCGACA CGGGGGACGA CCGCCTCTTC 
CTCGTCTCAC CTCTCGTCAT CAGCCATGAA ATCGACGCCG CCAGCCCCTT CTGGGAGGCA 
TCGCGCCGCG CCCTCGAGAG GGACGACTTC GAGATCGTAG TCATTCTCGA GGGCATGGTG 
GAAGCCACGG GAATGACGTG CCAAGCTCGA AGCTCGTACC TGGTAGACGA AGTGTTGTGG 
GGCCACCGAT TCACATCCGT GCTCACCCTG GAGGACGGTT TCTATGAGGT GGACTACGCT 
AGCTTCCATG AAACCTTTGA GGTGCCCACA CCCTCATGCA GTGCTCGGGA ACTGGCAGAA 
GCTGCAGCCC GCCTTGACGC CCATCTCTAC TGGTCCATCC CCAGCAGGCT GGATGAGAAG 
GTGGAGGAAG AAGGGGCTGG GGAGGGGGCA GGCGCAGGAG ATGGAGCTGA CAAGGAGCAA 
AATGGCTGCC TGCCACCCCC GGAGAGTGAG TCCAAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanism underlying selective regulation of G protein-gated inwardly rectifying potassium channels by the psychostimulant-sensitive sorting nexin 27.
Proceedings of the National Academy of Sciences of the United States of America108(14)5831-6(2011 Apr)
Balana B,Maslennikov I,Kwiatkowski W,Stern KM,Bahima L,Choe S,Slesinger PA

Functional consequences of the interactions among the neural cell adhesion molecule NCAM, the receptor tyrosine kinase TrkB, and the inwardly rectifying K+ channel KIR3.3.
The Journal of biological chemistry285(37)28968-79(2010 Sep)
Kleene R,Cassens C,B?hring R,Theis T,Xiao MF,Dityatev A,Schafer-Nielsen C,D?ring F,Wischmeyer E,Schachner M

Subcellular compartment-specific molecular diversity of pre- and post-synaptic GABA-activated GIRK channels in Purkinje cells.
Journal of neurochemistry110(4)1363-76(2009 Aug)
Fern?ndez-Alacid L,Aguado C,Ciruela F,Mart?n R,Col?n J,Caba?ero MJ,Gassmann M,Watanabe M,Shigemoto R,Wickman K,Bettler B,S?nchez-Prieto J,Luj?n R

Expression of Kir3.3 potassium channel subunits in supraependymal axons.
Neuroscience letters445(1)89-93(2008 Nov)
Pr?ss H,Derst C,Marinc C,Wenzel M,Veh RW

Distribution and neurochemical characterization of neurons expressing GIRK channels in the rat brain.
The Journal of comparative neurology510(6)581-606(2008 Oct)
Saenz del Burgo L,Cortes R,Mengod G,Zarate J,Echevarria E,Salles J

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Kcnj9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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