Slc9a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc9a2
Entrez Gene ID	24783
Full Name	solute carrier family 9 member A2
Synonyms	Nhe2
General protein information	Preferred Names solute carrier family 9 member A2 Names sodium/hydrogen exchanger 2 H7 NHE-2 Na(+)/H(+) exchanger 2 Na/H ion exchanger Solute carrier family 9 (sodium/hydrogen exchanger 2), antiporter 2, Na+/H+ (Na+/H+ exchanger 2) solute carrier family 9 (sodium/hydrogen exchanger), member 2 solute carrier family 9 member 2 solute carrier family 9, subfamily A (NHE2, cation proton antiporter 2), member 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q22
Summary	displays Na+/H+ exchange activity; may play a role in apical membrane Na(+)/H(+) exchange in the distal convoluted tubule of the kidney [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012653.2	NP_036785.2	sodium/hydrogen exchanger 2 isoform 2
NM_001113335.1	NP_001106806.1	sodium/hydrogen exchanger 2 isoform 1 precursor

Pathways from BioSystems

Homologies

	SLC9A2	XP_001108368.1
Danio rerio (zebrafish)	slc9a2	NP_001107567.1
Pan troglodytes (chimpanzee)	SLC9A2	XP_525838.2
Gallus gallus (chicken)	SLC9A2	XP_416918.4
Mus musculus (house mouse)	Slc9a2	NP_001028461.2
Homo sapiens (human)	SLC9A2	NP_003039.2
Canis lupus familiaris (dog)	SLC9A2	XP_005626049.1
Bos taurus (cattle)	SLC9A2	XP_002691231.3
Rattus norvegicus (Norway rat)	Slc9a2	NP_001106806.1
Xenopus tropicalis (tropical clawed frog)	LOC101732511	XP_004911848.1

Gene Ontology
Bibliography

Related articles in PubMed

Na
Paehler Vor der Nolte A, Chodisetti G, Yuan Z, Busch F, Riederer B, Luo M, Yu Y, Menon MB, Schneider A, Stripecke R, Nikolovska K, Yeruva S, Seidler U
Journal of cellular physiology232(7)1669-1680(2017 Jul)

AQP2-Induced Acceleration of Renal Cell Proliferation Involves the Activation of a Regulatory Volume Increase Mechanism Dependent on NHE2.
Rivarola V, Di Giusto G, Christensen MJ, Ford P, Capurro C
Journal of cellular biochemistry118(5)967-978(2017 May)

Mechanism of down regulation of Na-H exchanger-2 in experimental colitis.
Soleiman AA, Thameem F, Khan I
PloS one12(5)e0176767(2017)

Na-H Exchanger Isoform-2 (NHE2) Mediates Butyrate-dependent Na+ Absorption in Dextran Sulfate Sodium (DSS)-induced Colitis.
Rajendran VM, Nanda Kumar NS, Tse CM, Binder HJ
The Journal of biological chemistry290(42)25487-96(2015 Oct)

Enhanced Expression of Sodium Hydrogen Exchanger (NHE)-1, 2 and 4 in the Cervix of Ovariectomised Rats by Phytoestrogen Genistein.
Ismail N, Giribabu N, Muniandy S, Salleh N
International journal of medical sciences12(6)468-77(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results demonstrate that in RGM1 cells, migratory velocity is increased by NHE1 activation, while NHE2 activity inhibit this process.
Title: Na

Results provide evidence that NHE2 and AQP2 work in coordination as part of the cellular device that regulates collecting duct cell cycle progression.
Title: AQP2-Induced Acceleration of Renal Cell Proliferation Involves the Activation of a Regulatory Volume Increase Mechanism Dependent on NHE2.

These findings demonstrate suppression of NHE-2 expression on the brush borders in the colonic epithelial cells which is regulated transcriptionally.
Title: Mechanism of down regulation of Na-H exchanger-2 in experimental colitis.

observations suggest that the butyrate activation of NHE2 would be a potential target to control UC-associated diarrhea
Title: Na-H Exchanger Isoform-2 (NHE2) Mediates Butyrate-dependent Na+ Absorption in Dextran Sulfate Sodium (DSS)-induced Colitis.

Enhanced expression of NHE-1, 2 and 4 proteins and mRNAs expression in cervix under genistein influence could help to restore the cervicovaginal fluid pH that might help to prevent cervicovaginal complications related to menopause
Title: Enhanced Expression of Sodium Hydrogen Exchanger (NHE)-1, 2 and 4 in the Cervix of Ovariectomised Rats by Phytoestrogen Genistein.

The following Slc9a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc9a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00545	NM_012653.2 Latest version!	Rattus norvegicus solute carrier family 9 member A2 (Slc9a2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa00942	NM_001113335.1 Latest version!	Rattus norvegicus solute carrier family 9 member A2 (Slc9a2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc9a2 ( NM_012653.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036785.2 Rattus norvegicus solute carrier family 9 member A2 (Slc9a2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa00545
Clone ID Related Accession (Same CDS sequence)	NM_012653.2
Accession Version	NM_012653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	sodium/hydrogen exchanger 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L11004.1, L11236.1 and BE117286.1. On Jan 9, 2008 this sequence version replaced NM_012653.1. Transcript Variant: This variant (2) lacks the 5' exon and has an alternate 5' segment, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L11004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689595, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGGTTGGGC TTCTACTAGG TGGGATTATC TTCGGTGTCG ATGAGAAGTC TCCTCCTGCA 
ATGAAAACGG ATGTATTTTT CCTATACCTC CTGCCACCCA TTGTTCTGGA TGCTGGCTAC 
TTCATGCCTA CTCGACCTTT CTTTGAGAAC TTGGGCACCA TTTTCTGGTA TGCTGTGGTA 
GGCACACTGT GGAATTCCAT TGGCATTGGG CTGTCTTTGT TCGGCATCTG CCAGATTGAG 
GCGTTTGGCC TCAGCGACAT CACCTTGCTC CAGAACCTGC TCTTCGGCAG CCTGATCTCA 
GCTGTGGACC CTGTGGCTGT GCTTGCTGTC TTTGAGAACA TTCATGTCAA TGAACAGCTC 
TATATCCTGG TTTTTGGCGA GTCTCTGCTC AACGATGCAG TCACAGTGGT CCTGTACAAC 
TTGTTCAAAT CCTTCTGCCA GATGAAGACC ATCCAGACCG TAGACGTGTT TGCTGGCATC 
GCTAACTTCT TCGTAGTGGG GATTGGCGGG GTGCTGATTG GTATCCTGCT GGGATTCATA 
GCAGCGTTCA CCACCCGTTT CACACACAAC ATCCGGGTCA TTGAGCCGCT CTTTGTCTTC 
CTGTACAGCT ACTTGTCCTA CATCACTGCT GAAATGTTCC ACCTTTCAGG CATCATGGCG 
ATCACAGCCT GTGCCATGAC GATGAACAAG TATGTGGAAG AGAACGTGTC CCAGAAGTCC 
TACACGACCA TCAAGTACTT CATGAAGATG CTGAGCAGCG TGAGTGAGAC CCTCATCTTC 
ATCTTCATGG GCGTGTCCAC CGTTGGGAAG AACCATGAGT GGAACTGGGC TTTCGTCTGC 
TTCACCCTGG CCTTCTGCCT GATCTGGCGA GCACTGGGTG TCTTTGTCCT GACTCAGGTG 
ATTAATTGGT TCCGGACCAT CCCACTGACC TTCAAGGACC AGTTCATCAT TGCCTATGGA 
GGGCTCCGCG GGGCCATCTG CTTTGCACTG GTGTTCCTCC TCCCTGCTAC TGTGTTTCCC 
CGAAAGAAGC TGTTCATCAC AGCTGCCATC GTCGTTATAT TCTTCACTGT CTTCATCCTG 
GGAATAACCA TCCGGCCACT GGTGGAATTT CTTGATGTTA AGAGATCCAA TAAGAAGCAG 
CAAGCTGTCA GTGAAGAGAT CCACTGTCGG TTTTTTGATC ATGTGAAGAC TGGGATTGAA 
GATGTTTGTG GACACTGGGG TCACAACTTC TGGAGAGACA AGTTTAAGAA ATTTGATGAC 
AAATACCTTC GGAAGCTTCT GATTCGGGAA AACCAACCCA AGTCTAGCAT TGTGTCCTTA 
TATAAAAAGC TTGAAATAAA ACATGCCATA GAGATGGCAG AGACAGGGAT GATAAGCACT 
GTCCCCTCAT TTGCATCTCT CAACGACTGC CGTGAAGAGA AAATAAGGAA GCTCACGCCA 
GGAGAAATGG ATGAAATTAG AGAGATATTG TCAAGGAATC TCTATCAAAT CCGCCAGCGG 
ACCTTGTCCT ACAACAGACA CAACCTGACG GCAGACACAA GTGAGAGGCA AGCCAAGGAG 
ATCTTGATTC GCCGACGGCA CAGTCTTCGG GAAAGTCTTA GGAAGGACAA CAGCTTAAAC 
CGCGAGCGCA GGGCTTCTAC TTCAACCTCC CGATATTTAT CCTTACCTAA AAACACAAAG 
CTTCCCGAGA AGTTGCAGAA GAAGAATAAG GTTTCAAATG CAGATGGTAA TAGCAGCGAC 
TCGGACATGG ATGGAACCAC TGTACTCAAT TTGCAGCCCA GGGCCAGGCG CTTCTTGCCA 
GATCAGTTCT CAAAGAAAGC CTCACCAGCC TACAAAATGG AATGGAAGAA TGAGGTGGAC 
GTGGGTTCTG CGCGAGCGCC CCCCAGCGTC ACTCCAGCGC CTCGCAGTAA AGAGGGGGGC 
ACCCAGACAC CAGGGGTGCT GAGGCAGCCC CTGCTCTCCA AAGACCAACG GTTTGGCCGG 
GGCAGGGAAG ACAGTTTGAC TGAAGATGTT CCACCCAAGC CCCCACCAAG GTTGGTGCGA 
CGAGCATCAG AACCCGGAAA TAGGAAAGGC CGCCTTGGCA ACGAAAAGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036785.2
CDS	532..2625
Translation	MVGLLLGGIIFGVDEKSPPAMKTDVFFLYLLPPIVLDAGYFMPTRPFFENLGTIFWYAVVGTLWNSIGIGLSLFGICQIEAFGLSDITLLQNLLFGSLISAVDPVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIQTVDVFAGIANFFVVGIGGVLIGILLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGIMAITACAMTMNKYVEENVSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFVCFTLAFCLIWRALGVFVLTQVINWFRTIPLTFKDQFIIAYGGLRGAICFALVFLLPATVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVKRSNKKQQAVSEEIHCRFFDHVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKLEIKHAIEMAETGMISTVPSFASLNDCREEKIRKLTPGEMDEIREILSRNLYQIRQRTLSYNRHNLTADTSERQAKEILIRRRHSLRESLRKDNSLNRERRASTSTSRYLSLPKNTKLPEKLQKKNKVSNADGNSSDSDMDGTTVLNLQPRARRFLPDQFSKKASPAYKMEWKNEVDVGSARAPPSVTPAPRSKEGGTQTPGVLRQPLLSKDQRFGRGREDSLTEDVPPKPPPRLVRRASEPGNRKGRLGNEKP

Target ORF information:

RefSeq Version	NM_012653.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 9 member A2 (Slc9a2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012653.2

ORF Insert Sequence:

ATGGTTGGGC TTCTACTAGG TGGGATTATC TTCGGTGTCG ATGAGAAGTC TCCTCCTGCA 
ATGAAAACGG ATGTATTTTT CCTATACCTC CTGCCACCCA TTGTTCTGGA TGCTGGCTAC 
TTCATGCCTA CTCGACCTTT CTTTGAGAAC TTGGGCACCA TTTTCTGGTA TGCTGTGGTA 
GGCACACTGT GGAATTCCAT TGGCATTGGG CTGTCTTTGT TCGGCATCTG CCAGATTGAG 
GCGTTTGGCC TCAGCGACAT CACCTTGCTC CAGAACCTGC TCTTCGGCAG CCTGATCTCA 
GCTGTGGACC CTGTGGCTGT GCTTGCTGTC TTTGAGAACA TTCATGTCAA TGAACAGCTC 
TATATCCTGG TTTTTGGCGA GTCTCTGCTC AACGATGCAG TCACAGTGGT CCTGTACAAC 
TTGTTCAAAT CCTTCTGCCA GATGAAGACC ATCCAGACCG TAGACGTGTT TGCTGGCATC 
GCTAACTTCT TCGTAGTGGG GATTGGCGGG GTGCTGATTG GTATCCTGCT GGGATTCATA 
GCAGCGTTCA CCACCCGTTT CACACACAAC ATCCGGGTCA TTGAGCCGCT CTTTGTCTTC 
CTGTACAGCT ACTTGTCCTA CATCACTGCT GAAATGTTCC ACCTTTCAGG CATCATGGCG 
ATCACAGCCT GTGCCATGAC GATGAACAAG TATGTGGAAG AGAACGTGTC CCAGAAGTCC 
TACACGACCA TCAAGTACTT CATGAAGATG CTGAGCAGCG TGAGTGAGAC CCTCATCTTC 
ATCTTCATGG GCGTGTCCAC CGTTGGGAAG AACCATGAGT GGAACTGGGC TTTCGTCTGC 
TTCACCCTGG CCTTCTGCCT GATCTGGCGA GCACTGGGTG TCTTTGTCCT GACTCAGGTG 
ATTAATTGGT TCCGGACCAT CCCACTGACC TTCAAGGACC AGTTCATCAT TGCCTATGGA 
GGGCTCCGCG GGGCCATCTG CTTTGCACTG GTGTTCCTCC TCCCTGCTAC TGTGTTTCCC 
CGAAAGAAGC TGTTCATCAC AGCTGCCATC GTCGTTATAT TCTTCACTGT CTTCATCCTG 
GGAATAACCA TCCGGCCACT GGTGGAATTT CTTGATGTTA AGAGATCCAA TAAGAAGCAG 
CAAGCTGTCA GTGAAGAGAT CCACTGTCGG TTTTTTGATC ATGTGAAGAC TGGGATTGAA 
GATGTTTGTG GACACTGGGG TCACAACTTC TGGAGAGACA AGTTTAAGAA ATTTGATGAC 
AAATACCTTC GGAAGCTTCT GATTCGGGAA AACCAACCCA AGTCTAGCAT TGTGTCCTTA 
TATAAAAAGC TTGAAATAAA ACATGCCATA GAGATGGCAG AGACAGGGAT GATAAGCACT 
GTCCCCTCAT TTGCATCTCT CAACGACTGC CGTGAAGAGA AAATAAGGAA GCTCACGCCA 
GGAGAAATGG ATGAAATTAG AGAGATATTG TCAAGGAATC TCTATCAAAT CCGCCAGCGG 
ACCTTGTCCT ACAACAGACA CAACCTGACG GCAGACACAA GTGAGAGGCA AGCCAAGGAG 
ATCTTGATTC GCCGACGGCA CAGTCTTCGG GAAAGTCTTA GGAAGGACAA CAGCTTAAAC 
CGCGAGCGCA GGGCTTCTAC TTCAACCTCC CGATATTTAT CCTTACCTAA AAACACAAAG 
CTTCCCGAGA AGTTGCAGAA GAAGAATAAG GTTTCAAATG CAGATGGTAA TAGCAGCGAC 
TCGGACATGG ATGGAACCAC TGTACTCAAT TTGCAGCCCA GGGCCAGGCG CTTCTTGCCA 
GATCAGTTCT CAAAGAAAGC CTCACCAGCC TACAAAATGG AATGGAAGAA TGAGGTGGAC 
GTGGGTTCTG CGCGAGCGCC CCCCAGCGTC ACTCCAGCGC CTCGCAGTAA AGAGGGGGGC 
ACCCAGACAC CAGGGGTGCT GAGGCAGCCC CTGCTCTCCA AAGACCAACG GTTTGGCCGG 
GGCAGGGAAG ACAGTTTGAC TGAAGATGTT CCACCCAAGC CCCCACCAAG GTTGGTGCGA 
CGAGCATCAG AACCCGGAAA TAGGAAAGGC CGCCTTGGCA ACGAAAAGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc9a2 ( NM_001113335.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001106806.1 Rattus norvegicus solute carrier family 9 member A2 (Slc9a2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa00942
Clone ID Related Accession (Same CDS sequence)	NM_001113335.1
Accession Version	NM_001113335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	sodium/hydrogen exchanger 2 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L11236.1, L11004.1 and BE117286.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689595, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCCCT CAGGCACTGC GCACAGAATG CGGGCGCCAC TGTCTTGGCT GCTGCTGCTG 
CTGCTGTCTC TGCAGGTGGC AGTGCCGGCG GGCGCACTGG CTGAGACCCT GCTGGACGCA 
CCCGGGGCCA GGGGTGCCAG CTCCAACCCG CCAAGCCCCG CCAGTGTGGT GGCCCCGGGG 
ACAACGCCGT TCGAGGAAAG TCGATTGCCC GTGTTCACCC TGGATTACCC CCACGTGCAA 
ATCCCTTTCG AGATCACTTT GTGGATCCTA CTGGCTTCCT TGGCCAAGAT TGGTTTCCAT 
CTCTATCACA AGTTGCCCAC GATTGTGCCG GAGAGCTGTC TTCTCATAAT GGTTGGGCTT 
CTACTAGGTG GGATTATCTT CGGTGTCGAT GAGAAGTCTC CTCCTGCAAT GAAAACGGAT 
GTATTTTTCC TATACCTCCT GCCACCCATT GTTCTGGATG CTGGCTACTT CATGCCTACT 
CGACCTTTCT TTGAGAACTT GGGCACCATT TTCTGGTATG CTGTGGTAGG CACACTGTGG 
AATTCCATTG GCATTGGGCT GTCTTTGTTC GGCATCTGCC AGATTGAGGC GTTTGGCCTC 
AGCGACATCA CCTTGCTCCA GAACCTGCTC TTCGGCAGCC TGATCTCAGC TGTGGACCCT 
GTGGCTGTGC TTGCTGTCTT TGAGAACATT CATGTCAATG AACAGCTCTA TATCCTGGTT 
TTTGGCGAGT CTCTGCTCAA CGATGCAGTC ACAGTGGTCC TGTACAACTT GTTCAAATCC 
TTCTGCCAGA TGAAGACCAT CCAGACCGTA GACGTGTTTG CTGGCATCGC TAACTTCTTC 
GTAGTGGGGA TTGGCGGGGT GCTGATTGGT ATCCTGCTGG GATTCATAGC AGCGTTCACC 
ACCCGTTTCA CACACAACAT CCGGGTCATT GAGCCGCTCT TTGTCTTCCT GTACAGCTAC 
TTGTCCTACA TCACTGCTGA AATGTTCCAC CTTTCAGGCA TCATGGCGAT CACAGCCTGT 
GCCATGACGA TGAACAAGTA TGTGGAAGAG AACGTGTCCC AGAAGTCCTA CACGACCATC 
AAGTACTTCA TGAAGATGCT GAGCAGCGTG AGTGAGACCC TCATCTTCAT CTTCATGGGC 
GTGTCCACCG TTGGGAAGAA CCATGAGTGG AACTGGGCTT TCGTCTGCTT CACCCTGGCC 
TTCTGCCTGA TCTGGCGAGC ACTGGGTGTC TTTGTCCTGA CTCAGGTGAT TAATTGGTTC 
CGGACCATCC CACTGACCTT CAAGGACCAG TTCATCATTG CCTATGGAGG GCTCCGCGGG 
GCCATCTGCT TTGCACTGGT GTTCCTCCTC CCTGCTACTG TGTTTCCCCG AAAGAAGCTG 
TTCATCACAG CTGCCATCGT CGTTATATTC TTCACTGTCT TCATCCTGGG AATAACCATC 
CGGCCACTGG TGGAATTTCT TGATGTTAAG AGATCCAATA AGAAGCAGCA AGCTGTCAGT 
GAAGAGATCC ACTGTCGGTT TTTTGATCAT GTGAAGACTG GGATTGAAGA TGTTTGTGGA 
CACTGGGGTC ACAACTTCTG GAGAGACAAG TTTAAGAAAT TTGATGACAA ATACCTTCGG 
AAGCTTCTGA TTCGGGAAAA CCAACCCAAG TCTAGCATTG TGTCCTTATA TAAAAAGCTT 
GAAATAAAAC ATGCCATAGA GATGGCAGAG ACAGGGATGA TAAGCACTGT CCCCTCATTT 
GCATCTCTCA ACGACTGCCG TGAAGAGAAA ATAAGGAAGC TCACGCCAGG AGAAATGGAT 
GAAATTAGAG AGATATTGTC AAGGAATCTC TATCAAATCC GCCAGCGGAC CTTGTCCTAC 
AACAGACACA ACCTGACGGC AGACACAAGT GAGAGGCAAG CCAAGGAGAT CTTGATTCGC 
CGACGGCACA GTCTTCGGGA AAGTCTTAGG AAGGACAACA GCTTAAACCG CGAGCGCAGG 
GCTTCTACTT CAACCTCCCG ATATTTATCC TTACCTAAAA ACACAAAGCT TCCCGAGAAG 
TTGCAGAAGA AGAATAAGGT TTCAAATGCA GATGGTAATA GCAGCGACTC GGACATGGAT 
GGAACCACTG TACTCAATTT GCAGCCCAGG GCCAGGCGCT TCTTGCCAGA TCAGTTCTCA 
AAGAAAGCCT CACCAGCCTA CAAAATGGAA TGGAAGAATG AGGTGGACGT GGGTTCTGCG 
CGAGCGCCCC CCAGCGTCAC TCCAGCGCCT CGCAGTAAAG AGGGGGGCAC CCAGACACCA 
GGGGTGCTGA GGCAGCCCCT GCTCTCCAAA GACCAACGGT TTGGCCGGGG CAGGGAAGAC 
AGTTTGACTG AAGATGTTCC ACCCAAGCCC CCACCAAGGT TGGTGCGACG AGCATCAGAA 
CCCGGAAATA GGAAAGGCCG CCTTGGCAAC GAAAAGCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106806.1
CDS	187..2628
Translation	MGPSGTAHRMRAPLSWLLLLLLSLQVAVPAGALAETLLDAPGARGASSNPPSPASVVAPGTTPFEESRLPVFTLDYPHVQIPFEITLWILLASLAKIGFHLYHKLPTIVPESCLLIMVGLLLGGIIFGVDEKSPPAMKTDVFFLYLLPPIVLDAGYFMPTRPFFENLGTIFWYAVVGTLWNSIGIGLSLFGICQIEAFGLSDITLLQNLLFGSLISAVDPVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIQTVDVFAGIANFFVVGIGGVLIGILLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGIMAITACAMTMNKYVEENVSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFVCFTLAFCLIWRALGVFVLTQVINWFRTIPLTFKDQFIIAYGGLRGAICFALVFLLPATVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVKRSNKKQQAVSEEIHCRFFDHVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKLEIKHAIEMAETGMISTVPSFASLNDCREEKIRKLTPGEMDEIREILSRNLYQIRQRTLSYNRHNLTADTSERQAKEILIRRRHSLRESLRKDNSLNRERRASTSTSRYLSLPKNTKLPEKLQKKNKVSNADGNSSDSDMDGTTVLNLQPRARRFLPDQFSKKASPAYKMEWKNEVDVGSARAPPSVTPAPRSKEGGTQTPGVLRQPLLSKDQRFGRGREDSLTEDVPPKPPPRLVRRASEPGNRKGRLGNEKP

Target ORF information:

RefSeq Version	NM_001113335.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 9 member A2 (Slc9a2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113335.1

ORF Insert Sequence:

ATGGGCCCCT CAGGCACTGC GCACAGAATG CGGGCGCCAC TGTCTTGGCT GCTGCTGCTG 
CTGCTGTCTC TGCAGGTGGC AGTGCCGGCG GGCGCACTGG CTGAGACCCT GCTGGACGCA 
CCCGGGGCCA GGGGTGCCAG CTCCAACCCG CCAAGCCCCG CCAGTGTGGT GGCCCCGGGG 
ACAACGCCGT TCGAGGAAAG TCGATTGCCC GTGTTCACCC TGGATTACCC CCACGTGCAA 
ATCCCTTTCG AGATCACTTT GTGGATCCTA CTGGCTTCCT TGGCCAAGAT TGGTTTCCAT 
CTCTATCACA AGTTGCCCAC GATTGTGCCG GAGAGCTGTC TTCTCATAAT GGTTGGGCTT 
CTACTAGGTG GGATTATCTT CGGTGTCGAT GAGAAGTCTC CTCCTGCAAT GAAAACGGAT 
GTATTTTTCC TATACCTCCT GCCACCCATT GTTCTGGATG CTGGCTACTT CATGCCTACT 
CGACCTTTCT TTGAGAACTT GGGCACCATT TTCTGGTATG CTGTGGTAGG CACACTGTGG 
AATTCCATTG GCATTGGGCT GTCTTTGTTC GGCATCTGCC AGATTGAGGC GTTTGGCCTC 
AGCGACATCA CCTTGCTCCA GAACCTGCTC TTCGGCAGCC TGATCTCAGC TGTGGACCCT 
GTGGCTGTGC TTGCTGTCTT TGAGAACATT CATGTCAATG AACAGCTCTA TATCCTGGTT 
TTTGGCGAGT CTCTGCTCAA CGATGCAGTC ACAGTGGTCC TGTACAACTT GTTCAAATCC 
TTCTGCCAGA TGAAGACCAT CCAGACCGTA GACGTGTTTG CTGGCATCGC TAACTTCTTC 
GTAGTGGGGA TTGGCGGGGT GCTGATTGGT ATCCTGCTGG GATTCATAGC AGCGTTCACC 
ACCCGTTTCA CACACAACAT CCGGGTCATT GAGCCGCTCT TTGTCTTCCT GTACAGCTAC 
TTGTCCTACA TCACTGCTGA AATGTTCCAC CTTTCAGGCA TCATGGCGAT CACAGCCTGT 
GCCATGACGA TGAACAAGTA TGTGGAAGAG AACGTGTCCC AGAAGTCCTA CACGACCATC 
AAGTACTTCA TGAAGATGCT GAGCAGCGTG AGTGAGACCC TCATCTTCAT CTTCATGGGC 
GTGTCCACCG TTGGGAAGAA CCATGAGTGG AACTGGGCTT TCGTCTGCTT CACCCTGGCC 
TTCTGCCTGA TCTGGCGAGC ACTGGGTGTC TTTGTCCTGA CTCAGGTGAT TAATTGGTTC 
CGGACCATCC CACTGACCTT CAAGGACCAG TTCATCATTG CCTATGGAGG GCTCCGCGGG 
GCCATCTGCT TTGCACTGGT GTTCCTCCTC CCTGCTACTG TGTTTCCCCG AAAGAAGCTG 
TTCATCACAG CTGCCATCGT CGTTATATTC TTCACTGTCT TCATCCTGGG AATAACCATC 
CGGCCACTGG TGGAATTTCT TGATGTTAAG AGATCCAATA AGAAGCAGCA AGCTGTCAGT 
GAAGAGATCC ACTGTCGGTT TTTTGATCAT GTGAAGACTG GGATTGAAGA TGTTTGTGGA 
CACTGGGGTC ACAACTTCTG GAGAGACAAG TTTAAGAAAT TTGATGACAA ATACCTTCGG 
AAGCTTCTGA TTCGGGAAAA CCAACCCAAG TCTAGCATTG TGTCCTTATA TAAAAAGCTT 
GAAATAAAAC ATGCCATAGA GATGGCAGAG ACAGGGATGA TAAGCACTGT CCCCTCATTT 
GCATCTCTCA ACGACTGCCG TGAAGAGAAA ATAAGGAAGC TCACGCCAGG AGAAATGGAT 
GAAATTAGAG AGATATTGTC AAGGAATCTC TATCAAATCC GCCAGCGGAC CTTGTCCTAC 
AACAGACACA ACCTGACGGC AGACACAAGT GAGAGGCAAG CCAAGGAGAT CTTGATTCGC 
CGACGGCACA GTCTTCGGGA AAGTCTTAGG AAGGACAACA GCTTAAACCG CGAGCGCAGG 
GCTTCTACTT CAACCTCCCG ATATTTATCC TTACCTAAAA ACACAAAGCT TCCCGAGAAG 
TTGCAGAAGA AGAATAAGGT TTCAAATGCA GATGGTAATA GCAGCGACTC GGACATGGAT 
GGAACCACTG TACTCAATTT GCAGCCCAGG GCCAGGCGCT TCTTGCCAGA TCAGTTCTCA 
AAGAAAGCCT CACCAGCCTA CAAAATGGAA TGGAAGAATG AGGTGGACGT GGGTTCTGCG 
CGAGCGCCCC CCAGCGTCAC TCCAGCGCCT CGCAGTAAAG AGGGGGGCAC CCAGACACCA 
GGGGTGCTGA GGCAGCCCCT GCTCTCCAAA GACCAACGGT TTGGCCGGGG CAGGGAAGAC 
AGTTTGACTG AAGATGTTCC ACCCAAGCCC CCACCAAGGT TGGTGCGACG AGCATCAGAA 
CCCGGAAATA GGAAAGGCCG CCTTGGCAAC GAAAAGCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Na
Journal of cellular physiology232(7)1669-1680(2017 Jul)
Paehler Vor der Nolte A,Chodisetti G,Yuan Z,Busch F,Riederer B,Luo M,Yu Y,Menon MB,Schneider A,Stripecke R,Nikolovska K,Yeruva S,Seidler U

AQP2-Induced Acceleration of Renal Cell Proliferation Involves the Activation of a Regulatory Volume Increase Mechanism Dependent on NHE2.
Journal of cellular biochemistry118(5)967-978(2017 May)
Rivarola V,Di Giusto G,Christensen MJ,Ford P,Capurro C

Mechanism of down regulation of Na-H exchanger-2 in experimental colitis.
PloS one12(5)e0176767(2017)
Soleiman AA,Thameem F,Khan I

Na-H Exchanger Isoform-2 (NHE2) Mediates Butyrate-dependent Na+ Absorption in Dextran Sulfate Sodium (DSS)-induced Colitis.
The Journal of biological chemistry290(42)25487-96(2015 Oct)
Rajendran VM,Nanda Kumar NS,Tse CM,Binder HJ

Enhanced Expression of Sodium Hydrogen Exchanger (NHE)-1, 2 and 4 in the Cervix of Ovariectomised Rats by Phytoestrogen Genistein.
International journal of medical sciences12(6)468-77(2015)
Ismail N,Giribabu N,Muniandy S,Salleh N