Pard6a cDNA ORF clone, Rattus norvegicus(Norway rat)
| Gene Symbol | Pard6a |
| Entrez Gene ID | 307799 |
| Full Name | par-6 family cell polarity regulator alpha |
| Synonyms | Par-6a,Par6a |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Rattus norvegicus(Norway rat) |
| Genome | |
| Summary | The protein encoded by this gene is a component of the partitioning defective complex, a protein complex involved in controlling epithelial cell polarity as well as other cell processes. In rat testis cells, the protein localizes to the apical ectoplasmic specialization at the Sertoli-elongating spermatid interface and at the blood-testis barrier. Accordingly, knockdown of this gene in Sertoli cell epithelia results in compromised blood-testis barrier integrity. Knockdown of this gene in various neuronal cell types results in a variety of phenotypes including abnormal dendritic spine morphogenesis and defective axon outgrowth to the spinal midline. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]. |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_001003654.2 | NP_001003654.2 | partitioning defective 6 homolog alpha isoform 2 |
| NM_001003653.2 | NP_001003653.2 | partitioning defective 6 homolog alpha isoform 1 |
| XM_006255490.3 | XP_006255552.1 | partitioning defective 6 homolog alpha isoform X1 |
| Canis lupus familiaris (dog) | PARD6A | XP_546876.5 |
| Mus musculus (house mouse) | Pard6a | NP_062669.2 |
| Gallus gallus (chicken) | PARD6A | XP_004944104.1 |
| Danio rerio (zebrafish) | pard6a | NP_001093521.2 |
| PARD6A | XP_001092684.1 | |
| Bos taurus (cattle) | PARD6A | NP_001039739.1 |
| Homo sapiens (human) | PARD6A | NP_058644.1 |
| Pan troglodytes (chimpanzee) | PARD6A | XP_511037.2 |
| Rattus norvegicus (Norway rat) | Pard6a | NP_001003653.1 |
Related articles in PubMed
MicroRNA let-7f-5p regulates neuronal differentiation of rat bone marrow mesenchymal stem cells by targeting Par6?.
Han L, Wang Y, Wang L, Guo B, Pei S, Jia Y
Biochemical and biophysical research communications495(1)1476-1481(2018 01)
Suppression of CIP4/Par6 attenuates TGF-?1-induced epithelial-mesenchymal transition in NRK-52E cells.
Zhu YC, Wang YK, Bai SJ, Zha FF, Feng G, Gao CP, Liu J
International journal of molecular medicine40(4)1165-1171(2017 Oct)
FRMD4A-cytohesin signaling modulates the cellular release of tau.
Yan X, Nyk?nen NP, Brunello CA, Haapasalo A, Hiltunen M, Uronen RL, Huttunen HJ
Journal of cell science129(10)2003-15(2016 05)
NGL-2 Is a New Partner of PAR Complex in Axon Differentiation.
Xu G, Wang R, Wang Z, Lei Q, Yu Z, Liu C, Li P, Yang Z, Cheng X, Li G, Wu M
The Journal of neuroscience : the official journal of the Society for Neuroscience35(18)7153-64(2015 May)
Axonal elongation triggered by stimulus-induced local translation of a polarity complex protein.
Hengst U, Deglincerti A, Kim HJ, Jeon NL, Jaffrey SR
Nature cell biology11(8)1024-30(2009 Aug)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Altogether, our studies proved that inhibition of let-7f-5p facilitates induction of MSCs into neuron-like cells by directly targeting Par6alpha.
Title: MicroRNA let-7f-5p regulates neuronal differentiation of rat bone marrow mesenchymal stem cells by targeting Par6?.
NGL-2 binds to the PDZ domain of PAR6 which acts as a bridge or modifier in axon differentiation.
Title: NGL-2 Is a New Partner of PAR Complex in Axon Differentiation.
Results identify a new role for local translation and the PAR complex in axonal outgrowth.
Title: Axonal elongation triggered by stimulus-induced local translation of a polarity complex protein.
the Par6/Par3-based polarity complex likely coordinates the events of apical ectoplasmic specialization and blood-testis barrier restructuring that take place concurrently at the opposing ends of adjacent Sertoli cells during spermatogenesis
Title: Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis.
These results define a role for PAR-6 and aPKC in dendritic spine biogenesis and maintenance, and reveal an unexpected link between the PAR-6/aPKC complex and RhoA activity.
Title: The PAR-6 polarity protein regulates dendritic spine morphogenesis through p190 RhoGAP and the Rho GTPase.
The following Pard6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pard6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa46547 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001003653.2 | |
| Accession Version | NM_001003653.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-27 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | partitioning defective 6 homolog alpha isoform 1 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106288.5. On Mar 27, 2015 this sequence version replaced NM_001003653.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY682587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCCAGGC CGCAGAGGAC TCCGGCGCGC AGTCCGGATA GCATCGTCGA GGTGAAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001003653.2 |
| CDS | 36..1073 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001003653.2 |
ORF Insert Sequence:
1 | ATGGCCAGGC CGCAGAGGAC TCCGGCGCGC AGTCCGGATA GCATCGTCGA GGTGAAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa46548 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001003654.2 | |
| Accession Version | NM_001003654.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-20 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | partitioning defective 6 homolog alpha isoform 2 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106288.5. On Mar 27, 2015 this sequence version replaced NM_001003654.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY682586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMN01906349 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGTGTTC GTGTGGCTCC TCAGGGTCTG GAGCGGGTAC CTGGGATCTT CATCTCCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001003654.2 |
| CDS | 593..1105 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001003654.2 |
ORF Insert Sequence:
1 | ATGAGTGTTC GTGTGGCTCC TCAGGGTCTG GAGCGGGTAC CTGGGATCTT CATCTCCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa38852 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006255490.3 | |
| Accession Version | XM_006255490.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | partitioning defective 6 homolog alpha isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCCAGGC CGCAGAGGAC TCCGGCGCGC AGTCCGGATA GCATCGTCGA GGTGAAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006255552.1 |
| CDS | 109..1149 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006255490.3 |
ORF Insert Sequence:
1 | ATGGCCAGGC CGCAGAGGAC TCCGGCGCGC AGTCCGGATA GCATCGTCGA GGTGAAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
MicroRNA let-7f-5p regulates neuronal differentiation of rat bone marrow mesenchymal stem cells by targeting Par6?. |
|
Suppression of CIP4/Par6 attenuates TGF-?1-induced epithelial-mesenchymal transition in NRK-52E cells. |
|
FRMD4A-cytohesin signaling modulates the cellular release of tau. |
|
NGL-2 Is a New Partner of PAR Complex in Axon Differentiation. |
|
Axonal elongation triggered by stimulus-induced local translation of a polarity complex protein. |