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Melk cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Melk
Entrez Gene ID 362510
Full Name maternal embryonic leucine zipper kinase
General protein information
Preferred Names

maternal embryonic leucine zipper kinase

Names

maternal embryonic leucine zipper kinase
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

5

5q22

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001108662.1 NP_001102132.1 maternal embryonic leucine zipper kinase
XM_006238082.3 XP_006238144.1 maternal embryonic leucine zipper kinase isoform X1

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) MELK XP_538730.2
Gallus gallus (chicken) MELK NP_001026680.1
Xenopus tropicalis (tropical clawed frog) melk NP_001016390.1
Rattus norvegicus (Norway rat) Melk NP_001102132.1
Pan troglodytes (chimpanzee) MELK XP_001169038.1
Mus musculus (house mouse) Melk NP_034920.2
Danio rerio (zebrafish) melk NP_996771.2
Arabidopsis thaliana (thale cress) CIPK7 NP_188940.1
Homo sapiens (human) MELK NP_055606.1
MELK XP_001115076.2
Bos taurus (cattle) MELK NP_001104730.1
Caenorhabditis elegans (roundworm) pig-1 NP_001023420.2
Arabidopsis thaliana (thale cress) CIPK4 NP_193194.1

Gene Ontology


Bibliography

Related articles in PubMed

Kinase associated-1 domains drive MARK/PAR1 kinases to membrane targets by binding acidic phospholipids.
Moravcevic K, Mendrola JM, Schmitz KR, Wang YH, Slochower D, Janmey PA, Lemmon MA
Cell143(6)966-77(2010 Dec)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Murine protein serine/threonine kinase 38 activates apoptosis signal-regulating kinase 1 via Thr 838 phosphorylation.
Jung H, Seong HA, Ha H
The Journal of biological chemistry283(50)34541-53(2008 Dec)

Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family.
Lin ML, Park JH, Nishidate T, Nakamura Y, Katagiri T
Breast cancer research : BCR9(1)R17(2007)

Substrate specificity and activity regulation of protein kinase MELK.
Beullens M, Vancauwenbergh S, Morrice N, Derua R, Ceulemans H, Waelkens E, Bollen M
The Journal of biological chemistry280(48)40003-11(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Melk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Melk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa08064 NM_001108662.1
Latest version!		 Rattus norvegicus maternal embryonic leucine zipper kinase (Melk), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00
ORa28150 XM_006238082.3
Latest version!		 Rattus norvegicus maternal embryonic leucine zipper kinase (Melk), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa08064
Clone ID Related Accession (Same CDS sequence) NM_001108662.1
Accession Version NM_001108662.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1089bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-27
Organism Rattus norvegicus(Norway rat)
Product maternal embryonic leucine zipper kinase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473962.1. On or before Oct 4, 2007 this sequence version replaced XM_342828.3, XM_001064686.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2689604, SAMN01906347 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAAAGATT ATGACGAGCT CCTTAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGTCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AGAATGCACT AGGGAGCGAT TTGCCCCGAG TCAAAACTGA AATTGATGCC 
CTGAAGAATC TGAGACATCA GCATATATGT CAGCTCTACC ATGTGCTAGA GACAAAGAAC 
AAAATATTCA TGGTGCTTGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATCATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGA GTCATCTTCC GCCAGATCCT GTCTGCAGTT 
GCATATGTGC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AGCCAGAAAA TTTATTGTTT 
GACGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTACAGAC ATGCTGTGGG AGTCTGGCGT ATGCAGCACC TGAACTAATA 
CAAGGGAAAT CGTATCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTATAT 
GTGCTTATGT GTGGATTCCT CCCGTTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGAGAG GGAAGTATGA AGTTCCCAAG TGGCTGTCTC CCAGCAGCAT CCTGCTTCTC 
CAGCAGATGC TTCAGGTGGA CCCAAAGAGA CGGATTTCTA TGAAAAATCT CTTGAACCAT 
CCCTGGATCC TGCAAGATTA CAGCTGTCCC ATTGAGTGGC AAAGCAAGGC TTCTTTGACT 
CACCTTGATG AGGATTGCAT AACAGAGCTT TCTGTACATC ACCGCAACAG CAGGCAAACA 
ATGGAGGATT TAATTTCGTC GTGGCAGTAT GATCACCTCA CAGCCACCTA CCTTCTGCTT 
CTAGCCAAGA AGGCTCGAGG GAAGCCAGCT CGTCTGCAGC TTCTGTCCTT CTCTGGTGGG 
ACCACCAGCA CCACTCCAAA GGCAATCAAA GAACCTGAGC CTGGAAGATA TGTCCACAAG 
TGGCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001102132.1
CDS126..1214
Translation

Target ORF information:

RefSeq Version NM_001108662.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus maternal embryonic leucine zipper kinase (Melk), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001108662.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAAAGATT ATGACGAGCT CCTTAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGTCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AGAATGCACT AGGGAGCGAT TTGCCCCGAG TCAAAACTGA AATTGATGCC 
CTGAAGAATC TGAGACATCA GCATATATGT CAGCTCTACC ATGTGCTAGA GACAAAGAAC 
AAAATATTCA TGGTGCTTGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATCATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGA GTCATCTTCC GCCAGATCCT GTCTGCAGTT 
GCATATGTGC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AGCCAGAAAA TTTATTGTTT 
GACGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTACAGAC ATGCTGTGGG AGTCTGGCGT ATGCAGCACC TGAACTAATA 
CAAGGGAAAT CGTATCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTATAT 
GTGCTTATGT GTGGATTCCT CCCGTTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGAGAG GGAAGTATGA AGTTCCCAAG TGGCTGTCTC CCAGCAGCAT CCTGCTTCTC 
CAGCAGATGC TTCAGGTGGA CCCAAAGAGA CGGATTTCTA TGAAAAATCT CTTGAACCAT 
CCCTGGATCC TGCAAGATTA CAGCTGTCCC ATTGAGTGGC AAAGCAAGGC TTCTTTGACT 
CACCTTGATG AGGATTGCAT AACAGAGCTT TCTGTACATC ACCGCAACAG CAGGCAAACA 
ATGGAGGATT TAATTTCGTC GTGGCAGTAT GATCACCTCA CAGCCACCTA CCTTCTGCTT 
CTAGCCAAGA AGGCTCGAGG GAAGCCAGCT CGTCTGCAGC TTCTGTCCTT CTCTGGTGGG 
ACCACCAGCA CCACTCCAAA GGCAATCAAA GAACCTGAGC CTGGAAGATA TGTCCACAAG 
TGGCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa28150
Clone ID Related Accession (Same CDS sequence) XM_006238082.3
Accession Version XM_006238082.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product maternal embryonic leucine zipper kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGACGAGCT CCTTAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGTCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AGAATGCACT AGGGAGCGAT TTGCCCCGAG TCAAAACTGA AATTGATGCC 
CTGAAGAATC TGAGACATCA GCATATATGT CAGCTCTACC ATGTGCTAGA GACAAAGAAC 
AAAATATTCA TGGTGCTTGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATCATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGA GTCATCTTCC GCCAGATCCT GTCTGCAGTT 
GCATATGTGC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AGCCAGAAAA TTTATTGTTT 
GACGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTACAGAC ATGCTGTGGG AGTCTGGCGT ATGCAGCACC TGAACTAATA 
CAAGGGAAAT CGTATCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTATAT 
GTGCTTATGT GTGGATTCCT CCCGTTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGAGAG GGAAGTATGA AGTTCCCAAG TGGCTGTCTC CCAGCAGCAT CCTGCTTCTC 
CAGCAGATGC TTCAGGTGGA CCCAAAGAGA CGGATTTCTA TGAAAAATCT CTTGAACCAT 
CCCTGGATCC TGCAAGATTA CAGCTGTCCC ATTGAGTGGC AAAGCAAGGC TTCTTTGACT 
CACCTTGATG AGGATTGCAT AACAGAGCTT TCTGTACATC ACCGCAACAG CAGGCAAACA 
ATGGAGGATT TAATTTCGTC GTGGCAGTAT GATCACCTCA CAGCCACCTA CCTTCTGCTT 
CTAGCCAAGA AGGCTCGAGG GAAGCCAGCT CGTCTGCAGC TTCTGTCCTT CTCTGGTGGG 
ACCACCAGCA CCACTCCAAA GTCAAAGAAC CTGAGCCTGG AAGATATGTC CACAAGTGGC 
GATAGCTGTG TGGCTGGATT AATAGACTAT GAATTGTGTG AAGATAATTT ACTAGCTCCC 
CAGACACCAC AGTTTACCAA ACACTGGGCA GAATCAAATC ATGTTGAATG TGAATCACCA 
ACTCCAGTGG TATGCAGAGC ACTGGCCAAT AAGTTAAAGG ACAAAGAGAA TGTGTGCACT 
CCCAAGTCTT CTGTGAAGAA TCAAGAGCAA TTCGTGTTTT CTGAGCCGAA GATTCCAGTT 
AGTCGGAACC AGTATAAGAG AGAAATACTC ACCTCACCAA CCCAGTTCAC AGCACCTGCA 
AAAGCTAGAA CGCAGTGCCT GAGAGAAACC CAGGTTAGAA CGCCGGTGAA TTCCGCAGGA 
GCAGACACGC TAACGACAAA TGTCATTAGC CCTGAGAGGA GGTGCCGTTC AATGGAAGTG 
GATCTCAACC AGGCACACAT GGAGGATACC CCTAAAAGGA AAGGAACCAA TGTGTTTGGG 
AGCCTTGAGA GAGGACTGGA TAAGGTTCTC ACTGCACTTA CAAGGAACAA GAAGAAGGGC 
TCTGCCAAAG ATGGGCCAAG AAGGCGAAAG CTGCACTACA ATGTGACTAC AACTAGACTG 
GTGAATCCCG ACCAGCTCCT GAACGAAATC ATGGCTATTC TTCCAAAGAA GAACGTGGAC 
TTCGTGCAGA AAGGTTACAC ACTGAAGTGT CAGACACAGT CTGATTTTGG CAAAGTGACA 
ATGCAGTTTG AACTGGAAGT GTGCCAGCTG CAGAGACCTG ACGTGGTGGG TATCCGGAGA 
CAGCGGCTGA AGGGTGATGC CTGGGTTTAC AAGAGATTAG TGGAAGATAT CTTGTCTGGC 
TGCAAGATGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006238144.1
CDS181..2112
Translation

Target ORF information:

RefSeq Version XM_006238082.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus maternal embryonic leucine zipper kinase (Melk), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006238082.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGACGAGCT CCTTAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGTCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AGAATGCACT AGGGAGCGAT TTGCCCCGAG TCAAAACTGA AATTGATGCC 
CTGAAGAATC TGAGACATCA GCATATATGT CAGCTCTACC ATGTGCTAGA GACAAAGAAC 
AAAATATTCA TGGTGCTTGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATCATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGA GTCATCTTCC GCCAGATCCT GTCTGCAGTT 
GCATATGTGC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AGCCAGAAAA TTTATTGTTT 
GACGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTACAGAC ATGCTGTGGG AGTCTGGCGT ATGCAGCACC TGAACTAATA 
CAAGGGAAAT CGTATCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTATAT 
GTGCTTATGT GTGGATTCCT CCCGTTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGAGAG GGAAGTATGA AGTTCCCAAG TGGCTGTCTC CCAGCAGCAT CCTGCTTCTC 
CAGCAGATGC TTCAGGTGGA CCCAAAGAGA CGGATTTCTA TGAAAAATCT CTTGAACCAT 
CCCTGGATCC TGCAAGATTA CAGCTGTCCC ATTGAGTGGC AAAGCAAGGC TTCTTTGACT 
CACCTTGATG AGGATTGCAT AACAGAGCTT TCTGTACATC ACCGCAACAG CAGGCAAACA 
ATGGAGGATT TAATTTCGTC GTGGCAGTAT GATCACCTCA CAGCCACCTA CCTTCTGCTT 
CTAGCCAAGA AGGCTCGAGG GAAGCCAGCT CGTCTGCAGC TTCTGTCCTT CTCTGGTGGG 
ACCACCAGCA CCACTCCAAA GTCAAAGAAC CTGAGCCTGG AAGATATGTC CACAAGTGGC 
GATAGCTGTG TGGCTGGATT AATAGACTAT GAATTGTGTG AAGATAATTT ACTAGCTCCC 
CAGACACCAC AGTTTACCAA ACACTGGGCA GAATCAAATC ATGTTGAATG TGAATCACCA 
ACTCCAGTGG TATGCAGAGC ACTGGCCAAT AAGTTAAAGG ACAAAGAGAA TGTGTGCACT 
CCCAAGTCTT CTGTGAAGAA TCAAGAGCAA TTCGTGTTTT CTGAGCCGAA GATTCCAGTT 
AGTCGGAACC AGTATAAGAG AGAAATACTC ACCTCACCAA CCCAGTTCAC AGCACCTGCA 
AAAGCTAGAA CGCAGTGCCT GAGAGAAACC CAGGTTAGAA CGCCGGTGAA TTCCGCAGGA 
GCAGACACGC TAACGACAAA TGTCATTAGC CCTGAGAGGA GGTGCCGTTC AATGGAAGTG 
GATCTCAACC AGGCACACAT GGAGGATACC CCTAAAAGGA AAGGAACCAA TGTGTTTGGG 
AGCCTTGAGA GAGGACTGGA TAAGGTTCTC ACTGCACTTA CAAGGAACAA GAAGAAGGGC 
TCTGCCAAAG ATGGGCCAAG AAGGCGAAAG CTGCACTACA ATGTGACTAC AACTAGACTG 
GTGAATCCCG ACCAGCTCCT GAACGAAATC ATGGCTATTC TTCCAAAGAA GAACGTGGAC 
TTCGTGCAGA AAGGTTACAC ACTGAAGTGT CAGACACAGT CTGATTTTGG CAAAGTGACA 
ATGCAGTTTG AACTGGAAGT GTGCCAGCTG CAGAGACCTG ACGTGGTGGG TATCCGGAGA 
CAGCGGCTGA AGGGTGATGC CTGGGTTTAC AAGAGATTAG TGGAAGATAT CTTGTCTGGC 
TGCAAGATGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Kinase associated-1 domains drive MARK/PAR1 kinases to membrane targets by binding acidic phospholipids.
Cell143(6)966-77(2010 Dec)
Moravcevic K,Mendrola JM,Schmitz KR,Wang YH,Slochower D,Janmey PA,Lemmon MA


book

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA


book

Murine protein serine/threonine kinase 38 activates apoptosis signal-regulating kinase 1 via Thr 838 phosphorylation.
The Journal of biological chemistry283(50)34541-53(2008 Dec)
Jung H,Seong HA,Ha H


book

Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family.
Breast cancer research : BCR9(1)R17(2007)
Lin ML,Park JH,Nishidate T,Nakamura Y,Katagiri T


book

Substrate specificity and activity regulation of protein kinase MELK.
The Journal of biological chemistry280(48)40003-11(2005 Dec)
Beullens M,Vancauwenbergh S,Morrice N,Derua R,Ceulemans H,Waelkens E,Bollen M