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melk cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol melk
Entrez Gene ID 30724
Full Name maternal embryonic leucine zipper kinase
Synonyms fb71b01,fe01f04,wu:fb71b01,wu:fe01f04
General protein information
Preferred Names

maternal embryonic leucine zipper kinase

Names

maternal embryonic leucine zipper kinase
protein kinase PK38
zMelk
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_206888.2 NP_996771.2 maternal embryonic leucine zipper kinase

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) MELK XP_538730.2
Gallus gallus (chicken) MELK NP_001026680.1
Xenopus tropicalis (tropical clawed frog) melk NP_001016390.1
Rattus norvegicus (Norway rat) Melk NP_001102132.1
Pan troglodytes (chimpanzee) MELK XP_001169038.1
Mus musculus (house mouse) Melk NP_034920.2
Danio rerio (zebrafish) melk NP_996771.2
Arabidopsis thaliana (thale cress) CIPK7 NP_188940.1
Homo sapiens (human) MELK NP_055606.1
MELK XP_001115076.2
Bos taurus (cattle) MELK NP_001104730.1
Caenorhabditis elegans (roundworm) pig-1 NP_001023420.2
Arabidopsis thaliana (thale cress) CIPK4 NP_193194.1

Gene Ontology


Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
Deng C, Chen H, Yang N, Feng Y, Hsueh AJ
The Journal of biological chemistry290(30)18261-8(2015 Jul)

Normalization of RNA-sequencing data from samples with varying mRNA levels.
Aanes H, Winata C, Moen LF, ?strup O, Mathavan S, Collas P, Rognes T, Alestr?m P
PloS one9(2)e89158(2014)

Toxicological assessment of trace ?-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
Yin X, Wang H, Zhang Y, Dahlgren RA, Zhang H, Shi M, Gao M, Wang X
PloS one9(7)e102731(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following melk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the melk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa37363 NM_206888.2
Latest version!		 Danio rerio maternal embryonic leucine zipper kinase (melk), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa37363
Clone ID Related Accession (Same CDS sequence) NM_206888.2
Accession Version NM_206888.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-15
Organism Danio rerio(zebrafish)
Product maternal embryonic leucine zipper kinase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC050520.1. On Apr 5, 2004 this sequence version replaced NM_206888.1. ##Evidence-Data-START## Transcript exon combination :: BC050520.1, AB108827.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

1
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721
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841
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961
1021
1081
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1441
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1561
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1981
ATGCCAGTAG ACAGCACTTC TGAACTCCTC AGGCACTATG AGGTTTATGA AACCATTGGC 
TCAGGTGGGT TTGCCAAGGT CAAACTTGGC AGACACAAAC TGACAGGAGA GAAAGTAGCC 
ATCAAAATAA TGGAGAAAAA AGATCTTGGG GATGATCTGC CCAGAGTAAA GATAGAGATT 
GAGGCAATGA AGAACCTCAG TCATCAACAT GTATGTCGTC TCTACCATGT CATTGAGACC 
ACAAGCAAAA TCTACATGGT GCTTGAGTAC TGTCCTGGAG GAGAACTCTT TGATTACATC 
ATTGCTAAAG ATCGGTTATC AGAGGAAGAG ACCCGAGTGT TTTTTCGCCA GATCATCTCT 
GCGTTGGCCT ATGTTCATAG CCAAGGTTAT GCCCATCGAG ACCTCAAACC TGAAAACTTG 
CTGATCGATG AAGATCATAA CCTAAAACTC ATTGATTTTG GCCTGTGTGC CAAGCCAAAG 
GGTGGTTTGG GTTTTGAATT GCTGACATGT TGTGGCAGTC CTGCATACGC AGCTCCAGAG 
CTCATTCAGG GCAAGGCTTA CATCGGCTCT GAGGCTGATG TTTGGAGTAT GGGTGTATTG 
CTTTATGCAC TACTGTGTGG TTTTCTACCA TTTGATGATG ACAATTGTAT GGTTCTCTAT 
AGAAAAATCA CAAGAGGCAA ATACAGCAAC CCACACTGGC TTTCACCTTC TAGTATTCTG 
CTTCTAAACC AAATGATGCA GGTGGACCCA AAGAGGCGTT TGACAGTGAA ACATTTGCTT 
GATCACCCAT GGGCTATGAG GGGCTACAGC ACACCAGTGG AGTGGCACAG TAAATATCCA 
TTGGGTCATA TAGATGAAGA CTGCATCACA GAAATGGCGG TGACTTTCAA ACAGTCCAAA 
CAACGAACTA TCCAGCTTGT GTCTGAGTGG AAGTATGATC AAATAACAGC AACCTACCTT 
TTACTGCTGG CTAAGAAGCG TCAAGGCCGT CCTGTGCGTC TGAGAGCTGA ATGCCCTGTG 
ATTGACATTG TGTGTTCTCC TTTACAGGAT ATGCAACTGA AGAAGAGTCT ACGCTTCACT 
GAGGATGATG ATGGTGTTCA CCCAGTGGTG CTCGGCTCTA TGGTCTTTCC CCCTGATTGC 
TACGATGACG AGAATCCCTG GACCCCTCTC ACACCCAAGA ACACACATAC CACAAACACT 
CCACGCATGA AGCTGTACCC TGAAACAACA GAGAAGTGGA ATGAAATGGC TTACTCCCCC 
GTCATAGAGC ACAGCAGACC CTGCCGACAG AAGCCAGAGA GACGTGACAG AACCAAGGAA 
AATAAAGAGA ACCTGGCAGT GCCAGGCACA GATGGAGATG TTTTTGCTCT GCCAGCGCCG 
CGAACTCCAA CATCCAGCCG AAAGGTCAAA TCCAACAGAA CTGTAATGAC AACGCCCAAC 
CACAACAACA ACAAGAGCAG CGAAGTAAAC AAAGGAGCGG GCAGTGCCAC TAAAGAAGGG 
TCCCGCAGGA GAGAGGTGGA ACAGCAGCAG AACGGCCAAC AAGGAGAACT CAATATGTTG 
GCTTTTAGCC CTGAGCGAAG GTCTCGATCA CTGGACCTGG CAGGCTGTCA GGTGGACAGT 
GGGCAGAAGC GTAAGGGAGG GAAAGTGTTT GGCAGTCTGG AAAGAGGCCT GGATAAAGTC 
ATTACAATGC TTACACCCAG TAAAAAGAGA GGACCTCGAG ATGGCCCACG CAAAATTAAG 
GCTCAGTATA ATGTAACTCT CACCAATCAA ACGAATGCTG ACCAAGTGCT CAATCAGATT 
CTCAGCATAT TACCAGAGAA GAATGTGGAC TTTGTTCAGA AGGGCTATAC TCTGAAATGC 
CACACGCAGT CAGACTTTGG GAAGGTCACC ATGCAGTTCG AGCTGGAGGT TTGTCTCCTG 
CAGAAGCCTG AGGTTGTGGG GATCCGTCGA CAACGGCTCA AAGGAGATGC CTGGGTGTAT 
AAACACCCGG TAGAGGACAT TCTGTCCTCC TCCAGCCAAT GGTCTGCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_996771.2
CDS224..2254
Translation

Target ORF information:

RefSeq Version NM_206888.2
Organism Danio rerio(zebrafish)
Definition Danio rerio maternal embryonic leucine zipper kinase (melk), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_206888.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCCAGTAG ACAGCACTTC TGAACTCCTC AGGCACTATG AGGTTTATGA AACCATTGGC 
TCAGGTGGGT TTGCCAAGGT CAAACTTGGC AGACACAAAC TGACAGGAGA GAAAGTAGCC 
ATCAAAATAA TGGAGAAAAA AGATCTTGGG GATGATCTGC CCAGAGTAAA GATAGAGATT 
GAGGCAATGA AGAACCTCAG TCATCAACAT GTATGTCGTC TCTACCATGT CATTGAGACC 
ACAAGCAAAA TCTACATGGT GCTTGAGTAC TGTCCTGGAG GAGAACTCTT TGATTACATC 
ATTGCTAAAG ATCGGTTATC AGAGGAAGAG ACCCGAGTGT TTTTTCGCCA GATCATCTCT 
GCGTTGGCCT ATGTTCATAG CCAAGGTTAT GCCCATCGAG ACCTCAAACC TGAAAACTTG 
CTGATCGATG AAGATCATAA CCTAAAACTC ATTGATTTTG GCCTGTGTGC CAAGCCAAAG 
GGTGGTTTGG GTTTTGAATT GCTGACATGT TGTGGCAGTC CTGCATACGC AGCTCCAGAG 
CTCATTCAGG GCAAGGCTTA CATCGGCTCT GAGGCTGATG TTTGGAGTAT GGGTGTATTG 
CTTTATGCAC TACTGTGTGG TTTTCTACCA TTTGATGATG ACAATTGTAT GGTTCTCTAT 
AGAAAAATCA CAAGAGGCAA ATACAGCAAC CCACACTGGC TTTCACCTTC TAGTATTCTG 
CTTCTAAACC AAATGATGCA GGTGGACCCA AAGAGGCGTT TGACAGTGAA ACATTTGCTT 
GATCACCCAT GGGCTATGAG GGGCTACAGC ACACCAGTGG AGTGGCACAG TAAATATCCA 
TTGGGTCATA TAGATGAAGA CTGCATCACA GAAATGGCGG TGACTTTCAA ACAGTCCAAA 
CAACGAACTA TCCAGCTTGT GTCTGAGTGG AAGTATGATC AAATAACAGC AACCTACCTT 
TTACTGCTGG CTAAGAAGCG TCAAGGCCGT CCTGTGCGTC TGAGAGCTGA ATGCCCTGTG 
ATTGACATTG TGTGTTCTCC TTTACAGGAT ATGCAACTGA AGAAGAGTCT ACGCTTCACT 
GAGGATGATG ATGGTGTTCA CCCAGTGGTG CTCGGCTCTA TGGTCTTTCC CCCTGATTGC 
TACGATGACG AGAATCCCTG GACCCCTCTC ACACCCAAGA ACACACATAC CACAAACACT 
CCACGCATGA AGCTGTACCC TGAAACAACA GAGAAGTGGA ATGAAATGGC TTACTCCCCC 
GTCATAGAGC ACAGCAGACC CTGCCGACAG AAGCCAGAGA GACGTGACAG AACCAAGGAA 
AATAAAGAGA ACCTGGCAGT GCCAGGCACA GATGGAGATG TTTTTGCTCT GCCAGCGCCG 
CGAACTCCAA CATCCAGCCG AAAGGTCAAA TCCAACAGAA CTGTAATGAC AACGCCCAAC 
CACAACAACA ACAAGAGCAG CGAAGTAAAC AAAGGAGCGG GCAGTGCCAC TAAAGAAGGG 
TCCCGCAGGA GAGAGGTGGA ACAGCAGCAG AACGGCCAAC AAGGAGAACT CAATATGTTG 
GCTTTTAGCC CTGAGCGAAG GTCTCGATCA CTGGACCTGG CAGGCTGTCA GGTGGACAGT 
GGGCAGAAGC GTAAGGGAGG GAAAGTGTTT GGCAGTCTGG AAAGAGGCCT GGATAAAGTC 
ATTACAATGC TTACACCCAG TAAAAAGAGA GGACCTCGAG ATGGCCCACG CAAAATTAAG 
GCTCAGTATA ATGTAACTCT CACCAATCAA ACGAATGCTG ACCAAGTGCT CAATCAGATT 
CTCAGCATAT TACCAGAGAA GAATGTGGAC TTTGTTCAGA AGGGCTATAC TCTGAAATGC 
CACACGCAGT CAGACTTTGG GAAGGTCACC ATGCAGTTCG AGCTGGAGGT TTGTCTCCTG 
CAGAAGCCTG AGGTTGTGGG GATCCGTCGA CAACGGCTCA AAGGAGATGC CTGGGTGTAT 
AAACACCCGG TAGAGGACAT TCTGTCCTCC TCCAGCCAAT GGTCTGCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J


book

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R


book

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
The Journal of biological chemistry290(30)18261-8(2015 Jul)
Deng C,Chen H,Yang N,Feng Y,Hsueh AJ


book

Normalization of RNA-sequencing data from samples with varying mRNA levels.
PloS one9(2)e89158(2014)
Aanes H,Winata C,Moen LF,?strup O,Mathavan S,Collas P,Rognes T,Alestr?m P


book

Toxicological assessment of trace ?-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
PloS one9(7)e102731(2014)
Yin X,Wang H,Zhang Y,Dahlgren RA,Zhang H,Shi M,Gao M,Wang X