Usf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usf2
Entrez Gene ID	81817
Full Name	upstream transcription factor 2, c-fos interacting
General protein information	Preferred Names upstream transcription factor 2, c-fos interacting Names upstream stimulatory factor 2 major late transcription factor 2 transcription factor USF2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	ubiquitous transcription factor; may play a role in hormonal and dietary regulations [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031139.1	NP_112401.1	upstream stimulatory factor 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	USF2	XP_855488.3
Mus musculus (house mouse)	Usf2	NP_035810.1
Danio rerio (zebrafish)	usf2	NP_001116257.1
Xenopus tropicalis (tropical clawed frog)	usf2	NP_001007857.1
	LOC718527	XP_001111345.2
Bos taurus (cattle)	USF2	NP_001001162.1
Rattus norvegicus (Norway rat)	Usf2	NP_112401.1
Homo sapiens (human)	USF2	NP_003358.1

Gene Ontology
Bibliography

Related articles in PubMed

Upstream stimulatory factor induces Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation.
Wood MA, Mukherjee P, Toocheck CA, Walker WH
Biology of reproduction85(5)965-76(2011 Nov)

Glycated albumin upregulates upstream stimulatory factor 2 gene transcription in mesangial cells.
Li Y, Wang S
American journal of physiology. Renal physiology299(1)F121-7(2010 Jul)

Overexpression of USF increases TGF-beta1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells.
Kim KS, Jung HS, Chung YJ, Jung TS, Jang HW, Lee MS, Kim KW, Chung JH
Journal of Korean medical science23(5)870-6(2008 Oct)

High glucose levels upregulate upstream stimulatory factor 2 gene transcription in mesangial cells.
Shi L, Liu S, Nikolic D, Wang S
Journal of cellular biochemistry103(6)1952-61(2008 Apr)

Control of the calcitonin gene-related peptide enhancer by upstream stimulatory factor in trigeminal ganglion neurons.
Park KY, Russo AF
The Journal of biological chemistry283(9)5441-51(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Upstream stimulatory factors (USF1 and USF2) induce Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation.
Title: Upstream stimulatory factor induces Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation.

Data suggest that glycated albumin upregulated USF2 gene transcription in mesangial cells through NF-kappaB-dependent transactivation of the USF2 promoter.
Title: Glycated albumin upregulates upstream stimulatory factor 2 gene transcription in mesangial cells.

USF has a role in increasing TGF-beta1 protein levels, but not in indusing G1 phase arrest
Title: Overexpression of USF increases TGF-beta1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells.

high glucose levels up-regulate USF2 gene transcription in mesangial cells through CREB-dependent transactivation of the USF2 promoter.
Title: High glucose levels upregulate upstream stimulatory factor 2 gene transcription in mesangial cells.

USF1 and USF2 are important contributors to neuronal-specific and mitogen-activated protein kinase regulation of the CGRP gene in trigeminal ganglion neurons
Title: Control of the calcitonin gene-related peptide enhancer by upstream stimulatory factor in trigeminal ganglion neurons.

The following Usf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07725	NM_031139.1 Latest version!	Rattus norvegicus upstream transcription factor 2, c-fos interacting (Usf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usf2 ( NM_031139.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112401.1 Rattus norvegicus upstream transcription factor 2, c-fos interacting (Usf2), mRNA.

Price & Availability↑

CloneID	ORa07725
Clone ID Related Accession (Same CDS sequence)	NM_031139.1
Accession Version	NM_031139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Rattus norvegicus(Norway rat)
Product	upstream stimulatory factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB047556.1. On Jun 25, 2006 this sequence version replaced XM_001079341.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB047556.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296 [ECO:0000350] ##Evidence-Data-END##

ATGGACATGC TGGACCCGGG TCTGGATCCC GCTTCCTCGG CCACCGCTGC TGCCGCCGCC 
AGCCACGACA AGGGACCCGA GGCCGAGGAG GGCGTTGAGC TGCAGGAAGG CGGGGACGGC 
CCGGGGGCTG AGGAGCAGAC GGCGGTGGCC ATCGCCAGCG TCCAGCAGGC GGCGTTCGGC 
GACCATAATA TCCAGTACCA GTTCCGCACA GAGAGTAATG GAGGCCAGGT GACATACCGC 
GTAGTCCAGG TGACTGATGG TCAGCTGGAC GGCCAGGGCG ACGCGGCTGG CGCCGTCAGC 
GTCGTGTCCA CCGCTGCCTT CGCAGGGGGG CAGCAGGCTG TGACCCAGGT GGGTGTGGAC 
GGGGCAGCCC AGCGCCCCGG CCCTGCCGCT GCCTCTGTAC CCACAGGTCC CGCAGCACCC 
TTCCCGCTGG CTGTAATTCA AAATCCATTC AGCAATGGTG GCAGCCCAGC AGCCGAAGCT 
GTCAGCGGGG AGGCACGGTT TGCTTATTTT CCAGCATCCA GTGTCGGCGA TACCACAGCT 
GTGTCTGTTC AAACTACAGA CCAGAGCCTA CAGGCCGGAG GCCAGTTCTA TGTCATGATG 
ACACCCCAGG ATGTGCTTCA AACAGGAACA CAGAGGACAA TTGCACCCCG GACACACCCC 
TATTCTCCGA AAATTGATGG AACCAGAACT CCTCGAGATG AGAGAAGAAG AGCTCAGCAC 
AATGAAGTGG AGCGGAGAAG GAGGGACAAG ATCAACAACT GGATTGTCCA GCTTTCAAAA 
ATCATTCCAG ATTGTCATGC AGACAACAGC AAGACAGGGG CGAGTAAAGG GGGCATCCTG 
TCCAAGGCTT GCGATTACAT CCGGGAGCTA CGCCAGACCA ACCAGCGTAT GCAGGAGACC 
TTCAAGGAGG CAGAGCGGCT GCAAATGGAC AATGAGCTCC TGAGGCAGCA GATCGAGGAG 
CTGAAGAACG AGAACGCCCT GCTTCGAGCC CAACTGCAGC AGCACAACCT GGAAATGGTG 
GGCGAGAGCA CCCGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112401.1
CDS	512..1552
Translation	MDMLDPGLDPASSATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAIASVQQAAFGDHNIQYQFRTESNGGQVTYRVVQVTDGQLDGQGDAAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPTGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ

Target ORF information:

RefSeq Version	NM_031139.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus upstream transcription factor 2, c-fos interacting (Usf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031139.1

ORF Insert Sequence:

ATGGACATGC TGGACCCGGG TCTGGATCCC GCTTCCTCGG CCACCGCTGC TGCCGCCGCC 
AGCCACGACA AGGGACCCGA GGCCGAGGAG GGCGTTGAGC TGCAGGAAGG CGGGGACGGC 
CCGGGGGCTG AGGAGCAGAC GGCGGTGGCC ATCGCCAGCG TCCAGCAGGC GGCGTTCGGC 
GACCATAATA TCCAGTACCA GTTCCGCACA GAGAGTAATG GAGGCCAGGT GACATACCGC 
GTAGTCCAGG TGACTGATGG TCAGCTGGAC GGCCAGGGCG ACGCGGCTGG CGCCGTCAGC 
GTCGTGTCCA CCGCTGCCTT CGCAGGGGGG CAGCAGGCTG TGACCCAGGT GGGTGTGGAC 
GGGGCAGCCC AGCGCCCCGG CCCTGCCGCT GCCTCTGTAC CCACAGGTCC CGCAGCACCC 
TTCCCGCTGG CTGTAATTCA AAATCCATTC AGCAATGGTG GCAGCCCAGC AGCCGAAGCT 
GTCAGCGGGG AGGCACGGTT TGCTTATTTT CCAGCATCCA GTGTCGGCGA TACCACAGCT 
GTGTCTGTTC AAACTACAGA CCAGAGCCTA CAGGCCGGAG GCCAGTTCTA TGTCATGATG 
ACACCCCAGG ATGTGCTTCA AACAGGAACA CAGAGGACAA TTGCACCCCG GACACACCCC 
TATTCTCCGA AAATTGATGG AACCAGAACT CCTCGAGATG AGAGAAGAAG AGCTCAGCAC 
AATGAAGTGG AGCGGAGAAG GAGGGACAAG ATCAACAACT GGATTGTCCA GCTTTCAAAA 
ATCATTCCAG ATTGTCATGC AGACAACAGC AAGACAGGGG CGAGTAAAGG GGGCATCCTG 
TCCAAGGCTT GCGATTACAT CCGGGAGCTA CGCCAGACCA ACCAGCGTAT GCAGGAGACC 
TTCAAGGAGG CAGAGCGGCT GCAAATGGAC AATGAGCTCC TGAGGCAGCA GATCGAGGAG 
CTGAAGAACG AGAACGCCCT GCTTCGAGCC CAACTGCAGC AGCACAACCT GGAAATGGTG 
GGCGAGAGCA CCCGGCAGTG A