Usf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Usf2
Entrez Gene ID	22282
Full Name	upstream transcription factor 2
Synonyms	Usf-2,bHLHb12
General protein information	Preferred Names upstream transcription factor 2 Names upstream stimulatory factor 2 major late transcription factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B1\|7 19.27 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006539734.3	XP_006539797.1	upstream stimulatory factor 2 isoform X2
XM_006539735.3	XP_006539798.1	upstream stimulatory factor 2 isoform X2
XM_006539733.3	XP_006539796.1	upstream stimulatory factor 2 isoform X1
XM_006539733.4	XP_006539796.1	upstream stimulatory factor 2 isoform X1
NM_011680.2	NP_035810.1	upstream stimulatory factor 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	USF2	XP_855488.3
Mus musculus (house mouse)	Usf2	NP_035810.1
Danio rerio (zebrafish)	usf2	NP_001116257.1
Xenopus tropicalis (tropical clawed frog)	usf2	NP_001007857.1
	LOC718527	XP_001111345.2
Bos taurus (cattle)	USF2	NP_001001162.1
Rattus norvegicus (Norway rat)	Usf2	NP_112401.1
Homo sapiens (human)	USF2	NP_003358.1

Gene Ontology
Bibliography

Related articles in PubMed

Pdx1 and USF transcription factors co-ordinately regulate Alx3 gene expression in pancreatic ?-cells.
Fern?ndez-P?rez A, Vallejo M
The Biochemical journal463(2)287-96(2014 Oct)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells.
Zeng L, Sun Y, Xie L, Wei L, Ren Y, Zhao J, Qin W, Mitchelson K, Cheng J
Proteomics13(16)2377-85(2013 Aug)

Upstream stimulatory factors 1 and 2 mediate the transcription of angiotensin II binding and inhibitory protein.
Matsuda M, Tamura K, Wakui H, Maeda A, Ohsawa M, Kanaoka T, Azushima K, Uneda K, Haku S, Tsurumi-Ikeya Y, Toya Y, Maeshima Y, Yamashita A, Umemura S
The Journal of biological chemistry288(26)19238-49(2013 Jun)

Differential configurations involving binding of USF transcription factors and Twist1 regulate Alx3 promoter activity in mesenchymal and pancreatic cells.
Garc?a-Sanz P, Fern?ndez-P?rez A, Vallejo M
The Biochemical journal450(1)199-208(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pdx1, USF1 and USF2 co-ordinately regulate Alx3 gene expression in pancreatic beta-cells.
Title: Pdx1 and USF transcription factors co-ordinately regulate Alx3 gene expression in pancreatic ?-cells.

AGTRAP expression is regulated by USF1 and USF2
Title: Upstream stimulatory factors 1 and 2 mediate the transcription of angiotensin II binding and inhibitory protein.

USF1 and USF2 are important regulatory factors for Alx3 gene expression in different cell types
Title: Differential configurations involving binding of USF transcription factors and Twist1 regulate Alx3 promoter activity in mesenchymal and pancreatic cells.

Data suggest that both USF1 and USF2 are essential for angiotensinogen (AGT) transcriptional regulation, and distinct sex-specific and tissue-specific mechanisms are involved in the activities of these transcription factors.
Title: Gene trapping uncovers sex-specific mechanisms for upstream stimulatory factors 1 and 2 in angiotensinogen expression.

USF-1 but not USF-2 was strongly enriched at Dbeta2 in chromatin from either Rag2-/- double-negative thymocytes or Rag2-/- thymocytes that express a rearranged Tcrb transgene.
Title: DNA double-strand breaks relieve USF-mediated repression of D?2 germline transcription in developing thymocytes.

The following Usf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38133	XM_006539735.3 Latest version!	Mus musculus upstream transcription factor 2 (Usf2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu38133	XM_006539734.3 Latest version!	Mus musculus upstream transcription factor 2 (Usf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13587	NM_011680.2 Latest version!	Mus musculus upstream transcription factor 2 (Usf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu38132	XM_006539733.4 Latest version!	Mus musculus upstream transcription factor 2 (Usf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu38132	XM_006539733.3	Mus musculus upstream transcription factor 2 (Usf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usf2 ( XM_006539734.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539797.1 Mus musculus upstream transcription factor 2 (Usf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu38133
Clone ID Related Accession (Same CDS sequence)	XM_006539734.3 , XM_006539735.3
Accession Version	XM_006539734.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539734.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAC CCCAGGATGT GCTTCAGACA GGCACACAGA GGACAATTGC ACCCCGGACA 
CACCCCTATT CTCCGAAAAT TGATGGAACC AGAACTCCTC GAGATGAGAG AAGAAGAGCT 
CAGCACAATG AAGTGGAACG GAGAAGGAGG GACAAGATCA ACAACTGGAT CGTCCAGCTT 
TCCAAAATCA TTCCAGATTG TCATGCAGAC AACAGCAAGA CAGGAGCAAG TAAAGGGGGC 
ATCCTGTCCA AGGCTTGCGA TTACATCCGG GAGCTACGCC AGACCAACCA GCGCATGCAG 
GAGACCTTCA AGGAGGCAGA GCGGCTGCAG ATGGACAATG AGCTCCTCAG GCAGCAGATC 
GAGGAGCTGA AGAATGAGAA TGCCCTGCTT CGAGCCCAGC TACAGCAGCA CAACCTGGAA 
ATGGTGGGCG AGAGTACCCG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539797.1
CDS	341..787
Translation	MMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ

Target ORF information:

RefSeq Version	XM_006539734.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 2 (Usf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539734.3

ORF Insert Sequence:

ATGATGACAC CCCAGGATGT GCTTCAGACA GGCACACAGA GGACAATTGC ACCCCGGACA 
CACCCCTATT CTCCGAAAAT TGATGGAACC AGAACTCCTC GAGATGAGAG AAGAAGAGCT 
CAGCACAATG AAGTGGAACG GAGAAGGAGG GACAAGATCA ACAACTGGAT CGTCCAGCTT 
TCCAAAATCA TTCCAGATTG TCATGCAGAC AACAGCAAGA CAGGAGCAAG TAAAGGGGGC 
ATCCTGTCCA AGGCTTGCGA TTACATCCGG GAGCTACGCC AGACCAACCA GCGCATGCAG 
GAGACCTTCA AGGAGGCAGA GCGGCTGCAG ATGGACAATG AGCTCCTCAG GCAGCAGATC 
GAGGAGCTGA AGAATGAGAA TGCCCTGCTT CGAGCCCAGC TACAGCAGCA CAACCTGGAA 
ATGGTGGGCG AGAGTACCCG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf2 ( XM_006539735.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539798.1 Mus musculus upstream transcription factor 2 (Usf2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu38133
Clone ID Related Accession (Same CDS sequence)	XM_006539734.3 , XM_006539735.3
Accession Version	XM_006539735.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539735.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAC CCCAGGATGT GCTTCAGACA GGCACACAGA GGACAATTGC ACCCCGGACA 
CACCCCTATT CTCCGAAAAT TGATGGAACC AGAACTCCTC GAGATGAGAG AAGAAGAGCT 
CAGCACAATG AAGTGGAACG GAGAAGGAGG GACAAGATCA ACAACTGGAT CGTCCAGCTT 
TCCAAAATCA TTCCAGATTG TCATGCAGAC AACAGCAAGA CAGGAGCAAG TAAAGGGGGC 
ATCCTGTCCA AGGCTTGCGA TTACATCCGG GAGCTACGCC AGACCAACCA GCGCATGCAG 
GAGACCTTCA AGGAGGCAGA GCGGCTGCAG ATGGACAATG AGCTCCTCAG GCAGCAGATC 
GAGGAGCTGA AGAATGAGAA TGCCCTGCTT CGAGCCCAGC TACAGCAGCA CAACCTGGAA 
ATGGTGGGCG AGAGTACCCG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539798.1
CDS	569..1015
Translation	MMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ

Target ORF information:

RefSeq Version	XM_006539735.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 2 (Usf2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539735.3

ORF Insert Sequence:

ATGATGACAC CCCAGGATGT GCTTCAGACA GGCACACAGA GGACAATTGC ACCCCGGACA 
CACCCCTATT CTCCGAAAAT TGATGGAACC AGAACTCCTC GAGATGAGAG AAGAAGAGCT 
CAGCACAATG AAGTGGAACG GAGAAGGAGG GACAAGATCA ACAACTGGAT CGTCCAGCTT 
TCCAAAATCA TTCCAGATTG TCATGCAGAC AACAGCAAGA CAGGAGCAAG TAAAGGGGGC 
ATCCTGTCCA AGGCTTGCGA TTACATCCGG GAGCTACGCC AGACCAACCA GCGCATGCAG 
GAGACCTTCA AGGAGGCAGA GCGGCTGCAG ATGGACAATG AGCTCCTCAG GCAGCAGATC 
GAGGAGCTGA AGAATGAGAA TGCCCTGCTT CGAGCCCAGC TACAGCAGCA CAACCTGGAA 
ATGGTGGGCG AGAGTACCCG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf2 ( NM_011680.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035810.1 Mus musculus upstream transcription factor 2 (Usf2), mRNA.

Price & Availability↑

CloneID	OMu13587
Clone ID Related Accession (Same CDS sequence)	NM_011680.2
Accession Version	NM_011680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149055.6 and AK146871.1. On Aug 12, 2009 this sequence version replaced NM_011680.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082995.1, U01662.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACATGC TGGACCCGGG TCTGGATCCC GCTTCCTCGG CCACCGCTGC TGCCGCCGCC 
AGCCACGACA AGGGACCCGA GGCCGAGGAG GGCGTTGAGC TGCAGGAAGG CGGGGACGGC 
CCGGGGGCTG AGGAGCAGAC GGCGGTGGCC ATCGCCAGTG TCCAGCAGGC GGCGTTCGGC 
GACCACAATA TCCAGTACCA GTTCCGTACA GAGAGTAATG GAGGCCAGGT GACATACCGC 
GTAGTCCAGG TGACTGATGG TCAGCTGGAC GGCCAGGGCG ACGCGGCCGG CGCCGTCAGC 
GTCGTGTCCA CCGCTGCCTT CGCGGGGGGG CAGCAGGCTG TGACCCAGGT GGGTGTGGAC 
GGGGCAGCCC AGCGCCCCGG CCCTGCCGCT GCCTCTGTAC CCACAGGTCC CGCAGCACCC 
TTCCCGCTGG CTGTAATTCA AAATCCATTC AGCAATGGTG GCAGCCCGGC GGCCGAAGCT 
GTCAGCGGGG AGGCACGATT TGCTTATTTT CCAGCATCCA GTGTGGGCGA TACCACAGCT 
GTGTCTGTTC AAACTACAGA CCAGAGCCTA CAGGCCGGAG GCCAGTTCTA TGTCATGATG 
ACACCCCAGG ATGTGCTTCA GACAGGCACA CAGAGGACAA TTGCACCCCG GACACACCCC 
TATTCTCCGA AAATTGATGG AACCAGAACT CCTCGAGATG AGAGAAGAAG AGCTCAGCAC 
AATGAAGTGG AACGGAGAAG GAGGGACAAG ATCAACAACT GGATCGTCCA GCTTTCCAAA 
ATCATTCCAG ATTGTCATGC AGACAACAGC AAGACAGGAG CAAGTAAAGG GGGCATCCTG 
TCCAAGGCTT GCGATTACAT CCGGGAGCTA CGCCAGACCA ACCAGCGCAT GCAGGAGACC 
TTCAAGGAGG CAGAGCGGCT GCAGATGGAC AATGAGCTCC TCAGGCAGCA GATCGAGGAG 
CTGAAGAATG AGAATGCCCT GCTTCGAGCC CAGCTACAGC AGCACAACCT GGAAATGGTG 
GGCGAGAGTA CCCGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035810.1
CDS	47..1087
Translation	MDMLDPGLDPASSATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAIASVQQAAFGDHNIQYQFRTESNGGQVTYRVVQVTDGQLDGQGDAAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPTGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ

Target ORF information:

RefSeq Version	NM_011680.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 2 (Usf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011680.2

ORF Insert Sequence:

ATGGACATGC TGGACCCGGG TCTGGATCCC GCTTCCTCGG CCACCGCTGC TGCCGCCGCC 
AGCCACGACA AGGGACCCGA GGCCGAGGAG GGCGTTGAGC TGCAGGAAGG CGGGGACGGC 
CCGGGGGCTG AGGAGCAGAC GGCGGTGGCC ATCGCCAGTG TCCAGCAGGC GGCGTTCGGC 
GACCACAATA TCCAGTACCA GTTCCGTACA GAGAGTAATG GAGGCCAGGT GACATACCGC 
GTAGTCCAGG TGACTGATGG TCAGCTGGAC GGCCAGGGCG ACGCGGCCGG CGCCGTCAGC 
GTCGTGTCCA CCGCTGCCTT CGCGGGGGGG CAGCAGGCTG TGACCCAGGT GGGTGTGGAC 
GGGGCAGCCC AGCGCCCCGG CCCTGCCGCT GCCTCTGTAC CCACAGGTCC CGCAGCACCC 
TTCCCGCTGG CTGTAATTCA AAATCCATTC AGCAATGGTG GCAGCCCGGC GGCCGAAGCT 
GTCAGCGGGG AGGCACGATT TGCTTATTTT CCAGCATCCA GTGTGGGCGA TACCACAGCT 
GTGTCTGTTC AAACTACAGA CCAGAGCCTA CAGGCCGGAG GCCAGTTCTA TGTCATGATG 
ACACCCCAGG ATGTGCTTCA GACAGGCACA CAGAGGACAA TTGCACCCCG GACACACCCC 
TATTCTCCGA AAATTGATGG AACCAGAACT CCTCGAGATG AGAGAAGAAG AGCTCAGCAC 
AATGAAGTGG AACGGAGAAG GAGGGACAAG ATCAACAACT GGATCGTCCA GCTTTCCAAA 
ATCATTCCAG ATTGTCATGC AGACAACAGC AAGACAGGAG CAAGTAAAGG GGGCATCCTG 
TCCAAGGCTT GCGATTACAT CCGGGAGCTA CGCCAGACCA ACCAGCGCAT GCAGGAGACC 
TTCAAGGAGG CAGAGCGGCT GCAGATGGAC AATGAGCTCC TCAGGCAGCA GATCGAGGAG 
CTGAAGAATG AGAATGCCCT GCTTCGAGCC CAGCTACAGC AGCACAACCT GGAAATGGTG 
GGCGAGAGTA CCCGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf2 ( XM_006539733.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539796.1 Mus musculus upstream transcription factor 2 (Usf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38132
Clone ID Related Accession (Same CDS sequence)	XM_006539733.3 , XM_006539733.4
Accession Version	XM_006539733.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGC TGGACCCGGG TCTGGATCCC GCTTCCTCGG CCACCGCTGC TGCCGCCGCC 
AGCCACGACA AGGGACCCGA GGCCGAGGAG GGCGTTGAGC TGCAGGAAGG CGGGGACGGC 
CCGGGGGCTG AGGAGCAGAC GGCGGTGGCC ATCGCCAGTG TCCAGCAGGC GGCGTTCGGC 
GACCACAATA TCCAGTACCA GTTCCGTACA GAGAGTAATG GAGGCCAGGC TGTAATTCAA 
AATCCATTCA GCAATGGTGG CAGCCCGGCG GCCGAAGCTG TCAGCGGGGA GGCACGATTT 
GCTTATTTTC CAGCATCCAG TGTGGGCGAT ACCACAGCTG TGTCTGTTCA AACTACAGAC 
CAGAGCCTAC AGGCCGGAGG CCAGTTCTAT GTCATGATGA CACCCCAGGA TGTGCTTCAG 
ACAGGCACAC AGAGGACAAT TGCACCCCGG ACACACCCCT ATTCTCCGAA AATTGATGGA 
ACCAGAACTC CTCGAGATGA GAGAAGAAGA GCTCAGCACA ATGAAGTGGA ACGGAGAAGG 
AGGGACAAGA TCAACAACTG GATCGTCCAG CTTTCCAAAA TCATTCCAGA TTGTCATGCA 
GACAACAGCA AGACAGGAGC AAGTAAAGGG GGCATCCTGT CCAAGGCTTG CGATTACATC 
CGGGAGCTAC GCCAGACCAA CCAGCGCATG CAGGAGACCT TCAAGGAGGC AGAGCGGCTG 
CAGATGGACA ATGAGCTCCT CAGGCAGCAG ATCGAGGAGC TGAAGAATGA GAATGCCCTG 
CTTCGAGCCC AGCTACAGCA GCACAACCTG GAAATGGTGG GCGAGAGTAC CCGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539796.1
CDS	611..1450
Translation	MDMLDPGLDPASSATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAIASVQQAAFGDHNIQYQFRTESNGGQAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ

Target ORF information:

RefSeq Version	XM_006539733.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 2 (Usf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539733.3

ORF Insert Sequence:

ATGGACATGC TGGACCCGGG TCTGGATCCC GCTTCCTCGG CCACCGCTGC TGCCGCCGCC 
AGCCACGACA AGGGACCCGA GGCCGAGGAG GGCGTTGAGC TGCAGGAAGG CGGGGACGGC 
CCGGGGGCTG AGGAGCAGAC GGCGGTGGCC ATCGCCAGTG TCCAGCAGGC GGCGTTCGGC 
GACCACAATA TCCAGTACCA GTTCCGTACA GAGAGTAATG GAGGCCAGGC TGTAATTCAA 
AATCCATTCA GCAATGGTGG CAGCCCGGCG GCCGAAGCTG TCAGCGGGGA GGCACGATTT 
GCTTATTTTC CAGCATCCAG TGTGGGCGAT ACCACAGCTG TGTCTGTTCA AACTACAGAC 
CAGAGCCTAC AGGCCGGAGG CCAGTTCTAT GTCATGATGA CACCCCAGGA TGTGCTTCAG 
ACAGGCACAC AGAGGACAAT TGCACCCCGG ACACACCCCT ATTCTCCGAA AATTGATGGA 
ACCAGAACTC CTCGAGATGA GAGAAGAAGA GCTCAGCACA ATGAAGTGGA ACGGAGAAGG 
AGGGACAAGA TCAACAACTG GATCGTCCAG CTTTCCAAAA TCATTCCAGA TTGTCATGCA 
GACAACAGCA AGACAGGAGC AAGTAAAGGG GGCATCCTGT CCAAGGCTTG CGATTACATC 
CGGGAGCTAC GCCAGACCAA CCAGCGCATG CAGGAGACCT TCAAGGAGGC AGAGCGGCTG 
CAGATGGACA ATGAGCTCCT CAGGCAGCAG ATCGAGGAGC TGAAGAATGA GAATGCCCTG 
CTTCGAGCCC AGCTACAGCA GCACAACCTG GAAATGGTGG GCGAGAGTAC CCGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf2 ( XM_006539733.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539796.1 Mus musculus upstream transcription factor 2 (Usf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38132
Clone ID Related Accession (Same CDS sequence)	XM_006539733.3 , XM_006539733.4
Accession Version	XM_006539733.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539733.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGC TGGACCCGGG TCTGGATCCC GCTTCCTCGG CCACCGCTGC TGCCGCCGCC 
AGCCACGACA AGGGACCCGA GGCCGAGGAG GGCGTTGAGC TGCAGGAAGG CGGGGACGGC 
CCGGGGGCTG AGGAGCAGAC GGCGGTGGCC ATCGCCAGTG TCCAGCAGGC GGCGTTCGGC 
GACCACAATA TCCAGTACCA GTTCCGTACA GAGAGTAATG GAGGCCAGGC TGTAATTCAA 
AATCCATTCA GCAATGGTGG CAGCCCGGCG GCCGAAGCTG TCAGCGGGGA GGCACGATTT 
GCTTATTTTC CAGCATCCAG TGTGGGCGAT ACCACAGCTG TGTCTGTTCA AACTACAGAC 
CAGAGCCTAC AGGCCGGAGG CCAGTTCTAT GTCATGATGA CACCCCAGGA TGTGCTTCAG 
ACAGGCACAC AGAGGACAAT TGCACCCCGG ACACACCCCT ATTCTCCGAA AATTGATGGA 
ACCAGAACTC CTCGAGATGA GAGAAGAAGA GCTCAGCACA ATGAAGTGGA ACGGAGAAGG 
AGGGACAAGA TCAACAACTG GATCGTCCAG CTTTCCAAAA TCATTCCAGA TTGTCATGCA 
GACAACAGCA AGACAGGAGC AAGTAAAGGG GGCATCCTGT CCAAGGCTTG CGATTACATC 
CGGGAGCTAC GCCAGACCAA CCAGCGCATG CAGGAGACCT TCAAGGAGGC AGAGCGGCTG 
CAGATGGACA ATGAGCTCCT CAGGCAGCAG ATCGAGGAGC TGAAGAATGA GAATGCCCTG 
CTTCGAGCCC AGCTACAGCA GCACAACCTG GAAATGGTGG GCGAGAGTAC CCGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539796.1
CDS	195..1034
Translation	MDMLDPGLDPASSATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAIASVQQAAFGDHNIQYQFRTESNGGQAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ

Target ORF information:

RefSeq Version	XM_006539733.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 2 (Usf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539733.4

ORF Insert Sequence:

ATGGACATGC TGGACCCGGG TCTGGATCCC GCTTCCTCGG CCACCGCTGC TGCCGCCGCC 
AGCCACGACA AGGGACCCGA GGCCGAGGAG GGCGTTGAGC TGCAGGAAGG CGGGGACGGC 
CCGGGGGCTG AGGAGCAGAC GGCGGTGGCC ATCGCCAGTG TCCAGCAGGC GGCGTTCGGC 
GACCACAATA TCCAGTACCA GTTCCGTACA GAGAGTAATG GAGGCCAGGC TGTAATTCAA 
AATCCATTCA GCAATGGTGG CAGCCCGGCG GCCGAAGCTG TCAGCGGGGA GGCACGATTT 
GCTTATTTTC CAGCATCCAG TGTGGGCGAT ACCACAGCTG TGTCTGTTCA AACTACAGAC 
CAGAGCCTAC AGGCCGGAGG CCAGTTCTAT GTCATGATGA CACCCCAGGA TGTGCTTCAG 
ACAGGCACAC AGAGGACAAT TGCACCCCGG ACACACCCCT ATTCTCCGAA AATTGATGGA 
ACCAGAACTC CTCGAGATGA GAGAAGAAGA GCTCAGCACA ATGAAGTGGA ACGGAGAAGG 
AGGGACAAGA TCAACAACTG GATCGTCCAG CTTTCCAAAA TCATTCCAGA TTGTCATGCA 
GACAACAGCA AGACAGGAGC AAGTAAAGGG GGCATCCTGT CCAAGGCTTG CGATTACATC 
CGGGAGCTAC GCCAGACCAA CCAGCGCATG CAGGAGACCT TCAAGGAGGC AGAGCGGCTG 
CAGATGGACA ATGAGCTCCT CAGGCAGCAG ATCGAGGAGC TGAAGAATGA GAATGCCCTG 
CTTCGAGCCC AGCTACAGCA GCACAACCTG GAAATGGTGG GCGAGAGTAC CCGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pdx1 and USF transcription factors co-ordinately regulate Alx3 gene expression in pancreatic ?-cells.
The Biochemical journal463(2)287-96(2014 Oct)
Fern?ndez-P?rez A,Vallejo M

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells.
Proteomics13(16)2377-85(2013 Aug)
Zeng L,Sun Y,Xie L,Wei L,Ren Y,Zhao J,Qin W,Mitchelson K,Cheng J

Upstream stimulatory factors 1 and 2 mediate the transcription of angiotensin II binding and inhibitory protein.
The Journal of biological chemistry288(26)19238-49(2013 Jun)
Matsuda M,Tamura K,Wakui H,Maeda A,Ohsawa M,Kanaoka T,Azushima K,Uneda K,Haku S,Tsurumi-Ikeya Y,Toya Y,Maeshima Y,Yamashita A,Umemura S

Differential configurations involving binding of USF transcription factors and Twist1 regulate Alx3 promoter activity in mesenchymal and pancreatic cells.
The Biochemical journal450(1)199-208(2013 Feb)
Garc?a-Sanz P,Fern?ndez-P?rez A,Vallejo M

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Usf2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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