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ALG8 cDNA ORF clone, Saccharomyces cerevisiae S288C

Summary

Gene Symbol ALG8
Entrez Gene ID 854233
Full Name dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase
Synonyms YOR29-18
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001183486.1 NP_014710.1 dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) ALG8 XP_003313272.1
Canis lupus familiaris (dog) ALG8 XP_533998.2
Mus musculus (house mouse) Alg8 NP_950200.2
Danio rerio (zebrafish) alg8 NP_001017647.1
Drosophila melanogaster (fruit fly) CG4542 NP_572355.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP003928 XP_318380.5
Schizosaccharomyces pombe (fission yeast) alg8 NP_594599.1
Saccharomyces cerevisiae (baker's yeast) ALG8 NP_014710.1
Neurospora crassa NCU00163 XP_956676.1
Arabidopsis thaliana (thale cress) AT2G44660 NP_181994.5
Xenopus tropicalis (tropical clawed frog) alg8 NP_001076820.1
Gallus gallus (chicken) ALG8 XP_004938993.1
Kluyveromyces lactis KLLA0F16621g XP_455827.1
Eremothecium gossypii AGOS_ADR210C NP_984306.2
Homo sapiens (human) ALG8 NP_076984.2
Rattus norvegicus (Norway rat) Alg8 NP_001029299.1
Bos taurus (cattle) ALG8 NP_001069593.1
Magnaporthe oryzae (rice blast fungus) MGG_05686 XP_003710585.1
Oryza sativa (rice) Os02g0688500 NP_001047779.1

Gene Ontology


Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ALG8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALG8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03375 NM_001183486.1
Latest version!		 Saccharomyces cerevisiae S288C dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase (ALG8), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSi03375
Clone ID Related Accession (Same CDS sequence) NM_001183486.1
Accession Version NM_001183486.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1734bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-31
Organism Saccharomyces cerevisiae S288C
Product dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase
Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

1
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361
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481
541
601
661
721
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841
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961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
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ATGAAAGGTG ATCGTTCGAG GCAAAATATG GCTGTGACAA AGAAGGCAAA GTTAAAAAAA 
AATGACGAGC CAAAAAAGGT TTTGAAGACT GCCGCGACCG AAAAAGGTGA GGGATCCAAG 
CGCTACTCCT TATGGAATTT CTGGATAAGC ACATTGTTTT TAAAATTACT ATTGATTCCT 
GACTACTTCA GTACAGATTT TGACGTTCAT AGAAATTGGC TGGCAATCAC CAATAAATTG 
CCCATCAGCG AATGGTATTA TGAGCACACT AGTCAATGGA CATTGGATTA TCCGCCATTT 
TTTGCTTATT TTGAATGGTT TTTATCGCAA TTTGTTCCTA AATCTGTACG TGATGACGGT 
TGTTTGGATA TAGTGGAGAT CGGAAAGTTC GGTTTGCCAA CAATCGTTTT CCAACGACTC 
ACTGTCATCT TCAGCGAAAT TCTTCTATTT GTCATCCTTC AAATATACAT TAATACTACA 
AAGCTAAGTG AAAGATCTCA AAGTTTTGTG GTTGCTTCTA GCATTGTACT ATCACCCGGG 
TTTTTAATAA TAGATCACAT TCACTTCCAA TATAATGGTT TTCTTTTTGC GATTCTAATA 
GGCTCCATTG TCGCCGCCAA GAATAAGAGA TACATCTTGT GCGCTGTTCT GTATACTACA 
GCCATATGTT TCAAGCATAT ATTTTTATAT TTGGCGCCAT GTTATTTCGT GTTTTTATTG 
AGAGCATACG TTCTCAATGT CAACAATTTC AAGTTTAAAA GTTACAAAGA TTTTTTGTTC 
CTGATAAGAT GGGCAAATCT TTTGAAGTTG GCTACCGTTG TTGTTGGAAT TTTCACTATT 
TGTTTCCTTC CATTTGCTCA TCAAATGCCG CAAGTGTTGA GCAGGTTATT CCCATTCTCA 
AGAGGACTGA CGCATGCATA TTGGGCACCA AATTTCTGGG CGTTGTATTC TTTTATGGAT 
AAAATACTCA CTACGGTAAT GTTGAAATTA CCGTACGTCC ACACTTTTGC TACAAAATTT 
ATTAAACCTC CTCTGATCCC TCAGAATATC AAGGAAATTA ATGAAAGGCT CGCGGCAAAC 
AACAATGGAA GTAAAGGTCT AGTTCAAGAC GTATTTTTTG TTATTCTCCC TCAAATTCCT 
CCAAAATTAA CGTTTATTTT GACGATATTT TACCAGGTCT TAGCAGTACT TCCACTTTTG 
TTTGATCCAT CGTTCAAAAG ATTTGTTGGC TCGTTGACTC TCTGTGGGTT GGCATCGTTT 
TTGTTCGGGT GGCATGTGCA TGAGAAAGCT ATAATGCTAG TCATCATTCC GTTCACATTT 
CTTGTTGGAT TTGATCGTCG TCTTTTGGTA CCGTTTATGT TAGTTGCCTC AGCTGGTTAT 
GTTTCCTTGT ATCCTCTTCT GTACAAAGGC CAAGATTTTT TCATTAAAAC ATTATACACT 
TATGTTTGGT GTATAATTTA TTTTGCCGCT TTTAGAAAAA CTACGAAGAT TTCTTCAAGT 
GTAGAAAGAA GAATCTTTTT CTTGGATAGG TTGGCATTAA CGTACATCTT TTCATTGTTA 
CCAATCGTCA CCGTATTACA AATATTGGAT GAAGTCAAGT GGAGATATTC ATTTTTGCAA 
AAATTTGAAT TCTTAGGATT AATGATATAT AGTGTCTATT GTTCATTAGG TATAATCAGC 
TCGTGGTTTG CCTTATCGTG GTTATATAAT TTTGACGAAT TACTATGGCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_014710.1
CDS1..1734
Translation

Target ORF information:

RefSeq Version NM_001183486.1
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288C dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase (ALG8), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001183486.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGAAAGGTG ATCGTTCGAG GCAAAATATG GCTGTGACAA AGAAGGCAAA GTTAAAAAAA 
AATGACGAGC CAAAAAAGGT TTTGAAGACT GCCGCGACCG AAAAAGGTGA GGGATCCAAG 
CGCTACTCCT TATGGAATTT CTGGATAAGC ACATTGTTTT TAAAATTACT ATTGATTCCT 
GACTACTTCA GTACAGATTT TGACGTTCAT AGAAATTGGC TGGCAATCAC CAATAAATTG 
CCCATCAGCG AATGGTATTA TGAGCACACT AGTCAATGGA CATTGGATTA TCCGCCATTT 
TTTGCTTATT TTGAATGGTT TTTATCGCAA TTTGTTCCTA AATCTGTACG TGATGACGGT 
TGTTTGGATA TAGTGGAGAT CGGAAAGTTC GGTTTGCCAA CAATCGTTTT CCAACGACTC 
ACTGTCATCT TCAGCGAAAT TCTTCTATTT GTCATCCTTC AAATATACAT TAATACTACA 
AAGCTAAGTG AAAGATCTCA AAGTTTTGTG GTTGCTTCTA GCATTGTACT ATCACCCGGG 
TTTTTAATAA TAGATCACAT TCACTTCCAA TATAATGGTT TTCTTTTTGC GATTCTAATA 
GGCTCCATTG TCGCCGCCAA GAATAAGAGA TACATCTTGT GCGCTGTTCT GTATACTACA 
GCCATATGTT TCAAGCATAT ATTTTTATAT TTGGCGCCAT GTTATTTCGT GTTTTTATTG 
AGAGCATACG TTCTCAATGT CAACAATTTC AAGTTTAAAA GTTACAAAGA TTTTTTGTTC 
CTGATAAGAT GGGCAAATCT TTTGAAGTTG GCTACCGTTG TTGTTGGAAT TTTCACTATT 
TGTTTCCTTC CATTTGCTCA TCAAATGCCG CAAGTGTTGA GCAGGTTATT CCCATTCTCA 
AGAGGACTGA CGCATGCATA TTGGGCACCA AATTTCTGGG CGTTGTATTC TTTTATGGAT 
AAAATACTCA CTACGGTAAT GTTGAAATTA CCGTACGTCC ACACTTTTGC TACAAAATTT 
ATTAAACCTC CTCTGATCCC TCAGAATATC AAGGAAATTA ATGAAAGGCT CGCGGCAAAC 
AACAATGGAA GTAAAGGTCT AGTTCAAGAC GTATTTTTTG TTATTCTCCC TCAAATTCCT 
CCAAAATTAA CGTTTATTTT GACGATATTT TACCAGGTCT TAGCAGTACT TCCACTTTTG 
TTTGATCCAT CGTTCAAAAG ATTTGTTGGC TCGTTGACTC TCTGTGGGTT GGCATCGTTT 
TTGTTCGGGT GGCATGTGCA TGAGAAAGCT ATAATGCTAG TCATCATTCC GTTCACATTT 
CTTGTTGGAT TTGATCGTCG TCTTTTGGTA CCGTTTATGT TAGTTGCCTC AGCTGGTTAT 
GTTTCCTTGT ATCCTCTTCT GTACAAAGGC CAAGATTTTT TCATTAAAAC ATTATACACT 
TATGTTTGGT GTATAATTTA TTTTGCCGCT TTTAGAAAAA CTACGAAGAT TTCTTCAAGT 
GTAGAAAGAA GAATCTTTTT CTTGGATAGG TTGGCATTAA CGTACATCTT TTCATTGTTA 
CCAATCGTCA CCGTATTACA AATATTGGAT GAAGTCAAGT GGAGATATTC ATTTTTGCAA 
AAATTTGAAT TCTTAGGATT AATGATATAT AGTGTCTATT GTTCATTAGG TATAATCAGC 
TCGTGGTTTG CCTTATCGTG GTTATATAAT TTTGACGAAT TACTATGGCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K


book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG