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GCD6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Summary

Gene Symbol GCD6
Entrez Gene ID 851797
Full Name translation initiation factor eIF2B catalytic subunit epsilon
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001180519.3 NP_010497.3 translation initiation factor eIF2B catalytic subunit epsilon

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) EIF2B5 XP_001139517.1
Gallus gallus (chicken) EIF2B5 XP_422755.3
Drosophila melanogaster (fruit fly) eIF2B-epsilon NP_996329.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000082 XP_001687758.1
Arabidopsis thaliana (thale cress) AT2G34970 NP_181042.1
EIF2B5 XP_001104122.1
Danio rerio (zebrafish) eif2b5 NP_001107069.1
Schizosaccharomyces pombe (fission yeast) tif225 NP_594285.1
Arabidopsis thaliana (thale cress) AT4G18300 NP_193564.1
Xenopus tropicalis (tropical clawed frog) eif2b5 XP_002935185.2
Mus musculus (house mouse) Eif2b5 NP_758469.1
Saccharomyces cerevisiae (baker's yeast) GCD6 NP_010497.3
Eremothecium gossypii AGOS_AGL094W NP_986572.1
Canis lupus familiaris (dog) EIF2B5 XP_003640192.1
Bos taurus (cattle) EIF2B5 XP_002684924.1
Rattus norvegicus (Norway rat) Eif2b5 NP_620221.2
Kluyveromyces lactis KLLA0A04235g XP_451187.1
Neurospora crassa NCU02414 XP_959596.1
Homo sapiens (human) EIF2B5 NP_003898.2
Magnaporthe oryzae (rice blast fungus) MGG_03517 XP_003716402.1
Arabidopsis thaliana (thale cress) AT3G02270 NP_186876.1
Oryza sativa (rice) Os02g0812400 NP_001048478.1

Gene Ontology


Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data show that multiple contacts between eIF2gamma and eIF2Bepsilon mediate nucleotide exchange.
Title: Critical contacts between the eukaryotic initiation factor 2B (eIF2B) catalytic domain and both eIF2beta and -2gamma mediate guanine nucleotide exchange.

The following GCD6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCD6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01294 NM_001180519.3
Latest version!		 Saccharomyces cerevisiae S288C translation initiation factor eIF2B catalytic subunit epsilon (GCD6), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSi01294
Clone ID Related Accession (Same CDS sequence) NM_001180519.3
Accession Version NM_001180519.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-31
Organism Saccharomyces cerevisiae S288C
Product translation initiation factor eIF2B catalytic subunit epsilon
Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180519.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

1
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541
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661
721
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841
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961
1021
1081
1141
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1261
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1381
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1501
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1801
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1921
1981
2041
2101
ATGGCTGGAA AAAAGGGACA AAAGAAAAGT GGACTAGGCA ACCATGGAAA GAACTCTGAT 
ATGGATGTTG AAGATCGTCT CCAGGCCGTT GTCTTGACAG ACTCTTATGA AACTAGGTTT 
ATGCCACTGA CAGCTGTCAA GCCAAGGTGT TTGCTGCCAC TGGCTAACGT ACCTCTCATT 
GAATACACCT TAGAATTTTT GGCTAAGGCT GGCGTACATG AAGTTTTCTT AATTTGCTCT 
TCTCATGCCA ACCAAATTAA TGACTATATT GAGAATTCTA AGTGGAACTT GCCTTGGTCT 
CCATTTAAAA TTACCACCAT TATGTCTCCA GAAGCTAGAT GTACGGGTGA TGTTATGAGA 
GATCTAGATA ATAGAGGTAT CATTACTGGA GATTTTATTT TAGTCAGTGG TGATGTATTG 
ACTAACATCG ATTTCAGCAA AATGCTAGAA TTTCACAAAA AAATGCATTT GCAAGATAAA 
GATCACATCT CGACAATGTG TTTGAGCAAA GCGAGTACCT ATCCAAAAAC AAGAACTATT 
GAGCCTGCCG CATTTGTCTT AGACAAATCC ACCAGTAGGT GTATTTATTA CCAAGATTTA 
CCATTACCAA GCTCTAGAGA GAAGACTTCC ATTCAGATTG ACCCAGAATT GTTGGATAAT 
GTCGATGAAT TTGTCATAAG GAATGACCTC ATCGATTGTA GAATTGACAT TTGTACATCT 
CATGTACCTT TGATATTTCA AGAAAATTTT GACTACCAAT CATTAAGGAC AGACTTTGTT 
AAAGGTGTCA TTTCAAGCGA TATATTGGGA AAGCATATAT ATGCCTATTT GACGGACGAA 
TATGCTGTAA GAGTTGAAAG TTGGCAAACT TACGACACCA TTTCTCAAGA CTTTTTAGGT 
AGATGGTGTT ATCCCTTGGT CTTAGACTCT AACATACAGG ACGATCAAAC GTATTCTTAT 
GAATCAAGAC ATATATACAA GGAAAAAGAC GTTGTTTTGG CCCAATCCTG TAAAATTGGT 
AAGTGCACTG CAATTGGATC AGGAACAAAA ATCGGAGAGG GTACGAAAAT TGAGAATTCC 
GTGATTGGAA GGAACTGCCA AATCGGTGAA AATATTAGAA TCAAGAACAG TTTCATTTGG 
GATGACTGTA TCATCGGAAA TAACAGTATA ATTGACCATT CATTAATTGC CTCTAATGCC 
ACGTTAGGGA GTAATGTACG CCTAAATGAT GGCTGTATAA TTGGTTTCAA CGTTAAAATT 
GATGATAATA TGGATTTAGA TAGAAACACA AAAATATCTG CCAGTCCATT AAAAAATGCC 
GGCTCAAGAA TGTATGATAA TGAAAGCAAT GAGCAGTTTG ACCAAGACCT TGATGATCAG 
ACACTAGCCG TTTCTATTGT TGGAGATAAG GGTGTTGGTT ATATTTACGA AAGCGAGGTG 
TCTGACGATG AAGATAGTTC TACAGAAGCC TGCAAAGAAA TAAACACTTT GAGTAACCAA 
TTAGATGAGT TATACTTAAG TGACGATTCA ATTTCTTCCG CCACTAAAAA GACAAAGAAA 
AGAAGAACGA TGTCTGTCAA TAGTATATAC ACGGATAGAG AAGAAATTGA TTCTGAATTT 
GAGGACGAAG ATTTTGAGAA AGAAGGTATT GCCACCGTGG AGCGTGCTAT GGAAAACAAT 
CATGATCTTG ACACAGCATT ATTAGAATTG AACACCTTGA GAATGAGTAT GAACGTGACA 
TATCATGAGG TGAGAATAGC AACAATAACT GCATTATTGA GAAGAGTTTA CCACTTTATT 
GCAACTCAAA CATTAGGTCC CAAGGACGCT GTGGTGAAGG TTTTTAATCA GTGGGGACTG 
TTGTTCAAGA GACAAGCCTT TGATGAAGAA GAGTATATTG ATTTGATGAA CATCATAATG 
GAAAAAATTG TAGAACAGAG TTTTGACAAA CCGGATTTGA TTCTATTTAG TGCATTGGTT 
TCTCTATACG ATAATGACAT AATTGAGGAG GATGTCATTT ATAAATGGTG GGATAATGTT 
TCTACTGACC CTCGCTATGA TGAAGTCAAG AAATTAACTG TAAAGTGGGT TGAGTGGTTA 
CAGAATGCTG ACGAAGAATC TTCCTCAGAA GAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_010497.3
CDS1..2139
Translation

Target ORF information:

RefSeq Version NM_001180519.3
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288C translation initiation factor eIF2B catalytic subunit epsilon (GCD6), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001180519.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCTGGAA AAAAGGGACA AAAGAAAAGT GGACTAGGCA ACCATGGAAA GAACTCTGAT 
ATGGATGTTG AAGATCGTCT CCAGGCCGTT GTCTTGACAG ACTCTTATGA AACTAGGTTT 
ATGCCACTGA CAGCTGTCAA GCCAAGGTGT TTGCTGCCAC TGGCTAACGT ACCTCTCATT 
GAATACACCT TAGAATTTTT GGCTAAGGCT GGCGTACATG AAGTTTTCTT AATTTGCTCT 
TCTCATGCCA ACCAAATTAA TGACTATATT GAGAATTCTA AGTGGAACTT GCCTTGGTCT 
CCATTTAAAA TTACCACCAT TATGTCTCCA GAAGCTAGAT GTACGGGTGA TGTTATGAGA 
GATCTAGATA ATAGAGGTAT CATTACTGGA GATTTTATTT TAGTCAGTGG TGATGTATTG 
ACTAACATCG ATTTCAGCAA AATGCTAGAA TTTCACAAAA AAATGCATTT GCAAGATAAA 
GATCACATCT CGACAATGTG TTTGAGCAAA GCGAGTACCT ATCCAAAAAC AAGAACTATT 
GAGCCTGCCG CATTTGTCTT AGACAAATCC ACCAGTAGGT GTATTTATTA CCAAGATTTA 
CCATTACCAA GCTCTAGAGA GAAGACTTCC ATTCAGATTG ACCCAGAATT GTTGGATAAT 
GTCGATGAAT TTGTCATAAG GAATGACCTC ATCGATTGTA GAATTGACAT TTGTACATCT 
CATGTACCTT TGATATTTCA AGAAAATTTT GACTACCAAT CATTAAGGAC AGACTTTGTT 
AAAGGTGTCA TTTCAAGCGA TATATTGGGA AAGCATATAT ATGCCTATTT GACGGACGAA 
TATGCTGTAA GAGTTGAAAG TTGGCAAACT TACGACACCA TTTCTCAAGA CTTTTTAGGT 
AGATGGTGTT ATCCCTTGGT CTTAGACTCT AACATACAGG ACGATCAAAC GTATTCTTAT 
GAATCAAGAC ATATATACAA GGAAAAAGAC GTTGTTTTGG CCCAATCCTG TAAAATTGGT 
AAGTGCACTG CAATTGGATC AGGAACAAAA ATCGGAGAGG GTACGAAAAT TGAGAATTCC 
GTGATTGGAA GGAACTGCCA AATCGGTGAA AATATTAGAA TCAAGAACAG TTTCATTTGG 
GATGACTGTA TCATCGGAAA TAACAGTATA ATTGACCATT CATTAATTGC CTCTAATGCC 
ACGTTAGGGA GTAATGTACG CCTAAATGAT GGCTGTATAA TTGGTTTCAA CGTTAAAATT 
GATGATAATA TGGATTTAGA TAGAAACACA AAAATATCTG CCAGTCCATT AAAAAATGCC 
GGCTCAAGAA TGTATGATAA TGAAAGCAAT GAGCAGTTTG ACCAAGACCT TGATGATCAG 
ACACTAGCCG TTTCTATTGT TGGAGATAAG GGTGTTGGTT ATATTTACGA AAGCGAGGTG 
TCTGACGATG AAGATAGTTC TACAGAAGCC TGCAAAGAAA TAAACACTTT GAGTAACCAA 
TTAGATGAGT TATACTTAAG TGACGATTCA ATTTCTTCCG CCACTAAAAA GACAAAGAAA 
AGAAGAACGA TGTCTGTCAA TAGTATATAC ACGGATAGAG AAGAAATTGA TTCTGAATTT 
GAGGACGAAG ATTTTGAGAA AGAAGGTATT GCCACCGTGG AGCGTGCTAT GGAAAACAAT 
CATGATCTTG ACACAGCATT ATTAGAATTG AACACCTTGA GAATGAGTAT GAACGTGACA 
TATCATGAGG TGAGAATAGC AACAATAACT GCATTATTGA GAAGAGTTTA CCACTTTATT 
GCAACTCAAA CATTAGGTCC CAAGGACGCT GTGGTGAAGG TTTTTAATCA GTGGGGACTG 
TTGTTCAAGA GACAAGCCTT TGATGAAGAA GAGTATATTG ATTTGATGAA CATCATAATG 
GAAAAAATTG TAGAACAGAG TTTTGACAAA CCGGATTTGA TTCTATTTAG TGCATTGGTT 
TCTCTATACG ATAATGACAT AATTGAGGAG GATGTCATTT ATAAATGGTG GGATAATGTT 
TCTACTGACC CTCGCTATGA TGAAGTCAAG AAATTAACTG TAAAGTGGGT TGAGTGGTTA 
CAGAATGCTG ACGAAGAATC TTCCTCAGAA GAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P


book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG