• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

GLN4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Summary

Gene Symbol GLN4
Entrez Gene ID 854339
Full Name glutamine--tRNA ligase
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001183587.3 NP_014811.3 glutamine--tRNA ligase

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) QARS XP_005632622.1
Bos taurus (cattle) QARS NP_001029640.1
Eremothecium gossypii AGOS_AGR259C NP_986925.1
Magnaporthe oryzae (rice blast fungus) MGG_09393 XP_003720218.1
Oryza sativa (rice) Os01g0185200 NP_001042238.1
Gallus gallus (chicken) QARS NP_001012800.1
Saccharomyces cerevisiae (baker's yeast) GLN4 NP_014811.3
Xenopus tropicalis (tropical clawed frog) qars XP_004914154.1
Danio rerio (zebrafish) qars NP_957507.1
Caenorhabditis elegans (roundworm) qars-1 NP_001255843.1
Schizosaccharomyces pombe (fission yeast) qrs1 NP_596745.1
Homo sapiens (human) QARS NP_005042.1
Pan troglodytes (chimpanzee) QARS XP_001147632.3
Drosophila melanogaster (fruit fly) Aats-gln NP_524841.1
Kluyveromyces lactis KLLA0F20449g XP_455998.1
QARS XP_001110256.2
Rattus norvegicus (Norway rat) LOC100911132 XP_003752277.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP010267 XP_319458.3
Neurospora crassa NCU07926 XP_962442.1
Arabidopsis thaliana (thale cress) OVA9 NP_173906.2
Oryza sativa (rice) Os05g0182800 NP_001054822.1

Gene Ontology


Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the eukaryote-specific protein domains of GluRS and GlnRS substantially influence amino acid binding, tRNA binding and aminoacylation efficiency, but they play no role in either specific nucleotide readout or discrimination against noncognate tRNA
Title: Coevolution of specificity determinants in eukaryotic glutamyl- and glutaminyl-tRNA synthetases.

The structure of the C-terminal domain is highly similar to Escherichia coli GlnRS but 214 residues, including the N-terminal domain, are crystallographically disordered.
Title: The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA.

A functional and structural analysis of the N-terminal appended domain of Saccharomyces cerevisiae GlnRS, Gln4.
Title: Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase.

The following GLN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03469 NM_001183587.3
Latest version!		 Saccharomyces cerevisiae S288C glutamine--tRNA ligase (GLN4), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSi03469
Clone ID Related Accession (Same CDS sequence) NM_001183587.3
Accession Version NM_001183587.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-31
Organism Saccharomyces cerevisiae S288C
Product glutamine--tRNA ligase
Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). On Jul 30, 2012 this sequence version replaced NM_001183587.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTCTTCTG TAGAAGAATT GACTCAGCTG TTTTCACAGG TTGGGTTTGA AGATAAAAAG 
GTGAAGGAGA TTGTCAAGAA TAAAAAGGTT TCTGATTCAC TTTACAAATT GATTAAGGAG 
ACTCCTTCGG ACTATCAATG GAACAAATCT ACTAGAGCGC TAGTACACAA CCTGGCATCT 
TTTGTCAAAG GTACAGATCT ACCCAAGTCT GAATTAATTG TTAACGGCAT CATTAATGGT 
GATTTGAAGA CATCTCTGCA AGTCGATGCC GCATTTAAAT ATGTAAAGGC TAATGGCGAG 
GCATCCACCA AAATGGGTAT GAATGAAAAC TCTGGTGTTG GTATTGAGAT TACAGAGGAT 
CAGGTTAGAA ATTACGTCAT GCAGTACATT CAAGAAAACA AGGAAAGAAT TTTAACTGAA 
CGCTATAAAC TGGTGCCAGG TATTTTTGCC GATGTTAAAA ACTTAAAGGA GCTGAAGTGG 
GCCGATCCAC GTAGTTTCAA GCCAATTATC GACCAAGAAG TCTTAAAACT TTTGGGTCCA 
AAGGATGAGA GAGACTTAAT CAAGAAGAAG ACCAAGAATA ATGAAAAGAA GAAAACCAAT 
TCTGCCAAGA AGAGTTCTGA TAATTCTGCA TCATCCGGTC CAAAGAGGAC TATGTTCAAT 
GAAGGTTTCC TAGGTGATTT GCATAAAGTG GGTGAAAACC CACAAGCTTA CCCAGAATTA 
ATGAAGGAAC ACCTTGAGGT TACTGGTGGC AAAGTTCGTA CAAGGTTCCC TCCGGAGCCC 
AATGGATATT TGCATATTGG TCATTCTAAA GCTATTATGG TTAATTTTGG CTATGCTAAA 
TATCACAATG GTACCTGTTA TTTAAGATTT GACGATACCA ACCCCGAAAA GGAAGCTCCT 
GAATATTTTG AATCCATTAA GAGAATGGTT TCTTGGTTAG GTTTTAAACC ATGGAAAATT 
ACTTACTCAA GTGATTATTT TGACGAGCTT TATCGCCTCG CTGAAGTGTT GATCAAAAAC 
GGCAAAGCTT ACGTTTGTCA TTGTACCGCC GAAGAAATTA AAAGAGGTCG TGGTATTAAA 
GAAGACGGTA CTCCAGGTGG GGAGAGATAT GCTTGTAAGC ATCGTGATCA GTCGATAGAA 
CAAAACCTGC AAGAATTCAG AGATATGAGA GATGGCAAGT ACAAACCTGG TGAAGCTATC 
TTAAGAATGA AACAGGATCT AAATTCACCA AGCCCACAAA TGTGGGACTT GATTGCGTAT 
AGAGTTTTAA ATGCTCCACA TCCAAGAACG GGAACAAAAT GGAGAATTTA TCCTACTTAC 
GATTTTACAC ATTGTTTGGT CGATTCAATG GAAAATATTA CACATTCTTT ATGTACTACT 
GAATTCTACC TATCTAGAGA AAGCTATGAA TGGTTATGTG ATCAGGTTCA TGTGTTCAGA 
CCCGCTCAAA GAGAATATGG TCGTTTGAAT ATCACCGGTA CAGTTTTATC CAAAAGAAAA 
ATTGCGCAAT TGGTGGATGA AAAATTCGTT AGAGGCTGGG ACGATCCTAG ACTATTCACG 
CTTGAAGCTA TACGCAGACG TGGTGTTCCT CCTGGTGCCA TTTTATCCTT TATCAACACA 
TTAGGTGTTA CCACAAGTAC CACAAACATT CAAGTTGTGA GGTTTGAGAG CGCTGTTAGA 
AAGTACCTAG AAGATACCAC ACCGAGATTA ATGTTTGTGT TGGATCCTGT TGAAGTTGTT 
GTCGATAATT TATCTGACGA TTATGAAGAA TTGGCAACTA TTCCTTACAG ACCTGGTACT 
CCAGAATTTG GTGAAAGAAC TGTACCATTT ACAAACAAAT TCTACATTGA AAGGTCAGAT 
TTCTCAGAAA ACGTTGATGA TAAGGAATTT TTCAGATTAA CACCAAACCA ACCAGTTGGT 
TTAATTAAAG TCTCACACAC AGTTTCTTTC AAAAGTTTGG AAAAAGATGA AGCTGGTAAA 
ATTATAAGAA TTCATGTCAA CTATGATAAC AAGGTTGAAG AAGGTTCGAA ACCAAAGAAA 
CCAAAAACTT ATATTCAATG GGTTCCTATC TCTTCAAAAT ATAATTCTCC ATTGAGAGTT 
ACTGAAACTA GGGTTTACAA CCAATTGTTC AAATCTGAAA ATCCATCTTC TCATCCTGAA 
GGCTTCTTGA AGGACATCAA CCCAGAAAGT GAAGTCGTTT ATAAAGAATC TGTCATGGAA 
CATAACTTCG GGGACGTTGT CAAGAACAGT CCATGGGTCG TTGATTCTGT TAAAAACTCG 
GAATTTTATG TTGAAGAAGA TAAGGATTCC AAAGAAGTGT GTAGATTCCA AGCTATGAGA 
GTAGGTTATT TCACTCTGGA TAAAGAGAGT ACGACTTCTA AGGTTATTTT GAATAGAATA 
GTTAGCTTGA AGGACGCAAC TTCCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_014811.3
CDS1..2430
Translation

Target ORF information:

RefSeq Version NM_001183587.3
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288C glutamine--tRNA ligase (GLN4), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001183587.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTCTTCTG TAGAAGAATT GACTCAGCTG TTTTCACAGG TTGGGTTTGA AGATAAAAAG 
GTGAAGGAGA TTGTCAAGAA TAAAAAGGTT TCTGATTCAC TTTACAAATT GATTAAGGAG 
ACTCCTTCGG ACTATCAATG GAACAAATCT ACTAGAGCGC TAGTACACAA CCTGGCATCT 
TTTGTCAAAG GTACAGATCT ACCCAAGTCT GAATTAATTG TTAACGGCAT CATTAATGGT 
GATTTGAAGA CATCTCTGCA AGTCGATGCC GCATTTAAAT ATGTAAAGGC TAATGGCGAG 
GCATCCACCA AAATGGGTAT GAATGAAAAC TCTGGTGTTG GTATTGAGAT TACAGAGGAT 
CAGGTTAGAA ATTACGTCAT GCAGTACATT CAAGAAAACA AGGAAAGAAT TTTAACTGAA 
CGCTATAAAC TGGTGCCAGG TATTTTTGCC GATGTTAAAA ACTTAAAGGA GCTGAAGTGG 
GCCGATCCAC GTAGTTTCAA GCCAATTATC GACCAAGAAG TCTTAAAACT TTTGGGTCCA 
AAGGATGAGA GAGACTTAAT CAAGAAGAAG ACCAAGAATA ATGAAAAGAA GAAAACCAAT 
TCTGCCAAGA AGAGTTCTGA TAATTCTGCA TCATCCGGTC CAAAGAGGAC TATGTTCAAT 
GAAGGTTTCC TAGGTGATTT GCATAAAGTG GGTGAAAACC CACAAGCTTA CCCAGAATTA 
ATGAAGGAAC ACCTTGAGGT TACTGGTGGC AAAGTTCGTA CAAGGTTCCC TCCGGAGCCC 
AATGGATATT TGCATATTGG TCATTCTAAA GCTATTATGG TTAATTTTGG CTATGCTAAA 
TATCACAATG GTACCTGTTA TTTAAGATTT GACGATACCA ACCCCGAAAA GGAAGCTCCT 
GAATATTTTG AATCCATTAA GAGAATGGTT TCTTGGTTAG GTTTTAAACC ATGGAAAATT 
ACTTACTCAA GTGATTATTT TGACGAGCTT TATCGCCTCG CTGAAGTGTT GATCAAAAAC 
GGCAAAGCTT ACGTTTGTCA TTGTACCGCC GAAGAAATTA AAAGAGGTCG TGGTATTAAA 
GAAGACGGTA CTCCAGGTGG GGAGAGATAT GCTTGTAAGC ATCGTGATCA GTCGATAGAA 
CAAAACCTGC AAGAATTCAG AGATATGAGA GATGGCAAGT ACAAACCTGG TGAAGCTATC 
TTAAGAATGA AACAGGATCT AAATTCACCA AGCCCACAAA TGTGGGACTT GATTGCGTAT 
AGAGTTTTAA ATGCTCCACA TCCAAGAACG GGAACAAAAT GGAGAATTTA TCCTACTTAC 
GATTTTACAC ATTGTTTGGT CGATTCAATG GAAAATATTA CACATTCTTT ATGTACTACT 
GAATTCTACC TATCTAGAGA AAGCTATGAA TGGTTATGTG ATCAGGTTCA TGTGTTCAGA 
CCCGCTCAAA GAGAATATGG TCGTTTGAAT ATCACCGGTA CAGTTTTATC CAAAAGAAAA 
ATTGCGCAAT TGGTGGATGA AAAATTCGTT AGAGGCTGGG ACGATCCTAG ACTATTCACG 
CTTGAAGCTA TACGCAGACG TGGTGTTCCT CCTGGTGCCA TTTTATCCTT TATCAACACA 
TTAGGTGTTA CCACAAGTAC CACAAACATT CAAGTTGTGA GGTTTGAGAG CGCTGTTAGA 
AAGTACCTAG AAGATACCAC ACCGAGATTA ATGTTTGTGT TGGATCCTGT TGAAGTTGTT 
GTCGATAATT TATCTGACGA TTATGAAGAA TTGGCAACTA TTCCTTACAG ACCTGGTACT 
CCAGAATTTG GTGAAAGAAC TGTACCATTT ACAAACAAAT TCTACATTGA AAGGTCAGAT 
TTCTCAGAAA ACGTTGATGA TAAGGAATTT TTCAGATTAA CACCAAACCA ACCAGTTGGT 
TTAATTAAAG TCTCACACAC AGTTTCTTTC AAAAGTTTGG AAAAAGATGA AGCTGGTAAA 
ATTATAAGAA TTCATGTCAA CTATGATAAC AAGGTTGAAG AAGGTTCGAA ACCAAAGAAA 
CCAAAAACTT ATATTCAATG GGTTCCTATC TCTTCAAAAT ATAATTCTCC ATTGAGAGTT 
ACTGAAACTA GGGTTTACAA CCAATTGTTC AAATCTGAAA ATCCATCTTC TCATCCTGAA 
GGCTTCTTGA AGGACATCAA CCCAGAAAGT GAAGTCGTTT ATAAAGAATC TGTCATGGAA 
CATAACTTCG GGGACGTTGT CAAGAACAGT CCATGGGTCG TTGATTCTGT TAAAAACTCG 
GAATTTTATG TTGAAGAAGA TAAGGATTCC AAAGAAGTGT GTAGATTCCA AGCTATGAGA 
GTAGGTTATT TCACTCTGGA TAAAGAGAGT ACGACTTCTA AGGTTATTTT GAATAGAATA 
GTTAGCTTGA AGGACGCAAC TTCCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K


book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG