LOC578329 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC578329
Entrez Gene ID	578329
Full Name	E3 ubiquitin-protein ligase TRIP12
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030981766.1	XP_030837626.1	E3 ubiquitin-protein ligase TRIP12
XM_011677706.1	XP_011676008.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011677707.1	XP_011676009.1	E3 ubiquitin-protein ligase TRIP12 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC578329 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC578329 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd22243	XM_011677707.1 Latest version!	Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSd48011	XM_030981766.1 Latest version!	Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSd22242	XM_011677706.1 Latest version!	Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC578329 ( XM_011677707.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011676009.1 Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), transcript variant X2, mRNA.

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CloneID	OSd22243
Clone ID Related Accession (Same CDS sequence)	XM_011677707.1
Accession Version	XM_011677707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-23
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011991989.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTGCT CTTGTGAGAG CAGATATCAT TCGCCTGACC TGAGGAACCT TTGGGAATGT 
CTCGTCCCAG GACCGGTGCC TATTATGGCC GACCAGAGCC CTAGCTGCCA CCAATTAGGG 
GGGTCCAAAC ACCGACGATC AAAGCGAGGT GCCACTGCTA CCACGTCTAC CACTACTAGC 
AACGCCGTGG CCTCCTCGAC CGCTGGATCT TCGGTAGCCA GTCTTGTACA AGACCTATCA 
AATCACCAGA AGCATCAAGT GTCCTCTAGA CAGGAGACTT GCATCAAGTC AAGCAAGAAG 
CAAACTCTCA CCAGTGCAAG CAAGAGGAAG GCTTCAACCG AGAGCCACGG AGCTCCACCT 
TCCAAGGTAT CATCAAGTAC TCTCTTGGAT GCCTCCAACG CTGACAGCAG CAGCGGTGCT 
CAGCTAATCA AAAACAATAG ACACAAACAC GAAGTGTGCA TTCCAGTGAC CACTCTCAAG 
AGCAAGCGTC ACAACCTCCC AAAGAAATCC AAAAAGACTG TCGAGCTTTC CAAGCACAAC 
CTCCTGCAGA GCGAGGACTC TGTCCAGAAC AACGTCAGCG GCAACGAGAG CGCGCACTCG 
CGGGACTCTA AGCAGCGCAG GCGCGGGAGC ACGTCTTCCG CTTCGTCAGC GTGGTCTTCC 
CTCTCTGGAG GATCAAGTCG TTTTAGCTTA TCGGACTCCA CAGGCAGCGT CTGTTTCGCC 
GGGAGTTCTT TCACAAGTGC AGCCCTTCCA ACGGGGGAAC TAGAGGGCTC CGAACCTAGT 
GTGTCGAGTG ATTTGAAAAG TGGCCGGGTG AATATAAACA TTACATCCCG CTCCCTACGC 
TCATACTCCC GACATCACCC CTACCAATTT GAGGCCTTGG ACTACTACTC CCCGCGCCGC 
CTAGGTCTCA GGACTACCAC CACCCTTTCC ACCTCCTTCA CCAAGTCGTT GGAGGAACCC 
AAACTGAACA GTGTTACCTT TGGTGAACCT CAAACCAAAG CAAACGATCT GACCGCAAGT 
GCTACCAAAG CCAAAAGCCT TACGCCAAGC GACCAAGCAG GTTCTAAGAA GGCCAAAGGG 
ACGGTCTCCA AAACTGCAAT ACAGAAAACC CCTGTGCAAG CAGCAGCACA GGCCAAGAGG 
AAAAGCAATA AGAAACTTGC CAAGCCCGCT ACCGCCAAGC AATCTTCATC GGCTTCTGCG 
TCACAGCCAA AAGCCAGAAA GCAGAGTAGC AACCACAAAG CAACTGTCAA AGGGAAGGGA 
AAGGGCCGGT CATCGACCGC TCGCTCCGAT TCTCTCCGTC AGAGGTCAAC AGAAACCACC 
ACGGGAACGT GTGCAAGCAC AAGGAGCCGG CGCAGCTCGG GCATGAGTAA GCGAGGGCCA 
GGGGGCTCTG GCCGACAGGA GGGCAGAACT GGAGGAAGGG ATGCTAATAC TGACGCCAAC 
CCAACTAATG CTCCTACCGC ATCTGACGAG TCAGCACAGG GAGCCACTGC CGCAGGATCC 
AGCACAGGCG CCTCGGCCTT AGCAGCCGGC AGCAAAGGCG ACGCTGACGA GTCTGAAGTT 
ACGAGACTAC AAGCTCTTCT GGAGTCCCGA GGGGTACCTC CACATCTGTT CAACGCTCTT 
GGACCGCGCA TGCACCAGCT TCTACAACGC AGCATGGGGT CTGGAGCTAG CAGCAAAGCT 
CACCAGCTAT TATCAGGCCT GCAGGCCAGC GGAGACGAGA GCCAGCAGCT TCAGGCTGTG 
ATCGAGATGT GCCAGCTCCT GGTGATGGGC AACGAGGACA CGCTGGGGGG ATTCCCTGTC 
AAGCAGGTGG TCCCCGCCCT CATCAGCCTA CTCTCCATGG AACATAACTT TGACATCATG 
AACCATGCCT GCCGCGCATT GACCTACATG ATGGAAGCTC TACCGAGGTC ATCGTCTGTT 
GTCATGGATG CAATACCGGT ATTCCTAGAG AAGCTCCAAG TGATCCAGTG CATGGATGTC 
GCCGAGCAAT CTCTAACGGC TCTGGAGATG CTGTCTCGAA GACACAGCAA AGCCATACTT 
CAGGCAGGTG GTCTCTCTGC ATGCCTTCTC TACATCGAGT TCTTCTCAAT AACTGCCCAG 
CGAAATGCCT TGGCTGTTGC AGCTAACTGT TGCCAAAGCA TGGGACCGGA TGAATTTAAT 
TTGGTTGCCA ACTCGTTACC ATTGCTCACT GGAAGACTTC AACATCAGGA TAAGAAGTCT 
GTAGAGAGCT GTTGCCTGTG TTTCGCCCGT CTCGTGGATA ACTATCAGAA TGATGAAAAG 
CTTCTATTGC TCATAGCAGA ACATGGTCTT CTCACCAACC TACAACAACT GCTGGTAGTG 
ACACCCCCTA TCATCAGTAC AGGGACGTTC ATCATGGTGA TTCGTATGCT ATCACTCATG 
TGTGCAAACT GCCCCAGCCT CGCTGTGCAG CTGCTTAAAC AAAATATTGC AGAAACTATC 
AGCTACCTCC TGATTGGAGC TTCAGACTCT GCCAATGTAG ATATTGAGCT TGTATCAAGG 
AGTCCCCAGG AGCTGTATGA AATCACCTCA CTGATTGGTG AGTTGATGCC TAGGCTTCCC 
CGTGATGGAT TGTTCTCCGT CGATGCCTAC CTCAAGAAGG TACCTAACCA GATCACTGAA 
GCAGCCCTGT GGCAGTGGAG GGACGACAGG GGAATGTGGC ATCCGTATAC TCGCATCGAT 
AACAGGATTG TCGAGGCTGC CCATCAAGCA GGAGAAGACG AGGTGACCCT CTCCACCCTG 
GGGCGGACCT ACACCATAGA CTTTAACGCC ATGCAGCAGA TCAATGAGGA CACTGGCACT 
GCTAGACCGG TCCAGAGAAG GCCTAACCCT GCGGCAGGAG CTGGTTCCTC TTCAGCTTCA 
GCAGTAGCAG CTCCCAAGGA AGATGCACGA GTTGTTTTCA TGAAGGAAGA TGGTGACCAG 
TCAGCTGCAT TCATTAAGTC ACTCTTTGCT GTCCTCTACG AAGTCTACAG CTCATCGGCT 
GGACCTGCAG TCAGGCATAA ATGTTTGAAG GCCTTACTAA GAATGGTGTT CTTTGGCAAG 
CCAGATCTCC TGAAGGATAT GCTGAAGAAT CATGCTGTAT CCAGTCACAT AGCAGCCATG 
CTATCGTCTC AAGACCTCAA GGTTGTCGTA GGCGCCCTTC AGATGTCTGA GATCCTCATG 
CAGAAGCTCC CGGATATCTT CCATATCTAC TTCCGCAGAG AAGGTGTGAT GCATCAAGTG 
AAGAACCTCG CTGAGGCCAC CATCACGGCG GACAACTCTC CTCCCATCAA AGATAAGTTC 
CCCATCTCCA AGGTAACGGC TGCTGACCCC TCCTCCTCCT CCTCGACCAC CCCAGCTTCG 
TCTGCATCCT CTGCCTCCTC GGCGTCATCG GCGTCATCTG CATCTTCGGC AGCCTCGTCC 
GCTTCGTCCA CCCATACCCC GTCACCCCGG GATACCATCA ACATCAATGT GGGCTTAGAT 
TCGGATGGTG TACCTCAAGG ACGTCTGAGC GATGCCCTGA AGCTGAAACG AGCAACCAAG 
CGAGGTCCAG GCCGGAAGAG CAACCGCTAC GCTAGCAACG ATGACTCAAC GCCGTCCCAG 
GGAGGTCATG GCCACCACTA CGAGGCCATC GACACCCCAC CGGGCAGACT GAGTGCGGTA 
TCGGTGCCCA TGCCCCCCTC CTCCAGGGCT CCGGGCAGCG GCAGCGGGGG CAGGACAGGG 
GTCAGCAAGG GCAAAGGCTC TACAGGCAAG ATGGTCAGCG GGTCCACCTC CTCACGTTCT 
TCCTTCCTGG CCAGTCTCAA CCTCTCCCGC TGGGGCAGGG GCGCGTCCAA CTCCCCCTCC 
TCCGCCAACA CCACCCCGAG CAACGCCGCC TTAGGTAGCG ACCACCACCA CCACCACCAT 
CACCATCACC ATCACCACCA CCTGCACACC GCGGCAGGGC GCGAGATGGG CCTGCAGAGG 
GGCGGAGCCG GGGGAGGAAT GGGGTACGGG TCGGACAGGA TACTAGGGCA CGGGAGTGAG 
AACCTCCTAG GGGTGCAGAA GGATGCCAGC GGATCGCAGA AGGAAAAAGT CAGGGCCTGG 
ATCAAGGAAC AAGCATCTCA GTTTATACGT CAGTACTTCA GCCAAACAGA TCACCAGGGA 
GAATCACACC CAGCCCTCAC CTTACTCAAT CAGCTCTGTA CCAATGCAGA CAAACTGACA 
CTAAAGAGTG ATGAAGGCTG CGAAATGCTC CGGAGCATTG CTCACATCCT CACCAATTCG 
GACATATCCG CCTTTGAGAT CCTGCACAGC GGTCTGGTGG TCAAACTCTT GGACTACATG 
ACCAGCAACA GTGACCACAA TGCCGTCCCG AGGGATCTTA GACTGCAACG TTTCCTACAT 
ATATTCCTAG AATGCCCAGC TCCAGGCAGT CCTCCTTTCA CCTCACTGGA GATCCCCGAG 
AGCGCGCCCT TCGCTTCCCT CGTCTACAAG ATCAACGGCT GCATCAGCCA CCTGGAGCAG 
TTCCCGGTCA AGGTTCATGA CCTGCCCGGC GCCGGCGGGT CATCGGGAGG CACCAGGGGT 
TCCCAGGCAC TCAAGTTCTT CAACACCCAT CAGCTCAAGT GCCAGCTGCA GAGGCATCCC 
GAGTGCACCA ACCTGAGGCA GTGGAGGGGC GGACCGGTCA AGATTGACCC GCTGGCCCTG 
GTGCAAGCCA TCGAGAGGTA CCTCATGGTC AGGAACTACG GACGCATGAG ACAGGATGAT 
GAGGATAGTG ATGATGATGC ATCGGACGAT GAGATTGATG AGTCTGTGGC AACGGCCTTC 
TCCAGCTCCA ACACCAACAT CAAGCACAAA CTCCAGTTCT TGATCGGAGA GCAGGTCCTA 
CCGTACAACA TGACAGTCTA CCAGGCCATC AAGCAGTTTG GTAACGCTGC TAGTGCATCG 
GAAGACGATC GGGATACAGA TGATGAGAGC AACCCGCTAG GAAGGGCAGG TATTTGGATC 
AAGACACACA CGATATGGTA CCGACCCGTC CCAGAGGCTG GTGCGCCCTC CACCCAGAAG 
TCATCATCGG CATCGAACAG CAGCACGACC ACCACCACGA CCTCCACCAG GAAAGGCAAG 
GGAGGGAGCT CGTCCAAGTC CTCTTCTAAG GGAAGAAAAC ATGATGAGAT GTGGATGGAT 
GGTGTGTGTC CAGTAATACA GAAGGAGCTC CATAAGTACC TACATAGCAA TCTACCTGGT 
TCCGTCCCTA TCGAAGACCC CTCTTGTGCA GCTATCTCAC TGCTAAGGGT TCTCTACGGT 
CTTAGCAGAC ATTGGAACAA TCTTTATCCG GGAGCTCTAC CGCAGCAGGC AGTAAGTAGC 
AGTGAGGTTG TGAACAGCAA GCTGTCAGCC AAGGCTAACA GACAGCTACA GGATCCTTTA 
GTAATAATGA CTGGCAACCT ACCTTCATGG CTCAACGCAG TGGCACAGGC ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011676009.1
CDS	112..5568
Translation	MICSCESRYHSPDLRNLWECLVPGPVPIMADQSPSCHQLGGSKHRRSKRGATATTSTTTSNAVASSTAGSSVASLVQDLSNHQKHQVSSRQETCIKSSKKQTLTSASKRKASTESHGAPPSKVSSSTLLDASNADSSSGAQLIKNNRHKHEVCIPVTTLKSKRHNLPKKSKKTVELSKHNLLQSEDSVQNNVSGNESAHSRDSKQRRRGSTSSASSAWSSLSGGSSRFSLSDSTGSVCFAGSSFTSAALPTGELEGSEPSVSSDLKSGRVNINITSRSLRSYSRHHPYQFEALDYYSPRRLGLRTTTTLSTSFTKSLEEPKLNSVTFGEPQTKANDLTASATKAKSLTPSDQAGSKKAKGTVSKTAIQKTPVQAAAQAKRKSNKKLAKPATAKQSSSASASQPKARKQSSNHKATVKGKGKGRSSTARSDSLRQRSTETTTGTCASTRSRRSSGMSKRGPGGSGRQEGRTGGRDANTDANPTNAPTASDESAQGATAAGSSTGASALAAGSKGDADESEVTRLQALLESRGVPPHLFNALGPRMHQLLQRSMGSGASSKAHQLLSGLQASGDESQQLQAVIEMCQLLVMGNEDTLGGFPVKQVVPALISLLSMEHNFDIMNHACRALTYMMEALPRSSSVVMDAIPVFLEKLQVIQCMDVAEQSLTALEMLSRRHSKAILQAGGLSACLLYIEFFSITAQRNALAVAANCCQSMGPDEFNLVANSLPLLTGRLQHQDKKSVESCCLCFARLVDNYQNDEKLLLLIAEHGLLTNLQQLLVVTPPIISTGTFIMVIRMLSLMCANCPSLAVQLLKQNIAETISYLLIGASDSANVDIELVSRSPQELYEITSLIGELMPRLPRDGLFSVDAYLKKVPNQITEAALWQWRDDRGMWHPYTRIDNRIVEAAHQAGEDEVTLSTLGRTYTIDFNAMQQINEDTGTARPVQRRPNPAAGAGSSSASAVAAPKEDARVVFMKEDGDQSAAFIKSLFAVLYEVYSSSAGPAVRHKCLKALLRMVFFGKPDLLKDMLKNHAVSSHIAAMLSSQDLKVVVGALQMSEILMQKLPDIFHIYFRREGVMHQVKNLAEATITADNSPPIKDKFPISKVTAADPSSSSSTTPASSASSASSASSASSASSAASSASSTHTPSPRDTININVGLDSDGVPQGRLSDALKLKRATKRGPGRKSNRYASNDDSTPSQGGHGHHYEAIDTPPGRLSAVSVPMPPSSRAPGSGSGGRTGVSKGKGSTGKMVSGSTSSRSSFLASLNLSRWGRGASNSPSSANTTPSNAALGSDHHHHHHHHHHHHHLHTAAGREMGLQRGGAGGGMGYGSDRILGHGSENLLGVQKDASGSQKEKVRAWIKEQASQFIRQYFSQTDHQGESHPALTLLNQLCTNADKLTLKSDEGCEMLRSIAHILTNSDISAFEILHSGLVVKLLDYMTSNSDHNAVPRDLRLQRFLHIFLECPAPGSPPFTSLEIPESAPFASLVYKINGCISHLEQFPVKVHDLPGAGGSSGGTRGSQALKFFNTHQLKCQLQRHPECTNLRQWRGGPVKIDPLALVQAIERYLMVRNYGRMRQDDEDSDDDASDDEIDESVATAFSSSNTNIKHKLQFLIGEQVLPYNMTVYQAIKQFGNAASASEDDRDTDDESNPLGRAGIWIKTHTIWYRPVPEAGAPSTQKSSSASNSSTTTTTTSTRKGKGGSSSKSSSKGRKHDEMWMDGVCPVIQKELHKYLHSNLPGSVPIEDPSCAAISLLRVLYGLSRHWNNLYPGALPQQAVSSSEVVNSKLSAKANRQLQDPLVIMTGNLPSWLNAVAQAW

Target ORF information:

RefSeq Version	XM_011677707.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011677707.1

ORF Insert Sequence:

ATGATTTGCT CTTGTGAGAG CAGATATCAT TCGCCTGACC TGAGGAACCT TTGGGAATGT 
CTCGTCCCAG GACCGGTGCC TATTATGGCC GACCAGAGCC CTAGCTGCCA CCAATTAGGG 
GGGTCCAAAC ACCGACGATC AAAGCGAGGT GCCACTGCTA CCACGTCTAC CACTACTAGC 
AACGCCGTGG CCTCCTCGAC CGCTGGATCT TCGGTAGCCA GTCTTGTACA AGACCTATCA 
AATCACCAGA AGCATCAAGT GTCCTCTAGA CAGGAGACTT GCATCAAGTC AAGCAAGAAG 
CAAACTCTCA CCAGTGCAAG CAAGAGGAAG GCTTCAACCG AGAGCCACGG AGCTCCACCT 
TCCAAGGTAT CATCAAGTAC TCTCTTGGAT GCCTCCAACG CTGACAGCAG CAGCGGTGCT 
CAGCTAATCA AAAACAATAG ACACAAACAC GAAGTGTGCA TTCCAGTGAC CACTCTCAAG 
AGCAAGCGTC ACAACCTCCC AAAGAAATCC AAAAAGACTG TCGAGCTTTC CAAGCACAAC 
CTCCTGCAGA GCGAGGACTC TGTCCAGAAC AACGTCAGCG GCAACGAGAG CGCGCACTCG 
CGGGACTCTA AGCAGCGCAG GCGCGGGAGC ACGTCTTCCG CTTCGTCAGC GTGGTCTTCC 
CTCTCTGGAG GATCAAGTCG TTTTAGCTTA TCGGACTCCA CAGGCAGCGT CTGTTTCGCC 
GGGAGTTCTT TCACAAGTGC AGCCCTTCCA ACGGGGGAAC TAGAGGGCTC CGAACCTAGT 
GTGTCGAGTG ATTTGAAAAG TGGCCGGGTG AATATAAACA TTACATCCCG CTCCCTACGC 
TCATACTCCC GACATCACCC CTACCAATTT GAGGCCTTGG ACTACTACTC CCCGCGCCGC 
CTAGGTCTCA GGACTACCAC CACCCTTTCC ACCTCCTTCA CCAAGTCGTT GGAGGAACCC 
AAACTGAACA GTGTTACCTT TGGTGAACCT CAAACCAAAG CAAACGATCT GACCGCAAGT 
GCTACCAAAG CCAAAAGCCT TACGCCAAGC GACCAAGCAG GTTCTAAGAA GGCCAAAGGG 
ACGGTCTCCA AAACTGCAAT ACAGAAAACC CCTGTGCAAG CAGCAGCACA GGCCAAGAGG 
AAAAGCAATA AGAAACTTGC CAAGCCCGCT ACCGCCAAGC AATCTTCATC GGCTTCTGCG 
TCACAGCCAA AAGCCAGAAA GCAGAGTAGC AACCACAAAG CAACTGTCAA AGGGAAGGGA 
AAGGGCCGGT CATCGACCGC TCGCTCCGAT TCTCTCCGTC AGAGGTCAAC AGAAACCACC 
ACGGGAACGT GTGCAAGCAC AAGGAGCCGG CGCAGCTCGG GCATGAGTAA GCGAGGGCCA 
GGGGGCTCTG GCCGACAGGA GGGCAGAACT GGAGGAAGGG ATGCTAATAC TGACGCCAAC 
CCAACTAATG CTCCTACCGC ATCTGACGAG TCAGCACAGG GAGCCACTGC CGCAGGATCC 
AGCACAGGCG CCTCGGCCTT AGCAGCCGGC AGCAAAGGCG ACGCTGACGA GTCTGAAGTT 
ACGAGACTAC AAGCTCTTCT GGAGTCCCGA GGGGTACCTC CACATCTGTT CAACGCTCTT 
GGACCGCGCA TGCACCAGCT TCTACAACGC AGCATGGGGT CTGGAGCTAG CAGCAAAGCT 
CACCAGCTAT TATCAGGCCT GCAGGCCAGC GGAGACGAGA GCCAGCAGCT TCAGGCTGTG 
ATCGAGATGT GCCAGCTCCT GGTGATGGGC AACGAGGACA CGCTGGGGGG ATTCCCTGTC 
AAGCAGGTGG TCCCCGCCCT CATCAGCCTA CTCTCCATGG AACATAACTT TGACATCATG 
AACCATGCCT GCCGCGCATT GACCTACATG ATGGAAGCTC TACCGAGGTC ATCGTCTGTT 
GTCATGGATG CAATACCGGT ATTCCTAGAG AAGCTCCAAG TGATCCAGTG CATGGATGTC 
GCCGAGCAAT CTCTAACGGC TCTGGAGATG CTGTCTCGAA GACACAGCAA AGCCATACTT 
CAGGCAGGTG GTCTCTCTGC ATGCCTTCTC TACATCGAGT TCTTCTCAAT AACTGCCCAG 
CGAAATGCCT TGGCTGTTGC AGCTAACTGT TGCCAAAGCA TGGGACCGGA TGAATTTAAT 
TTGGTTGCCA ACTCGTTACC ATTGCTCACT GGAAGACTTC AACATCAGGA TAAGAAGTCT 
GTAGAGAGCT GTTGCCTGTG TTTCGCCCGT CTCGTGGATA ACTATCAGAA TGATGAAAAG 
CTTCTATTGC TCATAGCAGA ACATGGTCTT CTCACCAACC TACAACAACT GCTGGTAGTG 
ACACCCCCTA TCATCAGTAC AGGGACGTTC ATCATGGTGA TTCGTATGCT ATCACTCATG 
TGTGCAAACT GCCCCAGCCT CGCTGTGCAG CTGCTTAAAC AAAATATTGC AGAAACTATC 
AGCTACCTCC TGATTGGAGC TTCAGACTCT GCCAATGTAG ATATTGAGCT TGTATCAAGG 
AGTCCCCAGG AGCTGTATGA AATCACCTCA CTGATTGGTG AGTTGATGCC TAGGCTTCCC 
CGTGATGGAT TGTTCTCCGT CGATGCCTAC CTCAAGAAGG TACCTAACCA GATCACTGAA 
GCAGCCCTGT GGCAGTGGAG GGACGACAGG GGAATGTGGC ATCCGTATAC TCGCATCGAT 
AACAGGATTG TCGAGGCTGC CCATCAAGCA GGAGAAGACG AGGTGACCCT CTCCACCCTG 
GGGCGGACCT ACACCATAGA CTTTAACGCC ATGCAGCAGA TCAATGAGGA CACTGGCACT 
GCTAGACCGG TCCAGAGAAG GCCTAACCCT GCGGCAGGAG CTGGTTCCTC TTCAGCTTCA 
GCAGTAGCAG CTCCCAAGGA AGATGCACGA GTTGTTTTCA TGAAGGAAGA TGGTGACCAG 
TCAGCTGCAT TCATTAAGTC ACTCTTTGCT GTCCTCTACG AAGTCTACAG CTCATCGGCT 
GGACCTGCAG TCAGGCATAA ATGTTTGAAG GCCTTACTAA GAATGGTGTT CTTTGGCAAG 
CCAGATCTCC TGAAGGATAT GCTGAAGAAT CATGCTGTAT CCAGTCACAT AGCAGCCATG 
CTATCGTCTC AAGACCTCAA GGTTGTCGTA GGCGCCCTTC AGATGTCTGA GATCCTCATG 
CAGAAGCTCC CGGATATCTT CCATATCTAC TTCCGCAGAG AAGGTGTGAT GCATCAAGTG 
AAGAACCTCG CTGAGGCCAC CATCACGGCG GACAACTCTC CTCCCATCAA AGATAAGTTC 
CCCATCTCCA AGGTAACGGC TGCTGACCCC TCCTCCTCCT CCTCGACCAC CCCAGCTTCG 
TCTGCATCCT CTGCCTCCTC GGCGTCATCG GCGTCATCTG CATCTTCGGC AGCCTCGTCC 
GCTTCGTCCA CCCATACCCC GTCACCCCGG GATACCATCA ACATCAATGT GGGCTTAGAT 
TCGGATGGTG TACCTCAAGG ACGTCTGAGC GATGCCCTGA AGCTGAAACG AGCAACCAAG 
CGAGGTCCAG GCCGGAAGAG CAACCGCTAC GCTAGCAACG ATGACTCAAC GCCGTCCCAG 
GGAGGTCATG GCCACCACTA CGAGGCCATC GACACCCCAC CGGGCAGACT GAGTGCGGTA 
TCGGTGCCCA TGCCCCCCTC CTCCAGGGCT CCGGGCAGCG GCAGCGGGGG CAGGACAGGG 
GTCAGCAAGG GCAAAGGCTC TACAGGCAAG ATGGTCAGCG GGTCCACCTC CTCACGTTCT 
TCCTTCCTGG CCAGTCTCAA CCTCTCCCGC TGGGGCAGGG GCGCGTCCAA CTCCCCCTCC 
TCCGCCAACA CCACCCCGAG CAACGCCGCC TTAGGTAGCG ACCACCACCA CCACCACCAT 
CACCATCACC ATCACCACCA CCTGCACACC GCGGCAGGGC GCGAGATGGG CCTGCAGAGG 
GGCGGAGCCG GGGGAGGAAT GGGGTACGGG TCGGACAGGA TACTAGGGCA CGGGAGTGAG 
AACCTCCTAG GGGTGCAGAA GGATGCCAGC GGATCGCAGA AGGAAAAAGT CAGGGCCTGG 
ATCAAGGAAC AAGCATCTCA GTTTATACGT CAGTACTTCA GCCAAACAGA TCACCAGGGA 
GAATCACACC CAGCCCTCAC CTTACTCAAT CAGCTCTGTA CCAATGCAGA CAAACTGACA 
CTAAAGAGTG ATGAAGGCTG CGAAATGCTC CGGAGCATTG CTCACATCCT CACCAATTCG 
GACATATCCG CCTTTGAGAT CCTGCACAGC GGTCTGGTGG TCAAACTCTT GGACTACATG 
ACCAGCAACA GTGACCACAA TGCCGTCCCG AGGGATCTTA GACTGCAACG TTTCCTACAT 
ATATTCCTAG AATGCCCAGC TCCAGGCAGT CCTCCTTTCA CCTCACTGGA GATCCCCGAG 
AGCGCGCCCT TCGCTTCCCT CGTCTACAAG ATCAACGGCT GCATCAGCCA CCTGGAGCAG 
TTCCCGGTCA AGGTTCATGA CCTGCCCGGC GCCGGCGGGT CATCGGGAGG CACCAGGGGT 
TCCCAGGCAC TCAAGTTCTT CAACACCCAT CAGCTCAAGT GCCAGCTGCA GAGGCATCCC 
GAGTGCACCA ACCTGAGGCA GTGGAGGGGC GGACCGGTCA AGATTGACCC GCTGGCCCTG 
GTGCAAGCCA TCGAGAGGTA CCTCATGGTC AGGAACTACG GACGCATGAG ACAGGATGAT 
GAGGATAGTG ATGATGATGC ATCGGACGAT GAGATTGATG AGTCTGTGGC AACGGCCTTC 
TCCAGCTCCA ACACCAACAT CAAGCACAAA CTCCAGTTCT TGATCGGAGA GCAGGTCCTA 
CCGTACAACA TGACAGTCTA CCAGGCCATC AAGCAGTTTG GTAACGCTGC TAGTGCATCG 
GAAGACGATC GGGATACAGA TGATGAGAGC AACCCGCTAG GAAGGGCAGG TATTTGGATC 
AAGACACACA CGATATGGTA CCGACCCGTC CCAGAGGCTG GTGCGCCCTC CACCCAGAAG 
TCATCATCGG CATCGAACAG CAGCACGACC ACCACCACGA CCTCCACCAG GAAAGGCAAG 
GGAGGGAGCT CGTCCAAGTC CTCTTCTAAG GGAAGAAAAC ATGATGAGAT GTGGATGGAT 
GGTGTGTGTC CAGTAATACA GAAGGAGCTC CATAAGTACC TACATAGCAA TCTACCTGGT 
TCCGTCCCTA TCGAAGACCC CTCTTGTGCA GCTATCTCAC TGCTAAGGGT TCTCTACGGT 
CTTAGCAGAC ATTGGAACAA TCTTTATCCG GGAGCTCTAC CGCAGCAGGC AGTAAGTAGC 
AGTGAGGTTG TGAACAGCAA GCTGTCAGCC AAGGCTAACA GACAGCTACA GGATCCTTTA 
GTAATAATGA CTGGCAACCT ACCTTCATGG CTCAACGCAG TGGCACAGGC ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC578329 ( XM_030981766.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030837626.1 Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), mRNA.

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CloneID	OSd48011
Clone ID Related Accession (Same CDS sequence)	XM_030981766.1
Accession Version	XM_030981766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	E3 ubiquitin-protein ligase TRIP12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145597.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTGCT CTTGTGAGAG CAGATATCAT TCGCCTGACC TGAGGAACCT TTGGGAATGT 
CTCGTCCCAG GACCGGTGCC TATTATGGCC GACCAGAGCC CTAGCTGCCA CCAATTAGGG 
GGGTCCAAAC ACCGACGATC AAAGCGAGGT GCCACTGCTA CCACGTCTAC CACTACTAGC 
AACGCCGTGG CCTCCTCGAC CGCTGGATCT TCGGTAGCCA GTCTTGTACA AGACCTATCA 
AATCACCAGA AGCATCAAGT GTCCTCTAGA CAGGAGACTT GCATCAAGTC AAGCAAGAAG 
CAAACTCTCA CCAGTGCAAG CAAGAGGAAG GCTTCAACCG AGAGCCACGG AGCTCCACCT 
TCCAAGGTAT CATCAAGTAC TCTCTTGGAT GCCTCCAACG CTGACAGCAG CAGCGGTGCT 
CAGCTAATCA AAAACAATAG ACACAAACAC GAAGTGTGCA TTCCAGTGAC CACTCTCAAG 
AGCAAGCGTC ACAACCTCCC AAAGAAATCC AAAAAGACTG TCGAGCTTTC CAAGCACAAC 
CTCCTGCAGA GCGAGGACTC TGTCCAGAAC AACGTCAGCG GCAACGAGAG CGCGCACTCG 
CGGGACTCTA AGCAGCGCAG GCGCGGGAGC ACGTCTTCCG CTTCGTCAGC GTGGTCTTCC 
CTCTCTGGAG GATCAAGTCG TTTTAGCTTA TCGGACTCCA CAGGCAGCGT CTGTTTCGCC 
GGGAGTTCTT TCACAAGTGC AGCCCTTCCA ACGGGGGAAC TAGAGGGCTC CGAACCTAGT 
GTGTCGAGTG ATTTGAAAAG TGGCCGGGTG AATATAAACA TTACATCCCG CTCCCTACGC 
TCATACTCCC GACATCACCC CTACCAATTT GAGGCCTTGG ACTACTACTC CCCGCGCCGC 
CTAGGTCTCA GGACTACCAC CACCCTTTCC ACCTCCTTCA CCAAGTCGTT GGAGGAACCC 
AAACTGAACA GTGTTACCTT TGGTGAACCT CAAACCAAAG CAAACGATCT GACCGCAAGT 
GCTACCAAAG CCAAAAGCCT TACGCCAAGC GACCAAGCAG GTTCTAAGAA GGCCAAAGGG 
ACGGTCTCCA AAACTGCAAT ACAGAAAACC CCTGTGCAAG CAGCAGCACA GGCCAAGAGG 
AAAAGCAATA AGAAACTTGC CAAGCCCGCT ACCGCCAAGC AATCTTCATC GGCTTCTGCG 
TCACAGCCAA AAGCCAGAAA GCAGAGTAGC AACCACAAAG CAACTGTCAA AGGGAAGGGA 
AAGGGCCGGT CATCGACCGC TCGCTCCGAT TCTCTCCGTC AGAGGTCAAC AGAAACCACC 
ACGGGAACGT GTGCAAGCAC AAGGAGCCGG CGCAGCTCGG GCATGAGTAA GCGAGGGCCA 
GGGGGCTCTG GCCGACAGGA GGGCAGAACT GGAGGAAGGG ATGCTAATAC TGACGCCAAC 
CCAACTAATG CTCCTACCGC ATCTGACGAG TCAGCACAGG GAGCCACTGC CGCAGGATCC 
AGCACAGGCG CCTCGGCCTT AGCAGCCGGC AGCAAAGGCG ACGCTGACGA GTCTGAAGTT 
ACGAGACTAC AAGCTCTTCT GGAGTCCCGA GGGGTACCTC CACATCTGTT CAACGCTCTT 
GGACCGCGCA TGCACCAGCT TCTACAACGC AGCATGGGGT CTGGAGCTAG CAGCAAAGCT 
CACCAGCTAT TATCAGGCCT GCAGGCCAGC GGAGACGAGA GCCAGCAGCT TCAGGCTGTG 
ATCGAGATGT GCCAGCTCCT GGTGATGGGC AACGAGGACA CGCTGGGGGG ATTCCCTGTC 
AAGCAGGTGG TCCCCGCCCT CATCAGCCTA CTCTCCATGG AACATAACTT TGACATCATG 
AACCATGCCT GCCGCGCATT GACCTACATG ATGGAAGCTC TACCGAGGTC ATCGTCTGTT 
GTCATGGATG CAATACCGGT ATTCCTAGAG AAGCTCCAAG TGATCCAGTG CATGGATGTC 
GCCGAGCAAT CTCTAACGGC TCTGGAGATG CTGTCTCGAA GACACAGCAA AGCCATACTT 
CAGGCAGGTG GTCTCTCTGC ATGCCTTCTC TACATCGAGT TCTTCTCAAT AACTGCCCAG 
CGAAATGCCT TGGCTGTTGC AGCTAACTGT TGCCAAAGCA TGGGACCGGA TGAATTTAAT 
TTGGTTGCCA ACTCGTTACC ATTGCTCACT GGAAGACTTC AACATCAGGA TAAGAAGTCT 
GTAGAGAGCT GTTGCCTGTG TTTCGCCCGT CTCGTGGATA ACTATCAGAA TGATGAAAAG 
CTTCTATTGC TCATAGCAGA ACATGGTCTT CTCACCAACC TACAACAACT GCTGGTAGTG 
ACACCCCCTA TCATCAGTAC AGGGACGTTC ATCATGGTGA TTCGTATGCT ATCACTCATG 
TGTGCAAACT GCCCCAGCCT CGCTGTGCAG CTGCTTAAAC AAAATATTGC AGAAACTATC 
AGCTACCTCC TGATTGGAGC TTCAGACTCT GCCAATGTAG ATATTGAGCT TGTATCAAGG 
AGTCCCCAGG AGCTGTATGA AATCACCTCA CTGATTGGTG AGTTGATGCC TAGGCTTCCC 
CGTGATGGAT TGTTCTCCGT CGATGCCTAC CTCAAGAAGG TACCTAACCA GATCACTGAA 
GCAGCCCTGT GGCAGTGGAG GGACGACAGG GGAATGTGGC ATCCGTATAC TCGCATCGAT 
AACAGGATTG TCGAGGCTGC CCATCAAGCA GGAGAAGACG AGGTGACCCT CTCCACCCTG 
GGGCGGACCT ACACCATAGA CTTTAACGCC ATGCAGCAGA TCAATGAGGA CACTGGCACT 
GCTAGACCGG TCCAGAGAAG GCCTAACCCT GCGGCAGGAG CTGGTTCCTC TTCAGCTTCA 
GCAGTAGCAG CTCCCAAGGA AGATGCACGA GTTGTTTTCA TGAAGGAAGA TGGTGACCAG 
TCAGCTGCAT TCATTAAGTC ACTCTTTGCT GTCCTCTACG AAGTCTACAG CTCATCGGCT 
GGACCTGCAG TCAGGCATAA ATGTTTGAAG GCCTTACTAA GAATGGTGTT CTTTGGCAAG 
CCAGATCTCC TGAAGGATAT GCTGAAGAAT CATGCTGTAT CCAGTCACAT AGCAGCCATG 
CTATCGTCTC AAGACCTCAA GGTTGTCGTA GGCGCCCTTC AGATGTCTGA GATCCTCATG 
CAGAAGCTCC CGGATATCTT CCATATCTAC TTCCGCAGAG AAGGTGTGAT GCATCAAGTG 
AAGAACCTCG CTGAGGCCAC CATCACGGCG GACAACTCTC CTCCCATCAA AGATAAGTTC 
CCCATCTCCA AGGTAACGGC TGCTGACCCC TCCTCCTCCT CCTCGACCAC CCCAGCTTCG 
TCTGCATCCT CTGCCTCCTC GGCGTCATCG GCGTCATCTG CATCTTCGGC AGCCTCGTCC 
GCTTCGTCCA CCCATACCCC GTCACCCCGG GATACCATCA ACATCAATGT GGGCTTAGAT 
TCGGATGGTG TACCTCAAGG ACGTCTGAGC GATGCCCTGA AGCTGAAACG AGCAACCAAG 
CGAGGTCCAG GCCGGAAGAG CAACCGCTAC GCTAGCAACG ATGACTCAAC GCCGTCCCAG 
GGAGGTCATG GCCACCACTA CGAGGCCATC GACACCCCAC CGGGCAGACT GAGTGCGGTA 
TCGGTGCCCA TGCCCCCCTC CTCCAGGGCT CCGGGCAGCG GCAGCGGGGG CAGGACAGGG 
GTCAGCAAGG GCAAAGGCTC TACAGGCAAG ATGGTCAGCG GGTCCACCTC CTCACGTTCT 
TCCTTCCTGG CCAGTCTCAA CCTCTCCCGC TGGGGCAGGG GCGCGTCCAA CTCCCCCTCC 
TCCGCCAACA CCACCCCGAG CAACGCCGCC TTAGGTAGCG ACCACCACCA CCACCACCAT 
CACCATCACC ATCACCACCA CCTGCACACC GCGGCAGGGC GCGAGATGGG CCTGCAGAGG 
GGCGGAGCCG GGGGAGGAAT GGGGTACGGG TCGGACAGGA TACTAGGGCA CGGGAGTGAG 
AACCTCCTAG GGGTGCAGAA GGATGCCAGC GGATCGCAGA AGGAAAAAGT CAGGGCCTGG 
ATCAAGGAAC AAGCATCTCA GTTTATACGT CAGTACTTCA GCCAAACAGA TCACCAGGGA 
GAATCACACC CAGCCCTCAC CTTACTCAAT CAGCTCTGTA CCAATGCAGA CAAACTGACA 
CTAAAGAGTG ATGAAGGCTG CGAAATGCTC CGGAGCATTG CTCACATCCT CACCAATTCG 
GACATATCCG CCTTTGAGAT CCTGCACAGC GGTCTGGTGG TCAAACTCTT GGACTACATG 
ACCAGCAACA GTGACCACAA TGCCGTCCCG AGGGATCTTA GACTGCAACG TTTCCTACAT 
ATATTCCTAG AATGCCCAGC TCCAGGCAGT CCTCCTTTCA CCTCACTGGA GATCCCCGAG 
AGCGCGCCCT TCGCTTCCCT CGTCTACAAG ATCAACGGCT GCATCAGCCA CCTGGAGCAG 
TTCCCGGTCA AGGTTCATGA CCTGCCCGGC GCCGGCGGGT CATCGGGAGG CACCAGGGGT 
TCCCAGGCAC TCAAGTTCTT CAACACCCAT CAGCTCAAGT GCCAGCTGCA GAGGCATCCC 
GAGTGCACCA ACCTGAGGCA GTGGAGGGGC GGACCGGTCA AGATTGACCC GCTGGCCCTG 
GTGCAAGCCA TCGAGAGGTA CCTCATGGTC AGGAACTACG GACGCATGAG ACAGGATGAT 
GAGGATAGTG ATGATGATGC ATCGGACGAT GAGATTGATG AGTCTGTGGC AACGGCCTTC 
TCCAGCTCCA ACACCAACAT CAAGCACAAA CTCCAGTTCT TGATCGGAGA GCAGGTCCTA 
CCGTACAACA TGACAGTCTA CCAGGCCATC AAGCAGTTTG GTAACGCTGC TAGTGCATCG 
GAAGACGATC GGGATACAGA TGATGAGAGC AACCCGCTAG GAAGGGCAGG TATTTGGATC 
AAGACACACA CGATATGGTA CCGACCCGTC CCAGAGGCTG GTGCGCCCTC CACCCAGAAG 
TCATCATCGG CATCGAACAG CAGCACGACC ACCACCACGA CCTCCACCAG GAAAGGCAAG 
GGAGGGAGCT CGTCCAAGTC CTCTTCTAAG GGAAGAAAAC ATGATGAGAT GTGGATGGAT 
GGTGTGTGTC CAGTAATACA GAAGGAGCTC CATAAGTACC TACATAGCAA TCTACCTGGT 
TCCGTCCCTA TCGAAGACCC CTCTTGTGCA GCTATCTCAC TGCTAAGGGT TCTCTACGGT 
CTTAGCAGAC ATTGGAACAA TCTTTATCCG GGAGCTCTAC CGCAGCAGGC AGTAAGTAGC 
AGTGAGGTTG TGAACAGCAA GCTGTCAGCC AAGGCTAACA GACAGCTACA GGATCCTTTA 
GTAATAATGA CTGGCAACCT ACCTTCATGG CTCAACGCAG TGGCACAGGC ATGTCCCTTC 
CTGCTGCCGT TCGATACCCG TCAGCTCCTG TTCTACACAA CAGCGTTTGA CAGAGATCGA 
GCCATGCAGA GGCTACAGGA CAACAACCCA GACATGTCCA CATCAGAGAA TAGTGAGAGG 
GTTGCTCCTA GATTGGACAG AAGGAAAAGA AGTGTATCAA GAGCAGATCT GTTACGACAA 
GCCGAGTCGG TCATGGAGGA TCTAGCTAAC TCCAGGGCAG TGCTGGAAAT ACAGTACGAA 
GATGAGGTTG GTACTGGTTT AGGTCCGACC CTCGAGTTCT ACGCCCTGGT ATCAAGAGAG 
GTACAGCGAG CTGATCTCGA TCTCTGGAGA GGAGAATCGG TTCTTTTACC AGATCCTAAA 
GGCAAGCAAG CTGGAGTGAA GTACATCCAT TCGCCCCACG GTCTCTTCCC ATCCCCGATG 
GGACGGAACG CCAAGGCTTC CGTAGTCGCT AAGATCAAGT CCAAATTTAC CTTCATGGGA 
CGTTTCATTG CAAAGGCACT CATGGACTCT AGAATGATTG ACATTCCACT TAGCCTGCCA 
TTTTTCAAGT GGCTCCTGGG GACGGAGGCT TCGCTGACCT CGTCTGACCT TCAGTTCATT 
GACCGCACCT TGGCCCGATC CTTCCAACAG CTTGAGGAGC TTGGTCAGCA GAAACAACGG 
CTGGTCAATG ACCCCTCTCA GACTGCTGCT AGTCTCCAGC TCGCCCTGGA GTCATTGAGC 
ATAGACGGTA GCCCAGTAGA GGAGCTAGGG CTAGACTTCA CGCTGCCGGG CTATCCTCAT 
ATAGAACTGA AGAAAGGAGG CAAGGACATT GATGTCACCA TTCATAATAT AGATGAGTAC 
CTCAAGCTTG TTGTTCGTTG GACCCTGAAC GAGGGCGTAT CCAGGCAGTT TGAAGCTCTT 
AAAGATGGAT TCCAGTCCGT GTTCCCTCTC TCTCATCTGC AAAGCTTCTA CGGAGAAGAG 
TTGGATCAGC TGTTCTGTGG GAATCGTAGT GAGCCTTGGG ATATCAAGAC GTTACTAGAA 
TGCTGCAGGC CTGATCACGG CTATACTCAT GATAGTCGTG CTGTGAAGCT GTTATTTGAG 
CTCATGGCGT CGTACACAGA CAGTCAACAG AGGGAGTTTC TTCAGTTCGT CACTGGAAGC 
CCCAAACTCC CTGTAGGAGG TTTCCGTTCC CTGAATCCTC CTCTGACGAT CGTACGTAAG 
ATGTTCGAAG GCTCCGAGGA CCCGGACAAA TTCCTTCCCT CTGTGATGAC TTGTGTGAAC 
TACCTGAAGC TCCCTGACTA TACCTCCATG GAAGTCATGG CGTCCAAGCT TTCTATAGCC 
ACGCGAGAAG GACAACAGTC TTTCCATTTA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030837626.1
CDS	194..7009
Translation	MICSCESRYHSPDLRNLWECLVPGPVPIMADQSPSCHQLGGSKHRRSKRGATATTSTTTSNAVASSTAGSSVASLVQDLSNHQKHQVSSRQETCIKSSKKQTLTSASKRKASTESHGAPPSKVSSSTLLDASNADSSSGAQLIKNNRHKHEVCIPVTTLKSKRHNLPKKSKKTVELSKHNLLQSEDSVQNNVSGNESAHSRDSKQRRRGSTSSASSAWSSLSGGSSRFSLSDSTGSVCFAGSSFTSAALPTGELEGSEPSVSSDLKSGRVNINITSRSLRSYSRHHPYQFEALDYYSPRRLGLRTTTTLSTSFTKSLEEPKLNSVTFGEPQTKANDLTASATKAKSLTPSDQAGSKKAKGTVSKTAIQKTPVQAAAQAKRKSNKKLAKPATAKQSSSASASQPKARKQSSNHKATVKGKGKGRSSTARSDSLRQRSTETTTGTCASTRSRRSSGMSKRGPGGSGRQEGRTGGRDANTDANPTNAPTASDESAQGATAAGSSTGASALAAGSKGDADESEVTRLQALLESRGVPPHLFNALGPRMHQLLQRSMGSGASSKAHQLLSGLQASGDESQQLQAVIEMCQLLVMGNEDTLGGFPVKQVVPALISLLSMEHNFDIMNHACRALTYMMEALPRSSSVVMDAIPVFLEKLQVIQCMDVAEQSLTALEMLSRRHSKAILQAGGLSACLLYIEFFSITAQRNALAVAANCCQSMGPDEFNLVANSLPLLTGRLQHQDKKSVESCCLCFARLVDNYQNDEKLLLLIAEHGLLTNLQQLLVVTPPIISTGTFIMVIRMLSLMCANCPSLAVQLLKQNIAETISYLLIGASDSANVDIELVSRSPQELYEITSLIGELMPRLPRDGLFSVDAYLKKVPNQITEAALWQWRDDRGMWHPYTRIDNRIVEAAHQAGEDEVTLSTLGRTYTIDFNAMQQINEDTGTARPVQRRPNPAAGAGSSSASAVAAPKEDARVVFMKEDGDQSAAFIKSLFAVLYEVYSSSAGPAVRHKCLKALLRMVFFGKPDLLKDMLKNHAVSSHIAAMLSSQDLKVVVGALQMSEILMQKLPDIFHIYFRREGVMHQVKNLAEATITADNSPPIKDKFPISKVTAADPSSSSSTTPASSASSASSASSASSASSAASSASSTHTPSPRDTININVGLDSDGVPQGRLSDALKLKRATKRGPGRKSNRYASNDDSTPSQGGHGHHYEAIDTPPGRLSAVSVPMPPSSRAPGSGSGGRTGVSKGKGSTGKMVSGSTSSRSSFLASLNLSRWGRGASNSPSSANTTPSNAALGSDHHHHHHHHHHHHHLHTAAGREMGLQRGGAGGGMGYGSDRILGHGSENLLGVQKDASGSQKEKVRAWIKEQASQFIRQYFSQTDHQGESHPALTLLNQLCTNADKLTLKSDEGCEMLRSIAHILTNSDISAFEILHSGLVVKLLDYMTSNSDHNAVPRDLRLQRFLHIFLECPAPGSPPFTSLEIPESAPFASLVYKINGCISHLEQFPVKVHDLPGAGGSSGGTRGSQALKFFNTHQLKCQLQRHPECTNLRQWRGGPVKIDPLALVQAIERYLMVRNYGRMRQDDEDSDDDASDDEIDESVATAFSSSNTNIKHKLQFLIGEQVLPYNMTVYQAIKQFGNAASASEDDRDTDDESNPLGRAGIWIKTHTIWYRPVPEAGAPSTQKSSSASNSSTTTTTTSTRKGKGGSSSKSSSKGRKHDEMWMDGVCPVIQKELHKYLHSNLPGSVPIEDPSCAAISLLRVLYGLSRHWNNLYPGALPQQAVSSSEVVNSKLSAKANRQLQDPLVIMTGNLPSWLNAVAQACPFLLPFDTRQLLFYTTAFDRDRAMQRLQDNNPDMSTSENSERVAPRLDRRKRSVSRADLLRQAESVMEDLANSRAVLEIQYEDEVGTGLGPTLEFYALVSREVQRADLDLWRGESVLLPDPKGKQAGVKYIHSPHGLFPSPMGRNAKASVVAKIKSKFTFMGRFIAKALMDSRMIDIPLSLPFFKWLLGTEASLTSSDLQFIDRTLARSFQQLEELGQQKQRLVNDPSQTAASLQLALESLSIDGSPVEELGLDFTLPGYPHIELKKGGKDIDVTIHNIDEYLKLVVRWTLNEGVSRQFEALKDGFQSVFPLSHLQSFYGEELDQLFCGNRSEPWDIKTLLECCRPDHGYTHDSRAVKLLFELMASYTDSQQREFLQFVTGSPKLPVGGFRSLNPPLTIVRKMFEGSEDPDKFLPSVMTCVNYLKLPDYTSMEVMASKLSIATREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030981766.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030981766.1

ORF Insert Sequence:

ATGATTTGCT CTTGTGAGAG CAGATATCAT TCGCCTGACC TGAGGAACCT TTGGGAATGT 
CTCGTCCCAG GACCGGTGCC TATTATGGCC GACCAGAGCC CTAGCTGCCA CCAATTAGGG 
GGGTCCAAAC ACCGACGATC AAAGCGAGGT GCCACTGCTA CCACGTCTAC CACTACTAGC 
AACGCCGTGG CCTCCTCGAC CGCTGGATCT TCGGTAGCCA GTCTTGTACA AGACCTATCA 
AATCACCAGA AGCATCAAGT GTCCTCTAGA CAGGAGACTT GCATCAAGTC AAGCAAGAAG 
CAAACTCTCA CCAGTGCAAG CAAGAGGAAG GCTTCAACCG AGAGCCACGG AGCTCCACCT 
TCCAAGGTAT CATCAAGTAC TCTCTTGGAT GCCTCCAACG CTGACAGCAG CAGCGGTGCT 
CAGCTAATCA AAAACAATAG ACACAAACAC GAAGTGTGCA TTCCAGTGAC CACTCTCAAG 
AGCAAGCGTC ACAACCTCCC AAAGAAATCC AAAAAGACTG TCGAGCTTTC CAAGCACAAC 
CTCCTGCAGA GCGAGGACTC TGTCCAGAAC AACGTCAGCG GCAACGAGAG CGCGCACTCG 
CGGGACTCTA AGCAGCGCAG GCGCGGGAGC ACGTCTTCCG CTTCGTCAGC GTGGTCTTCC 
CTCTCTGGAG GATCAAGTCG TTTTAGCTTA TCGGACTCCA CAGGCAGCGT CTGTTTCGCC 
GGGAGTTCTT TCACAAGTGC AGCCCTTCCA ACGGGGGAAC TAGAGGGCTC CGAACCTAGT 
GTGTCGAGTG ATTTGAAAAG TGGCCGGGTG AATATAAACA TTACATCCCG CTCCCTACGC 
TCATACTCCC GACATCACCC CTACCAATTT GAGGCCTTGG ACTACTACTC CCCGCGCCGC 
CTAGGTCTCA GGACTACCAC CACCCTTTCC ACCTCCTTCA CCAAGTCGTT GGAGGAACCC 
AAACTGAACA GTGTTACCTT TGGTGAACCT CAAACCAAAG CAAACGATCT GACCGCAAGT 
GCTACCAAAG CCAAAAGCCT TACGCCAAGC GACCAAGCAG GTTCTAAGAA GGCCAAAGGG 
ACGGTCTCCA AAACTGCAAT ACAGAAAACC CCTGTGCAAG CAGCAGCACA GGCCAAGAGG 
AAAAGCAATA AGAAACTTGC CAAGCCCGCT ACCGCCAAGC AATCTTCATC GGCTTCTGCG 
TCACAGCCAA AAGCCAGAAA GCAGAGTAGC AACCACAAAG CAACTGTCAA AGGGAAGGGA 
AAGGGCCGGT CATCGACCGC TCGCTCCGAT TCTCTCCGTC AGAGGTCAAC AGAAACCACC 
ACGGGAACGT GTGCAAGCAC AAGGAGCCGG CGCAGCTCGG GCATGAGTAA GCGAGGGCCA 
GGGGGCTCTG GCCGACAGGA GGGCAGAACT GGAGGAAGGG ATGCTAATAC TGACGCCAAC 
CCAACTAATG CTCCTACCGC ATCTGACGAG TCAGCACAGG GAGCCACTGC CGCAGGATCC 
AGCACAGGCG CCTCGGCCTT AGCAGCCGGC AGCAAAGGCG ACGCTGACGA GTCTGAAGTT 
ACGAGACTAC AAGCTCTTCT GGAGTCCCGA GGGGTACCTC CACATCTGTT CAACGCTCTT 
GGACCGCGCA TGCACCAGCT TCTACAACGC AGCATGGGGT CTGGAGCTAG CAGCAAAGCT 
CACCAGCTAT TATCAGGCCT GCAGGCCAGC GGAGACGAGA GCCAGCAGCT TCAGGCTGTG 
ATCGAGATGT GCCAGCTCCT GGTGATGGGC AACGAGGACA CGCTGGGGGG ATTCCCTGTC 
AAGCAGGTGG TCCCCGCCCT CATCAGCCTA CTCTCCATGG AACATAACTT TGACATCATG 
AACCATGCCT GCCGCGCATT GACCTACATG ATGGAAGCTC TACCGAGGTC ATCGTCTGTT 
GTCATGGATG CAATACCGGT ATTCCTAGAG AAGCTCCAAG TGATCCAGTG CATGGATGTC 
GCCGAGCAAT CTCTAACGGC TCTGGAGATG CTGTCTCGAA GACACAGCAA AGCCATACTT 
CAGGCAGGTG GTCTCTCTGC ATGCCTTCTC TACATCGAGT TCTTCTCAAT AACTGCCCAG 
CGAAATGCCT TGGCTGTTGC AGCTAACTGT TGCCAAAGCA TGGGACCGGA TGAATTTAAT 
TTGGTTGCCA ACTCGTTACC ATTGCTCACT GGAAGACTTC AACATCAGGA TAAGAAGTCT 
GTAGAGAGCT GTTGCCTGTG TTTCGCCCGT CTCGTGGATA ACTATCAGAA TGATGAAAAG 
CTTCTATTGC TCATAGCAGA ACATGGTCTT CTCACCAACC TACAACAACT GCTGGTAGTG 
ACACCCCCTA TCATCAGTAC AGGGACGTTC ATCATGGTGA TTCGTATGCT ATCACTCATG 
TGTGCAAACT GCCCCAGCCT CGCTGTGCAG CTGCTTAAAC AAAATATTGC AGAAACTATC 
AGCTACCTCC TGATTGGAGC TTCAGACTCT GCCAATGTAG ATATTGAGCT TGTATCAAGG 
AGTCCCCAGG AGCTGTATGA AATCACCTCA CTGATTGGTG AGTTGATGCC TAGGCTTCCC 
CGTGATGGAT TGTTCTCCGT CGATGCCTAC CTCAAGAAGG TACCTAACCA GATCACTGAA 
GCAGCCCTGT GGCAGTGGAG GGACGACAGG GGAATGTGGC ATCCGTATAC TCGCATCGAT 
AACAGGATTG TCGAGGCTGC CCATCAAGCA GGAGAAGACG AGGTGACCCT CTCCACCCTG 
GGGCGGACCT ACACCATAGA CTTTAACGCC ATGCAGCAGA TCAATGAGGA CACTGGCACT 
GCTAGACCGG TCCAGAGAAG GCCTAACCCT GCGGCAGGAG CTGGTTCCTC TTCAGCTTCA 
GCAGTAGCAG CTCCCAAGGA AGATGCACGA GTTGTTTTCA TGAAGGAAGA TGGTGACCAG 
TCAGCTGCAT TCATTAAGTC ACTCTTTGCT GTCCTCTACG AAGTCTACAG CTCATCGGCT 
GGACCTGCAG TCAGGCATAA ATGTTTGAAG GCCTTACTAA GAATGGTGTT CTTTGGCAAG 
CCAGATCTCC TGAAGGATAT GCTGAAGAAT CATGCTGTAT CCAGTCACAT AGCAGCCATG 
CTATCGTCTC AAGACCTCAA GGTTGTCGTA GGCGCCCTTC AGATGTCTGA GATCCTCATG 
CAGAAGCTCC CGGATATCTT CCATATCTAC TTCCGCAGAG AAGGTGTGAT GCATCAAGTG 
AAGAACCTCG CTGAGGCCAC CATCACGGCG GACAACTCTC CTCCCATCAA AGATAAGTTC 
CCCATCTCCA AGGTAACGGC TGCTGACCCC TCCTCCTCCT CCTCGACCAC CCCAGCTTCG 
TCTGCATCCT CTGCCTCCTC GGCGTCATCG GCGTCATCTG CATCTTCGGC AGCCTCGTCC 
GCTTCGTCCA CCCATACCCC GTCACCCCGG GATACCATCA ACATCAATGT GGGCTTAGAT 
TCGGATGGTG TACCTCAAGG ACGTCTGAGC GATGCCCTGA AGCTGAAACG AGCAACCAAG 
CGAGGTCCAG GCCGGAAGAG CAACCGCTAC GCTAGCAACG ATGACTCAAC GCCGTCCCAG 
GGAGGTCATG GCCACCACTA CGAGGCCATC GACACCCCAC CGGGCAGACT GAGTGCGGTA 
TCGGTGCCCA TGCCCCCCTC CTCCAGGGCT CCGGGCAGCG GCAGCGGGGG CAGGACAGGG 
GTCAGCAAGG GCAAAGGCTC TACAGGCAAG ATGGTCAGCG GGTCCACCTC CTCACGTTCT 
TCCTTCCTGG CCAGTCTCAA CCTCTCCCGC TGGGGCAGGG GCGCGTCCAA CTCCCCCTCC 
TCCGCCAACA CCACCCCGAG CAACGCCGCC TTAGGTAGCG ACCACCACCA CCACCACCAT 
CACCATCACC ATCACCACCA CCTGCACACC GCGGCAGGGC GCGAGATGGG CCTGCAGAGG 
GGCGGAGCCG GGGGAGGAAT GGGGTACGGG TCGGACAGGA TACTAGGGCA CGGGAGTGAG 
AACCTCCTAG GGGTGCAGAA GGATGCCAGC GGATCGCAGA AGGAAAAAGT CAGGGCCTGG 
ATCAAGGAAC AAGCATCTCA GTTTATACGT CAGTACTTCA GCCAAACAGA TCACCAGGGA 
GAATCACACC CAGCCCTCAC CTTACTCAAT CAGCTCTGTA CCAATGCAGA CAAACTGACA 
CTAAAGAGTG ATGAAGGCTG CGAAATGCTC CGGAGCATTG CTCACATCCT CACCAATTCG 
GACATATCCG CCTTTGAGAT CCTGCACAGC GGTCTGGTGG TCAAACTCTT GGACTACATG 
ACCAGCAACA GTGACCACAA TGCCGTCCCG AGGGATCTTA GACTGCAACG TTTCCTACAT 
ATATTCCTAG AATGCCCAGC TCCAGGCAGT CCTCCTTTCA CCTCACTGGA GATCCCCGAG 
AGCGCGCCCT TCGCTTCCCT CGTCTACAAG ATCAACGGCT GCATCAGCCA CCTGGAGCAG 
TTCCCGGTCA AGGTTCATGA CCTGCCCGGC GCCGGCGGGT CATCGGGAGG CACCAGGGGT 
TCCCAGGCAC TCAAGTTCTT CAACACCCAT CAGCTCAAGT GCCAGCTGCA GAGGCATCCC 
GAGTGCACCA ACCTGAGGCA GTGGAGGGGC GGACCGGTCA AGATTGACCC GCTGGCCCTG 
GTGCAAGCCA TCGAGAGGTA CCTCATGGTC AGGAACTACG GACGCATGAG ACAGGATGAT 
GAGGATAGTG ATGATGATGC ATCGGACGAT GAGATTGATG AGTCTGTGGC AACGGCCTTC 
TCCAGCTCCA ACACCAACAT CAAGCACAAA CTCCAGTTCT TGATCGGAGA GCAGGTCCTA 
CCGTACAACA TGACAGTCTA CCAGGCCATC AAGCAGTTTG GTAACGCTGC TAGTGCATCG 
GAAGACGATC GGGATACAGA TGATGAGAGC AACCCGCTAG GAAGGGCAGG TATTTGGATC 
AAGACACACA CGATATGGTA CCGACCCGTC CCAGAGGCTG GTGCGCCCTC CACCCAGAAG 
TCATCATCGG CATCGAACAG CAGCACGACC ACCACCACGA CCTCCACCAG GAAAGGCAAG 
GGAGGGAGCT CGTCCAAGTC CTCTTCTAAG GGAAGAAAAC ATGATGAGAT GTGGATGGAT 
GGTGTGTGTC CAGTAATACA GAAGGAGCTC CATAAGTACC TACATAGCAA TCTACCTGGT 
TCCGTCCCTA TCGAAGACCC CTCTTGTGCA GCTATCTCAC TGCTAAGGGT TCTCTACGGT 
CTTAGCAGAC ATTGGAACAA TCTTTATCCG GGAGCTCTAC CGCAGCAGGC AGTAAGTAGC 
AGTGAGGTTG TGAACAGCAA GCTGTCAGCC AAGGCTAACA GACAGCTACA GGATCCTTTA 
GTAATAATGA CTGGCAACCT ACCTTCATGG CTCAACGCAG TGGCACAGGC ATGTCCCTTC 
CTGCTGCCGT TCGATACCCG TCAGCTCCTG TTCTACACAA CAGCGTTTGA CAGAGATCGA 
GCCATGCAGA GGCTACAGGA CAACAACCCA GACATGTCCA CATCAGAGAA TAGTGAGAGG 
GTTGCTCCTA GATTGGACAG AAGGAAAAGA AGTGTATCAA GAGCAGATCT GTTACGACAA 
GCCGAGTCGG TCATGGAGGA TCTAGCTAAC TCCAGGGCAG TGCTGGAAAT ACAGTACGAA 
GATGAGGTTG GTACTGGTTT AGGTCCGACC CTCGAGTTCT ACGCCCTGGT ATCAAGAGAG 
GTACAGCGAG CTGATCTCGA TCTCTGGAGA GGAGAATCGG TTCTTTTACC AGATCCTAAA 
GGCAAGCAAG CTGGAGTGAA GTACATCCAT TCGCCCCACG GTCTCTTCCC ATCCCCGATG 
GGACGGAACG CCAAGGCTTC CGTAGTCGCT AAGATCAAGT CCAAATTTAC CTTCATGGGA 
CGTTTCATTG CAAAGGCACT CATGGACTCT AGAATGATTG ACATTCCACT TAGCCTGCCA 
TTTTTCAAGT GGCTCCTGGG GACGGAGGCT TCGCTGACCT CGTCTGACCT TCAGTTCATT 
GACCGCACCT TGGCCCGATC CTTCCAACAG CTTGAGGAGC TTGGTCAGCA GAAACAACGG 
CTGGTCAATG ACCCCTCTCA GACTGCTGCT AGTCTCCAGC TCGCCCTGGA GTCATTGAGC 
ATAGACGGTA GCCCAGTAGA GGAGCTAGGG CTAGACTTCA CGCTGCCGGG CTATCCTCAT 
ATAGAACTGA AGAAAGGAGG CAAGGACATT GATGTCACCA TTCATAATAT AGATGAGTAC 
CTCAAGCTTG TTGTTCGTTG GACCCTGAAC GAGGGCGTAT CCAGGCAGTT TGAAGCTCTT 
AAAGATGGAT TCCAGTCCGT GTTCCCTCTC TCTCATCTGC AAAGCTTCTA CGGAGAAGAG 
TTGGATCAGC TGTTCTGTGG GAATCGTAGT GAGCCTTGGG ATATCAAGAC GTTACTAGAA 
TGCTGCAGGC CTGATCACGG CTATACTCAT GATAGTCGTG CTGTGAAGCT GTTATTTGAG 
CTCATGGCGT CGTACACAGA CAGTCAACAG AGGGAGTTTC TTCAGTTCGT CACTGGAAGC 
CCCAAACTCC CTGTAGGAGG TTTCCGTTCC CTGAATCCTC CTCTGACGAT CGTACGTAAG 
ATGTTCGAAG GCTCCGAGGA CCCGGACAAA TTCCTTCCCT CTGTGATGAC TTGTGTGAAC 
TACCTGAAGC TCCCTGACTA TACCTCCATG GAAGTCATGG CGTCCAAGCT TTCTATAGCC 
ACGCGAGAAG GACAACAGTC TTTCCATTTA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC578329 ( XM_011677706.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011676008.1 Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), transcript variant X1, mRNA.

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CloneID	OSd22242
Clone ID Related Accession (Same CDS sequence)	XM_011677706.1
Accession Version	XM_011677706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-23
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011991989.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTGCT CTTGTGAGAG CAGATATCAT TCGCCTGACC TGAGGAACCT TTGGGAATGT 
CTCGTCCCAG GACCGGTGCC TATTATGGCC GACCAGAGCC CTAGCTGCCA CCAATTAGGG 
GGGTCCAAAC ACCGACGATC AAAGCGAGGT GCCACTGCTA CCACGTCTAC CACTACTAGC 
AACGCCGTGG CCTCCTCGAC CGCTGGATCT TCGGTAGCCA GTCTTGTACA AGACCTATCA 
AATCACCAGA AGCATCAAGT GTCCTCTAGA CAGGAGACTT GCATCAAGTC AAGCAAGAAG 
CAAACTCTCA CCAGTGCAAG CAAGAGGAAG GCTTCAACCG AGAGCCACGG AGCTCCACCT 
TCCAAGGTAT CATCAAGTAC TCTCTTGGAT GCCTCCAACG CTGACAGCAG CAGCGGTGCT 
CAGCTAATCA AAAACAATAG ACACAAACAC GAAGTGTGCA TTCCAGTGAC CACTCTCAAG 
AGCAAGCGTC ACAACCTCCC AAAGAAATCC AAAAAGACTG TCGAGCTTTC CAAGCACAAC 
CTCCTGCAGA GCGAGGACTC TGTCCAGAAC AACGTCAGCG GCAACGAGAG CGCGCACTCG 
CGGGACTCTA AGCAGCGCAG GCGCGGGAGC ACGTCTTCCG CTTCGTCAGC GTGGTCTTCC 
CTCTCTGGAG GATCAAGTCG TTTTAGCTTA TCGGACTCCA CAGGCAGCGT CTGTTTCGCC 
GGGAGTTCTT TCACAAGTGC AGCCCTTCCA ACGGGGGAAC TAGAGGGCTC CGAACCTAGT 
GTGTCGAGTG ATTTGAAAAG TGGCCGGGTG AATATAAACA TTACATCCCG CTCCCTACGC 
TCATACTCCC GACATCACCC CTACCAATTT GAGGCCTTGG ACTACTACTC CCCGCGCCGC 
CTAGGTCTCA GGACTACCAC CACCCTTTCC ACCTCCTTCA CCAAGTCGTT GGAGGAACCC 
AAACTGAACA GTGTTACCTT TGGTGAACCT CAAACCAAAG CAAACGATCT GACCGCAAGT 
GCTACCAAAG CCAAAAGCCT TACGCCAAGC GACCAAGCAG GTTCTAAGAA GGCCAAAGGG 
ACGGTCTCCA AAACTGCAAT ACAGAAAACC CCTGTGCAAG CAGCAGCACA GGCCAAGAGG 
AAAAGCAATA AGAAACTTGC CAAGCCCGCT ACCGCCAAGC AATCTTCATC GGCTTCTGCG 
TCACAGCCAA AAGCCAGAAA GCAGAGTAGC AACCACAAAG CAACTGTCAA AGGGAAGGGA 
AAGGGCCGGT CATCGACCGC TCGCTCCGAT TCTCTCCGTC AGAGGTCAAC AGAAACCACC 
ACGGGAACGT GTGCAAGCAC AAGGAGCCGG CGCAGCTCGG GCATGAGTAA GCGAGGGCCA 
GGGGGCTCTG GCCGACAGGA GGGCAGAACT GGAGGAAGGG ATGCTAATAC TGACGCCAAC 
CCAACTAATG CTCCTACCGC ATCTGACGAG TCAGCACAGG GAGCCACTGC CGCAGGATCC 
AGCACAGGCG CCTCGGCCTT AGCAGCCGGC AGCAAAGGCG ACGCTGACGA GTCTGAAGTT 
ACGAGACTAC AAGCTCTTCT GGAGTCCCGA GGGGTACCTC CACATCTGTT CAACGCTCTT 
GGACCGCGCA TGCACCAGCT TCTACAACGC AGCATGGGGT CTGGAGCTAG CAGCAAAGCT 
CACCAGCTAT TATCAGGCCT GCAGGCCAGC GGAGACGAGA GCCAGCAGCT TCAGGCTGTG 
ATCGAGATGT GCCAGCTCCT GGTGATGGGC AACGAGGACA CGCTGGGGGG ATTCCCTGTC 
AAGCAGGTGG TCCCCGCCCT CATCAGCCTA CTCTCCATGG AACATAACTT TGACATCATG 
AACCATGCCT GCCGCGCATT GACCTACATG ATGGAAGCTC TACCGAGGTC ATCGTCTGTT 
GTCATGGATG CAATACCGGT ATTCCTAGAG AAGCTCCAAG TGATCCAGTG CATGGATGTC 
GCCGAGCAAT CTCTAACGGC TCTGGAGATG CTGTCTCGAA GACACAGCAA AGCCATACTT 
CAGGCAGGTG GTCTCTCTGC ATGCCTTCTC TACATCGAGT TCTTCTCAAT AACTGCCCAG 
CGAAATGCCT TGGCTGTTGC AGCTAACTGT TGCCAAAGCA TGGGACCGGA TGAATTTAAT 
TTGGTTGCCA ACTCGTTACC ATTGCTCACT GGAAGACTTC AACATCAGGA TAAGAAGTCT 
GTAGAGAGCT GTTGCCTGTG TTTCGCCCGT CTCGTGGATA ACTATCAGAA TGATGAAAAG 
CTTCTATTGC TCATAGCAGA ACATGGTCTT CTCACCAACC TACAACAACT GCTGGTAGTG 
ACACCCCCTA TCATCAGTAC AGGGACGTTC ATCATGGTGA TTCGTATGCT ATCACTCATG 
TGTGCAAACT GCCCCAGCCT CGCTGTGCAG CTGCTTAAAC AAAATATTGC AGAAACTATC 
AGCTACCTCC TGATTGGAGC TTCAGACTCT GCCAATGTAG ATATTGAGCT TGTATCAAGG 
AGTCCCCAGG AGCTGTATGA AATCACCTCA CTGATTGGTG AGTTGATGCC TAGGCTTCCC 
CGTGATGGAT TGTTCTCCGT CGATGCCTAC CTCAAGAAGG TACCTAACCA GATCACTGAA 
GCAGCCCTGT GGCAGTGGAG GGACGACAGG GGAATGTGGC ATCCGTATAC TCGCATCGAT 
AACAGGATTG TCGAGGCTGC CCATCAAGCA GGAGAAGACG AGGTGACCCT CTCCACCCTG 
GGGCGGACCT ACACCATAGA CTTTAACGCC ATGCAGCAGA TCAATGAGGA CACTGGCACT 
GCTAGACCGG TCCAGAGAAG GCCTAACCCT GCGGCAGGAG CTGGTTCCTC TTCAGCTTCA 
GCAGTAGCAG CTCCCAAGGA AGATGCACGA GTTGTTTTCA TGAAGGAAGA TGGTGACCAG 
TCAGCTGCAT TCATTAAGTC ACTCTTTGCT GTCCTCTACG AAGTCTACAG CTCATCGGCT 
GGACCTGCAG TCAGGCATAA ATGTTTGAAG GCCTTACTAA GAATGGTGTT CTTTGGCAAG 
CCAGATCTCC TGAAGGATAT GCTGAAGAAT CATGCTGTAT CCAGTCACAT AGCAGCCATG 
CTATCGTCTC AAGACCTCAA GGTTGTCGTA GGCGCCCTTC AGATGTCTGA GATCCTCATG 
CAGAAGCTCC CGGATATCTT CCATATCTAC TTCCGCAGAG AAGGTGTGAT GCATCAAGTG 
AAGAACCTCG CTGAGGCCAC CATCACGGCG GACAACTCTC CTCCCATCAA AGATAAGTTC 
CCCATCTCCA AGGTAACGGC TGCTGACCCC TCCTCCTCCT CCTCGACCAC CCCAGCTTCG 
TCTGCATCCT CTGCCTCCTC GGCGTCATCG GCGTCATCTG CATCTTCGGC AGCCTCGTCC 
GCTTCGTCCA CCCATACCCC GTCACCCCGG GATACCATCA ACATCAATGT GGGCTTAGAT 
TCGGATGGTG TACCTCAAGG ACGTCTGAGC GATGCCCTGA AGCTGAAACG AGCAACCAAG 
CGAGGTCCAG GCCGGAAGAG CAACCGCTAC GCTAGCAACG ATGACTCAAC GCCGTCCCAG 
GGAGGTCATG GCCACCACTA CGAGGCCATC GACACCCCAC CGGGCAGACT GAGTGCGGTA 
TCGGTGCCCA TGCCCCCCTC CTCCAGGGCT CCGGGCAGCG GCAGCGGGGG CAGGACAGGG 
GTCAGCAAGG GCAAAGGCTC TACAGGCAAG ATGGTCAGCG GGTCCACCTC CTCACGTTCT 
TCCTTCCTGG CCAGTCTCAA CCTCTCCCGC TGGGGCAGGG GCGCGTCCAA CTCCCCCTCC 
TCCGCCAACA CCACCCCGAG CAACGCCGCC TTAGGTAGCG ACCACCACCA CCACCACCAT 
CACCATCACC ATCACCACCA CCTGCACACC GCGGCAGGGC GCGAGATGGG CCTGCAGAGG 
GGCGGAGCCG GGGGAGGAAT GGGGTACGGG TCGGACAGGA TACTAGGGCA CGGGAGTGAG 
AACCTCCTAG GGGTGCAGAA GGATGCCAGC GGATCGCAGA AGGAAAAAGT CAGGGCCTGG 
ATCAAGGAAC AAGCATCTCA GTTTATACGT CAGTACTTCA GCCAAACAGA TCACCAGGGA 
GAATCACACC CAGCCCTCAC CTTACTCAAT CAGCTCTGTA CCAATGCAGA CAAACTGACA 
CTAAAGAGTG ATGAAGGCTG CGAAATGCTC CGGAGCATTG CTCACATCCT CACCAATTCG 
GACATATCCG CCTTTGAGAT CCTGCACAGC GGTCTGGTGG TCAAACTCTT GGACTACATG 
ACCAGCAACA GTGACCACAA TGCCGTCCCG AGGGATCTTA GACTGCAACG TTTCCTACAT 
ATATTCCTAG AATGCCCAGC TCCAGGCAGT CCTCCTTTCA CCTCACTGGA GATCCCCGAG 
AGCGCGCCCT TCGCTTCCCT CGTCTACAAG ATCAACGGCT GCATCAGCCA CCTGGAGCAG 
TTCCCGGTCA AGGTTCATGA CCTGCCCGGC GCCGGCGGGT CATCGGGAGG CACCAGGGGT 
TCCCAGGCAC TCAAGTTCTT CAACACCCAT CAGCTCAAGT GCCAGCTGCA GAGGCATCCC 
GAGTGCACCA ACCTGAGGCA GTGGAGGGGC GGACCGGTCA AGATTGACCC GCTGGCCCTG 
GTGCAAGCCA TCGAGAGGTA CCTCATGGTC AGGAACTACG GACGCATGAG ACAGGATGAT 
GAGGATAGTG ATGATGATGC ATCGGACGAT GAGATTGATG AGTCTGTGGC AACGGCCTTC 
TCCAGCTCCA ACACCAACAT CAAGCACAAA CTCCAGTTCT TGATCGGAGA GCAGGTCCTA 
CCGTACAACA TGACAGTCTA CCAGGCCATC AAGCAGTTTG GTAACGCTGC TAGTGCATCG 
GAAGACGATC GGGATACAGA TGATGAGAGC AACCCGCTAG GAAGGGCAGG TATTTGGATC 
AAGACACACA CGATATGGTA CCGACCCGTC CCAGAGGCTG GTGCGCCCTC CACCCAGAAG 
TCATCATCGG CATCGAACAG CAGCACGACC ACCACCACGA CCTCCACCAG GAAAGGCAAG 
GGAGGGAGCT CGTCCAAGTC CTCTTCTAAG GGAAGAAAAC ATGATGAGAT GTGGATGGAT 
GGTGTGTGTC CAGTAATACA GAAGGAGCTC CATAAGTACC TACATAGCAA TCTACCTGGT 
TCCGTCCCTA TCGAAGACCC CTCTTGTGCA GCTATCTCAC TGCTAAGGGT TCTCTACGGT 
CTTAGCAGAC ATTGGAACAA TCTTTATCCG GGAGCTTTAC CGCAGCAGGC AGTAAGTAGC 
AGTGAGGTTG TGAATAGCAA GCTGTCGGCC AAGGCTAACA GACAGCTACA GGATCCTTTA 
GTAATAATGA CTGGCAACCT ACCTTCATGG CTCAACGCAG TGGCACAGGC ATGTCCGTTC 
CTGCTGCCGT TTGACACCCG TCAGCTCCTG TTCTACACCA CAGCGTTTGA CAGGGATCGA 
GCCATGCAGA GGCTACAGGA CAACAACCCA GACATGTCCA CATCAGAGAA TAGTGAGAGG 
GTTGCTCCTA GATTGGACAG AAGGAAAAGA AGTGTATCAA GAGCAGATCT GTTACGGCAG 
GCTGAGTCGG TCATGGAGGA TCTAGCTAAC TCCAGGGCAG TGCTGGAAAT ACAGTACGAA 
GATGAGGTTG GTACTGGTTT AGGTCCGACC CTCGAGTTCT ACGCCCTGGT ATCAAGAGAG 
GTACAGCGAG CTGATCTCGA TCTCTGGAGA GGAGAATCGG TTCTTTTACC AGATCCTAAA 
GGCAAGCAAG CTGGAGTGAA GTACATCCAT TCGCCCCACG GTCTCTTCCC ATCCCCGATG 
GGACGGAACG CCAAGGCCTC CGTAGTCGCT AAGATCAAGT CCAAATTTAC CTTCATGGGA 
CGTTTCATCG CAAAGGCACT CATGGACTCT AGAATGATTG ACATTCCACT TAGCCTGCCA 
TTTTTCAAGT GGCTCCTGGG GACGGAGGCT TCGCTGACCT CGTCTGACCT TCAGTTCATT 
GACCGCACCT TGGCCCGATC CTTCCAACAG CTTGAGGAGC TTGGTCAGCA GAAACAACGG 
CTGGTCAATG ACCCCTCTCA GACTGCTGCC AGTCTCCAGC TTGCCCTGGA GTCATTGAGC 
ATAGACGGTA GCCCAGTGGA GGAGCTAGGG CTAGACTTCA CGCTGCCAGG CTATCCTCAT 
ATAGAACTGA AGAAAGGAGG CAAGGACATC GATGTCACCA TTCATAATAT AGATGAGTAC 
CTCAAGCTTG TTGTTCGTTG GACCCTGAAC GAGGGCGTAT CCAGGCAGTT TGAAGCTCTT 
AAAGATGGAT TCCAGTCCGT GTTCCCTCTC TCTCATCTGC AAAGCTTCTA CGGAGAAGAG 
TTGGATCAGC TGTTCTGTGG GAATCGTAGT GAGCCTTGGG ATATCAAGAC GTTACTAGAA 
TGCTGCAGGC CTGATCACGG CTATACTCAT GATAGTCGTG CTGTGAAGCT GTTATTTGAG 
CTCATGGCGT CGTACACAGA CAGTCAACAG AGGGAGTTTC TTCAGTTCGT CACTGGAAGC 
CCCAAACTCC CTGTAGGAGG TTTCCGTTCC CTGAATCCTC CTCTGACGAT CGTACGTAAG 
ATGTTCGAAG GCTCCGAGGA CCCGGACAAA TTCCTTCCCT CTGTGATGAC TTGTGTGAAC 
TACCTGAAGC TCCCTGACTA TACCTCCATG GAAGTCATGG CGTCCAAGCT TTCTATAGCC 
ACGCGAGAAG GACAACAGTC TTTCCATTTA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011676008.1
CDS	112..6927
Translation	MICSCESRYHSPDLRNLWECLVPGPVPIMADQSPSCHQLGGSKHRRSKRGATATTSTTTSNAVASSTAGSSVASLVQDLSNHQKHQVSSRQETCIKSSKKQTLTSASKRKASTESHGAPPSKVSSSTLLDASNADSSSGAQLIKNNRHKHEVCIPVTTLKSKRHNLPKKSKKTVELSKHNLLQSEDSVQNNVSGNESAHSRDSKQRRRGSTSSASSAWSSLSGGSSRFSLSDSTGSVCFAGSSFTSAALPTGELEGSEPSVSSDLKSGRVNINITSRSLRSYSRHHPYQFEALDYYSPRRLGLRTTTTLSTSFTKSLEEPKLNSVTFGEPQTKANDLTASATKAKSLTPSDQAGSKKAKGTVSKTAIQKTPVQAAAQAKRKSNKKLAKPATAKQSSSASASQPKARKQSSNHKATVKGKGKGRSSTARSDSLRQRSTETTTGTCASTRSRRSSGMSKRGPGGSGRQEGRTGGRDANTDANPTNAPTASDESAQGATAAGSSTGASALAAGSKGDADESEVTRLQALLESRGVPPHLFNALGPRMHQLLQRSMGSGASSKAHQLLSGLQASGDESQQLQAVIEMCQLLVMGNEDTLGGFPVKQVVPALISLLSMEHNFDIMNHACRALTYMMEALPRSSSVVMDAIPVFLEKLQVIQCMDVAEQSLTALEMLSRRHSKAILQAGGLSACLLYIEFFSITAQRNALAVAANCCQSMGPDEFNLVANSLPLLTGRLQHQDKKSVESCCLCFARLVDNYQNDEKLLLLIAEHGLLTNLQQLLVVTPPIISTGTFIMVIRMLSLMCANCPSLAVQLLKQNIAETISYLLIGASDSANVDIELVSRSPQELYEITSLIGELMPRLPRDGLFSVDAYLKKVPNQITEAALWQWRDDRGMWHPYTRIDNRIVEAAHQAGEDEVTLSTLGRTYTIDFNAMQQINEDTGTARPVQRRPNPAAGAGSSSASAVAAPKEDARVVFMKEDGDQSAAFIKSLFAVLYEVYSSSAGPAVRHKCLKALLRMVFFGKPDLLKDMLKNHAVSSHIAAMLSSQDLKVVVGALQMSEILMQKLPDIFHIYFRREGVMHQVKNLAEATITADNSPPIKDKFPISKVTAADPSSSSSTTPASSASSASSASSASSASSAASSASSTHTPSPRDTININVGLDSDGVPQGRLSDALKLKRATKRGPGRKSNRYASNDDSTPSQGGHGHHYEAIDTPPGRLSAVSVPMPPSSRAPGSGSGGRTGVSKGKGSTGKMVSGSTSSRSSFLASLNLSRWGRGASNSPSSANTTPSNAALGSDHHHHHHHHHHHHHLHTAAGREMGLQRGGAGGGMGYGSDRILGHGSENLLGVQKDASGSQKEKVRAWIKEQASQFIRQYFSQTDHQGESHPALTLLNQLCTNADKLTLKSDEGCEMLRSIAHILTNSDISAFEILHSGLVVKLLDYMTSNSDHNAVPRDLRLQRFLHIFLECPAPGSPPFTSLEIPESAPFASLVYKINGCISHLEQFPVKVHDLPGAGGSSGGTRGSQALKFFNTHQLKCQLQRHPECTNLRQWRGGPVKIDPLALVQAIERYLMVRNYGRMRQDDEDSDDDASDDEIDESVATAFSSSNTNIKHKLQFLIGEQVLPYNMTVYQAIKQFGNAASASEDDRDTDDESNPLGRAGIWIKTHTIWYRPVPEAGAPSTQKSSSASNSSTTTTTTSTRKGKGGSSSKSSSKGRKHDEMWMDGVCPVIQKELHKYLHSNLPGSVPIEDPSCAAISLLRVLYGLSRHWNNLYPGALPQQAVSSSEVVNSKLSAKANRQLQDPLVIMTGNLPSWLNAVAQACPFLLPFDTRQLLFYTTAFDRDRAMQRLQDNNPDMSTSENSERVAPRLDRRKRSVSRADLLRQAESVMEDLANSRAVLEIQYEDEVGTGLGPTLEFYALVSREVQRADLDLWRGESVLLPDPKGKQAGVKYIHSPHGLFPSPMGRNAKASVVAKIKSKFTFMGRFIAKALMDSRMIDIPLSLPFFKWLLGTEASLTSSDLQFIDRTLARSFQQLEELGQQKQRLVNDPSQTAASLQLALESLSIDGSPVEELGLDFTLPGYPHIELKKGGKDIDVTIHNIDEYLKLVVRWTLNEGVSRQFEALKDGFQSVFPLSHLQSFYGEELDQLFCGNRSEPWDIKTLLECCRPDHGYTHDSRAVKLLFELMASYTDSQQREFLQFVTGSPKLPVGGFRSLNPPLTIVRKMFEGSEDPDKFLPSVMTCVNYLKLPDYTSMEVMASKLSIATREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011677706.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus E3 ubiquitin-protein ligase TRIP12 (LOC578329), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011677706.1

ORF Insert Sequence:

ATGATTTGCT CTTGTGAGAG CAGATATCAT TCGCCTGACC TGAGGAACCT TTGGGAATGT 
CTCGTCCCAG GACCGGTGCC TATTATGGCC GACCAGAGCC CTAGCTGCCA CCAATTAGGG 
GGGTCCAAAC ACCGACGATC AAAGCGAGGT GCCACTGCTA CCACGTCTAC CACTACTAGC 
AACGCCGTGG CCTCCTCGAC CGCTGGATCT TCGGTAGCCA GTCTTGTACA AGACCTATCA 
AATCACCAGA AGCATCAAGT GTCCTCTAGA CAGGAGACTT GCATCAAGTC AAGCAAGAAG 
CAAACTCTCA CCAGTGCAAG CAAGAGGAAG GCTTCAACCG AGAGCCACGG AGCTCCACCT 
TCCAAGGTAT CATCAAGTAC TCTCTTGGAT GCCTCCAACG CTGACAGCAG CAGCGGTGCT 
CAGCTAATCA AAAACAATAG ACACAAACAC GAAGTGTGCA TTCCAGTGAC CACTCTCAAG 
AGCAAGCGTC ACAACCTCCC AAAGAAATCC AAAAAGACTG TCGAGCTTTC CAAGCACAAC 
CTCCTGCAGA GCGAGGACTC TGTCCAGAAC AACGTCAGCG GCAACGAGAG CGCGCACTCG 
CGGGACTCTA AGCAGCGCAG GCGCGGGAGC ACGTCTTCCG CTTCGTCAGC GTGGTCTTCC 
CTCTCTGGAG GATCAAGTCG TTTTAGCTTA TCGGACTCCA CAGGCAGCGT CTGTTTCGCC 
GGGAGTTCTT TCACAAGTGC AGCCCTTCCA ACGGGGGAAC TAGAGGGCTC CGAACCTAGT 
GTGTCGAGTG ATTTGAAAAG TGGCCGGGTG AATATAAACA TTACATCCCG CTCCCTACGC 
TCATACTCCC GACATCACCC CTACCAATTT GAGGCCTTGG ACTACTACTC CCCGCGCCGC 
CTAGGTCTCA GGACTACCAC CACCCTTTCC ACCTCCTTCA CCAAGTCGTT GGAGGAACCC 
AAACTGAACA GTGTTACCTT TGGTGAACCT CAAACCAAAG CAAACGATCT GACCGCAAGT 
GCTACCAAAG CCAAAAGCCT TACGCCAAGC GACCAAGCAG GTTCTAAGAA GGCCAAAGGG 
ACGGTCTCCA AAACTGCAAT ACAGAAAACC CCTGTGCAAG CAGCAGCACA GGCCAAGAGG 
AAAAGCAATA AGAAACTTGC CAAGCCCGCT ACCGCCAAGC AATCTTCATC GGCTTCTGCG 
TCACAGCCAA AAGCCAGAAA GCAGAGTAGC AACCACAAAG CAACTGTCAA AGGGAAGGGA 
AAGGGCCGGT CATCGACCGC TCGCTCCGAT TCTCTCCGTC AGAGGTCAAC AGAAACCACC 
ACGGGAACGT GTGCAAGCAC AAGGAGCCGG CGCAGCTCGG GCATGAGTAA GCGAGGGCCA 
GGGGGCTCTG GCCGACAGGA GGGCAGAACT GGAGGAAGGG ATGCTAATAC TGACGCCAAC 
CCAACTAATG CTCCTACCGC ATCTGACGAG TCAGCACAGG GAGCCACTGC CGCAGGATCC 
AGCACAGGCG CCTCGGCCTT AGCAGCCGGC AGCAAAGGCG ACGCTGACGA GTCTGAAGTT 
ACGAGACTAC AAGCTCTTCT GGAGTCCCGA GGGGTACCTC CACATCTGTT CAACGCTCTT 
GGACCGCGCA TGCACCAGCT TCTACAACGC AGCATGGGGT CTGGAGCTAG CAGCAAAGCT 
CACCAGCTAT TATCAGGCCT GCAGGCCAGC GGAGACGAGA GCCAGCAGCT TCAGGCTGTG 
ATCGAGATGT GCCAGCTCCT GGTGATGGGC AACGAGGACA CGCTGGGGGG ATTCCCTGTC 
AAGCAGGTGG TCCCCGCCCT CATCAGCCTA CTCTCCATGG AACATAACTT TGACATCATG 
AACCATGCCT GCCGCGCATT GACCTACATG ATGGAAGCTC TACCGAGGTC ATCGTCTGTT 
GTCATGGATG CAATACCGGT ATTCCTAGAG AAGCTCCAAG TGATCCAGTG CATGGATGTC 
GCCGAGCAAT CTCTAACGGC TCTGGAGATG CTGTCTCGAA GACACAGCAA AGCCATACTT 
CAGGCAGGTG GTCTCTCTGC ATGCCTTCTC TACATCGAGT TCTTCTCAAT AACTGCCCAG 
CGAAATGCCT TGGCTGTTGC AGCTAACTGT TGCCAAAGCA TGGGACCGGA TGAATTTAAT 
TTGGTTGCCA ACTCGTTACC ATTGCTCACT GGAAGACTTC AACATCAGGA TAAGAAGTCT 
GTAGAGAGCT GTTGCCTGTG TTTCGCCCGT CTCGTGGATA ACTATCAGAA TGATGAAAAG 
CTTCTATTGC TCATAGCAGA ACATGGTCTT CTCACCAACC TACAACAACT GCTGGTAGTG 
ACACCCCCTA TCATCAGTAC AGGGACGTTC ATCATGGTGA TTCGTATGCT ATCACTCATG 
TGTGCAAACT GCCCCAGCCT CGCTGTGCAG CTGCTTAAAC AAAATATTGC AGAAACTATC 
AGCTACCTCC TGATTGGAGC TTCAGACTCT GCCAATGTAG ATATTGAGCT TGTATCAAGG 
AGTCCCCAGG AGCTGTATGA AATCACCTCA CTGATTGGTG AGTTGATGCC TAGGCTTCCC 
CGTGATGGAT TGTTCTCCGT CGATGCCTAC CTCAAGAAGG TACCTAACCA GATCACTGAA 
GCAGCCCTGT GGCAGTGGAG GGACGACAGG GGAATGTGGC ATCCGTATAC TCGCATCGAT 
AACAGGATTG TCGAGGCTGC CCATCAAGCA GGAGAAGACG AGGTGACCCT CTCCACCCTG 
GGGCGGACCT ACACCATAGA CTTTAACGCC ATGCAGCAGA TCAATGAGGA CACTGGCACT 
GCTAGACCGG TCCAGAGAAG GCCTAACCCT GCGGCAGGAG CTGGTTCCTC TTCAGCTTCA 
GCAGTAGCAG CTCCCAAGGA AGATGCACGA GTTGTTTTCA TGAAGGAAGA TGGTGACCAG 
TCAGCTGCAT TCATTAAGTC ACTCTTTGCT GTCCTCTACG AAGTCTACAG CTCATCGGCT 
GGACCTGCAG TCAGGCATAA ATGTTTGAAG GCCTTACTAA GAATGGTGTT CTTTGGCAAG 
CCAGATCTCC TGAAGGATAT GCTGAAGAAT CATGCTGTAT CCAGTCACAT AGCAGCCATG 
CTATCGTCTC AAGACCTCAA GGTTGTCGTA GGCGCCCTTC AGATGTCTGA GATCCTCATG 
CAGAAGCTCC CGGATATCTT CCATATCTAC TTCCGCAGAG AAGGTGTGAT GCATCAAGTG 
AAGAACCTCG CTGAGGCCAC CATCACGGCG GACAACTCTC CTCCCATCAA AGATAAGTTC 
CCCATCTCCA AGGTAACGGC TGCTGACCCC TCCTCCTCCT CCTCGACCAC CCCAGCTTCG 
TCTGCATCCT CTGCCTCCTC GGCGTCATCG GCGTCATCTG CATCTTCGGC AGCCTCGTCC 
GCTTCGTCCA CCCATACCCC GTCACCCCGG GATACCATCA ACATCAATGT GGGCTTAGAT 
TCGGATGGTG TACCTCAAGG ACGTCTGAGC GATGCCCTGA AGCTGAAACG AGCAACCAAG 
CGAGGTCCAG GCCGGAAGAG CAACCGCTAC GCTAGCAACG ATGACTCAAC GCCGTCCCAG 
GGAGGTCATG GCCACCACTA CGAGGCCATC GACACCCCAC CGGGCAGACT GAGTGCGGTA 
TCGGTGCCCA TGCCCCCCTC CTCCAGGGCT CCGGGCAGCG GCAGCGGGGG CAGGACAGGG 
GTCAGCAAGG GCAAAGGCTC TACAGGCAAG ATGGTCAGCG GGTCCACCTC CTCACGTTCT 
TCCTTCCTGG CCAGTCTCAA CCTCTCCCGC TGGGGCAGGG GCGCGTCCAA CTCCCCCTCC 
TCCGCCAACA CCACCCCGAG CAACGCCGCC TTAGGTAGCG ACCACCACCA CCACCACCAT 
CACCATCACC ATCACCACCA CCTGCACACC GCGGCAGGGC GCGAGATGGG CCTGCAGAGG 
GGCGGAGCCG GGGGAGGAAT GGGGTACGGG TCGGACAGGA TACTAGGGCA CGGGAGTGAG 
AACCTCCTAG GGGTGCAGAA GGATGCCAGC GGATCGCAGA AGGAAAAAGT CAGGGCCTGG 
ATCAAGGAAC AAGCATCTCA GTTTATACGT CAGTACTTCA GCCAAACAGA TCACCAGGGA 
GAATCACACC CAGCCCTCAC CTTACTCAAT CAGCTCTGTA CCAATGCAGA CAAACTGACA 
CTAAAGAGTG ATGAAGGCTG CGAAATGCTC CGGAGCATTG CTCACATCCT CACCAATTCG 
GACATATCCG CCTTTGAGAT CCTGCACAGC GGTCTGGTGG TCAAACTCTT GGACTACATG 
ACCAGCAACA GTGACCACAA TGCCGTCCCG AGGGATCTTA GACTGCAACG TTTCCTACAT 
ATATTCCTAG AATGCCCAGC TCCAGGCAGT CCTCCTTTCA CCTCACTGGA GATCCCCGAG 
AGCGCGCCCT TCGCTTCCCT CGTCTACAAG ATCAACGGCT GCATCAGCCA CCTGGAGCAG 
TTCCCGGTCA AGGTTCATGA CCTGCCCGGC GCCGGCGGGT CATCGGGAGG CACCAGGGGT 
TCCCAGGCAC TCAAGTTCTT CAACACCCAT CAGCTCAAGT GCCAGCTGCA GAGGCATCCC 
GAGTGCACCA ACCTGAGGCA GTGGAGGGGC GGACCGGTCA AGATTGACCC GCTGGCCCTG 
GTGCAAGCCA TCGAGAGGTA CCTCATGGTC AGGAACTACG GACGCATGAG ACAGGATGAT 
GAGGATAGTG ATGATGATGC ATCGGACGAT GAGATTGATG AGTCTGTGGC AACGGCCTTC 
TCCAGCTCCA ACACCAACAT CAAGCACAAA CTCCAGTTCT TGATCGGAGA GCAGGTCCTA 
CCGTACAACA TGACAGTCTA CCAGGCCATC AAGCAGTTTG GTAACGCTGC TAGTGCATCG 
GAAGACGATC GGGATACAGA TGATGAGAGC AACCCGCTAG GAAGGGCAGG TATTTGGATC 
AAGACACACA CGATATGGTA CCGACCCGTC CCAGAGGCTG GTGCGCCCTC CACCCAGAAG 
TCATCATCGG CATCGAACAG CAGCACGACC ACCACCACGA CCTCCACCAG GAAAGGCAAG 
GGAGGGAGCT CGTCCAAGTC CTCTTCTAAG GGAAGAAAAC ATGATGAGAT GTGGATGGAT 
GGTGTGTGTC CAGTAATACA GAAGGAGCTC CATAAGTACC TACATAGCAA TCTACCTGGT 
TCCGTCCCTA TCGAAGACCC CTCTTGTGCA GCTATCTCAC TGCTAAGGGT TCTCTACGGT 
CTTAGCAGAC ATTGGAACAA TCTTTATCCG GGAGCTTTAC CGCAGCAGGC AGTAAGTAGC 
AGTGAGGTTG TGAATAGCAA GCTGTCGGCC AAGGCTAACA GACAGCTACA GGATCCTTTA 
GTAATAATGA CTGGCAACCT ACCTTCATGG CTCAACGCAG TGGCACAGGC ATGTCCGTTC 
CTGCTGCCGT TTGACACCCG TCAGCTCCTG TTCTACACCA CAGCGTTTGA CAGGGATCGA 
GCCATGCAGA GGCTACAGGA CAACAACCCA GACATGTCCA CATCAGAGAA TAGTGAGAGG 
GTTGCTCCTA GATTGGACAG AAGGAAAAGA AGTGTATCAA GAGCAGATCT GTTACGGCAG 
GCTGAGTCGG TCATGGAGGA TCTAGCTAAC TCCAGGGCAG TGCTGGAAAT ACAGTACGAA 
GATGAGGTTG GTACTGGTTT AGGTCCGACC CTCGAGTTCT ACGCCCTGGT ATCAAGAGAG 
GTACAGCGAG CTGATCTCGA TCTCTGGAGA GGAGAATCGG TTCTTTTACC AGATCCTAAA 
GGCAAGCAAG CTGGAGTGAA GTACATCCAT TCGCCCCACG GTCTCTTCCC ATCCCCGATG 
GGACGGAACG CCAAGGCCTC CGTAGTCGCT AAGATCAAGT CCAAATTTAC CTTCATGGGA 
CGTTTCATCG CAAAGGCACT CATGGACTCT AGAATGATTG ACATTCCACT TAGCCTGCCA 
TTTTTCAAGT GGCTCCTGGG GACGGAGGCT TCGCTGACCT CGTCTGACCT TCAGTTCATT 
GACCGCACCT TGGCCCGATC CTTCCAACAG CTTGAGGAGC TTGGTCAGCA GAAACAACGG 
CTGGTCAATG ACCCCTCTCA GACTGCTGCC AGTCTCCAGC TTGCCCTGGA GTCATTGAGC 
ATAGACGGTA GCCCAGTGGA GGAGCTAGGG CTAGACTTCA CGCTGCCAGG CTATCCTCAT 
ATAGAACTGA AGAAAGGAGG CAAGGACATC GATGTCACCA TTCATAATAT AGATGAGTAC 
CTCAAGCTTG TTGTTCGTTG GACCCTGAAC GAGGGCGTAT CCAGGCAGTT TGAAGCTCTT 
AAAGATGGAT TCCAGTCCGT GTTCCCTCTC TCTCATCTGC AAAGCTTCTA CGGAGAAGAG 
TTGGATCAGC TGTTCTGTGG GAATCGTAGT GAGCCTTGGG ATATCAAGAC GTTACTAGAA 
TGCTGCAGGC CTGATCACGG CTATACTCAT GATAGTCGTG CTGTGAAGCT GTTATTTGAG 
CTCATGGCGT CGTACACAGA CAGTCAACAG AGGGAGTTTC TTCAGTTCGT CACTGGAAGC 
CCCAAACTCC CTGTAGGAGG TTTCCGTTCC CTGAATCCTC CTCTGACGAT CGTACGTAAG 
ATGTTCGAAG GCTCCGAGGA CCCGGACAAA TTCCTTCCCT CTGTGATGAC TTGTGTGAAC 
TACCTGAAGC TCCCTGACTA TACCTCCATG GAAGTCATGG CGTCCAAGCT TTCTATAGCC 
ACGCGAGAAG GACAACAGTC TTTCCATTTA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC578329 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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