bag6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	bag6
Entrez Gene ID	100038304
Full Name	BCL2 associated athanogene 6
Synonyms	Bat-3,Scythe,bag-6,bag6-a,bag6-b,bat3,d6s52e
General protein information	Preferred Names BCL2 associated athanogene 6 Names large proline-rich protein bag6 HLA-B-associated transcript 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012967839.3	XP_012823293.2	large proline-rich protein bag6 isoform X3
XM_018096092.1	XP_017951581.1	large proline-rich protein bag6 isoform X2
NM_001097409.2	NP_001090878.2	large proline-rich protein bag6 isoform 1
XM_012967838.3	XP_012823292.2	large proline-rich protein bag6 isoform X1
XM_012967838.2	XP_012823292.1	large proline-rich protein bag6 isoform X1
XM_012967839.2	XP_012823293.1	large proline-rich protein bag6 isoform X3
NM_001244955.1	NP_001231884.1	large proline-rich protein bag6 isoform 2
XM_018096092.2	XP_017951581.2	large proline-rich protein bag6 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	BAG6	NP_004630.3
Canis lupus familiaris (dog)	BAG6	XP_005627197.1
Bos taurus (cattle)	BAG6	NP_001068834.2
Xenopus tropicalis (tropical clawed frog)	bag6	NP_001231884.1
Mus musculus (house mouse)	Bag6	NP_001239398.1
Rattus norvegicus (Norway rat)	Bag6	NP_001029140.1
Danio rerio (zebrafish)	bag6	NP_958445.1

Gene Ontology
Bibliography

Related articles in PubMed

A conserved island of BAG6/Scythe is related to ubiquitin domains and participates in short hydrophobicity recognition.
Tanaka H, Takahashi T, Xie Y, Minami R, Yanagi Y, Hayashishita M, Suzuki R, Yokota N, Shimada M, Mizushima T, Kuwabara N, Kato R, Kawahara H
The FEBS journal283(4)662-77(2016 Feb)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The observations suggest a mechanism whereby the BAG6 ubiquitin-linked domain provides a platform for discriminating substrates with shorter hydrophobicity stretches as a signal for defective proteins.
Title: A conserved island of BAG6/Scythe is related to ubiquitin domains and participates in short hydrophobicity recognition.

The following bag6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bag6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45928	XM_018096092.1 Latest version!	Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa63367	XM_012967838.3 Latest version!	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa63368	XM_018096092.2 Latest version!	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa63369	XM_012967839.3 Latest version!	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa02170	NM_001097409.2 Latest version!	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa02171	NM_001244955.1 Latest version!	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa30198	XM_012967838.2 Latest version!	Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa30199	XM_012967839.2 Latest version!	Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bag6 ( XM_018096092.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951581.1 Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa45928
Clone ID Related Accession (Same CDS sequence)	XM_018096092.1
Accession Version	XM_018096092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	large proline-rich protein bag6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAAATGATGG CTCCACTCTT GATGTCCATA TAAATCTGGA TCAACAGCTC 
CCTGTCCAGA GTGAACCTCG TGTTCGTCTT GTTTTGGCTC AGCAAATACT GCAGGACATA 
CAGAGGATAC TGGATCGTTT GGAGGGACAA CCAGTTAATG AGCAGACTGC AGAACCGATG 
GACACAGCAG TGTCTGAAGG AGAGGCCAGC AGTAGAGAAA CCTTGCCTCA GACTACACAG 
AATACAGATG GGCAGTCGAA TACAGCCCCT ACCAGTCATC CTTCACCATC AGAATATGTT 
GAGGTCCTGC AGTCTTTGTC ACGGGTTGAG GAACGTCTAG CCCCTTTCAT GCAACGTTAC 
CGGGAAATCC TTAGTAGTGC TACCTCTGAT GCCTATGAGA ATCAGGAAGA AAGGGAGCAG 
TCCCAGCGGA TAATTAATTT AGTTGGAGAA TCTCTTCGTC TCCTGGGAAA TGCCCTGGTT 
GCTGTATCCG ACTTGCGCTG CAATCTCTCT TCTGCTTCTC CCAGACACTT GCATGTTGTT 
CGCCCAATGT CCCATTACAC TGGGCCCATG CTACTACAGC AAGCTGCTAT ACCAATACAG 
ATTAATGTTG GTACTACAGT CACTATGACT GGGAATGGAA CCCATGCTGG TCAGATGCCT 
TCAGATGGGA ATGCTGCTCA TACTCCTACC AATACTTCAG AACCTCAGCG ATCTAACTCT 
GACAACCAAC CTCCATCCAG TGGAGAGAGA CCAGCTTCTG AGATCCCTCC CACTTCAGTT 
CCACATCCCC ACCCTCGTGT TGTACGGATT ACGCACCAGA CTGTTGAGCC TGTCATGATG 
ATGCATATGA ACATTCAGGA TTCTGGACCT GGTGGCCCTA CAAACGTTCC CCCACCAACT 
GCGGGGCATG GGGGAAGTGC ACACATTCAT ATGCCAGGAT TACCCCCTGA GTTCATGCAG 
GCAATTTCTC ACCAGATTAC ACAGCAAGCT GTGGCTGCAG CTTCAGGGCA GCAAATTCCT 
GGCTTCCAAG CTCCACCACG CTTTGTTTTT ACTAGGCCTG CTGCCCCTTC ATTTCCACCC 
CAGCCTGGGG TAGCAACTAC CCCACCAGGA CCTGGAGGGG CAACTACTGC TGTACCTGGG 
GCAACGGTGG GTCCAGCAGG AAATGCCTCC TTGGCTCAGA TGATCAGCGG TCTTGTTGGA 
CAGCTTCTGA TGCACCCAGT TATTGTTGCT CAGGGTGGCA GCAACACCCC CTCTTCTACG 
TCAACCCCAA CTTCTACATC CTCCTCCAGC TCCTCTTCAT CCTCCACTGT CACTACCTCT 
ACAACAACCA CCTCTTCCAC ATCCTTCCCC ACTGTTTCAT CAGGTCCCTC TCCCCAGCCC 
CCACCTGGCA CTGATCAACA CCTCTCTCAG CTCCTGGGCT CTTTACTGGG AACTGCCGGC 
TCAGGGATGT CAAACTTCGC TATGGGTTCG CCGAGTATTA CTGTTACCGT GCCTGGAATG 
CCGGCCTTTT TACAGGGTGT CACAGATATA TTACAGGCTA CACAGACTGT TCCTGTTTCT 
ACTTCTCCTC CCCAGTCTGC TTCTCAGGCT CCTCCTCCTT CATCACCCTC ACCACCTCCT 
GCCCATTCCT CTCCCCCTCC AGCTGCCGCA CCAGAGTCTC TTCCTCCAGA GTTTTTCACT 
AGTGTTGTTC AGGGTGTCCT TTCATCCATG CTGGGGTCTT TGAGTGCAGC TGATCAGAGT 
GGAACAGAAA GCATTGCAGC TTTCATTCAG AGGCTCAGTG GCTCCCACAA CATTTTCCAG 
CCTGATGCAG AGGGACCTGG TGGATTTTTT GGTGACTTAC TCACCTTGAT TTGTCACAAT 
TTCTCCCTGG TCGATATGGT AATGTTACTG CATGGTCATT CCCAGCCTTT GCAGAATCTG 
CAGCCTCAAC TGAGATCCTT CTTTCTTCAG GAGTATCTAC ACCAGGCAGA TCCCACACCT 
AATAACATCC AGATGGCATC AAGAAATCTT ACTAATGGCC TGGAAGAGTA CATAAGAGAA 
AGCTTTGCCT CCGTTACTGT GCGTGATGAT GTTGATATCA CAAGAACAAA CCTTGAATTT 
CTACAGGATC AGTTCAACAG GATCACAACA CACATCCTAC ATTGTGCAGA TTCCACATTC 
GGACAGCGGC TTCTTGAGAT GTGTAACCAG TCTCTCTTTG AATGGCTTGC TCTCAACCTG 
TATTGCCTCC GAGGGGACCA GAGTGCTCTC ACATCTGTCA TTAATGAGAG AATTCGAAGA 
CTCTCTCTAG ATGTTTCACC TGTGCTTGTC TCGTGGGTGA CGTCTGTCCT GTCCCTCCGT 
CTTCAAGTGC TGTTGGGGCA GATGCCTGTG ACTGAGGGCG AGATACAGAG ACATATACGG 
AGAGTGGGAG ATGTTCCGCA GGCTCCTGAA GCATCATCTC AGGATCAACC TATGGAAACC 
ACACCTGTTG ATTGTCAGCA GAATGGTGCT GCCTCTCCAG TTCCTGCCAC CACAGTGGAG 
GAGGTGCTTT TCCTCCCCCC TCAAAGTTCA GTACCCACCA TATGCACAGA TTCTGAGCAC 
CCAACCCAAG AAGATACCGG ATCTGAGCAA TGGGCAGCAT CTGTGCCCCC AGAATGGGTT 
CCTGTAATCA GACAAGATAT GCAGAACCAG CGGAAGATGA AACAGCAGCC ACCCTTGAGT 
GATGCCTACC TGAGTGGCAT GCCTGCAAAG CGCCGCAAAA CAATGCAAGG TGAAGGGCCA 
CACTTGTCCC TCTCTGAAGC TGTGAGCCGT GCAATGAAAG CCACAGGAGC CAAACCAGAG 
AGCAGTACAG ACTGTGTTAG GAGGGAGCTG GATAACAGTG AAGCACAAGG AAGGTACAGA 
GAACAGTTGT GTCAGGATAT CCAGAACATC CTCCAAGACA ATGAGTCCTA CAGTGCCCAG 
AGATTCCCCA ACACACAAAG GGCATTCAGA GGGGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951581.1
CDS	191..3592
Translation	MAANEKMEVTVKTLDSQTRTFTVDAEITVKEFKTHISSDVGISPEKQRLIYQGRVLQEDKKLKEYNVDGKVIHLVERAPPQTQPPTGGPSTSSSTSPSSSNAANVPGAGAPERNGNSYVMVGTFNLPHVMSGLGEASRGPRVSTNDGSTLDVHINLDQQLPVQSEPRVRLVLAQQILQDIQRILDRLEGQPVNEQTAEPMDTAVSEGEASSRETLPQTTQNTDGQSNTAPTSHPSPSEYVEVLQSLSRVEERLAPFMQRYREILSSATSDAYENQEEREQSQRIINLVGESLRLLGNALVAVSDLRCNLSSASPRHLHVVRPMSHYTGPMLLQQAAIPIQINVGTTVTMTGNGTHAGQMPSDGNAAHTPTNTSEPQRSNSDNQPPSSGERPASEIPPTSVPHPHPRVVRITHQTVEPVMMMHMNIQDSGPGGPTNVPPPTAGHGGSAHIHMPGLPPEFMQAISHQITQQAVAAASGQQIPGFQAPPRFVFTRPAAPSFPPQPGVATTPPGPGGATTAVPGATVGPAGNASLAQMISGLVGQLLMHPVIVAQGGSNTPSSTSTPTSTSSSSSSSSSTVTTSTTTTSSTSFPTVSSGPSPQPPPGTDQHLSQLLGSLLGTAGSGMSNFAMGSPSITVTVPGMPAFLQGVTDILQATQTVPVSTSPPQSASQAPPPSSPSPPPAHSSPPPAAAPESLPPEFFTSVVQGVLSSMLGSLSAADQSGTESIAAFIQRLSGSHNIFQPDAEGPGGFFGDLLTLICHNFSLVDMVMLLHGHSQPLQNLQPQLRSFFLQEYLHQADPTPNNIQMASRNLTNGLEEYIRESFASVTVRDDVDITRTNLEFLQDQFNRITTHILHCADSTFGQRLLEMCNQSLFEWLALNLYCLRGDQSALTSVINERIRRLSLDVSPVLVSWVTSVLSLRLQVLLGQMPVTEGEIQRHIRRVGDVPQAPEASSQDQPMETTPVDCQQNGAASPVPATTVEEVLFLPPQSSVPTICTDSEHPTQEDTGSEQWAASVPPEWVPVIRQDMQNQRKMKQQPPLSDAYLSGMPAKRRKTMQGEGPHLSLSEAVSRAMKATGAKPESSTDCVRRELDNSEAQGRYREQLCQDIQNILQDNESYSAQRFPNTQRAFRGDP

Target ORF information:

RefSeq Version	XM_018096092.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096092.1

ORF Insert Sequence:

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAAATGATGG CTCCACTCTT GATGTCCATA TAAATCTGGA TCAACAGCTC 
CCTGTCCAGA GTGAACCTCG TGTTCGTCTT GTTTTGGCTC AGCAAATACT GCAGGACATA 
CAGAGGATAC TGGATCGTTT GGAGGGACAA CCAGTTAATG AGCAGACTGC AGAACCGATG 
GACACAGCAG TGTCTGAAGG AGAGGCCAGC AGTAGAGAAA CCTTGCCTCA GACTACACAG 
AATACAGATG GGCAGTCGAA TACAGCCCCT ACCAGTCATC CTTCACCATC AGAATATGTT 
GAGGTCCTGC AGTCTTTGTC ACGGGTTGAG GAACGTCTAG CCCCTTTCAT GCAACGTTAC 
CGGGAAATCC TTAGTAGTGC TACCTCTGAT GCCTATGAGA ATCAGGAAGA AAGGGAGCAG 
TCCCAGCGGA TAATTAATTT AGTTGGAGAA TCTCTTCGTC TCCTGGGAAA TGCCCTGGTT 
GCTGTATCCG ACTTGCGCTG CAATCTCTCT TCTGCTTCTC CCAGACACTT GCATGTTGTT 
CGCCCAATGT CCCATTACAC TGGGCCCATG CTACTACAGC AAGCTGCTAT ACCAATACAG 
ATTAATGTTG GTACTACAGT CACTATGACT GGGAATGGAA CCCATGCTGG TCAGATGCCT 
TCAGATGGGA ATGCTGCTCA TACTCCTACC AATACTTCAG AACCTCAGCG ATCTAACTCT 
GACAACCAAC CTCCATCCAG TGGAGAGAGA CCAGCTTCTG AGATCCCTCC CACTTCAGTT 
CCACATCCCC ACCCTCGTGT TGTACGGATT ACGCACCAGA CTGTTGAGCC TGTCATGATG 
ATGCATATGA ACATTCAGGA TTCTGGACCT GGTGGCCCTA CAAACGTTCC CCCACCAACT 
GCGGGGCATG GGGGAAGTGC ACACATTCAT ATGCCAGGAT TACCCCCTGA GTTCATGCAG 
GCAATTTCTC ACCAGATTAC ACAGCAAGCT GTGGCTGCAG CTTCAGGGCA GCAAATTCCT 
GGCTTCCAAG CTCCACCACG CTTTGTTTTT ACTAGGCCTG CTGCCCCTTC ATTTCCACCC 
CAGCCTGGGG TAGCAACTAC CCCACCAGGA CCTGGAGGGG CAACTACTGC TGTACCTGGG 
GCAACGGTGG GTCCAGCAGG AAATGCCTCC TTGGCTCAGA TGATCAGCGG TCTTGTTGGA 
CAGCTTCTGA TGCACCCAGT TATTGTTGCT CAGGGTGGCA GCAACACCCC CTCTTCTACG 
TCAACCCCAA CTTCTACATC CTCCTCCAGC TCCTCTTCAT CCTCCACTGT CACTACCTCT 
ACAACAACCA CCTCTTCCAC ATCCTTCCCC ACTGTTTCAT CAGGTCCCTC TCCCCAGCCC 
CCACCTGGCA CTGATCAACA CCTCTCTCAG CTCCTGGGCT CTTTACTGGG AACTGCCGGC 
TCAGGGATGT CAAACTTCGC TATGGGTTCG CCGAGTATTA CTGTTACCGT GCCTGGAATG 
CCGGCCTTTT TACAGGGTGT CACAGATATA TTACAGGCTA CACAGACTGT TCCTGTTTCT 
ACTTCTCCTC CCCAGTCTGC TTCTCAGGCT CCTCCTCCTT CATCACCCTC ACCACCTCCT 
GCCCATTCCT CTCCCCCTCC AGCTGCCGCA CCAGAGTCTC TTCCTCCAGA GTTTTTCACT 
AGTGTTGTTC AGGGTGTCCT TTCATCCATG CTGGGGTCTT TGAGTGCAGC TGATCAGAGT 
GGAACAGAAA GCATTGCAGC TTTCATTCAG AGGCTCAGTG GCTCCCACAA CATTTTCCAG 
CCTGATGCAG AGGGACCTGG TGGATTTTTT GGTGACTTAC TCACCTTGAT TTGTCACAAT 
TTCTCCCTGG TCGATATGGT AATGTTACTG CATGGTCATT CCCAGCCTTT GCAGAATCTG 
CAGCCTCAAC TGAGATCCTT CTTTCTTCAG GAGTATCTAC ACCAGGCAGA TCCCACACCT 
AATAACATCC AGATGGCATC AAGAAATCTT ACTAATGGCC TGGAAGAGTA CATAAGAGAA 
AGCTTTGCCT CCGTTACTGT GCGTGATGAT GTTGATATCA CAAGAACAAA CCTTGAATTT 
CTACAGGATC AGTTCAACAG GATCACAACA CACATCCTAC ATTGTGCAGA TTCCACATTC 
GGACAGCGGC TTCTTGAGAT GTGTAACCAG TCTCTCTTTG AATGGCTTGC TCTCAACCTG 
TATTGCCTCC GAGGGGACCA GAGTGCTCTC ACATCTGTCA TTAATGAGAG AATTCGAAGA 
CTCTCTCTAG ATGTTTCACC TGTGCTTGTC TCGTGGGTGA CGTCTGTCCT GTCCCTCCGT 
CTTCAAGTGC TGTTGGGGCA GATGCCTGTG ACTGAGGGCG AGATACAGAG ACATATACGG 
AGAGTGGGAG ATGTTCCGCA GGCTCCTGAA GCATCATCTC AGGATCAACC TATGGAAACC 
ACACCTGTTG ATTGTCAGCA GAATGGTGCT GCCTCTCCAG TTCCTGCCAC CACAGTGGAG 
GAGGTGCTTT TCCTCCCCCC TCAAAGTTCA GTACCCACCA TATGCACAGA TTCTGAGCAC 
CCAACCCAAG AAGATACCGG ATCTGAGCAA TGGGCAGCAT CTGTGCCCCC AGAATGGGTT 
CCTGTAATCA GACAAGATAT GCAGAACCAG CGGAAGATGA AACAGCAGCC ACCCTTGAGT 
GATGCCTACC TGAGTGGCAT GCCTGCAAAG CGCCGCAAAA CAATGCAAGG TGAAGGGCCA 
CACTTGTCCC TCTCTGAAGC TGTGAGCCGT GCAATGAAAG CCACAGGAGC CAAACCAGAG 
AGCAGTACAG ACTGTGTTAG GAGGGAGCTG GATAACAGTG AAGCACAAGG AAGGTACAGA 
GAACAGTTGT GTCAGGATAT CCAGAACATC CTCCAAGACA ATGAGTCCTA CAGTGCCCAG 
AGATTCCCCA ACACACAAAG GGCATTCAGA GGGGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bag6 ( XM_012967838.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823292.2 Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63367
Clone ID Related Accession (Same CDS sequence)	XM_012967838.3
Accession Version	XM_012967838.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	large proline-rich protein bag6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967838.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT CACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTTCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAGTAAGTGG GAATGATGGC TCCACTCTTG ATGTCCATAT AAATCTGGAT 
CAACAGCTCC CTGTCCAGAG TGAACCTCGT GTTCGTCTTG TTTTGGCTCA GCAAATACTG 
CAGGACATAC AGAGGATACT GGATCGTTTG GAGGGACAAC CAGTTAATGA GCAGACTGCA 
GAACCGATGG ACACAGCAGT GTCTGAAGGA GAGGCCAGCA GTAGAGAAAC CTTGCCTCAG 
ACTACACAGA ATACAGATGG GCAGTCGAAT ACAGCCCCTA CCAGTCATCC TTCACCATCA 
GAATATGTTG AGGTCCTGCA GTCTTTGTCA CGGGTTGAGG AACGTCTAGC CCCTTTCATG 
CAACGTTACC GGGAAATCCT TAGTAGTGCT ACCTCTGATG CCTATGAGAA TCAGGAAGAA 
AGGGAGCAGT CCCAGCGGAT AATTAATTTA GTTGGAGAAT CTCTTCGTCT CCTGGGAAAT 
GCCCTGGTTG CTGTATCCGA CTTGCGCTGC AATCTCTCTT CTGCTTCTCC TAGACACTTG 
CATGTTGTTC GCCCTATGTC CCATTACACT GGGCCCATGC TACTACAGCA AGCTGCTATA 
CCAATACAGA TTAATGTTGG TACTACAGTC ACTATGACTG GGAATGGAAC CCATGCTGGT 
CAGATGCCTT CAGATGGGAA TGCTGCTCAT ACTCCTACCA ATACTTCAGA ACCTCAGCGA 
TCTAACTCTG ACAACCAACC TCCATCCAGT GGAGAGAGAC CAGCTTCTGA GATCCCTCCC 
ACTTCAGTTC CACATCCCCA CCCTCGTGTT GTACGGATTA CGCACCAGAC TGTTGAGCCT 
GTCATGATGA TGCATATGAA CATTCAGGAT TCTGGACCTG GTGGCCCTAC AAACATTCCC 
CCACCAACTG CGGGGCATGG GGGAAGTGCA CACATTCATA TGCCAGGATT ACCCCCTGAG 
TTCATGCAGG CAATTTCTCA CCAGATTACA CAGCAAGCTG TGGCTGCAGC TTCAGGGCAG 
CAAATTCCTG GCTTCCAAGC TCCACCACGC TTTGTTTTTA CTAGGCCTGC TGCCCCTTCA 
TTTCCACCCC AGCCTGGGGT AGCAACTACC CCACCAGGAC CTGGAGGGGC AACTACTGCT 
GTACCTGGGG AAACGGTGGG TCCAGCAGGA AATGCCTCCT TGGCTCAGAT GATCAGCGGT 
CTTGTTGGAC AGCTTCTGAT GCACCCAGTT ATTGTTGCTC AGGGTGGCAG CAACACCCCC 
TCTTCTACGT CAACCCCAAC TTCTACATCC TCCTCCAGCT CCTCTTCATC CTCCACTGTC 
ACTACCTCTA CAACAACCAC CTCTTCCACA TCCTTCCCCA CTGTTTCATC AGGTCCCTCT 
CCCCAGCCCC CACCTGGCAC TGATCAACAC CTCTCTCAGC TCCTGGGCTC TTTACTGGGA 
ACTGCCGGCT CAGGGATGTC AAACTTCGCT ATGGGTTCGC CGAGTATTAC TGTTACCGTG 
CCTGGAATGC CGGCCTTTTT ACAGGGTGTC ACAGATATAT TACAGGCTAC ACAGACTGTT 
CCTGTTTCTA CTTCTCCTCC CCAGTCTGCT TCTCAGGCTC CTCCTCCTTC ATCACCCTCA 
CCACCTCCTG CCCATTCCTC TCCCCCTCCA GCTGCCGCAC CAGAGTCTCT TCCTCCAGAG 
TTTTTCACTA GTGTTGTTCA GGGTGTCCTT TCATCCATGC TGGGGTCTTT GAGTGCAGCT 
GATCAGAGTG GAACAGAAAG CATTGCAGCT TTCATTCAGA GGCTCAGTGG CTCCCACAAC 
ATTTTCCAGC CTGATGCAGA GGGACCTGGT GGATTTTTTG GTGACTTACT CACCTTGATT 
TGTCACAATT TCTCCCTGGT CGATATGGTA ATGTTACTGC ATGGTCATTC CCAGCCTTTG 
CAGAATCTGC AGCCTCAACT GAGATCCTTC TTTCTTCAGG AGTATCTACA CCAGGCAGAT 
CCCACACCTA ATAACATCCA GATGGCATCA AGAAATCTTA CTAATGGCCT GGAAGAGTAC 
ATAAGAGAAA GCTTTGCCTC CGTTACTGTG CGTGATGATG TTGATATCAC AAGAACAAAC 
CTTGAATTTC TACAGGATCA GTTCAACAGG ATCACAACAC ACATCCTACA TTGTGCAGAT 
TCCACATTCG GACAGCGGCT TCTTGAGATG TGTAACCAGT CTCTCTTTGA ATGGCTTGCT 
CTCAACCTGT ATTGCCTCCG AGGGGACCAG AGTGCTCTCA CATCTGTCAT TAATGAGAGA 
ATTCGAAGAC TCTCTCTAGA TGTTTCACCT GTGCTTGTCT CGTGGGTGAC GTCTGTCCTG 
TCCCTCCGTC TTCAAGTGCT CTTGGGGCAG ATGCCTGTGA CTGAGGGCGA GATACAGAGA 
CATATACGGA GAGTGGGAGA TGTTCCGCAG GCTCCTGAAG CATCATCTCA GGATCAACCT 
ATGGAAACCA CACCTGTTGA TTGTCAGCAG AATGGTGCTG CCTCTCCAGT TCCTGCCACC 
ACAGTGGAGG AGGTGCTTTT CCTCCCCCCT CAAAGTTCAG TACCCACCAT ATGCACAGAT 
TCTGAGCACC CAACCCAAGA AGATACCGGA TCTGAGCAAT GGGCAGCATC TGTGCCCCCA 
GAATGGGTTC CTGTAATCAG ACAAGATATG CAGAACCAGC GGAAGATGAA ACAGCAGCCA 
CCCTTGAGTG ATGCCTACCT GAGTGGCATG CCTGCAAAGC GCCGCAAAAC AATGCAAGGT 
GAAGGGCCAC ACTTGTCCCT CTCTGAAGCT GTGAGCCGTG CAATGAAAGC CACAGGAGCC 
AAACCAGAGA GCAGTACAGA CTGTGTTAGG AGGGAGCTGG ATAACAGTGA AGCACAAGGA 
AGGTACAGAG AACAGTTGTG TCAGGATATC CAGAACATCC TCCAAGACAA TGAGTCCTAC 
AGTGCCCAGA GATTCCCCAA CACACAAAGG GCATTCAGAG GGGACCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823292.2
CDS	191..3601
Translation	MAANEKMEVTVKTLDSQTRTFTVDAEITVKEFKTHISSDVGISPEKQRLIYQGRVLQEDKKLKEYNVDGKVIHLVERAPPQTQPSTGGPSTSSSTSPSSSNAANVPGAGAPERNGNSYVMVGTFNLPHVMSGLGEASRGPRVSTVSGNDGSTLDVHINLDQQLPVQSEPRVRLVLAQQILQDIQRILDRLEGQPVNEQTAEPMDTAVSEGEASSRETLPQTTQNTDGQSNTAPTSHPSPSEYVEVLQSLSRVEERLAPFMQRYREILSSATSDAYENQEEREQSQRIINLVGESLRLLGNALVAVSDLRCNLSSASPRHLHVVRPMSHYTGPMLLQQAAIPIQINVGTTVTMTGNGTHAGQMPSDGNAAHTPTNTSEPQRSNSDNQPPSSGERPASEIPPTSVPHPHPRVVRITHQTVEPVMMMHMNIQDSGPGGPTNIPPPTAGHGGSAHIHMPGLPPEFMQAISHQITQQAVAAASGQQIPGFQAPPRFVFTRPAAPSFPPQPGVATTPPGPGGATTAVPGETVGPAGNASLAQMISGLVGQLLMHPVIVAQGGSNTPSSTSTPTSTSSSSSSSSSTVTTSTTTTSSTSFPTVSSGPSPQPPPGTDQHLSQLLGSLLGTAGSGMSNFAMGSPSITVTVPGMPAFLQGVTDILQATQTVPVSTSPPQSASQAPPPSSPSPPPAHSSPPPAAAPESLPPEFFTSVVQGVLSSMLGSLSAADQSGTESIAAFIQRLSGSHNIFQPDAEGPGGFFGDLLTLICHNFSLVDMVMLLHGHSQPLQNLQPQLRSFFLQEYLHQADPTPNNIQMASRNLTNGLEEYIRESFASVTVRDDVDITRTNLEFLQDQFNRITTHILHCADSTFGQRLLEMCNQSLFEWLALNLYCLRGDQSALTSVINERIRRLSLDVSPVLVSWVTSVLSLRLQVLLGQMPVTEGEIQRHIRRVGDVPQAPEASSQDQPMETTPVDCQQNGAASPVPATTVEEVLFLPPQSSVPTICTDSEHPTQEDTGSEQWAASVPPEWVPVIRQDMQNQRKMKQQPPLSDAYLSGMPAKRRKTMQGEGPHLSLSEAVSRAMKATGAKPESSTDCVRRELDNSEAQGRYREQLCQDIQNILQDNESYSAQRFPNTQRAFRGDP

Target ORF information:

RefSeq Version	XM_012967838.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967838.3

ORF Insert Sequence:

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT CACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTTCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAGTAAGTGG GAATGATGGC TCCACTCTTG ATGTCCATAT AAATCTGGAT 
CAACAGCTCC CTGTCCAGAG TGAACCTCGT GTTCGTCTTG TTTTGGCTCA GCAAATACTG 
CAGGACATAC AGAGGATACT GGATCGTTTG GAGGGACAAC CAGTTAATGA GCAGACTGCA 
GAACCGATGG ACACAGCAGT GTCTGAAGGA GAGGCCAGCA GTAGAGAAAC CTTGCCTCAG 
ACTACACAGA ATACAGATGG GCAGTCGAAT ACAGCCCCTA CCAGTCATCC TTCACCATCA 
GAATATGTTG AGGTCCTGCA GTCTTTGTCA CGGGTTGAGG AACGTCTAGC CCCTTTCATG 
CAACGTTACC GGGAAATCCT TAGTAGTGCT ACCTCTGATG CCTATGAGAA TCAGGAAGAA 
AGGGAGCAGT CCCAGCGGAT AATTAATTTA GTTGGAGAAT CTCTTCGTCT CCTGGGAAAT 
GCCCTGGTTG CTGTATCCGA CTTGCGCTGC AATCTCTCTT CTGCTTCTCC TAGACACTTG 
CATGTTGTTC GCCCTATGTC CCATTACACT GGGCCCATGC TACTACAGCA AGCTGCTATA 
CCAATACAGA TTAATGTTGG TACTACAGTC ACTATGACTG GGAATGGAAC CCATGCTGGT 
CAGATGCCTT CAGATGGGAA TGCTGCTCAT ACTCCTACCA ATACTTCAGA ACCTCAGCGA 
TCTAACTCTG ACAACCAACC TCCATCCAGT GGAGAGAGAC CAGCTTCTGA GATCCCTCCC 
ACTTCAGTTC CACATCCCCA CCCTCGTGTT GTACGGATTA CGCACCAGAC TGTTGAGCCT 
GTCATGATGA TGCATATGAA CATTCAGGAT TCTGGACCTG GTGGCCCTAC AAACATTCCC 
CCACCAACTG CGGGGCATGG GGGAAGTGCA CACATTCATA TGCCAGGATT ACCCCCTGAG 
TTCATGCAGG CAATTTCTCA CCAGATTACA CAGCAAGCTG TGGCTGCAGC TTCAGGGCAG 
CAAATTCCTG GCTTCCAAGC TCCACCACGC TTTGTTTTTA CTAGGCCTGC TGCCCCTTCA 
TTTCCACCCC AGCCTGGGGT AGCAACTACC CCACCAGGAC CTGGAGGGGC AACTACTGCT 
GTACCTGGGG AAACGGTGGG TCCAGCAGGA AATGCCTCCT TGGCTCAGAT GATCAGCGGT 
CTTGTTGGAC AGCTTCTGAT GCACCCAGTT ATTGTTGCTC AGGGTGGCAG CAACACCCCC 
TCTTCTACGT CAACCCCAAC TTCTACATCC TCCTCCAGCT CCTCTTCATC CTCCACTGTC 
ACTACCTCTA CAACAACCAC CTCTTCCACA TCCTTCCCCA CTGTTTCATC AGGTCCCTCT 
CCCCAGCCCC CACCTGGCAC TGATCAACAC CTCTCTCAGC TCCTGGGCTC TTTACTGGGA 
ACTGCCGGCT CAGGGATGTC AAACTTCGCT ATGGGTTCGC CGAGTATTAC TGTTACCGTG 
CCTGGAATGC CGGCCTTTTT ACAGGGTGTC ACAGATATAT TACAGGCTAC ACAGACTGTT 
CCTGTTTCTA CTTCTCCTCC CCAGTCTGCT TCTCAGGCTC CTCCTCCTTC ATCACCCTCA 
CCACCTCCTG CCCATTCCTC TCCCCCTCCA GCTGCCGCAC CAGAGTCTCT TCCTCCAGAG 
TTTTTCACTA GTGTTGTTCA GGGTGTCCTT TCATCCATGC TGGGGTCTTT GAGTGCAGCT 
GATCAGAGTG GAACAGAAAG CATTGCAGCT TTCATTCAGA GGCTCAGTGG CTCCCACAAC 
ATTTTCCAGC CTGATGCAGA GGGACCTGGT GGATTTTTTG GTGACTTACT CACCTTGATT 
TGTCACAATT TCTCCCTGGT CGATATGGTA ATGTTACTGC ATGGTCATTC CCAGCCTTTG 
CAGAATCTGC AGCCTCAACT GAGATCCTTC TTTCTTCAGG AGTATCTACA CCAGGCAGAT 
CCCACACCTA ATAACATCCA GATGGCATCA AGAAATCTTA CTAATGGCCT GGAAGAGTAC 
ATAAGAGAAA GCTTTGCCTC CGTTACTGTG CGTGATGATG TTGATATCAC AAGAACAAAC 
CTTGAATTTC TACAGGATCA GTTCAACAGG ATCACAACAC ACATCCTACA TTGTGCAGAT 
TCCACATTCG GACAGCGGCT TCTTGAGATG TGTAACCAGT CTCTCTTTGA ATGGCTTGCT 
CTCAACCTGT ATTGCCTCCG AGGGGACCAG AGTGCTCTCA CATCTGTCAT TAATGAGAGA 
ATTCGAAGAC TCTCTCTAGA TGTTTCACCT GTGCTTGTCT CGTGGGTGAC GTCTGTCCTG 
TCCCTCCGTC TTCAAGTGCT CTTGGGGCAG ATGCCTGTGA CTGAGGGCGA GATACAGAGA 
CATATACGGA GAGTGGGAGA TGTTCCGCAG GCTCCTGAAG CATCATCTCA GGATCAACCT 
ATGGAAACCA CACCTGTTGA TTGTCAGCAG AATGGTGCTG CCTCTCCAGT TCCTGCCACC 
ACAGTGGAGG AGGTGCTTTT CCTCCCCCCT CAAAGTTCAG TACCCACCAT ATGCACAGAT 
TCTGAGCACC CAACCCAAGA AGATACCGGA TCTGAGCAAT GGGCAGCATC TGTGCCCCCA 
GAATGGGTTC CTGTAATCAG ACAAGATATG CAGAACCAGC GGAAGATGAA ACAGCAGCCA 
CCCTTGAGTG ATGCCTACCT GAGTGGCATG CCTGCAAAGC GCCGCAAAAC AATGCAAGGT 
GAAGGGCCAC ACTTGTCCCT CTCTGAAGCT GTGAGCCGTG CAATGAAAGC CACAGGAGCC 
AAACCAGAGA GCAGTACAGA CTGTGTTAGG AGGGAGCTGG ATAACAGTGA AGCACAAGGA 
AGGTACAGAG AACAGTTGTG TCAGGATATC CAGAACATCC TCCAAGACAA TGAGTCCTAC 
AGTGCCCAGA GATTCCCCAA CACACAAAGG GCATTCAGAG GGGACCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bag6 ( XM_018096092.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951581.2 Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X2, mRNA.

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CloneID	OXa63368
Clone ID Related Accession (Same CDS sequence)	XM_018096092.2
Accession Version	XM_018096092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	large proline-rich protein bag6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT CACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTTCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAAATGATGG CTCCACTCTT GATGTCCATA TAAATCTGGA TCAACAGCTC 
CCTGTCCAGA GTGAACCTCG TGTTCGTCTT GTTTTGGCTC AGCAAATACT GCAGGACATA 
CAGAGGATAC TGGATCGTTT GGAGGGACAA CCAGTTAATG AGCAGACTGC AGAACCGATG 
GACACAGCAG TGTCTGAAGG AGAGGCCAGC AGTAGAGAAA CCTTGCCTCA GACTACACAG 
AATACAGATG GGCAGTCGAA TACAGCCCCT ACCAGTCATC CTTCACCATC AGAATATGTT 
GAGGTCCTGC AGTCTTTGTC ACGGGTTGAG GAACGTCTAG CCCCTTTCAT GCAACGTTAC 
CGGGAAATCC TTAGTAGTGC TACCTCTGAT GCCTATGAGA ATCAGGAAGA AAGGGAGCAG 
TCCCAGCGGA TAATTAATTT AGTTGGAGAA TCTCTTCGTC TCCTGGGAAA TGCCCTGGTT 
GCTGTATCCG ACTTGCGCTG CAATCTCTCT TCTGCTTCTC CTAGACACTT GCATGTTGTT 
CGCCCTATGT CCCATTACAC TGGGCCCATG CTACTACAGC AAGCTGCTAT ACCAATACAG 
ATTAATGTTG GTACTACAGT CACTATGACT GGGAATGGAA CCCATGCTGG TCAGATGCCT 
TCAGATGGGA ATGCTGCTCA TACTCCTACC AATACTTCAG AACCTCAGCG ATCTAACTCT 
GACAACCAAC CTCCATCCAG TGGAGAGAGA CCAGCTTCTG AGATCCCTCC CACTTCAGTT 
CCACATCCCC ACCCTCGTGT TGTACGGATT ACGCACCAGA CTGTTGAGCC TGTCATGATG 
ATGCATATGA ACATTCAGGA TTCTGGACCT GGTGGCCCTA CAAACATTCC CCCACCAACT 
GCGGGGCATG GGGGAAGTGC ACACATTCAT ATGCCAGGAT TACCCCCTGA GTTCATGCAG 
GCAATTTCTC ACCAGATTAC ACAGCAAGCT GTGGCTGCAG CTTCAGGGCA GCAAATTCCT 
GGCTTCCAAG CTCCACCACG CTTTGTTTTT ACTAGGCCTG CTGCCCCTTC ATTTCCACCC 
CAGCCTGGGG TAGCAACTAC CCCACCAGGA CCTGGAGGGG CAACTACTGC TGTACCTGGG 
GAAACGGTGG GTCCAGCAGG AAATGCCTCC TTGGCTCAGA TGATCAGCGG TCTTGTTGGA 
CAGCTTCTGA TGCACCCAGT TATTGTTGCT CAGGGTGGCA GCAACACCCC CTCTTCTACG 
TCAACCCCAA CTTCTACATC CTCCTCCAGC TCCTCTTCAT CCTCCACTGT CACTACCTCT 
ACAACAACCA CCTCTTCCAC ATCCTTCCCC ACTGTTTCAT CAGGTCCCTC TCCCCAGCCC 
CCACCTGGCA CTGATCAACA CCTCTCTCAG CTCCTGGGCT CTTTACTGGG AACTGCCGGC 
TCAGGGATGT CAAACTTCGC TATGGGTTCG CCGAGTATTA CTGTTACCGT GCCTGGAATG 
CCGGCCTTTT TACAGGGTGT CACAGATATA TTACAGGCTA CACAGACTGT TCCTGTTTCT 
ACTTCTCCTC CCCAGTCTGC TTCTCAGGCT CCTCCTCCTT CATCACCCTC ACCACCTCCT 
GCCCATTCCT CTCCCCCTCC AGCTGCCGCA CCAGAGTCTC TTCCTCCAGA GTTTTTCACT 
AGTGTTGTTC AGGGTGTCCT TTCATCCATG CTGGGGTCTT TGAGTGCAGC TGATCAGAGT 
GGAACAGAAA GCATTGCAGC TTTCATTCAG AGGCTCAGTG GCTCCCACAA CATTTTCCAG 
CCTGATGCAG AGGGACCTGG TGGATTTTTT GGTGACTTAC TCACCTTGAT TTGTCACAAT 
TTCTCCCTGG TCGATATGGT AATGTTACTG CATGGTCATT CCCAGCCTTT GCAGAATCTG 
CAGCCTCAAC TGAGATCCTT CTTTCTTCAG GAGTATCTAC ACCAGGCAGA TCCCACACCT 
AATAACATCC AGATGGCATC AAGAAATCTT ACTAATGGCC TGGAAGAGTA CATAAGAGAA 
AGCTTTGCCT CCGTTACTGT GCGTGATGAT GTTGATATCA CAAGAACAAA CCTTGAATTT 
CTACAGGATC AGTTCAACAG GATCACAACA CACATCCTAC ATTGTGCAGA TTCCACATTC 
GGACAGCGGC TTCTTGAGAT GTGTAACCAG TCTCTCTTTG AATGGCTTGC TCTCAACCTG 
TATTGCCTCC GAGGGGACCA GAGTGCTCTC ACATCTGTCA TTAATGAGAG AATTCGAAGA 
CTCTCTCTAG ATGTTTCACC TGTGCTTGTC TCGTGGGTGA CGTCTGTCCT GTCCCTCCGT 
CTTCAAGTGC TCTTGGGGCA GATGCCTGTG ACTGAGGGCG AGATACAGAG ACATATACGG 
AGAGTGGGAG ATGTTCCGCA GGCTCCTGAA GCATCATCTC AGGATCAACC TATGGAAACC 
ACACCTGTTG ATTGTCAGCA GAATGGTGCT GCCTCTCCAG TTCCTGCCAC CACAGTGGAG 
GAGGTGCTTT TCCTCCCCCC TCAAAGTTCA GTACCCACCA TATGCACAGA TTCTGAGCAC 
CCAACCCAAG AAGATACCGG ATCTGAGCAA TGGGCAGCAT CTGTGCCCCC AGAATGGGTT 
CCTGTAATCA GACAAGATAT GCAGAACCAG CGGAAGATGA AACAGCAGCC ACCCTTGAGT 
GATGCCTACC TGAGTGGCAT GCCTGCAAAG CGCCGCAAAA CAATGCAAGG TGAAGGGCCA 
CACTTGTCCC TCTCTGAAGC TGTGAGCCGT GCAATGAAAG CCACAGGAGC CAAACCAGAG 
AGCAGTACAG ACTGTGTTAG GAGGGAGCTG GATAACAGTG AAGCACAAGG AAGGTACAGA 
GAACAGTTGT GTCAGGATAT CCAGAACATC CTCCAAGACA ATGAGTCCTA CAGTGCCCAG 
AGATTCCCCA ACACACAAAG GGCATTCAGA GGGGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951581.2
CDS	191..3592
Translation	MAANEKMEVTVKTLDSQTRTFTVDAEITVKEFKTHISSDVGISPEKQRLIYQGRVLQEDKKLKEYNVDGKVIHLVERAPPQTQPSTGGPSTSSSTSPSSSNAANVPGAGAPERNGNSYVMVGTFNLPHVMSGLGEASRGPRVSTNDGSTLDVHINLDQQLPVQSEPRVRLVLAQQILQDIQRILDRLEGQPVNEQTAEPMDTAVSEGEASSRETLPQTTQNTDGQSNTAPTSHPSPSEYVEVLQSLSRVEERLAPFMQRYREILSSATSDAYENQEEREQSQRIINLVGESLRLLGNALVAVSDLRCNLSSASPRHLHVVRPMSHYTGPMLLQQAAIPIQINVGTTVTMTGNGTHAGQMPSDGNAAHTPTNTSEPQRSNSDNQPPSSGERPASEIPPTSVPHPHPRVVRITHQTVEPVMMMHMNIQDSGPGGPTNIPPPTAGHGGSAHIHMPGLPPEFMQAISHQITQQAVAAASGQQIPGFQAPPRFVFTRPAAPSFPPQPGVATTPPGPGGATTAVPGETVGPAGNASLAQMISGLVGQLLMHPVIVAQGGSNTPSSTSTPTSTSSSSSSSSSTVTTSTTTTSSTSFPTVSSGPSPQPPPGTDQHLSQLLGSLLGTAGSGMSNFAMGSPSITVTVPGMPAFLQGVTDILQATQTVPVSTSPPQSASQAPPPSSPSPPPAHSSPPPAAAPESLPPEFFTSVVQGVLSSMLGSLSAADQSGTESIAAFIQRLSGSHNIFQPDAEGPGGFFGDLLTLICHNFSLVDMVMLLHGHSQPLQNLQPQLRSFFLQEYLHQADPTPNNIQMASRNLTNGLEEYIRESFASVTVRDDVDITRTNLEFLQDQFNRITTHILHCADSTFGQRLLEMCNQSLFEWLALNLYCLRGDQSALTSVINERIRRLSLDVSPVLVSWVTSVLSLRLQVLLGQMPVTEGEIQRHIRRVGDVPQAPEASSQDQPMETTPVDCQQNGAASPVPATTVEEVLFLPPQSSVPTICTDSEHPTQEDTGSEQWAASVPPEWVPVIRQDMQNQRKMKQQPPLSDAYLSGMPAKRRKTMQGEGPHLSLSEAVSRAMKATGAKPESSTDCVRRELDNSEAQGRYREQLCQDIQNILQDNESYSAQRFPNTQRAFRGDP

Target ORF information:

RefSeq Version	XM_018096092.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096092.2

ORF Insert Sequence:

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT CACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTTCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAAATGATGG CTCCACTCTT GATGTCCATA TAAATCTGGA TCAACAGCTC 
CCTGTCCAGA GTGAACCTCG TGTTCGTCTT GTTTTGGCTC AGCAAATACT GCAGGACATA 
CAGAGGATAC TGGATCGTTT GGAGGGACAA CCAGTTAATG AGCAGACTGC AGAACCGATG 
GACACAGCAG TGTCTGAAGG AGAGGCCAGC AGTAGAGAAA CCTTGCCTCA GACTACACAG 
AATACAGATG GGCAGTCGAA TACAGCCCCT ACCAGTCATC CTTCACCATC AGAATATGTT 
GAGGTCCTGC AGTCTTTGTC ACGGGTTGAG GAACGTCTAG CCCCTTTCAT GCAACGTTAC 
CGGGAAATCC TTAGTAGTGC TACCTCTGAT GCCTATGAGA ATCAGGAAGA AAGGGAGCAG 
TCCCAGCGGA TAATTAATTT AGTTGGAGAA TCTCTTCGTC TCCTGGGAAA TGCCCTGGTT 
GCTGTATCCG ACTTGCGCTG CAATCTCTCT TCTGCTTCTC CTAGACACTT GCATGTTGTT 
CGCCCTATGT CCCATTACAC TGGGCCCATG CTACTACAGC AAGCTGCTAT ACCAATACAG 
ATTAATGTTG GTACTACAGT CACTATGACT GGGAATGGAA CCCATGCTGG TCAGATGCCT 
TCAGATGGGA ATGCTGCTCA TACTCCTACC AATACTTCAG AACCTCAGCG ATCTAACTCT 
GACAACCAAC CTCCATCCAG TGGAGAGAGA CCAGCTTCTG AGATCCCTCC CACTTCAGTT 
CCACATCCCC ACCCTCGTGT TGTACGGATT ACGCACCAGA CTGTTGAGCC TGTCATGATG 
ATGCATATGA ACATTCAGGA TTCTGGACCT GGTGGCCCTA CAAACATTCC CCCACCAACT 
GCGGGGCATG GGGGAAGTGC ACACATTCAT ATGCCAGGAT TACCCCCTGA GTTCATGCAG 
GCAATTTCTC ACCAGATTAC ACAGCAAGCT GTGGCTGCAG CTTCAGGGCA GCAAATTCCT 
GGCTTCCAAG CTCCACCACG CTTTGTTTTT ACTAGGCCTG CTGCCCCTTC ATTTCCACCC 
CAGCCTGGGG TAGCAACTAC CCCACCAGGA CCTGGAGGGG CAACTACTGC TGTACCTGGG 
GAAACGGTGG GTCCAGCAGG AAATGCCTCC TTGGCTCAGA TGATCAGCGG TCTTGTTGGA 
CAGCTTCTGA TGCACCCAGT TATTGTTGCT CAGGGTGGCA GCAACACCCC CTCTTCTACG 
TCAACCCCAA CTTCTACATC CTCCTCCAGC TCCTCTTCAT CCTCCACTGT CACTACCTCT 
ACAACAACCA CCTCTTCCAC ATCCTTCCCC ACTGTTTCAT CAGGTCCCTC TCCCCAGCCC 
CCACCTGGCA CTGATCAACA CCTCTCTCAG CTCCTGGGCT CTTTACTGGG AACTGCCGGC 
TCAGGGATGT CAAACTTCGC TATGGGTTCG CCGAGTATTA CTGTTACCGT GCCTGGAATG 
CCGGCCTTTT TACAGGGTGT CACAGATATA TTACAGGCTA CACAGACTGT TCCTGTTTCT 
ACTTCTCCTC CCCAGTCTGC TTCTCAGGCT CCTCCTCCTT CATCACCCTC ACCACCTCCT 
GCCCATTCCT CTCCCCCTCC AGCTGCCGCA CCAGAGTCTC TTCCTCCAGA GTTTTTCACT 
AGTGTTGTTC AGGGTGTCCT TTCATCCATG CTGGGGTCTT TGAGTGCAGC TGATCAGAGT 
GGAACAGAAA GCATTGCAGC TTTCATTCAG AGGCTCAGTG GCTCCCACAA CATTTTCCAG 
CCTGATGCAG AGGGACCTGG TGGATTTTTT GGTGACTTAC TCACCTTGAT TTGTCACAAT 
TTCTCCCTGG TCGATATGGT AATGTTACTG CATGGTCATT CCCAGCCTTT GCAGAATCTG 
CAGCCTCAAC TGAGATCCTT CTTTCTTCAG GAGTATCTAC ACCAGGCAGA TCCCACACCT 
AATAACATCC AGATGGCATC AAGAAATCTT ACTAATGGCC TGGAAGAGTA CATAAGAGAA 
AGCTTTGCCT CCGTTACTGT GCGTGATGAT GTTGATATCA CAAGAACAAA CCTTGAATTT 
CTACAGGATC AGTTCAACAG GATCACAACA CACATCCTAC ATTGTGCAGA TTCCACATTC 
GGACAGCGGC TTCTTGAGAT GTGTAACCAG TCTCTCTTTG AATGGCTTGC TCTCAACCTG 
TATTGCCTCC GAGGGGACCA GAGTGCTCTC ACATCTGTCA TTAATGAGAG AATTCGAAGA 
CTCTCTCTAG ATGTTTCACC TGTGCTTGTC TCGTGGGTGA CGTCTGTCCT GTCCCTCCGT 
CTTCAAGTGC TCTTGGGGCA GATGCCTGTG ACTGAGGGCG AGATACAGAG ACATATACGG 
AGAGTGGGAG ATGTTCCGCA GGCTCCTGAA GCATCATCTC AGGATCAACC TATGGAAACC 
ACACCTGTTG ATTGTCAGCA GAATGGTGCT GCCTCTCCAG TTCCTGCCAC CACAGTGGAG 
GAGGTGCTTT TCCTCCCCCC TCAAAGTTCA GTACCCACCA TATGCACAGA TTCTGAGCAC 
CCAACCCAAG AAGATACCGG ATCTGAGCAA TGGGCAGCAT CTGTGCCCCC AGAATGGGTT 
CCTGTAATCA GACAAGATAT GCAGAACCAG CGGAAGATGA AACAGCAGCC ACCCTTGAGT 
GATGCCTACC TGAGTGGCAT GCCTGCAAAG CGCCGCAAAA CAATGCAAGG TGAAGGGCCA 
CACTTGTCCC TCTCTGAAGC TGTGAGCCGT GCAATGAAAG CCACAGGAGC CAAACCAGAG 
AGCAGTACAG ACTGTGTTAG GAGGGAGCTG GATAACAGTG AAGCACAAGG AAGGTACAGA 
GAACAGTTGT GTCAGGATAT CCAGAACATC CTCCAAGACA ATGAGTCCTA CAGTGCCCAG 
AGATTCCCCA ACACACAAAG GGCATTCAGA GGGGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bag6 ( XM_012967839.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823293.2 Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X3, mRNA.

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CloneID	OXa63369
Clone ID Related Accession (Same CDS sequence)	XM_012967839.3
Accession Version	XM_012967839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	large proline-rich protein bag6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT CACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTTCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAAGTGAACC TCGTGTTCGT CTTGTTTTGG CTCAGCAAAT ACTGCAGGAC 
ATACAGAGGA TACTGGATCG TTTGGAGGGA CAACCAGTTA ATGAGCAGAC TGCAGAACCG 
ATGGACACAG CAGTGTCTGA AGGAGAGGCC AGCAGTAGAG AAACCTTGCC TCAGACTACA 
CAGAATACAG ATGGGCAGTC GAATACAGCC CCTACCAGTC ATCCTTCACC ATCAGAATAT 
GTTGAGGTCC TGCAGTCTTT GTCACGGGTT GAGGAACGTC TAGCCCCTTT CATGCAACGT 
TACCGGGAAA TCCTTAGTAG TGCTACCTCT GATGCCTATG AGAATCAGGA AGAAAGGGAG 
CAGTCCCAGC GGATAATTAA TTTAGTTGGA GAATCTCTTC GTCTCCTGGG AAATGCCCTG 
GTTGCTGTAT CCGACTTGCG CTGCAATCTC TCTTCTGCTT CTCCTAGACA CTTGCATGTT 
GTTCGCCCTA TGTCCCATTA CACTGGGCCC ATGCTACTAC AGCAAGCTGC TATACCAATA 
CAGATTAATG TTGGTACTAC AGTCACTATG ACTGGGAATG GAACCCATGC TGGTCAGATG 
CCTTCAGATG GGAATGCTGC TCATACTCCT ACCAATACTT CAGAACCTCA GCGATCTAAC 
TCTGACAACC AACCTCCATC CAGTGGAGAG AGACCAGCTT CTGAGATCCC TCCCACTTCA 
GTTCCACATC CCCACCCTCG TGTTGTACGG ATTACGCACC AGACTGTTGA GCCTGTCATG 
ATGATGCATA TGAACATTCA GGATTCTGGA CCTGGTGGCC CTACAAACAT TCCCCCACCA 
ACTGCGGGGC ATGGGGGAAG TGCACACATT CATATGCCAG GATTACCCCC TGAGTTCATG 
CAGGCAATTT CTCACCAGAT TACACAGCAA GCTGTGGCTG CAGCTTCAGG GCAGCAAATT 
CCTGGCTTCC AAGCTCCACC ACGCTTTGTT TTTACTAGGC CTGCTGCCCC TTCATTTCCA 
CCCCAGCCTG GGGTAGCAAC TACCCCACCA GGACCTGGAG GGGCAACTAC TGCTGTACCT 
GGGGAAACGG TGGGTCCAGC AGGAAATGCC TCCTTGGCTC AGATGATCAG CGGTCTTGTT 
GGACAGCTTC TGATGCACCC AGTTATTGTT GCTCAGGGTG GCAGCAACAC CCCCTCTTCT 
ACGTCAACCC CAACTTCTAC ATCCTCCTCC AGCTCCTCTT CATCCTCCAC TGTCACTACC 
TCTACAACAA CCACCTCTTC CACATCCTTC CCCACTGTTT CATCAGGTCC CTCTCCCCAG 
CCCCCACCTG GCACTGATCA ACACCTCTCT CAGCTCCTGG GCTCTTTACT GGGAACTGCC 
GGCTCAGGGA TGTCAAACTT CGCTATGGGT TCGCCGAGTA TTACTGTTAC CGTGCCTGGA 
ATGCCGGCCT TTTTACAGGG TGTCACAGAT ATATTACAGG CTACACAGAC TGTTCCTGTT 
TCTACTTCTC CTCCCCAGTC TGCTTCTCAG GCTCCTCCTC CTTCATCACC CTCACCACCT 
CCTGCCCATT CCTCTCCCCC TCCAGCTGCC GCACCAGAGT CTCTTCCTCC AGAGTTTTTC 
ACTAGTGTTG TTCAGGGTGT CCTTTCATCC ATGCTGGGGT CTTTGAGTGC AGCTGATCAG 
AGTGGAACAG AAAGCATTGC AGCTTTCATT CAGAGGCTCA GTGGCTCCCA CAACATTTTC 
CAGCCTGATG CAGAGGGACC TGGTGGATTT TTTGGTGACT TACTCACCTT GATTTGTCAC 
AATTTCTCCC TGGTCGATAT GGTAATGTTA CTGCATGGTC ATTCCCAGCC TTTGCAGAAT 
CTGCAGCCTC AACTGAGATC CTTCTTTCTT CAGGAGTATC TACACCAGGC AGATCCCACA 
CCTAATAACA TCCAGATGGC ATCAAGAAAT CTTACTAATG GCCTGGAAGA GTACATAAGA 
GAAAGCTTTG CCTCCGTTAC TGTGCGTGAT GATGTTGATA TCACAAGAAC AAACCTTGAA 
TTTCTACAGG ATCAGTTCAA CAGGATCACA ACACACATCC TACATTGTGC AGATTCCACA 
TTCGGACAGC GGCTTCTTGA GATGTGTAAC CAGTCTCTCT TTGAATGGCT TGCTCTCAAC 
CTGTATTGCC TCCGAGGGGA CCAGAGTGCT CTCACATCTG TCATTAATGA GAGAATTCGA 
AGACTCTCTC TAGATGTTTC ACCTGTGCTT GTCTCGTGGG TGACGTCTGT CCTGTCCCTC 
CGTCTTCAAG TGCTCTTGGG GCAGATGCCT GTGACTGAGG GCGAGATACA GAGACATATA 
CGGAGAGTGG GAGATGTTCC GCAGGCTCCT GAAGCATCAT CTCAGGATCA ACCTATGGAA 
ACCACACCTG TTGATTGTCA GCAGAATGGT GCTGCCTCTC CAGTTCCTGC CACCACAGTG 
GAGGAGGTGC TTTTCCTCCC CCCTCAAAGT TCAGTACCCA CCATATGCAC AGATTCTGAG 
CACCCAACCC AAGAAGATAC CGGATCTGAG CAATGGGCAG CATCTGTGCC CCCAGAATGG 
GTTCCTGTAA TCAGACAAGA TATGCAGAAC CAGCGGAAGA TGAAACAGCA GCCACCCTTG 
AGTGATGCCT ACCTGAGTGG CATGCCTGCA AAGCGCCGCA AAACAATGCA AGGTGAAGGG 
CCACACTTGT CCCTCTCTGA AGCTGTGAGC CGTGCAATGA AAGCCACAGG AGCCAAACCA 
GAGAGCAGTA CAGACTGTGT TAGGAGGGAG CTGGATAACA GTGAAGCACA AGGAAGGTAC 
AGAGAACAGT TGTGTCAGGA TATCCAGAAC ATCCTCCAAG ACAATGAGTC CTACAGTGCC 
CAGAGATTCC CCAACACACA AAGGGCATTC AGAGGGGACC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823293.2
CDS	191..3535
Translation	MAANEKMEVTVKTLDSQTRTFTVDAEITVKEFKTHISSDVGISPEKQRLIYQGRVLQEDKKLKEYNVDGKVIHLVERAPPQTQPSTGGPSTSSSTSPSSSNAANVPGAGAPERNGNSYVMVGTFNLPHVMSGLGEASRGPRVSTSEPRVRLVLAQQILQDIQRILDRLEGQPVNEQTAEPMDTAVSEGEASSRETLPQTTQNTDGQSNTAPTSHPSPSEYVEVLQSLSRVEERLAPFMQRYREILSSATSDAYENQEEREQSQRIINLVGESLRLLGNALVAVSDLRCNLSSASPRHLHVVRPMSHYTGPMLLQQAAIPIQINVGTTVTMTGNGTHAGQMPSDGNAAHTPTNTSEPQRSNSDNQPPSSGERPASEIPPTSVPHPHPRVVRITHQTVEPVMMMHMNIQDSGPGGPTNIPPPTAGHGGSAHIHMPGLPPEFMQAISHQITQQAVAAASGQQIPGFQAPPRFVFTRPAAPSFPPQPGVATTPPGPGGATTAVPGETVGPAGNASLAQMISGLVGQLLMHPVIVAQGGSNTPSSTSTPTSTSSSSSSSSSTVTTSTTTTSSTSFPTVSSGPSPQPPPGTDQHLSQLLGSLLGTAGSGMSNFAMGSPSITVTVPGMPAFLQGVTDILQATQTVPVSTSPPQSASQAPPPSSPSPPPAHSSPPPAAAPESLPPEFFTSVVQGVLSSMLGSLSAADQSGTESIAAFIQRLSGSHNIFQPDAEGPGGFFGDLLTLICHNFSLVDMVMLLHGHSQPLQNLQPQLRSFFLQEYLHQADPTPNNIQMASRNLTNGLEEYIRESFASVTVRDDVDITRTNLEFLQDQFNRITTHILHCADSTFGQRLLEMCNQSLFEWLALNLYCLRGDQSALTSVINERIRRLSLDVSPVLVSWVTSVLSLRLQVLLGQMPVTEGEIQRHIRRVGDVPQAPEASSQDQPMETTPVDCQQNGAASPVPATTVEEVLFLPPQSSVPTICTDSEHPTQEDTGSEQWAASVPPEWVPVIRQDMQNQRKMKQQPPLSDAYLSGMPAKRRKTMQGEGPHLSLSEAVSRAMKATGAKPESSTDCVRRELDNSEAQGRYREQLCQDIQNILQDNESYSAQRFPNTQRAFRGDP

Target ORF information:

RefSeq Version	XM_012967839.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967839.3

ORF Insert Sequence:

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT CACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTTCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAAGTGAACC TCGTGTTCGT CTTGTTTTGG CTCAGCAAAT ACTGCAGGAC 
ATACAGAGGA TACTGGATCG TTTGGAGGGA CAACCAGTTA ATGAGCAGAC TGCAGAACCG 
ATGGACACAG CAGTGTCTGA AGGAGAGGCC AGCAGTAGAG AAACCTTGCC TCAGACTACA 
CAGAATACAG ATGGGCAGTC GAATACAGCC CCTACCAGTC ATCCTTCACC ATCAGAATAT 
GTTGAGGTCC TGCAGTCTTT GTCACGGGTT GAGGAACGTC TAGCCCCTTT CATGCAACGT 
TACCGGGAAA TCCTTAGTAG TGCTACCTCT GATGCCTATG AGAATCAGGA AGAAAGGGAG 
CAGTCCCAGC GGATAATTAA TTTAGTTGGA GAATCTCTTC GTCTCCTGGG AAATGCCCTG 
GTTGCTGTAT CCGACTTGCG CTGCAATCTC TCTTCTGCTT CTCCTAGACA CTTGCATGTT 
GTTCGCCCTA TGTCCCATTA CACTGGGCCC ATGCTACTAC AGCAAGCTGC TATACCAATA 
CAGATTAATG TTGGTACTAC AGTCACTATG ACTGGGAATG GAACCCATGC TGGTCAGATG 
CCTTCAGATG GGAATGCTGC TCATACTCCT ACCAATACTT CAGAACCTCA GCGATCTAAC 
TCTGACAACC AACCTCCATC CAGTGGAGAG AGACCAGCTT CTGAGATCCC TCCCACTTCA 
GTTCCACATC CCCACCCTCG TGTTGTACGG ATTACGCACC AGACTGTTGA GCCTGTCATG 
ATGATGCATA TGAACATTCA GGATTCTGGA CCTGGTGGCC CTACAAACAT TCCCCCACCA 
ACTGCGGGGC ATGGGGGAAG TGCACACATT CATATGCCAG GATTACCCCC TGAGTTCATG 
CAGGCAATTT CTCACCAGAT TACACAGCAA GCTGTGGCTG CAGCTTCAGG GCAGCAAATT 
CCTGGCTTCC AAGCTCCACC ACGCTTTGTT TTTACTAGGC CTGCTGCCCC TTCATTTCCA 
CCCCAGCCTG GGGTAGCAAC TACCCCACCA GGACCTGGAG GGGCAACTAC TGCTGTACCT 
GGGGAAACGG TGGGTCCAGC AGGAAATGCC TCCTTGGCTC AGATGATCAG CGGTCTTGTT 
GGACAGCTTC TGATGCACCC AGTTATTGTT GCTCAGGGTG GCAGCAACAC CCCCTCTTCT 
ACGTCAACCC CAACTTCTAC ATCCTCCTCC AGCTCCTCTT CATCCTCCAC TGTCACTACC 
TCTACAACAA CCACCTCTTC CACATCCTTC CCCACTGTTT CATCAGGTCC CTCTCCCCAG 
CCCCCACCTG GCACTGATCA ACACCTCTCT CAGCTCCTGG GCTCTTTACT GGGAACTGCC 
GGCTCAGGGA TGTCAAACTT CGCTATGGGT TCGCCGAGTA TTACTGTTAC CGTGCCTGGA 
ATGCCGGCCT TTTTACAGGG TGTCACAGAT ATATTACAGG CTACACAGAC TGTTCCTGTT 
TCTACTTCTC CTCCCCAGTC TGCTTCTCAG GCTCCTCCTC CTTCATCACC CTCACCACCT 
CCTGCCCATT CCTCTCCCCC TCCAGCTGCC GCACCAGAGT CTCTTCCTCC AGAGTTTTTC 
ACTAGTGTTG TTCAGGGTGT CCTTTCATCC ATGCTGGGGT CTTTGAGTGC AGCTGATCAG 
AGTGGAACAG AAAGCATTGC AGCTTTCATT CAGAGGCTCA GTGGCTCCCA CAACATTTTC 
CAGCCTGATG CAGAGGGACC TGGTGGATTT TTTGGTGACT TACTCACCTT GATTTGTCAC 
AATTTCTCCC TGGTCGATAT GGTAATGTTA CTGCATGGTC ATTCCCAGCC TTTGCAGAAT 
CTGCAGCCTC AACTGAGATC CTTCTTTCTT CAGGAGTATC TACACCAGGC AGATCCCACA 
CCTAATAACA TCCAGATGGC ATCAAGAAAT CTTACTAATG GCCTGGAAGA GTACATAAGA 
GAAAGCTTTG CCTCCGTTAC TGTGCGTGAT GATGTTGATA TCACAAGAAC AAACCTTGAA 
TTTCTACAGG ATCAGTTCAA CAGGATCACA ACACACATCC TACATTGTGC AGATTCCACA 
TTCGGACAGC GGCTTCTTGA GATGTGTAAC CAGTCTCTCT TTGAATGGCT TGCTCTCAAC 
CTGTATTGCC TCCGAGGGGA CCAGAGTGCT CTCACATCTG TCATTAATGA GAGAATTCGA 
AGACTCTCTC TAGATGTTTC ACCTGTGCTT GTCTCGTGGG TGACGTCTGT CCTGTCCCTC 
CGTCTTCAAG TGCTCTTGGG GCAGATGCCT GTGACTGAGG GCGAGATACA GAGACATATA 
CGGAGAGTGG GAGATGTTCC GCAGGCTCCT GAAGCATCAT CTCAGGATCA ACCTATGGAA 
ACCACACCTG TTGATTGTCA GCAGAATGGT GCTGCCTCTC CAGTTCCTGC CACCACAGTG 
GAGGAGGTGC TTTTCCTCCC CCCTCAAAGT TCAGTACCCA CCATATGCAC AGATTCTGAG 
CACCCAACCC AAGAAGATAC CGGATCTGAG CAATGGGCAG CATCTGTGCC CCCAGAATGG 
GTTCCTGTAA TCAGACAAGA TATGCAGAAC CAGCGGAAGA TGAAACAGCA GCCACCCTTG 
AGTGATGCCT ACCTGAGTGG CATGCCTGCA AAGCGCCGCA AAACAATGCA AGGTGAAGGG 
CCACACTTGT CCCTCTCTGA AGCTGTGAGC CGTGCAATGA AAGCCACAGG AGCCAAACCA 
GAGAGCAGTA CAGACTGTGT TAGGAGGGAG CTGGATAACA GTGAAGCACA AGGAAGGTAC 
AGAGAACAGT TGTGTCAGGA TATCCAGAAC ATCCTCCAAG ACAATGAGTC CTACAGTGCC 
CAGAGATTCC CCAACACACA AAGGGCATTC AGAGGGGACC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bag6 ( NM_001097409.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090878.2 Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant 1, mRNA.

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CloneID	OXa02170
Clone ID Related Accession (Same CDS sequence)	NM_001097409.2
Accession Version	NM_001097409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	large proline-rich protein bag6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT485713.1, BC135335.1, DT413979.1, DT439387.1 and CX913235.2. On Oct 7, 2011 this sequence version replaced NM_001097409.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC135335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAGTAAGTGG GAATGATGGC TCCACTCTTG ATGTCCATAT AAATCTGGAT 
CAACAGCTCC CTGTCCAGAG TGAACCTCGT GTTCGTCTTG TTTTGGCTCA GCAAATACTG 
CAGGACATAC AGAGGATACT GGATCGTTTG GAGGGACAAC CAGTTAATGA GCAGACTGCA 
GAACCGATGG ACACAGCAGT GTCTGAAGGA GAGGCCAGCA GTAGAGAAAC CTTGCCTCAG 
ACTACACAGA ATACAGATGG GCAGTCGAAT ACAGCCCCTA CCAGTCATCC TTCACCATCA 
GAATATGTTG AGGTCCTGCA GTCTTTGTCA CGGGTTGAGG AACGTCTAGC CCCTTTCATG 
CAACGTTACC GGGAAATCCT TAGTAGTGCT ACCTCTGATG CCTATGAGAA TCAGGAAGAA 
AGGGAGCAGT CCCAGCGGAT AATTAATTTA GTTGGAGAAT CTCTTCGTCT CCTGGGAAAT 
GCCCTGGTTG CTGTATCCGA CTTGCGCTGC AATCTCTCTT CTGCTTCTCC CAGACACTTG 
CATGTTGTTC GCCCAATGTC CCATTACACT GGGCCCATGC TACTACAGCA AGCTGCTATA 
CCAATACAGA TTAATGTTGG TACTACAGTC ACTATGACTG GGAATGGAAC CCATGCTGGT 
CAGATGCCTT CAGATGGGAA TGCTGCTCAT ACTCCTACCA ATACTTCAGA ACCTCAGCGA 
TCTAACTCTG ACAACCAACC TCCATCCAGT GGAGAGAGAC CAGCTTCTGA GATCCCTCCC 
ACTTCAGTTC CACATCCCCA CCCTCGTGTT GTACGGATTA CGCACCAGAC TGTTGAGCCT 
GTCATGATGA TGCATATGAA CATTCAGGAT TCTGGACCTG GTGGCCCTAC AAACGTTCCC 
CCACCAACTG CGGGGCATGG GGGAAGTGCA CACATTCATA TGCCAGGATT ACCCCCTGAG 
TTCATGCAGG CAATTTCTCA CCAGATTACA CAGCAAGCTG TGGCTGCAGC TTCAGGGCAG 
CAAATTCCTG GCTTCCAAGC TCCACCACGC TTTGTTTTTA CTAGGCCTGC TGCCCCTTCA 
TTTCCACCCC AGCCTGGGGT AGCAACTACC CCACCAGGAC CTGGAGGGGC AACTACTGCT 
GTACCTGGGG CAACGGTGGG TCCAGCAGGA AATGCCTCCT TGGCTCAGAT GATCAGCGGT 
CTTGTTGGAC AGCTTCTGAT GCACCCAGTT ATTGTTGCTC AGGGTGGCAG CAACACCCCC 
TCTTCTACGT CAACCCCAAC TTCTACATCC TCCTCCAGCT CCTCTTCATC CTCCACTGTC 
ACTACCTCTA CAACAACCAC CTCTTCCACA TCCTTCCCCA CTGTTTCATC AGGTCCCTCT 
CCCCAGCCCC CACCTGGCAC TGATCAACAC CTCTCTCAGC TCCTGGGCTC TTTACTGGGA 
ACTGCCGGCT CAGGGATGTC AAACTTCGCT ATGGGTTCGC CGAGTATTAC TGTTACCGTG 
CCTGGAATGC CGGCCTTTTT ACAGGGTGTC ACAGATATAT TACAGGCTAC ACAGACTGTT 
CCTGTTTCTA CTTCTCCTCC CCAGTCTGCT TCTCAGGCTC CTCCTCCTTC ATCACCCTCA 
CCACCTCCTG CCCATTCCTC TCCCCCTCCA GCTGCCGCAC CAGAGTCTCT TCCTCCAGAG 
TTTTTCACTA GTGTTGTTCA GGGTGTCCTT TCATCCATGC TGGGGTCTTT GAGTGCAGCT 
GATCAGAGTG GAACAGAAAG CATTGCAGCT TTCATTCAGA GGCTCAGTGG CTCCCACAAC 
ATTTTCCAGC CTGATGCAGA GGGACCTGGT GGATTTTTTG GTGACTTACT CACCTTGATT 
TGTCACAATT TCTCCCTGGT CGATATGGTA ATGTTACTGC ATGGTCATTC CCAGCCTTTG 
CAGAATCTGC AGCCTCAACT GAGATCCTTC TTTCTTCAGG AGTATCTACA CCAGGCAGAT 
CCCACACCTA ATAACATCCA GATGGCATCA AGAAATCTTA CTAATGGCCT GGAAGAGTAC 
ATAAGAGAAA GCTTTGCCTC CGTTACTGTG CGTGATGATG TTGATATCAC AAGAACAAAC 
CTTGAATTTC TACAGGATCA GTTCAACAGG ATCACAACAC ACATCCTACA TTGTGCAGAT 
TCCACATTCG GACAGCGGCT TCTTGAGATG TGTAACCAGT CTCTCTTTGA ATGGCTTGCT 
CTCAACCTGT ATTGCCTCCG AGGGGACCAG AGTGCTCTCA CATCTGTCAT TAATGAGAGA 
ATTCGAAGAC TCTCTCTAGA TGTTTCACCT GTGCTTGTCT CGTGGGTGAC GTCTGTCCTG 
TCCCTCCGTC TTCAAGTGCT GTTGGGGCAG ATGCCTGTGA CTGAGGGCGA GATACAGAGA 
CATATACGGA GAGTGGGAGA TGTTCCGCAG GCTCCTGAAG CATCATCTCA GGATCAACCT 
ATGGAAACCA CACCTGTTGA TTGTCAGAAT GGTGCTGCCT CTCCAGTTCC TGCCACCACA 
GTGGAGGAGG TGCTTTTCCT CCCCCCTCAA AGTTCAGTAC CCACCATATG CACAGATTCT 
GAGCACCCAA CCCAAGAAGA TACCGGATCT GAGCAATGGG CAGCATCTGT GCCCCCAGAA 
TGGGTTCCTG TAATCAGACA AGATATGCAG AACCAGCGGA AGATGAAACA GCAGCCACCC 
TTGAGTGATG CCTACCTGAG TGGCATGCCT GCAAAGCGCC GCAAAACAAT GCAAGGTGAA 
GGGCCACACT TGTCCCTCTC TGAAGCTGTG AGCCGTGCAA TGAAAGCCAC AGGAGCCAAA 
CCAGAGAGCA GTACAGACTG TGTTAGGAGG GAGCTGGATA ACAGTGAAGC ACAAGGAAGG 
TACAGAGAAC AGTTGTGTCA GGATATCCAG AACATCCTCC AAGACAATGA GTCCTACAGT 
GCCCAGAGAT TCCCCAACAC ACAAAGGGCA TTCAGAGGGG ACCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090878.2
CDS	107..3514
Translation	MAANEKMEVTVKTLDSQTRTFTVDAEITVKEFKTHISSDVGISPEKQRLIYQGRVLQEDKKLKEYNVDGKVIHLVERAPPQTQPPTGGPSTSSSTSPSSSNAANVPGAGAPERNGNSYVMVGTFNLPHVMSGLGEASRGPRVSTVSGNDGSTLDVHINLDQQLPVQSEPRVRLVLAQQILQDIQRILDRLEGQPVNEQTAEPMDTAVSEGEASSRETLPQTTQNTDGQSNTAPTSHPSPSEYVEVLQSLSRVEERLAPFMQRYREILSSATSDAYENQEEREQSQRIINLVGESLRLLGNALVAVSDLRCNLSSASPRHLHVVRPMSHYTGPMLLQQAAIPIQINVGTTVTMTGNGTHAGQMPSDGNAAHTPTNTSEPQRSNSDNQPPSSGERPASEIPPTSVPHPHPRVVRITHQTVEPVMMMHMNIQDSGPGGPTNVPPPTAGHGGSAHIHMPGLPPEFMQAISHQITQQAVAAASGQQIPGFQAPPRFVFTRPAAPSFPPQPGVATTPPGPGGATTAVPGATVGPAGNASLAQMISGLVGQLLMHPVIVAQGGSNTPSSTSTPTSTSSSSSSSSSTVTTSTTTTSSTSFPTVSSGPSPQPPPGTDQHLSQLLGSLLGTAGSGMSNFAMGSPSITVTVPGMPAFLQGVTDILQATQTVPVSTSPPQSASQAPPPSSPSPPPAHSSPPPAAAPESLPPEFFTSVVQGVLSSMLGSLSAADQSGTESIAAFIQRLSGSHNIFQPDAEGPGGFFGDLLTLICHNFSLVDMVMLLHGHSQPLQNLQPQLRSFFLQEYLHQADPTPNNIQMASRNLTNGLEEYIRESFASVTVRDDVDITRTNLEFLQDQFNRITTHILHCADSTFGQRLLEMCNQSLFEWLALNLYCLRGDQSALTSVINERIRRLSLDVSPVLVSWVTSVLSLRLQVLLGQMPVTEGEIQRHIRRVGDVPQAPEASSQDQPMETTPVDCQNGAASPVPATTVEEVLFLPPQSSVPTICTDSEHPTQEDTGSEQWAASVPPEWVPVIRQDMQNQRKMKQQPPLSDAYLSGMPAKRRKTMQGEGPHLSLSEAVSRAMKATGAKPESSTDCVRRELDNSEAQGRYREQLCQDIQNILQDNESYSAQRFPNTQRAFRGDP

Target ORF information:

RefSeq Version	NM_001097409.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097409.2

ORF Insert Sequence:

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAGTAAGTGG GAATGATGGC TCCACTCTTG ATGTCCATAT AAATCTGGAT 
CAACAGCTCC CTGTCCAGAG TGAACCTCGT GTTCGTCTTG TTTTGGCTCA GCAAATACTG 
CAGGACATAC AGAGGATACT GGATCGTTTG GAGGGACAAC CAGTTAATGA GCAGACTGCA 
GAACCGATGG ACACAGCAGT GTCTGAAGGA GAGGCCAGCA GTAGAGAAAC CTTGCCTCAG 
ACTACACAGA ATACAGATGG GCAGTCGAAT ACAGCCCCTA CCAGTCATCC TTCACCATCA 
GAATATGTTG AGGTCCTGCA GTCTTTGTCA CGGGTTGAGG AACGTCTAGC CCCTTTCATG 
CAACGTTACC GGGAAATCCT TAGTAGTGCT ACCTCTGATG CCTATGAGAA TCAGGAAGAA 
AGGGAGCAGT CCCAGCGGAT AATTAATTTA GTTGGAGAAT CTCTTCGTCT CCTGGGAAAT 
GCCCTGGTTG CTGTATCCGA CTTGCGCTGC AATCTCTCTT CTGCTTCTCC CAGACACTTG 
CATGTTGTTC GCCCAATGTC CCATTACACT GGGCCCATGC TACTACAGCA AGCTGCTATA 
CCAATACAGA TTAATGTTGG TACTACAGTC ACTATGACTG GGAATGGAAC CCATGCTGGT 
CAGATGCCTT CAGATGGGAA TGCTGCTCAT ACTCCTACCA ATACTTCAGA ACCTCAGCGA 
TCTAACTCTG ACAACCAACC TCCATCCAGT GGAGAGAGAC CAGCTTCTGA GATCCCTCCC 
ACTTCAGTTC CACATCCCCA CCCTCGTGTT GTACGGATTA CGCACCAGAC TGTTGAGCCT 
GTCATGATGA TGCATATGAA CATTCAGGAT TCTGGACCTG GTGGCCCTAC AAACGTTCCC 
CCACCAACTG CGGGGCATGG GGGAAGTGCA CACATTCATA TGCCAGGATT ACCCCCTGAG 
TTCATGCAGG CAATTTCTCA CCAGATTACA CAGCAAGCTG TGGCTGCAGC TTCAGGGCAG 
CAAATTCCTG GCTTCCAAGC TCCACCACGC TTTGTTTTTA CTAGGCCTGC TGCCCCTTCA 
TTTCCACCCC AGCCTGGGGT AGCAACTACC CCACCAGGAC CTGGAGGGGC AACTACTGCT 
GTACCTGGGG CAACGGTGGG TCCAGCAGGA AATGCCTCCT TGGCTCAGAT GATCAGCGGT 
CTTGTTGGAC AGCTTCTGAT GCACCCAGTT ATTGTTGCTC AGGGTGGCAG CAACACCCCC 
TCTTCTACGT CAACCCCAAC TTCTACATCC TCCTCCAGCT CCTCTTCATC CTCCACTGTC 
ACTACCTCTA CAACAACCAC CTCTTCCACA TCCTTCCCCA CTGTTTCATC AGGTCCCTCT 
CCCCAGCCCC CACCTGGCAC TGATCAACAC CTCTCTCAGC TCCTGGGCTC TTTACTGGGA 
ACTGCCGGCT CAGGGATGTC AAACTTCGCT ATGGGTTCGC CGAGTATTAC TGTTACCGTG 
CCTGGAATGC CGGCCTTTTT ACAGGGTGTC ACAGATATAT TACAGGCTAC ACAGACTGTT 
CCTGTTTCTA CTTCTCCTCC CCAGTCTGCT TCTCAGGCTC CTCCTCCTTC ATCACCCTCA 
CCACCTCCTG CCCATTCCTC TCCCCCTCCA GCTGCCGCAC CAGAGTCTCT TCCTCCAGAG 
TTTTTCACTA GTGTTGTTCA GGGTGTCCTT TCATCCATGC TGGGGTCTTT GAGTGCAGCT 
GATCAGAGTG GAACAGAAAG CATTGCAGCT TTCATTCAGA GGCTCAGTGG CTCCCACAAC 
ATTTTCCAGC CTGATGCAGA GGGACCTGGT GGATTTTTTG GTGACTTACT CACCTTGATT 
TGTCACAATT TCTCCCTGGT CGATATGGTA ATGTTACTGC ATGGTCATTC CCAGCCTTTG 
CAGAATCTGC AGCCTCAACT GAGATCCTTC TTTCTTCAGG AGTATCTACA CCAGGCAGAT 
CCCACACCTA ATAACATCCA GATGGCATCA AGAAATCTTA CTAATGGCCT GGAAGAGTAC 
ATAAGAGAAA GCTTTGCCTC CGTTACTGTG CGTGATGATG TTGATATCAC AAGAACAAAC 
CTTGAATTTC TACAGGATCA GTTCAACAGG ATCACAACAC ACATCCTACA TTGTGCAGAT 
TCCACATTCG GACAGCGGCT TCTTGAGATG TGTAACCAGT CTCTCTTTGA ATGGCTTGCT 
CTCAACCTGT ATTGCCTCCG AGGGGACCAG AGTGCTCTCA CATCTGTCAT TAATGAGAGA 
ATTCGAAGAC TCTCTCTAGA TGTTTCACCT GTGCTTGTCT CGTGGGTGAC GTCTGTCCTG 
TCCCTCCGTC TTCAAGTGCT GTTGGGGCAG ATGCCTGTGA CTGAGGGCGA GATACAGAGA 
CATATACGGA GAGTGGGAGA TGTTCCGCAG GCTCCTGAAG CATCATCTCA GGATCAACCT 
ATGGAAACCA CACCTGTTGA TTGTCAGAAT GGTGCTGCCT CTCCAGTTCC TGCCACCACA 
GTGGAGGAGG TGCTTTTCCT CCCCCCTCAA AGTTCAGTAC CCACCATATG CACAGATTCT 
GAGCACCCAA CCCAAGAAGA TACCGGATCT GAGCAATGGG CAGCATCTGT GCCCCCAGAA 
TGGGTTCCTG TAATCAGACA AGATATGCAG AACCAGCGGA AGATGAAACA GCAGCCACCC 
TTGAGTGATG CCTACCTGAG TGGCATGCCT GCAAAGCGCC GCAAAACAAT GCAAGGTGAA 
GGGCCACACT TGTCCCTCTC TGAAGCTGTG AGCCGTGCAA TGAAAGCCAC AGGAGCCAAA 
CCAGAGAGCA GTACAGACTG TGTTAGGAGG GAGCTGGATA ACAGTGAAGC ACAAGGAAGG 
TACAGAGAAC AGTTGTGTCA GGATATCCAG AACATCCTCC AAGACAATGA GTCCTACAGT 
GCCCAGAGAT TCCCCAACAC ACAAAGGGCA TTCAGAGGGG ACCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bag6 ( NM_001244955.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001231884.1 Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant 2, mRNA.

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CloneID	OXa02171
Clone ID Related Accession (Same CDS sequence)	NM_001244955.1
Accession Version	NM_001244955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	large proline-rich protein bag6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT485713.1, CX916386.1, DT439387.1, EL825401.1 and CX913235.2. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: CT485713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAGTAAGTGG GAGTGAACCT CGTGTTCGTC TTGTTTTGGC TCAGCAAATA 
CTGCAGGACA TACAGAGGAT ACTGGATCGT TTGGAGGGAC AACCAGTTAA TGAGCAGACT 
GCAGAACCGA TGGACACAGC AGTGTCTGAA GGAGAGGCCA GCAGTAGAGA AACCTTGCCT 
CAGACTACAC AGAATACAGA TGGGCAGTCG AATACAGCCC CTACCAGTCA TCCTTCACCA 
TCAGAATATG TTGAGGTCCT GCAGTCTTTG TCACGGGTTG AGGAACGTCT AGCCCCTTTC 
ATGCAACGTT ACCGGGAAAT CCTTAGTAGT GCTACCTCTG ATGCCTATGA GAATCAGGAA 
GAAAGGGAGC AGTCCCAGCG GATAATTAAT TTAGTTGGAG AATCTCTTCG TCTCCTGGGA 
AATGCCCTGG TTGCTGTATC CGACTTGCGC TGCAATCTCT CTTCTGCTTC TCCCAGACAC 
TTGCATGTTG TTCGCCCAAT GTCCCATTAC ACTGGGCCCA TGCTACTACA GCAAGCTGCT 
ATACCAATAC AGATTAATGT TGGTACTACA GTCACTATGA CTGGGAATGG AACCCATGCT 
GGTCAGATGC CTTCAGATGG GAATGCTGCT CATACTCCTA CCAATACTTC AGAACCTCAG 
CGATCTAACT CTGACAACCA ACCTCCATCC AGTGGAGAGA GACCAGCTTC TGAGATCCCT 
CCCACTTCAG TTCCACATCC CCACCCTCGT GTTGTACGGA TTACGCACCA GACTGTTGAG 
CCTGTCATGA TGATGCATAT GAACATTCAG GATTCTGGAC CTGGTGGCCC TACAAACGTT 
CCCCCACCAA CTGCGGGGCA TGGGGGAAGT GCACACATTC ATATGCCAGG ATTACCCCCT 
GAGTTCATGC AGGCAATTTC TCACCAGATT ACACAGCAAG CTGTGGCTGC AGCTTCAGGG 
CAGCAAATTC CTGGCTTCCA AGCTCCACCA CGCTTTGTTT TTACTAGGCC TGCTGCCCCT 
TCATTTCCAC CCCAGCCTGG GGTAGCAACT ACCCCACCAG GACCTGGAGG GGCAACTACT 
GCTGTACCTG GGGAAACGGT GGGTCCAGCA GGAAATGCCT CCTTGGCTCA GATGATCAGC 
GGTCTTGTTG GACAGCTTCT GATGCACCCA GTTATTGTTG CTCAGGGTGG CAGCAACACC 
CCCTCTTCTA CGTCAACCCC AACTTCTACA TCCTCCTCCA GCTCCTCTTC ATCCTCCACT 
GTCACTACCT CTACAACAAC CACCTCTTCC ACATCCTTCC CCACTGTTTC ATCAGGTCCC 
TCTCCCCAGC CCCCACCTGG CACTGATCAA CACCTCTCTC AGCTCCTGGG CTCTTTACTG 
GGAACTGCCG GCTCAGGGAT GTCAAACTTC GCTATGGGTT CGCCGAGTAT TACTGTTACC 
GTGCCTGGAA TGCCGGCCTT TTTACAGGGT GTCACAGATA TATTACAGGC TACACAGACT 
GTTCCTGTTT CTACTTCTCC TCCCCAGTCT GCTTCTCAGG CTCCTCCTCC TTCATCACCC 
TCACCACCTC CTGCCCATTC CTCTCCCCCT CCAGCTGCCG CACCAGAGTC TCTTCCTCCA 
GAGTTTTTCA CTAGTGTTGT TCAGGGTGTC CTTTCATCCA TGCTGGGGTC TTTGAGTGCA 
GCTGATCAGA GTGGAACAGA AAGCATTGCA GCTTTCATTC AGAGGCTCAG TGGCTCCCAC 
AACATTTTCC AGCCTGATGC AGAGGGACCT GGTGGATTTT TTGGTGACTT ACTCACCTTG 
ATTTGTCACA ATTTCTCCCT GGTCGATATG GTAATGTTAC TGCATGGTCA TTCCCAGCCT 
TTGCAGAATC TGCAGCCTCA ACTGAGATCC TTCTTTCTTC AGGAGTATCT ACACCAGGCA 
GATCCCACAC CTAATAACAT CCAGATGGCA TCAAGAAATC TTACTAATGG CCTGGAAGAG 
TACATAAGAG AAAGCTTTGC CTCCGTTACT GTGCGTGATG ATGTTGATAT CACAAGAACA 
AACCTTGAAT TTCTACAGGA TCAGTTCAAC AGGATCACAA CACACATCCT ACATTGTGCA 
GATTCCACAT TCGGACAGCG GCTTCTTGAG ATGTGTAACC AGTCTCTCTT TGAATGGCTT 
GCTCTCAACC TGTATTGCCT CCGAGGGGAC CAGAGTGCTC TCACATCTGT CATTAATGAG 
AGAATTCGAA GACTCTCTCT AGATGTTTCA CCTGTGCTTG TCTCGTGGGT GACGTCTGTC 
CTGTCCCTCC GTCTTCAAGT GCTGTTGGGG CAGATGCCTG TGACTGAGGG CGAGATACAG 
AGACATATAC GGAGAGTGGG AGATGTTCCG CAGGCTCCTG AAGCATCATC TCAGGATCAA 
CCTATGGAAA CCACACCTGT TGATTGTCAG CAGAATGGTG CTGCCTCTCC AGTTCCTGCC 
ACCACAGTGG AGGAGGTGCT TTTCCTCCCC CCTCAAAGTT CAGTACCCAC CATATGCACA 
GATTCTGAGC ACCCAACCCA AGAAGATACC GGATCTGAGC AATGGGCAGC ATCTGTGCCC 
CCAGAATGGG TTCCTGTAAT CAGACAAGAT ATGCAGAACC AGCGGAAGAT GAAACAGCAG 
CCACCCTTGA GTGATGCCTA CCTGAGTGGC ATGCCTGCAA AGCGCCGCAA AACAATGCAA 
GGTGAAGGGC CACACTTGTC CCTCTCTGAA GCTGTGAGCC GTGCAATGAA AGCCACAGGA 
GCCAAACCAG AGAGCAGTAC AGACTGTGTT AGGAGGGAGC TGGATAACAG TGAAGCACAA 
GGAAGGTACA GAGAACAGTT GTGTCAGGAT ATCCAGAACA TCCTCCAAGA CAATGAGTCC 
TACAGTGCCC AGAGATTCCC CAACACACAA AGGGCATTCA GAGGGGACCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231884.1
CDS	107..3460
Translation	MAANEKMEVTVKTLDSQTRTFTVDAEITVKEFKTHISSDVGISPEKQRLIYQGRVLQEDKKLKEYNVDGKVIHLVERAPPQTQPPTGGPSTSSSTSPSSSNAANVPGAGAPERNGNSYVMVGTFNLPHVMSGLGEASRGPRVSTVSGSEPRVRLVLAQQILQDIQRILDRLEGQPVNEQTAEPMDTAVSEGEASSRETLPQTTQNTDGQSNTAPTSHPSPSEYVEVLQSLSRVEERLAPFMQRYREILSSATSDAYENQEEREQSQRIINLVGESLRLLGNALVAVSDLRCNLSSASPRHLHVVRPMSHYTGPMLLQQAAIPIQINVGTTVTMTGNGTHAGQMPSDGNAAHTPTNTSEPQRSNSDNQPPSSGERPASEIPPTSVPHPHPRVVRITHQTVEPVMMMHMNIQDSGPGGPTNVPPPTAGHGGSAHIHMPGLPPEFMQAISHQITQQAVAAASGQQIPGFQAPPRFVFTRPAAPSFPPQPGVATTPPGPGGATTAVPGETVGPAGNASLAQMISGLVGQLLMHPVIVAQGGSNTPSSTSTPTSTSSSSSSSSSTVTTSTTTTSSTSFPTVSSGPSPQPPPGTDQHLSQLLGSLLGTAGSGMSNFAMGSPSITVTVPGMPAFLQGVTDILQATQTVPVSTSPPQSASQAPPPSSPSPPPAHSSPPPAAAPESLPPEFFTSVVQGVLSSMLGSLSAADQSGTESIAAFIQRLSGSHNIFQPDAEGPGGFFGDLLTLICHNFSLVDMVMLLHGHSQPLQNLQPQLRSFFLQEYLHQADPTPNNIQMASRNLTNGLEEYIRESFASVTVRDDVDITRTNLEFLQDQFNRITTHILHCADSTFGQRLLEMCNQSLFEWLALNLYCLRGDQSALTSVINERIRRLSLDVSPVLVSWVTSVLSLRLQVLLGQMPVTEGEIQRHIRRVGDVPQAPEASSQDQPMETTPVDCQQNGAASPVPATTVEEVLFLPPQSSVPTICTDSEHPTQEDTGSEQWAASVPPEWVPVIRQDMQNQRKMKQQPPLSDAYLSGMPAKRRKTMQGEGPHLSLSEAVSRAMKATGAKPESSTDCVRRELDNSEAQGRYREQLCQDIQNILQDNESYSAQRFPNTQRAFRGDP

Target ORF information:

RefSeq Version	NM_001244955.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BAG cochaperone 6 (bag6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244955.1

ORF Insert Sequence:

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAGTAAGTGG GAGTGAACCT CGTGTTCGTC TTGTTTTGGC TCAGCAAATA 
CTGCAGGACA TACAGAGGAT ACTGGATCGT TTGGAGGGAC AACCAGTTAA TGAGCAGACT 
GCAGAACCGA TGGACACAGC AGTGTCTGAA GGAGAGGCCA GCAGTAGAGA AACCTTGCCT 
CAGACTACAC AGAATACAGA TGGGCAGTCG AATACAGCCC CTACCAGTCA TCCTTCACCA 
TCAGAATATG TTGAGGTCCT GCAGTCTTTG TCACGGGTTG AGGAACGTCT AGCCCCTTTC 
ATGCAACGTT ACCGGGAAAT CCTTAGTAGT GCTACCTCTG ATGCCTATGA GAATCAGGAA 
GAAAGGGAGC AGTCCCAGCG GATAATTAAT TTAGTTGGAG AATCTCTTCG TCTCCTGGGA 
AATGCCCTGG TTGCTGTATC CGACTTGCGC TGCAATCTCT CTTCTGCTTC TCCCAGACAC 
TTGCATGTTG TTCGCCCAAT GTCCCATTAC ACTGGGCCCA TGCTACTACA GCAAGCTGCT 
ATACCAATAC AGATTAATGT TGGTACTACA GTCACTATGA CTGGGAATGG AACCCATGCT 
GGTCAGATGC CTTCAGATGG GAATGCTGCT CATACTCCTA CCAATACTTC AGAACCTCAG 
CGATCTAACT CTGACAACCA ACCTCCATCC AGTGGAGAGA GACCAGCTTC TGAGATCCCT 
CCCACTTCAG TTCCACATCC CCACCCTCGT GTTGTACGGA TTACGCACCA GACTGTTGAG 
CCTGTCATGA TGATGCATAT GAACATTCAG GATTCTGGAC CTGGTGGCCC TACAAACGTT 
CCCCCACCAA CTGCGGGGCA TGGGGGAAGT GCACACATTC ATATGCCAGG ATTACCCCCT 
GAGTTCATGC AGGCAATTTC TCACCAGATT ACACAGCAAG CTGTGGCTGC AGCTTCAGGG 
CAGCAAATTC CTGGCTTCCA AGCTCCACCA CGCTTTGTTT TTACTAGGCC TGCTGCCCCT 
TCATTTCCAC CCCAGCCTGG GGTAGCAACT ACCCCACCAG GACCTGGAGG GGCAACTACT 
GCTGTACCTG GGGAAACGGT GGGTCCAGCA GGAAATGCCT CCTTGGCTCA GATGATCAGC 
GGTCTTGTTG GACAGCTTCT GATGCACCCA GTTATTGTTG CTCAGGGTGG CAGCAACACC 
CCCTCTTCTA CGTCAACCCC AACTTCTACA TCCTCCTCCA GCTCCTCTTC ATCCTCCACT 
GTCACTACCT CTACAACAAC CACCTCTTCC ACATCCTTCC CCACTGTTTC ATCAGGTCCC 
TCTCCCCAGC CCCCACCTGG CACTGATCAA CACCTCTCTC AGCTCCTGGG CTCTTTACTG 
GGAACTGCCG GCTCAGGGAT GTCAAACTTC GCTATGGGTT CGCCGAGTAT TACTGTTACC 
GTGCCTGGAA TGCCGGCCTT TTTACAGGGT GTCACAGATA TATTACAGGC TACACAGACT 
GTTCCTGTTT CTACTTCTCC TCCCCAGTCT GCTTCTCAGG CTCCTCCTCC TTCATCACCC 
TCACCACCTC CTGCCCATTC CTCTCCCCCT CCAGCTGCCG CACCAGAGTC TCTTCCTCCA 
GAGTTTTTCA CTAGTGTTGT TCAGGGTGTC CTTTCATCCA TGCTGGGGTC TTTGAGTGCA 
GCTGATCAGA GTGGAACAGA AAGCATTGCA GCTTTCATTC AGAGGCTCAG TGGCTCCCAC 
AACATTTTCC AGCCTGATGC AGAGGGACCT GGTGGATTTT TTGGTGACTT ACTCACCTTG 
ATTTGTCACA ATTTCTCCCT GGTCGATATG GTAATGTTAC TGCATGGTCA TTCCCAGCCT 
TTGCAGAATC TGCAGCCTCA ACTGAGATCC TTCTTTCTTC AGGAGTATCT ACACCAGGCA 
GATCCCACAC CTAATAACAT CCAGATGGCA TCAAGAAATC TTACTAATGG CCTGGAAGAG 
TACATAAGAG AAAGCTTTGC CTCCGTTACT GTGCGTGATG ATGTTGATAT CACAAGAACA 
AACCTTGAAT TTCTACAGGA TCAGTTCAAC AGGATCACAA CACACATCCT ACATTGTGCA 
GATTCCACAT TCGGACAGCG GCTTCTTGAG ATGTGTAACC AGTCTCTCTT TGAATGGCTT 
GCTCTCAACC TGTATTGCCT CCGAGGGGAC CAGAGTGCTC TCACATCTGT CATTAATGAG 
AGAATTCGAA GACTCTCTCT AGATGTTTCA CCTGTGCTTG TCTCGTGGGT GACGTCTGTC 
CTGTCCCTCC GTCTTCAAGT GCTGTTGGGG CAGATGCCTG TGACTGAGGG CGAGATACAG 
AGACATATAC GGAGAGTGGG AGATGTTCCG CAGGCTCCTG AAGCATCATC TCAGGATCAA 
CCTATGGAAA CCACACCTGT TGATTGTCAG CAGAATGGTG CTGCCTCTCC AGTTCCTGCC 
ACCACAGTGG AGGAGGTGCT TTTCCTCCCC CCTCAAAGTT CAGTACCCAC CATATGCACA 
GATTCTGAGC ACCCAACCCA AGAAGATACC GGATCTGAGC AATGGGCAGC ATCTGTGCCC 
CCAGAATGGG TTCCTGTAAT CAGACAAGAT ATGCAGAACC AGCGGAAGAT GAAACAGCAG 
CCACCCTTGA GTGATGCCTA CCTGAGTGGC ATGCCTGCAA AGCGCCGCAA AACAATGCAA 
GGTGAAGGGC CACACTTGTC CCTCTCTGAA GCTGTGAGCC GTGCAATGAA AGCCACAGGA 
GCCAAACCAG AGAGCAGTAC AGACTGTGTT AGGAGGGAGC TGGATAACAG TGAAGCACAA 
GGAAGGTACA GAGAACAGTT GTGTCAGGAT ATCCAGAACA TCCTCCAAGA CAATGAGTCC 
TACAGTGCCC AGAGATTCCC CAACACACAA AGGGCATTCA GAGGGGACCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bag6 ( XM_012967838.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823292.1 Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X1, mRNA.

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CloneID	OXa30198
Clone ID Related Accession (Same CDS sequence)	XM_012967838.2
Accession Version	XM_012967838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	large proline-rich protein bag6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAGTAAGTGG GAATGATGGC TCCACTCTTG ATGTCCATAT AAATCTGGAT 
CAACAGCTCC CTGTCCAGAG TGAACCTCGT GTTCGTCTTG TTTTGGCTCA GCAAATACTG 
CAGGACATAC AGAGGATACT GGATCGTTTG GAGGGACAAC CAGTTAATGA GCAGACTGCA 
GAACCGATGG ACACAGCAGT GTCTGAAGGA GAGGCCAGCA GTAGAGAAAC CTTGCCTCAG 
ACTACACAGA ATACAGATGG GCAGTCGAAT ACAGCCCCTA CCAGTCATCC TTCACCATCA 
GAATATGTTG AGGTCCTGCA GTCTTTGTCA CGGGTTGAGG AACGTCTAGC CCCTTTCATG 
CAACGTTACC GGGAAATCCT TAGTAGTGCT ACCTCTGATG CCTATGAGAA TCAGGAAGAA 
AGGGAGCAGT CCCAGCGGAT AATTAATTTA GTTGGAGAAT CTCTTCGTCT CCTGGGAAAT 
GCCCTGGTTG CTGTATCCGA CTTGCGCTGC AATCTCTCTT CTGCTTCTCC CAGACACTTG 
CATGTTGTTC GCCCAATGTC CCATTACACT GGGCCCATGC TACTACAGCA AGCTGCTATA 
CCAATACAGA TTAATGTTGG TACTACAGTC ACTATGACTG GGAATGGAAC CCATGCTGGT 
CAGATGCCTT CAGATGGGAA TGCTGCTCAT ACTCCTACCA ATACTTCAGA ACCTCAGCGA 
TCTAACTCTG ACAACCAACC TCCATCCAGT GGAGAGAGAC CAGCTTCTGA GATCCCTCCC 
ACTTCAGTTC CACATCCCCA CCCTCGTGTT GTACGGATTA CGCACCAGAC TGTTGAGCCT 
GTCATGATGA TGCATATGAA CATTCAGGAT TCTGGACCTG GTGGCCCTAC AAACGTTCCC 
CCACCAACTG CGGGGCATGG GGGAAGTGCA CACATTCATA TGCCAGGATT ACCCCCTGAG 
TTCATGCAGG CAATTTCTCA CCAGATTACA CAGCAAGCTG TGGCTGCAGC TTCAGGGCAG 
CAAATTCCTG GCTTCCAAGC TCCACCACGC TTTGTTTTTA CTAGGCCTGC TGCCCCTTCA 
TTTCCACCCC AGCCTGGGGT AGCAACTACC CCACCAGGAC CTGGAGGGGC AACTACTGCT 
GTACCTGGGG CAACGGTGGG TCCAGCAGGA AATGCCTCCT TGGCTCAGAT GATCAGCGGT 
CTTGTTGGAC AGCTTCTGAT GCACCCAGTT ATTGTTGCTC AGGGTGGCAG CAACACCCCC 
TCTTCTACGT CAACCCCAAC TTCTACATCC TCCTCCAGCT CCTCTTCATC CTCCACTGTC 
ACTACCTCTA CAACAACCAC CTCTTCCACA TCCTTCCCCA CTGTTTCATC AGGTCCCTCT 
CCCCAGCCCC CACCTGGCAC TGATCAACAC CTCTCTCAGC TCCTGGGCTC TTTACTGGGA 
ACTGCCGGCT CAGGGATGTC AAACTTCGCT ATGGGTTCGC CGAGTATTAC TGTTACCGTG 
CCTGGAATGC CGGCCTTTTT ACAGGGTGTC ACAGATATAT TACAGGCTAC ACAGACTGTT 
CCTGTTTCTA CTTCTCCTCC CCAGTCTGCT TCTCAGGCTC CTCCTCCTTC ATCACCCTCA 
CCACCTCCTG CCCATTCCTC TCCCCCTCCA GCTGCCGCAC CAGAGTCTCT TCCTCCAGAG 
TTTTTCACTA GTGTTGTTCA GGGTGTCCTT TCATCCATGC TGGGGTCTTT GAGTGCAGCT 
GATCAGAGTG GAACAGAAAG CATTGCAGCT TTCATTCAGA GGCTCAGTGG CTCCCACAAC 
ATTTTCCAGC CTGATGCAGA GGGACCTGGT GGATTTTTTG GTGACTTACT CACCTTGATT 
TGTCACAATT TCTCCCTGGT CGATATGGTA ATGTTACTGC ATGGTCATTC CCAGCCTTTG 
CAGAATCTGC AGCCTCAACT GAGATCCTTC TTTCTTCAGG AGTATCTACA CCAGGCAGAT 
CCCACACCTA ATAACATCCA GATGGCATCA AGAAATCTTA CTAATGGCCT GGAAGAGTAC 
ATAAGAGAAA GCTTTGCCTC CGTTACTGTG CGTGATGATG TTGATATCAC AAGAACAAAC 
CTTGAATTTC TACAGGATCA GTTCAACAGG ATCACAACAC ACATCCTACA TTGTGCAGAT 
TCCACATTCG GACAGCGGCT TCTTGAGATG TGTAACCAGT CTCTCTTTGA ATGGCTTGCT 
CTCAACCTGT ATTGCCTCCG AGGGGACCAG AGTGCTCTCA CATCTGTCAT TAATGAGAGA 
ATTCGAAGAC TCTCTCTAGA TGTTTCACCT GTGCTTGTCT CGTGGGTGAC GTCTGTCCTG 
TCCCTCCGTC TTCAAGTGCT GTTGGGGCAG ATGCCTGTGA CTGAGGGCGA GATACAGAGA 
CATATACGGA GAGTGGGAGA TGTTCCGCAG GCTCCTGAAG CATCATCTCA GGATCAACCT 
ATGGAAACCA CACCTGTTGA TTGTCAGCAG AATGGTGCTG CCTCTCCAGT TCCTGCCACC 
ACAGTGGAGG AGGTGCTTTT CCTCCCCCCT CAAAGTTCAG TACCCACCAT ATGCACAGAT 
TCTGAGCACC CAACCCAAGA AGATACCGGA TCTGAGCAAT GGGCAGCATC TGTGCCCCCA 
GAATGGGTTC CTGTAATCAG ACAAGATATG CAGAACCAGC GGAAGATGAA ACAGCAGCCA 
CCCTTGAGTG ATGCCTACCT GAGTGGCATG CCTGCAAAGC GCCGCAAAAC AATGCAAGGT 
GAAGGGCCAC ACTTGTCCCT CTCTGAAGCT GTGAGCCGTG CAATGAAAGC CACAGGAGCC 
AAACCAGAGA GCAGTACAGA CTGTGTTAGG AGGGAGCTGG ATAACAGTGA AGCACAAGGA 
AGGTACAGAG AACAGTTGTG TCAGGATATC CAGAACATCC TCCAAGACAA TGAGTCCTAC 
AGTGCCCAGA GATTCCCCAA CACACAAAGG GCATTCAGAG GGGACCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823292.1
CDS	191..3601
Translation	MAANEKMEVTVKTLDSQTRTFTVDAEITVKEFKTHISSDVGISPEKQRLIYQGRVLQEDKKLKEYNVDGKVIHLVERAPPQTQPPTGGPSTSSSTSPSSSNAANVPGAGAPERNGNSYVMVGTFNLPHVMSGLGEASRGPRVSTVSGNDGSTLDVHINLDQQLPVQSEPRVRLVLAQQILQDIQRILDRLEGQPVNEQTAEPMDTAVSEGEASSRETLPQTTQNTDGQSNTAPTSHPSPSEYVEVLQSLSRVEERLAPFMQRYREILSSATSDAYENQEEREQSQRIINLVGESLRLLGNALVAVSDLRCNLSSASPRHLHVVRPMSHYTGPMLLQQAAIPIQINVGTTVTMTGNGTHAGQMPSDGNAAHTPTNTSEPQRSNSDNQPPSSGERPASEIPPTSVPHPHPRVVRITHQTVEPVMMMHMNIQDSGPGGPTNVPPPTAGHGGSAHIHMPGLPPEFMQAISHQITQQAVAAASGQQIPGFQAPPRFVFTRPAAPSFPPQPGVATTPPGPGGATTAVPGATVGPAGNASLAQMISGLVGQLLMHPVIVAQGGSNTPSSTSTPTSTSSSSSSSSSTVTTSTTTTSSTSFPTVSSGPSPQPPPGTDQHLSQLLGSLLGTAGSGMSNFAMGSPSITVTVPGMPAFLQGVTDILQATQTVPVSTSPPQSASQAPPPSSPSPPPAHSSPPPAAAPESLPPEFFTSVVQGVLSSMLGSLSAADQSGTESIAAFIQRLSGSHNIFQPDAEGPGGFFGDLLTLICHNFSLVDMVMLLHGHSQPLQNLQPQLRSFFLQEYLHQADPTPNNIQMASRNLTNGLEEYIRESFASVTVRDDVDITRTNLEFLQDQFNRITTHILHCADSTFGQRLLEMCNQSLFEWLALNLYCLRGDQSALTSVINERIRRLSLDVSPVLVSWVTSVLSLRLQVLLGQMPVTEGEIQRHIRRVGDVPQAPEASSQDQPMETTPVDCQQNGAASPVPATTVEEVLFLPPQSSVPTICTDSEHPTQEDTGSEQWAASVPPEWVPVIRQDMQNQRKMKQQPPLSDAYLSGMPAKRRKTMQGEGPHLSLSEAVSRAMKATGAKPESSTDCVRRELDNSEAQGRYREQLCQDIQNILQDNESYSAQRFPNTQRAFRGDP

Target ORF information:

RefSeq Version	XM_012967838.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967838.2

ORF Insert Sequence:

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAGTAAGTGG GAATGATGGC TCCACTCTTG ATGTCCATAT AAATCTGGAT 
CAACAGCTCC CTGTCCAGAG TGAACCTCGT GTTCGTCTTG TTTTGGCTCA GCAAATACTG 
CAGGACATAC AGAGGATACT GGATCGTTTG GAGGGACAAC CAGTTAATGA GCAGACTGCA 
GAACCGATGG ACACAGCAGT GTCTGAAGGA GAGGCCAGCA GTAGAGAAAC CTTGCCTCAG 
ACTACACAGA ATACAGATGG GCAGTCGAAT ACAGCCCCTA CCAGTCATCC TTCACCATCA 
GAATATGTTG AGGTCCTGCA GTCTTTGTCA CGGGTTGAGG AACGTCTAGC CCCTTTCATG 
CAACGTTACC GGGAAATCCT TAGTAGTGCT ACCTCTGATG CCTATGAGAA TCAGGAAGAA 
AGGGAGCAGT CCCAGCGGAT AATTAATTTA GTTGGAGAAT CTCTTCGTCT CCTGGGAAAT 
GCCCTGGTTG CTGTATCCGA CTTGCGCTGC AATCTCTCTT CTGCTTCTCC CAGACACTTG 
CATGTTGTTC GCCCAATGTC CCATTACACT GGGCCCATGC TACTACAGCA AGCTGCTATA 
CCAATACAGA TTAATGTTGG TACTACAGTC ACTATGACTG GGAATGGAAC CCATGCTGGT 
CAGATGCCTT CAGATGGGAA TGCTGCTCAT ACTCCTACCA ATACTTCAGA ACCTCAGCGA 
TCTAACTCTG ACAACCAACC TCCATCCAGT GGAGAGAGAC CAGCTTCTGA GATCCCTCCC 
ACTTCAGTTC CACATCCCCA CCCTCGTGTT GTACGGATTA CGCACCAGAC TGTTGAGCCT 
GTCATGATGA TGCATATGAA CATTCAGGAT TCTGGACCTG GTGGCCCTAC AAACGTTCCC 
CCACCAACTG CGGGGCATGG GGGAAGTGCA CACATTCATA TGCCAGGATT ACCCCCTGAG 
TTCATGCAGG CAATTTCTCA CCAGATTACA CAGCAAGCTG TGGCTGCAGC TTCAGGGCAG 
CAAATTCCTG GCTTCCAAGC TCCACCACGC TTTGTTTTTA CTAGGCCTGC TGCCCCTTCA 
TTTCCACCCC AGCCTGGGGT AGCAACTACC CCACCAGGAC CTGGAGGGGC AACTACTGCT 
GTACCTGGGG CAACGGTGGG TCCAGCAGGA AATGCCTCCT TGGCTCAGAT GATCAGCGGT 
CTTGTTGGAC AGCTTCTGAT GCACCCAGTT ATTGTTGCTC AGGGTGGCAG CAACACCCCC 
TCTTCTACGT CAACCCCAAC TTCTACATCC TCCTCCAGCT CCTCTTCATC CTCCACTGTC 
ACTACCTCTA CAACAACCAC CTCTTCCACA TCCTTCCCCA CTGTTTCATC AGGTCCCTCT 
CCCCAGCCCC CACCTGGCAC TGATCAACAC CTCTCTCAGC TCCTGGGCTC TTTACTGGGA 
ACTGCCGGCT CAGGGATGTC AAACTTCGCT ATGGGTTCGC CGAGTATTAC TGTTACCGTG 
CCTGGAATGC CGGCCTTTTT ACAGGGTGTC ACAGATATAT TACAGGCTAC ACAGACTGTT 
CCTGTTTCTA CTTCTCCTCC CCAGTCTGCT TCTCAGGCTC CTCCTCCTTC ATCACCCTCA 
CCACCTCCTG CCCATTCCTC TCCCCCTCCA GCTGCCGCAC CAGAGTCTCT TCCTCCAGAG 
TTTTTCACTA GTGTTGTTCA GGGTGTCCTT TCATCCATGC TGGGGTCTTT GAGTGCAGCT 
GATCAGAGTG GAACAGAAAG CATTGCAGCT TTCATTCAGA GGCTCAGTGG CTCCCACAAC 
ATTTTCCAGC CTGATGCAGA GGGACCTGGT GGATTTTTTG GTGACTTACT CACCTTGATT 
TGTCACAATT TCTCCCTGGT CGATATGGTA ATGTTACTGC ATGGTCATTC CCAGCCTTTG 
CAGAATCTGC AGCCTCAACT GAGATCCTTC TTTCTTCAGG AGTATCTACA CCAGGCAGAT 
CCCACACCTA ATAACATCCA GATGGCATCA AGAAATCTTA CTAATGGCCT GGAAGAGTAC 
ATAAGAGAAA GCTTTGCCTC CGTTACTGTG CGTGATGATG TTGATATCAC AAGAACAAAC 
CTTGAATTTC TACAGGATCA GTTCAACAGG ATCACAACAC ACATCCTACA TTGTGCAGAT 
TCCACATTCG GACAGCGGCT TCTTGAGATG TGTAACCAGT CTCTCTTTGA ATGGCTTGCT 
CTCAACCTGT ATTGCCTCCG AGGGGACCAG AGTGCTCTCA CATCTGTCAT TAATGAGAGA 
ATTCGAAGAC TCTCTCTAGA TGTTTCACCT GTGCTTGTCT CGTGGGTGAC GTCTGTCCTG 
TCCCTCCGTC TTCAAGTGCT GTTGGGGCAG ATGCCTGTGA CTGAGGGCGA GATACAGAGA 
CATATACGGA GAGTGGGAGA TGTTCCGCAG GCTCCTGAAG CATCATCTCA GGATCAACCT 
ATGGAAACCA CACCTGTTGA TTGTCAGCAG AATGGTGCTG CCTCTCCAGT TCCTGCCACC 
ACAGTGGAGG AGGTGCTTTT CCTCCCCCCT CAAAGTTCAG TACCCACCAT ATGCACAGAT 
TCTGAGCACC CAACCCAAGA AGATACCGGA TCTGAGCAAT GGGCAGCATC TGTGCCCCCA 
GAATGGGTTC CTGTAATCAG ACAAGATATG CAGAACCAGC GGAAGATGAA ACAGCAGCCA 
CCCTTGAGTG ATGCCTACCT GAGTGGCATG CCTGCAAAGC GCCGCAAAAC AATGCAAGGT 
GAAGGGCCAC ACTTGTCCCT CTCTGAAGCT GTGAGCCGTG CAATGAAAGC CACAGGAGCC 
AAACCAGAGA GCAGTACAGA CTGTGTTAGG AGGGAGCTGG ATAACAGTGA AGCACAAGGA 
AGGTACAGAG AACAGTTGTG TCAGGATATC CAGAACATCC TCCAAGACAA TGAGTCCTAC 
AGTGCCCAGA GATTCCCCAA CACACAAAGG GCATTCAGAG GGGACCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bag6 ( XM_012967839.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823293.1 Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X3, mRNA.

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CloneID	OXa30199
Clone ID Related Accession (Same CDS sequence)	XM_012967839.2
Accession Version	XM_012967839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	large proline-rich protein bag6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAAGTGAACC TCGTGTTCGT CTTGTTTTGG CTCAGCAAAT ACTGCAGGAC 
ATACAGAGGA TACTGGATCG TTTGGAGGGA CAACCAGTTA ATGAGCAGAC TGCAGAACCG 
ATGGACACAG CAGTGTCTGA AGGAGAGGCC AGCAGTAGAG AAACCTTGCC TCAGACTACA 
CAGAATACAG ATGGGCAGTC GAATACAGCC CCTACCAGTC ATCCTTCACC ATCAGAATAT 
GTTGAGGTCC TGCAGTCTTT GTCACGGGTT GAGGAACGTC TAGCCCCTTT CATGCAACGT 
TACCGGGAAA TCCTTAGTAG TGCTACCTCT GATGCCTATG AGAATCAGGA AGAAAGGGAG 
CAGTCCCAGC GGATAATTAA TTTAGTTGGA GAATCTCTTC GTCTCCTGGG AAATGCCCTG 
GTTGCTGTAT CCGACTTGCG CTGCAATCTC TCTTCTGCTT CTCCCAGACA CTTGCATGTT 
GTTCGCCCAA TGTCCCATTA CACTGGGCCC ATGCTACTAC AGCAAGCTGC TATACCAATA 
CAGATTAATG TTGGTACTAC AGTCACTATG ACTGGGAATG GAACCCATGC TGGTCAGATG 
CCTTCAGATG GGAATGCTGC TCATACTCCT ACCAATACTT CAGAACCTCA GCGATCTAAC 
TCTGACAACC AACCTCCATC CAGTGGAGAG AGACCAGCTT CTGAGATCCC TCCCACTTCA 
GTTCCACATC CCCACCCTCG TGTTGTACGG ATTACGCACC AGACTGTTGA GCCTGTCATG 
ATGATGCATA TGAACATTCA GGATTCTGGA CCTGGTGGCC CTACAAACGT TCCCCCACCA 
ACTGCGGGGC ATGGGGGAAG TGCACACATT CATATGCCAG GATTACCCCC TGAGTTCATG 
CAGGCAATTT CTCACCAGAT TACACAGCAA GCTGTGGCTG CAGCTTCAGG GCAGCAAATT 
CCTGGCTTCC AAGCTCCACC ACGCTTTGTT TTTACTAGGC CTGCTGCCCC TTCATTTCCA 
CCCCAGCCTG GGGTAGCAAC TACCCCACCA GGACCTGGAG GGGCAACTAC TGCTGTACCT 
GGGGCAACGG TGGGTCCAGC AGGAAATGCC TCCTTGGCTC AGATGATCAG CGGTCTTGTT 
GGACAGCTTC TGATGCACCC AGTTATTGTT GCTCAGGGTG GCAGCAACAC CCCCTCTTCT 
ACGTCAACCC CAACTTCTAC ATCCTCCTCC AGCTCCTCTT CATCCTCCAC TGTCACTACC 
TCTACAACAA CCACCTCTTC CACATCCTTC CCCACTGTTT CATCAGGTCC CTCTCCCCAG 
CCCCCACCTG GCACTGATCA ACACCTCTCT CAGCTCCTGG GCTCTTTACT GGGAACTGCC 
GGCTCAGGGA TGTCAAACTT CGCTATGGGT TCGCCGAGTA TTACTGTTAC CGTGCCTGGA 
ATGCCGGCCT TTTTACAGGG TGTCACAGAT ATATTACAGG CTACACAGAC TGTTCCTGTT 
TCTACTTCTC CTCCCCAGTC TGCTTCTCAG GCTCCTCCTC CTTCATCACC CTCACCACCT 
CCTGCCCATT CCTCTCCCCC TCCAGCTGCC GCACCAGAGT CTCTTCCTCC AGAGTTTTTC 
ACTAGTGTTG TTCAGGGTGT CCTTTCATCC ATGCTGGGGT CTTTGAGTGC AGCTGATCAG 
AGTGGAACAG AAAGCATTGC AGCTTTCATT CAGAGGCTCA GTGGCTCCCA CAACATTTTC 
CAGCCTGATG CAGAGGGACC TGGTGGATTT TTTGGTGACT TACTCACCTT GATTTGTCAC 
AATTTCTCCC TGGTCGATAT GGTAATGTTA CTGCATGGTC ATTCCCAGCC TTTGCAGAAT 
CTGCAGCCTC AACTGAGATC CTTCTTTCTT CAGGAGTATC TACACCAGGC AGATCCCACA 
CCTAATAACA TCCAGATGGC ATCAAGAAAT CTTACTAATG GCCTGGAAGA GTACATAAGA 
GAAAGCTTTG CCTCCGTTAC TGTGCGTGAT GATGTTGATA TCACAAGAAC AAACCTTGAA 
TTTCTACAGG ATCAGTTCAA CAGGATCACA ACACACATCC TACATTGTGC AGATTCCACA 
TTCGGACAGC GGCTTCTTGA GATGTGTAAC CAGTCTCTCT TTGAATGGCT TGCTCTCAAC 
CTGTATTGCC TCCGAGGGGA CCAGAGTGCT CTCACATCTG TCATTAATGA GAGAATTCGA 
AGACTCTCTC TAGATGTTTC ACCTGTGCTT GTCTCGTGGG TGACGTCTGT CCTGTCCCTC 
CGTCTTCAAG TGCTGTTGGG GCAGATGCCT GTGACTGAGG GCGAGATACA GAGACATATA 
CGGAGAGTGG GAGATGTTCC GCAGGCTCCT GAAGCATCAT CTCAGGATCA ACCTATGGAA 
ACCACACCTG TTGATTGTCA GCAGAATGGT GCTGCCTCTC CAGTTCCTGC CACCACAGTG 
GAGGAGGTGC TTTTCCTCCC CCCTCAAAGT TCAGTACCCA CCATATGCAC AGATTCTGAG 
CACCCAACCC AAGAAGATAC CGGATCTGAG CAATGGGCAG CATCTGTGCC CCCAGAATGG 
GTTCCTGTAA TCAGACAAGA TATGCAGAAC CAGCGGAAGA TGAAACAGCA GCCACCCTTG 
AGTGATGCCT ACCTGAGTGG CATGCCTGCA AAGCGCCGCA AAACAATGCA AGGTGAAGGG 
CCACACTTGT CCCTCTCTGA AGCTGTGAGC CGTGCAATGA AAGCCACAGG AGCCAAACCA 
GAGAGCAGTA CAGACTGTGT TAGGAGGGAG CTGGATAACA GTGAAGCACA AGGAAGGTAC 
AGAGAACAGT TGTGTCAGGA TATCCAGAAC ATCCTCCAAG ACAATGAGTC CTACAGTGCC 
CAGAGATTCC CCAACACACA AAGGGCATTC AGAGGGGACC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823293.1
CDS	190..3534
Translation	MAANEKMEVTVKTLDSQTRTFTVDAEITVKEFKTHISSDVGISPEKQRLIYQGRVLQEDKKLKEYNVDGKVIHLVERAPPQTQPPTGGPSTSSSTSPSSSNAANVPGAGAPERNGNSYVMVGTFNLPHVMSGLGEASRGPRVSTSEPRVRLVLAQQILQDIQRILDRLEGQPVNEQTAEPMDTAVSEGEASSRETLPQTTQNTDGQSNTAPTSHPSPSEYVEVLQSLSRVEERLAPFMQRYREILSSATSDAYENQEEREQSQRIINLVGESLRLLGNALVAVSDLRCNLSSASPRHLHVVRPMSHYTGPMLLQQAAIPIQINVGTTVTMTGNGTHAGQMPSDGNAAHTPTNTSEPQRSNSDNQPPSSGERPASEIPPTSVPHPHPRVVRITHQTVEPVMMMHMNIQDSGPGGPTNVPPPTAGHGGSAHIHMPGLPPEFMQAISHQITQQAVAAASGQQIPGFQAPPRFVFTRPAAPSFPPQPGVATTPPGPGGATTAVPGATVGPAGNASLAQMISGLVGQLLMHPVIVAQGGSNTPSSTSTPTSTSSSSSSSSSTVTTSTTTTSSTSFPTVSSGPSPQPPPGTDQHLSQLLGSLLGTAGSGMSNFAMGSPSITVTVPGMPAFLQGVTDILQATQTVPVSTSPPQSASQAPPPSSPSPPPAHSSPPPAAAPESLPPEFFTSVVQGVLSSMLGSLSAADQSGTESIAAFIQRLSGSHNIFQPDAEGPGGFFGDLLTLICHNFSLVDMVMLLHGHSQPLQNLQPQLRSFFLQEYLHQADPTPNNIQMASRNLTNGLEEYIRESFASVTVRDDVDITRTNLEFLQDQFNRITTHILHCADSTFGQRLLEMCNQSLFEWLALNLYCLRGDQSALTSVINERIRRLSLDVSPVLVSWVTSVLSLRLQVLLGQMPVTEGEIQRHIRRVGDVPQAPEASSQDQPMETTPVDCQQNGAASPVPATTVEEVLFLPPQSSVPTICTDSEHPTQEDTGSEQWAASVPPEWVPVIRQDMQNQRKMKQQPPLSDAYLSGMPAKRRKTMQGEGPHLSLSEAVSRAMKATGAKPESSTDCVRRELDNSEAQGRYREQLCQDIQNILQDNESYSAQRFPNTQRAFRGDP

Target ORF information:

RefSeq Version	XM_012967839.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BCL2 associated athanogene 6 (bag6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967839.2

ORF Insert Sequence:

ATGGCAGCTA ATGAGAAAAT GGAAGTGACT GTGAAAACAC TGGATTCACA AACAAGAACA 
TTCACAGTGG ATGCAGAGAT AACAGTAAAG GAATTCAAAA CACATATTTC ATCTGATGTT 
GGAATTTCAC CAGAGAAACA GCGGCTTATA TATCAGGGGC GAGTACTGCA AGAGGACAAA 
AAACTGAAAG AATACAATGT TGATGGGAAG GTTATTCACT TAGTTGAACG GGCTCCTCCT 
CAGACACAGC CTCCAACAGG TGGACCATCT ACATCTTCAA GTACTTCACC ATCCAGCAGT 
AATGCCGCTA ATGTACCTGG GGCTGGGGCT CCTGAAAGGA ATGGAAACAG CTATGTGATG 
GTTGGCACAT TCAATCTTCC GCATGTAATG TCTGGTCTTG GTGAGGCCAG CAGAGGCCCA 
CGTGTTTCTA CAAGTGAACC TCGTGTTCGT CTTGTTTTGG CTCAGCAAAT ACTGCAGGAC 
ATACAGAGGA TACTGGATCG TTTGGAGGGA CAACCAGTTA ATGAGCAGAC TGCAGAACCG 
ATGGACACAG CAGTGTCTGA AGGAGAGGCC AGCAGTAGAG AAACCTTGCC TCAGACTACA 
CAGAATACAG ATGGGCAGTC GAATACAGCC CCTACCAGTC ATCCTTCACC ATCAGAATAT 
GTTGAGGTCC TGCAGTCTTT GTCACGGGTT GAGGAACGTC TAGCCCCTTT CATGCAACGT 
TACCGGGAAA TCCTTAGTAG TGCTACCTCT GATGCCTATG AGAATCAGGA AGAAAGGGAG 
CAGTCCCAGC GGATAATTAA TTTAGTTGGA GAATCTCTTC GTCTCCTGGG AAATGCCCTG 
GTTGCTGTAT CCGACTTGCG CTGCAATCTC TCTTCTGCTT CTCCCAGACA CTTGCATGTT 
GTTCGCCCAA TGTCCCATTA CACTGGGCCC ATGCTACTAC AGCAAGCTGC TATACCAATA 
CAGATTAATG TTGGTACTAC AGTCACTATG ACTGGGAATG GAACCCATGC TGGTCAGATG 
CCTTCAGATG GGAATGCTGC TCATACTCCT ACCAATACTT CAGAACCTCA GCGATCTAAC 
TCTGACAACC AACCTCCATC CAGTGGAGAG AGACCAGCTT CTGAGATCCC TCCCACTTCA 
GTTCCACATC CCCACCCTCG TGTTGTACGG ATTACGCACC AGACTGTTGA GCCTGTCATG 
ATGATGCATA TGAACATTCA GGATTCTGGA CCTGGTGGCC CTACAAACGT TCCCCCACCA 
ACTGCGGGGC ATGGGGGAAG TGCACACATT CATATGCCAG GATTACCCCC TGAGTTCATG 
CAGGCAATTT CTCACCAGAT TACACAGCAA GCTGTGGCTG CAGCTTCAGG GCAGCAAATT 
CCTGGCTTCC AAGCTCCACC ACGCTTTGTT TTTACTAGGC CTGCTGCCCC TTCATTTCCA 
CCCCAGCCTG GGGTAGCAAC TACCCCACCA GGACCTGGAG GGGCAACTAC TGCTGTACCT 
GGGGCAACGG TGGGTCCAGC AGGAAATGCC TCCTTGGCTC AGATGATCAG CGGTCTTGTT 
GGACAGCTTC TGATGCACCC AGTTATTGTT GCTCAGGGTG GCAGCAACAC CCCCTCTTCT 
ACGTCAACCC CAACTTCTAC ATCCTCCTCC AGCTCCTCTT CATCCTCCAC TGTCACTACC 
TCTACAACAA CCACCTCTTC CACATCCTTC CCCACTGTTT CATCAGGTCC CTCTCCCCAG 
CCCCCACCTG GCACTGATCA ACACCTCTCT CAGCTCCTGG GCTCTTTACT GGGAACTGCC 
GGCTCAGGGA TGTCAAACTT CGCTATGGGT TCGCCGAGTA TTACTGTTAC CGTGCCTGGA 
ATGCCGGCCT TTTTACAGGG TGTCACAGAT ATATTACAGG CTACACAGAC TGTTCCTGTT 
TCTACTTCTC CTCCCCAGTC TGCTTCTCAG GCTCCTCCTC CTTCATCACC CTCACCACCT 
CCTGCCCATT CCTCTCCCCC TCCAGCTGCC GCACCAGAGT CTCTTCCTCC AGAGTTTTTC 
ACTAGTGTTG TTCAGGGTGT CCTTTCATCC ATGCTGGGGT CTTTGAGTGC AGCTGATCAG 
AGTGGAACAG AAAGCATTGC AGCTTTCATT CAGAGGCTCA GTGGCTCCCA CAACATTTTC 
CAGCCTGATG CAGAGGGACC TGGTGGATTT TTTGGTGACT TACTCACCTT GATTTGTCAC 
AATTTCTCCC TGGTCGATAT GGTAATGTTA CTGCATGGTC ATTCCCAGCC TTTGCAGAAT 
CTGCAGCCTC AACTGAGATC CTTCTTTCTT CAGGAGTATC TACACCAGGC AGATCCCACA 
CCTAATAACA TCCAGATGGC ATCAAGAAAT CTTACTAATG GCCTGGAAGA GTACATAAGA 
GAAAGCTTTG CCTCCGTTAC TGTGCGTGAT GATGTTGATA TCACAAGAAC AAACCTTGAA 
TTTCTACAGG ATCAGTTCAA CAGGATCACA ACACACATCC TACATTGTGC AGATTCCACA 
TTCGGACAGC GGCTTCTTGA GATGTGTAAC CAGTCTCTCT TTGAATGGCT TGCTCTCAAC 
CTGTATTGCC TCCGAGGGGA CCAGAGTGCT CTCACATCTG TCATTAATGA GAGAATTCGA 
AGACTCTCTC TAGATGTTTC ACCTGTGCTT GTCTCGTGGG TGACGTCTGT CCTGTCCCTC 
CGTCTTCAAG TGCTGTTGGG GCAGATGCCT GTGACTGAGG GCGAGATACA GAGACATATA 
CGGAGAGTGG GAGATGTTCC GCAGGCTCCT GAAGCATCAT CTCAGGATCA ACCTATGGAA 
ACCACACCTG TTGATTGTCA GCAGAATGGT GCTGCCTCTC CAGTTCCTGC CACCACAGTG 
GAGGAGGTGC TTTTCCTCCC CCCTCAAAGT TCAGTACCCA CCATATGCAC AGATTCTGAG 
CACCCAACCC AAGAAGATAC CGGATCTGAG CAATGGGCAG CATCTGTGCC CCCAGAATGG 
GTTCCTGTAA TCAGACAAGA TATGCAGAAC CAGCGGAAGA TGAAACAGCA GCCACCCTTG 
AGTGATGCCT ACCTGAGTGG CATGCCTGCA AAGCGCCGCA AAACAATGCA AGGTGAAGGG 
CCACACTTGT CCCTCTCTGA AGCTGTGAGC CGTGCAATGA AAGCCACAGG AGCCAAACCA 
GAGAGCAGTA CAGACTGTGT TAGGAGGGAG CTGGATAACA GTGAAGCACA AGGAAGGTAC 
AGAGAACAGT TGTGTCAGGA TATCCAGAAC ATCCTCCAAG ACAATGAGTC CTACAGTGCC 
CAGAGATTCC CCAACACACA AAGGGCATTC AGAGGGGACC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A conserved island of BAG6/Scythe is related to ubiquitin domains and participates in short hydrophobicity recognition.
The FEBS journal283(4)662-77(2016 Feb)
Tanaka H,Takahashi T,Xie Y,Minami R,Yanagi Y,Hayashishita M,Suzuki R,Yokota N,Shimada M,Mizushima T,Kuwabara N,Kato R,Kawahara H

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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bag6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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