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rasgrf2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol rasgrf2
Entrez Gene ID 100486502
Full Name Ras protein specific guanine nucleotide releasing factor 2
Synonyms grf2,ras-grf2
General protein information
Preferred Names

Ras protein specific guanine nucleotide releasing factor 2

Names

ras-specific guanine nucleotide-releasing factor 2
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_031893539.1 XP_031749399.1 ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_002942362.4 XP_002942408.2 ras-specific guanine nucleotide-releasing factor 2
XM_002942362.5 XP_002942408.2 ras-specific guanine nucleotide-releasing factor 2 isoform X1

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) RASGRF2 XP_005618219.1
Danio rerio (zebrafish) rasgrf2b NP_001121705.1
Pan troglodytes (chimpanzee) RASGRF2 XP_517672.2
Xenopus tropicalis (tropical clawed frog) rasgrf2 XP_002942408.2
Homo sapiens (human) RASGRF2 NP_008840.1
Mus musculus (house mouse) Rasgrf2 NP_033053.2
Rattus norvegicus (Norway rat) Rasgrf2 NP_446173.1
Gallus gallus (chicken) RASGRF2 XP_004949517.1
Bos taurus (cattle) RASGRF2 XP_002689419.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001238 XP_321917.5

Gene Ontology

The following rasgrf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rasgrf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa18810 XM_002942362.5
Latest version!		 Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OXa33635 XM_031893539.1
Latest version!		 Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OXa18810 XM_002942362.4 Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa18810
Clone ID Related Accession (Same CDS sequence) XM_002942362.4 , XM_002942362.5
Accession Version XM_002942362.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002942362.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC 
GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG 
AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT 
GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT 
CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG 
GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG 
CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG 
CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC 
CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA 
GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA 
TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT 
AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA 
CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA 
AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA 
ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC 
TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA 
TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG 
CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC 
ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT 
GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG 
GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA 
ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT 
ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA 
TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT 
TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG 
ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG 
TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT 
ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC 
CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC 
AAAGTTACAG TCCCACACAT GATCAGATCA GATGCACGAT TACATAAAGA TGATGTGGAT 
ATCTGCTTTA GCAGAACACT GAACTCGTGC AAAGTCCCTC AGATACGATA TGCAAGTGTG 
GATCGCCTGT TGGAGAGGTT AACTGACTTG CGCTTCCTTA GCATTGATTT TCTCAACACA 
TTCCTACACA CCTATCGGAT ATTTACTGCT GCATTTGTGG TGCTTGAGAA GCTTGCTGAT 
ATTTACAAAA GACCCTTCAC ATCAATCCCT GTCAGATCCC TGGAACTGTT TTTTGCTACA 
AGCCAAAACC TCAGTGATGG GAAATCACCA CATCTCTGTC GCAAGTTCTC CTCACCACCA 
CCCTTGTCTC TTTCAAGAAC CTCTTCTCCA ATCAGGGCCA GGAAACTATC ACTTAATTCT 
CCACTGAACT CAAGAATTGG AGCTTTGGAT TTATCCACTT CATCTAGTGC AAGCAGCCCT 
ACCTCTGTTT TCAGCCCTGC CACTTCTCCA CCTCCCACAA CTGCTAAAGT ACCTCTTGAT 
CTCAGTAAAG GACTCTCCTC TCCAGAGCAA AGTCCCGGAT CCATTGATGA GAATTCTGAA 
AACCCTCGTA TTGATTTGTG CAACAAATTG AGAAGAAGTA TTCGAAGAGC TGCGGTTTTA 
GAATCTGTTT CACTGGATAG GACCATTCCT GAGAGCAGCC AGTCTACAGA TACAGCTGAA 
AATTCACCAT GTAGGTCCCC CTCAACTCCG CGCCATCTCC GGTACCGCCC AACAGGAGTG 
CAGGCATCTG ACAACCAGCG TGGATGTTCT GTCTCCCCAG CCTCTGCCTT TGCCATTGCT 
ACAGCTGGAG CTGGTCATGG AAGTCCCCCA GGCTTTAATA ACATGGAGAG AATATGCGAC 
AAGGAGTTTA TAATCCGCAG AGCTGCAACA AACCGAGTAT TGAATGTTCT TCGTCACTGG 
GTCTCTAAGC ATGCACAGGA TTTTGAGTTA AACCATGAAA TAAAAATGAA TGTTGTCAAT 
TTGCTGGAAG ACGTTCTTCG TGACCCTGAC CTGCTACCCC AAGAGAGAAA AGCAGCTACT 
AACATATTAA GTGCCCTATC TCAGGAGGAA CAAGATGACT GCCACCTAGT CTTGGAGGAC 
ATTATAAACA TGTCAGAGAG TCCCAGCTCA GAGTGCCTGG AAACCCTATC TGCTATGGAA 
CTGGCAGAGC AGATAACCCT CTTAGATCAC ATAGTGTTTA GAAGCATACC ATACCAAGAG 
TTTCTTGGTC AAGGTTGGAT GAAACCAGAC AAATCTGAGA GGACGCCATA TATAATGAAA 
ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CGGAGATCAT GAAACACCCT 
GATGTCCCAT CCAGAGCAAG CTCAATTGAG AAGTGGGTAG TGGTAGCGGA TATATGCCGA 
TGTATGCACA ATTACAATGG AGTGCTGGAG ATCACATCCG CCTTGAACAG AAGTGCTGTC 
TACAGACTCA AGAAAACTTG GGCTAAAGTT TCTAAACAGA CAAAGGCTCT AATGGATAAG 
CTTCAAAAGA CTGTATCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC CCTCAAAAAC 
TGTAACCCCC CTTCAGTGCC CTACCTTGGA ATGTATTTGA CCGACCTTGC ATTTATAGAA 
GAGGGTACCC CAAACTTCAC TGAGGAGGGG CTTGTAAATT TTTCTAAAAT GCGAATGATA 
TCGCATATAA TTAGAGAAAT ACGGCAATTC CAGCAGACCC CTTACCGCAT AGAGCATCAG 
CCTAAGGTTA CTCAGTTTCT TCTGAACAAG TCTCGCATTC TGGATGAAGA TAATCTATAT 
GAATTGTCAT TGCGAATTGA ACCGCGTCTC CCAGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002942408.2
CDS913..4611
Translation

Target ORF information:

RefSeq Version XM_002942362.5
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002942362.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC 
GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG 
AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT 
GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT 
CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG 
GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG 
CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG 
CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC 
CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA 
GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA 
TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT 
AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA 
CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA 
AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA 
ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC 
TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA 
TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG 
CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC 
ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT 
GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG 
GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA 
ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT 
ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA 
TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT 
TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG 
ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG 
TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT 
ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC 
CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC 
AAAGTTACAG TCCCACACAT GATCAGATCA GATGCACGAT TACATAAAGA TGATGTGGAT 
ATCTGCTTTA GCAGAACACT GAACTCGTGC AAAGTCCCTC AGATACGATA TGCAAGTGTG 
GATCGCCTGT TGGAGAGGTT AACTGACTTG CGCTTCCTTA GCATTGATTT TCTCAACACA 
TTCCTACACA CCTATCGGAT ATTTACTGCT GCATTTGTGG TGCTTGAGAA GCTTGCTGAT 
ATTTACAAAA GACCCTTCAC ATCAATCCCT GTCAGATCCC TGGAACTGTT TTTTGCTACA 
AGCCAAAACC TCAGTGATGG GAAATCACCA CATCTCTGTC GCAAGTTCTC CTCACCACCA 
CCCTTGTCTC TTTCAAGAAC CTCTTCTCCA ATCAGGGCCA GGAAACTATC ACTTAATTCT 
CCACTGAACT CAAGAATTGG AGCTTTGGAT TTATCCACTT CATCTAGTGC AAGCAGCCCT 
ACCTCTGTTT TCAGCCCTGC CACTTCTCCA CCTCCCACAA CTGCTAAAGT ACCTCTTGAT 
CTCAGTAAAG GACTCTCCTC TCCAGAGCAA AGTCCCGGAT CCATTGATGA GAATTCTGAA 
AACCCTCGTA TTGATTTGTG CAACAAATTG AGAAGAAGTA TTCGAAGAGC TGCGGTTTTA 
GAATCTGTTT CACTGGATAG GACCATTCCT GAGAGCAGCC AGTCTACAGA TACAGCTGAA 
AATTCACCAT GTAGGTCCCC CTCAACTCCG CGCCATCTCC GGTACCGCCC AACAGGAGTG 
CAGGCATCTG ACAACCAGCG TGGATGTTCT GTCTCCCCAG CCTCTGCCTT TGCCATTGCT 
ACAGCTGGAG CTGGTCATGG AAGTCCCCCA GGCTTTAATA ACATGGAGAG AATATGCGAC 
AAGGAGTTTA TAATCCGCAG AGCTGCAACA AACCGAGTAT TGAATGTTCT TCGTCACTGG 
GTCTCTAAGC ATGCACAGGA TTTTGAGTTA AACCATGAAA TAAAAATGAA TGTTGTCAAT 
TTGCTGGAAG ACGTTCTTCG TGACCCTGAC CTGCTACCCC AAGAGAGAAA AGCAGCTACT 
AACATATTAA GTGCCCTATC TCAGGAGGAA CAAGATGACT GCCACCTAGT CTTGGAGGAC 
ATTATAAACA TGTCAGAGAG TCCCAGCTCA GAGTGCCTGG AAACCCTATC TGCTATGGAA 
CTGGCAGAGC AGATAACCCT CTTAGATCAC ATAGTGTTTA GAAGCATACC ATACCAAGAG 
TTTCTTGGTC AAGGTTGGAT GAAACCAGAC AAATCTGAGA GGACGCCATA TATAATGAAA 
ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CGGAGATCAT GAAACACCCT 
GATGTCCCAT CCAGAGCAAG CTCAATTGAG AAGTGGGTAG TGGTAGCGGA TATATGCCGA 
TGTATGCACA ATTACAATGG AGTGCTGGAG ATCACATCCG CCTTGAACAG AAGTGCTGTC 
TACAGACTCA AGAAAACTTG GGCTAAAGTT TCTAAACAGA CAAAGGCTCT AATGGATAAG 
CTTCAAAAGA CTGTATCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC CCTCAAAAAC 
TGTAACCCCC CTTCAGTGCC CTACCTTGGA ATGTATTTGA CCGACCTTGC ATTTATAGAA 
GAGGGTACCC CAAACTTCAC TGAGGAGGGG CTTGTAAATT TTTCTAAAAT GCGAATGATA 
TCGCATATAA TTAGAGAAAT ACGGCAATTC CAGCAGACCC CTTACCGCAT AGAGCATCAG 
CCTAAGGTTA CTCAGTTTCT TCTGAACAAG TCTCGCATTC TGGATGAAGA TAATCTATAT 
GAATTGTCAT TGCGAATTGA ACCGCGTCTC CCAGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa33635
Clone ID Related Accession (Same CDS sequence) XM_031893539.1
Accession Version XM_031893539.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC 
GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG 
AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT 
GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT 
CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG 
GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG 
CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG 
CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC 
CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA 
GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA 
TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT 
AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA 
CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA 
AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA 
ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC 
TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA 
TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG 
CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC 
ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT 
GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG 
GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA 
ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT 
ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA 
TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT 
TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG 
ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG 
TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT 
ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC 
CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC 
AAAGTTACAG TCCCACACAT GATCAGAACA CTGAACTCGT GCAAAGTCCC TCAGATACGA 
TATGCAAGTG TGGATCGCCT GTTGGAGAGG TTAACTGACT TGCGCTTCCT TAGCATTGAT 
TTTCTCAACA CATTCCTACA CACCTATCGG ATATTTACTG CTGCATTTGT GGTGCTTGAG 
AAGCTTGCTG ATATTTACAA AAGACCCTTC ACATCAATCC CTGTCAGATC CCTGGAACTG 
TTTTTTGCTA CAAGCCAAAA CCTCAGTGAT GGGAAATCAC CACATCTCTG TCGCAAGTTC 
TCCTCACCAC CACCCTTGTC TCTTTCAAGA ACCTCTTCTC CAATCAGGGC CAGGAAACTA 
TCACTTAATT CTCCACTGAA CTCAAGAATT GGAGCTTTGG ATTTATCCAC TTCATCTAGT 
GCAAGCAGCC CTACCTCTGT TTTCAGCCCT GCCACTTCTC CACCTCCCAC AACTGCTAAA 
GTACCTCTTG ATCTCAGTAA AGGACTCTCC TCTCCAGAGC AAAGTCCCGG ATCCATTGAT 
GAGAATTCTG AAAACCCTCG TATTGATTTG TGCAACAAAT TGAGAAGAAG TATTCGAAGA 
GCTGCGGTTT TAGAATCTGT TTCACTGGAT AGGACCATTC CTGAGAGCAG CCAGTCTACA 
GATACAGCTG AAAATTCACC ATGTAGGTCC CCCTCAACTC CGCGCCATCT CCGGTACCGC 
CCAACAGGAG TGCAGGCATC TGACAACCAG CGTGGATGTT CTGTCTCCCC AGCCTCTGCC 
TTTGCCATTG CTACAGCTGG AGCTGGTCAT GGAAGTCCCC CAGGCTTTAA TAACATGGAG 
AGAATATGCG ACAAGGAGTT TATAATCCGC AGAGCTGCAA CAAACCGAGT ATTGAATGTT 
CTTCGTCACT GGGTCTCTAA GCATGCACAG GATTTTGAGT TAAACCATGA AATAAAAATG 
AATGTTGTCA ATTTGCTGGA AGACGTTCTT CGTGACCCTG ACCTGCTACC CCAAGAGAGA 
AAAGCAGCTA CTAACATATT AAGTGCCCTA TCTCAGGAGG AACAAGATGA CTGCCACCTA 
GTCTTGGAGG ACATTATAAA CATGTCAGAG AGTCCCAGCT CAGAGTGCCT GGAAACCCTA 
TCTGCTATGG AACTGGCAGA GCAGATAACC CTCTTAGATC ACATAGTGTT TAGAAGCATA 
CCATACCAAG AGTTTCTTGG TCAAGGTTGG ATGAAACCAG ACAAATCTGA GAGGACGCCA 
TATATAATGA AAACTAGTCA ACATTTTAAT GATATGAGTA ACCTTGTTGC CTCGGAGATC 
ATGAAACACC CTGATGTCCC ATCCAGAGCA AGCTCAATTG AGAAGTGGGT AGTGGTAGCG 
GATATATGCC GATGTATGCA CAATTACAAT GGAGTGCTGG AGATCACATC CGCCTTGAAC 
AGAAGTGCTG TCTACAGACT CAAGAAAACT TGGGCTAAAG TTTCTAAACA GACAAAGGCT 
CTAATGGATA AGCTTCAAAA GACTGTATCA TCTGAAGGAA GATTTAAAAA TCTTAGAGAA 
ACCCTCAAAA ACTGTAACCC CCCTTCAGTG CCCTACCTTG GAATGTATTT GACCGACCTT 
GCATTTATAG AAGAGGGTAC CCCAAACTTC ACTGAGGAGG GGCTTGTAAA TTTTTCTAAA 
ATGCGAATGA TATCGCATAT AATTAGAGAA ATACGGCAAT TCCAGCAGAC CCCTTACCGC 
ATAGAGCATC AGCCTAAGGT TACTCAGTTT CTTCTGAACA AGTCTCGCAT TCTGGATGAA 
GATAATCTAT ATGAATTGTC ATTGCGAATT GAACCGCGTC TCCCAGCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_031749399.1
CDS914..4564
Translation

Target ORF information:

RefSeq Version XM_031893539.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_031893539.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC 
GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG 
AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT 
GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT 
CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG 
GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG 
CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG 
CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC 
CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA 
GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA 
TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT 
AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA 
CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA 
AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA 
ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC 
TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA 
TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG 
CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC 
ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT 
GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG 
GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA 
ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT 
ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA 
TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT 
TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG 
ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG 
TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT 
ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC 
CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC 
AAAGTTACAG TCCCACACAT GATCAGAACA CTGAACTCGT GCAAAGTCCC TCAGATACGA 
TATGCAAGTG TGGATCGCCT GTTGGAGAGG TTAACTGACT TGCGCTTCCT TAGCATTGAT 
TTTCTCAACA CATTCCTACA CACCTATCGG ATATTTACTG CTGCATTTGT GGTGCTTGAG 
AAGCTTGCTG ATATTTACAA AAGACCCTTC ACATCAATCC CTGTCAGATC CCTGGAACTG 
TTTTTTGCTA CAAGCCAAAA CCTCAGTGAT GGGAAATCAC CACATCTCTG TCGCAAGTTC 
TCCTCACCAC CACCCTTGTC TCTTTCAAGA ACCTCTTCTC CAATCAGGGC CAGGAAACTA 
TCACTTAATT CTCCACTGAA CTCAAGAATT GGAGCTTTGG ATTTATCCAC TTCATCTAGT 
GCAAGCAGCC CTACCTCTGT TTTCAGCCCT GCCACTTCTC CACCTCCCAC AACTGCTAAA 
GTACCTCTTG ATCTCAGTAA AGGACTCTCC TCTCCAGAGC AAAGTCCCGG ATCCATTGAT 
GAGAATTCTG AAAACCCTCG TATTGATTTG TGCAACAAAT TGAGAAGAAG TATTCGAAGA 
GCTGCGGTTT TAGAATCTGT TTCACTGGAT AGGACCATTC CTGAGAGCAG CCAGTCTACA 
GATACAGCTG AAAATTCACC ATGTAGGTCC CCCTCAACTC CGCGCCATCT CCGGTACCGC 
CCAACAGGAG TGCAGGCATC TGACAACCAG CGTGGATGTT CTGTCTCCCC AGCCTCTGCC 
TTTGCCATTG CTACAGCTGG AGCTGGTCAT GGAAGTCCCC CAGGCTTTAA TAACATGGAG 
AGAATATGCG ACAAGGAGTT TATAATCCGC AGAGCTGCAA CAAACCGAGT ATTGAATGTT 
CTTCGTCACT GGGTCTCTAA GCATGCACAG GATTTTGAGT TAAACCATGA AATAAAAATG 
AATGTTGTCA ATTTGCTGGA AGACGTTCTT CGTGACCCTG ACCTGCTACC CCAAGAGAGA 
AAAGCAGCTA CTAACATATT AAGTGCCCTA TCTCAGGAGG AACAAGATGA CTGCCACCTA 
GTCTTGGAGG ACATTATAAA CATGTCAGAG AGTCCCAGCT CAGAGTGCCT GGAAACCCTA 
TCTGCTATGG AACTGGCAGA GCAGATAACC CTCTTAGATC ACATAGTGTT TAGAAGCATA 
CCATACCAAG AGTTTCTTGG TCAAGGTTGG ATGAAACCAG ACAAATCTGA GAGGACGCCA 
TATATAATGA AAACTAGTCA ACATTTTAAT GATATGAGTA ACCTTGTTGC CTCGGAGATC 
ATGAAACACC CTGATGTCCC ATCCAGAGCA AGCTCAATTG AGAAGTGGGT AGTGGTAGCG 
GATATATGCC GATGTATGCA CAATTACAAT GGAGTGCTGG AGATCACATC CGCCTTGAAC 
AGAAGTGCTG TCTACAGACT CAAGAAAACT TGGGCTAAAG TTTCTAAACA GACAAAGGCT 
CTAATGGATA AGCTTCAAAA GACTGTATCA TCTGAAGGAA GATTTAAAAA TCTTAGAGAA 
ACCCTCAAAA ACTGTAACCC CCCTTCAGTG CCCTACCTTG GAATGTATTT GACCGACCTT 
GCATTTATAG AAGAGGGTAC CCCAAACTTC ACTGAGGAGG GGCTTGTAAA TTTTTCTAAA 
ATGCGAATGA TATCGCATAT AATTAGAGAA ATACGGCAAT TCCAGCAGAC CCCTTACCGC 
ATAGAGCATC AGCCTAAGGT TACTCAGTTT CTTCTGAACA AGTCTCGCAT TCTGGATGAA 
GATAATCTAT ATGAATTGTC ATTGCGAATT GAACCGCGTC TCCCAGCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa18810
Clone ID Related Accession (Same CDS sequence) XM_002942362.4 , XM_002942362.5
Accession Version XM_002942362.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product ras-specific guanine nucleotide-releasing factor 2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002942362.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC 
GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG 
AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT 
GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT 
CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG 
GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG 
CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG 
CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC 
CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA 
GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA 
TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT 
AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA 
CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA 
AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA 
ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC 
TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA 
TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG 
CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC 
ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT 
GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG 
GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA 
ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT 
ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA 
TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT 
TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG 
ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG 
TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT 
ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC 
CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC 
AAAGTTACAG TCCCACACAT GATCAGATCA GATGCACGAT TACATAAAGA TGATGTGGAT 
ATCTGCTTTA GCAGAACACT GAACTCGTGC AAAGTCCCTC AGATACGATA TGCAAGTGTG 
GATCGCCTGT TGGAGAGGTT AACTGACTTG CGCTTCCTTA GCATTGATTT TCTCAACACA 
TTCCTACACA CCTATCGGAT ATTTACTGCT GCATTTGTGG TGCTTGAGAA GCTTGCTGAT 
ATTTACAAAA GACCCTTCAC ATCAATCCCT GTCAGATCCC TGGAACTGTT TTTTGCTACA 
AGCCAAAACC TCAGTGATGG GAAATCACCA CATCTCTGTC GCAAGTTCTC CTCACCACCA 
CCCTTGTCTC TTTCAAGAAC CTCTTCTCCA ATCAGGGCCA GGAAACTATC ACTTAATTCT 
CCACTGAACT CAAGAATTGG AGCTTTGGAT TTATCCACTT CATCTAGTGC AAGCAGCCCT 
ACCTCTGTTT TCAGCCCTGC CACTTCTCCA CCTCCCACAA CTGCTAAAGT ACCTCTTGAT 
CTCAGTAAAG GACTCTCCTC TCCAGAGCAA AGTCCCGGAT CCATTGATGA GAATTCTGAA 
AACCCTCGTA TTGATTTGTG CAACAAATTG AGAAGAAGTA TTCGAAGAGC TGCGGTTTTA 
GAATCTGTTT CACTGGATAG GACCATTCCT GAGAGCAGCC AGTCTACAGA TACAGCTGAA 
AATTCACCAT GTAGGTCCCC CTCAACTCCG CGCCATCTCC GGTACCGCCC AACAGGAGTG 
CAGGCATCTG ACAACCAGCG TGGATGTTCT GTCTCCCCAG CCTCTGCCTT TGCCATTGCT 
ACAGCTGGAG CTGGTCATGG AAGTCCCCCA GGCTTTAATA ACATGGAGAG AATATGCGAC 
AAGGAGTTTA TAATCCGCAG AGCTGCAACA AACCGAGTAT TGAATGTTCT TCGTCACTGG 
GTCTCTAAGC ATGCACAGGA TTTTGAGTTA AACCATGAAA TAAAAATGAA TGTTGTCAAT 
TTGCTGGAAG ACGTTCTTCG TGACCCTGAC CTGCTACCCC AAGAGAGAAA AGCAGCTACT 
AACATATTAA GTGCCCTATC TCAGGAGGAA CAAGATGACT GCCACCTAGT CTTGGAGGAC 
ATTATAAACA TGTCAGAGAG TCCCAGCTCA GAGTGCCTGG AAACCCTATC TGCTATGGAA 
CTGGCAGAGC AGATAACCCT CTTAGATCAC ATAGTGTTTA GAAGCATACC ATACCAAGAG 
TTTCTTGGTC AAGGTTGGAT GAAACCAGAC AAATCTGAGA GGACGCCATA TATAATGAAA 
ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CGGAGATCAT GAAACACCCT 
GATGTCCCAT CCAGAGCAAG CTCAATTGAG AAGTGGGTAG TGGTAGCGGA TATATGCCGA 
TGTATGCACA ATTACAATGG AGTGCTGGAG ATCACATCCG CCTTGAACAG AAGTGCTGTC 
TACAGACTCA AGAAAACTTG GGCTAAAGTT TCTAAACAGA CAAAGGCTCT AATGGATAAG 
CTTCAAAAGA CTGTATCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC CCTCAAAAAC 
TGTAACCCCC CTTCAGTGCC CTACCTTGGA ATGTATTTGA CCGACCTTGC ATTTATAGAA 
GAGGGTACCC CAAACTTCAC TGAGGAGGGG CTTGTAAATT TTTCTAAAAT GCGAATGATA 
TCGCATATAA TTAGAGAAAT ACGGCAATTC CAGCAGACCC CTTACCGCAT AGAGCATCAG 
CCTAAGGTTA CTCAGTTTCT TCTGAACAAG TCTCGCATTC TGGATGAAGA TAATCTATAT 
GAATTGTCAT TGCGAATTGA ACCGCGTCTC CCAGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002942408.2
CDS1490..5188
Translation

Target ORF information:

RefSeq Version XM_002942362.4
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis Ras protein specific guanine nucleotide releasing factor 2 (rasgrf2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002942362.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA CTGTGCGCCT TAACGAGGGC CACACTCTGC ACCTGGCCCT GCTGGCCCGC 
AAGGAGGGGA GCAAGAGGGG CGCCCTCAGC AAGAAAGCCA CGGAGACCAA CAGGTGGCAC 
GAAAAGTGGT TTGCCCTCTA CCAGAATGTC CTCTTCTACT TCGAGTCGGA GCAGAGCGGG 
AGACCCTCGG GGGTTTATTT GCTGGAGGGG TGCAGTTGTG AGAGGGTCAT AGCACCCATT 
GTGGCCAGCG GGGGCAAAGA CACCATGGAG AAGCAGCATT ACTTCACTGT TACCTTTGGT 
CATGAAGCAC AGAAGCCTTT GGAGCTACGC TGTGAAGAGG AACCTGACCG AGATGAATGG 
GTGGAAGCCA TACACCAAGC GAGTTACTCT GATGTCCTGA TAGAGAGAGA AGTCCTGATG 
CAGAAGTATA TTCATTTGGT GCAAATTGTA GAAACCGAGA AAATTGCTGC CAATCAACTG 
CGACAACAGC TTGAAGATCA AGATACTGAA ATAGAAAGGC TTAAATCCGA GATTGTAGCC 
CTTAATAAAA CAAAGGAAAG AATGCGTCCC TATCAAATGA ATCAAGAGGA CGAGGATCCA 
GACATTAAGA AGATAAAAAA GGTTCAAAGT TTTATGCGTG GATGGCTTTG CAGGAGAAAA 
TGGAAAACAA TTGTGCAGGA CTATATCTGT TCCCCCCATG CTGAAAGCAT GAGGAAACGT 
AACCAAATTG TTTTTAATAT GGTGGAAGCA GAGGCAGAAT ATGTGCATCA GCTTTATATA 
CTTGTCAACT GCTTCCTTAG GCCCCTCAGA ATGGCAGCCA GCTCCAAGAA ACCCCCAATA 
AGCCATGATG ATGTAAGCAG CATCTTCCTA AACAGTGAAA CTATAATGTT TCTTCATGAA 
ATATTCCACC AGGGATTGAA AGCAAGAATT AATAATTGGC CTACTTTGAT TTTAGCTGAC 
TTATTCGATA TCCTGCTACC AATGTTGAAC ATCTACCAAG AGTTTGTTCG TAATCATCAA 
TATAGTCTGC AAGTGCTGGC GAACTGTAAA CAAAACAGAG ATTTTGACAA ACTCTTGAAG 
CAATATGAAA CCAATCCAGC TTGTGAAGGG AGGATGCTCG AGACATTCCT TACCTATCCC 
ATGTTTCAGA TCCCTCGGTA CATCATAACA CTCCATGAAC TTCTTGCCCA CACACCCCAT 
GAGCATGTAG AGAGAAAAAG CTTAGACTTT GCACAATCAA AACTGGAAGA GCTGTCAAGG 
GTTATGCACG ATGAAGTCAG TGACACAGAG AACATTCGCA AGAACCTCTC CATTGAGAGA 
ATGATAGTGG AAGGCTGTGA TATTTTATTA GATACAAGCC AGACATTTGT ACGCCAAGGT 
ACTCTTATTC AGGTTCCTTC TGTGGAAAGG GGGAAACTTA GTAAAGTCCG CCTGGGCCCA 
TTGTCACTGA AAAGAGAAGG GGAACGACAA TGCTTCTTAT TTACAAAGCA TTTTTTAATT 
TGCACTAGAA GCTCAGGGGG GAAACTCCAT TTACTCAAGA CAGGGGGAGT TCTGTCCTTG 
ATAGAATGTA CATTGATTGA AGAGCCAGAT CTGGGTGAAG ATGACACAAA GGGTACAGGG 
TATGTTTTTG GACATTTGGA TTTCAAGATT GTCATAGAAC CAGTGGATTC CCCTTCATTT 
ACTGTGGTCC TTCTTGCACC ATCACGTCAG GAAAAAGCTG CTTGGACAAG TGATATCAGC 
CAGTGTGTAG AGAACATAAG GTGCAATGGC TTGATGAGTA ATGTATTTGA GGAGAACTCC 
AAAGTTACAG TCCCACACAT GATCAGATCA GATGCACGAT TACATAAAGA TGATGTGGAT 
ATCTGCTTTA GCAGAACACT GAACTCGTGC AAAGTCCCTC AGATACGATA TGCAAGTGTG 
GATCGCCTGT TGGAGAGGTT AACTGACTTG CGCTTCCTTA GCATTGATTT TCTCAACACA 
TTCCTACACA CCTATCGGAT ATTTACTGCT GCATTTGTGG TGCTTGAGAA GCTTGCTGAT 
ATTTACAAAA GACCCTTCAC ATCAATCCCT GTCAGATCCC TGGAACTGTT TTTTGCTACA 
AGCCAAAACC TCAGTGATGG GAAATCACCA CATCTCTGTC GCAAGTTCTC CTCACCACCA 
CCCTTGTCTC TTTCAAGAAC CTCTTCTCCA ATCAGGGCCA GGAAACTATC ACTTAATTCT 
CCACTGAACT CAAGAATTGG AGCTTTGGAT TTATCCACTT CATCTAGTGC AAGCAGCCCT 
ACCTCTGTTT TCAGCCCTGC CACTTCTCCA CCTCCCACAA CTGCTAAAGT ACCTCTTGAT 
CTCAGTAAAG GACTCTCCTC TCCAGAGCAA AGTCCCGGAT CCATTGATGA GAATTCTGAA 
AACCCTCGTA TTGATTTGTG CAACAAATTG AGAAGAAGTA TTCGAAGAGC TGCGGTTTTA 
GAATCTGTTT CACTGGATAG GACCATTCCT GAGAGCAGCC AGTCTACAGA TACAGCTGAA 
AATTCACCAT GTAGGTCCCC CTCAACTCCG CGCCATCTCC GGTACCGCCC AACAGGAGTG 
CAGGCATCTG ACAACCAGCG TGGATGTTCT GTCTCCCCAG CCTCTGCCTT TGCCATTGCT 
ACAGCTGGAG CTGGTCATGG AAGTCCCCCA GGCTTTAATA ACATGGAGAG AATATGCGAC 
AAGGAGTTTA TAATCCGCAG AGCTGCAACA AACCGAGTAT TGAATGTTCT TCGTCACTGG 
GTCTCTAAGC ATGCACAGGA TTTTGAGTTA AACCATGAAA TAAAAATGAA TGTTGTCAAT 
TTGCTGGAAG ACGTTCTTCG TGACCCTGAC CTGCTACCCC AAGAGAGAAA AGCAGCTACT 
AACATATTAA GTGCCCTATC TCAGGAGGAA CAAGATGACT GCCACCTAGT CTTGGAGGAC 
ATTATAAACA TGTCAGAGAG TCCCAGCTCA GAGTGCCTGG AAACCCTATC TGCTATGGAA 
CTGGCAGAGC AGATAACCCT CTTAGATCAC ATAGTGTTTA GAAGCATACC ATACCAAGAG 
TTTCTTGGTC AAGGTTGGAT GAAACCAGAC AAATCTGAGA GGACGCCATA TATAATGAAA 
ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CGGAGATCAT GAAACACCCT 
GATGTCCCAT CCAGAGCAAG CTCAATTGAG AAGTGGGTAG TGGTAGCGGA TATATGCCGA 
TGTATGCACA ATTACAATGG AGTGCTGGAG ATCACATCCG CCTTGAACAG AAGTGCTGTC 
TACAGACTCA AGAAAACTTG GGCTAAAGTT TCTAAACAGA CAAAGGCTCT AATGGATAAG 
CTTCAAAAGA CTGTATCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC CCTCAAAAAC 
TGTAACCCCC CTTCAGTGCC CTACCTTGGA ATGTATTTGA CCGACCTTGC ATTTATAGAA 
GAGGGTACCC CAAACTTCAC TGAGGAGGGG CTTGTAAATT TTTCTAAAAT GCGAATGATA 
TCGCATATAA TTAGAGAAAT ACGGCAATTC CAGCAGACCC CTTACCGCAT AGAGCATCAG 
CCTAAGGTTA CTCAGTTTCT TCTGAACAAG TCTCGCATTC TGGATGAAGA TAATCTATAT 
GAATTGTCAT TGCGAATTGA ACCGCGTCTC CCAGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed