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RASGRF2 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol RASGRF2
Entrez Gene ID 5924
Full Name Ras protein specific guanine nucleotide releasing factor 2
Synonyms GRF2,RAS-GRF2
General protein information
Preferred Names

Ras protein specific guanine nucleotide releasing factor 2

Names

ras-specific guanine nucleotide-releasing factor 2
ras guanine nucleotide exchange factor 2
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

5

5q14.1
Summary RAS GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. This gene encodes a calcium-regulated nucleotide exchange factor activating both RAS and RAS-related protein, RAC1, through the exchange of bound GDP for GTP, thereby, coordinating the signaling of distinct mitogen-activated protein kinase pathways. [provided by RefSeq, Oct 2011].
Disorder MIM:

606614


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017009682.2 XP_016865171.1 ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_017009683.1 XP_016865172.1 ras-specific guanine nucleotide-releasing factor 2 isoform X4
NM_006909.2 NP_008840.1 ras-specific guanine nucleotide-releasing factor 2
XM_024446142.1 XP_024301910.1 ras-specific guanine nucleotide-releasing factor 2 isoform X5
XM_017009682.1 XP_016865171.1 ras-specific guanine nucleotide-releasing factor 2 isoform X1
NM_006909.3 NP_008840.1 ras-specific guanine nucleotide-releasing factor 2
XM_005248565.1 XP_005248622.1 ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_024446141.1 XP_024301909.1 ras-specific guanine nucleotide-releasing factor 2 isoform X3

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) RASGRF2 XP_005618219.1
Danio rerio (zebrafish) rasgrf2b NP_001121705.1
Pan troglodytes (chimpanzee) RASGRF2 XP_517672.2
Xenopus tropicalis (tropical clawed frog) rasgrf2 XP_002942408.2
Homo sapiens (human) RASGRF2 NP_008840.1
Mus musculus (house mouse) Rasgrf2 NP_033053.2
Rattus norvegicus (Norway rat) Rasgrf2 NP_446173.1
Gallus gallus (chicken) RASGRF2 XP_004949517.1
Bos taurus (cattle) RASGRF2 XP_002689419.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001238 XP_321917.5

Gene Ontology


Bibliography

Related articles in PubMed

RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.
Wu RF, Liao C, Hatoum H, Fu G, Ochoa CD, Terada LS
Arteriosclerosis, thrombosis, and vascular biology37(1)98-107(2017 Jan)

A Single Nucleotide Polymorphism in the RASGRF2 Gene Is Associated with Alcoholic Liver Cirrhosis in Men.
Novo-Veleiro I, Cieza-Borrella C, Pastor I, Chamorro AJ, Laso FJ, Gonz?lez-Sarmiento R, Marcos M
PloS one11(12)e0168685(2016)

Common variants at 1p36 are associated with superior frontal gyrus volume.
Hashimoto R, Ikeda M, Yamashita F, Ohi K, Yamamori H, Yasuda Y, Fujimoto M, Fukunaga M, Nemoto K, Takahashi T, Tochigi M, Onitsuka T, Yamasue H, Matsuo K, Iidaka T, Iwata N, Suzuki M, Takeda M, Kasai K, Ozaki N
Translational psychiatry4e472(2014 Oct)

?Arrestin1 regulates the guanine nucleotide exchange factor RasGRF2 expression and the small GTPase Rac-mediated formation of membrane protrusion and cell motility.
Ma X, Espana-Serrano L, Kim WJ, Thayele Purayil H, Nie Z, Daaka Y
The Journal of biological chemistry289(19)13638-50(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endoplasmic reticulum stress triggers a localized signaling module on the ER surface involving Nox4-dependent calcium mobilization, which directs local Ras activation through ER-associated, calcium-responsive RasGRF.
Title: RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.

this genetic association study represents the first to show an association of the RASGRF2 G>A (rs26907) polymorphism with Alcoholic Liver Cirrhosis in men, particularly in the subgroup of patients with alcohol dependence. The findings suggest the potential relevance of the RAS gene family in alcoholism and Alcoholic Liver Cirrhosis.
Title: A Single Nucleotide Polymorphism in the RASGRF2 Gene Is Associated with Alcoholic Liver Cirrhosis in Men.

these results suggest that betaarrestin1 regulates rasgrf2 gene expression and Rac activation to affect membrane protrusion and cell migration and invasion.
Title: ?Arrestin1 regulates the guanine nucleotide exchange factor RasGRF2 expression and the small GTPase Rac-mediated formation of membrane protrusion and cell motility.

Case-control studies reveal malignant mesothelioma risk associated with variants in the SDK1, CRTAM and RASGRF2 genes.
Title: A genome-wide association study for malignant mesothelioma risk.

RASGRF2 has a role in the regulation of mesolimbic dopamine neuron activity, reward response, and alcohol use and abuse
Title: RASGRF2 regulates alcohol-induced reinforcement by influencing mesolimbic dopamine neuron activity and dopamine release.

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18424 NM_006909.3
Latest version!		 Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $624.50-$874.30
$1249.00
OHu39662 XM_005248565.1
Latest version!		 Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu105682 XM_024446141.1
Latest version!		 Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu105683 XM_024446142.1
Latest version!		 Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu95975 XM_017009683.1
Latest version!		 Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu95974 XM_017009682.2
Latest version!		 Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 15-17 $559.30
$799.00
View Old Accession Versions >>
OHu95974 XM_017009682.1 Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 15-17 $559.30
$799.00
OHu18424 NM_006909.2 Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $624.50-$874.30
$1249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu18424
Clone ID Related Accession (Same CDS sequence) NM_006909.2 , NM_006909.3
Accession Version NM_006909.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-27
Organism Homo sapiens(human)
Product ras-specific guanine nucleotide-releasing factor 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC136296.1, DA219722.1, DA230428.1, BX502901.1, BU430075.1, CR749239.1 and BC041953.1. This sequence is a reference standard in the RefSeqGene project. On Oct 8, 2011 this sequence version replaced NM_006909.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126112.1, SRR1803611.174237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
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301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGACAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGATTTCG AACTCAACAA TGAACTAAAG 
ATGAATGTCC TAAATTTGCT AGAAGAAGTT TTGCGAGACC CAGACCTTCT TCCCCAAGAA 
AGGAAAGCCG CCGCGAATAT CCTCAGGGCC CTTTCACAAG ATGACCAAGA TGACATCCAC 
CTAAAATTAG AGGATATAAT TCAAATGACT GACTGCATGA AGGCCGAATG CTTTGAGTCC 
TTGTCGGCCA TGGAGCTGGC AGAACAGATC ACCCTCCTGG ACCATGTCAT TTTCAGAAGC 
ATTCCCTACG AGGAGTTTCT TGGGCAGGGG TGGATGAAGC TGGATAAAAA CGAAAGAACT 
CCTTACATTA TGAAAACCAG CCAACACTTC AATGACATGA GTAACCTGGT GGCCTCCCAG 
ATAATGAACT ATGCTGATGT CAGCTCCCGT GCCAACGCCA TCGAGAAATG GGTGGCAGTG 
GCGGACATCT GCCGATGCCT GCACAACTAC AACGGCGTGC TGGAGATCAC CTCGGCCTTA 
AACAGAAGTG CCATCTACAG GCTGAAGAAA ACCTGGGCCA AGGTCTCTAA GCAGACAAAA 
GCTCTAATGG ACAAACTTCA AAAGACTGTT TCCTCTGAAG GAAGATTTAA AAATCTTAGA 
GAAACCCTTA AAAATTGTAA CCCTCCTGCA GTTCCTTATC TTGGGATGTA CTTGACAGAC 
CTGGCATTTA TTGAAGAAGG AACACCAAAC TTTACTGAGG AAGGCCTTGT CAATTTCTCC 
AAAATGAGAA TGATATCACA CATCATCAGA GAGATACGCC AGTTCCAGCA GACTTCCTAC 
AGAATAGATC ATCAGCCAAA GGTCGCACAG TACTTGCTTG ACAAAGACCT TATCATAGAT 
GAAGATACGC TATATGAGCT GTCACTAAAA ATTGAACCTC GACTCCCTGC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_008840.1
CDS51..3764
Translation

Target ORF information:

RefSeq Version NM_006909.2
Organism Homo sapiens(human)
Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006909.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGACAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGATTTCG AACTCAACAA TGAACTAAAG 
ATGAATGTCC TAAATTTGCT AGAAGAAGTT TTGCGAGACC CAGACCTTCT TCCCCAAGAA 
AGGAAAGCCG CCGCGAATAT CCTCAGGGCC CTTTCACAAG ATGACCAAGA TGACATCCAC 
CTAAAATTAG AGGATATAAT TCAAATGACT GACTGCATGA AGGCCGAATG CTTTGAGTCC 
TTGTCGGCCA TGGAGCTGGC AGAACAGATC ACCCTCCTGG ACCATGTCAT TTTCAGAAGC 
ATTCCCTACG AGGAGTTTCT TGGGCAGGGG TGGATGAAGC TGGATAAAAA CGAAAGAACT 
CCTTACATTA TGAAAACCAG CCAACACTTC AATGACATGA GTAACCTGGT GGCCTCCCAG 
ATAATGAACT ATGCTGATGT CAGCTCCCGT GCCAACGCCA TCGAGAAATG GGTGGCAGTG 
GCGGACATCT GCCGATGCCT GCACAACTAC AACGGCGTGC TGGAGATCAC CTCGGCCTTA 
AACAGAAGTG CCATCTACAG GCTGAAGAAA ACCTGGGCCA AGGTCTCTAA GCAGACAAAA 
GCTCTAATGG ACAAACTTCA AAAGACTGTT TCCTCTGAAG GAAGATTTAA AAATCTTAGA 
GAAACCCTTA AAAATTGTAA CCCTCCTGCA GTTCCTTATC TTGGGATGTA CTTGACAGAC 
CTGGCATTTA TTGAAGAAGG AACACCAAAC TTTACTGAGG AAGGCCTTGT CAATTTCTCC 
AAAATGAGAA TGATATCACA CATCATCAGA GAGATACGCC AGTTCCAGCA GACTTCCTAC 
AGAATAGATC ATCAGCCAAA GGTCGCACAG TACTTGCTTG ACAAAGACCT TATCATAGAT 
GAAGATACGC TATATGAGCT GTCACTAAAA ATTGAACCTC GACTCCCTGC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18424
Clone ID Related Accession (Same CDS sequence) NM_006909.2 , NM_006909.3
Accession Version NM_006909.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-24
Organism Homo sapiens(human)
Product ras-specific guanine nucleotide-releasing factor 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026427.4, BC136296.1, DA219722.1, DA230428.1, BX502901.1, BU430075.1, CR749239.1 and BC041953.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_006909.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.174237.1, SRR1803614.86111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265080.9/ ENSP00000265080.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGACAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGATTTCG AACTCAACAA TGAACTAAAG 
ATGAATGTCC TAAATTTGCT AGAAGAAGTT TTGCGAGACC CAGACCTTCT TCCCCAAGAA 
AGGAAAGCCG CCGCGAATAT CCTCAGGGCC CTTTCACAAG ATGACCAAGA TGACATCCAC 
CTAAAATTAG AGGATATAAT TCAAATGACT GACTGCATGA AGGCCGAATG CTTTGAGTCC 
TTGTCGGCCA TGGAGCTGGC AGAACAGATC ACCCTCCTGG ACCATGTCAT TTTCAGAAGC 
ATTCCCTACG AGGAGTTTCT TGGGCAGGGG TGGATGAAGC TGGATAAAAA CGAAAGAACT 
CCTTACATTA TGAAAACCAG CCAACACTTC AATGACATGA GTAACCTGGT GGCCTCCCAG 
ATAATGAACT ATGCTGATGT CAGCTCCCGT GCCAACGCCA TCGAGAAATG GGTGGCAGTG 
GCGGACATCT GCCGATGCCT GCACAACTAC AACGGCGTGC TGGAGATCAC CTCGGCCTTA 
AACAGAAGTG CCATCTACAG GCTGAAGAAA ACCTGGGCCA AGGTCTCTAA GCAGACAAAA 
GCTCTAATGG ACAAACTTCA AAAGACTGTT TCCTCTGAAG GAAGATTTAA AAATCTTAGA 
GAAACCCTTA AAAATTGTAA CCCTCCTGCA GTTCCTTATC TTGGGATGTA CTTGACAGAC 
CTGGCATTTA TTGAAGAAGG AACACCAAAC TTTACTGAGG AAGGCCTTGT CAATTTCTCC 
AAAATGAGAA TGATATCACA CATCATCAGA GAGATACGCC AGTTCCAGCA GACTTCCTAC 
AGAATAGATC ATCAGCCAAA GGTCGCACAG TACTTGCTTG ACAAAGACCT TATCATAGAT 
GAAGATACGC TATATGAGCT GTCACTAAAA ATTGAACCTC GACTCCCTGC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_008840.1
CDS377..4090
Translation

Target ORF information:

RefSeq Version NM_006909.3
Organism Homo sapiens(human)
Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006909.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGACAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGATTTCG AACTCAACAA TGAACTAAAG 
ATGAATGTCC TAAATTTGCT AGAAGAAGTT TTGCGAGACC CAGACCTTCT TCCCCAAGAA 
AGGAAAGCCG CCGCGAATAT CCTCAGGGCC CTTTCACAAG ATGACCAAGA TGACATCCAC 
CTAAAATTAG AGGATATAAT TCAAATGACT GACTGCATGA AGGCCGAATG CTTTGAGTCC 
TTGTCGGCCA TGGAGCTGGC AGAACAGATC ACCCTCCTGG ACCATGTCAT TTTCAGAAGC 
ATTCCCTACG AGGAGTTTCT TGGGCAGGGG TGGATGAAGC TGGATAAAAA CGAAAGAACT 
CCTTACATTA TGAAAACCAG CCAACACTTC AATGACATGA GTAACCTGGT GGCCTCCCAG 
ATAATGAACT ATGCTGATGT CAGCTCCCGT GCCAACGCCA TCGAGAAATG GGTGGCAGTG 
GCGGACATCT GCCGATGCCT GCACAACTAC AACGGCGTGC TGGAGATCAC CTCGGCCTTA 
AACAGAAGTG CCATCTACAG GCTGAAGAAA ACCTGGGCCA AGGTCTCTAA GCAGACAAAA 
GCTCTAATGG ACAAACTTCA AAAGACTGTT TCCTCTGAAG GAAGATTTAA AAATCTTAGA 
GAAACCCTTA AAAATTGTAA CCCTCCTGCA GTTCCTTATC TTGGGATGTA CTTGACAGAC 
CTGGCATTTA TTGAAGAAGG AACACCAAAC TTTACTGAGG AAGGCCTTGT CAATTTCTCC 
AAAATGAGAA TGATATCACA CATCATCAGA GAGATACGCC AGTTCCAGCA GACTTCCTAC 
AGAATAGATC ATCAGCCAAA GGTCGCACAG TACTTGCTTG ACAAAGACCT TATCATAGAT 
GAAGATACGC TATATGAGCT GTCACTAAAA ATTGAACCTC GACTCCCTGC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39662
Clone ID Related Accession (Same CDS sequence) XM_005248565.1
Accession Version XM_005248565.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGACAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGGAGGCC ACTTCGATTT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005248622.1
CDS68..2881
Translation

Target ORF information:

RefSeq Version XM_005248565.1
Organism Homo sapiens(human)
Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005248565.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGACAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGGCTT TAACAACACC 
GAGAGAACAT GTGATAAAGA GTTTATTATA CGGAGAACGG CTACCAATCG AGTTCTGAAC 
GTCCTCCGTC ACTGGGTCTC AAAGCACGCA CAGGGAGGCC ACTTCGATTT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu105682
Clone ID Related Accession (Same CDS sequence) XM_024446141.1
Accession Version XM_024446141.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CTCTTGAATT CATGGCCTGG TTGTTAAAAT TCATGATGTT TTCAAGCAGC 
AGTCCTAGAG TCTGCACCAG CGGACCGAGC AGGAGTGGAA AGCTCCCCTG CAGCGGACAC 
CACAGAACTT TCACCTTGCA GATCCCCCTC AACTCCTCGG CACCTCCGCT ATCGACAGCC 
TGGAGGACAG ACGGCGGACA ATGCCCACTG CTCTGTTTCA CCGGCTTCTG CTTTTGCAAT 
AGCCACAGCT GCAGCAGGAC ATGGGAGTCC ACCAGAGCCA TGAAGAGATC TTTCTCCGGT 
GTTTACTGGG AGGACTTGGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024301909.1
CDS68..2851
Translation

Target ORF information:

RefSeq Version XM_024446141.1
Organism Homo sapiens(human)
Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024446141.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CTCTTGAATT CATGGCCTGG TTGTTAAAAT TCATGATGTT TTCAAGCAGC 
AGTCCTAGAG TCTGCACCAG CGGACCGAGC AGGAGTGGAA AGCTCCCCTG CAGCGGACAC 
CACAGAACTT TCACCTTGCA GATCCCCCTC AACTCCTCGG CACCTCCGCT ATCGACAGCC 
TGGAGGACAG ACGGCGGACA ATGCCCACTG CTCTGTTTCA CCGGCTTCTG CTTTTGCAAT 
AGCCACAGCT GCAGCAGGAC ATGGGAGTCC ACCAGAGCCA TGAAGAGATC TTTCTCCGGT 
GTTTACTGGG AGGACTTGGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu105683
Clone ID Related Accession (Same CDS sequence) XM_024446142.1
Accession Version XM_024446142.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1839bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGCGCTC TAAACATCCT CTACAAGAGT GCCTTATGCT GGACTTTTAC 
TTATATACCC TCAGGATGGC TTCAATGGCA AATAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024301910.1
CDS68..1906
Translation

Target ORF information:

RefSeq Version XM_024446142.1
Organism Homo sapiens(human)
Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024446142.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGCGCTC TAAACATCCT CTACAAGAGT GCCTTATGCT GGACTTTTAC 
TTATATACCC TCAGGATGGC TTCAATGGCA AATAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95975
Clone ID Related Accession (Same CDS sequence) XM_017009683.1
Accession Version XM_017009683.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGACAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGAGCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016865172.1
CDS68..2761
Translation

Target ORF information:

RefSeq Version XM_017009683.1
Organism Homo sapiens(human)
Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017009683.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT TCTGGCGCGC 
AAGGAGGGCA CCAAGCGCGG CTTCCTGAGT AAGAAGACGG CCGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AACGAACGCC CGCGCCACCC 
AGGGCCGGCG CCGGGCAGGG AGGCGTCCGA GACGCGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCATGAAGG TCAGAAGCCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGACATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATTG CTCTTAATAA AACCAAAGAA CGAATGCGAC CTTACCAAAG CAACCAAGAA 
GACGAAGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGCG AGGATGGTTG 
TGCAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT 
ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTGGAGG CAGAGTCAGA GTACGTTCAC 
CAGCTCTACA TCCTGGTCAA TGGCTTTCTC CGGCCCCTGC GTATGGCCGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TTAACAGTGA AACAATCATG 
TTTCTTCATG AAATATTTCA TCAAGGACTA AAGGCAAGGA TAGCAAACTG GCCCACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT GCAAGTTCTC GCCAATTGTA AGCAAAACAG AGATTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAATCCA GCCTGTGAGG GGAGGATGCT GGAGACATTC 
TTGACCTATC CCATGTTTCA GATCCCCAGA TATATCATCA CACTCCATGA GCTCCTTGCT 
CACACACCCC ATGAGCATGT GGAAAGGAAA AGCCTGGAGT TTGCCAAATC AAAGCTAGAG 
GAACTATCCA GAGTAATGCA CGATGAAGTC AGCGACACTG AAAACATAAG GAAAAACCTT 
GCCATCGAAA GAATGATCGT GGAGGGCTGT GACATCTTGC TGGACACCAG CCAAACGTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GGGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CGTTGTCTTT GAAAAAGGAA GGAGAGAGAC AATGCTTCTT ATTTACAAAA 
CACTTTTTAA TATGTACAAG AAGTTCAGGA GGGAAGCTTC ATCTGCTCAA GACAGGTGGG 
GTTCTGTCTC TAATAGACTG CACATTGATT GAGGAGCCAG ATGCAAGCGA TGATGACTCT 
AAAGGTTCTG GGCAAGTGTT TGGGCACCTG GATTTTAAAA TAGTGGTGGA GCCTCCTGAC 
GCTGCCGCCT TCACTGTTGT CTTGTTAGCA CCCTCACGCC AGGAGAAAGC TGCCTGGATG 
AGTGACATCA GTCAGTGTGT GGACAATATA CGATGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAGAATT CCAAAGTCAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCATAAA 
GACGACACTG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGT 
TATGCCAGCG TGGAGCGCCT CTTGGAACGA CTGACAGACT TGCGGTTTCT TAGTATTGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGT ATTTTCACTA CTGCCGCTGT GGTGCTGGGG 
AAACTCTCCG ACATATACAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAATTG 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGT 
CTGTGTCGCA AATTCTCTTC CCCGCCACCA CTGGCTGTGT CCAGAACATC TTCCCCAGTG 
AGGGCCAGAA AGCTGTCTTT GACTTCTCCC TTGAACTCAA AGATAGGAGC ATTGGACCTG 
ACAACTTCCA GCAGTCCCAC CACCACCACC CAGAGTCCCG CTGCGTCTCC ACCACCACAC 
ACTGGTCAGA TACCACTGGA TCTCAGCAGA GGCCTCTCTT CTCCAGAGCA AAGCCCGGGA 
ACGGTAGAAG AGAATGTCGA TAACCCACGC GTGGATCTGT GTAACAAGCT AAAACGAAGT 
ATTCAAAAAG CAGTCCTAGA GTCTGCACCA GCGGACCGAG CAGGAGTGGA AAGCTCCCCT 
GCAGCGGACA CCACAGAACT TTCACCTTGC AGATCCCCCT CAACTCCTCG GCACCTCCGC 
TATCGACAGC CTGGAGGACA GACGGCGGAC AATGCCCACT GCTCTGTTTC ACCGGCTTCT 
GCTTTTGCAA TAGCCACAGC TGCAGCAGGA CATGGGAGTC CACCAGAGCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95974
Clone ID Related Accession (Same CDS sequence) XM_017009682.2 , XM_017009682.1
Accession Version XM_017009682.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009682.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTATTACT TTACTGTTCT TTTTGGCCAT GAAGGTCAGA AGCCACTGGA GCTGCGCTGT 
GAGGAGGAGC AGGATGGTAA AGAGTGGATG GAGGCCATTC ACCAAGCCAG TTATGCAGAC 
ATTTTGATTG AGAGGGAAGT ATTAATGCAG AAGTACATTC ATCTAGTTCA GATCGTAGAG 
ACAGAAAAAA TTGCAGCTAA CCAACTCCGA CATCAACTTG AAGATCAAGA CACAGAAATC 
GAAAGGCTTA AATCAGAGAT TATTGCTCTT AATAAAACCA AAGAACGAAT GCGACCTTAC 
CAAAGCAACC AAGAAGACGA AGATCCAGAC ATCAAGAAGA TTAAAAAGGT TCAGAGCTTC 
ATGCGAGGAT GGTTGTGCAG AAGGAAATGG AAGACCATCG TGCAGGATTA CATTTGTTCT 
CCTCATGCTG AAAGTATGAG GAAGAGAAAC CAGATTGTGT TCACCATGGT GGAGGCAGAG 
TCAGAGTACG TTCACCAGCT CTACATCCTG GTCAATGGCT TTCTCCGGCC CCTGCGTATG 
GCCGCCAGCT CCAAGAAGCC CCCCATCAGC CACGACGACG TCAGCAGTAT TTTTCTTAAC 
AGTGAAACAA TCATGTTTCT TCATGAAATA TTTCATCAAG GACTAAAGGC AAGGATAGCA 
AACTGGCCCA CTTTAATTTT AGCTGATCTG TTTGATATTT TGCTCCCCAT GCTGAACATT 
TATCAAGAAT TTGTGCGTAA TCACCAGTAC AGCCTGCAAG TTCTCGCCAA TTGTAAGCAA 
AACAGAGATT TTGACAAACT CTTAAAACAG TATGAAGCCA ATCCAGCCTG TGAGGGGAGG 
ATGCTGGAGA CATTCTTGAC CTATCCCATG TTTCAGATCC CCAGATATAT CATCACACTC 
CATGAGCTCC TTGCTCACAC ACCCCATGAG CATGTGGAAA GGAAAAGCCT GGAGTTTGCC 
AAATCAAAGC TAGAGGAACT ATCCAGAGTA ATGCACGATG AAGTCAGCGA CACTGAAAAC 
ATAAGGAAAA ACCTTGCCAT CGAAAGAATG ATCGTGGAGG GCTGTGACAT CTTGCTGGAC 
ACCAGCCAAA CGTTCATCCG CCAAGGTTCT CTTATTCAAG TACCTTCCGT TGAGAGGGGG 
AAACTTAGTA AAGTTCGCCT GGGTTCGTTG TCTTTGAAAA AGGAAGGAGA GAGACAATGC 
TTCTTATTTA CAAAACACTT TTTAATATGT ACAAGAAGTT CAGGAGGGAA GCTTCATCTG 
CTCAAGACAG GTGGGGTTCT GTCTCTAATA GACTGCACAT TGATTGAGGA GCCAGATGCA 
AGCGATGATG ACTCTAAAGG TTCTGGGCAA GTGTTTGGGC ACCTGGATTT TAAAATAGTG 
GTGGAGCCTC CTGACGCTGC CGCCTTCACT GTTGTCTTGT TAGCACCCTC ACGCCAGGAG 
AAAGCTGCCT GGATGAGTGA CATCAGTCAG TGTGTGGACA ATATACGATG TAATGGTTTA 
ATGACTATAG TGTTTGAAGA GAATTCCAAA GTCACTGTGC CACATATGAT TAAGTCTGAT 
GCCCGTCTTC ATAAAGACGA CACTGACATT TGCTTCAGTA AAACACTCAA CTCCTGCAAA 
GTGCCCCAGA TCCGTTATGC CAGCGTGGAG CGCCTCTTGG AACGACTGAC AGACTTGCGG 
TTTCTTAGTA TTGATTTCCT CAACACCTTT CTGCACACCT ATCGTATTTT CACTACTGCC 
GCTGTGGTGC TGGGGAAACT CTCCGACATA TACAAGAGGC CTTTCACCTC CATCCCTGTC 
AGGTCATTGG AATTGTTTTT TGCTACCAGC CAGAACAACA GAGGTGAACA TTTGGTGGAT 
GGCAAATCCC CACGTCTGTG TCGCAAATTC TCTTCCCCGC CACCACTGGC TGTGTCCAGA 
ACATCTTCCC CAGTGAGGGC CAGAAAGCTG TCTTTGACTT CTCCCTTGAA CTCAAAGATA 
GGAGCATTGG ACCTGACAAC TTCCAGCAGT CCCACCACCA CCACCCAGAG TCCCGCTGCG 
TCTCCACCAC CACACACTGG TCAGATACCA CTGGATCTCA GCAGAGGCCT CTCTTCTCCA 
GAGCAAAGCC CGGGAACGGT AGAAGAGAAT GTCGATAACC CACGCGTGGA TCTGTGTAAC 
AAGCTAAAAC GAAGTATTCA AAAAGCAGTC CTAGAGTCTG CACCAGCGGA CCGAGCAGGA 
GTGGAAAGCT CCCCTGCAGC GGACACCACA GAACTTTCAC CTTGCAGATC CCCCTCAACT 
CCTCGGCACC TCCGCTATCG ACAGCCTGGA GGACAGACGG CGGACAATGC CCACTGCTCT 
GTTTCACCGG CTTCTGCTTT TGCAATAGCC ACAGCTGCAG CAGGACATGG GAGTCCACCA 
GGCTTTAACA ACACCGAGAG AACATGTGAT AAAGAGTTTA TTATACGGAG AACGGCTACC 
AATCGAGTTC TGAACGTCCT CCGTCACTGG GTCTCAAAGC ACGCACAGGA TTTCGAACTC 
AACAATGAAC TAAAGATGAA TGTCCTAAAT TTGCTAGAAG AAGTTTTGCG AGACCCAGAC 
CTTCTTCCCC AAGAAAGGAA AGCCGCCGCG AATATCCTCA GGGCCCTTTC ACAAGATGAC 
CAAGATGACA TCCACCTAAA ATTAGAGGAT ATAATTCAAA TGACTGACTG CATGAAGGCC 
GAATGCTTTG AGTCCTTGTC GGCCATGGAG CTGGCAGAAC AGATCACCCT CCTGGACCAT 
GTCATTTTCA GAAGCATTCC CTACGAGGAG TTTCTTGGGC AGGGGTGGAT GAAGCTGGAT 
AAAAACGAAA GAACTCCTTA CATTATGAAA ACCAGCCAAC ACTTCAATGA CATGAGTAAC 
CTGGTGGCCT CCCAGATAAT GAACTATGCT GATGTCAGCT CCCGTGCCAA CGCCATCGAG 
AAATGGGTGG CAGTGGCGGA CATCTGCCGA TGCCTGCACA ACTACAACGG CGTGCTGGAG 
ATCACCTCGG CCTTAAACAG AAGTGCCATC TACAGGCTGA AGAAAACCTG GGCCAAGGTC 
TCTAAGCAGA CAAAAGCTCT AATGGACAAA CTTCAAAAGA CTGTTTCCTC TGAAGGAAGA 
TTTAAAAATC TTAGAGAAAC CCTTAAAAAT TGTAACCCTC CTGCAGTTCC TTATCTTGGG 
ATGTACTTGA CAGACCTGGC ATTTATTGAA GAAGGAACAC CAAACTTTAC TGAGGAAGGC 
CTTGTCAATT TCTCCAAAAT GAGAATGATA TCACACATCA TCAGAGAGAT ACGCCAGTTC 
CAGCAGACTT CCTACAGAAT AGATCATCAG CCAAAGGTCG CACAGTACTT GCTTGACAAA 
GACCTTATCA TAGATGAAGA TACGCTATAT GAGCTGTCAC TAAAAATTGA ACCTCGACTC 
CCTGCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016865171.1
CDS18..3446
Translation

Target ORF information:

RefSeq Version XM_017009682.2
Organism Homo sapiens(human)
Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017009682.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTATTACT TTACTGTTCT TTTTGGCCAT GAAGGTCAGA AGCCACTGGA GCTGCGCTGT 
GAGGAGGAGC AGGATGGTAA AGAGTGGATG GAGGCCATTC ACCAAGCCAG TTATGCAGAC 
ATTTTGATTG AGAGGGAAGT ATTAATGCAG AAGTACATTC ATCTAGTTCA GATCGTAGAG 
ACAGAAAAAA TTGCAGCTAA CCAACTCCGA CATCAACTTG AAGATCAAGA CACAGAAATC 
GAAAGGCTTA AATCAGAGAT TATTGCTCTT AATAAAACCA AAGAACGAAT GCGACCTTAC 
CAAAGCAACC AAGAAGACGA AGATCCAGAC ATCAAGAAGA TTAAAAAGGT TCAGAGCTTC 
ATGCGAGGAT GGTTGTGCAG AAGGAAATGG AAGACCATCG TGCAGGATTA CATTTGTTCT 
CCTCATGCTG AAAGTATGAG GAAGAGAAAC CAGATTGTGT TCACCATGGT GGAGGCAGAG 
TCAGAGTACG TTCACCAGCT CTACATCCTG GTCAATGGCT TTCTCCGGCC CCTGCGTATG 
GCCGCCAGCT CCAAGAAGCC CCCCATCAGC CACGACGACG TCAGCAGTAT TTTTCTTAAC 
AGTGAAACAA TCATGTTTCT TCATGAAATA TTTCATCAAG GACTAAAGGC AAGGATAGCA 
AACTGGCCCA CTTTAATTTT AGCTGATCTG TTTGATATTT TGCTCCCCAT GCTGAACATT 
TATCAAGAAT TTGTGCGTAA TCACCAGTAC AGCCTGCAAG TTCTCGCCAA TTGTAAGCAA 
AACAGAGATT TTGACAAACT CTTAAAACAG TATGAAGCCA ATCCAGCCTG TGAGGGGAGG 
ATGCTGGAGA CATTCTTGAC CTATCCCATG TTTCAGATCC CCAGATATAT CATCACACTC 
CATGAGCTCC TTGCTCACAC ACCCCATGAG CATGTGGAAA GGAAAAGCCT GGAGTTTGCC 
AAATCAAAGC TAGAGGAACT ATCCAGAGTA ATGCACGATG AAGTCAGCGA CACTGAAAAC 
ATAAGGAAAA ACCTTGCCAT CGAAAGAATG ATCGTGGAGG GCTGTGACAT CTTGCTGGAC 
ACCAGCCAAA CGTTCATCCG CCAAGGTTCT CTTATTCAAG TACCTTCCGT TGAGAGGGGG 
AAACTTAGTA AAGTTCGCCT GGGTTCGTTG TCTTTGAAAA AGGAAGGAGA GAGACAATGC 
TTCTTATTTA CAAAACACTT TTTAATATGT ACAAGAAGTT CAGGAGGGAA GCTTCATCTG 
CTCAAGACAG GTGGGGTTCT GTCTCTAATA GACTGCACAT TGATTGAGGA GCCAGATGCA 
AGCGATGATG ACTCTAAAGG TTCTGGGCAA GTGTTTGGGC ACCTGGATTT TAAAATAGTG 
GTGGAGCCTC CTGACGCTGC CGCCTTCACT GTTGTCTTGT TAGCACCCTC ACGCCAGGAG 
AAAGCTGCCT GGATGAGTGA CATCAGTCAG TGTGTGGACA ATATACGATG TAATGGTTTA 
ATGACTATAG TGTTTGAAGA GAATTCCAAA GTCACTGTGC CACATATGAT TAAGTCTGAT 
GCCCGTCTTC ATAAAGACGA CACTGACATT TGCTTCAGTA AAACACTCAA CTCCTGCAAA 
GTGCCCCAGA TCCGTTATGC CAGCGTGGAG CGCCTCTTGG AACGACTGAC AGACTTGCGG 
TTTCTTAGTA TTGATTTCCT CAACACCTTT CTGCACACCT ATCGTATTTT CACTACTGCC 
GCTGTGGTGC TGGGGAAACT CTCCGACATA TACAAGAGGC CTTTCACCTC CATCCCTGTC 
AGGTCATTGG AATTGTTTTT TGCTACCAGC CAGAACAACA GAGGTGAACA TTTGGTGGAT 
GGCAAATCCC CACGTCTGTG TCGCAAATTC TCTTCCCCGC CACCACTGGC TGTGTCCAGA 
ACATCTTCCC CAGTGAGGGC CAGAAAGCTG TCTTTGACTT CTCCCTTGAA CTCAAAGATA 
GGAGCATTGG ACCTGACAAC TTCCAGCAGT CCCACCACCA CCACCCAGAG TCCCGCTGCG 
TCTCCACCAC CACACACTGG TCAGATACCA CTGGATCTCA GCAGAGGCCT CTCTTCTCCA 
GAGCAAAGCC CGGGAACGGT AGAAGAGAAT GTCGATAACC CACGCGTGGA TCTGTGTAAC 
AAGCTAAAAC GAAGTATTCA AAAAGCAGTC CTAGAGTCTG CACCAGCGGA CCGAGCAGGA 
GTGGAAAGCT CCCCTGCAGC GGACACCACA GAACTTTCAC CTTGCAGATC CCCCTCAACT 
CCTCGGCACC TCCGCTATCG ACAGCCTGGA GGACAGACGG CGGACAATGC CCACTGCTCT 
GTTTCACCGG CTTCTGCTTT TGCAATAGCC ACAGCTGCAG CAGGACATGG GAGTCCACCA 
GGCTTTAACA ACACCGAGAG AACATGTGAT AAAGAGTTTA TTATACGGAG AACGGCTACC 
AATCGAGTTC TGAACGTCCT CCGTCACTGG GTCTCAAAGC ACGCACAGGA TTTCGAACTC 
AACAATGAAC TAAAGATGAA TGTCCTAAAT TTGCTAGAAG AAGTTTTGCG AGACCCAGAC 
CTTCTTCCCC AAGAAAGGAA AGCCGCCGCG AATATCCTCA GGGCCCTTTC ACAAGATGAC 
CAAGATGACA TCCACCTAAA ATTAGAGGAT ATAATTCAAA TGACTGACTG CATGAAGGCC 
GAATGCTTTG AGTCCTTGTC GGCCATGGAG CTGGCAGAAC AGATCACCCT CCTGGACCAT 
GTCATTTTCA GAAGCATTCC CTACGAGGAG TTTCTTGGGC AGGGGTGGAT GAAGCTGGAT 
AAAAACGAAA GAACTCCTTA CATTATGAAA ACCAGCCAAC ACTTCAATGA CATGAGTAAC 
CTGGTGGCCT CCCAGATAAT GAACTATGCT GATGTCAGCT CCCGTGCCAA CGCCATCGAG 
AAATGGGTGG CAGTGGCGGA CATCTGCCGA TGCCTGCACA ACTACAACGG CGTGCTGGAG 
ATCACCTCGG CCTTAAACAG AAGTGCCATC TACAGGCTGA AGAAAACCTG GGCCAAGGTC 
TCTAAGCAGA CAAAAGCTCT AATGGACAAA CTTCAAAAGA CTGTTTCCTC TGAAGGAAGA 
TTTAAAAATC TTAGAGAAAC CCTTAAAAAT TGTAACCCTC CTGCAGTTCC TTATCTTGGG 
ATGTACTTGA CAGACCTGGC ATTTATTGAA GAAGGAACAC CAAACTTTAC TGAGGAAGGC 
CTTGTCAATT TCTCCAAAAT GAGAATGATA TCACACATCA TCAGAGAGAT ACGCCAGTTC 
CAGCAGACTT CCTACAGAAT AGATCATCAG CCAAAGGTCG CACAGTACTT GCTTGACAAA 
GACCTTATCA TAGATGAAGA TACGCTATAT GAGCTGTCAC TAAAAATTGA ACCTCGACTC 
CCTGCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95974
Clone ID Related Accession (Same CDS sequence) XM_017009682.2 , XM_017009682.1
Accession Version XM_017009682.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTATTACT TTACTGTTCT TTTTGGCCAT GAAGGTCAGA AGCCACTGGA GCTGCGCTGT 
GAGGAGGAGC AGGATGGTAA AGAGTGGATG GAGGCCATTC ACCAAGCCAG TTATGCAGAC 
ATTTTGATTG AGAGGGAAGT ATTAATGCAG AAGTACATTC ATCTAGTTCA GATCGTAGAG 
ACAGAAAAAA TTGCAGCTAA CCAACTCCGA CATCAACTTG AAGATCAAGA CACAGAAATC 
GAAAGGCTTA AATCAGAGAT TATTGCTCTT AATAAAACCA AAGAACGAAT GCGACCTTAC 
CAAAGCAACC AAGAAGACGA AGATCCAGAC ATCAAGAAGA TTAAAAAGGT TCAGAGCTTC 
ATGCGAGGAT GGTTGTGCAG AAGGAAATGG AAGACCATCG TGCAGGATTA CATTTGTTCT 
CCTCATGCTG AAAGTATGAG GAAGAGAAAC CAGATTGTGT TCACCATGGT GGAGGCAGAG 
TCAGAGTACG TTCACCAGCT CTACATCCTG GTCAATGGCT TTCTCCGGCC CCTGCGTATG 
GCCGCCAGCT CCAAGAAGCC CCCCATCAGC CACGACGACG TCAGCAGTAT TTTTCTTAAC 
AGTGAAACAA TCATGTTTCT TCATGAAATA TTTCATCAAG GACTAAAGGC AAGGATAGCA 
AACTGGCCCA CTTTAATTTT AGCTGATCTG TTTGATATTT TGCTCCCCAT GCTGAACATT 
TATCAAGAAT TTGTGCGTAA TCACCAGTAC AGCCTGCAAG TTCTCGCCAA TTGTAAGCAA 
AACAGAGATT TTGACAAACT CTTAAAACAG TATGAAGCCA ATCCAGCCTG TGAGGGGAGG 
ATGCTGGAGA CATTCTTGAC CTATCCCATG TTTCAGATCC CCAGATATAT CATCACACTC 
CATGAGCTCC TTGCTCACAC ACCCCATGAG CATGTGGAAA GGAAAAGCCT GGAGTTTGCC 
AAATCAAAGC TAGAGGAACT ATCCAGAGTA ATGCACGATG AAGTCAGCGA CACTGAAAAC 
ATAAGGAAAA ACCTTGCCAT CGAAAGAATG ATCGTGGAGG GCTGTGACAT CTTGCTGGAC 
ACCAGCCAAA CGTTCATCCG CCAAGGTTCT CTTATTCAAG TACCTTCCGT TGAGAGGGGG 
AAACTTAGTA AAGTTCGCCT GGGTTCGTTG TCTTTGAAAA AGGAAGGAGA GAGACAATGC 
TTCTTATTTA CAAAACACTT TTTAATATGT ACAAGAAGTT CAGGAGGGAA GCTTCATCTG 
CTCAAGACAG GTGGGGTTCT GTCTCTAATA GACTGCACAT TGATTGAGGA GCCAGATGCA 
AGCGATGATG ACTCTAAAGG TTCTGGGCAA GTGTTTGGGC ACCTGGATTT TAAAATAGTG 
GTGGAGCCTC CTGACGCTGC CGCCTTCACT GTTGTCTTGT TAGCACCCTC ACGCCAGGAG 
AAAGCTGCCT GGATGAGTGA CATCAGTCAG TGTGTGGACA ATATACGATG TAATGGTTTA 
ATGACTATAG TGTTTGAAGA GAATTCCAAA GTCACTGTGC CACATATGAT TAAGTCTGAT 
GCCCGTCTTC ATAAAGACGA CACTGACATT TGCTTCAGTA AAACACTCAA CTCCTGCAAA 
GTGCCCCAGA TCCGTTATGC CAGCGTGGAG CGCCTCTTGG AACGACTGAC AGACTTGCGG 
TTTCTTAGTA TTGATTTCCT CAACACCTTT CTGCACACCT ATCGTATTTT CACTACTGCC 
GCTGTGGTGC TGGGGAAACT CTCCGACATA TACAAGAGGC CTTTCACCTC CATCCCTGTC 
AGGTCATTGG AATTGTTTTT TGCTACCAGC CAGAACAACA GAGGTGAACA TTTGGTGGAT 
GGCAAATCCC CACGTCTGTG TCGCAAATTC TCTTCCCCGC CACCACTGGC TGTGTCCAGA 
ACATCTTCCC CAGTGAGGGC CAGAAAGCTG TCTTTGACTT CTCCCTTGAA CTCAAAGATA 
GGAGCATTGG ACCTGACAAC TTCCAGCAGT CCCACCACCA CCACCCAGAG TCCCGCTGCG 
TCTCCACCAC CACACACTGG TCAGATACCA CTGGATCTCA GCAGAGGCCT CTCTTCTCCA 
GAGCAAAGCC CGGGAACGGT AGAAGAGAAT GTCGATAACC CACGCGTGGA TCTGTGTAAC 
AAGCTAAAAC GAAGTATTCA AAAAGCAGTC CTAGAGTCTG CACCAGCGGA CCGAGCAGGA 
GTGGAAAGCT CCCCTGCAGC GGACACCACA GAACTTTCAC CTTGCAGATC CCCCTCAACT 
CCTCGGCACC TCCGCTATCG ACAGCCTGGA GGACAGACGG CGGACAATGC CCACTGCTCT 
GTTTCACCGG CTTCTGCTTT TGCAATAGCC ACAGCTGCAG CAGGACATGG GAGTCCACCA 
GGCTTTAACA ACACCGAGAG AACATGTGAT AAAGAGTTTA TTATACGGAG AACGGCTACC 
AATCGAGTTC TGAACGTCCT CCGTCACTGG GTCTCAAAGC ACGCACAGGA TTTCGAACTC 
AACAATGAAC TAAAGATGAA TGTCCTAAAT TTGCTAGAAG AAGTTTTGCG AGACCCAGAC 
CTTCTTCCCC AAGAAAGGAA AGCCGCCGCG AATATCCTCA GGGCCCTTTC ACAAGATGAC 
CAAGATGACA TCCACCTAAA ATTAGAGGAT ATAATTCAAA TGACTGACTG CATGAAGGCC 
GAATGCTTTG AGTCCTTGTC GGCCATGGAG CTGGCAGAAC AGATCACCCT CCTGGACCAT 
GTCATTTTCA GAAGCATTCC CTACGAGGAG TTTCTTGGGC AGGGGTGGAT GAAGCTGGAT 
AAAAACGAAA GAACTCCTTA CATTATGAAA ACCAGCCAAC ACTTCAATGA CATGAGTAAC 
CTGGTGGCCT CCCAGATAAT GAACTATGCT GATGTCAGCT CCCGTGCCAA CGCCATCGAG 
AAATGGGTGG CAGTGGCGGA CATCTGCCGA TGCCTGCACA ACTACAACGG CGTGCTGGAG 
ATCACCTCGG CCTTAAACAG AAGTGCCATC TACAGGCTGA AGAAAACCTG GGCCAAGGTC 
TCTAAGCAGA CAAAAGCTCT AATGGACAAA CTTCAAAAGA CTGTTTCCTC TGAAGGAAGA 
TTTAAAAATC TTAGAGAAAC CCTTAAAAAT TGTAACCCTC CTGCAGTTCC TTATCTTGGG 
ATGTACTTGA CAGACCTGGC ATTTATTGAA GAAGGAACAC CAAACTTTAC TGAGGAAGGC 
CTTGTCAATT TCTCCAAAAT GAGAATGATA TCACACATCA TCAGAGAGAT ACGCCAGTTC 
CAGCAGACTT CCTACAGAAT AGATCATCAG CCAAAGGTCG CACAGTACTT GCTTGACAAA 
GACCTTATCA TAGATGAAGA TACGCTATAT GAGCTGTCAC TAAAAATTGA ACCTCGACTC 
CCTGCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016865171.1
CDS18..3446
Translation

Target ORF information:

RefSeq Version XM_017009682.1
Organism Homo sapiens(human)
Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017009682.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTATTACT TTACTGTTCT TTTTGGCCAT GAAGGTCAGA AGCCACTGGA GCTGCGCTGT 
GAGGAGGAGC AGGATGGTAA AGAGTGGATG GAGGCCATTC ACCAAGCCAG TTATGCAGAC 
ATTTTGATTG AGAGGGAAGT ATTAATGCAG AAGTACATTC ATCTAGTTCA GATCGTAGAG 
ACAGAAAAAA TTGCAGCTAA CCAACTCCGA CATCAACTTG AAGATCAAGA CACAGAAATC 
GAAAGGCTTA AATCAGAGAT TATTGCTCTT AATAAAACCA AAGAACGAAT GCGACCTTAC 
CAAAGCAACC AAGAAGACGA AGATCCAGAC ATCAAGAAGA TTAAAAAGGT TCAGAGCTTC 
ATGCGAGGAT GGTTGTGCAG AAGGAAATGG AAGACCATCG TGCAGGATTA CATTTGTTCT 
CCTCATGCTG AAAGTATGAG GAAGAGAAAC CAGATTGTGT TCACCATGGT GGAGGCAGAG 
TCAGAGTACG TTCACCAGCT CTACATCCTG GTCAATGGCT TTCTCCGGCC CCTGCGTATG 
GCCGCCAGCT CCAAGAAGCC CCCCATCAGC CACGACGACG TCAGCAGTAT TTTTCTTAAC 
AGTGAAACAA TCATGTTTCT TCATGAAATA TTTCATCAAG GACTAAAGGC AAGGATAGCA 
AACTGGCCCA CTTTAATTTT AGCTGATCTG TTTGATATTT TGCTCCCCAT GCTGAACATT 
TATCAAGAAT TTGTGCGTAA TCACCAGTAC AGCCTGCAAG TTCTCGCCAA TTGTAAGCAA 
AACAGAGATT TTGACAAACT CTTAAAACAG TATGAAGCCA ATCCAGCCTG TGAGGGGAGG 
ATGCTGGAGA CATTCTTGAC CTATCCCATG TTTCAGATCC CCAGATATAT CATCACACTC 
CATGAGCTCC TTGCTCACAC ACCCCATGAG CATGTGGAAA GGAAAAGCCT GGAGTTTGCC 
AAATCAAAGC TAGAGGAACT ATCCAGAGTA ATGCACGATG AAGTCAGCGA CACTGAAAAC 
ATAAGGAAAA ACCTTGCCAT CGAAAGAATG ATCGTGGAGG GCTGTGACAT CTTGCTGGAC 
ACCAGCCAAA CGTTCATCCG CCAAGGTTCT CTTATTCAAG TACCTTCCGT TGAGAGGGGG 
AAACTTAGTA AAGTTCGCCT GGGTTCGTTG TCTTTGAAAA AGGAAGGAGA GAGACAATGC 
TTCTTATTTA CAAAACACTT TTTAATATGT ACAAGAAGTT CAGGAGGGAA GCTTCATCTG 
CTCAAGACAG GTGGGGTTCT GTCTCTAATA GACTGCACAT TGATTGAGGA GCCAGATGCA 
AGCGATGATG ACTCTAAAGG TTCTGGGCAA GTGTTTGGGC ACCTGGATTT TAAAATAGTG 
GTGGAGCCTC CTGACGCTGC CGCCTTCACT GTTGTCTTGT TAGCACCCTC ACGCCAGGAG 
AAAGCTGCCT GGATGAGTGA CATCAGTCAG TGTGTGGACA ATATACGATG TAATGGTTTA 
ATGACTATAG TGTTTGAAGA GAATTCCAAA GTCACTGTGC CACATATGAT TAAGTCTGAT 
GCCCGTCTTC ATAAAGACGA CACTGACATT TGCTTCAGTA AAACACTCAA CTCCTGCAAA 
GTGCCCCAGA TCCGTTATGC CAGCGTGGAG CGCCTCTTGG AACGACTGAC AGACTTGCGG 
TTTCTTAGTA TTGATTTCCT CAACACCTTT CTGCACACCT ATCGTATTTT CACTACTGCC 
GCTGTGGTGC TGGGGAAACT CTCCGACATA TACAAGAGGC CTTTCACCTC CATCCCTGTC 
AGGTCATTGG AATTGTTTTT TGCTACCAGC CAGAACAACA GAGGTGAACA TTTGGTGGAT 
GGCAAATCCC CACGTCTGTG TCGCAAATTC TCTTCCCCGC CACCACTGGC TGTGTCCAGA 
ACATCTTCCC CAGTGAGGGC CAGAAAGCTG TCTTTGACTT CTCCCTTGAA CTCAAAGATA 
GGAGCATTGG ACCTGACAAC TTCCAGCAGT CCCACCACCA CCACCCAGAG TCCCGCTGCG 
TCTCCACCAC CACACACTGG TCAGATACCA CTGGATCTCA GCAGAGGCCT CTCTTCTCCA 
GAGCAAAGCC CGGGAACGGT AGAAGAGAAT GTCGATAACC CACGCGTGGA TCTGTGTAAC 
AAGCTAAAAC GAAGTATTCA AAAAGCAGTC CTAGAGTCTG CACCAGCGGA CCGAGCAGGA 
GTGGAAAGCT CCCCTGCAGC GGACACCACA GAACTTTCAC CTTGCAGATC CCCCTCAACT 
CCTCGGCACC TCCGCTATCG ACAGCCTGGA GGACAGACGG CGGACAATGC CCACTGCTCT 
GTTTCACCGG CTTCTGCTTT TGCAATAGCC ACAGCTGCAG CAGGACATGG GAGTCCACCA 
GGCTTTAACA ACACCGAGAG AACATGTGAT AAAGAGTTTA TTATACGGAG AACGGCTACC 
AATCGAGTTC TGAACGTCCT CCGTCACTGG GTCTCAAAGC ACGCACAGGA TTTCGAACTC 
AACAATGAAC TAAAGATGAA TGTCCTAAAT TTGCTAGAAG AAGTTTTGCG AGACCCAGAC 
CTTCTTCCCC AAGAAAGGAA AGCCGCCGCG AATATCCTCA GGGCCCTTTC ACAAGATGAC 
CAAGATGACA TCCACCTAAA ATTAGAGGAT ATAATTCAAA TGACTGACTG CATGAAGGCC 
GAATGCTTTG AGTCCTTGTC GGCCATGGAG CTGGCAGAAC AGATCACCCT CCTGGACCAT 
GTCATTTTCA GAAGCATTCC CTACGAGGAG TTTCTTGGGC AGGGGTGGAT GAAGCTGGAT 
AAAAACGAAA GAACTCCTTA CATTATGAAA ACCAGCCAAC ACTTCAATGA CATGAGTAAC 
CTGGTGGCCT CCCAGATAAT GAACTATGCT GATGTCAGCT CCCGTGCCAA CGCCATCGAG 
AAATGGGTGG CAGTGGCGGA CATCTGCCGA TGCCTGCACA ACTACAACGG CGTGCTGGAG 
ATCACCTCGG CCTTAAACAG AAGTGCCATC TACAGGCTGA AGAAAACCTG GGCCAAGGTC 
TCTAAGCAGA CAAAAGCTCT AATGGACAAA CTTCAAAAGA CTGTTTCCTC TGAAGGAAGA 
TTTAAAAATC TTAGAGAAAC CCTTAAAAAT TGTAACCCTC CTGCAGTTCC TTATCTTGGG 
ATGTACTTGA CAGACCTGGC ATTTATTGAA GAAGGAACAC CAAACTTTAC TGAGGAAGGC 
CTTGTCAATT TCTCCAAAAT GAGAATGATA TCACACATCA TCAGAGAGAT ACGCCAGTTC 
CAGCAGACTT CCTACAGAAT AGATCATCAG CCAAAGGTCG CACAGTACTT GCTTGACAAA 
GACCTTATCA TAGATGAAGA TACGCTATAT GAGCTGTCAC TAAAAATTGA ACCTCGACTC 
CCTGCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.
Arteriosclerosis, thrombosis, and vascular biology37(1)98-107(2017 Jan)
Wu RF,Liao C,Hatoum H,Fu G,Ochoa CD,Terada LS


book

A Single Nucleotide Polymorphism in the RASGRF2 Gene Is Associated with Alcoholic Liver Cirrhosis in Men.
PloS one11(12)e0168685(2016)
Novo-Veleiro I,Cieza-Borrella C,Pastor I,Chamorro AJ,Laso FJ,Gonz?lez-Sarmiento R,Marcos M


book

Common variants at 1p36 are associated with superior frontal gyrus volume.
Translational psychiatry4e472(2014 Oct)
Hashimoto R,Ikeda M,Yamashita F,Ohi K,Yamamori H,Yasuda Y,Fujimoto M,Fukunaga M,Nemoto K,Takahashi T,Tochigi M,Onitsuka T,Yamasue H,Matsuo K,Iidaka T,Iwata N,Suzuki M,Takeda M,Kasai K,Ozaki N


book

?Arrestin1 regulates the guanine nucleotide exchange factor RasGRF2 expression and the small GTPase Rac-mediated formation of membrane protrusion and cell motility.
The Journal of biological chemistry289(19)13638-50(2014 May)
Ma X,Espana-Serrano L,Kim WJ,Thayele Purayil H,Nie Z,Daaka Y