• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

RASGRF2 cDNA ORF clone, Gallus gallus(chicken)

Summary

Gene Symbol RASGRF2
Entrez Gene ID 100859132
Full Name Ras protein specific guanine nucleotide releasing factor 2
General protein information
Preferred Names

Ras protein specific guanine nucleotide releasing factor 2

Names

ras-specific guanine nucleotide-releasing factor 2
Gene Type protein-coding
Organism Gallus gallus(chicken)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015280709.1 XP_015136195.1 ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_003643077.3 XP_003643125.1 ras-specific guanine nucleotide-releasing factor 2 isoform X4
XM_015280709.2 XP_015136195.1 ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_004949460.3 XP_004949517.1 ras-specific guanine nucleotide-releasing factor 2 isoform X3
XM_004949460.2 XP_004949517.1 ras-specific guanine nucleotide-releasing factor 2 isoform X3
XM_015280710.2 XP_015136196.1 ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_003643077.4 XP_003643125.1 ras-specific guanine nucleotide-releasing factor 2 isoform X4
XM_015280710.1 XP_015136196.1 ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_015280711.2 XP_015136197.1 ras-specific guanine nucleotide-releasing factor 2 isoform X5
XM_015280711.1 XP_015136197.1 ras-specific guanine nucleotide-releasing factor 2 isoform X5

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) RASGRF2 XP_005618219.1
Danio rerio (zebrafish) rasgrf2b NP_001121705.1
Pan troglodytes (chimpanzee) RASGRF2 XP_517672.2
Xenopus tropicalis (tropical clawed frog) rasgrf2 XP_002942408.2
Homo sapiens (human) RASGRF2 NP_008840.1
Mus musculus (house mouse) Rasgrf2 NP_033053.2
Rattus norvegicus (Norway rat) Rasgrf2 NP_446173.1
Gallus gallus (chicken) RASGRF2 XP_004949517.1
Bos taurus (cattle) RASGRF2 XP_002689419.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001238 XP_321917.5

Gene Ontology

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa21740 XM_004949460.3
Latest version!		 Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OGa21741 XM_003643077.4
Latest version!		 Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OGa48314 XM_015280709.2
Latest version!		 Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OGa48315 XM_015280710.2
Latest version!		 Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OGa48316 XM_015280711.2
Latest version!		 Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OGa48314 XM_015280709.1 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OGa21740 XM_004949460.2 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OGa21741 XM_003643077.3 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OGa48315 XM_015280710.1 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OGa48316 XM_015280711.1 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OGa21740
Clone ID Related Accession (Same CDS sequence) XM_004949460.3 , XM_004949460.2
Accession Version XM_004949460.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004949460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC 
ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA 
CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT 
CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA 
AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT 
CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT 
GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC 
CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA 
CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT 
TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC 
TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA 
ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA 
ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA 
AGCATTCGAA GAGCAGCAGT TCTGGAATCA GTGTCAGTGG ACAGAACTAT TCCTGAAAGC 
ACCTCGGCAG TGGATACCAC AGAACTTTCC CCGTGTAGAT CCCCTTCAAC ACCACGTCAT 
CTCCGCTATC GTCAATCAGG AGTGCAAACT GCTGACAACA GTCACTGTGC TGTTTCTCCT 
GCATCAGCTT TTGCTATTGC TACAGCTGCA GCAGGGCATG GGAGTCCGCC AGGTTTTAAC 
AATTCTGAAC GAATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC TAATCGTGTC 
CTGAATGTCT TACGTCACTG GGTCTCCAAG CACTCTCAGG ATTTTGAACT AAACAATGAG 
CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCTGA CCTTCTACCA 
CAAGAAAGGA AAGCTACTGC AAATATACTA AGGGCACTTT CTCAGGACGA TCAAGATGAT 
ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGAAT GTCCAAAACC GGAGTGCTTT 
GAGACTTTGT CTGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGACCA CATAGTTTTC 
AGAAGCATAC CCTATGAAGA GTTTCTTGGG CAGGGCTGGA TGAAACTGGA TAAAAATGAG 
AGAACACCCT ATATTATGAA AACTAGTCAA CATTTTAATG ATATGAGTAA CCTTGTTGCC 
TCCCAAATAA TGAATTATGC TGATGTCAGC TCCCGGGCTA ACTCAATTGA AAAGTGGGTA 
GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTACAATG GTGTGTTAGA AATCACGTCA 
GCCTTGAACA GAAGTGCAAT CTACAGACTG AAGAAAACTT GGGCTAAGGT ATCCAAACAG 
GCAAAAGCAC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAT 
CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CATACCTTGG AATGTATTTG 
ACAGATCTAG CATTTATTGA AGAAGGAACA CCAAACTTCA CTGAGGAAGG CCTTGTTAAT 
TTTTCCAAGA TGAGAATGAT CTCACACATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC 
TCCTACCGCA TAGAACATCA GCAGAAGGTC ACTCACTACT TACTCGACAA GGCACTCATC 
ATAGATGAAG ATACTTTGTA TGAGCTTTCC CTGAAGCTTG AACCCAGACT CCCCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_004949517.1
CDS293..4012
Translation

Target ORF information:

RefSeq Version XM_004949460.3
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_004949460.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC 
ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA 
CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT 
CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA 
AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT 
CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT 
GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC 
CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA 
CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT 
TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC 
TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA 
ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA 
ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA 
AGCATTCGAA GAGCAGCAGT TCTGGAATCA GTGTCAGTGG ACAGAACTAT TCCTGAAAGC 
ACCTCGGCAG TGGATACCAC AGAACTTTCC CCGTGTAGAT CCCCTTCAAC ACCACGTCAT 
CTCCGCTATC GTCAATCAGG AGTGCAAACT GCTGACAACA GTCACTGTGC TGTTTCTCCT 
GCATCAGCTT TTGCTATTGC TACAGCTGCA GCAGGGCATG GGAGTCCGCC AGGTTTTAAC 
AATTCTGAAC GAATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC TAATCGTGTC 
CTGAATGTCT TACGTCACTG GGTCTCCAAG CACTCTCAGG ATTTTGAACT AAACAATGAG 
CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCTGA CCTTCTACCA 
CAAGAAAGGA AAGCTACTGC AAATATACTA AGGGCACTTT CTCAGGACGA TCAAGATGAT 
ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGAAT GTCCAAAACC GGAGTGCTTT 
GAGACTTTGT CTGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGACCA CATAGTTTTC 
AGAAGCATAC CCTATGAAGA GTTTCTTGGG CAGGGCTGGA TGAAACTGGA TAAAAATGAG 
AGAACACCCT ATATTATGAA AACTAGTCAA CATTTTAATG ATATGAGTAA CCTTGTTGCC 
TCCCAAATAA TGAATTATGC TGATGTCAGC TCCCGGGCTA ACTCAATTGA AAAGTGGGTA 
GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTACAATG GTGTGTTAGA AATCACGTCA 
GCCTTGAACA GAAGTGCAAT CTACAGACTG AAGAAAACTT GGGCTAAGGT ATCCAAACAG 
GCAAAAGCAC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAT 
CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CATACCTTGG AATGTATTTG 
ACAGATCTAG CATTTATTGA AGAAGGAACA CCAAACTTCA CTGAGGAAGG CCTTGTTAAT 
TTTTCCAAGA TGAGAATGAT CTCACACATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC 
TCCTACCGCA TAGAACATCA GCAGAAGGTC ACTCACTACT TACTCGACAA GGCACTCATC 
ATAGATGAAG ATACTTTGTA TGAGCTTTCC CTGAAGCTTG AACCCAGACT CCCCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa21740
Clone ID Related Accession (Same CDS sequence) XM_004949460.3 , XM_004949460.2
Accession Version XM_004949460.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004949460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC 
ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA 
CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT 
CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA 
AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT 
CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT 
GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC 
CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA 
CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT 
TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC 
TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA 
ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA 
ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA 
AGCATTCGAA GAGCAGCAGT TCTGGAATCA GTGTCAGTGG ACAGAACTAT TCCTGAAAGC 
ACCTCGGCAG TGGATACCAC AGAACTTTCC CCGTGTAGAT CCCCTTCAAC ACCACGTCAT 
CTCCGCTATC GTCAATCAGG AGTGCAAACT GCTGACAACA GTCACTGTGC TGTTTCTCCT 
GCATCAGCTT TTGCTATTGC TACAGCTGCA GCAGGGCATG GGAGTCCGCC AGGTTTTAAC 
AATTCTGAAC GAATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC TAATCGTGTC 
CTGAATGTCT TACGTCACTG GGTCTCCAAG CACTCTCAGG ATTTTGAACT AAACAATGAG 
CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCTGA CCTTCTACCA 
CAAGAAAGGA AAGCTACTGC AAATATACTA AGGGCACTTT CTCAGGACGA TCAAGATGAT 
ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGAAT GTCCAAAACC GGAGTGCTTT 
GAGACTTTGT CTGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGACCA CATAGTTTTC 
AGAAGCATAC CCTATGAAGA GTTTCTTGGG CAGGGCTGGA TGAAACTGGA TAAAAATGAG 
AGAACACCCT ATATTATGAA AACTAGTCAA CATTTTAATG ATATGAGTAA CCTTGTTGCC 
TCCCAAATAA TGAATTATGC TGATGTCAGC TCCCGGGCTA ACTCAATTGA AAAGTGGGTA 
GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTACAATG GTGTGTTAGA AATCACGTCA 
GCCTTGAACA GAAGTGCAAT CTACAGACTG AAGAAAACTT GGGCTAAGGT ATCCAAACAG 
GCAAAAGCAC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAT 
CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CATACCTTGG AATGTATTTG 
ACAGATCTAG CATTTATTGA AGAAGGAACA CCAAACTTCA CTGAGGAAGG CCTTGTTAAT 
TTTTCCAAGA TGAGAATGAT CTCACACATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC 
TCCTACCGCA TAGAACATCA GCAGAAGGTC ACTCACTACT TACTCGACAA GGCACTCATC 
ATAGATGAAG ATACTTTGTA TGAGCTTTCC CTGAAGCTTG AACCCAGACT CCCCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_004949517.1
CDS301..4020
Translation

Target ORF information:

RefSeq Version XM_004949460.2
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_004949460.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC 
ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA 
CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT 
CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA 
AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT 
CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT 
GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC 
CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA 
CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT 
TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC 
TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA 
ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA 
ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA 
AGCATTCGAA GAGCAGCAGT TCTGGAATCA GTGTCAGTGG ACAGAACTAT TCCTGAAAGC 
ACCTCGGCAG TGGATACCAC AGAACTTTCC CCGTGTAGAT CCCCTTCAAC ACCACGTCAT 
CTCCGCTATC GTCAATCAGG AGTGCAAACT GCTGACAACA GTCACTGTGC TGTTTCTCCT 
GCATCAGCTT TTGCTATTGC TACAGCTGCA GCAGGGCATG GGAGTCCGCC AGGTTTTAAC 
AATTCTGAAC GAATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC TAATCGTGTC 
CTGAATGTCT TACGTCACTG GGTCTCCAAG CACTCTCAGG ATTTTGAACT AAACAATGAG 
CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCTGA CCTTCTACCA 
CAAGAAAGGA AAGCTACTGC AAATATACTA AGGGCACTTT CTCAGGACGA TCAAGATGAT 
ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGAAT GTCCAAAACC GGAGTGCTTT 
GAGACTTTGT CTGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGACCA CATAGTTTTC 
AGAAGCATAC CCTATGAAGA GTTTCTTGGG CAGGGCTGGA TGAAACTGGA TAAAAATGAG 
AGAACACCCT ATATTATGAA AACTAGTCAA CATTTTAATG ATATGAGTAA CCTTGTTGCC 
TCCCAAATAA TGAATTATGC TGATGTCAGC TCCCGGGCTA ACTCAATTGA AAAGTGGGTA 
GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTACAATG GTGTGTTAGA AATCACGTCA 
GCCTTGAACA GAAGTGCAAT CTACAGACTG AAGAAAACTT GGGCTAAGGT ATCCAAACAG 
GCAAAAGCAC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAT 
CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CATACCTTGG AATGTATTTG 
ACAGATCTAG CATTTATTGA AGAAGGAACA CCAAACTTCA CTGAGGAAGG CCTTGTTAAT 
TTTTCCAAGA TGAGAATGAT CTCACACATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC 
TCCTACCGCA TAGAACATCA GCAGAAGGTC ACTCACTACT TACTCGACAA GGCACTCATC 
ATAGATGAAG ATACTTTGTA TGAGCTTTCC CTGAAGCTTG AACCCAGACT CCCCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa21741
Clone ID Related Accession (Same CDS sequence) XM_003643077.3 , XM_003643077.4
Accession Version XM_003643077.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003643077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC 
ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA 
CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT 
CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA 
AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT 
CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT 
GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC 
CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA 
CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT 
TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC 
TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA 
ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA 
ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA 
AGCATTCGAA GAGCAGTTCT GGAATCAGTG TCAGTGGACA GAACTATTCC TGAAAGCACC 
TCGGCAGTGG ATACCACAGA ACTTTCCCCG TGTAGATCCC CTTCAACACC ACGTCATCTC 
CGCTATCGTC AATCAGGAGT GCAAACTGCT GACAACAGTC ACTGTGCTGT TTCTCCTGCA 
TCAGCTTTTG CTATTGCTAC AGCTGCAGCA GGGCATGGGA GTCCGCCAGG TTTTAACAAT 
TCTGAACGAA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACTAA TCGTGTCCTG 
AATGTCTTAC GTCACTGGGT CTCCAAGCAC TCTCAGGATT TTGAACTAAA CAATGAGCTG 
AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCTGACCT TCTACCACAA 
GAAAGGAAAG CTACTGCAAA TATACTAAGG GCACTTTCTC AGGACGATCA AGATGATACC 
CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGAATGTC CAAAACCGGA GTGCTTTGAG 
ACTTTGTCTG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGACCACAT AGTTTTCAGA 
AGCATACCCT ATGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAGAGA 
ACACCCTATA TTATGAAAAC TAGTCAACAT TTTAATGATA TGAGTAACCT TGTTGCCTCC 
CAAATAATGA ATTATGCTGA TGTCAGCTCC CGGGCTAACT CAATTGAAAA GTGGGTAGCA 
GTAGCTGATA TTTGTCGATG TATGCACAAT TACAATGGTG TGTTAGAAAT CACGTCAGCC 
TTGAACAGAA GTGCAATCTA CAGACTGAAG AAAACTTGGG CTAAGGTATC CAAACAGGCA 
AAAGCACTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAATCTT 
AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCAT ACCTTGGAAT GTATTTGACA 
GATCTAGCAT TTATTGAAGA AGGAACACCA AACTTCACTG AGGAAGGCCT TGTTAATTTT 
TCCAAGATGA GAATGATCTC ACACATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC 
TACCGCATAG AACATCAGCA GAAGGTCACT CACTACTTAC TCGACAAGGC ACTCATCATA 
GATGAAGATA CTTTGTATGA GCTTTCCCTG AAGCTTGAAC CCAGACTCCC CGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003643125.1
CDS302..4018
Translation

Target ORF information:

RefSeq Version XM_003643077.3
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003643077.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC 
ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA 
CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT 
CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA 
AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT 
CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT 
GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC 
CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA 
CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT 
TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC 
TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA 
ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA 
ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA 
AGCATTCGAA GAGCAGTTCT GGAATCAGTG TCAGTGGACA GAACTATTCC TGAAAGCACC 
TCGGCAGTGG ATACCACAGA ACTTTCCCCG TGTAGATCCC CTTCAACACC ACGTCATCTC 
CGCTATCGTC AATCAGGAGT GCAAACTGCT GACAACAGTC ACTGTGCTGT TTCTCCTGCA 
TCAGCTTTTG CTATTGCTAC AGCTGCAGCA GGGCATGGGA GTCCGCCAGG TTTTAACAAT 
TCTGAACGAA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACTAA TCGTGTCCTG 
AATGTCTTAC GTCACTGGGT CTCCAAGCAC TCTCAGGATT TTGAACTAAA CAATGAGCTG 
AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCTGACCT TCTACCACAA 
GAAAGGAAAG CTACTGCAAA TATACTAAGG GCACTTTCTC AGGACGATCA AGATGATACC 
CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGAATGTC CAAAACCGGA GTGCTTTGAG 
ACTTTGTCTG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGACCACAT AGTTTTCAGA 
AGCATACCCT ATGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAGAGA 
ACACCCTATA TTATGAAAAC TAGTCAACAT TTTAATGATA TGAGTAACCT TGTTGCCTCC 
CAAATAATGA ATTATGCTGA TGTCAGCTCC CGGGCTAACT CAATTGAAAA GTGGGTAGCA 
GTAGCTGATA TTTGTCGATG TATGCACAAT TACAATGGTG TGTTAGAAAT CACGTCAGCC 
TTGAACAGAA GTGCAATCTA CAGACTGAAG AAAACTTGGG CTAAGGTATC CAAACAGGCA 
AAAGCACTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAATCTT 
AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCAT ACCTTGGAAT GTATTTGACA 
GATCTAGCAT TTATTGAAGA AGGAACACCA AACTTCACTG AGGAAGGCCT TGTTAATTTT 
TCCAAGATGA GAATGATCTC ACACATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC 
TACCGCATAG AACATCAGCA GAAGGTCACT CACTACTTAC TCGACAAGGC ACTCATCATA 
GATGAAGATA CTTTGTATGA GCTTTCCCTG AAGCTTGAAC CCAGACTCCC CGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa21741
Clone ID Related Accession (Same CDS sequence) XM_003643077.3 , XM_003643077.4
Accession Version XM_003643077.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003643077.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC 
ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA 
CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT 
CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA 
AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT 
CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT 
GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC 
CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA 
CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT 
TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC 
TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA 
ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA 
ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA 
AGCATTCGAA GAGCAGTTCT GGAATCAGTG TCAGTGGACA GAACTATTCC TGAAAGCACC 
TCGGCAGTGG ATACCACAGA ACTTTCCCCG TGTAGATCCC CTTCAACACC ACGTCATCTC 
CGCTATCGTC AATCAGGAGT GCAAACTGCT GACAACAGTC ACTGTGCTGT TTCTCCTGCA 
TCAGCTTTTG CTATTGCTAC AGCTGCAGCA GGGCATGGGA GTCCGCCAGG TTTTAACAAT 
TCTGAACGAA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACTAA TCGTGTCCTG 
AATGTCTTAC GTCACTGGGT CTCCAAGCAC TCTCAGGATT TTGAACTAAA CAATGAGCTG 
AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCTGACCT TCTACCACAA 
GAAAGGAAAG CTACTGCAAA TATACTAAGG GCACTTTCTC AGGACGATCA AGATGATACC 
CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGAATGTC CAAAACCGGA GTGCTTTGAG 
ACTTTGTCTG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGACCACAT AGTTTTCAGA 
AGCATACCCT ATGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAGAGA 
ACACCCTATA TTATGAAAAC TAGTCAACAT TTTAATGATA TGAGTAACCT TGTTGCCTCC 
CAAATAATGA ATTATGCTGA TGTCAGCTCC CGGGCTAACT CAATTGAAAA GTGGGTAGCA 
GTAGCTGATA TTTGTCGATG TATGCACAAT TACAATGGTG TGTTAGAAAT CACGTCAGCC 
TTGAACAGAA GTGCAATCTA CAGACTGAAG AAAACTTGGG CTAAGGTATC CAAACAGGCA 
AAAGCACTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAATCTT 
AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCAT ACCTTGGAAT GTATTTGACA 
GATCTAGCAT TTATTGAAGA AGGAACACCA AACTTCACTG AGGAAGGCCT TGTTAATTTT 
TCCAAGATGA GAATGATCTC ACACATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC 
TACCGCATAG AACATCAGCA GAAGGTCACT CACTACTTAC TCGACAAGGC ACTCATCATA 
GATGAAGATA CTTTGTATGA GCTTTCCCTG AAGCTTGAAC CCAGACTCCC CGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003643125.1
CDS293..4009
Translation

Target ORF information:

RefSeq Version XM_003643077.4
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003643077.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC 
ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA 
CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC 
ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA 
CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT 
TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT 
CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA 
AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT 
CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT 
GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA 
GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT 
GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT 
GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC 
CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA 
CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT 
TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC 
TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA 
ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT 
ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA 
ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT 
GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA 
AGCATTCGAA GAGCAGTTCT GGAATCAGTG TCAGTGGACA GAACTATTCC TGAAAGCACC 
TCGGCAGTGG ATACCACAGA ACTTTCCCCG TGTAGATCCC CTTCAACACC ACGTCATCTC 
CGCTATCGTC AATCAGGAGT GCAAACTGCT GACAACAGTC ACTGTGCTGT TTCTCCTGCA 
TCAGCTTTTG CTATTGCTAC AGCTGCAGCA GGGCATGGGA GTCCGCCAGG TTTTAACAAT 
TCTGAACGAA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACTAA TCGTGTCCTG 
AATGTCTTAC GTCACTGGGT CTCCAAGCAC TCTCAGGATT TTGAACTAAA CAATGAGCTG 
AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCTGACCT TCTACCACAA 
GAAAGGAAAG CTACTGCAAA TATACTAAGG GCACTTTCTC AGGACGATCA AGATGATACC 
CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGAATGTC CAAAACCGGA GTGCTTTGAG 
ACTTTGTCTG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGACCACAT AGTTTTCAGA 
AGCATACCCT ATGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAGAGA 
ACACCCTATA TTATGAAAAC TAGTCAACAT TTTAATGATA TGAGTAACCT TGTTGCCTCC 
CAAATAATGA ATTATGCTGA TGTCAGCTCC CGGGCTAACT CAATTGAAAA GTGGGTAGCA 
GTAGCTGATA TTTGTCGATG TATGCACAAT TACAATGGTG TGTTAGAAAT CACGTCAGCC 
TTGAACAGAA GTGCAATCTA CAGACTGAAG AAAACTTGGG CTAAGGTATC CAAACAGGCA 
AAAGCACTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAATCTT 
AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCAT ACCTTGGAAT GTATTTGACA 
GATCTAGCAT TTATTGAAGA AGGAACACCA AACTTCACTG AGGAAGGCCT TGTTAATTTT 
TCCAAGATGA GAATGATCTC ACACATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC 
TACCGCATAG AACATCAGCA GAAGGTCACT CACTACTTAC TCGACAAGGC ACTCATCATA 
GATGAAGATA CTTTGTATGA GCTTTCCCTG AAGCTTGAAC CCAGACTCCC CGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa48314
Clone ID Related Accession (Same CDS sequence) XM_015280709.1 , XM_015280709.2
Accession Version XM_015280709.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA 
GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC 
ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA 
GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT 
GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT 
GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC 
TGGGTCTCCA AGCACTCTCA GGATTTTGAA CTAAACAATG AGCTGAAAAT GAATGTGTTA 
AATTTGCTGG AAGAAGTTTT GAGAGACCCT GACCTTCTAC CACAAGAAAG GAAAGCTACT 
GCAAATATAC TAAGGGCACT TTCTCAGGAC GATCAAGATG ATACCCATTT AAAAATAGAG 
GATATCATTC AGATGTCAGA ATGTCCAAAA CCGGAGTGCT TTGAGACTTT GTCTGCCATG 
GAGCTTGCAG AGCAAATAAC TCTTTTAGAC CACATAGTTT TCAGAAGCAT ACCCTATGAA 
GAGTTTCTTG GGCAGGGCTG GATGAAACTG GATAAAAATG AGAGAACACC CTATATTATG 
AAAACTAGTC AACATTTTAA TGATATGAGT AACCTTGTTG CCTCCCAAAT AATGAATTAT 
GCTGATGTCA GCTCCCGGGC TAACTCAATT GAAAAGTGGG TAGCAGTAGC TGATATTTGT 
CGATGTATGC ACAATTACAA TGGTGTGTTA GAAATCACGT CAGCCTTGAA CAGAAGTGCA 
ATCTACAGAC TGAAGAAAAC TTGGGCTAAG GTATCCAAAC AGGCAAAAGC ACTCATGGAC 
AAGCTTCAGA AGACTGTGTC ATCTGAAGGA AGATTTAAAA ATCTTAGAGA AACACTCAAA 
AATTGTAACC CACCTGCTGT TCCATACCTT GGAATGTATT TGACAGATCT AGCATTTATT 
GAAGAAGGAA CACCAAACTT CACTGAGGAA GGCCTTGTTA ATTTTTCCAA GATGAGAATG 
ATCTCACACA TTATCAGGGA AATACGCCAG TTCCAGCAGA CCTCCTACCG CATAGAACAT 
CAGCAGAAGG TCACTCACTA CTTACTCGAC AAGGCACTCA TCATAGATGA AGATACTTTG 
TATGAGCTTT CCCTGAAGCT TGAACCCAGA CTCCCCGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136195.1
CDS303..4124
Translation

Target ORF information:

RefSeq Version XM_015280709.1
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015280709.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA 
GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC 
ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA 
GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT 
GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT 
GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC 
TGGGTCTCCA AGCACTCTCA GGATTTTGAA CTAAACAATG AGCTGAAAAT GAATGTGTTA 
AATTTGCTGG AAGAAGTTTT GAGAGACCCT GACCTTCTAC CACAAGAAAG GAAAGCTACT 
GCAAATATAC TAAGGGCACT TTCTCAGGAC GATCAAGATG ATACCCATTT AAAAATAGAG 
GATATCATTC AGATGTCAGA ATGTCCAAAA CCGGAGTGCT TTGAGACTTT GTCTGCCATG 
GAGCTTGCAG AGCAAATAAC TCTTTTAGAC CACATAGTTT TCAGAAGCAT ACCCTATGAA 
GAGTTTCTTG GGCAGGGCTG GATGAAACTG GATAAAAATG AGAGAACACC CTATATTATG 
AAAACTAGTC AACATTTTAA TGATATGAGT AACCTTGTTG CCTCCCAAAT AATGAATTAT 
GCTGATGTCA GCTCCCGGGC TAACTCAATT GAAAAGTGGG TAGCAGTAGC TGATATTTGT 
CGATGTATGC ACAATTACAA TGGTGTGTTA GAAATCACGT CAGCCTTGAA CAGAAGTGCA 
ATCTACAGAC TGAAGAAAAC TTGGGCTAAG GTATCCAAAC AGGCAAAAGC ACTCATGGAC 
AAGCTTCAGA AGACTGTGTC ATCTGAAGGA AGATTTAAAA ATCTTAGAGA AACACTCAAA 
AATTGTAACC CACCTGCTGT TCCATACCTT GGAATGTATT TGACAGATCT AGCATTTATT 
GAAGAAGGAA CACCAAACTT CACTGAGGAA GGCCTTGTTA ATTTTTCCAA GATGAGAATG 
ATCTCACACA TTATCAGGGA AATACGCCAG TTCCAGCAGA CCTCCTACCG CATAGAACAT 
CAGCAGAAGG TCACTCACTA CTTACTCGAC AAGGCACTCA TCATAGATGA AGATACTTTG 
TATGAGCTTT CCCTGAAGCT TGAACCCAGA CTCCCCGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa48314
Clone ID Related Accession (Same CDS sequence) XM_015280709.1 , XM_015280709.2
Accession Version XM_015280709.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280709.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA 
GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC 
ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA 
GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT 
GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT 
GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC 
TGGGTCTCCA AGCACTCTCA GGATTTTGAA CTAAACAATG AGCTGAAAAT GAATGTGTTA 
AATTTGCTGG AAGAAGTTTT GAGAGACCCT GACCTTCTAC CACAAGAAAG GAAAGCTACT 
GCAAATATAC TAAGGGCACT TTCTCAGGAC GATCAAGATG ATACCCATTT AAAAATAGAG 
GATATCATTC AGATGTCAGA ATGTCCAAAA CCGGAGTGCT TTGAGACTTT GTCTGCCATG 
GAGCTTGCAG AGCAAATAAC TCTTTTAGAC CACATAGTTT TCAGAAGCAT ACCCTATGAA 
GAGTTTCTTG GGCAGGGCTG GATGAAACTG GATAAAAATG AGAGAACACC CTATATTATG 
AAAACTAGTC AACATTTTAA TGATATGAGT AACCTTGTTG CCTCCCAAAT AATGAATTAT 
GCTGATGTCA GCTCCCGGGC TAACTCAATT GAAAAGTGGG TAGCAGTAGC TGATATTTGT 
CGATGTATGC ACAATTACAA TGGTGTGTTA GAAATCACGT CAGCCTTGAA CAGAAGTGCA 
ATCTACAGAC TGAAGAAAAC TTGGGCTAAG GTATCCAAAC AGGCAAAAGC ACTCATGGAC 
AAGCTTCAGA AGACTGTGTC ATCTGAAGGA AGATTTAAAA ATCTTAGAGA AACACTCAAA 
AATTGTAACC CACCTGCTGT TCCATACCTT GGAATGTATT TGACAGATCT AGCATTTATT 
GAAGAAGGAA CACCAAACTT CACTGAGGAA GGCCTTGTTA ATTTTTCCAA GATGAGAATG 
ATCTCACACA TTATCAGGGA AATACGCCAG TTCCAGCAGA CCTCCTACCG CATAGAACAT 
CAGCAGAAGG TCACTCACTA CTTACTCGAC AAGGCACTCA TCATAGATGA AGATACTTTG 
TATGAGCTTT CCCTGAAGCT TGAACCCAGA CTCCCCGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136195.1
CDS295..4116
Translation

Target ORF information:

RefSeq Version XM_015280709.2
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015280709.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA 
GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC 
ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA 
GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT 
GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT 
GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC 
TGGGTCTCCA AGCACTCTCA GGATTTTGAA CTAAACAATG AGCTGAAAAT GAATGTGTTA 
AATTTGCTGG AAGAAGTTTT GAGAGACCCT GACCTTCTAC CACAAGAAAG GAAAGCTACT 
GCAAATATAC TAAGGGCACT TTCTCAGGAC GATCAAGATG ATACCCATTT AAAAATAGAG 
GATATCATTC AGATGTCAGA ATGTCCAAAA CCGGAGTGCT TTGAGACTTT GTCTGCCATG 
GAGCTTGCAG AGCAAATAAC TCTTTTAGAC CACATAGTTT TCAGAAGCAT ACCCTATGAA 
GAGTTTCTTG GGCAGGGCTG GATGAAACTG GATAAAAATG AGAGAACACC CTATATTATG 
AAAACTAGTC AACATTTTAA TGATATGAGT AACCTTGTTG CCTCCCAAAT AATGAATTAT 
GCTGATGTCA GCTCCCGGGC TAACTCAATT GAAAAGTGGG TAGCAGTAGC TGATATTTGT 
CGATGTATGC ACAATTACAA TGGTGTGTTA GAAATCACGT CAGCCTTGAA CAGAAGTGCA 
ATCTACAGAC TGAAGAAAAC TTGGGCTAAG GTATCCAAAC AGGCAAAAGC ACTCATGGAC 
AAGCTTCAGA AGACTGTGTC ATCTGAAGGA AGATTTAAAA ATCTTAGAGA AACACTCAAA 
AATTGTAACC CACCTGCTGT TCCATACCTT GGAATGTATT TGACAGATCT AGCATTTATT 
GAAGAAGGAA CACCAAACTT CACTGAGGAA GGCCTTGTTA ATTTTTCCAA GATGAGAATG 
ATCTCACACA TTATCAGGGA AATACGCCAG TTCCAGCAGA CCTCCTACCG CATAGAACAT 
CAGCAGAAGG TCACTCACTA CTTACTCGAC AAGGCACTCA TCATAGATGA AGATACTTTG 
TATGAGCTTT CCCTGAAGCT TGAACCCAGA CTCCCCGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa48315
Clone ID Related Accession (Same CDS sequence) XM_015280710.2 , XM_015280710.1
Accession Version XM_015280710.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGTT 
CTGGAATCAG TGTCAGTGGA CAGAACTATT CCTGAAAGCA CCTCGGCAGT GGATACCACA 
GAACTTTCCC CGTGTAGATC CCCTTCAACA CCACGTCATC TCCGCTATCG TCAATCAGGA 
GTGCAAACTG CTGACAACAG TCACTGTGCT GTTTCTCCTG CATCAGCTTT TGCTATTGCT 
ACAGCTGCAG CAGGGCATGG GAGTCCGCCA GGTTTTAACA ATTCTGAACG AATGTGTGAC 
AAAGAGTTCA TAATACGCAG AGCAGCCACT AATCGTGTCC TGAATGTCTT ACGTCACTGG 
GTCTCCAAGC ACTCTCAGGA TTTTGAACTA AACAATGAGC TGAAAATGAA TGTGTTAAAT 
TTGCTGGAAG AAGTTTTGAG AGACCCTGAC CTTCTACCAC AAGAAAGGAA AGCTACTGCA 
AATATACTAA GGGCACTTTC TCAGGACGAT CAAGATGATA CCCATTTAAA AATAGAGGAT 
ATCATTCAGA TGTCAGAATG TCCAAAACCG GAGTGCTTTG AGACTTTGTC TGCCATGGAG 
CTTGCAGAGC AAATAACTCT TTTAGACCAC ATAGTTTTCA GAAGCATACC CTATGAAGAG 
TTTCTTGGGC AGGGCTGGAT GAAACTGGAT AAAAATGAGA GAACACCCTA TATTATGAAA 
ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CCCAAATAAT GAATTATGCT 
GATGTCAGCT CCCGGGCTAA CTCAATTGAA AAGTGGGTAG CAGTAGCTGA TATTTGTCGA 
TGTATGCACA ATTACAATGG TGTGTTAGAA ATCACGTCAG CCTTGAACAG AAGTGCAATC 
TACAGACTGA AGAAAACTTG GGCTAAGGTA TCCAAACAGG CAAAAGCACT CATGGACAAG 
CTTCAGAAGA CTGTGTCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC ACTCAAAAAT 
TGTAACCCAC CTGCTGTTCC ATACCTTGGA ATGTATTTGA CAGATCTAGC ATTTATTGAA 
GAAGGAACAC CAAACTTCAC TGAGGAAGGC CTTGTTAATT TTTCCAAGAT GAGAATGATC 
TCACACATTA TCAGGGAAAT ACGCCAGTTC CAGCAGACCT CCTACCGCAT AGAACATCAG 
CAGAAGGTCA CTCACTACTT ACTCGACAAG GCACTCATCA TAGATGAAGA TACTTTGTAT 
GAGCTTTCCC TGAAGCTTGA ACCCAGACTC CCCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136196.1
CDS295..4113
Translation

Target ORF information:

RefSeq Version XM_015280710.2
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015280710.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGTT 
CTGGAATCAG TGTCAGTGGA CAGAACTATT CCTGAAAGCA CCTCGGCAGT GGATACCACA 
GAACTTTCCC CGTGTAGATC CCCTTCAACA CCACGTCATC TCCGCTATCG TCAATCAGGA 
GTGCAAACTG CTGACAACAG TCACTGTGCT GTTTCTCCTG CATCAGCTTT TGCTATTGCT 
ACAGCTGCAG CAGGGCATGG GAGTCCGCCA GGTTTTAACA ATTCTGAACG AATGTGTGAC 
AAAGAGTTCA TAATACGCAG AGCAGCCACT AATCGTGTCC TGAATGTCTT ACGTCACTGG 
GTCTCCAAGC ACTCTCAGGA TTTTGAACTA AACAATGAGC TGAAAATGAA TGTGTTAAAT 
TTGCTGGAAG AAGTTTTGAG AGACCCTGAC CTTCTACCAC AAGAAAGGAA AGCTACTGCA 
AATATACTAA GGGCACTTTC TCAGGACGAT CAAGATGATA CCCATTTAAA AATAGAGGAT 
ATCATTCAGA TGTCAGAATG TCCAAAACCG GAGTGCTTTG AGACTTTGTC TGCCATGGAG 
CTTGCAGAGC AAATAACTCT TTTAGACCAC ATAGTTTTCA GAAGCATACC CTATGAAGAG 
TTTCTTGGGC AGGGCTGGAT GAAACTGGAT AAAAATGAGA GAACACCCTA TATTATGAAA 
ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CCCAAATAAT GAATTATGCT 
GATGTCAGCT CCCGGGCTAA CTCAATTGAA AAGTGGGTAG CAGTAGCTGA TATTTGTCGA 
TGTATGCACA ATTACAATGG TGTGTTAGAA ATCACGTCAG CCTTGAACAG AAGTGCAATC 
TACAGACTGA AGAAAACTTG GGCTAAGGTA TCCAAACAGG CAAAAGCACT CATGGACAAG 
CTTCAGAAGA CTGTGTCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC ACTCAAAAAT 
TGTAACCCAC CTGCTGTTCC ATACCTTGGA ATGTATTTGA CAGATCTAGC ATTTATTGAA 
GAAGGAACAC CAAACTTCAC TGAGGAAGGC CTTGTTAATT TTTCCAAGAT GAGAATGATC 
TCACACATTA TCAGGGAAAT ACGCCAGTTC CAGCAGACCT CCTACCGCAT AGAACATCAG 
CAGAAGGTCA CTCACTACTT ACTCGACAAG GCACTCATCA TAGATGAAGA TACTTTGTAT 
GAGCTTTCCC TGAAGCTTGA ACCCAGACTC CCCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa48315
Clone ID Related Accession (Same CDS sequence) XM_015280710.2 , XM_015280710.1
Accession Version XM_015280710.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGTT 
CTGGAATCAG TGTCAGTGGA CAGAACTATT CCTGAAAGCA CCTCGGCAGT GGATACCACA 
GAACTTTCCC CGTGTAGATC CCCTTCAACA CCACGTCATC TCCGCTATCG TCAATCAGGA 
GTGCAAACTG CTGACAACAG TCACTGTGCT GTTTCTCCTG CATCAGCTTT TGCTATTGCT 
ACAGCTGCAG CAGGGCATGG GAGTCCGCCA GGTTTTAACA ATTCTGAACG AATGTGTGAC 
AAAGAGTTCA TAATACGCAG AGCAGCCACT AATCGTGTCC TGAATGTCTT ACGTCACTGG 
GTCTCCAAGC ACTCTCAGGA TTTTGAACTA AACAATGAGC TGAAAATGAA TGTGTTAAAT 
TTGCTGGAAG AAGTTTTGAG AGACCCTGAC CTTCTACCAC AAGAAAGGAA AGCTACTGCA 
AATATACTAA GGGCACTTTC TCAGGACGAT CAAGATGATA CCCATTTAAA AATAGAGGAT 
ATCATTCAGA TGTCAGAATG TCCAAAACCG GAGTGCTTTG AGACTTTGTC TGCCATGGAG 
CTTGCAGAGC AAATAACTCT TTTAGACCAC ATAGTTTTCA GAAGCATACC CTATGAAGAG 
TTTCTTGGGC AGGGCTGGAT GAAACTGGAT AAAAATGAGA GAACACCCTA TATTATGAAA 
ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CCCAAATAAT GAATTATGCT 
GATGTCAGCT CCCGGGCTAA CTCAATTGAA AAGTGGGTAG CAGTAGCTGA TATTTGTCGA 
TGTATGCACA ATTACAATGG TGTGTTAGAA ATCACGTCAG CCTTGAACAG AAGTGCAATC 
TACAGACTGA AGAAAACTTG GGCTAAGGTA TCCAAACAGG CAAAAGCACT CATGGACAAG 
CTTCAGAAGA CTGTGTCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC ACTCAAAAAT 
TGTAACCCAC CTGCTGTTCC ATACCTTGGA ATGTATTTGA CAGATCTAGC ATTTATTGAA 
GAAGGAACAC CAAACTTCAC TGAGGAAGGC CTTGTTAATT TTTCCAAGAT GAGAATGATC 
TCACACATTA TCAGGGAAAT ACGCCAGTTC CAGCAGACCT CCTACCGCAT AGAACATCAG 
CAGAAGGTCA CTCACTACTT ACTCGACAAG GCACTCATCA TAGATGAAGA TACTTTGTAT 
GAGCTTTCCC TGAAGCTTGA ACCCAGACTC CCCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136196.1
CDS303..4121
Translation

Target ORF information:

RefSeq Version XM_015280710.1
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015280710.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGTT 
CTGGAATCAG TGTCAGTGGA CAGAACTATT CCTGAAAGCA CCTCGGCAGT GGATACCACA 
GAACTTTCCC CGTGTAGATC CCCTTCAACA CCACGTCATC TCCGCTATCG TCAATCAGGA 
GTGCAAACTG CTGACAACAG TCACTGTGCT GTTTCTCCTG CATCAGCTTT TGCTATTGCT 
ACAGCTGCAG CAGGGCATGG GAGTCCGCCA GGTTTTAACA ATTCTGAACG AATGTGTGAC 
AAAGAGTTCA TAATACGCAG AGCAGCCACT AATCGTGTCC TGAATGTCTT ACGTCACTGG 
GTCTCCAAGC ACTCTCAGGA TTTTGAACTA AACAATGAGC TGAAAATGAA TGTGTTAAAT 
TTGCTGGAAG AAGTTTTGAG AGACCCTGAC CTTCTACCAC AAGAAAGGAA AGCTACTGCA 
AATATACTAA GGGCACTTTC TCAGGACGAT CAAGATGATA CCCATTTAAA AATAGAGGAT 
ATCATTCAGA TGTCAGAATG TCCAAAACCG GAGTGCTTTG AGACTTTGTC TGCCATGGAG 
CTTGCAGAGC AAATAACTCT TTTAGACCAC ATAGTTTTCA GAAGCATACC CTATGAAGAG 
TTTCTTGGGC AGGGCTGGAT GAAACTGGAT AAAAATGAGA GAACACCCTA TATTATGAAA 
ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CCCAAATAAT GAATTATGCT 
GATGTCAGCT CCCGGGCTAA CTCAATTGAA AAGTGGGTAG CAGTAGCTGA TATTTGTCGA 
TGTATGCACA ATTACAATGG TGTGTTAGAA ATCACGTCAG CCTTGAACAG AAGTGCAATC 
TACAGACTGA AGAAAACTTG GGCTAAGGTA TCCAAACAGG CAAAAGCACT CATGGACAAG 
CTTCAGAAGA CTGTGTCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC ACTCAAAAAT 
TGTAACCCAC CTGCTGTTCC ATACCTTGGA ATGTATTTGA CAGATCTAGC ATTTATTGAA 
GAAGGAACAC CAAACTTCAC TGAGGAAGGC CTTGTTAATT TTTCCAAGAT GAGAATGATC 
TCACACATTA TCAGGGAAAT ACGCCAGTTC CAGCAGACCT CCTACCGCAT AGAACATCAG 
CAGAAGGTCA CTCACTACTT ACTCGACAAG GCACTCATCA TAGATGAAGA TACTTTGTAT 
GAGCTTTCCC TGAAGCTTGA ACCCAGACTC CCCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa48316
Clone ID Related Accession (Same CDS sequence) XM_015280711.2 , XM_015280711.1
Accession Version XM_015280711.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA 
GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC 
ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA 
GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT 
GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT 
GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC 
TGGGTCTCCA AGCACTCTCA GGTAGCATGT ATAGGTTTGT GGAAGTTGGA GATCTTCGAA 
GATCTCAGCA GGTTCTTCAT GATTACTCCT TGTACAATTC CATTGCCTAA CCAATTGAGT 
GGCATTCTGC TGTGGAGTGA TGGCATCAGT GGACAAAGAA GGGGTAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136197.1
CDS302..3352
Translation

Target ORF information:

RefSeq Version XM_015280711.2
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015280711.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA 
GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC 
ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA 
GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT 
GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT 
GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC 
TGGGTCTCCA AGCACTCTCA GGTAGCATGT ATAGGTTTGT GGAAGTTGGA GATCTTCGAA 
GATCTCAGCA GGTTCTTCAT GATTACTCCT TGTACAATTC CATTGCCTAA CCAATTGAGT 
GGCATTCTGC TGTGGAGTGA TGGCATCAGT GGACAAAGAA GGGGTAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa48316
Clone ID Related Accession (Same CDS sequence) XM_015280711.2 , XM_015280711.1
Accession Version XM_015280711.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product ras-specific guanine nucleotide-releasing factor 2 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA 
GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC 
ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA 
GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT 
GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT 
GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC 
TGGGTCTCCA AGCACTCTCA GGTAGCATGT ATAGGTTTGT GGAAGTTGGA GATCTTCGAA 
GATCTCAGCA GGTTCTTCAT GATTACTCCT TGTACAATTC CATTGCCTAA CCAATTGAGT 
GGCATTCTGC TGTGGAGTGA TGGCATCAGT GGACAAAGAA GGGGTAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136197.1
CDS310..3360
Translation

Target ORF information:

RefSeq Version XM_015280711.1
Organism Gallus gallus(chicken)
Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015280711.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC 
GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC 
CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG 
GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT 
TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA 
GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA 
GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC 
AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA 
ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT 
GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT 
AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG 
AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA 
CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG 
CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT 
CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT 
CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG 
AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT 
ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC 
ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT 
ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT 
GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT 
GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA 
GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT 
CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG 
AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA 
AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC 
ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT 
GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC 
CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT 
GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT 
GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC 
AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT 
TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT 
ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA 
CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC 
AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT 
CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG 
ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC 
AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT 
CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC 
TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA 
GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC 
ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA 
GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT 
GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT 
GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC 
TGGGTCTCCA AGCACTCTCA GGTAGCATGT ATAGGTTTGT GGAAGTTGGA GATCTTCGAA 
GATCTCAGCA GGTTCTTCAT GATTACTCCT TGTACAATTC CATTGCCTAA CCAATTGAGT 
GGCATTCTGC TGTGGAGTGA TGGCATCAGT GGACAAAGAA GGGGTAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed