cacna1h cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1h
Entrez Gene ID	100494894
Full Name	calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms	ca(v)3.2,cav3.2
General protein information	Preferred Names calcium channel, voltage-dependent, T type, alpha 1H subunit Names voltage-dependent T-type calcium channel subunit alpha-1H
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031892924.1	XP_031748784.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
XM_031892925.1	XP_031748785.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
XM_018097254.1	XP_017952743.1	voltage-dependent T-type calcium channel subunit alpha-1H
XM_031892923.1	XP_031748783.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC560875	XP_003198197.2
Homo sapiens (human)	CACNA1H	NP_066921.2
Bos taurus (cattle)	CACNA1H	XP_002698020.3
Mus musculus (house mouse)	Cacna1h	NP_067390.4
Rattus norvegicus (Norway rat)	Cacna1h	NP_722521.1
Gallus gallus (chicken)	CACNA1H	XP_414830.4
Xenopus tropicalis (tropical clawed frog)	LOC100494894	XP_002932520.2
Pan troglodytes (chimpanzee)	CACNA1H	XP_001172468.2
Canis lupus familiaris (dog)	CACNA1H	XP_537016.2

Gene Ontology

The following cacna1h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa66326	XM_031892923.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa66327	XM_031892924.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa66328	XM_031892925.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa46699	XM_018097254.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1h ( XM_031892923.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748783.1 Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa66326
Clone ID Related Accession (Same CDS sequence)	XM_031892923.1
Accession Version	XM_031892923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGATG GCGAGAGGAA AGAGCCCTTC CAGCCACTGG CCGGAGACGA TATCCGGGTG 
CCCATACCCC CGACACCCCA AGCAGGGGAC CCCAGTGAAG AAGAGGATGC GGGAAATGCA 
GAGAAGTCAG CTCTGGGCAG CTTGGGCTCT GGAGGTTCTT CTGGAAGAGG TCGTGAACAA 
GGGATGGGCT CGGAGGAAGG AGAACAGGTT CCATACCCAG CCCTGGCACC CACCGTCTTC 
TTCTGTCTCA GCCAAACCAC GCGTCCACGG AACTGGTGCC TCAGACTGGT CTGTAATCCA 
TGGTTCGAGC ATGTCAGCAT GCTGGTCATT TTGCTGAACT GCGTCACCCT GGGGATGTTC 
CAGCCCTGCG AGGATACAAA CTGTATGTCA GAGAGATGCA ACATTCTAGA GGCATTCGAC 
GGCTTCATCT TTGCATTCTT TGCTGTGGAG ATGGTTATTA AGATGGTAGC GCTTGGCATC 
TTTGGGCAGA AGTGCTACCT GGGAGACACA TGGAACTGGC TGGATTTCTT CATTGTTATG 
GCAGGGATGT TGGAATATTC CCTCGATGGA CACAATGTCA GCCTATCCGC TATCCGCACT 
GTACGTGTTT TAAGGCCGCT AAGAGCCATT AACAGGGTTC CCAGTATGAG GATACTGGTG 
ACCCTGCTGC TAGATACTCT GCCTATGTTA GGCAACGTCC TTCTCTTGTG TTTCTTTGTG 
TTTTTCATCT TTGGAATCGT CGGCGTTCAG CTTTGGGCTG GGCTACTGCG CAACAGGTGT 
TTTATGGATA CAAATTTTGT AATGAGTTAC AATTTCACCC CTCTGAGGCC GTATTACCAG 
GTGGATGATG CAGAGGACAA CCCTTATATC TGCTCCTCTA ACCGAGACAA TGGGATGTTA 
AAGTGCCAGG ACATCCCTCC CAAAATGGAC TGCTCCCATG TATACTACTC AGATTTCCCC 
AGTAGGAATA GCTGCGTGAA TATTAATCAA TACTACAACG TATGCAAGCC GGGAGAGTTC 
AATCCACATA AAGGGGCCAT TAATTTTGAT AACATTGGCT ACGCCTGGAT AGCCATTTTT 
CAGGTTATAA CCCTAGAAGG ATGGGTTGAT ATCATGTACT ATGTTATGGA CGCACATTCT 
TTTTACAATT TTATATATTT TATACTACTT ATAATAGTGG GTTCTTTCTT CATGATAAAC 
TTGTGCCTGG TTGTGATAGC AACACAGTTT TCTGAAACAA AGCAGCGAGA GAACCAGCTA 
ATGCAGGAGC AACGGGCTCG TTACCTCTCC AACGACAGCA CAATCGCCAG CTTCTCGGAG 
CCAGGGAGCT GCTATGAGGA GCTGCTCAAA TACATCTGCC ACATCTTCAG GAAGGTTAAG 
CGAAGGACGT TAAGACTTTA TAATAACTGG CAGAATAAGA GGAGGAAAAA AGTTCAGCCC 
AACAGCAACA CGAACAGTCA GGGGCGTCAC CGCAAGTGCA AGCGGCGTAT CACCTCCATT 
CATCACTTAA TACACCACCA CCATCATCAC CACCACCATC ATTACCACAT CAGCAATGGG 
AGTCCCCGGG GTCCCAGGTC TAGTCCGGAG ATCTGTGATG TGGAGATGAA AATCATGAAG 
CCAAAGAATC AACTAAGGCT TCCTCCAGCA TCCCCCAGCC TCCAAAGCAT GTCCCCCAAC 
AGTGAGGCCG TCCACAGTAT CTACCACGCG GACTGCCACT TAGAGGCGCC TCAGCGGAAA 
TCGAGCAAGT CTTCCAATGC CTCGTCAAAC ATAAAACTGA ATGCTGGGTT GAATCACAGT 
AACATGAACT ATCCCACCAT CATGCCCTCC CAAGCCAACA AAACCAGCCC CGCAACACTA 
CCCAAAGGAA AAAGGAATGG CAGCAATGCA GTCAGCTTGC TAAGCAGCCC AGTCAGTATG 
AAGGCAGATT CGTTTGGGAA GACCAACCAC CTAGTCGGAG AGCATGGCCT GTGCAGGAGT 
TCCAGTAAGC TCTCCGGTTT GAGCGTGCCA TGCTCCTTGC CAAGCCCTCA GCCAAGCCCT 
CTACTGTGCG AAATCCAGAA CTGCCCGTAC TGTGCCAACC TTTTGGAGAA CCCTGACTTG 
GAGCTCAGCG ACTCTGAGAG TTGCGAATCG GATTCTAACG GGGTCTATGA ATTCACCCAG 
GACTTGAGGC GTGGAGACCA AAGAGACCAG GTGCAGAAAG GCAGAAAGCT CAAGACTAAG 
CCAAAGGAGG CTAACAAGGT GTATCTGCTC TGGGAAGAGT TTACCGTCCG ACTGAAAAGG 
ATTGTGGACA GCAAATATTT TAACCGCGGG ATTATGATTG CCATCCTGAT CAACACGCTG 
AGCATGGGGA TTGAGTATCA TGAGCAGCCA GAAGAACTGA CTAACGCCTT GGAAATCAGC 
AATATTGTGT TTACAAGCAT GTTTGCGCTG GAGATGCTGC TGAAACTCTT GGCCTTTGGG 
ATCTTTCAAT ACATCAAGAA TCCATACAAC ATCTTTGATG GGATCATTGT CGTCATCAGT 
GTGTGGGAAA TCATCGGTCA GTCGGACGGA GGTCTGTCTG TGCTTAGAAC ATTTCGCCTC 
CTTCGTGTGC TGAAGCTGGT ACGATTTATG CCAGCACTGC GGCGTCAGCT GGTGGTCCTG 
GTGAAGACGA TGGACAACGT TGCCACCTTT TGCATGCTTC TTATGCTCTT CATCTTTATA 
TTCAGCATCC TTGGCATGCA TCTGTTTGGG TGTAAGTTTA CTTGGACTGC AGACACCGGA 
GAGACAGTCA AAGACAGAAA GAACTTTGAT TCTTTGCTTT GGGCGATTGT CACCGTCTTC 
CAGATTCTCA CCCAAGAAGA CTGGAACATG GTCCTATACA ATGGCATGGC TTCCACCTCC 
TCCTGGGCAG CTCTCTACTT TGTGGCCCTG ATGACATTTG GAAATTATGT GCTTTTCAAC 
TTGTTAGTAG CCATCTTAGT CGAAGGCTTC CAAGCAGAGG GAGATGCAAC AAGGTCAGAT 
ACAGAGGAGG ACAAGGCTTC AGGCAACTTT GATGAAGATC TGGAGAAACT GAAGGAGCTT 
CGTGCTGCAG AACTGAAGAT GTACTCACTC GCAGTGACAC CCAACGGACA CCTGGACCCT 
CGGAGTTCCA TGCCCCCACC TATCATCATG CGCACCGCCG CCACCCCAAT GCCTACACCA 
AAGAGCTCCC CTTGCATGGA CTCATCACAT CCGTTCGGCG ATTCAAGAAG GGAAAGTAGC 
GTCTCGATAG ATCCATCAAT CGTTGATCAG AAGTCTTTGG CAAGTCTACG CAGCACCCCT 
TGTACCCCCT GGGGCACTTA CAGCAACTGG GGAAGCCGTC GCTCTAGCTG GAATAGTCTG 
GGTAGAGCCC CAAGCATCAA AAAGAAGAGC CAATCAGGGG AGAGGGAGTC CTTACTCTCT 
GGTGAAGGAA AGGGGAGCAC AGATGATGAC TCAGATGACT GCAAGTCAAG CCCAGCGGCG 
AGGGTATCTC TCCATCGCAG GGCAGGTTCC CTGGACACGC GGGGCTCTTT GGATATCCCA 
GATTTCCTGC AGGTGCCCAC GTTACGCCAT TCCCTAAGTG TCAGTCCTTT GGCCATGGTT 
CCTTCAGATT ATCAAGATTG CAATGGGAAA ATGATGCACG TGCCCAGTGA TCTGTTCGTT 
CGGGTGGAGA AACACAGGGA GGATCCGTTG GAGTATGAAG ACGACATAGA AGATAGTTAC 
TGTTTCCGAA TCCGAAAGAT GTTGGAGCCA TACAAACCTA CATGGTGTAA GACGCATGAA 
GACTGGTCCC TCTACATGTT CTCACCTCAA AACCAGTTCC GGATGATGTG CCAAAAAGTC 
ATTGCCCACA AGATGTTTGA CCACGTGGTT CTTGTCTTCA TCTTCTTAAA CTGCATCACC 
ATTGCACTTG AACGGCCTGA TATTGACCCC AATAGCCTGG AACGGATATT CTTGAGTGTC 
TCCAATTACA TTTTTACTGC AATCTTTGTT TTGGAAATGG CAATTAAGGT GGTGGCCCTG 
GGCTTTGTCT CCGGGGAGAA CACTTACCTG CAGAGCAGCT GGAACGTGCT TGATGGGATT 
CTGGTCTTCG TTTCCATCAT CGATATCGTC GTGTCCCTTG CCTCTGCCGG GGGGGCAAAG 
ATCCTGGGAG TCTTGCGGGT ACTGAGGTTG CTGCGGACCC TGCGCCCACT TAGGGTTATC 
AGCCGAGCCC CGGGCCTGAA GTTGGTGGTA GAAACTCTCA TTTCCTCCCT GAGGCCTATC 
GGGAACATTG TCCTTATCTG CTGCGCTTTC TTCATCATCT TTGGCATTCT GGGAGTACAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCGAA GGGTTAGAGA CGAGGAACAT CACCAACAGG 
TCAGAGTGCA CCCGGGCACG GTACAAATGG GTGCGGCGCA AATACAACTT TGACAACCTT 
GGCCAGGCGC TGATGTCGCT CTTTGTCCTC TCCTCCAAAG ATGGCTGGGT GAATATCATG 
TACGATGGCC TGGATGCCGT TGCCACAGAT GTTCAGCCAG TCAAAAACCA CAACCCATGG 
ATGCTCCTGT ACTTCATCTC CTTCCTCCTC ATCGTCAGCT TCTTCGTGCT CAACATGTTC 
GTAGGGGTGG TGGTAGAGAA CTTCCACAAG TGCCGGCAAC ACCAGGAGGC GGAAGAGGCC 
CGGCGCCGGG AGGAGAAACG CCTCCGCCGC CTGGAGAAAA AGCGCAGGAG TAAGGCGTTG 
TATATGTCTG AGGCCCAGCG GAGACCGTAT TACGCAGAGT ACTCGCCGGC GCGCCTCTAC 
ATCCACACCA TGTGTACCAG CCACTACCTG GATCTCTTTA TCACCTGCAT TATTTGTATC 
AACGTCATCA CCATGTCCAT GGAACACTAC AACCAACCCA AGTCCCTGGA TGAAGCTTTA 
AAATACTGCA ATTACGTGTT CACCATCGTC TTCGTGTTCG AGGCCCTGTT CAAGCTTGTG 
GCATTTGGGT TCCGGAGGTT TTTTAAAGAC AGGTGGAACC AACTGGACTT GGCCATTGTT 
TTGCTCTCCA TCATGGGAAT CACGTTAGAG GAGATTGAGA TTAACGCCTC CCTTCCCATC 
AATCCAACTA TTATACGCAT CATGAGGGTG CTGAGGATTG CTCGAGTGTT AAAGCTCCTG 
AAAATGGCCA CCGGGATGCG AGCTTTGCTG GATACTGTTG TGCAAGCGTT ACCTCAGGTA 
GGCAACCTTG GCCTGCTCTT CATGCTCCTG TTCTTCATCT ACGCAGCCCT GGGAGTGGAG 
CTGTTCGGAA AGCTCGTGTG CAATGAGGAG AATCCCTGTG AGGGACTCAG CCGACATGCA 
ACATTTGAGA ACTTTGGGAT GGCTTTTCTG ACCCTCTTCC GTGTTTCCAC AGGGGATAAC 
TGGAATGGCA TCATGAAGGA CACTCTACGA GAGTGCAACA GCGATGACAA GAACTGTCTC 
ACCTACCTGC CCGTCATCTC TCCCGTCTAT TTCGTAACCT TCGTGCTCAT TGCCCAGTTT 
GTCTTGGTCA ATGTGGTCGT GGCTGTGCTC ATGAAACATC TAGAGGAGAG CAATAAAGAG 
GCCAAAGAAG ATGCCGAGAT GGATGCAGAG ATTGAGATGG AGATGTCTAG GGCACTGACA 
CCTATAAGTG GTGGCTGCAC TCCAGCTTCA GATGGCAAGG GAACTGAGGG AGGGCAAGAT 
CCATTAAAGG GGGAGCTGCT GAAACAAGTG GGGGGGGCAG TGTGCCAAGA GAATGTGACC 
TTAGTGATCC CAGAGTCAAT GGAGGGTGCT GCCCTGCTGA ACGACGCAGT CCCTGCTTGC 
TCCATATACC AACAGCAGGC CCTGCCACTT CCCCTCCAGC CTTGCGTCCT TGGGCACGAG 
GATGGGGATT TCCAAAGGAG GGATGGATCG GGCTCCCGAA ACCTACTGGC TGTCAAAAAG 
ATCTCGGTGT CTAGGATGCT CTCCTTACCT AATGACAGCT ACATGTTTCG CCCAGTGCTA 
CCAGCAAAGT CATCTTATCC TTTGCAAGAA GTGGAGACCA CTGAAGATAA TTCACCACAT 
GGGTCGACTG CTTCACTCCA CTCCCAGCCA GGGGACAATA ACTCATTGCT GAATATACCA 
GTGGAGTGCC AGCCACAGGC CTCTGTTAGC AACCACCAGG ACTCTGCCGA CCTGCCTAAA 
ATCCCACCGC CACATACATC CTCAAGATCT CCAAGTCTAA ACAGATCCCT GAGAAGACAG 
GAGGCTATAC GTATGGACTC AGTGGATCAG GGTGGTGATT CTAAGGACCT TAATGTGGAA 
TCTACAGAGA GTTTGCATCT GGGAGCTCCA TCAGTTACTA CTTTAGTTCC AAATTTGCTT 
TGCACCTTAA ATGTGTCTCT GCAGGGAACC CCCCAGTCCT CACCTAGGCT ATCTCCACAG 
CCTAGACATT CCAGTGTCCT CACACACAAC CATGTATACA GCCAACACAG TATTCCAAGT 
CGGCCTTCGA GTGTCAGTAG TGGCAACAGC AACCCAGAGG AATCTATGTT TGAGTCCTCA 
GACCTGGCTG ATGAAGAAGT CAGCCATATA AACAGTTCAG CAAAAGACTG GAGAGGATCA 
TCTAATAATA GAAGTCACTC TGTTTCTCCA TTCATCTCCC AATCTTCATC TCCTCCTCCT 
CTTTCTCCGT CACTAAAGAA CTGCAATAGC ACAGTCAGCC TGGCAGGGTC CAGCGGCAAA 
GATAAAGACT TGAAGAAGTT CTATAGTGTT GATACTCAAG GATTCTTAGC TAAGCCCTGC 
TGGGGGGATG ACCAAAGGAG GCACTCAATT GAGATCTGCC CTTCCATTGA TGATGGAGAT 
TCCTGCTTTG ACCTGTCCAT GGAGGACAAA CATTGCTCTT CTGTTCTCAT TCGGTCTGAC 
CCCGAATACA TCAATGGAGT CCGACGAAAG AAGAAGATGA GTCCCCCTTG CATTTCTATA 
GACCCTCCTA TGGAGGATGA AGGTGGCACA GCTTCCCGTA CCAAAGCATC AGAAAACAGC 
ATGATGTTAC GAAGAAGGAC CCCATCCTGT GAATCTACTG CATACAGGGA CTCTTTAGAT 
CTTAGTGACA ACCAGCTTGG GGACCAAATA TCTAAAACTG ATAGACGAAC CCAGGTGCCC 
TGTCGCAATG AACACTTGAT CATACCCAGC TACTCGTTTG ACAAGGTGGA CGGAAACGGC 
TTCTCAGACC TAATATCTAA CAGTGGGGGT TCTACTCCGA CGTCCGTTTT CAATGAACCT 
GTGTCTTTAG AAATGAAACA AAAAGATCAC AACGATCTGA GCGGCGTTTC TAAAGTTTCG 
TTGAAAACTG CACTAGATCA CGTATCCAAC CCAACAGAGG ACATAATCGA TAACCCATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748783.1
CDS	449..7831
Translation	MTDGERKEPFQPLAGDDIRVPIPPTPQAGDPSEEEDAGNAEKSALGSLGSGGSSGRGREQGMGSEEGEQVPYPALAPTVFFCLSQTTRPRNWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKCKRRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSSPEICDVEMKIMKPKNQLRLPPASPSLQSMSPNSEAVHSIYHADCHLEAPQRKSSKSSNASSNIKLNAGLNHSNMNYPTIMPSQANKTSPATLPKGKRNGSNAVSLLSSPVSMKADSFGKTNHLVGEHGLCRSSSKLSGLSVPCSLPSPQPSPLLCEIQNCPYCANLLENPDLELSDSESCESDSNGVYEFTQDLRRGDQRDQVQKGRKLKTKPKEANKVYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTEEDKASGNFDEDLEKLKELRAAELKMYSLAVTPNGHLDPRSSMPPPIIMRTAATPMPTPKSSPCMDSSHPFGDSRRESSVSIDPSIVDQKSLASLRSTPCTPWGTYSNWGSRRSSWNSLGRAPSIKKKSQSGERESLLSGEGKGSTDDDSDDCKSSPAARVSLHRRAGSLDTRGSLDIPDFLQVPTLRHSLSVSPLAMVPSDYQDCNGKMMHVPSDLFVRVEKHREDPLEYEDDIEDSYCFRIRKMLEPYKPTWCKTHEDWSLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKALYMSEAQRRPYYAEYSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNSDDKNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEMSRALTPISGGCTPASDGKGTEGGQDPLKGELLKQVGGAVCQENVTLVIPESMEGAALLNDAVPACSIYQQQALPLPLQPCVLGHEDGDFQRRDGSGSRNLLAVKKISVSRMLSLPNDSYMFRPVLPAKSSYPLQEVETTEDNSPHGSTASLHSQPGDNNSLLNIPVECQPQASVSNHQDSADLPKIPPPHTSSRSPSLNRSLRRQEAIRMDSVDQGGDSKDLNVESTESLHLGAPSVTTLVPNLLCTLNVSLQGTPQSSPRLSPQPRHSSVLTHNHVYSQHSIPSRPSSVSSGNSNPEESMFESSDLADEEVSHINSSAKDWRGSSNNRSHSVSPFISQSSSPPPLSPSLKNCNSTVSLAGSSGKDKDLKKFYSVDTQGFLAKPCWGDDQRRHSIEICPSIDDGDSCFDLSMEDKHCSSVLIRSDPEYINGVRRKKKMSPPCISIDPPMEDEGGTASRTKASENSMMLRRRTPSCESTAYRDSLDLSDNQLGDQISKTDRRTQVPCRNEHLIIPSYSFDKVDGNGFSDLISNSGGSTPTSVFNEPVSLEMKQKDHNDLSGVSKVSLKTALDHVSNPTEDIIDNPL

Target ORF information:

RefSeq Version	XM_031892923.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892923.1

ORF Insert Sequence:

ATGACTGATG GCGAGAGGAA AGAGCCCTTC CAGCCACTGG CCGGAGACGA TATCCGGGTG 
CCCATACCCC CGACACCCCA AGCAGGGGAC CCCAGTGAAG AAGAGGATGC GGGAAATGCA 
GAGAAGTCAG CTCTGGGCAG CTTGGGCTCT GGAGGTTCTT CTGGAAGAGG TCGTGAACAA 
GGGATGGGCT CGGAGGAAGG AGAACAGGTT CCATACCCAG CCCTGGCACC CACCGTCTTC 
TTCTGTCTCA GCCAAACCAC GCGTCCACGG AACTGGTGCC TCAGACTGGT CTGTAATCCA 
TGGTTCGAGC ATGTCAGCAT GCTGGTCATT TTGCTGAACT GCGTCACCCT GGGGATGTTC 
CAGCCCTGCG AGGATACAAA CTGTATGTCA GAGAGATGCA ACATTCTAGA GGCATTCGAC 
GGCTTCATCT TTGCATTCTT TGCTGTGGAG ATGGTTATTA AGATGGTAGC GCTTGGCATC 
TTTGGGCAGA AGTGCTACCT GGGAGACACA TGGAACTGGC TGGATTTCTT CATTGTTATG 
GCAGGGATGT TGGAATATTC CCTCGATGGA CACAATGTCA GCCTATCCGC TATCCGCACT 
GTACGTGTTT TAAGGCCGCT AAGAGCCATT AACAGGGTTC CCAGTATGAG GATACTGGTG 
ACCCTGCTGC TAGATACTCT GCCTATGTTA GGCAACGTCC TTCTCTTGTG TTTCTTTGTG 
TTTTTCATCT TTGGAATCGT CGGCGTTCAG CTTTGGGCTG GGCTACTGCG CAACAGGTGT 
TTTATGGATA CAAATTTTGT AATGAGTTAC AATTTCACCC CTCTGAGGCC GTATTACCAG 
GTGGATGATG CAGAGGACAA CCCTTATATC TGCTCCTCTA ACCGAGACAA TGGGATGTTA 
AAGTGCCAGG ACATCCCTCC CAAAATGGAC TGCTCCCATG TATACTACTC AGATTTCCCC 
AGTAGGAATA GCTGCGTGAA TATTAATCAA TACTACAACG TATGCAAGCC GGGAGAGTTC 
AATCCACATA AAGGGGCCAT TAATTTTGAT AACATTGGCT ACGCCTGGAT AGCCATTTTT 
CAGGTTATAA CCCTAGAAGG ATGGGTTGAT ATCATGTACT ATGTTATGGA CGCACATTCT 
TTTTACAATT TTATATATTT TATACTACTT ATAATAGTGG GTTCTTTCTT CATGATAAAC 
TTGTGCCTGG TTGTGATAGC AACACAGTTT TCTGAAACAA AGCAGCGAGA GAACCAGCTA 
ATGCAGGAGC AACGGGCTCG TTACCTCTCC AACGACAGCA CAATCGCCAG CTTCTCGGAG 
CCAGGGAGCT GCTATGAGGA GCTGCTCAAA TACATCTGCC ACATCTTCAG GAAGGTTAAG 
CGAAGGACGT TAAGACTTTA TAATAACTGG CAGAATAAGA GGAGGAAAAA AGTTCAGCCC 
AACAGCAACA CGAACAGTCA GGGGCGTCAC CGCAAGTGCA AGCGGCGTAT CACCTCCATT 
CATCACTTAA TACACCACCA CCATCATCAC CACCACCATC ATTACCACAT CAGCAATGGG 
AGTCCCCGGG GTCCCAGGTC TAGTCCGGAG ATCTGTGATG TGGAGATGAA AATCATGAAG 
CCAAAGAATC AACTAAGGCT TCCTCCAGCA TCCCCCAGCC TCCAAAGCAT GTCCCCCAAC 
AGTGAGGCCG TCCACAGTAT CTACCACGCG GACTGCCACT TAGAGGCGCC TCAGCGGAAA 
TCGAGCAAGT CTTCCAATGC CTCGTCAAAC ATAAAACTGA ATGCTGGGTT GAATCACAGT 
AACATGAACT ATCCCACCAT CATGCCCTCC CAAGCCAACA AAACCAGCCC CGCAACACTA 
CCCAAAGGAA AAAGGAATGG CAGCAATGCA GTCAGCTTGC TAAGCAGCCC AGTCAGTATG 
AAGGCAGATT CGTTTGGGAA GACCAACCAC CTAGTCGGAG AGCATGGCCT GTGCAGGAGT 
TCCAGTAAGC TCTCCGGTTT GAGCGTGCCA TGCTCCTTGC CAAGCCCTCA GCCAAGCCCT 
CTACTGTGCG AAATCCAGAA CTGCCCGTAC TGTGCCAACC TTTTGGAGAA CCCTGACTTG 
GAGCTCAGCG ACTCTGAGAG TTGCGAATCG GATTCTAACG GGGTCTATGA ATTCACCCAG 
GACTTGAGGC GTGGAGACCA AAGAGACCAG GTGCAGAAAG GCAGAAAGCT CAAGACTAAG 
CCAAAGGAGG CTAACAAGGT GTATCTGCTC TGGGAAGAGT TTACCGTCCG ACTGAAAAGG 
ATTGTGGACA GCAAATATTT TAACCGCGGG ATTATGATTG CCATCCTGAT CAACACGCTG 
AGCATGGGGA TTGAGTATCA TGAGCAGCCA GAAGAACTGA CTAACGCCTT GGAAATCAGC 
AATATTGTGT TTACAAGCAT GTTTGCGCTG GAGATGCTGC TGAAACTCTT GGCCTTTGGG 
ATCTTTCAAT ACATCAAGAA TCCATACAAC ATCTTTGATG GGATCATTGT CGTCATCAGT 
GTGTGGGAAA TCATCGGTCA GTCGGACGGA GGTCTGTCTG TGCTTAGAAC ATTTCGCCTC 
CTTCGTGTGC TGAAGCTGGT ACGATTTATG CCAGCACTGC GGCGTCAGCT GGTGGTCCTG 
GTGAAGACGA TGGACAACGT TGCCACCTTT TGCATGCTTC TTATGCTCTT CATCTTTATA 
TTCAGCATCC TTGGCATGCA TCTGTTTGGG TGTAAGTTTA CTTGGACTGC AGACACCGGA 
GAGACAGTCA AAGACAGAAA GAACTTTGAT TCTTTGCTTT GGGCGATTGT CACCGTCTTC 
CAGATTCTCA CCCAAGAAGA CTGGAACATG GTCCTATACA ATGGCATGGC TTCCACCTCC 
TCCTGGGCAG CTCTCTACTT TGTGGCCCTG ATGACATTTG GAAATTATGT GCTTTTCAAC 
TTGTTAGTAG CCATCTTAGT CGAAGGCTTC CAAGCAGAGG GAGATGCAAC AAGGTCAGAT 
ACAGAGGAGG ACAAGGCTTC AGGCAACTTT GATGAAGATC TGGAGAAACT GAAGGAGCTT 
CGTGCTGCAG AACTGAAGAT GTACTCACTC GCAGTGACAC CCAACGGACA CCTGGACCCT 
CGGAGTTCCA TGCCCCCACC TATCATCATG CGCACCGCCG CCACCCCAAT GCCTACACCA 
AAGAGCTCCC CTTGCATGGA CTCATCACAT CCGTTCGGCG ATTCAAGAAG GGAAAGTAGC 
GTCTCGATAG ATCCATCAAT CGTTGATCAG AAGTCTTTGG CAAGTCTACG CAGCACCCCT 
TGTACCCCCT GGGGCACTTA CAGCAACTGG GGAAGCCGTC GCTCTAGCTG GAATAGTCTG 
GGTAGAGCCC CAAGCATCAA AAAGAAGAGC CAATCAGGGG AGAGGGAGTC CTTACTCTCT 
GGTGAAGGAA AGGGGAGCAC AGATGATGAC TCAGATGACT GCAAGTCAAG CCCAGCGGCG 
AGGGTATCTC TCCATCGCAG GGCAGGTTCC CTGGACACGC GGGGCTCTTT GGATATCCCA 
GATTTCCTGC AGGTGCCCAC GTTACGCCAT TCCCTAAGTG TCAGTCCTTT GGCCATGGTT 
CCTTCAGATT ATCAAGATTG CAATGGGAAA ATGATGCACG TGCCCAGTGA TCTGTTCGTT 
CGGGTGGAGA AACACAGGGA GGATCCGTTG GAGTATGAAG ACGACATAGA AGATAGTTAC 
TGTTTCCGAA TCCGAAAGAT GTTGGAGCCA TACAAACCTA CATGGTGTAA GACGCATGAA 
GACTGGTCCC TCTACATGTT CTCACCTCAA AACCAGTTCC GGATGATGTG CCAAAAAGTC 
ATTGCCCACA AGATGTTTGA CCACGTGGTT CTTGTCTTCA TCTTCTTAAA CTGCATCACC 
ATTGCACTTG AACGGCCTGA TATTGACCCC AATAGCCTGG AACGGATATT CTTGAGTGTC 
TCCAATTACA TTTTTACTGC AATCTTTGTT TTGGAAATGG CAATTAAGGT GGTGGCCCTG 
GGCTTTGTCT CCGGGGAGAA CACTTACCTG CAGAGCAGCT GGAACGTGCT TGATGGGATT 
CTGGTCTTCG TTTCCATCAT CGATATCGTC GTGTCCCTTG CCTCTGCCGG GGGGGCAAAG 
ATCCTGGGAG TCTTGCGGGT ACTGAGGTTG CTGCGGACCC TGCGCCCACT TAGGGTTATC 
AGCCGAGCCC CGGGCCTGAA GTTGGTGGTA GAAACTCTCA TTTCCTCCCT GAGGCCTATC 
GGGAACATTG TCCTTATCTG CTGCGCTTTC TTCATCATCT TTGGCATTCT GGGAGTACAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCGAA GGGTTAGAGA CGAGGAACAT CACCAACAGG 
TCAGAGTGCA CCCGGGCACG GTACAAATGG GTGCGGCGCA AATACAACTT TGACAACCTT 
GGCCAGGCGC TGATGTCGCT CTTTGTCCTC TCCTCCAAAG ATGGCTGGGT GAATATCATG 
TACGATGGCC TGGATGCCGT TGCCACAGAT GTTCAGCCAG TCAAAAACCA CAACCCATGG 
ATGCTCCTGT ACTTCATCTC CTTCCTCCTC ATCGTCAGCT TCTTCGTGCT CAACATGTTC 
GTAGGGGTGG TGGTAGAGAA CTTCCACAAG TGCCGGCAAC ACCAGGAGGC GGAAGAGGCC 
CGGCGCCGGG AGGAGAAACG CCTCCGCCGC CTGGAGAAAA AGCGCAGGAG TAAGGCGTTG 
TATATGTCTG AGGCCCAGCG GAGACCGTAT TACGCAGAGT ACTCGCCGGC GCGCCTCTAC 
ATCCACACCA TGTGTACCAG CCACTACCTG GATCTCTTTA TCACCTGCAT TATTTGTATC 
AACGTCATCA CCATGTCCAT GGAACACTAC AACCAACCCA AGTCCCTGGA TGAAGCTTTA 
AAATACTGCA ATTACGTGTT CACCATCGTC TTCGTGTTCG AGGCCCTGTT CAAGCTTGTG 
GCATTTGGGT TCCGGAGGTT TTTTAAAGAC AGGTGGAACC AACTGGACTT GGCCATTGTT 
TTGCTCTCCA TCATGGGAAT CACGTTAGAG GAGATTGAGA TTAACGCCTC CCTTCCCATC 
AATCCAACTA TTATACGCAT CATGAGGGTG CTGAGGATTG CTCGAGTGTT AAAGCTCCTG 
AAAATGGCCA CCGGGATGCG AGCTTTGCTG GATACTGTTG TGCAAGCGTT ACCTCAGGTA 
GGCAACCTTG GCCTGCTCTT CATGCTCCTG TTCTTCATCT ACGCAGCCCT GGGAGTGGAG 
CTGTTCGGAA AGCTCGTGTG CAATGAGGAG AATCCCTGTG AGGGACTCAG CCGACATGCA 
ACATTTGAGA ACTTTGGGAT GGCTTTTCTG ACCCTCTTCC GTGTTTCCAC AGGGGATAAC 
TGGAATGGCA TCATGAAGGA CACTCTACGA GAGTGCAACA GCGATGACAA GAACTGTCTC 
ACCTACCTGC CCGTCATCTC TCCCGTCTAT TTCGTAACCT TCGTGCTCAT TGCCCAGTTT 
GTCTTGGTCA ATGTGGTCGT GGCTGTGCTC ATGAAACATC TAGAGGAGAG CAATAAAGAG 
GCCAAAGAAG ATGCCGAGAT GGATGCAGAG ATTGAGATGG AGATGTCTAG GGCACTGACA 
CCTATAAGTG GTGGCTGCAC TCCAGCTTCA GATGGCAAGG GAACTGAGGG AGGGCAAGAT 
CCATTAAAGG GGGAGCTGCT GAAACAAGTG GGGGGGGCAG TGTGCCAAGA GAATGTGACC 
TTAGTGATCC CAGAGTCAAT GGAGGGTGCT GCCCTGCTGA ACGACGCAGT CCCTGCTTGC 
TCCATATACC AACAGCAGGC CCTGCCACTT CCCCTCCAGC CTTGCGTCCT TGGGCACGAG 
GATGGGGATT TCCAAAGGAG GGATGGATCG GGCTCCCGAA ACCTACTGGC TGTCAAAAAG 
ATCTCGGTGT CTAGGATGCT CTCCTTACCT AATGACAGCT ACATGTTTCG CCCAGTGCTA 
CCAGCAAAGT CATCTTATCC TTTGCAAGAA GTGGAGACCA CTGAAGATAA TTCACCACAT 
GGGTCGACTG CTTCACTCCA CTCCCAGCCA GGGGACAATA ACTCATTGCT GAATATACCA 
GTGGAGTGCC AGCCACAGGC CTCTGTTAGC AACCACCAGG ACTCTGCCGA CCTGCCTAAA 
ATCCCACCGC CACATACATC CTCAAGATCT CCAAGTCTAA ACAGATCCCT GAGAAGACAG 
GAGGCTATAC GTATGGACTC AGTGGATCAG GGTGGTGATT CTAAGGACCT TAATGTGGAA 
TCTACAGAGA GTTTGCATCT GGGAGCTCCA TCAGTTACTA CTTTAGTTCC AAATTTGCTT 
TGCACCTTAA ATGTGTCTCT GCAGGGAACC CCCCAGTCCT CACCTAGGCT ATCTCCACAG 
CCTAGACATT CCAGTGTCCT CACACACAAC CATGTATACA GCCAACACAG TATTCCAAGT 
CGGCCTTCGA GTGTCAGTAG TGGCAACAGC AACCCAGAGG AATCTATGTT TGAGTCCTCA 
GACCTGGCTG ATGAAGAAGT CAGCCATATA AACAGTTCAG CAAAAGACTG GAGAGGATCA 
TCTAATAATA GAAGTCACTC TGTTTCTCCA TTCATCTCCC AATCTTCATC TCCTCCTCCT 
CTTTCTCCGT CACTAAAGAA CTGCAATAGC ACAGTCAGCC TGGCAGGGTC CAGCGGCAAA 
GATAAAGACT TGAAGAAGTT CTATAGTGTT GATACTCAAG GATTCTTAGC TAAGCCCTGC 
TGGGGGGATG ACCAAAGGAG GCACTCAATT GAGATCTGCC CTTCCATTGA TGATGGAGAT 
TCCTGCTTTG ACCTGTCCAT GGAGGACAAA CATTGCTCTT CTGTTCTCAT TCGGTCTGAC 
CCCGAATACA TCAATGGAGT CCGACGAAAG AAGAAGATGA GTCCCCCTTG CATTTCTATA 
GACCCTCCTA TGGAGGATGA AGGTGGCACA GCTTCCCGTA CCAAAGCATC AGAAAACAGC 
ATGATGTTAC GAAGAAGGAC CCCATCCTGT GAATCTACTG CATACAGGGA CTCTTTAGAT 
CTTAGTGACA ACCAGCTTGG GGACCAAATA TCTAAAACTG ATAGACGAAC CCAGGTGCCC 
TGTCGCAATG AACACTTGAT CATACCCAGC TACTCGTTTG ACAAGGTGGA CGGAAACGGC 
TTCTCAGACC TAATATCTAA CAGTGGGGGT TCTACTCCGA CGTCCGTTTT CAATGAACCT 
GTGTCTTTAG AAATGAAACA AAAAGATCAC AACGATCTGA GCGGCGTTTC TAAAGTTTCG 
TTGAAAACTG CACTAGATCA CGTATCCAAC CCAACAGAGG ACATAATCGA TAACCCATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1h ( XM_031892924.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748784.1 Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X2, mRNA.

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CloneID	OXa66327
Clone ID Related Accession (Same CDS sequence)	XM_031892924.1
Accession Version	XM_031892924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGATG GCGAGAGGAA AGAGCCCTTC CAGCCACTGG CCGGAGACGA TATCCGGGTG 
CCCATACCCC CGACACCCCA AGCAGGGGAC CCCAGTGAAG AAGAGGATGC GGGAAATGCA 
GAGAAGTCAG CTCTGGGCAG CTTGGGCTCT GGAGGTTCTT CTGGAAGAGG TCGTGAACAA 
GGGATGGGCT CGGAGGAAGG AGAACAGGTT CCATACCCAG CCCTGGCACC CACCGTCTTC 
TTCTGTCTCA GCCAAACCAC GCGTCCACGG AACTGGTGCC TCAGACTGGT CTGTAATCCA 
TGGTTCGAGC ATGTCAGCAT GCTGGTCATT TTGCTGAACT GCGTCACCCT GGGGATGTTC 
CAGCCCTGCG AGGATACAAA CTGTATGTCA GAGAGATGCA ACATTCTAGA GGCATTCGAC 
GGCTTCATCT TTGCATTCTT TGCTGTGGAG ATGGTTATTA AGATGGTAGC GCTTGGCATC 
TTTGGGCAGA AGTGCTACCT GGGAGACACA TGGAACTGGC TGGATTTCTT CATTGTTATG 
GCAGGGATGT TGGAATATTC CCTCGATGGA CACAATGTCA GCCTATCCGC TATCCGCACT 
GTACGTGTTT TAAGGCCGCT AAGAGCCATT AACAGGGTTC CCAGTATGAG GATACTGGTG 
ACCCTGCTGC TAGATACTCT GCCTATGTTA GGCAACGTCC TTCTCTTGTG TTTCTTTGTG 
TTTTTCATCT TTGGAATCGT CGGCGTTCAG CTTTGGGCTG GGCTACTGCG CAACAGGTGT 
TTTATGGATA CAAATTTTGT AATGAGTTAC AATTTCACCC CTCTGAGGCC GTATTACCAG 
GTGGATGATG CAGAGGACAA CCCTTATATC TGCTCCTCTA ACCGAGACAA TGGGATGTTA 
AAGTGCCAGG ACATCCCTCC CAAAATGGAC TGCTCCCATG TATACTACTC AGATTTCCCC 
AGTAGGAATA GCTGCGTGAA TATTAATCAA TACTACAACG TATGCAAGCC GGGAGAGTTC 
AATCCACATA AAGGGGCCAT TAATTTTGAT AACATTGGCT ACGCCTGGAT AGCCATTTTT 
CAGGTTATAA CCCTAGAAGG ATGGGTTGAT ATCATGTACT ATGTTATGGA CGCACATTCT 
TTTTACAATT TTATATATTT TATACTACTT ATAATAGTGG GTTCTTTCTT CATGATAAAC 
TTGTGCCTGG TTGTGATAGC AACACAGTTT TCTGAAACAA AGCAGCGAGA GAACCAGCTA 
ATGCAGGAGC AACGGGCTCG TTACCTCTCC AACGACAGCA CAATCGCCAG CTTCTCGGAG 
CCAGGGAGCT GCTATGAGGA GCTGCTCAAA TACATCTGCC ACATCTTCAG GAAGGTTAAG 
CGAAGGACGT TAAGACTTTA TAATAACTGG CAGAATAAGA GGAGGAAAAA AGTTCAGCCC 
AACAGCAACA CGAACAGTCA GGGGCGTCAC CGCAAGTGCA AGCGGCGTAT CACCTCCATT 
CATCACTTAA TACACCACCA CCATCATCAC CACCACCATC ATTACCACAT CAGCAATGGG 
AGTCCCCGGG GTCCCAGGTC TAGTCCGGAG ATCTGTGATG TGGAGATGAA AATCATGAAG 
CCAAAGAATC AACTAAGGCT TCCTCCAGCA TCCCCCAGCC TCCAAAGCAT GTCCCCCAAC 
AGTGAGGCCG TCCACAGTAT CTACCACGCG GACTGCCACT TAGAGGCGCC TCAGCGGAAA 
TCGAGCAAGT CTTCCAATGC CTCGTCAAAC ATAAAACTGA ATGCTGGGTT GAATCACAGT 
AACATGAACT ATCCCACCAT CATGCCCTCC CAAGCCAACA AAACCAGCCC CGCAACACTA 
CCCAAAGGAA AAAGGAATGG CAGCAATGCA GTCAGCTTGC TAAGCAGCCC AGTCAGTATG 
AAGGCAGATT CGTTTGGGAA GACCAACCAC CTAGTCGGAG AGCATGGCCT GTGCAGGAGT 
TCCAGTAAGC TCTCCGGTTT GAGCGTGCCA TGCTCCTTGC CAAGCCCTCA GCCAAGCCCT 
CTACTGTGCG AAATCCAGAA CTGCCCGTAC TGTGCCAACC TTTTGGAGAA CCCTGACTTG 
GAGCTCAGCG ACTCTGAGAG TTGCGAATCG GATTCTAACG GGGTCTATGA ATTCACCCAG 
GACTTGAGGC GTGGAGACCA AAGAGACCAG GTGCAGAAAG GCAGAAAGCT CAAGACTAAG 
CCAAAGGAGG CTAACAAGGT GTATCTGCTC TGGGAAGAGT TTACCGTCCG ACTGAAAAGG 
ATTGTGGACA GCAAATATTT TAACCGCGGG ATTATGATTG CCATCCTGAT CAACACGCTG 
AGCATGGGGA TTGAGTATCA TGAGCAGCCA GAAGAACTGA CTAACGCCTT GGAAATCAGC 
AATATTGTGT TTACAAGCAT GTTTGCGCTG GAGATGCTGC TGAAACTCTT GGCCTTTGGG 
ATCTTTCAAT ACATCAAGAA TCCATACAAC ATCTTTGATG GGATCATTGT CGTCATCAGT 
GTGTGGGAAA TCATCGGTCA GTCGGACGGA GGTCTGTCTG TGCTTAGAAC ATTTCGCCTC 
CTTCGTGTGC TGAAGCTGGT ACGATTTATG CCAGCACTGC GGCGTCAGCT GGTGGTCCTG 
GTGAAGACGA TGGACAACGT TGCCACCTTT TGCATGCTTC TTATGCTCTT CATCTTTATA 
TTCAGCATCC TTGGCATGCA TCTGTTTGGG TGTAAGTTTA CTTGGACTGC AGACACCGGA 
GAGACAGTCA AAGACAGAAA GAACTTTGAT TCTTTGCTTT GGGCGATTGT CACCGTCTTC 
CAGATTCTCA CCCAAGAAGA CTGGAACATG GTCCTATACA ATGGCATGGC TTCCACCTCC 
TCCTGGGCAG CTCTCTACTT TGTGGCCCTG ATGACATTTG GAAATTATGT GCTTTTCAAC 
TTGTTAGTAG CCATCTTAGT CGAAGGCTTC CAAGCAGAGG GAGATGCAAC AAGGTCAGAT 
ACAGAGGAGG ACAAGGCTTC AGGCAACTTT GATGAAGATC TGGAGAAACT GAAGGAGCTT 
CGTGCTGCAG AACTGAAGAT GTACTCACTC GCAGTGACAC CCAACGGACA CCTGGACCCT 
CGGAGTTCCA TGCCCCCACC TATCATCATG CGCACCGCCG CCACCCCAAT GCCTACACCA 
AAGAGCTCCC CTTGCATGGA CTCATCACAT CCGTTCGGCG ATTCAAGAAG GGAAAGTAGC 
GTCTCGATAG ATCCATCAAT CGTTGATCAG AAGTCTTTGG CAAGTCTACG CAGCACCCCT 
TGTACCCCCT GGGGCACTTA CAGCAACTGG GGAAGCCGTC GCTCTAGCTG GAATAGTCTG 
GGTAGAGCCC CAAGCATCAA AAAGAAGAGC CAATCAGGGG AGAGGGAGTC CTTACTCTCT 
GGTGAAGGAA AGGGGAGCAC AGATGATGAC TCAGATGACT GCAAGTCAAG CCCAGCGGCG 
AGGGTATCTC TCCATCGCAG GGCAGGTTCC CTGGACACGC GGGGCTCTTT GGATATCCCA 
GATTTCCTGC AGGTGCCCAC GTTACGCCAT TCCCTAAGTG TCAGTCCTTT GGCCATGGTT 
CCTTCAGATT ATCAAGATTG CAATGGGAAA ATGATGCACG TGCCCAGTGA TCTGTTCGTT 
CGGGTGGAGA AACACAGGGA GGATCCGTTG GAGTATGAAG ACGACATAGA AGATAGTTAC 
TGTTTCCGAA TCCGAAAGAT GTTGGAGCCA TACAAACCTA CATGGTGTAA GACGCATGAA 
GACTGGTCCC TCTACATGTT CTCACCTCAA AACCAGTTCC GGATGATGTG CCAAAAAGTC 
ATTGCCCACA AGATGTTTGA CCACGTGGTT CTTGTCTTCA TCTTCTTAAA CTGCATCACC 
ATTGCACTTG AACGGCCTGA TATTGACCCC AATAGCCTGG AACGGATATT CTTGAGTGTC 
TCCAATTACA TTTTTACTGC AATCTTTGTT TTGGAAATGG CAATTAAGGT GGTGGCCCTG 
GGCTTTGTCT CCGGGGAGAA CACTTACCTG CAGAGCAGCT GGAACGTGCT TGATGGGATT 
CTGGTCTTCG TTTCCATCAT CGATATCGTC GTGTCCCTTG CCTCTGCCGG GGGGGCAAAG 
ATCCTGGGAG TCTTGCGGGT ACTGAGGTTG CTGCGGACCC TGCGCCCACT TAGGGTTATC 
AGCCGAGCCC CGGGCCTGAA GTTGGTGGTA GAAACTCTCA TTTCCTCCCT GAGGCCTATC 
GGGAACATTG TCCTTATCTG CTGCGCTTTC TTCATCATCT TTGGCATTCT GGGAGTACAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCGAA GGGTTAGAGA CGAGGAACAT CACCAACAGG 
TCAGAGTGCA CCCGGGCACG GTACAAATGG GTGCGGCGCA AATACAACTT TGACAACCTT 
GGCCAGGCGC TGATGTCGCT CTTTGTCCTC TCCTCCAAAG ATGGCTGGGT GAATATCATG 
TACGATGGCC TGGATGCCGT TGCCACAGAT GTTCAGCCAG TCAAAAACCA CAACCCATGG 
ATGCTCCTGT ACTTCATCTC CTTCCTCCTC ATCGTCAGCT TCTTCGTGCT CAACATGTTC 
GTAGGGGTGG TGGTAGAGAA CTTCCACAAG TGCCGGCAAC ACCAGGAGGC GGAAGAGGCC 
CGGCGCCGGG AGGAGAAACG CCTCCGCCGC CTGGAGAAAA AGCGCAGGAA GGCCCAGCGG 
AGACCGTATT ACGCAGAGTA CTCGCCGGCG CGCCTCTACA TCCACACCAT GTGTACCAGC 
CACTACCTGG ATCTCTTTAT CACCTGCATT ATTTGTATCA ACGTCATCAC CATGTCCATG 
GAACACTACA ACCAACCCAA GTCCCTGGAT GAAGCTTTAA AATACTGCAA TTACGTGTTC 
ACCATCGTCT TCGTGTTCGA GGCCCTGTTC AAGCTTGTGG CATTTGGGTT CCGGAGGTTT 
TTTAAAGACA GGTGGAACCA ACTGGACTTG GCCATTGTTT TGCTCTCCAT CATGGGAATC 
ACGTTAGAGG AGATTGAGAT TAACGCCTCC CTTCCCATCA ATCCAACTAT TATACGCATC 
ATGAGGGTGC TGAGGATTGC TCGAGTGTTA AAGCTCCTGA AAATGGCCAC CGGGATGCGA 
GCTTTGCTGG ATACTGTTGT GCAAGCGTTA CCTCAGGTAG GCAACCTTGG CCTGCTCTTC 
ATGCTCCTGT TCTTCATCTA CGCAGCCCTG GGAGTGGAGC TGTTCGGAAA GCTCGTGTGC 
AATGAGGAGA ATCCCTGTGA GGGACTCAGC CGACATGCAA CATTTGAGAA CTTTGGGATG 
GCTTTTCTGA CCCTCTTCCG TGTTTCCACA GGGGATAACT GGAATGGCAT CATGAAGGAC 
ACTCTACGAG AGTGCAACAG CGATGACAAG AACTGTCTCA CCTACCTGCC CGTCATCTCT 
CCCGTCTATT TCGTAACCTT CGTGCTCATT GCCCAGTTTG TCTTGGTCAA TGTGGTCGTG 
GCTGTGCTCA TGAAACATCT AGAGGAGAGC AATAAAGAGG CCAAAGAAGA TGCCGAGATG 
GATGCAGAGA TTGAGATGGA GATGTCTAGG GCACTGACAC CTATAAGTGG TGGCTGCACT 
CCAGCTTCAG ATGGCAAGGG AACTGAGGGA GGGCAAGATC CATTAAAGGG GGAGCTGCTG 
AAACAAGTGG GGGGGGCAGT GTGCCAAGAG AATGTGACCT TAGTGATCCC AGAGTCAATG 
GAGGGTGCTG CCCTGCTGAA CGACGCAGTC CCTGCTTGCT CCATATACCA ACAGCAGGCC 
CTGCCACTTC CCCTCCAGCC TTGCGTCCTT GGGCACGAGG ATGGGGATTT CCAAAGGAGG 
GATGGATCGG GCTCCCGAAA CCTACTGGCT GTCAAAAAGA TCTCGGTGTC TAGGATGCTC 
TCCTTACCTA ATGACAGCTA CATGTTTCGC CCAGTGCTAC CAGCAAAGTC ATCTTATCCT 
TTGCAAGAAG TGGAGACCAC TGAAGATAAT TCACCACATG GGTCGACTGC TTCACTCCAC 
TCCCAGCCAG GGGACAATAA CTCATTGCTG AATATACCAG TGGAGTGCCA GCCACAGGCC 
TCTGTTAGCA ACCACCAGGA CTCTGCCGAC CTGCCTAAAA TCCCACCGCC ACATACATCC 
TCAAGATCTC CAAGTCTAAA CAGATCCCTG AGAAGACAGG AGGCTATACG TATGGACTCA 
GTGGATCAGG GTGGTGATTC TAAGGACCTT AATGTGGAAT CTACAGAGAG TTTGCATCTG 
GGAGCTCCAT CAGTTACTAC TTTAGTTCCA AATTTGCTTT GCACCTTAAA TGTGTCTCTG 
CAGGGAACCC CCCAGTCCTC ACCTAGGCTA TCTCCACAGC CTAGACATTC CAGTGTCCTC 
ACACACAACC ATGTATACAG CCAACACAGT ATTCCAAGTC GGCCTTCGAG TGTCAGTAGT 
GGCAACAGCA ACCCAGAGGA ATCTATGTTT GAGTCCTCAG ACCTGGCTGA TGAAGAAGTC 
AGCCATATAA ACAGTTCAGC AAAAGACTGG AGAGGATCAT CTAATAATAG AAGTCACTCT 
GTTTCTCCAT TCATCTCCCA ATCTTCATCT CCTCCTCCTC TTTCTCCGTC ACTAAAGAAC 
TGCAATAGCA CAGTCAGCCT GGCAGGGTCC AGCGGCAAAG ATAAAGACTT GAAGAAGTTC 
TATAGTGTTG ATACTCAAGG ATTCTTAGCT AAGCCCTGCT GGGGGGATGA CCAAAGGAGG 
CACTCAATTG AGATCTGCCC TTCCATTGAT GATGGAGATT CCTGCTTTGA CCTGTCCATG 
GAGGACAAAC ATTGCTCTTC TGTTCTCATT CGGTCTGACC CCGAATACAT CAATGGAGTC 
CGACGAAAGA AGAAGATGAG TCCCCCTTGC ATTTCTATAG ACCCTCCTAT GGAGGATGAA 
GGTGGCACAG CTTCCCGTAC CAAAGCATCA GAAAACAGCA TGATGTTACG AAGAAGGACC 
CCATCCTGTG AATCTACTGC ATACAGGGAC TCTTTAGATC TTAGTGACAA CCAGCTTGGG 
GACCAAATAT CTAAAACTGA TAGACGAACC CAGGTGCCCT GTCGCAATGA ACACTTGATC 
ATACCCAGCT ACTCGTTTGA CAAGGTGGAC GGAAACGGCT TCTCAGACCT AATATCTAAC 
AGTGGGGGTT CTACTCCGAC GTCCGTTTTC AATGAACCTG TGTCTTTAGA AATGAAACAA 
AAAGATCACA ACGATCTGAG CGGCGTTTCT AAAGTTTCGT TGAAAACTGC ACTAGATCAC 
GTATCCAACC CAACAGAGGA CATAATCGAT AACCCATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748784.1
CDS	448..7809
Translation	MTDGERKEPFQPLAGDDIRVPIPPTPQAGDPSEEEDAGNAEKSALGSLGSGGSSGRGREQGMGSEEGEQVPYPALAPTVFFCLSQTTRPRNWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKCKRRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSSPEICDVEMKIMKPKNQLRLPPASPSLQSMSPNSEAVHSIYHADCHLEAPQRKSSKSSNASSNIKLNAGLNHSNMNYPTIMPSQANKTSPATLPKGKRNGSNAVSLLSSPVSMKADSFGKTNHLVGEHGLCRSSSKLSGLSVPCSLPSPQPSPLLCEIQNCPYCANLLENPDLELSDSESCESDSNGVYEFTQDLRRGDQRDQVQKGRKLKTKPKEANKVYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTEEDKASGNFDEDLEKLKELRAAELKMYSLAVTPNGHLDPRSSMPPPIIMRTAATPMPTPKSSPCMDSSHPFGDSRRESSVSIDPSIVDQKSLASLRSTPCTPWGTYSNWGSRRSSWNSLGRAPSIKKKSQSGERESLLSGEGKGSTDDDSDDCKSSPAARVSLHRRAGSLDTRGSLDIPDFLQVPTLRHSLSVSPLAMVPSDYQDCNGKMMHVPSDLFVRVEKHREDPLEYEDDIEDSYCFRIRKMLEPYKPTWCKTHEDWSLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRRPYYAEYSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNSDDKNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEMSRALTPISGGCTPASDGKGTEGGQDPLKGELLKQVGGAVCQENVTLVIPESMEGAALLNDAVPACSIYQQQALPLPLQPCVLGHEDGDFQRRDGSGSRNLLAVKKISVSRMLSLPNDSYMFRPVLPAKSSYPLQEVETTEDNSPHGSTASLHSQPGDNNSLLNIPVECQPQASVSNHQDSADLPKIPPPHTSSRSPSLNRSLRRQEAIRMDSVDQGGDSKDLNVESTESLHLGAPSVTTLVPNLLCTLNVSLQGTPQSSPRLSPQPRHSSVLTHNHVYSQHSIPSRPSSVSSGNSNPEESMFESSDLADEEVSHINSSAKDWRGSSNNRSHSVSPFISQSSSPPPLSPSLKNCNSTVSLAGSSGKDKDLKKFYSVDTQGFLAKPCWGDDQRRHSIEICPSIDDGDSCFDLSMEDKHCSSVLIRSDPEYINGVRRKKKMSPPCISIDPPMEDEGGTASRTKASENSMMLRRRTPSCESTAYRDSLDLSDNQLGDQISKTDRRTQVPCRNEHLIIPSYSFDKVDGNGFSDLISNSGGSTPTSVFNEPVSLEMKQKDHNDLSGVSKVSLKTALDHVSNPTEDIIDNPL

Target ORF information:

RefSeq Version	XM_031892924.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892924.1

ORF Insert Sequence:

ATGACTGATG GCGAGAGGAA AGAGCCCTTC CAGCCACTGG CCGGAGACGA TATCCGGGTG 
CCCATACCCC CGACACCCCA AGCAGGGGAC CCCAGTGAAG AAGAGGATGC GGGAAATGCA 
GAGAAGTCAG CTCTGGGCAG CTTGGGCTCT GGAGGTTCTT CTGGAAGAGG TCGTGAACAA 
GGGATGGGCT CGGAGGAAGG AGAACAGGTT CCATACCCAG CCCTGGCACC CACCGTCTTC 
TTCTGTCTCA GCCAAACCAC GCGTCCACGG AACTGGTGCC TCAGACTGGT CTGTAATCCA 
TGGTTCGAGC ATGTCAGCAT GCTGGTCATT TTGCTGAACT GCGTCACCCT GGGGATGTTC 
CAGCCCTGCG AGGATACAAA CTGTATGTCA GAGAGATGCA ACATTCTAGA GGCATTCGAC 
GGCTTCATCT TTGCATTCTT TGCTGTGGAG ATGGTTATTA AGATGGTAGC GCTTGGCATC 
TTTGGGCAGA AGTGCTACCT GGGAGACACA TGGAACTGGC TGGATTTCTT CATTGTTATG 
GCAGGGATGT TGGAATATTC CCTCGATGGA CACAATGTCA GCCTATCCGC TATCCGCACT 
GTACGTGTTT TAAGGCCGCT AAGAGCCATT AACAGGGTTC CCAGTATGAG GATACTGGTG 
ACCCTGCTGC TAGATACTCT GCCTATGTTA GGCAACGTCC TTCTCTTGTG TTTCTTTGTG 
TTTTTCATCT TTGGAATCGT CGGCGTTCAG CTTTGGGCTG GGCTACTGCG CAACAGGTGT 
TTTATGGATA CAAATTTTGT AATGAGTTAC AATTTCACCC CTCTGAGGCC GTATTACCAG 
GTGGATGATG CAGAGGACAA CCCTTATATC TGCTCCTCTA ACCGAGACAA TGGGATGTTA 
AAGTGCCAGG ACATCCCTCC CAAAATGGAC TGCTCCCATG TATACTACTC AGATTTCCCC 
AGTAGGAATA GCTGCGTGAA TATTAATCAA TACTACAACG TATGCAAGCC GGGAGAGTTC 
AATCCACATA AAGGGGCCAT TAATTTTGAT AACATTGGCT ACGCCTGGAT AGCCATTTTT 
CAGGTTATAA CCCTAGAAGG ATGGGTTGAT ATCATGTACT ATGTTATGGA CGCACATTCT 
TTTTACAATT TTATATATTT TATACTACTT ATAATAGTGG GTTCTTTCTT CATGATAAAC 
TTGTGCCTGG TTGTGATAGC AACACAGTTT TCTGAAACAA AGCAGCGAGA GAACCAGCTA 
ATGCAGGAGC AACGGGCTCG TTACCTCTCC AACGACAGCA CAATCGCCAG CTTCTCGGAG 
CCAGGGAGCT GCTATGAGGA GCTGCTCAAA TACATCTGCC ACATCTTCAG GAAGGTTAAG 
CGAAGGACGT TAAGACTTTA TAATAACTGG CAGAATAAGA GGAGGAAAAA AGTTCAGCCC 
AACAGCAACA CGAACAGTCA GGGGCGTCAC CGCAAGTGCA AGCGGCGTAT CACCTCCATT 
CATCACTTAA TACACCACCA CCATCATCAC CACCACCATC ATTACCACAT CAGCAATGGG 
AGTCCCCGGG GTCCCAGGTC TAGTCCGGAG ATCTGTGATG TGGAGATGAA AATCATGAAG 
CCAAAGAATC AACTAAGGCT TCCTCCAGCA TCCCCCAGCC TCCAAAGCAT GTCCCCCAAC 
AGTGAGGCCG TCCACAGTAT CTACCACGCG GACTGCCACT TAGAGGCGCC TCAGCGGAAA 
TCGAGCAAGT CTTCCAATGC CTCGTCAAAC ATAAAACTGA ATGCTGGGTT GAATCACAGT 
AACATGAACT ATCCCACCAT CATGCCCTCC CAAGCCAACA AAACCAGCCC CGCAACACTA 
CCCAAAGGAA AAAGGAATGG CAGCAATGCA GTCAGCTTGC TAAGCAGCCC AGTCAGTATG 
AAGGCAGATT CGTTTGGGAA GACCAACCAC CTAGTCGGAG AGCATGGCCT GTGCAGGAGT 
TCCAGTAAGC TCTCCGGTTT GAGCGTGCCA TGCTCCTTGC CAAGCCCTCA GCCAAGCCCT 
CTACTGTGCG AAATCCAGAA CTGCCCGTAC TGTGCCAACC TTTTGGAGAA CCCTGACTTG 
GAGCTCAGCG ACTCTGAGAG TTGCGAATCG GATTCTAACG GGGTCTATGA ATTCACCCAG 
GACTTGAGGC GTGGAGACCA AAGAGACCAG GTGCAGAAAG GCAGAAAGCT CAAGACTAAG 
CCAAAGGAGG CTAACAAGGT GTATCTGCTC TGGGAAGAGT TTACCGTCCG ACTGAAAAGG 
ATTGTGGACA GCAAATATTT TAACCGCGGG ATTATGATTG CCATCCTGAT CAACACGCTG 
AGCATGGGGA TTGAGTATCA TGAGCAGCCA GAAGAACTGA CTAACGCCTT GGAAATCAGC 
AATATTGTGT TTACAAGCAT GTTTGCGCTG GAGATGCTGC TGAAACTCTT GGCCTTTGGG 
ATCTTTCAAT ACATCAAGAA TCCATACAAC ATCTTTGATG GGATCATTGT CGTCATCAGT 
GTGTGGGAAA TCATCGGTCA GTCGGACGGA GGTCTGTCTG TGCTTAGAAC ATTTCGCCTC 
CTTCGTGTGC TGAAGCTGGT ACGATTTATG CCAGCACTGC GGCGTCAGCT GGTGGTCCTG 
GTGAAGACGA TGGACAACGT TGCCACCTTT TGCATGCTTC TTATGCTCTT CATCTTTATA 
TTCAGCATCC TTGGCATGCA TCTGTTTGGG TGTAAGTTTA CTTGGACTGC AGACACCGGA 
GAGACAGTCA AAGACAGAAA GAACTTTGAT TCTTTGCTTT GGGCGATTGT CACCGTCTTC 
CAGATTCTCA CCCAAGAAGA CTGGAACATG GTCCTATACA ATGGCATGGC TTCCACCTCC 
TCCTGGGCAG CTCTCTACTT TGTGGCCCTG ATGACATTTG GAAATTATGT GCTTTTCAAC 
TTGTTAGTAG CCATCTTAGT CGAAGGCTTC CAAGCAGAGG GAGATGCAAC AAGGTCAGAT 
ACAGAGGAGG ACAAGGCTTC AGGCAACTTT GATGAAGATC TGGAGAAACT GAAGGAGCTT 
CGTGCTGCAG AACTGAAGAT GTACTCACTC GCAGTGACAC CCAACGGACA CCTGGACCCT 
CGGAGTTCCA TGCCCCCACC TATCATCATG CGCACCGCCG CCACCCCAAT GCCTACACCA 
AAGAGCTCCC CTTGCATGGA CTCATCACAT CCGTTCGGCG ATTCAAGAAG GGAAAGTAGC 
GTCTCGATAG ATCCATCAAT CGTTGATCAG AAGTCTTTGG CAAGTCTACG CAGCACCCCT 
TGTACCCCCT GGGGCACTTA CAGCAACTGG GGAAGCCGTC GCTCTAGCTG GAATAGTCTG 
GGTAGAGCCC CAAGCATCAA AAAGAAGAGC CAATCAGGGG AGAGGGAGTC CTTACTCTCT 
GGTGAAGGAA AGGGGAGCAC AGATGATGAC TCAGATGACT GCAAGTCAAG CCCAGCGGCG 
AGGGTATCTC TCCATCGCAG GGCAGGTTCC CTGGACACGC GGGGCTCTTT GGATATCCCA 
GATTTCCTGC AGGTGCCCAC GTTACGCCAT TCCCTAAGTG TCAGTCCTTT GGCCATGGTT 
CCTTCAGATT ATCAAGATTG CAATGGGAAA ATGATGCACG TGCCCAGTGA TCTGTTCGTT 
CGGGTGGAGA AACACAGGGA GGATCCGTTG GAGTATGAAG ACGACATAGA AGATAGTTAC 
TGTTTCCGAA TCCGAAAGAT GTTGGAGCCA TACAAACCTA CATGGTGTAA GACGCATGAA 
GACTGGTCCC TCTACATGTT CTCACCTCAA AACCAGTTCC GGATGATGTG CCAAAAAGTC 
ATTGCCCACA AGATGTTTGA CCACGTGGTT CTTGTCTTCA TCTTCTTAAA CTGCATCACC 
ATTGCACTTG AACGGCCTGA TATTGACCCC AATAGCCTGG AACGGATATT CTTGAGTGTC 
TCCAATTACA TTTTTACTGC AATCTTTGTT TTGGAAATGG CAATTAAGGT GGTGGCCCTG 
GGCTTTGTCT CCGGGGAGAA CACTTACCTG CAGAGCAGCT GGAACGTGCT TGATGGGATT 
CTGGTCTTCG TTTCCATCAT CGATATCGTC GTGTCCCTTG CCTCTGCCGG GGGGGCAAAG 
ATCCTGGGAG TCTTGCGGGT ACTGAGGTTG CTGCGGACCC TGCGCCCACT TAGGGTTATC 
AGCCGAGCCC CGGGCCTGAA GTTGGTGGTA GAAACTCTCA TTTCCTCCCT GAGGCCTATC 
GGGAACATTG TCCTTATCTG CTGCGCTTTC TTCATCATCT TTGGCATTCT GGGAGTACAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCGAA GGGTTAGAGA CGAGGAACAT CACCAACAGG 
TCAGAGTGCA CCCGGGCACG GTACAAATGG GTGCGGCGCA AATACAACTT TGACAACCTT 
GGCCAGGCGC TGATGTCGCT CTTTGTCCTC TCCTCCAAAG ATGGCTGGGT GAATATCATG 
TACGATGGCC TGGATGCCGT TGCCACAGAT GTTCAGCCAG TCAAAAACCA CAACCCATGG 
ATGCTCCTGT ACTTCATCTC CTTCCTCCTC ATCGTCAGCT TCTTCGTGCT CAACATGTTC 
GTAGGGGTGG TGGTAGAGAA CTTCCACAAG TGCCGGCAAC ACCAGGAGGC GGAAGAGGCC 
CGGCGCCGGG AGGAGAAACG CCTCCGCCGC CTGGAGAAAA AGCGCAGGAA GGCCCAGCGG 
AGACCGTATT ACGCAGAGTA CTCGCCGGCG CGCCTCTACA TCCACACCAT GTGTACCAGC 
CACTACCTGG ATCTCTTTAT CACCTGCATT ATTTGTATCA ACGTCATCAC CATGTCCATG 
GAACACTACA ACCAACCCAA GTCCCTGGAT GAAGCTTTAA AATACTGCAA TTACGTGTTC 
ACCATCGTCT TCGTGTTCGA GGCCCTGTTC AAGCTTGTGG CATTTGGGTT CCGGAGGTTT 
TTTAAAGACA GGTGGAACCA ACTGGACTTG GCCATTGTTT TGCTCTCCAT CATGGGAATC 
ACGTTAGAGG AGATTGAGAT TAACGCCTCC CTTCCCATCA ATCCAACTAT TATACGCATC 
ATGAGGGTGC TGAGGATTGC TCGAGTGTTA AAGCTCCTGA AAATGGCCAC CGGGATGCGA 
GCTTTGCTGG ATACTGTTGT GCAAGCGTTA CCTCAGGTAG GCAACCTTGG CCTGCTCTTC 
ATGCTCCTGT TCTTCATCTA CGCAGCCCTG GGAGTGGAGC TGTTCGGAAA GCTCGTGTGC 
AATGAGGAGA ATCCCTGTGA GGGACTCAGC CGACATGCAA CATTTGAGAA CTTTGGGATG 
GCTTTTCTGA CCCTCTTCCG TGTTTCCACA GGGGATAACT GGAATGGCAT CATGAAGGAC 
ACTCTACGAG AGTGCAACAG CGATGACAAG AACTGTCTCA CCTACCTGCC CGTCATCTCT 
CCCGTCTATT TCGTAACCTT CGTGCTCATT GCCCAGTTTG TCTTGGTCAA TGTGGTCGTG 
GCTGTGCTCA TGAAACATCT AGAGGAGAGC AATAAAGAGG CCAAAGAAGA TGCCGAGATG 
GATGCAGAGA TTGAGATGGA GATGTCTAGG GCACTGACAC CTATAAGTGG TGGCTGCACT 
CCAGCTTCAG ATGGCAAGGG AACTGAGGGA GGGCAAGATC CATTAAAGGG GGAGCTGCTG 
AAACAAGTGG GGGGGGCAGT GTGCCAAGAG AATGTGACCT TAGTGATCCC AGAGTCAATG 
GAGGGTGCTG CCCTGCTGAA CGACGCAGTC CCTGCTTGCT CCATATACCA ACAGCAGGCC 
CTGCCACTTC CCCTCCAGCC TTGCGTCCTT GGGCACGAGG ATGGGGATTT CCAAAGGAGG 
GATGGATCGG GCTCCCGAAA CCTACTGGCT GTCAAAAAGA TCTCGGTGTC TAGGATGCTC 
TCCTTACCTA ATGACAGCTA CATGTTTCGC CCAGTGCTAC CAGCAAAGTC ATCTTATCCT 
TTGCAAGAAG TGGAGACCAC TGAAGATAAT TCACCACATG GGTCGACTGC TTCACTCCAC 
TCCCAGCCAG GGGACAATAA CTCATTGCTG AATATACCAG TGGAGTGCCA GCCACAGGCC 
TCTGTTAGCA ACCACCAGGA CTCTGCCGAC CTGCCTAAAA TCCCACCGCC ACATACATCC 
TCAAGATCTC CAAGTCTAAA CAGATCCCTG AGAAGACAGG AGGCTATACG TATGGACTCA 
GTGGATCAGG GTGGTGATTC TAAGGACCTT AATGTGGAAT CTACAGAGAG TTTGCATCTG 
GGAGCTCCAT CAGTTACTAC TTTAGTTCCA AATTTGCTTT GCACCTTAAA TGTGTCTCTG 
CAGGGAACCC CCCAGTCCTC ACCTAGGCTA TCTCCACAGC CTAGACATTC CAGTGTCCTC 
ACACACAACC ATGTATACAG CCAACACAGT ATTCCAAGTC GGCCTTCGAG TGTCAGTAGT 
GGCAACAGCA ACCCAGAGGA ATCTATGTTT GAGTCCTCAG ACCTGGCTGA TGAAGAAGTC 
AGCCATATAA ACAGTTCAGC AAAAGACTGG AGAGGATCAT CTAATAATAG AAGTCACTCT 
GTTTCTCCAT TCATCTCCCA ATCTTCATCT CCTCCTCCTC TTTCTCCGTC ACTAAAGAAC 
TGCAATAGCA CAGTCAGCCT GGCAGGGTCC AGCGGCAAAG ATAAAGACTT GAAGAAGTTC 
TATAGTGTTG ATACTCAAGG ATTCTTAGCT AAGCCCTGCT GGGGGGATGA CCAAAGGAGG 
CACTCAATTG AGATCTGCCC TTCCATTGAT GATGGAGATT CCTGCTTTGA CCTGTCCATG 
GAGGACAAAC ATTGCTCTTC TGTTCTCATT CGGTCTGACC CCGAATACAT CAATGGAGTC 
CGACGAAAGA AGAAGATGAG TCCCCCTTGC ATTTCTATAG ACCCTCCTAT GGAGGATGAA 
GGTGGCACAG CTTCCCGTAC CAAAGCATCA GAAAACAGCA TGATGTTACG AAGAAGGACC 
CCATCCTGTG AATCTACTGC ATACAGGGAC TCTTTAGATC TTAGTGACAA CCAGCTTGGG 
GACCAAATAT CTAAAACTGA TAGACGAACC CAGGTGCCCT GTCGCAATGA ACACTTGATC 
ATACCCAGCT ACTCGTTTGA CAAGGTGGAC GGAAACGGCT TCTCAGACCT AATATCTAAC 
AGTGGGGGTT CTACTCCGAC GTCCGTTTTC AATGAACCTG TGTCTTTAGA AATGAAACAA 
AAAGATCACA ACGATCTGAG CGGCGTTTCT AAAGTTTCGT TGAAAACTGC ACTAGATCAC 
GTATCCAACC CAACAGAGGA CATAATCGAT AACCCATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1h ( XM_031892925.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748785.1 Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X3, mRNA.

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CloneID	OXa66328
Clone ID Related Accession (Same CDS sequence)	XM_031892925.1
Accession Version	XM_031892925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGATG GCGAGAGGAA AGAGCCCTTC CAGCCACTGG CCGGAGACGA TATCCGGGTG 
CCCATACCCC CGACACCCCA AGCAGGGGAC CCCAGTGAAG AAGAGGATGC GGGAAATGCA 
GAGAAGTCAG CTCTGGGCAG CTTGGGCTCT GGAGGTTCTT CTGGAAGAGG TCGTGAACAA 
GGGATGGGCT CGGAGGAAGG AGAACAGGTT CCATACCCAG CCCTGGCACC CACCGTCTTC 
TTCTGTCTCA GCCAAACCAC GCGTCCACGG AACTGGTGCC TCAGACTGGT CTGTAATCCA 
TGGTTCGAGC ATGTCAGCAT GCTGGTCATT TTGCTGAACT GCGTCACCCT GGGGATGTTC 
CAGCCCTGCG AGGATACAAA CTGTATGTCA GAGAGATGCA ACATTCTAGA GGCATTCGAC 
GGCTTCATCT TTGCATTCTT TGCTGTGGAG ATGGTTATTA AGATGGTAGC GCTTGGCATC 
TTTGGGCAGA AGTGCTACCT GGGAGACACA TGGAACTGGC TGGATTTCTT CATTGTTATG 
GCAGGGATGT TGGAATATTC CCTCGATGGA CACAATGTCA GCCTATCCGC TATCCGCACT 
GTACGTGTTT TAAGGCCGCT AAGAGCCATT AACAGGGTTC CCAGTATGAG GATACTGGTG 
ACCCTGCTGC TAGATACTCT GCCTATGTTA GGCAACGTCC TTCTCTTGTG TTTCTTTGTG 
TTTTTCATCT TTGGAATCGT CGGCGTTCAG CTTTGGGCTG GGCTACTGCG CAACAGGTGT 
TTTATGGATA CAAATTTTGT AATGAGTTAC AATTTCACCC CTCTGAGGCC GTATTACCAG 
GTGGATGATG CAGAGGACAA CCCTTATATC TGCTCCTCTA ACCGAGACAA TGGGATGTTA 
AAGTGCCAGG ACATCCCTCC CAAAATGGAC TGCTCCCATG TATACTACTC AGATTTCCCC 
AGTAGGAATA GCTGCGTGAA TATTAATCAA TACTACAACG TATGCAAGCC GGGAGAGTTC 
AATCCACATA AAGGGGCCAT TAATTTTGAT AACATTGGCT ACGCCTGGAT AGCCATTTTT 
CAGGTTATAA CCCTAGAAGG ATGGGTTGAT ATCATGTACT ATGTTATGGA CGCACATTCT 
TTTTACAATT TTATATATTT TATACTACTT ATAATAGTGG GTTCTTTCTT CATGATAAAC 
TTGTGCCTGG TTGTGATAGC AACACAGTTT TCTGAAACAA AGCAGCGAGA GAACCAGCTA 
ATGCAGGAGC AACGGGCTCG TTACCTCTCC AACGACAGCA CAATCGCCAG CTTCTCGGAG 
CCAGGGAGCT GCTATGAGGA GCTGCTCAAA TACATCTGCC ACATCTTCAG GAAGGTTAAG 
CGAAGGACGT TAAGACTTTA TAATAACTGG CAGAATAAGA GGAGGAAAAA AGTTCAGCCC 
AACAGCAACA CGAACAGTCA GGGGCGTCAC CGCAAGTGCA AGCGGCGTAT CACCTCCATT 
CATCACTTAA TACACCACCA CCATCATCAC CACCACCATC ATTACCACAT CAGCAATGGG 
AGTCCCCGGG GTCCCAGGTC TAGTCCGGAG ATCTGTGATG TGGAGATGAA AATCATGAAG 
CCAAAGAATC AACTAAGGCT TCCTCCAGCA TCCCCCAGCC TCCAAAGCAT GTCCCCCAAC 
AGTGAGGCCG TCCACAGTAT CTACCACGCG GACTGCCACT TAGAGGCGCC TCAGCGGAAA 
TCGAGCAAGT CTTCCAATGC CTCGTCAAAC ATAAAACTGA ATGCTGGGTT GAATCACAGT 
AACATGAACT ATCCCACCAT CATGCCCTCC CAAGCCAACA AAACCAGCCC CGCAACACTA 
CCCAAAGGAA AAAGGAATGG CAGCAATGCA GTCAGCTTGC TAAGCAGCCC AGTCAGTATG 
AAGGCAGATT CGTTTGGGAA GACCAACCAC CTAGTCGGAG AGCATGGCCT GTGCAGGAGT 
TCCAGTAAGC TCTCCGGTTT GAGCGTGCCA TGCTCCTTGC CAAGCCCTCA GCCAAGCCCT 
CTACTGTGCG AAATCCAGAA CTGCCCGTAC TGTGCCAACC TTTTGGAGAA CCCTGACTTG 
GAGCTCAGCG ACTCTGAGAG TTGCGAATCG GATTCTAACG GGGTCTATGA ATTCACCCAG 
GACTTGAGGC GTGGAGACCA AAGAGACCAG GTGCAGAAAG GCAGAAAGCT CAAGACTAAG 
CCAAAGGAGG CTAACAAGGT GTATCTGCTC TGGGAAGAGT TTACCGTCCG ACTGAAAAGG 
ATTGTGGACA GCAAATATTT TAACCGCGGG ATTATGATTG CCATCCTGAT CAACACGCTG 
AGCATGGGGA TTGAGTATCA TGAGCAGCCA GAAGAACTGA CTAACGCCTT GGAAATCAGC 
AATATTGTGT TTACAAGCAT GTTTGCGCTG GAGATGCTGC TGAAACTCTT GGCCTTTGGG 
ATCTTTCAAT ACATCAAGAA TCCATACAAC ATCTTTGATG GGATCATTGT CGTCATCAGT 
GTGTGGGAAA TCATCGGTCA GTCGGACGGA GGTCTGTCTG TGCTTAGAAC ATTTCGCCTC 
CTTCGTGTGC TGAAGCTGGT ACGATTTATG CCAGCACTGC GGCGTCAGCT GGTGGTCCTG 
GTGAAGACGA TGGACAACGT TGCCACCTTT TGCATGCTTC TTATGCTCTT CATCTTTATA 
TTCAGCATCC TTGGCATGCA TCTGTTTGGG TGTAAGTTTA CTTGGACTGC AGACACCGGA 
GAGACAGTCA AAGACAGAAA GAACTTTGAT TCTTTGCTTT GGGCGATTGT CACCGTCTTC 
CAGATTCTCA CCCAAGAAGA CTGGAACATG GTCCTATACA ATGGCATGGC TTCCACCTCC 
TCCTGGGCAG CTCTCTACTT TGTGGCCCTG ATGACATTTG GAAATTATGT GCTTTTCAAC 
TTGTTAGTAG CCATCTTAGT CGAAGGCTTC CAAGCAGAGG GAGATGCAAC AAGGTCAGAT 
ACAGAGGAGG ACAAGGCTTC AGGCAACTTT GATGAAGATC TGGAGAAACT GAAGGAGCTT 
CGTGCTGCAG AACTGAAGAT GTACTCACTC GCAGTGACAC CCAACGGACA CCTGGACCCT 
CGGAGTTCCA TGCCCCCACC TATCATCATG CGCACCGCCG CCACCCCAAT GCCTACACCA 
AAGAGCTCCC CTTGCATGGA CTCATCACAT CCGTTCGGCG ATTCAAGAAG GGAAAGTAGC 
GTCTCGATAG ATCCATCAAT CGTTGATCAG AAGTCTTTGG CAAGTCTACG CAGCACCCCT 
TGTACCCCCT GGGGCACTTA CAGCAACTGG GGAAGCCGTC GCTCTAGCTG GAATAGTCTG 
GGTAGAGCCC CAAGCATCAA AAAGAAGAGC CAATCAGGGG AGAGGGAGTC CTTACTCTCT 
GGTGAAGGAA AGGGGAGCAC AGATGATGAC TCAGATGACT GCAAGTCAAG CCCAGCGGCG 
AGGGTATCTC TCCATCGCAG GGCAGGTTCC CTGGACACGC GGGGCTCTTT GGATATCCCA 
GATTTCCTGC AGGTGCCCAC GTTACGCCAT TCCCTAAGTG TCAGTCCTTT GGCCATGGTT 
CCTTCAGATT ATCAAGATTG CAATGGGAAA ATGATGCACG TGCCCAGTGA TCTGTTCGTT 
CGGGTGGAGA AACACAGGGA GGATCCGTTG GAGTATGAAG ACGACATAGA AGATAGTTAC 
TGTTTCCGAA TCCGAAAGAT GTTGGAGCCA TACAAACCTA CATGGTGTAA GACGCATGAA 
GACTGGTCCC TCTACATGTT CTCACCTCAA AACCAGTTCC GGATGATGTG CCAAAAAGTC 
ATTGCCCACA AGATGTTTGA CCACGTGGTT CTTGTCTTCA TCTTCTTAAA CTGCATCACC 
ATTGCACTTG AACGGCCTGA TATTGACCCC AATAGCCTGG AACGGATATT CTTGAGTGTC 
TCCAATTACA TTTTTACTGC AATCTTTGTT TTGGAAATGG CAATTAAGGT GGTGGCCCTG 
GGCTTTGTCT CCGGGGAGAA CACTTACCTG CAGAGCAGCT GGAACGTGCT TGATGGGATT 
CTGGTCTTCG TTTCCATCAT CGATATCGTC GTGTCCCTTG CCTCTGCCGG GGGGGCAAAG 
ATCCTGGGAG TCTTGCGGGT ACTGAGGTTG CTGCGGACCC TGCGCCCACT TAGGGTTATC 
AGCCGAGCCC CGGGCCTGAA GTTGGTGGTA GAAACTCTCA TTTCCTCCCT GAGGCCTATC 
GGGAACATTG TCCTTATCTG CTGCGCTTTC TTCATCATCT TTGGCATTCT GGGAGTACAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCGAA GGGTTAGAGA CGAGGAACAT CACCAACAGG 
TCAGAGTGCA CCCGGGCACG GTACAAATGG GTGCGGCGCA AATACAACTT TGACAACCTT 
GGCCAGGCGC TGATGTCGCT CTTTGTCCTC TCCTCCAAAG ATGGCTGGGT GAATATCATG 
TACGATGGCC TGGATGCCGT TGCCACAGAT GTTCAGCCAG TCAAAAACCA CAACCCATGG 
ATGCTCCTGT ACTTCATCTC CTTCCTCCTC ATCGTCAGCT TCTTCGTGCT CAACATGTTC 
GTAGGGGTGG TGGTAGAGAA CTTCCACAAG TGCCGGCAAC ACCAGGAGGC GGAAGAGGCC 
CGGCGCCGGG AGGAGAAACG CCTCCGCCGC CTGGAGAAAA AGCGCAGGAG TAAGGCGTTG 
TATATGTCTG AGGCCCAGCG GAGACCGTAT TACGCAGAGT ACTCGCCGGC GCGCCTCTAC 
ATCCACACCA TGTGTACCAG CCACTACCTG GATCTCTTTA TCACCTGCAT TATTTGTATC 
AACGTCATCA CCATGTCCAT GGAACACTAC AACCAACCCA AGTCCCTGGA TGAAGCTTTA 
AAATACTGCA ATTACGTGTT CACCATCGTC TTCGTGTTCG AGGCCCTGTT CAAGCTTGTG 
GCATTTGGGT TCCGGAGGTT TTTTAAAGAC AGGTGGAACC AACTGGACTT GGCCATTGTT 
TTGCTCTCCA TCATGGGAAT CACGTTAGAG GAGATTGAGA TTAACGCCTC CCTTCCCATC 
AATCCAACTA TTATACGCAT CATGAGGGTG CTGAGGATTG CTCGAGTGTT AAAGCTCCTG 
AAAATGGCCA CCGGGATGCG AGCTTTGCTG GATACTGTTG TGCAAGCGTT ACCTCAGGTA 
GGCAACCTTG GCCTGCTCTT CATGCTCCTG TTCTTCATCT ACGCAGCCCT GGGAGTGGAG 
CTGTTCGGAA AGCTCGTGTG CAATGAGGAG AATCCCTGTG AGGGACTCAG CCGACATGCA 
ACATTTGAGA ACTTTGGGAT GGCTTTTCTG ACCCTCTTCC GTGTTTCCAC AGGGGATAAC 
TGGAATGGCA TCATGAAGGA CACTCTACGA GAGTGCAACA GCGATGACAA GAACTGTCTC 
ACCTACCTGC CCGTCATCTC TCCCGTCTAT TTCGTAACCT TCGTGCTCAT TGCCCAGTTT 
GTCTTGGTCA ATGTGGTCGT GGCTGTGCTC ATGAAACATC TAGAGGAGAG CAATAAAGAG 
GCCAAAGAAG ATGCCGAGAT GGATGCAGAG ATTGAGATGG AGATGTCTAG GGCACTGACA 
CCTATAAGTG GTGGCTGCAC TCCAGCTTCA GATGGCAAGG GAACTGAGGG AGGGCAAGAT 
CCATTAAAGG GGGAGCTGCT GAAACAAGTG GGGGATGGGG ATTTCCAAAG GAGGGATGGA 
TCGGGCTCCC GAAACCTACT GGCTGTCAAA AAGATCTCGG TGTCTAGGAT GCTCTCCTTA 
CCTAATGACA GCTACATGTT TCGCCCAGTG CTACCAGCAA AGTCATCTTA TCCTTTGCAA 
GAAGTGGAGA CCACTGAAGA TAATTCACCA CATGGGTCGA CTGCTTCACT CCACTCCCAG 
CCAGGGGACA ATAACTCATT GCTGAATATA CCAGTGGAGT GCCAGCCACA GGCCTCTGTT 
AGCAACCACC AGGACTCTGC CGACCTGCCT AAAATCCCAC CGCCACATAC ATCCTCAAGA 
TCTCCAAGTC TAAACAGATC CCTGAGAAGA CAGGAGGCTA TACGTATGGA CTCAGTGGAT 
CAGGGTGGTG ATTCTAAGGA CCTTAATGTG GAATCTACAG AGAGTTTGCA TCTGGGAGCT 
CCATCAGTTA CTACTTTAGT TCCAAATTTG CTTTGCACCT TAAATGTGTC TCTGCAGGGA 
ACCCCCCAGT CCTCACCTAG GCTATCTCCA CAGCCTAGAC ATTCCAGTGT CCTCACACAC 
AACCATGTAT ACAGCCAACA CAGTATTCCA AGTCGGCCTT CGAGTGTCAG TAGTGGCAAC 
AGCAACCCAG AGGAATCTAT GTTTGAGTCC TCAGACCTGG CTGATGAAGA AGTCAGCCAT 
ATAAACAGTT CAGCAAAAGA CTGGAGAGGA TCATCTAATA ATAGAAGTCA CTCTGTTTCT 
CCATTCATCT CCCAATCTTC ATCTCCTCCT CCTCTTTCTC CGTCACTAAA GAACTGCAAT 
AGCACAGTCA GCCTGGCAGG GTCCAGCGGC AAAGATAAAG ACTTGAAGAA GTTCTATAGT 
GTTGATACTC AAGGATTCTT AGCTAAGCCC TGCTGGGGGG ATGACCAAAG GAGGCACTCA 
ATTGAGATCT GCCCTTCCAT TGATGATGGA GATTCCTGCT TTGACCTGTC CATGGAGGAC 
AAACATTGCT CTTCTGTTCT CATTCGGTCT GACCCCGAAT ACATCAATGG AGTCCGACGA 
AAGAAGAAGA TGAGTCCCCC TTGCATTTCT ATAGACCCTC CTATGGAGGA TGAAGGTGGC 
ACAGCTTCCC GTACCAAAGC ATCAGAAAAC AGCATGATGT TACGAAGAAG GACCCCATCC 
TGTGAATCTA CTGCATACAG GGACTCTTTA GATCTTAGTG ACAACCAGCT TGGGGACCAA 
ATATCTAAAA CTGATAGACG AACCCAGGTG CCCTGTCGCA ATGAACACTT GATCATACCC 
AGCTACTCGT TTGACAAGGT GGACGGAAAC GGCTTCTCAG ACCTAATATC TAACAGTGGG 
GGTTCTACTC CGACGTCCGT TTTCAATGAA CCTGTGTCTT TAGAAATGAA ACAAAAAGAT 
CACAACGATC TGAGCGGCGT TTCTAAAGTT TCGTTGAAAA CTGCACTAGA TCACGTATCC 
AACCCAACAG AGGACATAAT CGATAACCCA TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748785.1
CDS	448..7683
Translation	MTDGERKEPFQPLAGDDIRVPIPPTPQAGDPSEEEDAGNAEKSALGSLGSGGSSGRGREQGMGSEEGEQVPYPALAPTVFFCLSQTTRPRNWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKCKRRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSSPEICDVEMKIMKPKNQLRLPPASPSLQSMSPNSEAVHSIYHADCHLEAPQRKSSKSSNASSNIKLNAGLNHSNMNYPTIMPSQANKTSPATLPKGKRNGSNAVSLLSSPVSMKADSFGKTNHLVGEHGLCRSSSKLSGLSVPCSLPSPQPSPLLCEIQNCPYCANLLENPDLELSDSESCESDSNGVYEFTQDLRRGDQRDQVQKGRKLKTKPKEANKVYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTEEDKASGNFDEDLEKLKELRAAELKMYSLAVTPNGHLDPRSSMPPPIIMRTAATPMPTPKSSPCMDSSHPFGDSRRESSVSIDPSIVDQKSLASLRSTPCTPWGTYSNWGSRRSSWNSLGRAPSIKKKSQSGERESLLSGEGKGSTDDDSDDCKSSPAARVSLHRRAGSLDTRGSLDIPDFLQVPTLRHSLSVSPLAMVPSDYQDCNGKMMHVPSDLFVRVEKHREDPLEYEDDIEDSYCFRIRKMLEPYKPTWCKTHEDWSLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKALYMSEAQRRPYYAEYSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNSDDKNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEMSRALTPISGGCTPASDGKGTEGGQDPLKGELLKQVGDGDFQRRDGSGSRNLLAVKKISVSRMLSLPNDSYMFRPVLPAKSSYPLQEVETTEDNSPHGSTASLHSQPGDNNSLLNIPVECQPQASVSNHQDSADLPKIPPPHTSSRSPSLNRSLRRQEAIRMDSVDQGGDSKDLNVESTESLHLGAPSVTTLVPNLLCTLNVSLQGTPQSSPRLSPQPRHSSVLTHNHVYSQHSIPSRPSSVSSGNSNPEESMFESSDLADEEVSHINSSAKDWRGSSNNRSHSVSPFISQSSSPPPLSPSLKNCNSTVSLAGSSGKDKDLKKFYSVDTQGFLAKPCWGDDQRRHSIEICPSIDDGDSCFDLSMEDKHCSSVLIRSDPEYINGVRRKKKMSPPCISIDPPMEDEGGTASRTKASENSMMLRRRTPSCESTAYRDSLDLSDNQLGDQISKTDRRTQVPCRNEHLIIPSYSFDKVDGNGFSDLISNSGGSTPTSVFNEPVSLEMKQKDHNDLSGVSKVSLKTALDHVSNPTEDIIDNPL

Target ORF information:

RefSeq Version	XM_031892925.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892925.1

ORF Insert Sequence:

ATGACTGATG GCGAGAGGAA AGAGCCCTTC CAGCCACTGG CCGGAGACGA TATCCGGGTG 
CCCATACCCC CGACACCCCA AGCAGGGGAC CCCAGTGAAG AAGAGGATGC GGGAAATGCA 
GAGAAGTCAG CTCTGGGCAG CTTGGGCTCT GGAGGTTCTT CTGGAAGAGG TCGTGAACAA 
GGGATGGGCT CGGAGGAAGG AGAACAGGTT CCATACCCAG CCCTGGCACC CACCGTCTTC 
TTCTGTCTCA GCCAAACCAC GCGTCCACGG AACTGGTGCC TCAGACTGGT CTGTAATCCA 
TGGTTCGAGC ATGTCAGCAT GCTGGTCATT TTGCTGAACT GCGTCACCCT GGGGATGTTC 
CAGCCCTGCG AGGATACAAA CTGTATGTCA GAGAGATGCA ACATTCTAGA GGCATTCGAC 
GGCTTCATCT TTGCATTCTT TGCTGTGGAG ATGGTTATTA AGATGGTAGC GCTTGGCATC 
TTTGGGCAGA AGTGCTACCT GGGAGACACA TGGAACTGGC TGGATTTCTT CATTGTTATG 
GCAGGGATGT TGGAATATTC CCTCGATGGA CACAATGTCA GCCTATCCGC TATCCGCACT 
GTACGTGTTT TAAGGCCGCT AAGAGCCATT AACAGGGTTC CCAGTATGAG GATACTGGTG 
ACCCTGCTGC TAGATACTCT GCCTATGTTA GGCAACGTCC TTCTCTTGTG TTTCTTTGTG 
TTTTTCATCT TTGGAATCGT CGGCGTTCAG CTTTGGGCTG GGCTACTGCG CAACAGGTGT 
TTTATGGATA CAAATTTTGT AATGAGTTAC AATTTCACCC CTCTGAGGCC GTATTACCAG 
GTGGATGATG CAGAGGACAA CCCTTATATC TGCTCCTCTA ACCGAGACAA TGGGATGTTA 
AAGTGCCAGG ACATCCCTCC CAAAATGGAC TGCTCCCATG TATACTACTC AGATTTCCCC 
AGTAGGAATA GCTGCGTGAA TATTAATCAA TACTACAACG TATGCAAGCC GGGAGAGTTC 
AATCCACATA AAGGGGCCAT TAATTTTGAT AACATTGGCT ACGCCTGGAT AGCCATTTTT 
CAGGTTATAA CCCTAGAAGG ATGGGTTGAT ATCATGTACT ATGTTATGGA CGCACATTCT 
TTTTACAATT TTATATATTT TATACTACTT ATAATAGTGG GTTCTTTCTT CATGATAAAC 
TTGTGCCTGG TTGTGATAGC AACACAGTTT TCTGAAACAA AGCAGCGAGA GAACCAGCTA 
ATGCAGGAGC AACGGGCTCG TTACCTCTCC AACGACAGCA CAATCGCCAG CTTCTCGGAG 
CCAGGGAGCT GCTATGAGGA GCTGCTCAAA TACATCTGCC ACATCTTCAG GAAGGTTAAG 
CGAAGGACGT TAAGACTTTA TAATAACTGG CAGAATAAGA GGAGGAAAAA AGTTCAGCCC 
AACAGCAACA CGAACAGTCA GGGGCGTCAC CGCAAGTGCA AGCGGCGTAT CACCTCCATT 
CATCACTTAA TACACCACCA CCATCATCAC CACCACCATC ATTACCACAT CAGCAATGGG 
AGTCCCCGGG GTCCCAGGTC TAGTCCGGAG ATCTGTGATG TGGAGATGAA AATCATGAAG 
CCAAAGAATC AACTAAGGCT TCCTCCAGCA TCCCCCAGCC TCCAAAGCAT GTCCCCCAAC 
AGTGAGGCCG TCCACAGTAT CTACCACGCG GACTGCCACT TAGAGGCGCC TCAGCGGAAA 
TCGAGCAAGT CTTCCAATGC CTCGTCAAAC ATAAAACTGA ATGCTGGGTT GAATCACAGT 
AACATGAACT ATCCCACCAT CATGCCCTCC CAAGCCAACA AAACCAGCCC CGCAACACTA 
CCCAAAGGAA AAAGGAATGG CAGCAATGCA GTCAGCTTGC TAAGCAGCCC AGTCAGTATG 
AAGGCAGATT CGTTTGGGAA GACCAACCAC CTAGTCGGAG AGCATGGCCT GTGCAGGAGT 
TCCAGTAAGC TCTCCGGTTT GAGCGTGCCA TGCTCCTTGC CAAGCCCTCA GCCAAGCCCT 
CTACTGTGCG AAATCCAGAA CTGCCCGTAC TGTGCCAACC TTTTGGAGAA CCCTGACTTG 
GAGCTCAGCG ACTCTGAGAG TTGCGAATCG GATTCTAACG GGGTCTATGA ATTCACCCAG 
GACTTGAGGC GTGGAGACCA AAGAGACCAG GTGCAGAAAG GCAGAAAGCT CAAGACTAAG 
CCAAAGGAGG CTAACAAGGT GTATCTGCTC TGGGAAGAGT TTACCGTCCG ACTGAAAAGG 
ATTGTGGACA GCAAATATTT TAACCGCGGG ATTATGATTG CCATCCTGAT CAACACGCTG 
AGCATGGGGA TTGAGTATCA TGAGCAGCCA GAAGAACTGA CTAACGCCTT GGAAATCAGC 
AATATTGTGT TTACAAGCAT GTTTGCGCTG GAGATGCTGC TGAAACTCTT GGCCTTTGGG 
ATCTTTCAAT ACATCAAGAA TCCATACAAC ATCTTTGATG GGATCATTGT CGTCATCAGT 
GTGTGGGAAA TCATCGGTCA GTCGGACGGA GGTCTGTCTG TGCTTAGAAC ATTTCGCCTC 
CTTCGTGTGC TGAAGCTGGT ACGATTTATG CCAGCACTGC GGCGTCAGCT GGTGGTCCTG 
GTGAAGACGA TGGACAACGT TGCCACCTTT TGCATGCTTC TTATGCTCTT CATCTTTATA 
TTCAGCATCC TTGGCATGCA TCTGTTTGGG TGTAAGTTTA CTTGGACTGC AGACACCGGA 
GAGACAGTCA AAGACAGAAA GAACTTTGAT TCTTTGCTTT GGGCGATTGT CACCGTCTTC 
CAGATTCTCA CCCAAGAAGA CTGGAACATG GTCCTATACA ATGGCATGGC TTCCACCTCC 
TCCTGGGCAG CTCTCTACTT TGTGGCCCTG ATGACATTTG GAAATTATGT GCTTTTCAAC 
TTGTTAGTAG CCATCTTAGT CGAAGGCTTC CAAGCAGAGG GAGATGCAAC AAGGTCAGAT 
ACAGAGGAGG ACAAGGCTTC AGGCAACTTT GATGAAGATC TGGAGAAACT GAAGGAGCTT 
CGTGCTGCAG AACTGAAGAT GTACTCACTC GCAGTGACAC CCAACGGACA CCTGGACCCT 
CGGAGTTCCA TGCCCCCACC TATCATCATG CGCACCGCCG CCACCCCAAT GCCTACACCA 
AAGAGCTCCC CTTGCATGGA CTCATCACAT CCGTTCGGCG ATTCAAGAAG GGAAAGTAGC 
GTCTCGATAG ATCCATCAAT CGTTGATCAG AAGTCTTTGG CAAGTCTACG CAGCACCCCT 
TGTACCCCCT GGGGCACTTA CAGCAACTGG GGAAGCCGTC GCTCTAGCTG GAATAGTCTG 
GGTAGAGCCC CAAGCATCAA AAAGAAGAGC CAATCAGGGG AGAGGGAGTC CTTACTCTCT 
GGTGAAGGAA AGGGGAGCAC AGATGATGAC TCAGATGACT GCAAGTCAAG CCCAGCGGCG 
AGGGTATCTC TCCATCGCAG GGCAGGTTCC CTGGACACGC GGGGCTCTTT GGATATCCCA 
GATTTCCTGC AGGTGCCCAC GTTACGCCAT TCCCTAAGTG TCAGTCCTTT GGCCATGGTT 
CCTTCAGATT ATCAAGATTG CAATGGGAAA ATGATGCACG TGCCCAGTGA TCTGTTCGTT 
CGGGTGGAGA AACACAGGGA GGATCCGTTG GAGTATGAAG ACGACATAGA AGATAGTTAC 
TGTTTCCGAA TCCGAAAGAT GTTGGAGCCA TACAAACCTA CATGGTGTAA GACGCATGAA 
GACTGGTCCC TCTACATGTT CTCACCTCAA AACCAGTTCC GGATGATGTG CCAAAAAGTC 
ATTGCCCACA AGATGTTTGA CCACGTGGTT CTTGTCTTCA TCTTCTTAAA CTGCATCACC 
ATTGCACTTG AACGGCCTGA TATTGACCCC AATAGCCTGG AACGGATATT CTTGAGTGTC 
TCCAATTACA TTTTTACTGC AATCTTTGTT TTGGAAATGG CAATTAAGGT GGTGGCCCTG 
GGCTTTGTCT CCGGGGAGAA CACTTACCTG CAGAGCAGCT GGAACGTGCT TGATGGGATT 
CTGGTCTTCG TTTCCATCAT CGATATCGTC GTGTCCCTTG CCTCTGCCGG GGGGGCAAAG 
ATCCTGGGAG TCTTGCGGGT ACTGAGGTTG CTGCGGACCC TGCGCCCACT TAGGGTTATC 
AGCCGAGCCC CGGGCCTGAA GTTGGTGGTA GAAACTCTCA TTTCCTCCCT GAGGCCTATC 
GGGAACATTG TCCTTATCTG CTGCGCTTTC TTCATCATCT TTGGCATTCT GGGAGTACAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCGAA GGGTTAGAGA CGAGGAACAT CACCAACAGG 
TCAGAGTGCA CCCGGGCACG GTACAAATGG GTGCGGCGCA AATACAACTT TGACAACCTT 
GGCCAGGCGC TGATGTCGCT CTTTGTCCTC TCCTCCAAAG ATGGCTGGGT GAATATCATG 
TACGATGGCC TGGATGCCGT TGCCACAGAT GTTCAGCCAG TCAAAAACCA CAACCCATGG 
ATGCTCCTGT ACTTCATCTC CTTCCTCCTC ATCGTCAGCT TCTTCGTGCT CAACATGTTC 
GTAGGGGTGG TGGTAGAGAA CTTCCACAAG TGCCGGCAAC ACCAGGAGGC GGAAGAGGCC 
CGGCGCCGGG AGGAGAAACG CCTCCGCCGC CTGGAGAAAA AGCGCAGGAG TAAGGCGTTG 
TATATGTCTG AGGCCCAGCG GAGACCGTAT TACGCAGAGT ACTCGCCGGC GCGCCTCTAC 
ATCCACACCA TGTGTACCAG CCACTACCTG GATCTCTTTA TCACCTGCAT TATTTGTATC 
AACGTCATCA CCATGTCCAT GGAACACTAC AACCAACCCA AGTCCCTGGA TGAAGCTTTA 
AAATACTGCA ATTACGTGTT CACCATCGTC TTCGTGTTCG AGGCCCTGTT CAAGCTTGTG 
GCATTTGGGT TCCGGAGGTT TTTTAAAGAC AGGTGGAACC AACTGGACTT GGCCATTGTT 
TTGCTCTCCA TCATGGGAAT CACGTTAGAG GAGATTGAGA TTAACGCCTC CCTTCCCATC 
AATCCAACTA TTATACGCAT CATGAGGGTG CTGAGGATTG CTCGAGTGTT AAAGCTCCTG 
AAAATGGCCA CCGGGATGCG AGCTTTGCTG GATACTGTTG TGCAAGCGTT ACCTCAGGTA 
GGCAACCTTG GCCTGCTCTT CATGCTCCTG TTCTTCATCT ACGCAGCCCT GGGAGTGGAG 
CTGTTCGGAA AGCTCGTGTG CAATGAGGAG AATCCCTGTG AGGGACTCAG CCGACATGCA 
ACATTTGAGA ACTTTGGGAT GGCTTTTCTG ACCCTCTTCC GTGTTTCCAC AGGGGATAAC 
TGGAATGGCA TCATGAAGGA CACTCTACGA GAGTGCAACA GCGATGACAA GAACTGTCTC 
ACCTACCTGC CCGTCATCTC TCCCGTCTAT TTCGTAACCT TCGTGCTCAT TGCCCAGTTT 
GTCTTGGTCA ATGTGGTCGT GGCTGTGCTC ATGAAACATC TAGAGGAGAG CAATAAAGAG 
GCCAAAGAAG ATGCCGAGAT GGATGCAGAG ATTGAGATGG AGATGTCTAG GGCACTGACA 
CCTATAAGTG GTGGCTGCAC TCCAGCTTCA GATGGCAAGG GAACTGAGGG AGGGCAAGAT 
CCATTAAAGG GGGAGCTGCT GAAACAAGTG GGGGATGGGG ATTTCCAAAG GAGGGATGGA 
TCGGGCTCCC GAAACCTACT GGCTGTCAAA AAGATCTCGG TGTCTAGGAT GCTCTCCTTA 
CCTAATGACA GCTACATGTT TCGCCCAGTG CTACCAGCAA AGTCATCTTA TCCTTTGCAA 
GAAGTGGAGA CCACTGAAGA TAATTCACCA CATGGGTCGA CTGCTTCACT CCACTCCCAG 
CCAGGGGACA ATAACTCATT GCTGAATATA CCAGTGGAGT GCCAGCCACA GGCCTCTGTT 
AGCAACCACC AGGACTCTGC CGACCTGCCT AAAATCCCAC CGCCACATAC ATCCTCAAGA 
TCTCCAAGTC TAAACAGATC CCTGAGAAGA CAGGAGGCTA TACGTATGGA CTCAGTGGAT 
CAGGGTGGTG ATTCTAAGGA CCTTAATGTG GAATCTACAG AGAGTTTGCA TCTGGGAGCT 
CCATCAGTTA CTACTTTAGT TCCAAATTTG CTTTGCACCT TAAATGTGTC TCTGCAGGGA 
ACCCCCCAGT CCTCACCTAG GCTATCTCCA CAGCCTAGAC ATTCCAGTGT CCTCACACAC 
AACCATGTAT ACAGCCAACA CAGTATTCCA AGTCGGCCTT CGAGTGTCAG TAGTGGCAAC 
AGCAACCCAG AGGAATCTAT GTTTGAGTCC TCAGACCTGG CTGATGAAGA AGTCAGCCAT 
ATAAACAGTT CAGCAAAAGA CTGGAGAGGA TCATCTAATA ATAGAAGTCA CTCTGTTTCT 
CCATTCATCT CCCAATCTTC ATCTCCTCCT CCTCTTTCTC CGTCACTAAA GAACTGCAAT 
AGCACAGTCA GCCTGGCAGG GTCCAGCGGC AAAGATAAAG ACTTGAAGAA GTTCTATAGT 
GTTGATACTC AAGGATTCTT AGCTAAGCCC TGCTGGGGGG ATGACCAAAG GAGGCACTCA 
ATTGAGATCT GCCCTTCCAT TGATGATGGA GATTCCTGCT TTGACCTGTC CATGGAGGAC 
AAACATTGCT CTTCTGTTCT CATTCGGTCT GACCCCGAAT ACATCAATGG AGTCCGACGA 
AAGAAGAAGA TGAGTCCCCC TTGCATTTCT ATAGACCCTC CTATGGAGGA TGAAGGTGGC 
ACAGCTTCCC GTACCAAAGC ATCAGAAAAC AGCATGATGT TACGAAGAAG GACCCCATCC 
TGTGAATCTA CTGCATACAG GGACTCTTTA GATCTTAGTG ACAACCAGCT TGGGGACCAA 
ATATCTAAAA CTGATAGACG AACCCAGGTG CCCTGTCGCA ATGAACACTT GATCATACCC 
AGCTACTCGT TTGACAAGGT GGACGGAAAC GGCTTCTCAG ACCTAATATC TAACAGTGGG 
GGTTCTACTC CGACGTCCGT TTTCAATGAA CCTGTGTCTT TAGAAATGAA ACAAAAAGAT 
CACAACGATC TGAGCGGCGT TTCTAAAGTT TCGTTGAAAA CTGCACTAGA TCACGTATCC 
AACCCAACAG AGGACATAAT CGATAACCCA TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1h ( XM_018097254.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952743.1 Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), mRNA.

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CloneID	OXa46699
Clone ID Related Accession (Same CDS sequence)	XM_018097254.1
Accession Version	XM_018097254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent T-type calcium channel subunit alpha-1H
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGTCCGCG TGTTCCGCCA GTCCGATCCA GTACTGGCAG GGTCCAATGC TGTGGAAATA 
TGGAACCGGC CAGACCAGTC CGATCCAGTA CTGGTAGGGT CCAATGCAGT GGAAATGTTG 
GACCCAGCCA GGCCAATCCG ATCCAGTACT GGAGATAAAC ATGGCGACTG CCAAGGGGAA 
AAAGATGGCA ACTGCCAAGG AGACAAAGAT GGCGACTGCC AAGGGGACAA AAATGGCGAC 
TGCCAAGGGG ACAAAGATGG CGACTGCCAA GGGTACAAAG ATGGCGACTG CCAAGGGGCA 
AAGATGGCTA CTGCCAAGGA GACAAAGATG GCGACTGCCA AGGGGACAAA GATGGCGACT 
GCCAAGGGGA CAAAGATGGC GACTGCCAAG GGGACAAAGA TGGCGACTGC CAAGGCATCA 
CTTACTCTAT GTTCCAACCA AACTCCAAGA ACTATTTGCC TTAACTTACC CTTTCCTACA 
TGGTTCGAGC ATGTCAGCAT GCTGGTCATT TTGCTGAACT GCGTCACCCT GGGGATGTTC 
CAGCCCTGCG AGGATACAAA CTGTATGTCA GAGAGATGCA ACATTCTAGA GGCATTCGAC 
GGCTTCATCT TTGCATTCTT TGCTGTGGAG ATGGTTATTA AGATGGTAGC GCTTGGCATC 
TTTGGGCAGA AGTGCTACCT GGGAGACACA TGGAACTGGC TGGATTTCTT CATTGTTATG 
GCAGGGATGT TGGAATATTC CCTCGATGGA CACAATGTCA GCCTATCCGC TATCCGCACT 
GTACGTGTTT TAAGGCCGCT AAGAGCCATT AACAGGGTTC CCAGTATGAG GATACTGGTG 
ACCCTGCTGC TAGATACTCT GCCTATGTTA GGCAACGTCC TTCTCTTGTG TTTCTTTGTG 
TTTTTCATCT TTGGAATCGT CGGCGTTCAG CTTTGGGCTG GGCTACTGCG CAACAGGTGT 
TTTATGGATA CAAATTTTGT AATGAGTTAC AATTTCACCC CTCTGAGGCC GTATTACCAG 
GTGGATGATG CAGAGGACAA CCCTTATATC TGCTCCTCTA ACCGAGACAA TGGGATGTTA 
AAGTGCCAGG ACATCCCTCC CAAAATGGAC TGCTCCCATG TATACTACTC AGATTTCCCC 
AGTAGGAATA GCTGCGTGAA TATTAATCAA TACTACAACG TATGCAAGCC GGGAGAGTTC 
AATCCACATA AAGGGGCCAT TAATTTTGAT AACATTGGCT ACGCCTGGAT AGCCATTTTT 
CAGGTTATAA CCCTAGAAGG ATGGGTTGAT ATCATGTACT ATGTTATGGA CGCACATTCT 
TTTTACAATT TTATATATTT TATACTACTT ATAATAGTGG GTTCTTTCTT CATGATAAAC 
TTGTGCCTGG TTGTGATAGC AACACAGTTT TCTGAAACAA AGCAGCGAGA GAACCAGCTA 
ATGCAGGAGC AACGGGCTCG TTACCTCTCC AACGACAGCA CAATCGCCAG CTTCTCGGAG 
CCAGGGAGCT GCTATGAGGA GCTGCTCAAA TACATCTGCC ACATCTTCAG GAAGGTTAAG 
CGAAGGACGT TAAGACTTTA TAATAACTGG CAGAATAAGA GGAGGAAAAA AGTTCAGCCC 
AACAGCAACA CGAACAGTCA GGGGCGTCAC CGCAAGTGCA AGCGGCGTAT CACCTCCATT 
CATCACTTAA TACACCACCA CCATCATCAC CACCACCATC ATTACCACAT CAGCAATGGG 
AGTCCCCGGG GTCCCAGGTC TAGTCCGGAG ATCTGTGATG TGGAGATGAA AATCATGAAG 
CCAAAGAATC AACTAAGGCT TCCTCCAGCA TCCCCCAGCC TCCAAAGCAT GTCCCCCAAC 
AGTGAGGCCG TCCACAGTAT CTACCACGCG GACTGCCACT TAGAGGCGCC TCAGCGGAAA 
TCGAGCAAGT CTTCCAATGC CTCGTCAAAC ATAAAACTGA ATGCTGGGTT GAATCACAGT 
AACATGAACT ATCCCACCAT CATGCCCTCC CAAGCCAACA AAACCAGCCC CGCAACACTA 
CCCAAAGGAA AAAGGAATGG CAGCAATGCA GTCAGCTTGC TAAGCAGCCC AGTCAGTATG 
AAGGCAGATT CGTTTGGGAA GACCAACCAC CTAGTCGGAG AGCATGGCCT GTGCAGGAGT 
TCCAGTAAGC TCTCCGGTTT GAGCGTGCCA TGCTCCTTGC CAAGCCCTCA GCCAAGCCCT 
CTACTGTGCG AAATCCAGAA CTGCCCGTAC TGTGCCAACC TTTTGGAGAA CCCTGACTTG 
GAGCTCAGCG ACTCTGAGAG TTGCGAATCG GATTCTAACG GGGTCTATGA ATTCACCCAG 
GACTTGAGGC GTGGAGACCA AAGAGACCAG GTGCAGAAAG GCAGAAAGCT CAAGACTAAG 
CCAAAGGAGG CTAACAAGGT GTATCTGCTC TGGGAAGAGT TTACCGTCCG ACTGAAAAGG 
ATTGTGGACA GCAAATATTT TAACCGCGGG ATTATGATTG CCATCCTGAT CAACACGCTG 
AGCATGGGGA TTGAGTATCA TGAGCAGCCA GAAGAACTGA CTAACGCCTT GGAAATCAGC 
AATATTGTGT TTACAAGCAT GTTTGCGCTG GAGATGCTGC TGAAACTCTT GGCCTTTGGG 
ATCTTTCAAT ACATCAAGAA TCCATACAAC ATCTTTGATG GGATCATTGT CGTCATCAGT 
GTGTGGGAAA TCATCGGTCA GTCGGACGGA GGTCTGTCTG TGCTTAGAAC ATTTCGCCTC 
CTTCGTGTGC TGAAGCTGGT ACGATTTATG CCAGCACTGC GGCGTCAGCT GGTGGTCCTG 
GTGAAGACGA TGGACAACGT TGCCACCTTT TGCATGCTTC TTATGCTCTT CATCTTTATA 
TTCAGCATCC TTGGCATGCA TCTGTTTGGG TGTAAGTTTA CTTGGACTGC AGACACCGGA 
GAGACAGTCA AAGACAGAAA GAACTTTGAT TCTTTGCTTT GGGCGATTGT CACCGTCTTC 
CAGATTCTCA CCCAAGAAGA CTGGAACATG GTCCTATACA ATGGCATGGC TTCCACCTCC 
TCCTGGGCAG CTCTCTACTT TGTGGCCCTG ATGACATTTG GAAATTACGT GCTTTTCAAC 
TTGTTAGTAG CCATCTTAGT CGAAGGCTTC CAAGCAGAGG GAGATGCAAC AAGGTCAGAT 
ACAGAGGAGG ACAAGGCTTC AGGCAACTTT GATGAAGATC TGGAGAAACT GAAGGAGCTT 
CGTGCTGCAG AACTGAAGAT GTACTCACTC GCAGTGACAC CCAACGGACA CCTGGACCCT 
CGGAGTTCCA TGCCCCCACC TATCATCATG CGCACCGCCG CCACCCCAAT GCCTACACCA 
AAGAGCTCCC CTTGCATGGA CTCATCACAT CCATTCGGCG ATTCAAGAAG GGAAAGTAGC 
GTCTCGATAG ATCCATCAAT CGTTGATCAG AAGTCTTTGG CAAGTCTACG CAGCACCCCT 
TGTACCCCCT GGGGCACTTA CAGCAACTGG GGAAGCCGTC GCTCTAGCTG GAATAGTCTG 
GGAAGAGCCC CAAGCATCAA AAAGAAGAGC CAATCAGGGG AGAGGGAGTC CTTACTCTCT 
GGTGAAGGAA AGGGGAGCAC AGATGATGAC TCAGATGACT GCAAGTCAAG CCCAGCGGCG 
AGGGTATCTC TCCATCGCAG GGCAGGTTCC CTGGACACGC GGGGCTCTTT GGATATCCCA 
GATTTCCTGC AGGTGCCCAC GTTACGCCAT TCCCTAAGTG TCAGTCCTTT GGCCATGGTT 
CCTTCAGATT ATCAAGATTG CAATGGGAAA ATGATGCACG TGCCCAGTGA TCTGTTCGTT 
CGGGTGGAGA AACACAGGGA GGATCCGTTG GAGTATGAAG ACGACATAGA AGATAGTTAC 
TGTTTCCGAA TCCGAAAGAT GTTGGAGCCA TACAAACCTA CATGGTGTAA GACGCATGAA 
GACTGGTCCC TCTACATGTT CTCACCTCAA AACCAGTTCC GGATGATGTG CCAAAAAGTC 
ATTGCCCACA AGATGTTTGA CCACGTGGTT CTTGTCTTCA TCTTCTTAAA CTGCATCACC 
ATTGCACTTG AACGGCCTGA TATTGACCCC AATAGCCTGG AACGGATATT CTTGAGTGTC 
TCCAATTACA TTTTTACTGC AATCTTTGTT TTGGAAATGG CAATTAAGGT GGTGGCCCTG 
GGCTTTGTCT CCGGGGAGAA CACTTACCTG CAGAGCAGCT GGAACGTGCT CGATGGGATT 
CTGGTCTTCG TTTCCATCAT CGATATCGTC GTGTCCCTTG CCTCTGCCGG GGGGGCAAAG 
ATCCTGGGAG TCTTGCGGGT ACTGAGGTTG CTGCGGACCC TGCGCCCACT TAGGGTTATC 
AGCCGAGCCC CGGGCCTGAA GTTGGTGGTA GAAACTCTCA TTTCCTCCCT GAGGCCTATC 
GGGAACATTG TCCTTATCTG CTGCGCTTTC TTCATCATCT TTGGCATTCT GGGAGTACAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCGAA GGGTTAGAGA CGAGGAACAT CACCAACAGG 
TCAGAGTGCA CCCGGGCACG GTACAAATGG GTGCGGCGCA AATACAACTT TGACAACCTT 
GGCCAGGCGC TGATGTCGCT CTTTGTCCTC TCCTCCAAAG ATGGCTGGGT GAATATCATG 
TACGATGGCC TGGATGCCGT TGCCACAGAT GTTCAGCCAG TCAAAAACCA CAACCCATGG 
ATGCTCCTGT ACTTCATCTC CTTCCTCCTC ATCGTCAGCT TCTTCGTGCT CAACATGTTC 
GTAGGGGTGG TGGTAGAGAA CTTCCACAAG TGCCGGCAAC ACCAGGAGGC GGAAGAGGCC 
CGGCGCCGGG AGGAGAAACG CCTCCGCCGC CTGGAGAAAA AGCGCAGGAA GGCCCAGCGG 
AGACCGTATT ACGCAGAGTA CTCGCCGGCG CGCCTCTACA TCCACACCAT GTGTACCAGC 
CACTACCTGG ATCTCTTTAT CACCTGCATT ATTTGTATCA ACGTCATCAC CATGTCCATG 
GAACACTACA ACCAACCCAA GTCCCTGGAT GAAGCTTTAA AATACTGCAA TTACGTGTTC 
ACCATCGTCT TCGTGTTCGA GGCCCTGTTC AAGCTTGTGG CATTTGGGTT CCGGAGGTTT 
TTTAAAGACA GGTGGAACCA ACTGGACTTG GCCATCGTTT TGCTCTCCAT CATGGGAATC 
ACGTTAGAGG AGATTGAGAT TAACGCCTCA CTTCCCATCA ATCCAACTAT TATACGCATC 
ATGAGGGTGC TGAGGATTGC TCGAGTGTTA AAGCTCCTGA AAATGGCCAC TGGGATGCGA 
GCTTTGCTGG ATACTGTTGT GCAAGCGTTA CCTCAGGTAG GCAACCTTGG CCTGCTCTTC 
ATGCTCCTGT TCTTCATCTA CGCAGCCCTG GGAGTGGAGC TGTTCGGAAA GCTCGTGTGC 
AATGAGGAGA ATCCCTGTGA GGGACTCAGC CGACATGCAA CATTTGAGAA CTTTGGGATG 
GCTTTTCTGA CCCTCTTCCG TGTTTCCACA GGGGATAACT GGAATGGCAT CATGAAGGAC 
ACTCTACGAG AGTGCAACAG CGATGACAAG AACTGTCTCA CCTACCTGCC CGTCATCTCT 
CCCGTCTATT TCGTAACCTT CGTGCTCATT GCCCAGTTTG TCTTGGTCAA TGTGGTCGTG 
GCTGTGCTCA TGAAACATCT AGAGGAGAGC AATAAAGAGG CCAAAGAAGA TGCCGAGATG 
GATGCAGAGA TTGAGATGGA GATGTCTAGG GCACTGACAC CTATAAGTGG TGGCTGCACT 
CCAGCTTCAG ATGGCAAGGG AACTGAGGGA GGGCAAGATC CATTAAAGGG GGAGCTGCTG 
AAACAAGTGG GGGGGGCAGT GTGCCAAGAG AATGTGACCT TAGTGATCCC AGAGTCAATG 
GAGGGTGCTG CCCTGCTGAA CGACGCAGTC CCTGCTTGCT CCATATACCA ACAGCAGGCC 
CTGCCACTTC CCCTCCAGCC TTGCGTCCTT GGGCACGAGG ATGGGGATTT CCAAAGGAGG 
GATGGATCGG GCTCCCGAAA CCTACTGGCT GTCAAAAAGA TCTCGGTGTC TAGGATGCTC 
TCCTTACCTA ATGACAGCTA CATGTTTCGC CCAGTGCTAC CAGCAAAGTC ATCTTATCCT 
TTGCAAGAAG TGGAGACCAC TGAAGATAAT TCACCACATG GGTCGACTGC TTCACTCCAC 
TCCCAGCCAG GGGACAATAA CTCATTGCTG AATATACCAG TGGAGTGCCA GCCACAGGCC 
TCTGTTAGCA ACCACCAGGA CTCTGCCGAC CTGCCTAAAA TCCCACCGCC ACATACATCC 
TCAAGATCTC CAAGTCTAAA CAGATCCCTG AGAAGACAGG AGGCTATACG TATGGACTCA 
GTGGATCAGG GTGGTGATTC TAAGGACCTT AATGTGGAAT CTACAGAGAG TTTGCATCTG 
GGAGCTCCAT CAGTTACTAC TTTAGTTCCA AATTTGCTTT GCACCTTAAA TGTGTCTCTG 
CAGGGAACCC CCCAGTCCTC ACCTAGGCTA TCTCCACAGC CTAGACATTC CAGTGTCCTC 
ACACACAACC ATGTATACAG CCAACACAGT ATTCCAAGTC GGCCTTCGAG TGTCAGTAGT 
GGCAACAGCA ACCCAGAGGA ATCTATGTTT GAGTCCTCAG ACCTGGCTGA TGAAGAAGTC 
AGCCATATAA ACAGTTCAGC AAAAGACTGG AGAGGATCAT CTAATAATAG AAGTCACTCT 
GTTTCTCCAT TCATCTCCCA ATCTTCATCT CCTCCTCCTC TTTCTCCGTC ACTAAAGAAC 
TGCAATAGCA CAGTCAGCCT GGCAGGGTCC AGCGGCAAAG ATAAAGACTT GAAGAAGTTC 
TATAGTGTTG ATACTCAAGG ATTCTTAGCT AAGCCCTGCT GGGGGGATGA CCAAAGGAGG 
CACTCAATTG AGATCTGCCC TTCCATTGAT GATGGAGATT CCTGCTTTGA CCTGTCCATG 
GAGGACAAAC ATTGCTCTTC TGTTCTCATT CGGTCTGACC CCGAATACAT CAATGGAGTC 
CGACGAAAGA AGAAGATGAG TCCCCCTTGC ATTTCTATAG ACCCTCCTAT GGAGGATGAA 
GGTGGCACAG CTTCCCGTAC CAAAGCATCA GAAAACAGCA TGATGTTACG AAGAAGGACC 
CCATCCTGTG AATCTACTGC ATACAGGGAC TCTTTAGATC TTAGTGACAA CCAGCTTGGG 
GACCAAATAT CTAAAACTGA TAGACGAACC CAGGTGCCCT GTCGCAATGA ACACTTGATC 
ATACCCAGCT ACTCGTTTGA CAAGGTGGAC GGAAACGGCT TCTCAGACCT AATATCTAAC 
AGTGGGGGTT CTACTCCGAC GTCCGTTTTC AATGAACCTG TGTCTTTAGA AATGAAACAA 
AAAGATCACA ACGATCTGAG CGGCGTTTCT AAAGTTTCGT TGAAAACTGC ACTAGATCAC 
GTATCCAACC CAACAGAGGA CATAATCGAT AACCCATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952743.1
CDS	1..7542
Translation	MVRVFRQSDPVLAGSNAVEIWNRPDQSDPVLVGSNAVEMLDPARPIRSSTGDKHGDCQGEKDGNCQGDKDGDCQGDKNGDCQGDKDGDCQGYKDGDCQGAKMATAKETKMATAKGTKMATAKGTKMATAKGTKMATAKASLTLCSNQTPRTICLNLPFPTWFEHVSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKCKRRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSSPEICDVEMKIMKPKNQLRLPPASPSLQSMSPNSEAVHSIYHADCHLEAPQRKSSKSSNASSNIKLNAGLNHSNMNYPTIMPSQANKTSPATLPKGKRNGSNAVSLLSSPVSMKADSFGKTNHLVGEHGLCRSSSKLSGLSVPCSLPSPQPSPLLCEIQNCPYCANLLENPDLELSDSESCESDSNGVYEFTQDLRRGDQRDQVQKGRKLKTKPKEANKVYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTEEDKASGNFDEDLEKLKELRAAELKMYSLAVTPNGHLDPRSSMPPPIIMRTAATPMPTPKSSPCMDSSHPFGDSRRESSVSIDPSIVDQKSLASLRSTPCTPWGTYSNWGSRRSSWNSLGRAPSIKKKSQSGERESLLSGEGKGSTDDDSDDCKSSPAARVSLHRRAGSLDTRGSLDIPDFLQVPTLRHSLSVSPLAMVPSDYQDCNGKMMHVPSDLFVRVEKHREDPLEYEDDIEDSYCFRIRKMLEPYKPTWCKTHEDWSLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRRPYYAEYSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNSDDKNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEMSRALTPISGGCTPASDGKGTEGGQDPLKGELLKQVGGAVCQENVTLVIPESMEGAALLNDAVPACSIYQQQALPLPLQPCVLGHEDGDFQRRDGSGSRNLLAVKKISVSRMLSLPNDSYMFRPVLPAKSSYPLQEVETTEDNSPHGSTASLHSQPGDNNSLLNIPVECQPQASVSNHQDSADLPKIPPPHTSSRSPSLNRSLRRQEAIRMDSVDQGGDSKDLNVESTESLHLGAPSVTTLVPNLLCTLNVSLQGTPQSSPRLSPQPRHSSVLTHNHVYSQHSIPSRPSSVSSGNSNPEESMFESSDLADEEVSHINSSAKDWRGSSNNRSHSVSPFISQSSSPPPLSPSLKNCNSTVSLAGSSGKDKDLKKFYSVDTQGFLAKPCWGDDQRRHSIEICPSIDDGDSCFDLSMEDKHCSSVLIRSDPEYINGVRRKKKMSPPCISIDPPMEDEGGTASRTKASENSMMLRRRTPSCESTAYRDSLDLSDNQLGDQISKTDRRTQVPCRNEHLIIPSYSFDKVDGNGFSDLISNSGGSTPTSVFNEPVSLEMKQKDHNDLSGVSKVSLKTALDHVSNPTEDIIDNPL

Target ORF information:

RefSeq Version	XM_018097254.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, T type, alpha 1H subunit (cacna1h), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097254.1

ORF Insert Sequence:

ATGGTCCGCG TGTTCCGCCA GTCCGATCCA GTACTGGCAG GGTCCAATGC TGTGGAAATA 
TGGAACCGGC CAGACCAGTC CGATCCAGTA CTGGTAGGGT CCAATGCAGT GGAAATGTTG 
GACCCAGCCA GGCCAATCCG ATCCAGTACT GGAGATAAAC ATGGCGACTG CCAAGGGGAA 
AAAGATGGCA ACTGCCAAGG AGACAAAGAT GGCGACTGCC AAGGGGACAA AAATGGCGAC 
TGCCAAGGGG ACAAAGATGG CGACTGCCAA GGGTACAAAG ATGGCGACTG CCAAGGGGCA 
AAGATGGCTA CTGCCAAGGA GACAAAGATG GCGACTGCCA AGGGGACAAA GATGGCGACT 
GCCAAGGGGA CAAAGATGGC GACTGCCAAG GGGACAAAGA TGGCGACTGC CAAGGCATCA 
CTTACTCTAT GTTCCAACCA AACTCCAAGA ACTATTTGCC TTAACTTACC CTTTCCTACA 
TGGTTCGAGC ATGTCAGCAT GCTGGTCATT TTGCTGAACT GCGTCACCCT GGGGATGTTC 
CAGCCCTGCG AGGATACAAA CTGTATGTCA GAGAGATGCA ACATTCTAGA GGCATTCGAC 
GGCTTCATCT TTGCATTCTT TGCTGTGGAG ATGGTTATTA AGATGGTAGC GCTTGGCATC 
TTTGGGCAGA AGTGCTACCT GGGAGACACA TGGAACTGGC TGGATTTCTT CATTGTTATG 
GCAGGGATGT TGGAATATTC CCTCGATGGA CACAATGTCA GCCTATCCGC TATCCGCACT 
GTACGTGTTT TAAGGCCGCT AAGAGCCATT AACAGGGTTC CCAGTATGAG GATACTGGTG 
ACCCTGCTGC TAGATACTCT GCCTATGTTA GGCAACGTCC TTCTCTTGTG TTTCTTTGTG 
TTTTTCATCT TTGGAATCGT CGGCGTTCAG CTTTGGGCTG GGCTACTGCG CAACAGGTGT 
TTTATGGATA CAAATTTTGT AATGAGTTAC AATTTCACCC CTCTGAGGCC GTATTACCAG 
GTGGATGATG CAGAGGACAA CCCTTATATC TGCTCCTCTA ACCGAGACAA TGGGATGTTA 
AAGTGCCAGG ACATCCCTCC CAAAATGGAC TGCTCCCATG TATACTACTC AGATTTCCCC 
AGTAGGAATA GCTGCGTGAA TATTAATCAA TACTACAACG TATGCAAGCC GGGAGAGTTC 
AATCCACATA AAGGGGCCAT TAATTTTGAT AACATTGGCT ACGCCTGGAT AGCCATTTTT 
CAGGTTATAA CCCTAGAAGG ATGGGTTGAT ATCATGTACT ATGTTATGGA CGCACATTCT 
TTTTACAATT TTATATATTT TATACTACTT ATAATAGTGG GTTCTTTCTT CATGATAAAC 
TTGTGCCTGG TTGTGATAGC AACACAGTTT TCTGAAACAA AGCAGCGAGA GAACCAGCTA 
ATGCAGGAGC AACGGGCTCG TTACCTCTCC AACGACAGCA CAATCGCCAG CTTCTCGGAG 
CCAGGGAGCT GCTATGAGGA GCTGCTCAAA TACATCTGCC ACATCTTCAG GAAGGTTAAG 
CGAAGGACGT TAAGACTTTA TAATAACTGG CAGAATAAGA GGAGGAAAAA AGTTCAGCCC 
AACAGCAACA CGAACAGTCA GGGGCGTCAC CGCAAGTGCA AGCGGCGTAT CACCTCCATT 
CATCACTTAA TACACCACCA CCATCATCAC CACCACCATC ATTACCACAT CAGCAATGGG 
AGTCCCCGGG GTCCCAGGTC TAGTCCGGAG ATCTGTGATG TGGAGATGAA AATCATGAAG 
CCAAAGAATC AACTAAGGCT TCCTCCAGCA TCCCCCAGCC TCCAAAGCAT GTCCCCCAAC 
AGTGAGGCCG TCCACAGTAT CTACCACGCG GACTGCCACT TAGAGGCGCC TCAGCGGAAA 
TCGAGCAAGT CTTCCAATGC CTCGTCAAAC ATAAAACTGA ATGCTGGGTT GAATCACAGT 
AACATGAACT ATCCCACCAT CATGCCCTCC CAAGCCAACA AAACCAGCCC CGCAACACTA 
CCCAAAGGAA AAAGGAATGG CAGCAATGCA GTCAGCTTGC TAAGCAGCCC AGTCAGTATG 
AAGGCAGATT CGTTTGGGAA GACCAACCAC CTAGTCGGAG AGCATGGCCT GTGCAGGAGT 
TCCAGTAAGC TCTCCGGTTT GAGCGTGCCA TGCTCCTTGC CAAGCCCTCA GCCAAGCCCT 
CTACTGTGCG AAATCCAGAA CTGCCCGTAC TGTGCCAACC TTTTGGAGAA CCCTGACTTG 
GAGCTCAGCG ACTCTGAGAG TTGCGAATCG GATTCTAACG GGGTCTATGA ATTCACCCAG 
GACTTGAGGC GTGGAGACCA AAGAGACCAG GTGCAGAAAG GCAGAAAGCT CAAGACTAAG 
CCAAAGGAGG CTAACAAGGT GTATCTGCTC TGGGAAGAGT TTACCGTCCG ACTGAAAAGG 
ATTGTGGACA GCAAATATTT TAACCGCGGG ATTATGATTG CCATCCTGAT CAACACGCTG 
AGCATGGGGA TTGAGTATCA TGAGCAGCCA GAAGAACTGA CTAACGCCTT GGAAATCAGC 
AATATTGTGT TTACAAGCAT GTTTGCGCTG GAGATGCTGC TGAAACTCTT GGCCTTTGGG 
ATCTTTCAAT ACATCAAGAA TCCATACAAC ATCTTTGATG GGATCATTGT CGTCATCAGT 
GTGTGGGAAA TCATCGGTCA GTCGGACGGA GGTCTGTCTG TGCTTAGAAC ATTTCGCCTC 
CTTCGTGTGC TGAAGCTGGT ACGATTTATG CCAGCACTGC GGCGTCAGCT GGTGGTCCTG 
GTGAAGACGA TGGACAACGT TGCCACCTTT TGCATGCTTC TTATGCTCTT CATCTTTATA 
TTCAGCATCC TTGGCATGCA TCTGTTTGGG TGTAAGTTTA CTTGGACTGC AGACACCGGA 
GAGACAGTCA AAGACAGAAA GAACTTTGAT TCTTTGCTTT GGGCGATTGT CACCGTCTTC 
CAGATTCTCA CCCAAGAAGA CTGGAACATG GTCCTATACA ATGGCATGGC TTCCACCTCC 
TCCTGGGCAG CTCTCTACTT TGTGGCCCTG ATGACATTTG GAAATTACGT GCTTTTCAAC 
TTGTTAGTAG CCATCTTAGT CGAAGGCTTC CAAGCAGAGG GAGATGCAAC AAGGTCAGAT 
ACAGAGGAGG ACAAGGCTTC AGGCAACTTT GATGAAGATC TGGAGAAACT GAAGGAGCTT 
CGTGCTGCAG AACTGAAGAT GTACTCACTC GCAGTGACAC CCAACGGACA CCTGGACCCT 
CGGAGTTCCA TGCCCCCACC TATCATCATG CGCACCGCCG CCACCCCAAT GCCTACACCA 
AAGAGCTCCC CTTGCATGGA CTCATCACAT CCATTCGGCG ATTCAAGAAG GGAAAGTAGC 
GTCTCGATAG ATCCATCAAT CGTTGATCAG AAGTCTTTGG CAAGTCTACG CAGCACCCCT 
TGTACCCCCT GGGGCACTTA CAGCAACTGG GGAAGCCGTC GCTCTAGCTG GAATAGTCTG 
GGAAGAGCCC CAAGCATCAA AAAGAAGAGC CAATCAGGGG AGAGGGAGTC CTTACTCTCT 
GGTGAAGGAA AGGGGAGCAC AGATGATGAC TCAGATGACT GCAAGTCAAG CCCAGCGGCG 
AGGGTATCTC TCCATCGCAG GGCAGGTTCC CTGGACACGC GGGGCTCTTT GGATATCCCA 
GATTTCCTGC AGGTGCCCAC GTTACGCCAT TCCCTAAGTG TCAGTCCTTT GGCCATGGTT 
CCTTCAGATT ATCAAGATTG CAATGGGAAA ATGATGCACG TGCCCAGTGA TCTGTTCGTT 
CGGGTGGAGA AACACAGGGA GGATCCGTTG GAGTATGAAG ACGACATAGA AGATAGTTAC 
TGTTTCCGAA TCCGAAAGAT GTTGGAGCCA TACAAACCTA CATGGTGTAA GACGCATGAA 
GACTGGTCCC TCTACATGTT CTCACCTCAA AACCAGTTCC GGATGATGTG CCAAAAAGTC 
ATTGCCCACA AGATGTTTGA CCACGTGGTT CTTGTCTTCA TCTTCTTAAA CTGCATCACC 
ATTGCACTTG AACGGCCTGA TATTGACCCC AATAGCCTGG AACGGATATT CTTGAGTGTC 
TCCAATTACA TTTTTACTGC AATCTTTGTT TTGGAAATGG CAATTAAGGT GGTGGCCCTG 
GGCTTTGTCT CCGGGGAGAA CACTTACCTG CAGAGCAGCT GGAACGTGCT CGATGGGATT 
CTGGTCTTCG TTTCCATCAT CGATATCGTC GTGTCCCTTG CCTCTGCCGG GGGGGCAAAG 
ATCCTGGGAG TCTTGCGGGT ACTGAGGTTG CTGCGGACCC TGCGCCCACT TAGGGTTATC 
AGCCGAGCCC CGGGCCTGAA GTTGGTGGTA GAAACTCTCA TTTCCTCCCT GAGGCCTATC 
GGGAACATTG TCCTTATCTG CTGCGCTTTC TTCATCATCT TTGGCATTCT GGGAGTACAG 
CTTTTCAAAG GCAAGTTCTA CTACTGCGAA GGGTTAGAGA CGAGGAACAT CACCAACAGG 
TCAGAGTGCA CCCGGGCACG GTACAAATGG GTGCGGCGCA AATACAACTT TGACAACCTT 
GGCCAGGCGC TGATGTCGCT CTTTGTCCTC TCCTCCAAAG ATGGCTGGGT GAATATCATG 
TACGATGGCC TGGATGCCGT TGCCACAGAT GTTCAGCCAG TCAAAAACCA CAACCCATGG 
ATGCTCCTGT ACTTCATCTC CTTCCTCCTC ATCGTCAGCT TCTTCGTGCT CAACATGTTC 
GTAGGGGTGG TGGTAGAGAA CTTCCACAAG TGCCGGCAAC ACCAGGAGGC GGAAGAGGCC 
CGGCGCCGGG AGGAGAAACG CCTCCGCCGC CTGGAGAAAA AGCGCAGGAA GGCCCAGCGG 
AGACCGTATT ACGCAGAGTA CTCGCCGGCG CGCCTCTACA TCCACACCAT GTGTACCAGC 
CACTACCTGG ATCTCTTTAT CACCTGCATT ATTTGTATCA ACGTCATCAC CATGTCCATG 
GAACACTACA ACCAACCCAA GTCCCTGGAT GAAGCTTTAA AATACTGCAA TTACGTGTTC 
ACCATCGTCT TCGTGTTCGA GGCCCTGTTC AAGCTTGTGG CATTTGGGTT CCGGAGGTTT 
TTTAAAGACA GGTGGAACCA ACTGGACTTG GCCATCGTTT TGCTCTCCAT CATGGGAATC 
ACGTTAGAGG AGATTGAGAT TAACGCCTCA CTTCCCATCA ATCCAACTAT TATACGCATC 
ATGAGGGTGC TGAGGATTGC TCGAGTGTTA AAGCTCCTGA AAATGGCCAC TGGGATGCGA 
GCTTTGCTGG ATACTGTTGT GCAAGCGTTA CCTCAGGTAG GCAACCTTGG CCTGCTCTTC 
ATGCTCCTGT TCTTCATCTA CGCAGCCCTG GGAGTGGAGC TGTTCGGAAA GCTCGTGTGC 
AATGAGGAGA ATCCCTGTGA GGGACTCAGC CGACATGCAA CATTTGAGAA CTTTGGGATG 
GCTTTTCTGA CCCTCTTCCG TGTTTCCACA GGGGATAACT GGAATGGCAT CATGAAGGAC 
ACTCTACGAG AGTGCAACAG CGATGACAAG AACTGTCTCA CCTACCTGCC CGTCATCTCT 
CCCGTCTATT TCGTAACCTT CGTGCTCATT GCCCAGTTTG TCTTGGTCAA TGTGGTCGTG 
GCTGTGCTCA TGAAACATCT AGAGGAGAGC AATAAAGAGG CCAAAGAAGA TGCCGAGATG 
GATGCAGAGA TTGAGATGGA GATGTCTAGG GCACTGACAC CTATAAGTGG TGGCTGCACT 
CCAGCTTCAG ATGGCAAGGG AACTGAGGGA GGGCAAGATC CATTAAAGGG GGAGCTGCTG 
AAACAAGTGG GGGGGGCAGT GTGCCAAGAG AATGTGACCT TAGTGATCCC AGAGTCAATG 
GAGGGTGCTG CCCTGCTGAA CGACGCAGTC CCTGCTTGCT CCATATACCA ACAGCAGGCC 
CTGCCACTTC CCCTCCAGCC TTGCGTCCTT GGGCACGAGG ATGGGGATTT CCAAAGGAGG 
GATGGATCGG GCTCCCGAAA CCTACTGGCT GTCAAAAAGA TCTCGGTGTC TAGGATGCTC 
TCCTTACCTA ATGACAGCTA CATGTTTCGC CCAGTGCTAC CAGCAAAGTC ATCTTATCCT 
TTGCAAGAAG TGGAGACCAC TGAAGATAAT TCACCACATG GGTCGACTGC TTCACTCCAC 
TCCCAGCCAG GGGACAATAA CTCATTGCTG AATATACCAG TGGAGTGCCA GCCACAGGCC 
TCTGTTAGCA ACCACCAGGA CTCTGCCGAC CTGCCTAAAA TCCCACCGCC ACATACATCC 
TCAAGATCTC CAAGTCTAAA CAGATCCCTG AGAAGACAGG AGGCTATACG TATGGACTCA 
GTGGATCAGG GTGGTGATTC TAAGGACCTT AATGTGGAAT CTACAGAGAG TTTGCATCTG 
GGAGCTCCAT CAGTTACTAC TTTAGTTCCA AATTTGCTTT GCACCTTAAA TGTGTCTCTG 
CAGGGAACCC CCCAGTCCTC ACCTAGGCTA TCTCCACAGC CTAGACATTC CAGTGTCCTC 
ACACACAACC ATGTATACAG CCAACACAGT ATTCCAAGTC GGCCTTCGAG TGTCAGTAGT 
GGCAACAGCA ACCCAGAGGA ATCTATGTTT GAGTCCTCAG ACCTGGCTGA TGAAGAAGTC 
AGCCATATAA ACAGTTCAGC AAAAGACTGG AGAGGATCAT CTAATAATAG AAGTCACTCT 
GTTTCTCCAT TCATCTCCCA ATCTTCATCT CCTCCTCCTC TTTCTCCGTC ACTAAAGAAC 
TGCAATAGCA CAGTCAGCCT GGCAGGGTCC AGCGGCAAAG ATAAAGACTT GAAGAAGTTC 
TATAGTGTTG ATACTCAAGG ATTCTTAGCT AAGCCCTGCT GGGGGGATGA CCAAAGGAGG 
CACTCAATTG AGATCTGCCC TTCCATTGAT GATGGAGATT CCTGCTTTGA CCTGTCCATG 
GAGGACAAAC ATTGCTCTTC TGTTCTCATT CGGTCTGACC CCGAATACAT CAATGGAGTC 
CGACGAAAGA AGAAGATGAG TCCCCCTTGC ATTTCTATAG ACCCTCCTAT GGAGGATGAA 
GGTGGCACAG CTTCCCGTAC CAAAGCATCA GAAAACAGCA TGATGTTACG AAGAAGGACC 
CCATCCTGTG AATCTACTGC ATACAGGGAC TCTTTAGATC TTAGTGACAA CCAGCTTGGG 
GACCAAATAT CTAAAACTGA TAGACGAACC CAGGTGCCCT GTCGCAATGA ACACTTGATC 
ATACCCAGCT ACTCGTTTGA CAAGGTGGAC GGAAACGGCT TCTCAGACCT AATATCTAAC 
AGTGGGGGTT CTACTCCGAC GTCCGTTTTC AATGAACCTG TGTCTTTAGA AATGAAACAA 
AAAGATCACA ACGATCTGAG CGGCGTTTCT AAAGTTTCGT TGAAAACTGC ACTAGATCAC 
GTATCCAACC CAACAGAGGA CATAATCGAT AACCCATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cacna1h cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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