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hybridoma

A cell that is useful in production of quantities of a monoclonal antibody; a fusion of a myeloma cell and a spleen lymphocyte that, like the myeloma cell, can divide in culture and, like the spleen cell, can produce antibodies. The use of HAT (hypoxanthine/ aminopterin/thymidine) medium allows selection of hybrids of myeloma cell mutants that lack hypoxanthine-guanine phosphoribosyltransferase and consequently cannot utilize hypoxanthine, with spleen cells, which do not proliferate in vitro. (see also hypoxanthine/aminopterin/thymidine (HAT) medium)


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If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]

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