List by Alphabet: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

antibody purification

Antibody purification is a process used to isolate antibodies from a mixture, such as a biological fluid or a cell culture medium, in order to obtain a preparation of antibodies that are free from other proteins and contaminants. This process is crucial in both research and clinical settings, where highly pure antibodies are needed for various applications. Here's an overview of the key steps and methods involved in antibody purification:

1. Source of Antibodies:
2. Initial Sample Preparation:
3. Protein A, G, or L Affinity Chromatography:
4. Ion Exchange Chromatography:
5. Size Exclusion Chromatography (Gel Filtration):
6. Precipitation Techniques:
7. Dialysis and Buffer Exchange:
8. Assessment of Purity: Techniques such as SDS-PAGE, Western blotting, and ELISA may be used to assess the purity and concentration of the purified antibodies.

The specific method chosen for antibody purification depends on factors like the type and class of antibody, the source of the antibodies, the required purity, and the intended application (e.g., research, diagnostic, therapeutic). Each method has its advantages and limitations, and often, multiple methods are combined to achieve the desired level of purity.

Related Biology Tools

  • GenSmart™ Codon Optimization

    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

    Learn More

  • DNA Construct Design Tool

    GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.

    Learn More

  • Codon Frequency Tables

    This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.

    Learn More

Service and Products

Antibody Production Services

Comprehensive custom antibody production services including monoclonal and polyclonal antibody production services with industry leading turnaround times.

Polyclonal Antibody Services

The fastest custom polyclonal antibody production services in the industry with unmatched guarantees.

Custom Monoclonal Antibody Development Services

Developing custom monoclonal antibodies with standard or fully custom protocols including everything from antigen synthesis to antibody scale-up options.

Phospho-Specific Antibody Services

Guaranteed ELISA titer of ≥ 1:64,000 and < 10% cross reactivity with non-phospho peptide.

Immunoassay Development Services

Our custom immunoassay development service offering comprehensive high-throughput assay development with fast turnaround and high efficiency.

Large-scale Antibody Manufacturing

Our large-scale antibody manufacturing services offer polyclonal and monoclonal antibody production of gram quantities for industrial antibody yields.

COVID-19 Reagent Antibodies

On demand SARS-CoV-2 control mAbs ideal for diagnostics, therapeutic, and vaccine development.

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]

If your term is adopted, we will send 1,000 EzCoupon points to your GenScript account.