CRISPR Non-viral CAR-T Knock-In Optimization Kits

Choose your desired gene target insertion site:

• TRAC – Mostly commonly used site for new CAR insertion in T cells, our sgRNA is designed to target the first exon on the TRAC locus
• RAB11A – Housekeeping gene for more general insertion optimization
• Other Gene Target – Check out our customized sgRNA and ssDNA/dsDNA Services

Choose your Cas9 protein:

• Wild type Cas9 protein – The research standard for gene editing with one N-terminal nuclear localization signal (NLS).
• Enhanced specificity SpCas9 (eSpCas9)-2NLS protein – SpCas9 (K848A/K1003A/R1060A) is structurally engineered with double-ended nuclear localization signals (NLSs) for improved target specificity by Feng Zhang Laboratory at the Broad Institute ¹. eSpCas9 is able to reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency.

Choose your DNA template:

• GenExact™ ssDNA only
• GenWand™ dsDNA only
• GenExact ssDNA and GenWand dsDNA – ideal solution for optimizing HDR DNA templates

Would you like to include the Cas9 Targeting Sequence (CTS) in your DNA template design for improved knock-in efficiency?
See FAQ section below for more details*

• Yes – Selecting this will add donor templates with CTS design to your test kits, as well as corresponding oligo for the CTS annealing
• No