MYH1F cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH1F
Entrez Gene ID	768566
Full Name	myosin, heavy chain 1F, skeletal muscle
General protein information	Preferred Names myosin, heavy chain 1F, skeletal muscle Names myosin, heavy chain 1F, skeletal muscle myosin-3 myosin, heavy chain 1F, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) myosin, heavy chain 1F, skeletal muscle; myosin-3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001319015.1	NP_001305944.1	myosin, heavy chain 1F, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult)
XM_001231408.4	XP_001231409.1	myosin, heavy chain 1F, skeletal muscle isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MYH1C	NP_001107181.1
Mus musculus (house mouse)	Myh2	NP_001034634.2
Gallus gallus (chicken)	MYH1D	XP_415578.4
Gallus gallus (chicken)	MYH1E	NP_001013415.1
Bos taurus (cattle)	MYH2	NP_001159699.1
	MYH2	XP_002800336.1
Canis lupus familiaris (dog)	MYH2	NP_001070263.1
Gallus gallus (chicken)	MYH1A	NP_001013414.1
Gallus gallus (chicken)	MYH1F	XP_001231409.1
Homo sapiens (human)	MYH2	NP_001093582.1
Rattus norvegicus (Norway rat)	Myh2	NP_001128629.1
Gallus gallus (chicken)	MYH1B	NP_989559.1
Gallus gallus (chicken)	MYH1G	XP_003642358.1

Gene Ontology
Bibliography

Related articles in PubMed

Evidence for the expression of neonatal skeletal myosin heavy chain in primary myocardium and cardiac conduction tissue in the developing chick heart.
Machida S, Matsuoka R, Noda S, Hiratsuka E, Takagaki Y, Oana S, Furutani Y, Nakajima H, Takao A, Momma K
Developmental dynamics : an official publication of the American Association of Anatomists217(1)37-49(2000 Jan)

Analysis of the chicken fast myosin heavy chain family. Localization of isoform-specific antibody epitopes and regions of divergence.
Moore LA, Arrizubieta MJ, Tidyman WE, Herman LA, Bandman E
Journal of molecular biology225(4)1143-51(1992 Jun)

A canonical sequence organization at the 5'-end of the myosin heavy chain genes.
Kropp K, Gulick J, Robbins J
The Journal of biological chemistry261(14)6613-8(1986 May)

The chicken myosin heavy chain family.
Robbins J, Horan T, Gulick J, Kropp K
The Journal of biological chemistry261(14)6606-12(1986 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYH1F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH1F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa86397	NM_001319015.1 Latest version!	Gallus gallus myosin, heavy chain 1F, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa16447	XM_001231408.4 Latest version!	Gallus gallus myosin, heavy chain 1F, skeletal muscle (MYH1F), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH1F ( NM_001319015.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001305944.1 Gallus gallus myosin, heavy chain 1F, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1F), mRNA.

Price & Availability↑

CloneID	OGa86397
Clone ID Related Accession (Same CDS sequence)	NM_001319015.1
Accession Version	NM_001319015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Gallus gallus(chicken)
Product	myosin, heavy chain 1F, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult)
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB021180.1. ##Evidence-Data-START## Transcript exon combination :: AB021180.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCATCAG ATGCTGAGAT GGCCATATTT GGGGAGGCAG CCCCTTACCT CCGAAAGTCA 
GAAAAGGAGA GGATTGAGGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTACATGCTA AAGAATCCTA TGTGAAAAGT ACAATCCAGA GTAAAGAATC GGGGAAGGTC 
ACTGTCAAGA CTGAAAGTGG AGAAACTCTA ACCGTGAAGG AAGATCAAAT CTTCTCCATG 
AACCCTCCCA AGTATGATAA AATTGAGGAC ATGGCCATGA TGACCCACCT CCACGAACCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC GGAGGTGGTG 
TTGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCTCCAC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGC GAGAATCAGT CGATCCTGAT CACCGGAGAA 
TCCGGTGCAG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GCTAGTGGAG ATAAGAAGAA GGAGGAGCAG CCAGCGGGCA AAATGCAGGG GACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCTCTGTTG GAGGCTTTTG GGAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ACTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAGAAGT CTAGGGTCAC TTTCCAGCTC 
AAGGCTGAAA GAAGCTACCA CATCTTTTAT CAGATCATGT CCAACAAGAA GCCAGAGCTA 
ATTGAGATGC TGCTGATCAC CACCAACCCA TACGACTATC ACTATGTGAG TCAAGGCGAG 
ATCACAGTGC CAAGCATTAA TGACCAGGAG GAGCTGATGG CCACCGATAG TGCCATTGAC 
ATCCTGGGCT TCACTCCTGA TGAAAAAACA GCCATTTACA AGCTGACGGG GGCTGTCATG 
CACTATGGCA ACCTGAAGTT CAAGCAGAAG CAGCGTGAAG AGCAGGCAGA GCCCGATGGC 
ACAGAAGTTG CTGACAAGGC TGCTTACCTG ATGGGTCTCA ACTCAGCAGA CCTGCTCAAG 
GCCCTGTGCT ACCCCCGAGT CAAAGTTGGG AATGAATTTG TGACCAAGGG TCAGACTGTG 
CAGCAGGTGT ACAACTCAGT TGGTGCCCTG GCAAAGGCTG TCTTTGAGAA GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAACAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGTCTGGA ACAGCTGTGC 
ATCAACTTCA CGAATGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT TGTGCTGGAA 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGGAGTTCA TTGACTTTGG GATGGACTTG 
GCTGCCTGCA TTGAGCTCAT TGAAAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA ACAACTTCCA GAAGCCTAAA CCTGGCAAAG GCAAGGCTGA GGCCCACTTC 
TCCCTGGTGC ACTATGCTGG CACAGTGGAC TACAACATCT CTGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGTATGAAAC TGTTGTGGGG CTGTACCAAA AATCATCCCT GAAGACACTG 
GCCTTACTGT TTGCTTCTGC TGGCGGAGAG GCAGAGAGTG GTGGTGGTGG CAAGAAGGGA 
GGCAAGAAGA AGGGTTCTTC TTTCCAGACT GTATCAGCTC TTTTCAGGGA GAACCTGAAC 
AAGCTGATGA CCAACCTACG GAGCACTCAC CCCCATTTTG TGCGCTGTAT CATCCCTAAT 
GAAACAAAAA CTCCCGGTGC CATGGAGCAT GAACTGGTGC TGCACCAGCT GCGTTGTAAT 
GGTGTGCTAG AAGGAATCAG AATTTGCAGA AAGGGATTCC CCAGCAGAAT CCTCTATGCA 
GACTTCAAAC AGAGATACAA GGTGCTTAAT GCCAGTGCCA TCCCTGAGGG ACAGTTCATT 
GACAGCAAGA AAGCTTCTGA GAAGCTACTT GGGTCAATCG ATGTGGACCA CACCCAGTAC 
AAATTTGGTC ACACCAAGGT GTTCTTCAAA GCTGGGCTGC TGGGACTTCT GGAGGAGATG 
AGGGATGAGA AGCTAGCACA GCTCATCACT CGCACACAGG CCAGGTGCAG GGGCTTCCTG 
ATGAGAGTGG AGTACCGCAG AATGGTGGAG AGGAGAGAAT CCATCTTCTG CATCCAGTAC 
AATATTCGTT CATTCATGAA TGTCAAGCAC TGGCCCTGGA TGAAGCTGTT CTTTAAGATC 
AAGCCGTTGC TGAAGAGCGC AGAGTCTGAG AAGGAAATGG CCAACATGAA AGGGGAGTTT 
GAGAAGACCA AGGAAGAACT TGCAAAGTCT GGGGCAAAGA GGAAAGATTT GGAGGGAAAA 
ATGGTGTCCC TGCTGCAGGA GAAAAATGAC TTGCAGCTCC AAGTCCAGGC TGAAGCAGAT 
GCCTTAGCTG ATGCTGAGGA AAGGTGTGAC CAGCTCATCA AAACCAAAAT CCAGCTGGAA 
GCCAAAATTA AGGAGGTGAC AGAACGGGCT GAGGATGAGG AGGAAATTAA TGCTGAACTG 
ACGGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAGC TGAAGAAAGA CATAGATGAC 
CTGGAGTTAA CACTGGCCAA AGTTGAGAAG GAAAAGCATG CTACTGAAAA CAAGGTGAAA 
AACCTCACAG AGGAGATGGC AGCCTTGGAC GAGAACATTG CCAAGCTGAC AAAAGAGAAG 
AAAGCCCCCC AAGAGGCCCA TCAGCAGACA CTGGATGACC TGCAGGCTGA AGAGGACAAA 
GTCAATACGC TGACCAAAGC TAAAACAAAG CTGGAGCAGC AAGTGGACGA TCTGGAAGGG 
TCCCTGGAGC AAGAGAAGAA GCTGCGCATG GACCTCGAGA GAGCTAAGAG GAAACTTGAA 
GGAGACCTGA AGTTGGCCCA TGATAGCATA ATGGACTTGG AAAATGATAA ACAGCAGCTG 
GATGAGAAAC TGAAGAAGAA AGACTTTGAA ATCAGCCAGA TCCAGAGCAA AATTGAGGAT 
GAGCAAGCCC TGGGCATGCA GTTACAGAAG AAGATCAAGG AGCTGCAGGC CCGTACTGAG 
GAACTGGAGG AGGAAATCGA AGCAGAGCGA ACCTCTCGGG CAAAAGCAGA GAAGCACCGA 
GCTGACCTCT CCAGGGAGCT GGAGGAGATC AGTGAGCGCC TGGAGGAAGC AGGAGGGGCT 
ACAGCAGCTC AGATTGATAT GAACAAGAAG CGTGAGGCAG AATTTCAGAA GATGCGCCGT 
GACCTCGAAG AGGCCACGCT GCAGCACGAA GCCACGGCTG CTGCCCTGCG GAAGAAGCAT 
GCGGACAGCA CAGCAGAGCT TGGGGAGCAG ATCGACAACC TGCAACGAGT CAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GCTGAAGATG GAGATTGATG ACTTGGCCAG CAACATGGAG 
TCTGTCTCCA AAGCGAAGGC AAGTCTGGAG AAGACATGCC GAGCATTGGA AGATCAGATG 
AGTGAGATTA AGACAAAAGA AGAGGAGCAT CAACGGATGA TCAATGATGT TAATGCTCAA 
AGAGCTCGTC TGCAGACAGA ATCAGGTGAA TATTCACGCC AGGTGGAGGA GAAAGATGCT 
CTGATTTCTC AGCTGTCTAG AGGCAAGCAG GCATTCACCC AACAGATTGA GGAACTCAAG 
AGGCACTTAG AGGAGGAGAT AAAGGCCAAG AATGCCCTAG CCCATGGTTT GCAGTCTGCT 
CGCCATGACT GTGATTTGCT CCGGGAACAA TATGAGGAGG AGCAGGAAGC CAAGGGTGAG 
CTGCAGCGTG CCCTGTCCAA GGCCAACAGC GAAGTGGCCC AGTGGAGAAC CAAATATGAG 
ACGGATGCTA TTCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCACAGCGC 
CTGCAAGATG CTGAGGAACA CGTCGAAGCC GTGAACTCCA AATGTGCTTC CCTGGAAAAG 
ACAAAGCAGA GGCTGCAGAA TGAGGTGGAG GACCTGATGA TTGACGTGGA GCGAGCAAAT 
TCTGCCTGCG CAGCTCTGGA CAAGAAGCAG AAGAACTTTG ACAAGATCCT GTCGGAGTGG 
AAGCAGAAGT ACGAGGAAAC GCAGGCTGAG CTGGAGGCCT CCCAGAAGGA GTCTCGCTCC 
CTCAGCACAG AGCTGTTTAA GATGAAGAAT GCCTATGAGG AATCCCTGGA CCACCTGGAA 
ACACTGAAGC GTGAGAACAA GAACTTGCAG CAGGAGATTT CTGACCTCAC GGAGCAGATT 
GCAGAGGGAG GAAAGGCTAT TCATGAGCTG GAGAAAGTCA AGAAGCAGAT TGAGCAGGAG 
AAATCTGAAC TTCAAGCCTC CCTAGAGGAA GCAGAAGCCT CCCTGGAACA TGAAGAAGGG 
AAGATCCTGC GCCTTCAACT GGAGCTCAAC CAGGTCAAGT CTGAGATTGA CAGGAAGATA 
GCAGAGAAAG ATGAGGAAAT TGACCAGCTG AAGAGAAACC ATCTCAGAAT TGTGGAGTCC 
ATGCAGCGCA CTCTGGATGC TGAGGTCAGG AGCAGGAATG AAGCCCTGAG GCTGAAGAAG 
AAGATGGAGG GAGACCTGAA TGAAATGGAG ATCCAGCTCA ACCATGCCAA CCGCATGGCT 
GCAGAGGCAC AAAAGAACCT GAGAAACACA CAAGGAGTGC TCAAGGATAC CCAGATACAC 
TTGGACGATG CTCTCAGGTC ACAGGAAGAC CTGAAGGAGC AGGTGGCCAT GGTGGAGCGC 
AGAGCAAACC TGTTGCAGGC TGAAACTGAA GAGCTACGGG CAGCGCTGGA GCAGACAGAG 
CGCTCGAGGA AAGTGGCTGA GCAGGAGCTT CTGGATGCAA GTGAACGTGT GCAGCTCCTC 
CATACCCAGA ACACCAGCTT GATCAACACC AAGAAGAAGC TAGAATCAGA CATTTCCCAA 
ATTCAGAGTG AAATGGAGGA TACCATCCAG GAAGCCCGCA ATGCTGAGGA GAAGGCCAAG 
AAGGCCATCA CCGATGCAGC CATGATGGCA GAAGAGCTGA AGAAGGAGCA GGACACCAGT 
GCCCACCTGG AGAGGATGAA GAAGAACCTG GACCAGACGG TGAAGGACCT GCAGCACCGC 
CTGGATGAGG CAGAGCAGCT GGCCCTGAAG GGAGGCAAGA AGCAAATCCA GAAGCTGGAG 
GCCAGGGTGC GGGAGCTGGA AGGGGAGGTT GATGCTGAGC AGAAGCGCAG CGCTGAAGCC 
GTGAAGGGTG TACGCAAGTA CGAGAGGAGG GTGAAGGAGC TGACCTACCA GTCTGAAGAA 
GACCGGAAGA ATGTTCTCAG GCTCCAGGAC CTGGTGGACA AGCTGCAGAT GAAGGTGAAA 
TCCTACAAGA GGCAGGCTGA GGAGGCTGAG GAGCTGTCCA ATGTCAACCT TTCCAAATTC 
CGCAAGATCC AGCACGAGCT GGAGGAAGCC GAGGAGCGGG CTGACATTGC AGAGTCACAG 
GTCAACAAGC TCCGAGTGAA GAGTCGTGAG TTTCATAAGA AGATAGAAGA GGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305944.1
CDS	1..5820
Translation	MSSDAEMAIFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVVHAKESYVKSTIQSKESGKVTVKTESGETLTVKEDQIFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGDKKKEEQPAGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDYHYVSQGEITVPSINDQEELMATDSAIDILGFTPDEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADLLKALCYPRVKVGNEFVTKGQTVQQVYNSVGALAKAVFEKMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKPGKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLYETVVGLYQKSSLKTLALLFASAGGEAESGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLMRVEYRRMVERRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKGEFEKTKEELAKSGAKRKDLEGKMVSLLQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDENIAKLTKEKKAPQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQALGMQLQKKIKELQARTEELEEEIEAERTSRAKAEKHRADLSRELEEISERLEEAGGATAAQIDMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKASLEKTCRALEDQMSEIKTKEEEHQRMINDVNAQRARLQTESGEYSRQVEEKDALISQLSRGKQAFTQQIEELKRHLEEEIKAKNALAHGLQSARHDCDLLREQYEEEQEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERANSACAALDKKQKNFDKILSEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSELQASLEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESMQRTLDAEVRSRNEALRLKKKMEGDLNEMEIQLNHANRMAAEAQKNLRNTQGVLKDTQIHLDDALRSQEDLKEQVAMVERRANLLQAETEELRAALEQTERSRKVAEQELLDASERVQLLHTQNTSLINTKKKLESDISQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQMKVKSYKRQAEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRVKSREFHKKIEEEE

Target ORF information:

RefSeq Version	NM_001319015.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1F, skeletal muscle (similar to human myosin, heavy chain 1, skeletal muscle, adult) (MYH1F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319015.1

ORF Insert Sequence:

ATGTCATCAG ATGCTGAGAT GGCCATATTT GGGGAGGCAG CCCCTTACCT CCGAAAGTCA 
GAAAAGGAGA GGATTGAGGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTACATGCTA AAGAATCCTA TGTGAAAAGT ACAATCCAGA GTAAAGAATC GGGGAAGGTC 
ACTGTCAAGA CTGAAAGTGG AGAAACTCTA ACCGTGAAGG AAGATCAAAT CTTCTCCATG 
AACCCTCCCA AGTATGATAA AATTGAGGAC ATGGCCATGA TGACCCACCT CCACGAACCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC GGAGGTGGTG 
TTGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCTCCAC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGC GAGAATCAGT CGATCCTGAT CACCGGAGAA 
TCCGGTGCAG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GCTAGTGGAG ATAAGAAGAA GGAGGAGCAG CCAGCGGGCA AAATGCAGGG GACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCTCTGTTG GAGGCTTTTG GGAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ACTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAGAAGT CTAGGGTCAC TTTCCAGCTC 
AAGGCTGAAA GAAGCTACCA CATCTTTTAT CAGATCATGT CCAACAAGAA GCCAGAGCTA 
ATTGAGATGC TGCTGATCAC CACCAACCCA TACGACTATC ACTATGTGAG TCAAGGCGAG 
ATCACAGTGC CAAGCATTAA TGACCAGGAG GAGCTGATGG CCACCGATAG TGCCATTGAC 
ATCCTGGGCT TCACTCCTGA TGAAAAAACA GCCATTTACA AGCTGACGGG GGCTGTCATG 
CACTATGGCA ACCTGAAGTT CAAGCAGAAG CAGCGTGAAG AGCAGGCAGA GCCCGATGGC 
ACAGAAGTTG CTGACAAGGC TGCTTACCTG ATGGGTCTCA ACTCAGCAGA CCTGCTCAAG 
GCCCTGTGCT ACCCCCGAGT CAAAGTTGGG AATGAATTTG TGACCAAGGG TCAGACTGTG 
CAGCAGGTGT ACAACTCAGT TGGTGCCCTG GCAAAGGCTG TCTTTGAGAA GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAACAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGTCTGGA ACAGCTGTGC 
ATCAACTTCA CGAATGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT TGTGCTGGAA 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGGAGTTCA TTGACTTTGG GATGGACTTG 
GCTGCCTGCA TTGAGCTCAT TGAAAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA ACAACTTCCA GAAGCCTAAA CCTGGCAAAG GCAAGGCTGA GGCCCACTTC 
TCCCTGGTGC ACTATGCTGG CACAGTGGAC TACAACATCT CTGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGTATGAAAC TGTTGTGGGG CTGTACCAAA AATCATCCCT GAAGACACTG 
GCCTTACTGT TTGCTTCTGC TGGCGGAGAG GCAGAGAGTG GTGGTGGTGG CAAGAAGGGA 
GGCAAGAAGA AGGGTTCTTC TTTCCAGACT GTATCAGCTC TTTTCAGGGA GAACCTGAAC 
AAGCTGATGA CCAACCTACG GAGCACTCAC CCCCATTTTG TGCGCTGTAT CATCCCTAAT 
GAAACAAAAA CTCCCGGTGC CATGGAGCAT GAACTGGTGC TGCACCAGCT GCGTTGTAAT 
GGTGTGCTAG AAGGAATCAG AATTTGCAGA AAGGGATTCC CCAGCAGAAT CCTCTATGCA 
GACTTCAAAC AGAGATACAA GGTGCTTAAT GCCAGTGCCA TCCCTGAGGG ACAGTTCATT 
GACAGCAAGA AAGCTTCTGA GAAGCTACTT GGGTCAATCG ATGTGGACCA CACCCAGTAC 
AAATTTGGTC ACACCAAGGT GTTCTTCAAA GCTGGGCTGC TGGGACTTCT GGAGGAGATG 
AGGGATGAGA AGCTAGCACA GCTCATCACT CGCACACAGG CCAGGTGCAG GGGCTTCCTG 
ATGAGAGTGG AGTACCGCAG AATGGTGGAG AGGAGAGAAT CCATCTTCTG CATCCAGTAC 
AATATTCGTT CATTCATGAA TGTCAAGCAC TGGCCCTGGA TGAAGCTGTT CTTTAAGATC 
AAGCCGTTGC TGAAGAGCGC AGAGTCTGAG AAGGAAATGG CCAACATGAA AGGGGAGTTT 
GAGAAGACCA AGGAAGAACT TGCAAAGTCT GGGGCAAAGA GGAAAGATTT GGAGGGAAAA 
ATGGTGTCCC TGCTGCAGGA GAAAAATGAC TTGCAGCTCC AAGTCCAGGC TGAAGCAGAT 
GCCTTAGCTG ATGCTGAGGA AAGGTGTGAC CAGCTCATCA AAACCAAAAT CCAGCTGGAA 
GCCAAAATTA AGGAGGTGAC AGAACGGGCT GAGGATGAGG AGGAAATTAA TGCTGAACTG 
ACGGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAGC TGAAGAAAGA CATAGATGAC 
CTGGAGTTAA CACTGGCCAA AGTTGAGAAG GAAAAGCATG CTACTGAAAA CAAGGTGAAA 
AACCTCACAG AGGAGATGGC AGCCTTGGAC GAGAACATTG CCAAGCTGAC AAAAGAGAAG 
AAAGCCCCCC AAGAGGCCCA TCAGCAGACA CTGGATGACC TGCAGGCTGA AGAGGACAAA 
GTCAATACGC TGACCAAAGC TAAAACAAAG CTGGAGCAGC AAGTGGACGA TCTGGAAGGG 
TCCCTGGAGC AAGAGAAGAA GCTGCGCATG GACCTCGAGA GAGCTAAGAG GAAACTTGAA 
GGAGACCTGA AGTTGGCCCA TGATAGCATA ATGGACTTGG AAAATGATAA ACAGCAGCTG 
GATGAGAAAC TGAAGAAGAA AGACTTTGAA ATCAGCCAGA TCCAGAGCAA AATTGAGGAT 
GAGCAAGCCC TGGGCATGCA GTTACAGAAG AAGATCAAGG AGCTGCAGGC CCGTACTGAG 
GAACTGGAGG AGGAAATCGA AGCAGAGCGA ACCTCTCGGG CAAAAGCAGA GAAGCACCGA 
GCTGACCTCT CCAGGGAGCT GGAGGAGATC AGTGAGCGCC TGGAGGAAGC AGGAGGGGCT 
ACAGCAGCTC AGATTGATAT GAACAAGAAG CGTGAGGCAG AATTTCAGAA GATGCGCCGT 
GACCTCGAAG AGGCCACGCT GCAGCACGAA GCCACGGCTG CTGCCCTGCG GAAGAAGCAT 
GCGGACAGCA CAGCAGAGCT TGGGGAGCAG ATCGACAACC TGCAACGAGT CAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GCTGAAGATG GAGATTGATG ACTTGGCCAG CAACATGGAG 
TCTGTCTCCA AAGCGAAGGC AAGTCTGGAG AAGACATGCC GAGCATTGGA AGATCAGATG 
AGTGAGATTA AGACAAAAGA AGAGGAGCAT CAACGGATGA TCAATGATGT TAATGCTCAA 
AGAGCTCGTC TGCAGACAGA ATCAGGTGAA TATTCACGCC AGGTGGAGGA GAAAGATGCT 
CTGATTTCTC AGCTGTCTAG AGGCAAGCAG GCATTCACCC AACAGATTGA GGAACTCAAG 
AGGCACTTAG AGGAGGAGAT AAAGGCCAAG AATGCCCTAG CCCATGGTTT GCAGTCTGCT 
CGCCATGACT GTGATTTGCT CCGGGAACAA TATGAGGAGG AGCAGGAAGC CAAGGGTGAG 
CTGCAGCGTG CCCTGTCCAA GGCCAACAGC GAAGTGGCCC AGTGGAGAAC CAAATATGAG 
ACGGATGCTA TTCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCACAGCGC 
CTGCAAGATG CTGAGGAACA CGTCGAAGCC GTGAACTCCA AATGTGCTTC CCTGGAAAAG 
ACAAAGCAGA GGCTGCAGAA TGAGGTGGAG GACCTGATGA TTGACGTGGA GCGAGCAAAT 
TCTGCCTGCG CAGCTCTGGA CAAGAAGCAG AAGAACTTTG ACAAGATCCT GTCGGAGTGG 
AAGCAGAAGT ACGAGGAAAC GCAGGCTGAG CTGGAGGCCT CCCAGAAGGA GTCTCGCTCC 
CTCAGCACAG AGCTGTTTAA GATGAAGAAT GCCTATGAGG AATCCCTGGA CCACCTGGAA 
ACACTGAAGC GTGAGAACAA GAACTTGCAG CAGGAGATTT CTGACCTCAC GGAGCAGATT 
GCAGAGGGAG GAAAGGCTAT TCATGAGCTG GAGAAAGTCA AGAAGCAGAT TGAGCAGGAG 
AAATCTGAAC TTCAAGCCTC CCTAGAGGAA GCAGAAGCCT CCCTGGAACA TGAAGAAGGG 
AAGATCCTGC GCCTTCAACT GGAGCTCAAC CAGGTCAAGT CTGAGATTGA CAGGAAGATA 
GCAGAGAAAG ATGAGGAAAT TGACCAGCTG AAGAGAAACC ATCTCAGAAT TGTGGAGTCC 
ATGCAGCGCA CTCTGGATGC TGAGGTCAGG AGCAGGAATG AAGCCCTGAG GCTGAAGAAG 
AAGATGGAGG GAGACCTGAA TGAAATGGAG ATCCAGCTCA ACCATGCCAA CCGCATGGCT 
GCAGAGGCAC AAAAGAACCT GAGAAACACA CAAGGAGTGC TCAAGGATAC CCAGATACAC 
TTGGACGATG CTCTCAGGTC ACAGGAAGAC CTGAAGGAGC AGGTGGCCAT GGTGGAGCGC 
AGAGCAAACC TGTTGCAGGC TGAAACTGAA GAGCTACGGG CAGCGCTGGA GCAGACAGAG 
CGCTCGAGGA AAGTGGCTGA GCAGGAGCTT CTGGATGCAA GTGAACGTGT GCAGCTCCTC 
CATACCCAGA ACACCAGCTT GATCAACACC AAGAAGAAGC TAGAATCAGA CATTTCCCAA 
ATTCAGAGTG AAATGGAGGA TACCATCCAG GAAGCCCGCA ATGCTGAGGA GAAGGCCAAG 
AAGGCCATCA CCGATGCAGC CATGATGGCA GAAGAGCTGA AGAAGGAGCA GGACACCAGT 
GCCCACCTGG AGAGGATGAA GAAGAACCTG GACCAGACGG TGAAGGACCT GCAGCACCGC 
CTGGATGAGG CAGAGCAGCT GGCCCTGAAG GGAGGCAAGA AGCAAATCCA GAAGCTGGAG 
GCCAGGGTGC GGGAGCTGGA AGGGGAGGTT GATGCTGAGC AGAAGCGCAG CGCTGAAGCC 
GTGAAGGGTG TACGCAAGTA CGAGAGGAGG GTGAAGGAGC TGACCTACCA GTCTGAAGAA 
GACCGGAAGA ATGTTCTCAG GCTCCAGGAC CTGGTGGACA AGCTGCAGAT GAAGGTGAAA 
TCCTACAAGA GGCAGGCTGA GGAGGCTGAG GAGCTGTCCA ATGTCAACCT TTCCAAATTC 
CGCAAGATCC AGCACGAGCT GGAGGAAGCC GAGGAGCGGG CTGACATTGC AGAGTCACAG 
GTCAACAAGC TCCGAGTGAA GAGTCGTGAG TTTCATAAGA AGATAGAAGA GGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1F ( XM_001231408.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_001231409.1 Gallus gallus myosin, heavy chain 1F, skeletal muscle (MYH1F), transcript variant X1, mRNA.

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CloneID	OGa16447
Clone ID Related Accession (Same CDS sequence)	XM_001231408.4
Accession Version	XM_001231408.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	myosin, heavy chain 1F, skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_001231408.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAG ATGCTGAGAT GGCCATATTT GGGGAGGCAG CCCCTTACCT CCGAAAGTCA 
GAAAAGGAGA GGATTGAGGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTACATGCTA AAGAATCCTA TGTGAAAAGT ACAATCCAGA GTAAAGAATC GGGGAAGGTC 
ACTGTCAAGA CTGAAAGTGG AGAAACTCTA ACCGTGAAGG AAGATCAAAT CTTCTCCATG 
AACCCTCCCA AGTATGATAA AATTGAGGAC ATGGCCATGA TGACCCACCT CCACGAACCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTATAC CTACTCGGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC GGAGGTGGTG 
TTGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCTCCAC ACATTTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGC GAGAATCAGT CGATCCTGAT CACCGGAGAA 
TCCGGTGCAG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GCTAGTGGAG ATAAGAAGAA GGAGGAGCAG CCAGCGGGCA AAATGCAGGG GACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ACTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAGAAGT CTAGGGTCAC TTTCCAGCTC 
AAGGCTGAAA GAAGCTACCA CATCTTTTAT CAGATCATGT CCAACAAGAA GCCAGAGCTA 
ATTGAGATGC TGCTGATCAC CACCAACCCA TACGACTATC ACTATGTGAG TCAAGGCGAG 
ATCACAGTGC CAAGCATTAA TGACCAGGAG GAGCTGATGG CCACCGATAG TGCCATTGAC 
ATCCTGGGCT TCACTCCTGA TGAAAAAACA GCCATTTACA AGCTGACGGG GGCTGTCATG 
CACTATGGCA ACCTGAAGTT CAAGCAGAAG CAGCGTGAAG AGCAGGCAGA GCCCGATGGC 
ACAGAAGTTG CTGACAAGGC TGCTTACCTG ATGGGTCTCA ACTCAGCAGA CCTGCTCAAG 
GCCCTGTGCT ACCCCCGAGT CAAAGTTGGG AATGAATTTG TGACCAAGGG TCAGACTGTG 
CAGCAGGTGT ACAACTCAGT TGGTGCCCTG GCAAAGGCTG TCTTTGAGAA GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGTCTGGA ACAGCTGTGC 
ATCAACTTCA CGAATGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT TGTGCTGGAA 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGGAGTTCA TTGACTTTGG GATGGACTTG 
GCTGCCTGCA TTGAGCTCAT TGAAAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA ACAACTTCCA GAAGCCTAAA CCTGGCAAAG GCAAGGCTGA GGCCCACTTC 
TCCCTGGTGC ACTATGCTGG CACAGTGGAC TACAACATCT CTGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGAATGAAAC TGTTGTGGGG CTGTACCAAA AATCATCCCT GAAGACACTG 
GCCTTACTGT TTGCTTCTGC TGGCGGAGAG GCAGAGAGTG GTGGTGGTGG CAAGAAGGGA 
GGCAAGAAGA AGGGTTCTTC TTTCCAGACT GTATCAGCTC TTTTCAGGGA GAACCTGAAC 
AAGCTGATGA CCAACCTACG GAGCACTCAC CCCCATTTTG TGCGCTGTAT CATCCCTAAT 
GAAACAAAAA CTCCCGGTGC CATGGAGCAT GAACTGGTGC TGCACCAGCT GCGTTGTAAT 
GGTGTGCTAG AAGGAATCAG AATTTGCAGA AAGGGATTCC CCAGCAGAAT CCTCTATGCA 
GACTTCAAAC AGAGATACAA GGTGCTTAAT GCCAGTGCCA TCCCCGAGGG ACAGTTCATT 
GACAGCAAGA AAGCTTCTGA GAAGCTACTT GGGTCAATCG ATGTGGACCA CACCCAGTAC 
AAATTTGGTC ACACCAAGGT GTTCTTCAAA GCTGGGCTGC TGGGACTTCT GGAGGAGATG 
AGGGATGAGA AGCTAGCACA GCTCATCACT CGCACACAGG CCAGGTGCAG GGGCTTCCTG 
ATGAGAGTGG AGTACCGCAG AATGGTGGAG AGGAGAGAAT CCATCTTCTG CATCCAGTAC 
AATATTCGTT CATTCATGAA TGTCAAGCAC TGGCCCTGGA TGAAGCTGTT CTTTAAGATC 
AAGCCGTTGC TGAAGAGCGC AGAGTCTGAG AAGGAAATGG CCAACATGAA AGGGGAGTTT 
GAGAAGACCA AGGAAGAACT TGCAAAGTCT GAGGCAAAGA GGAAAGAGTT GGAGGAGAAA 
ATGGTGTCCC TGCTGCAGGA GAAAAATGAC TTGCAGCTCC AAGTGCAGGC TGAAGCAGAT 
GCCTTAGCTG ATGCTGAGGA AAGGTGTGAC CAGCTCATCA AAACCAAAAT CCAGCTGGAA 
GCCAAAATTA AGGAGGTGAC AGAACGGGCT GAGGATGAGG AGGAAATTAA TGCTGAACTG 
ACGGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAGC TGAAGAAAGA CATAGATGAC 
CTGGAGTTAA CACTGGCCAA AGTTGAGAAG GAAAAGCATG CCACTGAAAA CAAGGTGAAA 
AACCTCACAG AGGAGATGGC AGCCTTGGAC GAGAACATTG CCAAGCTGAC AAAAGAGAAG 
AAAGCCCTCC AAGAGGCCCA TCAGCAGACA CTGGATGACC TGCAGGCTGA AGAGGACAAA 
GTCAATACGC TGACCAAAGC TAAAACAAAG CTGGAGCAGC AAGTGGACGA TCTGGAAGGG 
TCCCTGGAGC AAGAGAAGAA GCTGCGCATG GACCTCGAGA GAGCTAAGAG GAAACTTGAA 
GGAGACCTGA AGTTGGCCCA TGATAGCATA ATGGACTTGG AAAATGATAA ACAGCAGCTG 
GATGAGAAAC TGAAGAAGAA AGACTTTGAA ATCAGCCAGA TCCAGAGCAA AATTGAGGAT 
GAGCAAGCCC TGGGCATGCA GTTACAGAAG AAGATCAAGG AGCTGCAGGC CCGTACTGAG 
GAACTGGAGG AGGAAATCGA AGCAGAGCGA ACCTCTCGGG CAAAAGCAGA GAAGCACCGA 
GCTGACCTCT CCAGGGAGCT GGAGGAGATC AGTGAGCGCC TGGAGGAAGC AGGAGGGGCT 
ACAGCAGCTC AGATTGATAT GAACAAGAAG CGTGAGGCAG AATTTCAGAA GATGCGCCGT 
GACCTCGAAG AGGCCACGCT GCAGCACGAA GCCACGGCTG CTGCCCTGCG GAAGAAGCAT 
GCGGACAGCA CAGCGGAGCT TGGGGAGCAG ATCGACAACC TGCAACGAGT CAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GCTGAAGATG GAGATTGATG ACTTGGCCAG CAACATGGAG 
TCTGTCTCCA AAGCGAAGGC AAGTCTGGAG AAGACATGCC GAGCATTGGA AGATCAGCTG 
AGTGAGATTA AGACAAAAGA AGAGGAGCAT CAACGGATGA TCAATGATGT TAATGCTCAA 
AGAGCTCGTC TGCAGACAGA ATCAGGTGAA TATTCACGCC AGGTGGAGGA GAAAGATGCT 
CTGATTTCTC AGCTGTCTAG AGGCAAGCAG GCATTCACCC AACAGATTGA GGAACTCAAG 
AGGCACTTAG AGGAGGAGAT AAAGGCCAAG AATGCCCTAG CCCATGGTTT GCAGTCTGCT 
CGCCATGACT GTGATTTGCT CCGGGAACAA TATGAGGAGG AGCAGGAAGC CAAGGGTGAG 
CTGCAGCGTG CCCTGTCCAA GGCCAACAGT GAAGTGGCCC AGTGGAGAAC CAAATATGAG 
ACGGATGCTA TTCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCACAGCGC 
CTGCAAGATG CTGAGGAACA CGTCGAAGCC GTGAACTCCA AATGTGCTTC CCTGGAAAAG 
ACAAAGCAGA GGCTGCAGAA TGAGGTGGAG GACCTGATGA TTGACGTGGA GCGAGCAAAT 
TCTGCCTGCG CAGCTCTGGA CAAGAAGCAG AAGAACTTTG ACAAGATCCT GTCGGAGTGG 
AAGCAGAAGT ACGAGGAAAC GCAGGCTGAG CTGGAGGCCT CCCAGAAGGA GTCTCGCTCC 
CTCAGCACAG AGCTGTTTAA GATGAAGAAT GCCTATGAGG AATCCCTGGA CCACCTGGAA 
ACACTGAAGC GTGAGAACAA GAACTTGCAG CAGGAGATTT CTGACCTCAC GGAGCAGATT 
GCAGAGGGAG GAAAGGCTAT TCATGAGCTG GAGAAAGTCA AGAAGCAGAT TGAGCAGGAG 
AAATCTGAAC TTCAAGCCTC CCTAGAGGAA GCAGAAGCCT CCCTGGAACA TGAAGAAGGG 
AAGATCCTGC GCCTTCAACT GGAGCTCAAC CAGGTCAAGT CTGAGATTGA CAGGAAGATA 
GCAGAGAAAG ATGAGGAAAT TGACCAGCTG AAGAGAAACC ATCTCAGAAT TGTGGAGTCC 
ATGCAGAGCA CTCTGGATGC TGAGGTCAGG AGCAGGAATG AGGCCCTGAG GCTGAAGAAG 
AAGATGGAGG GAGACCTGAA TGAAATGGAG ATCCAGCTCA ACCATGCCAA CCGCATGGCT 
GCAGAGGCAC AAAGGAACCT GAGAAACACA CAAGGAGTGC TCAAGGATAC CCAGATACAC 
TTGGACGATG CTCTCAGGTC ACAGGAAGAC CTGAAGGAGC AGGTGGCCAT GGTGGAGCGC 
AGAGCAAACC TGTTGCAGGC TGAAACTGAA GAGCTACGGG CAGCGCTGGA GCAGACAGAG 
CGCTCGAGGA AAGTGGCTGA GCAGGAGCTT CTGGATGCAA GTGAACGTGT GCAGCTCCTC 
CATACCCAGA ACACCAGCTT GATCAACACC AAGAAGAAGC TAGAATCAGA CATTTCCCAA 
ATTCAGAGTG AAATGGAGGA TACCATCCAG GAAGCCCGCA ATGCTGAGGA GAAGGCCAAG 
AAGGCCATCA CCGATGCAGC CATGATGGCA GAAGAGCTGA AGAAGGAGCA GGACACCAGT 
GCCCACCTGG AGAGGATGAA GAAGAACCTG GACCAGACGG TGAAGGACCT GCAGCACCGC 
CTGGATGAGG CAGAGCAGCT GGCCCTGAAG GGAGGCAAGA AGCAAATCCA GAAGCTGGAG 
GCCAGGGTGC GGGAGCTGGA AGGGGAGGTT GATGCTGAGC AGAAGCGCAG CGCTGAAGCC 
GTGAAGGGTG TACGCAAGTA CGAGAGGAGG GTGAAGGAGC TGACCTACCA GTCTGAAGAA 
GACCGGAAGA ATGTTCTCAG GCTCCAGGAC CTGGTGGACA AGCTGCAGAT GAAGGTGAAA 
TCCTACAAGA GGCAAGCTGA GGAGGCTGAG GAGCTGTCCA ATGTCAACCT TTCCAAATTC 
CGCAAGATCC AGCACGAGCT GGAGGAAGCC GAGGAGCGGG CTGACATTGC AGAGTCACAG 
GTCAACAAGC TCCGAGTGAA GAGTCGTGAG TTTCATAAGA AGATAGAAGA GGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001231409.1
CDS	85..5904
Translation	MSSDAEMAIFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVVHAKESYVKSTIQSKESGKVTVKTESGETLTVKEDQIFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGDKKKEEQPAGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDYHYVSQGEITVPSINDQEELMATDSAIDILGFTPDEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADLLKALCYPRVKVGNEFVTKGQTVQQVYNSVGALAKAVFEKMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKPGKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNETVVGLYQKSSLKTLALLFASAGGEAESGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLMRVEYRRMVERRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKGEFEKTKEELAKSEAKRKELEEKMVSLLQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDENIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQALGMQLQKKIKELQARTEELEEEIEAERTSRAKAEKHRADLSRELEEISERLEEAGGATAAQIDMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKASLEKTCRALEDQLSEIKTKEEEHQRMINDVNAQRARLQTESGEYSRQVEEKDALISQLSRGKQAFTQQIEELKRHLEEEIKAKNALAHGLQSARHDCDLLREQYEEEQEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERANSACAALDKKQKNFDKILSEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSELQASLEEAEASLEHEEGKILRLQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRIVESMQSTLDAEVRSRNEALRLKKKMEGDLNEMEIQLNHANRMAAEAQRNLRNTQGVLKDTQIHLDDALRSQEDLKEQVAMVERRANLLQAETEELRAALEQTERSRKVAEQELLDASERVQLLHTQNTSLINTKKKLESDISQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQMKVKSYKRQAEEAEELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRVKSREFHKKIEEEE

Target ORF information:

RefSeq Version	XM_001231408.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin, heavy chain 1F, skeletal muscle (MYH1F), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001231408.4

ORF Insert Sequence:

ATGTCATCAG ATGCTGAGAT GGCCATATTT GGGGAGGCAG CCCCTTACCT CCGAAAGTCA 
GAAAAGGAGA GGATTGAGGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTACATGCTA AAGAATCCTA TGTGAAAAGT ACAATCCAGA GTAAAGAATC GGGGAAGGTC 
ACTGTCAAGA CTGAAAGTGG AGAAACTCTA ACCGTGAAGG AAGATCAAAT CTTCTCCATG 
AACCCTCCCA AGTATGATAA AATTGAGGAC ATGGCCATGA TGACCCACCT CCACGAACCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTATAC CTACTCGGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC GGAGGTGGTG 
TTGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCTCCAC ACATTTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGC GAGAATCAGT CGATCCTGAT CACCGGAGAA 
TCCGGTGCAG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GCTAGTGGAG ATAAGAAGAA GGAGGAGCAG CCAGCGGGCA AAATGCAGGG GACACTTGAG 
GATCAAATCA TCAGTGCCAA CCCCCTGCTG GAGGCCTTTG GAAATGCCAA GACTGTGAGG 
AATGACAACT CCTCACGCTT TGGTAAATTC ATCAGAATCC ACTTTGGGGC CACAGGCAAA 
CTGGCTTCTG CTGACATTGA AACATATCTG CTGGAGAAGT CTAGGGTCAC TTTCCAGCTC 
AAGGCTGAAA GAAGCTACCA CATCTTTTAT CAGATCATGT CCAACAAGAA GCCAGAGCTA 
ATTGAGATGC TGCTGATCAC CACCAACCCA TACGACTATC ACTATGTGAG TCAAGGCGAG 
ATCACAGTGC CAAGCATTAA TGACCAGGAG GAGCTGATGG CCACCGATAG TGCCATTGAC 
ATCCTGGGCT TCACTCCTGA TGAAAAAACA GCCATTTACA AGCTGACGGG GGCTGTCATG 
CACTATGGCA ACCTGAAGTT CAAGCAGAAG CAGCGTGAAG AGCAGGCAGA GCCCGATGGC 
ACAGAAGTTG CTGACAAGGC TGCTTACCTG ATGGGTCTCA ACTCAGCAGA CCTGCTCAAG 
GCCCTGTGCT ACCCCCGAGT CAAAGTTGGG AATGAATTTG TGACCAAGGG TCAGACTGTG 
CAGCAGGTGT ACAACTCAGT TGGTGCCCTG GCAAAGGCTG TCTTTGAGAA GATGTTCTTG 
TGGATGGTTG TTCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGTGTCCTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGTCTGGA ACAGCTGTGC 
ATCAACTTCA CGAATGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT TGTGCTGGAA 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGGAGTTCA TTGACTTTGG GATGGACTTG 
GCTGCCTGCA TTGAGCTCAT TGAAAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC TGACACCTCT TTCAAGAACA AGCTCTATGA CCAGCATCTG 
GGCAAGTCCA ACAACTTCCA GAAGCCTAAA CCTGGCAAAG GCAAGGCTGA GGCCCACTTC 
TCCCTGGTGC ACTATGCTGG CACAGTGGAC TACAACATCT CTGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGAATGAAAC TGTTGTGGGG CTGTACCAAA AATCATCCCT GAAGACACTG 
GCCTTACTGT TTGCTTCTGC TGGCGGAGAG GCAGAGAGTG GTGGTGGTGG CAAGAAGGGA 
GGCAAGAAGA AGGGTTCTTC TTTCCAGACT GTATCAGCTC TTTTCAGGGA GAACCTGAAC 
AAGCTGATGA CCAACCTACG GAGCACTCAC CCCCATTTTG TGCGCTGTAT CATCCCTAAT 
GAAACAAAAA CTCCCGGTGC CATGGAGCAT GAACTGGTGC TGCACCAGCT GCGTTGTAAT 
GGTGTGCTAG AAGGAATCAG AATTTGCAGA AAGGGATTCC CCAGCAGAAT CCTCTATGCA 
GACTTCAAAC AGAGATACAA GGTGCTTAAT GCCAGTGCCA TCCCCGAGGG ACAGTTCATT 
GACAGCAAGA AAGCTTCTGA GAAGCTACTT GGGTCAATCG ATGTGGACCA CACCCAGTAC 
AAATTTGGTC ACACCAAGGT GTTCTTCAAA GCTGGGCTGC TGGGACTTCT GGAGGAGATG 
AGGGATGAGA AGCTAGCACA GCTCATCACT CGCACACAGG CCAGGTGCAG GGGCTTCCTG 
ATGAGAGTGG AGTACCGCAG AATGGTGGAG AGGAGAGAAT CCATCTTCTG CATCCAGTAC 
AATATTCGTT CATTCATGAA TGTCAAGCAC TGGCCCTGGA TGAAGCTGTT CTTTAAGATC 
AAGCCGTTGC TGAAGAGCGC AGAGTCTGAG AAGGAAATGG CCAACATGAA AGGGGAGTTT 
GAGAAGACCA AGGAAGAACT TGCAAAGTCT GAGGCAAAGA GGAAAGAGTT GGAGGAGAAA 
ATGGTGTCCC TGCTGCAGGA GAAAAATGAC TTGCAGCTCC AAGTGCAGGC TGAAGCAGAT 
GCCTTAGCTG ATGCTGAGGA AAGGTGTGAC CAGCTCATCA AAACCAAAAT CCAGCTGGAA 
GCCAAAATTA AGGAGGTGAC AGAACGGGCT GAGGATGAGG AGGAAATTAA TGCTGAACTG 
ACGGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAGC TGAAGAAAGA CATAGATGAC 
CTGGAGTTAA CACTGGCCAA AGTTGAGAAG GAAAAGCATG CCACTGAAAA CAAGGTGAAA 
AACCTCACAG AGGAGATGGC AGCCTTGGAC GAGAACATTG CCAAGCTGAC AAAAGAGAAG 
AAAGCCCTCC AAGAGGCCCA TCAGCAGACA CTGGATGACC TGCAGGCTGA AGAGGACAAA 
GTCAATACGC TGACCAAAGC TAAAACAAAG CTGGAGCAGC AAGTGGACGA TCTGGAAGGG 
TCCCTGGAGC AAGAGAAGAA GCTGCGCATG GACCTCGAGA GAGCTAAGAG GAAACTTGAA 
GGAGACCTGA AGTTGGCCCA TGATAGCATA ATGGACTTGG AAAATGATAA ACAGCAGCTG 
GATGAGAAAC TGAAGAAGAA AGACTTTGAA ATCAGCCAGA TCCAGAGCAA AATTGAGGAT 
GAGCAAGCCC TGGGCATGCA GTTACAGAAG AAGATCAAGG AGCTGCAGGC CCGTACTGAG 
GAACTGGAGG AGGAAATCGA AGCAGAGCGA ACCTCTCGGG CAAAAGCAGA GAAGCACCGA 
GCTGACCTCT CCAGGGAGCT GGAGGAGATC AGTGAGCGCC TGGAGGAAGC AGGAGGGGCT 
ACAGCAGCTC AGATTGATAT GAACAAGAAG CGTGAGGCAG AATTTCAGAA GATGCGCCGT 
GACCTCGAAG AGGCCACGCT GCAGCACGAA GCCACGGCTG CTGCCCTGCG GAAGAAGCAT 
GCGGACAGCA CAGCGGAGCT TGGGGAGCAG ATCGACAACC TGCAACGAGT CAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GCTGAAGATG GAGATTGATG ACTTGGCCAG CAACATGGAG 
TCTGTCTCCA AAGCGAAGGC AAGTCTGGAG AAGACATGCC GAGCATTGGA AGATCAGCTG 
AGTGAGATTA AGACAAAAGA AGAGGAGCAT CAACGGATGA TCAATGATGT TAATGCTCAA 
AGAGCTCGTC TGCAGACAGA ATCAGGTGAA TATTCACGCC AGGTGGAGGA GAAAGATGCT 
CTGATTTCTC AGCTGTCTAG AGGCAAGCAG GCATTCACCC AACAGATTGA GGAACTCAAG 
AGGCACTTAG AGGAGGAGAT AAAGGCCAAG AATGCCCTAG CCCATGGTTT GCAGTCTGCT 
CGCCATGACT GTGATTTGCT CCGGGAACAA TATGAGGAGG AGCAGGAAGC CAAGGGTGAG 
CTGCAGCGTG CCCTGTCCAA GGCCAACAGT GAAGTGGCCC AGTGGAGAAC CAAATATGAG 
ACGGATGCTA TTCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCACAGCGC 
CTGCAAGATG CTGAGGAACA CGTCGAAGCC GTGAACTCCA AATGTGCTTC CCTGGAAAAG 
ACAAAGCAGA GGCTGCAGAA TGAGGTGGAG GACCTGATGA TTGACGTGGA GCGAGCAAAT 
TCTGCCTGCG CAGCTCTGGA CAAGAAGCAG AAGAACTTTG ACAAGATCCT GTCGGAGTGG 
AAGCAGAAGT ACGAGGAAAC GCAGGCTGAG CTGGAGGCCT CCCAGAAGGA GTCTCGCTCC 
CTCAGCACAG AGCTGTTTAA GATGAAGAAT GCCTATGAGG AATCCCTGGA CCACCTGGAA 
ACACTGAAGC GTGAGAACAA GAACTTGCAG CAGGAGATTT CTGACCTCAC GGAGCAGATT 
GCAGAGGGAG GAAAGGCTAT TCATGAGCTG GAGAAAGTCA AGAAGCAGAT TGAGCAGGAG 
AAATCTGAAC TTCAAGCCTC CCTAGAGGAA GCAGAAGCCT CCCTGGAACA TGAAGAAGGG 
AAGATCCTGC GCCTTCAACT GGAGCTCAAC CAGGTCAAGT CTGAGATTGA CAGGAAGATA 
GCAGAGAAAG ATGAGGAAAT TGACCAGCTG AAGAGAAACC ATCTCAGAAT TGTGGAGTCC 
ATGCAGAGCA CTCTGGATGC TGAGGTCAGG AGCAGGAATG AGGCCCTGAG GCTGAAGAAG 
AAGATGGAGG GAGACCTGAA TGAAATGGAG ATCCAGCTCA ACCATGCCAA CCGCATGGCT 
GCAGAGGCAC AAAGGAACCT GAGAAACACA CAAGGAGTGC TCAAGGATAC CCAGATACAC 
TTGGACGATG CTCTCAGGTC ACAGGAAGAC CTGAAGGAGC AGGTGGCCAT GGTGGAGCGC 
AGAGCAAACC TGTTGCAGGC TGAAACTGAA GAGCTACGGG CAGCGCTGGA GCAGACAGAG 
CGCTCGAGGA AAGTGGCTGA GCAGGAGCTT CTGGATGCAA GTGAACGTGT GCAGCTCCTC 
CATACCCAGA ACACCAGCTT GATCAACACC AAGAAGAAGC TAGAATCAGA CATTTCCCAA 
ATTCAGAGTG AAATGGAGGA TACCATCCAG GAAGCCCGCA ATGCTGAGGA GAAGGCCAAG 
AAGGCCATCA CCGATGCAGC CATGATGGCA GAAGAGCTGA AGAAGGAGCA GGACACCAGT 
GCCCACCTGG AGAGGATGAA GAAGAACCTG GACCAGACGG TGAAGGACCT GCAGCACCGC 
CTGGATGAGG CAGAGCAGCT GGCCCTGAAG GGAGGCAAGA AGCAAATCCA GAAGCTGGAG 
GCCAGGGTGC GGGAGCTGGA AGGGGAGGTT GATGCTGAGC AGAAGCGCAG CGCTGAAGCC 
GTGAAGGGTG TACGCAAGTA CGAGAGGAGG GTGAAGGAGC TGACCTACCA GTCTGAAGAA 
GACCGGAAGA ATGTTCTCAG GCTCCAGGAC CTGGTGGACA AGCTGCAGAT GAAGGTGAAA 
TCCTACAAGA GGCAAGCTGA GGAGGCTGAG GAGCTGTCCA ATGTCAACCT TTCCAAATTC 
CGCAAGATCC AGCACGAGCT GGAGGAAGCC GAGGAGCGGG CTGACATTGC AGAGTCACAG 
GTCAACAAGC TCCGAGTGAA GAGTCGTGAG TTTCATAAGA AGATAGAAGA GGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence for the expression of neonatal skeletal myosin heavy chain in primary myocardium and cardiac conduction tissue in the developing chick heart.
Developmental dynamics : an official publication of the American Association of Anatomists217(1)37-49(2000 Jan)
Machida S,Matsuoka R,Noda S,Hiratsuka E,Takagaki Y,Oana S,Furutani Y,Nakajima H,Takao A,Momma K

Analysis of the chicken fast myosin heavy chain family. Localization of isoform-specific antibody epitopes and regions of divergence.
Journal of molecular biology225(4)1143-51(1992 Jun)
Moore LA,Arrizubieta MJ,Tidyman WE,Herman LA,Bandman E

A canonical sequence organization at the 5'-end of the myosin heavy chain genes.
The Journal of biological chemistry261(14)6613-8(1986 May)
Kropp K,Gulick J,Robbins J

The chicken myosin heavy chain family.
The Journal of biological chemistry261(14)6606-12(1986 May)
Robbins J,Horan T,Gulick J,Kropp K

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MYH1F cDNA ORF clone, Gallus gallus(chicken)

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