FBN1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	FBN1
Entrez Gene ID	281154
Full Name	fibrillin 1
General protein information	Preferred Names fibrillin 1 Names fibrillin-1 MP340
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA Protein Name

XM_015473179.1 XP_015328665.1 fibrillin-1 isoform X1

XM_015473179.2 XP_015328665.1 fibrillin-1 isoform X1

NM_174053.2 NP_776478.1 fibrillin-1 precursor
Pathways from BioSystems

mRNA	Protein	Name
XM_015473179.1	XP_015328665.1	fibrillin-1 isoform X1
XM_015473179.2	XP_015328665.1	fibrillin-1 isoform X1
NM_174053.2	NP_776478.1	fibrillin-1 precursor

Homologies

Homo sapiens (human)	FBN1	NP_000129.3
	FBN1	XP_001113107.2
Bos taurus (cattle)	FBN1	NP_776478.1
Canis lupus familiaris (dog)	FBN1	XP_861706.2
Mus musculus (house mouse)	Fbn1	NP_032019.2
Gallus gallus (chicken)	FBN1	XP_413815.4
Xenopus tropicalis (tropical clawed frog)	fbn1	XP_002936615.2
Pan troglodytes (chimpanzee)	FBN1	XP_001149266.3
Rattus norvegicus (Norway rat)	Fbn1	NP_114013.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of an FBN1 mutation in bovine Marfan syndrome-like disease.
Hirano T, Matsuhashi T, Kobayashi N, Watanabe T, Sugimoto Y
Animal genetics43(1)11-7(2012 Feb)

Microfibrils and fibrillin-1 induce integrin-mediated signaling, proliferation and migration in human endothelial cells.
Mariko B, Ghandour Z, Raveaud S, Quentin M, Usson Y, Verdetti J, Huber P, Kielty C, Faury G
American journal of physiology. Cell physiology299(5)C977-87(2010 Nov)

Fibrillins and latent TGFbeta binding proteins in bovine ovaries of offspring following high or low protein diets during pregnancy of dams.
Prodoehl MJ, Irving-Rodgers HF, Bonner WM, Sullivan TM, Micke GC, Gibson MA, Perry VE, Rodgers RJ
Molecular and cellular endocrinology307(1-2)133-41(2009 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Bovine model of Marfan syndrome results from an amino acid change (c.3598G > A, p.E1200K) in a calcium-binding epidermal growth factor-like domain of fibrillin-1.
Singleton AC, Mitchell AL, Byers PH, Potter KA, Pace JM
Human mutation25(4)348-52(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study concludes that the c.8227-1G>A mutation is causative for Marfan syndrome-like disease.
Title: Identification of an FBN1 mutation in bovine Marfan syndrome-like disease.

Data suggest that genetic fibrillin-1 deficiency could alter normal endothelial signaling.
Title: Microfibrils and fibrillin-1 induce integrin-mediated signaling, proliferation and migration in human endothelial cells.

This is the first study to investigate the expression and localization of fibrillin proteins and latent TGF-beta binding proteins affecting TGFbeta bioavailability in the ovary.
Title: Fibrillins and latent TGFbeta binding proteins in bovine ovaries of offspring following high or low protein diets during pregnancy of dams.

a calcium-binding epidermal growth factor-like domain of fibrillin-1 c.3598G > A, p.E1200K mutation is responsible for a bovine model of Marfan syndrome
Title: Bovine model of Marfan syndrome results from an amino acid change (c.3598G > A, p.E1200K) in a calcium-binding epidermal growth factor-like domain of fibrillin-1.

The coordinate upregulation of fibrillin-1 and fibrillin-2 expression with the onset of tropoelastin production is consistent with a role in elastic fiber assembly.
Title: Fibrillin-1 and fibrillin-2 show temporal and tissue-specific regulation of expression in developing elastic tissues.

The following FBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44778	NM_174053.2 Latest version!	Bos taurus fibrillin 1 (FBN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa91701	XM_015473179.2 Latest version!	Bos taurus fibrillin 1 (FBN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OBa91701	XM_015473179.1	Bos taurus fibrillin 1 (FBN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FBN1 ( NM_174053.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776478.1 Bos taurus fibrillin 1 (FBN1), mRNA.

Price & Availability↑

CloneID	OBa44778
Clone ID Related Accession (Same CDS sequence)	NM_174053.2
Accession Version	NM_174053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	fibrillin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L28748.1. On Aug 30, 2012 this sequence version replaced NM_174053.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L28748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGCGGCGAG GGGGGCTGCT GGAGGTCGCC TTGGGATTTA CCGTGCTCTT AGCGTCCTAC 
ACGAGCCATG GGGCGGATAC CAATTTGGAG GCTGGGAACG TGAAGGAAAC CAGAGCCAAC 
CGGGCCAAGA GAAGAGGTGG CGGAGGACAC AACGCGCTTA AAGGACCCAA TGTCTGTGGA 
TCACGTTATA ATGCTTACTG TTGCCCTGGA TGGAAGACTT TACCTGGTGG AAATCAGTGT 
ATTGTCCCCA TTTGCCGGCA TTCCTGTGGG GATGGATTTT GTTCGAGGCC AAATATGTGC 
ACTTGCCCAT CTGGTCAGAT AGCTCCTTCC TGTGGCTCCA GATCCATCCA ACATTGTAAC 
ATTCGCTGCA TGAATGGAGG TAGCTGCAGT GATGATCATT GTCTGTGCCA GAAAGGATAC 
ATAGGGACTC ACTGTGGACA ACCCGTCTGT GAAAGTGGCT GTCTTAACGG AGGCAGGTGC 
GTGGCCCCGA ATCGATGTGC GTGCACTTAC GGCTTTACTG GACCCCAGTG TGAAAGAGAT 
TACAGGACAG GTCCATGTTT TACTGTGATC AGCAACCAGA TGTGCCAGGG ACAGCTCAGC 
GGGATTGTCT GCACGAAAAC GCTCTGCTGT GCCACGGTCG GCCGTGCCTG GGGCCACCCC 
TGTGAGATGT GTCCCGCCCA GCCCCACCCC TGTCGCCGCG GCTTCATTCC AAATATCCGC 
ACAGGAGCTT GTCAAGATGT GGACGAATGC CAGGCCATCC CTGGGCTCTG TCAAGGAGGA 
AATTGCATTA ATACTGTTGG GTCTTTTGAG TGCAAATGCC CTGCTGGACA CAAATTTAAT 
GAAGTGTCAC AAAAATGTGA AGATATTGAC GAATGTAGCA CTATTCCTGG AATCTGTGAT 
GGGGGCGAAT GTACGAACAC AGTCAGCAGT TACTTTTGCA AATGTCCCCC TGGTTTTTAC 
ACCTCTCCAG ACGGTACCAG ATGCATAGAT GTCCGCCCAG GATACTGTTA CACGGCTCTG 
GCAAATGGGC GCTGTTCTAA CCAGCTGCCA CAGTCCATCA CCAAGATGCA GTGCTGCTGT 
GATGCCGGCC GGTGCTGGTC TCCGGGGGTC ACGGTCGCCC CCGAGATGTG TCCCATCAGA 
GCAACTGAGG ATTTCAACAA GCTGTGCTCC GTCCCTATGG TAATTCCCGA GAGGCCAGGA 
TATCCTCCTC CACCCCTTGG CCCCGTTCCT CCCGTTCAGC CTGTCCCTCC TGGCTTTCCT 
CCTGGGCCTC AAATCATGAT CCCTCGACCA CCAGTGGAAT ATCCGTATCC ATCGCGGGAA 
CCACCAAGGG TGCTGCCAGT CAACGTCACT GATTACTGCC AGCTCTTTCG CTATCTCTGT 
CAAAATGGAC GCTGCATCCC AACCCCCGGG AGCTACCGGT GTGAGTGCAA CAAAGGATTC 
CAGCTGGACC TCCGTGGGGA GTGCATTGAT GTTGATGAAT GTGAGAAGAA CCCTTGTGCT 
GGTGGCGAGT GTATTAACAC GCAGGGCTCT TACACCTGTC AGTGCCGACC TGGTTATCAG 
AGCACACTCA CGCGGACAGA GTGCCGAGAC ATTGATGAAT GTTTACAGAA TGGCCGTATC 
TGTAATAATG GACGTTGTAT CAACACGGAT GGCAGTTTCC ATTGTGTGTG TAATGCAGGA 
TTTCACGTCA CACGAGATGG GAAGAACTGT GAAGATATGG ATGAATGCAG CATAAGGAAT 
ATGTGCCTTA ATGGAATGTG TATCAACGAA GATGGCAGTT TCAAATGTAT TTGCAAACCT 
GGATTTCAGC TGGCATCAGA TGGGCGTTAT TGTAAAGATA TTAATGAGTG TGAAACCCTT 
GGGATCTGCA TGAACGGGCG TTGCGTCAAC ACTGATGGCT CCTACAGATG TGAATGCTTT 
CCTGGACTGG CCGTGGGTCT GGACGGACGT GTGTGTGTTG ACACACACAT GCGGAGCACA 
TGCTATGGTG GATACAAAAG AGGCCAGTGT GTCAAACCCT TGTTTGGTGC TGTTACCAAG 
TCTGAATGCT GTTGTGCCAG CACTGAGTAT GCTTTCGGGG AACCTTGCCA ACCATGTCCT 
TCACAGAATT CAGCGGAGTA TCAGGCGCTC TGCAGCAGTG GGCCAGGAAT AACGTCAGCA 
GGCAGCGATA TAAATGAATG TGCATTAGAT CCTGATATTT GCCCAAATGG AATTTGTGAA 
AATCTTCGTG GGACCTACAA ATGTATATGC AATTCAGGAT ATGAAGTGGA TTCAACTGGG 
AAAAACTGTG TTGATATTAA TGAATGTGTA TTGAATAGTC TGCTTTGTGA CAATGGACAA 
TGTAGAAATA CTCCTGGAAG TTTTGTCTGT ACCTGCCCCA AGGGATTTAT ATACAAACCT 
GAACTGAAAA CCTGTGAAGA CATCGATGAA TGTGAATCAA GTCCTTGCAT TAATGGAGTC 
TGCAAGAACA GTCCGGGCTC TTTCATTTGT GAATGTTCTT CTGAAAGTAC TTTGGATCCA 
ACAAAAACCA TCTGCATAGA AACCATTAAG GGCACTTGTT GGCAGACTGT CATCGATGGG 
CGGTGTGAGA TCAACATCAA TGGAGCCACA TTGAAGTCCC AGTGCTGTTC ATCCCTTGGT 
GCTGCCTGGG GGAGCCCATG CACCCCATGC CAAGTCGATC CCATATGTGG GAAAGGCTAT 
TCAAGAATTA AAGGAACACA GTGTGAAGAT ATAGATGAAT GTGAAGTGTT CCCAGGAGTC 
TGCAAGAATG GCTTGTGTGT TAACTCCAAG GGATCATTTA AATGTCAGTG TCCCAGTGGG 
ATGACTTTGG ATGCCACAGG GAGGATTTGC CTAGATATCC GCCTGGAAAC CTGCTTCCTG 
AGGTACGAGG ACGAGGAGTG CACCCTGCCC GTGGCTGGGC GCCACCGCAT GGACGCCTGC 
TGCTGCTCAG TCGGGGCAGC CTGGGGCACC GAGGAGTGTG AGGAGTGTCC CGTGAGAAAC 
ACACCTGAGT ACGAGGAGCT CTGTCCCAGA GGACCCGGAT TCGCCACAAA AGAAATTACA 
AATGGCAAAC GTTTTTTCAA AGACATCAAC GAGTGTAAGA TGATTCCCAA CCTGTGCACC 
CACGGCAAGT GCAGGAACAC CATTGGCAGC TTCAAGTGCA GGTGTGACAG CGGCTTTGCT 
CTGGATTCTG AAGAAAGGAA CTGCACAGAC ATCGACGAAT GCCGCATATC CCCTGACCTC 
TGTGGCCGAG GCCAGTGTGT GAACACCCCC GGGGACTTTG AGTGCAAGTG CGATGAAGGC 
TATGAAAGCG GATTCATGAT GATGAAGAAC TGCATGGATA TCGATGAGTG TCAGAGAGAT 
CCGCTGTTGT GCCGAGGAGG CGTGTGCCTG AACACTGAGG GAAGTTACCG CTGTGAATGT 
CCCCCTGGCC ATCAGCTGGC CCCCAACATC TCGGCGTGTA TAGACATCAA CGAGTGTGAA 
CTAAGTGCAC ACCTCTGCCC CCATGGCCGC TGCGTGAACC TCATAGGAAA GTACCAGTGT 
GCCTGCAACC CTGGCTACCA CTCTACCCCT GACAGGCTGT TTTGTGTTGA CATTGATGAA 
TGCAGCATAA TGAACGGTGG CTGTGAGACC TTCTGCACAA ACTCTGAAGG TAGCTACGAA 
TGTAGCTGTC AGCCAGGATT TGCACTGATG CCTGACCAGA GGTCCTGCAC AGACATTGAT 
GAGTGTGAAG ATAACCCCAA TATCTGTGAT GGTGGTCAGT GTACAAACAT ACCTGGAGAG 
TACAGGTGCT TGTGTTATGA TGGATTCATG GCATCTGAAG ACATGAAGAC TTGCGTAGAT 
GTCAATGAAT GTGACCTGAA TCCAAATATC TGCCTAAGTG GAACCTGTGA AAACACCAAA 
GGCTCATTTA TCTGCCACTG TGATATGGGC TATTCAGGCA AAAAAGGAAA AACAGGCTGT 
ACAGACATCA ATGAATGTGA AATTGGAGCA CACAACTGCG ACAGACATGC TGTATGTACC 
AACACAGCAG GAAGCTTCAA ATGCAGCTGT AGCCCCGGGT GGATTGGAGA TGGCATTAAG 
TGCACTGACC TGGACGAATG CTCCAATGGA ACCCACATGT GCAGCCAACA TGCAGACTGC 
AAGAACACCA TGGGCTCCTA CCGCTGCCTC TGCAAAGAGG GGTACACGGG CGATGGCTTC 
ACTTGTACAG ATCTTGATGA GTGCTCTGAG AATCTAAACC TGTGTGGCAA CGGCCAGTGC 
CTTAACGCCC CAGGAGGATA CCGCTGTGAA TGTGACATGG GCTTTGTGCC CAGTGCTGAT 
GGGAAAGCCT GTGAAGATAT CGATGAGTGC TCTCTTCCGA ACATCTGTGT TTTTGGAACT 
TGCCACAACC TCCCTGGCCT CTTCCGCTGT GAATGTGAGA TCGGCTATGA ACTGGACAGA 
AGTGGTGGGA ACTGCACAGA CGTTAATGAA TGCCTGGATC CAACCACGTG CATCAGTGGG 
AACTGTGTCA ATACTCCAGG CAGCTATACC TGTGACTGTC CACCCGATTT TGAGCTGAAT 
CCAACTCGAG TTGGCTGTGT CGATACCCGC TCTGGAAACT GCTATTTGGA TATCCGTCCT 
CGGGGAGACA ATGGAGATAC AGCCTGCAGC AATGAAATTG GGGTTGGAGT GTCCAAGGCT 
TCTTGCTGCT GCTCTCTGGG TAAAGCCTGG GGCACTCCTT GTGAGCTGTG CCCGCCTGTG 
AACACATCTG AGTACAAAAT TCTTTGTCCT GGAGGAGAAG GTTTTCGACC CAACCCTATC 
ACCGTTATAT TGGAAGATAT TGATGAGTGC CAGGAGCTTC CAGGGTTGTG CCAAGGCGGG 
AAATGTATCA ATACCTTCGG CAGCTTCCAG TGCCGCTGTC CAACGGGCTA CTACCTGAAT 
GAAGATACCC GAGTGTGTGA TGATGTGAAT GAATGTGAAA CTCCCGGAAT CTGTGGTCCA 
GGGACATGTT ACAACACTGT TGGCAACTAC ACCTGTATCT GCCCTCCAGA CTACATGCAA 
GTGAATGGGG GGAATAATTG CATGGATATG AGGAGGAGTC TGTGCTACAG GAACTATTAT 
GCTGACAACC AGACCTGCGA TGGAGAACTG TTGTTCAACA TGACCAAGAA GATGTGCTGT 
TGTTCCTACA ACATTGGCCG GGCCTGGAAC AAGCCCTGTG AACAGTGCCC CATTCCAAGC 
ACAGATGAAT TTGCTACACT CTGTGGAAGT CAACGGCCAG GCTTTGTCAT CGACATCTAT 
ACCGGTTTAC CTGTCGATAT CGACGAGTGT CGGGAGATCC CAGGGGTCTG TGAAAACGGT 
GTGTGTATCA ACATGGTCGG CAGCTTCCGT TGTGAATGTC CAGTGGGCTT CTTCTATAAT 
GACAAGTTGC TGGTTTGTGA AGACATTGAC GAGTGTCAGA ACGGCCCAGT GTGCCAGCGA 
AACGCAGAGT GCATCAACAC CGCGGGCAGC TACCGCTGTG ATTGCAAACC CGGCTACCGA 
TTCACCTCCA CGGGGCAGTG CAATGATCGC AATGAATGTC AAGAAATCCC CAATATATGC 
AGCCATGGAC AGTGTATTGA CACAGTTGGA AGCTTTTATT GCCTTTGCCA CACTGGTTTT 
AAAACAAACG CTGATCAAAC CATGTGTTTG GACATAAATG AGTGTGAAAG AGATGCCTGT 
GGGAATGGCA CTTGCCGGAA CACGATTGGT TCCTTCAACT GCCGCTGCAA TCACGGTTTC 
ATCCTTTCCC ACAACAATGA CTGCATAGAT GTTGATGAAT GTGCAACTGG AAATGGGAAC 
CTTTGCAGAA ATGGCCAGTG TATCAATACA GTGGGGTCCT TCCAGTGTCA GTGCAATGAA 
GGCTATGAGG TGGCTCCAGA TGGGAGAACC TGTGTGGATA TCAATGAATG TCTCCTAGAT 
CCCAGAAAAT GTGCCCCAGG TACCTGTCAA AACTTGGATG GGTCCTACAG ATGTATCTGT 
CCACCTGGAT ACAGTCTCCA GAATGACAAG TGTGAAGATA TTGATGAATG TGTTGAAGAG 
CCAGAAATCT GTGCCTTGGG CACATGCAGT AACACTGAAG GCAGCTTCAA ATGTCTGTGT 
CCAGACGGGT TCTCCTTGTC CTCCACTGGA AGAAGGTGCC AAGATTTACG AATGAGCTAC 
TGTTACGCGA AGTTTGAAGG AGGAAAGTGT TCATCACCCA AATCCAGAAA TCACTCCAAA 
CAGGAATGCT GCTGTGCCTT GAAGGGAGAA GGTTGGGGAG ATCCCTGCGA GCTGTGCCCC 
ACTGAACCCG ATGAGGCCTT CCGCCAGATC TGTCCCTATG GAAGTGGCAT CATCGTGGGA 
CCTGATGATT CAGCAGTTGA CATGGATGAG TGCAAAGAAC CCGATGTCTG TAAACACGGG 
CAGTGCATCA ACACCGATGG GTCCTATCGC TGCGAATGTC CCTTTGGCTA CATTCTGCAA 
GGGAATGAAT GTGTGGACAC TGATGAATGT TCGGTAGGCA ATCCTTGTGG AAATGGAACC 
TGCAAGAATG TGATTGGAGG TTTCGAATGC ACCTGTGAGG AGGGATTTGA GCCTGGGCCA 
ATGATGACTT GTGAAGACAT AAATGAGTGT GCCCAGAATC CTCTGCTCTG TGCCTTCCGC 
TGTGTAAATA CTTATGGGTC ATATGAATGC AAGTGTCCTG CTGGATATGT TCTGAGAGAA 
GACAGAAGGA TGTGCAAAGA TGAGGATGAG TGTGAAGAGG GAAAACACGA CTGTGCTGAA 
AAACAAATGG AGTGCAAGAA CCTCATTGGC ACATACCTCT GCATCTGTGG ACCTGGGTAT 
CAGCGAAGAC CTGACGGAGA AGGCTGCGTA GATGAAAATG AATGTCAGAC CAAGCCTGGG 
ATCTGTGAGA ACGGGCGCTG CCTCAACACC AGGGGGAGCT ATACCTGCGA GTGCAATGAC 
GGCTTTACAG CCAGCCCCAA CCAGGATGAG TGCCTCGACA ACCGAGAAGG GTACTGCTTC 
ACGGAGGTGC TCCAGAACAT GTGTCAGATC GGCTCAAGCA ACAGGAACCC CGTCACTAAG 
TCTGAATGCT GCTGCGATGG AGGGAGAGGC TGGGGGCCCC ACTGTGAGAT CTGCCCTTTC 
CAGGGGACTG TGGCTTTCAA AAAACTCTGT CCCCATGGCC GAGGGTTTAT GACCAATGGA 
GCAGATATTG ATGAATGCAA GGTTATTCAT GATGTTTGCC GAAATGGGGA ATGTGTCAAT 
GACAGAGGAT CATATCATTG CATTTGTAAA ACTGGCTACA CTCCAGATAT AACTGGAACT 
GCCTGTGTAG ATCTAAATGA GTGCAACCAG GCTCCCAAAC CCTGCAATTT CATCTGTAAG 
AACACAGAAG GGAGTTACCA GTGTTCATGC CCGAAAGGCT ACATTCTGCA GGAGGACGGA 
AGGAGCTGCA AAGATCTTGA TGAGTGTGCA ACTAAGCAGC ACAACTGCCA GTTCCTGTGT 
GTTAACACCA TTGGCAGCTT CACATGTAAA TGTCCTCCTG GATTTACCCA ACACCATACT 
GCCTGCATTG ATAACAATGA ATGCACCTCT GACATCAACC TGTGTGGGTC AAAGGGCATT 
TGCCAGAACA CTCCTGGAAG CTTCACTTGT GAATGCCAGC GGGGATTCTC ACTTGATCCG 
ACCGGCGCCA GCTGTGAAGA TGTGGACGAG TGTGAGGGAA ACCATCGCTG CCAGCACGGC 
TGCCAGAACA TCATCGGCGG CTACAGGTGC AGCTGCCCCC AGGGCTATCT CCAGCACTAC 
CAGTGGAACC AGTGTGTTGA TGAAAATGAA TGCCTCAGTG CTCACATCTG TGGAGGAGCC 
TCCTGTCACA ACACCCTGGG GAGCTACAAG TGCATGTGCC CTGCCGGCTT CCAATACGAA 
CAGTTCAGTG GAGGTTGCCA AGACATCAAC GAATGTGGCT CTGCTCAGGC CCCCTGCAGT 
TATGGCTGTT CCAATACTGA GGGCGGCTAC CTGTGTGCCT GTCCACCTGG CTACTTCCGA 
ATAGGCCAAG GGCACTGTGT TTCTGGAATG GGCATGGGCC GGGGAAACCC AGAGCCACCT 
GCCAGCGGTG AAATGGATGA CAACTCACTC TCCCCAGAGG CTTGTTATGA GTGTAAGATC 
AACGGCTACC CCAAACGCGG CAGGAAGCGG AGAAGCGCAA ATGAAACAGA TGCCTCCAAC 
ATTGAGGATC AACCTGAGAT AGAAGCCAAT GTGAGTCTTG CAAGTTGGGA TGTTGAGAAG 
ACAGCCGTGT TCGCTTTCAA TATTTCCCAC ATCAGTAACA AGGTCCGAAT CCTAGAACTC 
CTTCCAGCCC TCACGACTCT GACGAATCAC AACAGATACT TGATTGAATC TGGAAATGAA 
AACGGCTTCT TTAAAATCAA CCAAAAGGAA GGGATCAGCT ACCTCCACTT CACAAAGAAG 
AAGCCAGTGG CTGGAACCTA TTCATTACAA ATCAGTAGTA CTCCACTTTA TAAAAAGAAA 
GAACTTAATC AGCTAGAAGA TAAATATGAC AAAGACTACC TCAGTGGTGA ACTGGGTGAT 
AATCTGAAGA TGAAAATTCA GATTTTGCTT CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776478.1
CDS	12..8627
Translation	MRRGGLLEVALGFTVLLASYTSHGADTNLEAGNVKETRANRAKRRGGGGHNALKGPNVCGSRYNAYCCPGWKTLPGGNQCIVPICRHSCGDGFCSRPNMCTCPSGQIAPSCGSRSIQHCNIRCMNGGSCSDDHCLCQKGYIGTHCGQPVCESGCLNGGRCVAPNRCACTYGFTGPQCERDYRTGPCFTVISNQMCQGQLSGIVCTKTLCCATVGRAWGHPCEMCPAQPHPCRRGFIPNIRTGACQDVDECQAIPGLCQGGNCINTVGSFECKCPAGHKFNEVSQKCEDIDECSTIPGICDGGECTNTVSSYFCKCPPGFYTSPDGTRCIDVRPGYCYTALANGRCSNQLPQSITKMQCCCDAGRCWSPGVTVAPEMCPIRATEDFNKLCSVPMVIPERPGYPPPPLGPVPPVQPVPPGFPPGPQIMIPRPPVEYPYPSREPPRVLPVNVTDYCQLFRYLCQNGRCIPTPGSYRCECNKGFQLDLRGECIDVDECEKNPCAGGECINTQGSYTCQCRPGYQSTLTRTECRDIDECLQNGRICNNGRCINTDGSFHCVCNAGFHVTRDGKNCEDMDECSIRNMCLNGMCINEDGSFKCICKPGFQLASDGRYCKDINECETLGICMNGRCVNTDGSYRCECFPGLAVGLDGRVCVDTHMRSTCYGGYKRGQCVKPLFGAVTKSECCCASTEYAFGEPCQPCPSQNSAEYQALCSSGPGITSAGSDINECALDPDICPNGICENLRGTYKCICNSGYEVDSTGKNCVDINECVLNSLLCDNGQCRNTPGSFVCTCPKGFIYKPELKTCEDIDECESSPCINGVCKNSPGSFICECSSESTLDPTKTICIETIKGTCWQTVIDGRCEININGATLKSQCCSSLGAAWGSPCTPCQVDPICGKGYSRIKGTQCEDIDECEVFPGVCKNGLCVNSKGSFKCQCPSGMTLDATGRICLDIRLETCFLRYEDEECTLPVAGRHRMDACCCSVGAAWGTEECEECPVRNTPEYEELCPRGPGFATKEITNGKRFFKDINECKMIPNLCTHGKCRNTIGSFKCRCDSGFALDSEERNCTDIDECRISPDLCGRGQCVNTPGDFECKCDEGYESGFMMMKNCMDIDECQRDPLLCRGGVCLNTEGSYRCECPPGHQLAPNISACIDINECELSAHLCPHGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETFCTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLCYDGFMASEDMKTCVDVNECDLNPNICLSGTCENTKGSFICHCDMGYSGKKGKTGCTDINECEIGAHNCDRHAVCTNTAGSFKCSCSPGWIGDGIKCTDLDECSNGTHMCSQHADCKNTMGSYRCLCKEGYTGDGFTCTDLDECSENLNLCGNGQCLNAPGGYRCECDMGFVPSADGKACEDIDECSLPNICVFGTCHNLPGLFRCECEIGYELDRSGGNCTDVNECLDPTTCISGNCVNTPGSYTCDCPPDFELNPTRVGCVDTRSGNCYLDIRPRGDNGDTACSNEIGVGVSKASCCCSLGKAWGTPCELCPPVNTSEYKILCPGGEGFRPNPITVILEDIDECQELPGLCQGGKCINTFGSFQCRCPTGYYLNEDTRVCDDVNECETPGICGPGTCYNTVGNYTCICPPDYMQVNGGNNCMDMRRSLCYRNYYADNQTCDGELLFNMTKKMCCCSYNIGRAWNKPCEQCPIPSTDEFATLCGSQRPGFVIDIYTGLPVDIDECREIPGVCENGVCINMVGSFRCECPVGFFYNDKLLVCEDIDECQNGPVCQRNAECINTAGSYRCDCKPGYRFTSTGQCNDRNECQEIPNICSHGQCIDTVGSFYCLCHTGFKTNADQTMCLDINECERDACGNGTCRNTIGSFNCRCNHGFILSHNNDCIDVDECATGNGNLCRNGQCINTVGSFQCQCNEGYEVAPDGRTCVDINECLLDPRKCAPGTCQNLDGSYRCICPPGYSLQNDKCEDIDECVEEPEICALGTCSNTEGSFKCLCPDGFSLSSTGRRCQDLRMSYCYAKFEGGKCSSPKSRNHSKQECCCALKGEGWGDPCELCPTEPDEAFRQICPYGSGIIVGPDDSAVDMDECKEPDVCKHGQCINTDGSYRCECPFGYILQGNECVDTDECSVGNPCGNGTCKNVIGGFECTCEEGFEPGPMMTCEDINECAQNPLLCAFRCVNTYGSYECKCPAGYVLREDRRMCKDEDECEEGKHDCAEKQMECKNLIGTYLCICGPGYQRRPDGEGCVDENECQTKPGICENGRCLNTRGSYTCECNDGFTASPNQDECLDNREGYCFTEVLQNMCQIGSSNRNPVTKSECCCDGGRGWGPHCEICPFQGTVAFKKLCPHGRGFMTNGADIDECKVIHDVCRNGECVNDRGSYHCICKTGYTPDITGTACVDLNECNQAPKPCNFICKNTEGSYQCSCPKGYILQEDGRSCKDLDECATKQHNCQFLCVNTIGSFTCKCPPGFTQHHTACIDNNECTSDINLCGSKGICQNTPGSFTCECQRGFSLDPTGASCEDVDECEGNHRCQHGCQNIIGGYRCSCPQGYLQHYQWNQCVDENECLSAHICGGASCHNTLGSYKCMCPAGFQYEQFSGGCQDINECGSAQAPCSYGCSNTEGGYLCACPPGYFRIGQGHCVSGMGMGRGNPEPPASGEMDDNSLSPEACYECKINGYPKRGRKRRSANETDASNIEDQPEIEANVSLASWDVEKTAVFAFNISHISNKVRILELLPALTTLTNHNRYLIESGNENGFFKINQKEGISYLHFTKKKPVAGTYSLQISSTPLYKKKELNQLEDKYDKDYLSGELGDNLKMKIQILLH

Target ORF information:

RefSeq Version	NM_174053.2
Organism	Bos taurus(cattle)
Definition	Bos taurus fibrillin 1 (FBN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174053.2

ORF Insert Sequence:

ATGCGGCGAG GGGGGCTGCT GGAGGTCGCC TTGGGATTTA CCGTGCTCTT AGCGTCCTAC 
ACGAGCCATG GGGCGGATAC CAATTTGGAG GCTGGGAACG TGAAGGAAAC CAGAGCCAAC 
CGGGCCAAGA GAAGAGGTGG CGGAGGACAC AACGCGCTTA AAGGACCCAA TGTCTGTGGA 
TCACGTTATA ATGCTTACTG TTGCCCTGGA TGGAAGACTT TACCTGGTGG AAATCAGTGT 
ATTGTCCCCA TTTGCCGGCA TTCCTGTGGG GATGGATTTT GTTCGAGGCC AAATATGTGC 
ACTTGCCCAT CTGGTCAGAT AGCTCCTTCC TGTGGCTCCA GATCCATCCA ACATTGTAAC 
ATTCGCTGCA TGAATGGAGG TAGCTGCAGT GATGATCATT GTCTGTGCCA GAAAGGATAC 
ATAGGGACTC ACTGTGGACA ACCCGTCTGT GAAAGTGGCT GTCTTAACGG AGGCAGGTGC 
GTGGCCCCGA ATCGATGTGC GTGCACTTAC GGCTTTACTG GACCCCAGTG TGAAAGAGAT 
TACAGGACAG GTCCATGTTT TACTGTGATC AGCAACCAGA TGTGCCAGGG ACAGCTCAGC 
GGGATTGTCT GCACGAAAAC GCTCTGCTGT GCCACGGTCG GCCGTGCCTG GGGCCACCCC 
TGTGAGATGT GTCCCGCCCA GCCCCACCCC TGTCGCCGCG GCTTCATTCC AAATATCCGC 
ACAGGAGCTT GTCAAGATGT GGACGAATGC CAGGCCATCC CTGGGCTCTG TCAAGGAGGA 
AATTGCATTA ATACTGTTGG GTCTTTTGAG TGCAAATGCC CTGCTGGACA CAAATTTAAT 
GAAGTGTCAC AAAAATGTGA AGATATTGAC GAATGTAGCA CTATTCCTGG AATCTGTGAT 
GGGGGCGAAT GTACGAACAC AGTCAGCAGT TACTTTTGCA AATGTCCCCC TGGTTTTTAC 
ACCTCTCCAG ACGGTACCAG ATGCATAGAT GTCCGCCCAG GATACTGTTA CACGGCTCTG 
GCAAATGGGC GCTGTTCTAA CCAGCTGCCA CAGTCCATCA CCAAGATGCA GTGCTGCTGT 
GATGCCGGCC GGTGCTGGTC TCCGGGGGTC ACGGTCGCCC CCGAGATGTG TCCCATCAGA 
GCAACTGAGG ATTTCAACAA GCTGTGCTCC GTCCCTATGG TAATTCCCGA GAGGCCAGGA 
TATCCTCCTC CACCCCTTGG CCCCGTTCCT CCCGTTCAGC CTGTCCCTCC TGGCTTTCCT 
CCTGGGCCTC AAATCATGAT CCCTCGACCA CCAGTGGAAT ATCCGTATCC ATCGCGGGAA 
CCACCAAGGG TGCTGCCAGT CAACGTCACT GATTACTGCC AGCTCTTTCG CTATCTCTGT 
CAAAATGGAC GCTGCATCCC AACCCCCGGG AGCTACCGGT GTGAGTGCAA CAAAGGATTC 
CAGCTGGACC TCCGTGGGGA GTGCATTGAT GTTGATGAAT GTGAGAAGAA CCCTTGTGCT 
GGTGGCGAGT GTATTAACAC GCAGGGCTCT TACACCTGTC AGTGCCGACC TGGTTATCAG 
AGCACACTCA CGCGGACAGA GTGCCGAGAC ATTGATGAAT GTTTACAGAA TGGCCGTATC 
TGTAATAATG GACGTTGTAT CAACACGGAT GGCAGTTTCC ATTGTGTGTG TAATGCAGGA 
TTTCACGTCA CACGAGATGG GAAGAACTGT GAAGATATGG ATGAATGCAG CATAAGGAAT 
ATGTGCCTTA ATGGAATGTG TATCAACGAA GATGGCAGTT TCAAATGTAT TTGCAAACCT 
GGATTTCAGC TGGCATCAGA TGGGCGTTAT TGTAAAGATA TTAATGAGTG TGAAACCCTT 
GGGATCTGCA TGAACGGGCG TTGCGTCAAC ACTGATGGCT CCTACAGATG TGAATGCTTT 
CCTGGACTGG CCGTGGGTCT GGACGGACGT GTGTGTGTTG ACACACACAT GCGGAGCACA 
TGCTATGGTG GATACAAAAG AGGCCAGTGT GTCAAACCCT TGTTTGGTGC TGTTACCAAG 
TCTGAATGCT GTTGTGCCAG CACTGAGTAT GCTTTCGGGG AACCTTGCCA ACCATGTCCT 
TCACAGAATT CAGCGGAGTA TCAGGCGCTC TGCAGCAGTG GGCCAGGAAT AACGTCAGCA 
GGCAGCGATA TAAATGAATG TGCATTAGAT CCTGATATTT GCCCAAATGG AATTTGTGAA 
AATCTTCGTG GGACCTACAA ATGTATATGC AATTCAGGAT ATGAAGTGGA TTCAACTGGG 
AAAAACTGTG TTGATATTAA TGAATGTGTA TTGAATAGTC TGCTTTGTGA CAATGGACAA 
TGTAGAAATA CTCCTGGAAG TTTTGTCTGT ACCTGCCCCA AGGGATTTAT ATACAAACCT 
GAACTGAAAA CCTGTGAAGA CATCGATGAA TGTGAATCAA GTCCTTGCAT TAATGGAGTC 
TGCAAGAACA GTCCGGGCTC TTTCATTTGT GAATGTTCTT CTGAAAGTAC TTTGGATCCA 
ACAAAAACCA TCTGCATAGA AACCATTAAG GGCACTTGTT GGCAGACTGT CATCGATGGG 
CGGTGTGAGA TCAACATCAA TGGAGCCACA TTGAAGTCCC AGTGCTGTTC ATCCCTTGGT 
GCTGCCTGGG GGAGCCCATG CACCCCATGC CAAGTCGATC CCATATGTGG GAAAGGCTAT 
TCAAGAATTA AAGGAACACA GTGTGAAGAT ATAGATGAAT GTGAAGTGTT CCCAGGAGTC 
TGCAAGAATG GCTTGTGTGT TAACTCCAAG GGATCATTTA AATGTCAGTG TCCCAGTGGG 
ATGACTTTGG ATGCCACAGG GAGGATTTGC CTAGATATCC GCCTGGAAAC CTGCTTCCTG 
AGGTACGAGG ACGAGGAGTG CACCCTGCCC GTGGCTGGGC GCCACCGCAT GGACGCCTGC 
TGCTGCTCAG TCGGGGCAGC CTGGGGCACC GAGGAGTGTG AGGAGTGTCC CGTGAGAAAC 
ACACCTGAGT ACGAGGAGCT CTGTCCCAGA GGACCCGGAT TCGCCACAAA AGAAATTACA 
AATGGCAAAC GTTTTTTCAA AGACATCAAC GAGTGTAAGA TGATTCCCAA CCTGTGCACC 
CACGGCAAGT GCAGGAACAC CATTGGCAGC TTCAAGTGCA GGTGTGACAG CGGCTTTGCT 
CTGGATTCTG AAGAAAGGAA CTGCACAGAC ATCGACGAAT GCCGCATATC CCCTGACCTC 
TGTGGCCGAG GCCAGTGTGT GAACACCCCC GGGGACTTTG AGTGCAAGTG CGATGAAGGC 
TATGAAAGCG GATTCATGAT GATGAAGAAC TGCATGGATA TCGATGAGTG TCAGAGAGAT 
CCGCTGTTGT GCCGAGGAGG CGTGTGCCTG AACACTGAGG GAAGTTACCG CTGTGAATGT 
CCCCCTGGCC ATCAGCTGGC CCCCAACATC TCGGCGTGTA TAGACATCAA CGAGTGTGAA 
CTAAGTGCAC ACCTCTGCCC CCATGGCCGC TGCGTGAACC TCATAGGAAA GTACCAGTGT 
GCCTGCAACC CTGGCTACCA CTCTACCCCT GACAGGCTGT TTTGTGTTGA CATTGATGAA 
TGCAGCATAA TGAACGGTGG CTGTGAGACC TTCTGCACAA ACTCTGAAGG TAGCTACGAA 
TGTAGCTGTC AGCCAGGATT TGCACTGATG CCTGACCAGA GGTCCTGCAC AGACATTGAT 
GAGTGTGAAG ATAACCCCAA TATCTGTGAT GGTGGTCAGT GTACAAACAT ACCTGGAGAG 
TACAGGTGCT TGTGTTATGA TGGATTCATG GCATCTGAAG ACATGAAGAC TTGCGTAGAT 
GTCAATGAAT GTGACCTGAA TCCAAATATC TGCCTAAGTG GAACCTGTGA AAACACCAAA 
GGCTCATTTA TCTGCCACTG TGATATGGGC TATTCAGGCA AAAAAGGAAA AACAGGCTGT 
ACAGACATCA ATGAATGTGA AATTGGAGCA CACAACTGCG ACAGACATGC TGTATGTACC 
AACACAGCAG GAAGCTTCAA ATGCAGCTGT AGCCCCGGGT GGATTGGAGA TGGCATTAAG 
TGCACTGACC TGGACGAATG CTCCAATGGA ACCCACATGT GCAGCCAACA TGCAGACTGC 
AAGAACACCA TGGGCTCCTA CCGCTGCCTC TGCAAAGAGG GGTACACGGG CGATGGCTTC 
ACTTGTACAG ATCTTGATGA GTGCTCTGAG AATCTAAACC TGTGTGGCAA CGGCCAGTGC 
CTTAACGCCC CAGGAGGATA CCGCTGTGAA TGTGACATGG GCTTTGTGCC CAGTGCTGAT 
GGGAAAGCCT GTGAAGATAT CGATGAGTGC TCTCTTCCGA ACATCTGTGT TTTTGGAACT 
TGCCACAACC TCCCTGGCCT CTTCCGCTGT GAATGTGAGA TCGGCTATGA ACTGGACAGA 
AGTGGTGGGA ACTGCACAGA CGTTAATGAA TGCCTGGATC CAACCACGTG CATCAGTGGG 
AACTGTGTCA ATACTCCAGG CAGCTATACC TGTGACTGTC CACCCGATTT TGAGCTGAAT 
CCAACTCGAG TTGGCTGTGT CGATACCCGC TCTGGAAACT GCTATTTGGA TATCCGTCCT 
CGGGGAGACA ATGGAGATAC AGCCTGCAGC AATGAAATTG GGGTTGGAGT GTCCAAGGCT 
TCTTGCTGCT GCTCTCTGGG TAAAGCCTGG GGCACTCCTT GTGAGCTGTG CCCGCCTGTG 
AACACATCTG AGTACAAAAT TCTTTGTCCT GGAGGAGAAG GTTTTCGACC CAACCCTATC 
ACCGTTATAT TGGAAGATAT TGATGAGTGC CAGGAGCTTC CAGGGTTGTG CCAAGGCGGG 
AAATGTATCA ATACCTTCGG CAGCTTCCAG TGCCGCTGTC CAACGGGCTA CTACCTGAAT 
GAAGATACCC GAGTGTGTGA TGATGTGAAT GAATGTGAAA CTCCCGGAAT CTGTGGTCCA 
GGGACATGTT ACAACACTGT TGGCAACTAC ACCTGTATCT GCCCTCCAGA CTACATGCAA 
GTGAATGGGG GGAATAATTG CATGGATATG AGGAGGAGTC TGTGCTACAG GAACTATTAT 
GCTGACAACC AGACCTGCGA TGGAGAACTG TTGTTCAACA TGACCAAGAA GATGTGCTGT 
TGTTCCTACA ACATTGGCCG GGCCTGGAAC AAGCCCTGTG AACAGTGCCC CATTCCAAGC 
ACAGATGAAT TTGCTACACT CTGTGGAAGT CAACGGCCAG GCTTTGTCAT CGACATCTAT 
ACCGGTTTAC CTGTCGATAT CGACGAGTGT CGGGAGATCC CAGGGGTCTG TGAAAACGGT 
GTGTGTATCA ACATGGTCGG CAGCTTCCGT TGTGAATGTC CAGTGGGCTT CTTCTATAAT 
GACAAGTTGC TGGTTTGTGA AGACATTGAC GAGTGTCAGA ACGGCCCAGT GTGCCAGCGA 
AACGCAGAGT GCATCAACAC CGCGGGCAGC TACCGCTGTG ATTGCAAACC CGGCTACCGA 
TTCACCTCCA CGGGGCAGTG CAATGATCGC AATGAATGTC AAGAAATCCC CAATATATGC 
AGCCATGGAC AGTGTATTGA CACAGTTGGA AGCTTTTATT GCCTTTGCCA CACTGGTTTT 
AAAACAAACG CTGATCAAAC CATGTGTTTG GACATAAATG AGTGTGAAAG AGATGCCTGT 
GGGAATGGCA CTTGCCGGAA CACGATTGGT TCCTTCAACT GCCGCTGCAA TCACGGTTTC 
ATCCTTTCCC ACAACAATGA CTGCATAGAT GTTGATGAAT GTGCAACTGG AAATGGGAAC 
CTTTGCAGAA ATGGCCAGTG TATCAATACA GTGGGGTCCT TCCAGTGTCA GTGCAATGAA 
GGCTATGAGG TGGCTCCAGA TGGGAGAACC TGTGTGGATA TCAATGAATG TCTCCTAGAT 
CCCAGAAAAT GTGCCCCAGG TACCTGTCAA AACTTGGATG GGTCCTACAG ATGTATCTGT 
CCACCTGGAT ACAGTCTCCA GAATGACAAG TGTGAAGATA TTGATGAATG TGTTGAAGAG 
CCAGAAATCT GTGCCTTGGG CACATGCAGT AACACTGAAG GCAGCTTCAA ATGTCTGTGT 
CCAGACGGGT TCTCCTTGTC CTCCACTGGA AGAAGGTGCC AAGATTTACG AATGAGCTAC 
TGTTACGCGA AGTTTGAAGG AGGAAAGTGT TCATCACCCA AATCCAGAAA TCACTCCAAA 
CAGGAATGCT GCTGTGCCTT GAAGGGAGAA GGTTGGGGAG ATCCCTGCGA GCTGTGCCCC 
ACTGAACCCG ATGAGGCCTT CCGCCAGATC TGTCCCTATG GAAGTGGCAT CATCGTGGGA 
CCTGATGATT CAGCAGTTGA CATGGATGAG TGCAAAGAAC CCGATGTCTG TAAACACGGG 
CAGTGCATCA ACACCGATGG GTCCTATCGC TGCGAATGTC CCTTTGGCTA CATTCTGCAA 
GGGAATGAAT GTGTGGACAC TGATGAATGT TCGGTAGGCA ATCCTTGTGG AAATGGAACC 
TGCAAGAATG TGATTGGAGG TTTCGAATGC ACCTGTGAGG AGGGATTTGA GCCTGGGCCA 
ATGATGACTT GTGAAGACAT AAATGAGTGT GCCCAGAATC CTCTGCTCTG TGCCTTCCGC 
TGTGTAAATA CTTATGGGTC ATATGAATGC AAGTGTCCTG CTGGATATGT TCTGAGAGAA 
GACAGAAGGA TGTGCAAAGA TGAGGATGAG TGTGAAGAGG GAAAACACGA CTGTGCTGAA 
AAACAAATGG AGTGCAAGAA CCTCATTGGC ACATACCTCT GCATCTGTGG ACCTGGGTAT 
CAGCGAAGAC CTGACGGAGA AGGCTGCGTA GATGAAAATG AATGTCAGAC CAAGCCTGGG 
ATCTGTGAGA ACGGGCGCTG CCTCAACACC AGGGGGAGCT ATACCTGCGA GTGCAATGAC 
GGCTTTACAG CCAGCCCCAA CCAGGATGAG TGCCTCGACA ACCGAGAAGG GTACTGCTTC 
ACGGAGGTGC TCCAGAACAT GTGTCAGATC GGCTCAAGCA ACAGGAACCC CGTCACTAAG 
TCTGAATGCT GCTGCGATGG AGGGAGAGGC TGGGGGCCCC ACTGTGAGAT CTGCCCTTTC 
CAGGGGACTG TGGCTTTCAA AAAACTCTGT CCCCATGGCC GAGGGTTTAT GACCAATGGA 
GCAGATATTG ATGAATGCAA GGTTATTCAT GATGTTTGCC GAAATGGGGA ATGTGTCAAT 
GACAGAGGAT CATATCATTG CATTTGTAAA ACTGGCTACA CTCCAGATAT AACTGGAACT 
GCCTGTGTAG ATCTAAATGA GTGCAACCAG GCTCCCAAAC CCTGCAATTT CATCTGTAAG 
AACACAGAAG GGAGTTACCA GTGTTCATGC CCGAAAGGCT ACATTCTGCA GGAGGACGGA 
AGGAGCTGCA AAGATCTTGA TGAGTGTGCA ACTAAGCAGC ACAACTGCCA GTTCCTGTGT 
GTTAACACCA TTGGCAGCTT CACATGTAAA TGTCCTCCTG GATTTACCCA ACACCATACT 
GCCTGCATTG ATAACAATGA ATGCACCTCT GACATCAACC TGTGTGGGTC AAAGGGCATT 
TGCCAGAACA CTCCTGGAAG CTTCACTTGT GAATGCCAGC GGGGATTCTC ACTTGATCCG 
ACCGGCGCCA GCTGTGAAGA TGTGGACGAG TGTGAGGGAA ACCATCGCTG CCAGCACGGC 
TGCCAGAACA TCATCGGCGG CTACAGGTGC AGCTGCCCCC AGGGCTATCT CCAGCACTAC 
CAGTGGAACC AGTGTGTTGA TGAAAATGAA TGCCTCAGTG CTCACATCTG TGGAGGAGCC 
TCCTGTCACA ACACCCTGGG GAGCTACAAG TGCATGTGCC CTGCCGGCTT CCAATACGAA 
CAGTTCAGTG GAGGTTGCCA AGACATCAAC GAATGTGGCT CTGCTCAGGC CCCCTGCAGT 
TATGGCTGTT CCAATACTGA GGGCGGCTAC CTGTGTGCCT GTCCACCTGG CTACTTCCGA 
ATAGGCCAAG GGCACTGTGT TTCTGGAATG GGCATGGGCC GGGGAAACCC AGAGCCACCT 
GCCAGCGGTG AAATGGATGA CAACTCACTC TCCCCAGAGG CTTGTTATGA GTGTAAGATC 
AACGGCTACC CCAAACGCGG CAGGAAGCGG AGAAGCGCAA ATGAAACAGA TGCCTCCAAC 
ATTGAGGATC AACCTGAGAT AGAAGCCAAT GTGAGTCTTG CAAGTTGGGA TGTTGAGAAG 
ACAGCCGTGT TCGCTTTCAA TATTTCCCAC ATCAGTAACA AGGTCCGAAT CCTAGAACTC 
CTTCCAGCCC TCACGACTCT GACGAATCAC AACAGATACT TGATTGAATC TGGAAATGAA 
AACGGCTTCT TTAAAATCAA CCAAAAGGAA GGGATCAGCT ACCTCCACTT CACAAAGAAG 
AAGCCAGTGG CTGGAACCTA TTCATTACAA ATCAGTAGTA CTCCACTTTA TAAAAAGAAA 
GAACTTAATC AGCTAGAAGA TAAATATGAC AAAGACTACC TCAGTGGTGA ACTGGGTGAT 
AATCTGAAGA TGAAAATTCA GATTTTGCTT CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBN1 ( XM_015473179.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328665.1 Bos taurus fibrillin 1 (FBN1), transcript variant X1, mRNA.

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CloneID	OBa91701
Clone ID Related Accession (Same CDS sequence)	XM_015473179.1 , XM_015473179.2
Accession Version	XM_015473179.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	fibrillin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGAG GGGGGCTGCT GGAGGTCGCC CTGGGATTTA CCGTGCTCTT AGCGTCCTAC 
ACGAGCCATG GGGCGGATAC CAATTTGGAG GCTGGGAACG TGAAGGAAAC CAGAGCCAAC 
CGGGCCAAGA GAAGAGGTGG CGGAGGACAC GACGCGCTTA AAGGACCCAA TGTCTGTGGA 
TCACGTTATA ATGCTTACTG TTGCCCTGGA TGGAAGACTT TACCTGGTGG AAATCAGTGT 
ATTGTCCCCA TTTGCCGGCA TTCCTGTGGG GATGGATTTT GTTCGAGGCC AAATATGTGC 
ACTTGCCCAT CTGGTCAGAT AGCTCCTTCC TGTGGCTCCA GATCCATCCA ACATTGTAAC 
ATTCGCTGCA TGAATGGAGG TAGCTGCAGT GATGATCATT GTCTGTGCCA GAAAGGATAC 
ATAGGGACTC ACTGTGGACA ACCCGTCTGT GAAAGTGGCT GTCTTAACGG AGGCAGGTGC 
GTGGCCCCGA ATCGATGTGC GTGCACTTAC GGCTTTACTG GACCCCAGTG TGAAAGAGAT 
TACAGGACAG GTCCATGTTT TACTGTGATC AGCAACCAGA TGTGCCAGGG ACAGCTCAGC 
GGGATTGTCT GCACGAAAAC GCTCTGCTGT GCCACGGTCG GCCGTGCCTG GGGCCACCCC 
TGTGAGATGT GTCCCGCCCA GCCCCACCCC TGTCGCCGCG GCTTCATTCC AAATATCCGC 
ACAGGAGCTT GTCAAGATGT GGACGAATGC CAGGCCATCC CTGGGCTCTG TCAAGGAGGA 
AATTGCATTA ATACTGTTGG GTCTTTTGAG TGCAAATGCC CTGCTGGACA CAAATTTAAT 
GAAGTGTCAC AAAAATGTGA AGATATTGAC GAATGTAGCA CTATTCCTGG AATCTGTGAT 
GGGGGCGAAT GTACGAACAC AGTCAGCAGT TACTTTTGCA AATGTCCCCC TGGTTTTTAC 
ACCTCTCCAG ACGGTACCAG ATGCATAGAT GTCCGCCCAG GATACTGTTA CACGGCTCTG 
GCAAATGGGC GCTGTTCTAA CCAGCTGCCA CAGTCCATCA CCAAGATGCA GTGCTGCTGT 
GACGTCGGCC GGTGCTGGTC TCCCGGGGTC ACGGTCGCCC CCGAGATGTG TCCCATCAGA 
GCAACTGAGG ATTTCAACAA GCTGTGCTCC GTCCCTATGG TAATTCCCGA GAGGCCAGGA 
TATCCTCCTC CACCCCTTGG CCCCGTTCCT CCCGTTCAGC CTGTCCCTCC TGGCTTTCCT 
CCTGGGCCTC AAATCATGAT CCCTCGACCA CCAGTGGAAT ATCCGTATCC ATCGCGGGAA 
CCACCAAGGG TGCTGCCAGT CAACGTCACT GATTACTGCC AGCTCTTTCG CTATCTCTGT 
CAAAATGGAC GCTGCATCCC AACCCCCGGG AGCTACCGGT GTGAGTGCAA CAAAGGATTC 
CAGCTGGACC TCCGTGGGGA GTGCATTGAT GTTGATGAAT GTGAGAAGAA CCCTTGTGCT 
GGTGGCGAGT GTATTAACAC GCAGGGCTCT TACACCTGTC AGTGCCGACC TGGTTATCAG 
AGCACACTCA CGCGGACAGA GTGCCGAGAC ATTGATGAAT GTTTACAGAA TGGCCGTATC 
TGTAATAATG GACGTTGTAT CAACACGGAT GGCAGTTTCC ATTGTGTGTG TAATGCAGGA 
TTTCACGTCA CACGAGATGG GAAGAACTGT GAAGATATGG ATGAATGCAG CATAAGGAAT 
ATGTGCCTTA ATGGAATGTG TATCAACGAA GATGGCAGTT TCAAATGTAT TTGCAAACCT 
GGATTTCAGC TGGCATCAGA TGGGCGTTAT TGTAAAGATA TTAATGAGTG TGAAACACCT 
GGGATCTGCA TGAATGGGCG TTGCGTCAAC ACTGATGGCT CCTACCGATG TGAATGCTTT 
CCTGGACTGG CCGTGGGTCT GGACGGACGT GTGTGTGTTG ACACACACAT GCGGAGCACA 
TGCTATGGTG GATACAAAAG AGGCCAGTGT GTCAAACCCT TGTTTGGTGC TGTTACCAAG 
TCTGAATGCT GTTGTGCCAG CACTGAGTAT GCTTTCGGGG AACCTTGCCA ACCATGTCCT 
TCACAGAATT CAGCGGAGTA TCAGGCGCTC TGCAGCAGTG GGCCAGGAAT AACGTCAGCA 
GGCAGCGATA TAAATGAATG TGCATTAGAT CCTGATATTT GCCCAAATGG AATTTGTGAA 
AATCTCCGTG GGACCTACAA ATGTATATGC AACTCAGGAT ATGAAGTGGA TTCAACTGGG 
AAAAACTGTG TTGATATTAA TGAATGTGTA TTGAATAGTC TGCTTTGTGA CAATGGACAA 
TGTAGAAATA CTCCTGGAAG TTTTGTCTGT ACCTGCCCCA AGGGATTTAT ATACAAACCT 
GAACTGAAAA CCTGTGAAGA CATCGATGAA TGTGAATCAA GTCCTTGCAT TAATGGAGTC 
TGCAAGAACA GTCCGGGCTC TTTCATTTGT GAATGTTCTT CTGAAAGTAC TTTGGATCCA 
ACAAAAACCA TCTGCATAGA AACCATTAAG GGCACTTGTT GGCAGACTGT CATCGATGGG 
CGGTGTGAGA TCAACATCAA TGGAGCCACA TTGAAGTCCC AGTGCTGTTC ATCCCTTGGT 
GCTGCCTGGG GGAGCCCATG CACCCCATGC CAAGTCGATC CCATATGTGG GAAAGGCTAT 
TCAAGAATTA AAGGAACACA GTGTGAAGAT ATAGATGAAT GTGAAGTGTT CCCAGGAGTC 
TGCAAGAATG GCTTGTGTGT TAACTCCAAG GGATCATTTA AATGTCAGTG TCCCAGTGGG 
ATGACTTTGG ATGCCACAGG GAGGATTTGC CTAGATATCC GCCTGGAAAC CTGCTTCCTG 
AGGTACGAGG ACGAGGAGTG CACCCTGCCC GTGGCTGGGC GCCACCGCAT GGACGCCTGC 
TGCTGCTCAG TCGGGGCAGC CTGGGGCACC GAGGAGTGTG AGGAGTGTCC CGTGAGAAAC 
ACACCTGAGT ACGAGGAGCT CTGTCCCAGA GGACCCGGAT TCGCCACAAA AGAAATTACA 
AATGGCAAAC GTTTTTTCAA AGACATCAAC GAGTGTAAGA TGATTCCCAA CCTGTGCACC 
CACGGCAAGT GCAGGAACAC CATTGGCAGC TTCAAGTGCA GGTGTGACAG CGGCTTTGCT 
CTGGATTCTG AAGAAAGGAA CTGCACAGAC ATCGACGAAT GCCGCATATC CCCTGACCTC 
TGTGGCCGAG GCCAGTGTGT GAACACCCCC GGGGACTTTG AGTGCAAGTG CGATGAAGGC 
TATGAAAGCG GATTCATGAT GATGAAGAAC TGCATGGATA TCGATGAGTG TCAGAGAGAT 
CCGCTGTTGT GCCGAGGAGG CGTGTGCCTG AACACTGAGG GAAGTTACCG CTGTGAATGT 
CCCCCTGGCC ATCAGCTGGC CCCCAACATC TCGGCGTGTA TAGACATCAA CGAGTGTGAA 
CTAAGTGCAC ACCTCTGCCC CCATGGCCGC TGCGTGAACC TCATAGGAAA GTACCAGTGT 
GCCTGCAACC CTGGCTACCA CTCTACCCCT GACAGGCTGT TTTGTGTTGA CATTGATGAA 
TGCAGCATAA TGAACGGTGG CTGTGAGACC TTCTGCACAA ACTCTGAAGG TAGCTACGAA 
TGTAGCTGTC AGCCAGGATT TGCACTGATG CCTGACCAGA GGTCCTGCAC AGACATTGAT 
GAGTGTGAAG ATAACCCCAA TATCTGTGAT GGTGGTCAGT GTACAAACAT ACCTGGAGAG 
TACAGGTGCT TGTGTTATGA TGGATTCATG GCATCTGAAG ACATGAAGAC TTGCGTAGAT 
GTCAATGAAT GTGATCTGAA TCCAAATATC TGCCTAAGTG GAACCTGTGA AAACACCAAA 
GGCTCATTTA TCTGCCACTG TGATATGGGC TATTCAGGCA AAAAAGGAAA AACAGGCTGT 
ACAGACATCA ATGAATGTGA AATTGGAGCA CACAACTGCG ACAGACATGC TGTATGTACC 
AACACAGCAG GAAGCTTCAA ATGCAGCTGT AGCCCCGGGT GGATTGGAGA TGGCATTAAG 
TGCACTGACC TGGACGAATG CTCCAATGGA ACCCACATGT GCAGCCAACA TGCAGACTGC 
AAGAACACCA TGGGCTCCTA CCGCTGCCTC TGCAAAGAGG GGTACACAGG CGATGGCTTC 
ACTTGTACAG ATCTTGATGA GTGCTCTGAG AATCTAAACC TGTGTGGCAA CGGCCAGTGC 
CTTAACGCCC CAGGAGGATA CCGCTGTGAA TGTGACATGG GCTTTGTGCC CAGTGCTGAT 
GGGAAAGCCT GTGAAGATAT CGATGAGTGC TCTCTTCCGA ACATCTGTGT TTTTGGAACT 
TGCCACAACC TCCCTGGCCT CTTCCGCTGT GAATGTGAGA TCGGCTATGA ACTGGACAGA 
AGTGGTGGGA ACTGCACAGA CGTTAATGAA TGCCTGGATC CAACCACGTG CATCAGTGGG 
AACTGTGTCA ATACTCCAGG CAGCTATACC TGTGACTGTC CACCCGATTT TGAGCTGAAT 
CCAACTCGAG TTGGCTGTGT CGATACCCGC TCTGGAAACT GCTATTTGGA TATCCGTCCT 
CGGGGAGACA ATGGAGATAC AGCCTGCAGC AATGAAATTG GGGTTGGAGT GTCCAAGGCT 
TCCTGCTGCT GCTCTCTGGG TAAAGCCTGG GGCACTCCTT GTGAGCTGTG CCCGCCTGTG 
AACACATCTG AGTACAAAAT TCTTTGTCCT GGAGGAGAAG GTTTTCGACC CAACCCTATC 
ACCGTTATAT TGGAAGATAT TGATGAGTGC CAGGAGCTTC CAGGGTTGTG CCAAGGCGGG 
AAATGTATCA ATACCTTCGG CAGCTTCCAG TGCCGCTGTC CAACGGGCTA CTACCTGAAT 
GAAGATACCC GAGTGTGTGA TGATGTGAAT GAATGTGAAA CTCCCGGAAT CTGTGGTCCA 
GGGACATGTT ACAACACTGT TGGCAACTAC ACCTGTATCT GCCCTCCAGA CTACATGCAA 
GTGAATGGGG GGAATAATTG CATGGATATG AGGAGGAGTC TGTGCTACAG GAACTATTAT 
GCTGACAACC AGACCTGCGA TGGAGAACTG TTGTTCAACA TGACCAAGAA GATGTGCTGT 
TGTTCCTACA ACATTGGCCG GGCCTGGAAC AAGCCCTGTG AACAGTGCCC CATTCCAAGC 
ACAGATGAAT TTGCTACACT CTGTGGAAGT CAACGGCCAG GCTTTGTCAT CGACATCTAT 
ACCGGTTTAC CTGTCGATAT CGACGAGTGT CGGGAGATCC CAGGGGTCTG TGAAAACGGT 
GTGTGTATCA ACATGGTCGG CAGCTTCCGT TGTGAATGTC CAGTGGGCTT CTTCTATAAT 
GACAAGTTGC TGGTTTGTGA AGACATTGAC GAGTGTCAGA ACGGCCCAGT GTGCCAGCGA 
AACGCAGAGT GCATCAACAC CGCGGGCAGC TACCGCTGTG ATTGCAAACC CGGCTACCGA 
TTCACCTCCA CGGGGCAGTG CAATGATCGC AATGAATGTC AAGAAATCCC CAATATATGC 
AGCCATGGAC AGTGTATTGA CACAGTTGGA AGCTTTTATT GCCTTTGCCA CACTGGTTTT 
AAAACAAACG CTGATCAAAC CATGTGTTTG GACATAAATG AGTGTGAAAG AGATGCCTGT 
GGGAATGGCA CTTGCCGCAA CACGATTGGT TCCTTCAACT GCCGCTGCAA TCACGGTTTC 
ATCCTTTCCC ACAACAATGA CTGCATAGAT GTTGATGAAT GTGCAACTGG AAATGGGAAC 
CTTTGCAGAA ATGGCCAGTG TATCAATACA GTGGGGTCCT TCCAGTGTCA GTGCAATGAA 
GGCTATGAGG TGGCTCCAGA TGGGAGAACC TGTGTGGATA TCAATGAATG TCTCCTAGAT 
CCCAGAAAAT GTGCCCCAGG CACCTGTCAA AACTTGGATG GGTCCTACAG ATGTATCTGT 
CCACCTGGAT ACAGTCTCCA GAATGACAAG TGTGAAGATA TTGATGAATG TGTTGAAGAG 
CCAGAAATCT GTGCCTTGGG CACGTGCAGT AACACTGAAG GCAGCTTCAA ATGTCTGTGT 
CCAGACGGGT TCTCCTTGTC CTCCACTGGA AGAAGGTGCC AAGATTTACG AATGAGCTAC 
TGTTACGCGA AGTTTGAAGG AGGAAAGTGT TCATCACCCA AATCCAGAAA TCACTCCAAA 
CAGGAATGCT GCTGTGCCTT GAAGGGAGAA GGTTGGGGAG ATCCCTGCGA GCTGTGCCCC 
ACTGAACCCG ATGAGGCCTT CCGCCAGATC TGTCCCTATG GAAGTGGCAT CATCGTGGGA 
CCTGATGATT CAGCAGTTGA CATGGATGAG TGCAAAGAAC CCGATGTCTG TAAACACGGG 
CAGTGCATCA ACACCGATGG GTCCTATCGC TGCGAATGTC CCTTTGGCTA CATTCTGCAA 
GGGAATGAAT GTGTGGACAC TGATGAATGT TCGGTAGGCA ATCCTTGTGG AAATGGAACC 
TGCAAGAATG TGATTGGAGG TTTCGAATGC ACCTGTGAGG AGGGATTTGA GCCTGGGCCA 
ATGATGACTT GTGAAGACAT AAATGAGTGT GCCCAGAATC CTCTGCTCTG TGCCTTCCGC 
TGTGTAAATA CTTATGGGTC ATATGAATGC AAGTGTCCTG CTGGATATGT TCTGAGAGAA 
GACAGAAGGA TGTGCAAAGA TGAGGATGAG TGTGAAGAGG GAAAACACGA CTGTGCTGAA 
AAACAAATGG AGTGCAAGAA CCTCATTGGC ACATACCTCT GCATCTGTGG ACCTGGGTAT 
CAGCGAAGAC CTGACGGAGA AGGCTGCGTA GATGAAAATG AATGTCAGAC CAAGCCTGGG 
ATCTGTGAGA ACGGGCGCTG CCTCAACACC AGGGGGAGCT ATACCTGCGA GTGCAATGAC 
GGCTTTACAG CCAGCCCCAA CCAGGATGAG TGCCTCGACA ACCGAGAAGG GTACTGCTTC 
ACGGAGGTGC TCCAGAACAT GTGTCAGATC GGCTCAAGCA ACAGGAACCC CGTCACTAAG 
TCTGAATGCT GCTGCGATGG AGGGAGAGGC TGGGGGCCCC ACTGTGAGAT CTGCCCTTTC 
CAGGGCACTG TGGCTTTCAA AAAACTCTGC CCCCATGGCC GAGGGTTTAT GACCAATGGA 
GCAGATATTG ATGAGTGCAA GGTTATTCAT GATGTTTGCC GAAATGGGGA ATGTGTCAAT 
GACAGAGGAT CATATCATTG CATTTGTAAA ACTGGCTACA CTCCAGATAT AACTGGAACT 
GCCTGTGTAG ATCTAAATGA GTGCAACCAG GCTCCCAAAC CCTGCAATTT CATCTGTAAG 
AACACAGAAG GGAGTTACCA GTGTTCATGC CCGAAAGGCT ACATTCTGCA GGAGGACGGA 
AGGAGCTGCA AAGATCTTGA TGAGTGTGCA ACTAAGCAGC ACAACTGCCA GTTCCTGTGT 
GTTAACACCA TTGGCAGCTT CACATGTAAA TGTCCTCCTG GATTTACCCA ACACCATACT 
GCCTGCATTG ATAACAATGA ATGCACCTCT GACATCAACC TGTGTGGGTC AAAGGGCATT 
TGCCAGAACA CTCCTGGAAG CTTCACTTGT GAATGCCAGC GGGGATTCTC ACTTGATCCG 
AGCGGCGCAA GCTGTGAAGA TGTGGACGAG TGTGAGGGAA ACCATCGCTG CCAGCACGGC 
TGCCAGAACA TCATCGGCGG CTACAGGTGC AGCTGCCCCC AGGGCTATCT CCAGCACTAC 
CAGTGGAACC AGTGTGTTGA TGAAAATGAA TGCCTCAGTG CTCACATCTG TGGAGGAGCC 
TCCTGTCACA ACACCCTGGG GAGCTACAAG TGCATGTGCC CTGCCGGCTT CCAATACGAA 
CAGTTCAGTG GAGGTTGCCA AGACATCAAC GAATGTGGCT CTGCTCAGGC CCCCTGCAGT 
TATGGCTGTT CCAATACTGA GGGCGGCTAC CTGTGTGCCT GTCCACCTGG CTACTTCCGA 
ATAGGCCAAG GGCACTGTGT TTCTGGAATG GGCATGGGCC GGGGAAACCC AGAGCCACCT 
GCCAGCGGTG AAATGGATGA CAACTCACTC TCCCCAGAGG CTTGTTATGA GTGTAAGATC 
AACGGCTACC CCAAACGCGG CAGGAAGCGG AGAAGCGCAA ATGAAACAGA TGCCTCCAAC 
ATTGAGGATC AACCTGAGAT AGAAGCCAAT GTGAGTCTTG CAAGTTGGGA TGTTGAGAAG 
ACAGCCGTGT TCGCTTTCAA TATTTCCCAC ATCAGTAACA AGGTCCGAAT CCTAGAACTC 
CTTCCAGCCC TCACGACTCT GACGAATCAC AACAGATACT TGATTGAATC TGGAAATGAA 
AACGGCTTCT TTAAAATCAA CCAAAAGGAA GGGATCAGCT ACCTCCACTT CACAAAGAAG 
AAGCCAGTGG CTGGAACCTA TTCATTACAA ATCAGTAGTA CTCCACTTTA TAAAAAGAAA 
GAACTTAATC AGCTAGAAGA TAAATATGAC AAAGACTACC TCAGTGGTGA ACTGGGTGAT 
AATCTGAAGA TGAAAATTCA GATTTTGCTT CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328665.1
CDS	301..8916
Translation	MRRGGLLEVALGFTVLLASYTSHGADTNLEAGNVKETRANRAKRRGGGGHDALKGPNVCGSRYNAYCCPGWKTLPGGNQCIVPICRHSCGDGFCSRPNMCTCPSGQIAPSCGSRSIQHCNIRCMNGGSCSDDHCLCQKGYIGTHCGQPVCESGCLNGGRCVAPNRCACTYGFTGPQCERDYRTGPCFTVISNQMCQGQLSGIVCTKTLCCATVGRAWGHPCEMCPAQPHPCRRGFIPNIRTGACQDVDECQAIPGLCQGGNCINTVGSFECKCPAGHKFNEVSQKCEDIDECSTIPGICDGGECTNTVSSYFCKCPPGFYTSPDGTRCIDVRPGYCYTALANGRCSNQLPQSITKMQCCCDVGRCWSPGVTVAPEMCPIRATEDFNKLCSVPMVIPERPGYPPPPLGPVPPVQPVPPGFPPGPQIMIPRPPVEYPYPSREPPRVLPVNVTDYCQLFRYLCQNGRCIPTPGSYRCECNKGFQLDLRGECIDVDECEKNPCAGGECINTQGSYTCQCRPGYQSTLTRTECRDIDECLQNGRICNNGRCINTDGSFHCVCNAGFHVTRDGKNCEDMDECSIRNMCLNGMCINEDGSFKCICKPGFQLASDGRYCKDINECETPGICMNGRCVNTDGSYRCECFPGLAVGLDGRVCVDTHMRSTCYGGYKRGQCVKPLFGAVTKSECCCASTEYAFGEPCQPCPSQNSAEYQALCSSGPGITSAGSDINECALDPDICPNGICENLRGTYKCICNSGYEVDSTGKNCVDINECVLNSLLCDNGQCRNTPGSFVCTCPKGFIYKPELKTCEDIDECESSPCINGVCKNSPGSFICECSSESTLDPTKTICIETIKGTCWQTVIDGRCEININGATLKSQCCSSLGAAWGSPCTPCQVDPICGKGYSRIKGTQCEDIDECEVFPGVCKNGLCVNSKGSFKCQCPSGMTLDATGRICLDIRLETCFLRYEDEECTLPVAGRHRMDACCCSVGAAWGTEECEECPVRNTPEYEELCPRGPGFATKEITNGKRFFKDINECKMIPNLCTHGKCRNTIGSFKCRCDSGFALDSEERNCTDIDECRISPDLCGRGQCVNTPGDFECKCDEGYESGFMMMKNCMDIDECQRDPLLCRGGVCLNTEGSYRCECPPGHQLAPNISACIDINECELSAHLCPHGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETFCTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLCYDGFMASEDMKTCVDVNECDLNPNICLSGTCENTKGSFICHCDMGYSGKKGKTGCTDINECEIGAHNCDRHAVCTNTAGSFKCSCSPGWIGDGIKCTDLDECSNGTHMCSQHADCKNTMGSYRCLCKEGYTGDGFTCTDLDECSENLNLCGNGQCLNAPGGYRCECDMGFVPSADGKACEDIDECSLPNICVFGTCHNLPGLFRCECEIGYELDRSGGNCTDVNECLDPTTCISGNCVNTPGSYTCDCPPDFELNPTRVGCVDTRSGNCYLDIRPRGDNGDTACSNEIGVGVSKASCCCSLGKAWGTPCELCPPVNTSEYKILCPGGEGFRPNPITVILEDIDECQELPGLCQGGKCINTFGSFQCRCPTGYYLNEDTRVCDDVNECETPGICGPGTCYNTVGNYTCICPPDYMQVNGGNNCMDMRRSLCYRNYYADNQTCDGELLFNMTKKMCCCSYNIGRAWNKPCEQCPIPSTDEFATLCGSQRPGFVIDIYTGLPVDIDECREIPGVCENGVCINMVGSFRCECPVGFFYNDKLLVCEDIDECQNGPVCQRNAECINTAGSYRCDCKPGYRFTSTGQCNDRNECQEIPNICSHGQCIDTVGSFYCLCHTGFKTNADQTMCLDINECERDACGNGTCRNTIGSFNCRCNHGFILSHNNDCIDVDECATGNGNLCRNGQCINTVGSFQCQCNEGYEVAPDGRTCVDINECLLDPRKCAPGTCQNLDGSYRCICPPGYSLQNDKCEDIDECVEEPEICALGTCSNTEGSFKCLCPDGFSLSSTGRRCQDLRMSYCYAKFEGGKCSSPKSRNHSKQECCCALKGEGWGDPCELCPTEPDEAFRQICPYGSGIIVGPDDSAVDMDECKEPDVCKHGQCINTDGSYRCECPFGYILQGNECVDTDECSVGNPCGNGTCKNVIGGFECTCEEGFEPGPMMTCEDINECAQNPLLCAFRCVNTYGSYECKCPAGYVLREDRRMCKDEDECEEGKHDCAEKQMECKNLIGTYLCICGPGYQRRPDGEGCVDENECQTKPGICENGRCLNTRGSYTCECNDGFTASPNQDECLDNREGYCFTEVLQNMCQIGSSNRNPVTKSECCCDGGRGWGPHCEICPFQGTVAFKKLCPHGRGFMTNGADIDECKVIHDVCRNGECVNDRGSYHCICKTGYTPDITGTACVDLNECNQAPKPCNFICKNTEGSYQCSCPKGYILQEDGRSCKDLDECATKQHNCQFLCVNTIGSFTCKCPPGFTQHHTACIDNNECTSDINLCGSKGICQNTPGSFTCECQRGFSLDPSGASCEDVDECEGNHRCQHGCQNIIGGYRCSCPQGYLQHYQWNQCVDENECLSAHICGGASCHNTLGSYKCMCPAGFQYEQFSGGCQDINECGSAQAPCSYGCSNTEGGYLCACPPGYFRIGQGHCVSGMGMGRGNPEPPASGEMDDNSLSPEACYECKINGYPKRGRKRRSANETDASNIEDQPEIEANVSLASWDVEKTAVFAFNISHISNKVRILELLPALTTLTNHNRYLIESGNENGFFKINQKEGISYLHFTKKKPVAGTYSLQISSTPLYKKKELNQLEDKYDKDYLSGELGDNLKMKIQILLH

Target ORF information:

RefSeq Version	XM_015473179.1
Organism	Bos taurus(cattle)
Definition	Bos taurus fibrillin 1 (FBN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473179.1

ORF Insert Sequence:

ATGCGGCGAG GGGGGCTGCT GGAGGTCGCC CTGGGATTTA CCGTGCTCTT AGCGTCCTAC 
ACGAGCCATG GGGCGGATAC CAATTTGGAG GCTGGGAACG TGAAGGAAAC CAGAGCCAAC 
CGGGCCAAGA GAAGAGGTGG CGGAGGACAC GACGCGCTTA AAGGACCCAA TGTCTGTGGA 
TCACGTTATA ATGCTTACTG TTGCCCTGGA TGGAAGACTT TACCTGGTGG AAATCAGTGT 
ATTGTCCCCA TTTGCCGGCA TTCCTGTGGG GATGGATTTT GTTCGAGGCC AAATATGTGC 
ACTTGCCCAT CTGGTCAGAT AGCTCCTTCC TGTGGCTCCA GATCCATCCA ACATTGTAAC 
ATTCGCTGCA TGAATGGAGG TAGCTGCAGT GATGATCATT GTCTGTGCCA GAAAGGATAC 
ATAGGGACTC ACTGTGGACA ACCCGTCTGT GAAAGTGGCT GTCTTAACGG AGGCAGGTGC 
GTGGCCCCGA ATCGATGTGC GTGCACTTAC GGCTTTACTG GACCCCAGTG TGAAAGAGAT 
TACAGGACAG GTCCATGTTT TACTGTGATC AGCAACCAGA TGTGCCAGGG ACAGCTCAGC 
GGGATTGTCT GCACGAAAAC GCTCTGCTGT GCCACGGTCG GCCGTGCCTG GGGCCACCCC 
TGTGAGATGT GTCCCGCCCA GCCCCACCCC TGTCGCCGCG GCTTCATTCC AAATATCCGC 
ACAGGAGCTT GTCAAGATGT GGACGAATGC CAGGCCATCC CTGGGCTCTG TCAAGGAGGA 
AATTGCATTA ATACTGTTGG GTCTTTTGAG TGCAAATGCC CTGCTGGACA CAAATTTAAT 
GAAGTGTCAC AAAAATGTGA AGATATTGAC GAATGTAGCA CTATTCCTGG AATCTGTGAT 
GGGGGCGAAT GTACGAACAC AGTCAGCAGT TACTTTTGCA AATGTCCCCC TGGTTTTTAC 
ACCTCTCCAG ACGGTACCAG ATGCATAGAT GTCCGCCCAG GATACTGTTA CACGGCTCTG 
GCAAATGGGC GCTGTTCTAA CCAGCTGCCA CAGTCCATCA CCAAGATGCA GTGCTGCTGT 
GACGTCGGCC GGTGCTGGTC TCCCGGGGTC ACGGTCGCCC CCGAGATGTG TCCCATCAGA 
GCAACTGAGG ATTTCAACAA GCTGTGCTCC GTCCCTATGG TAATTCCCGA GAGGCCAGGA 
TATCCTCCTC CACCCCTTGG CCCCGTTCCT CCCGTTCAGC CTGTCCCTCC TGGCTTTCCT 
CCTGGGCCTC AAATCATGAT CCCTCGACCA CCAGTGGAAT ATCCGTATCC ATCGCGGGAA 
CCACCAAGGG TGCTGCCAGT CAACGTCACT GATTACTGCC AGCTCTTTCG CTATCTCTGT 
CAAAATGGAC GCTGCATCCC AACCCCCGGG AGCTACCGGT GTGAGTGCAA CAAAGGATTC 
CAGCTGGACC TCCGTGGGGA GTGCATTGAT GTTGATGAAT GTGAGAAGAA CCCTTGTGCT 
GGTGGCGAGT GTATTAACAC GCAGGGCTCT TACACCTGTC AGTGCCGACC TGGTTATCAG 
AGCACACTCA CGCGGACAGA GTGCCGAGAC ATTGATGAAT GTTTACAGAA TGGCCGTATC 
TGTAATAATG GACGTTGTAT CAACACGGAT GGCAGTTTCC ATTGTGTGTG TAATGCAGGA 
TTTCACGTCA CACGAGATGG GAAGAACTGT GAAGATATGG ATGAATGCAG CATAAGGAAT 
ATGTGCCTTA ATGGAATGTG TATCAACGAA GATGGCAGTT TCAAATGTAT TTGCAAACCT 
GGATTTCAGC TGGCATCAGA TGGGCGTTAT TGTAAAGATA TTAATGAGTG TGAAACACCT 
GGGATCTGCA TGAATGGGCG TTGCGTCAAC ACTGATGGCT CCTACCGATG TGAATGCTTT 
CCTGGACTGG CCGTGGGTCT GGACGGACGT GTGTGTGTTG ACACACACAT GCGGAGCACA 
TGCTATGGTG GATACAAAAG AGGCCAGTGT GTCAAACCCT TGTTTGGTGC TGTTACCAAG 
TCTGAATGCT GTTGTGCCAG CACTGAGTAT GCTTTCGGGG AACCTTGCCA ACCATGTCCT 
TCACAGAATT CAGCGGAGTA TCAGGCGCTC TGCAGCAGTG GGCCAGGAAT AACGTCAGCA 
GGCAGCGATA TAAATGAATG TGCATTAGAT CCTGATATTT GCCCAAATGG AATTTGTGAA 
AATCTCCGTG GGACCTACAA ATGTATATGC AACTCAGGAT ATGAAGTGGA TTCAACTGGG 
AAAAACTGTG TTGATATTAA TGAATGTGTA TTGAATAGTC TGCTTTGTGA CAATGGACAA 
TGTAGAAATA CTCCTGGAAG TTTTGTCTGT ACCTGCCCCA AGGGATTTAT ATACAAACCT 
GAACTGAAAA CCTGTGAAGA CATCGATGAA TGTGAATCAA GTCCTTGCAT TAATGGAGTC 
TGCAAGAACA GTCCGGGCTC TTTCATTTGT GAATGTTCTT CTGAAAGTAC TTTGGATCCA 
ACAAAAACCA TCTGCATAGA AACCATTAAG GGCACTTGTT GGCAGACTGT CATCGATGGG 
CGGTGTGAGA TCAACATCAA TGGAGCCACA TTGAAGTCCC AGTGCTGTTC ATCCCTTGGT 
GCTGCCTGGG GGAGCCCATG CACCCCATGC CAAGTCGATC CCATATGTGG GAAAGGCTAT 
TCAAGAATTA AAGGAACACA GTGTGAAGAT ATAGATGAAT GTGAAGTGTT CCCAGGAGTC 
TGCAAGAATG GCTTGTGTGT TAACTCCAAG GGATCATTTA AATGTCAGTG TCCCAGTGGG 
ATGACTTTGG ATGCCACAGG GAGGATTTGC CTAGATATCC GCCTGGAAAC CTGCTTCCTG 
AGGTACGAGG ACGAGGAGTG CACCCTGCCC GTGGCTGGGC GCCACCGCAT GGACGCCTGC 
TGCTGCTCAG TCGGGGCAGC CTGGGGCACC GAGGAGTGTG AGGAGTGTCC CGTGAGAAAC 
ACACCTGAGT ACGAGGAGCT CTGTCCCAGA GGACCCGGAT TCGCCACAAA AGAAATTACA 
AATGGCAAAC GTTTTTTCAA AGACATCAAC GAGTGTAAGA TGATTCCCAA CCTGTGCACC 
CACGGCAAGT GCAGGAACAC CATTGGCAGC TTCAAGTGCA GGTGTGACAG CGGCTTTGCT 
CTGGATTCTG AAGAAAGGAA CTGCACAGAC ATCGACGAAT GCCGCATATC CCCTGACCTC 
TGTGGCCGAG GCCAGTGTGT GAACACCCCC GGGGACTTTG AGTGCAAGTG CGATGAAGGC 
TATGAAAGCG GATTCATGAT GATGAAGAAC TGCATGGATA TCGATGAGTG TCAGAGAGAT 
CCGCTGTTGT GCCGAGGAGG CGTGTGCCTG AACACTGAGG GAAGTTACCG CTGTGAATGT 
CCCCCTGGCC ATCAGCTGGC CCCCAACATC TCGGCGTGTA TAGACATCAA CGAGTGTGAA 
CTAAGTGCAC ACCTCTGCCC CCATGGCCGC TGCGTGAACC TCATAGGAAA GTACCAGTGT 
GCCTGCAACC CTGGCTACCA CTCTACCCCT GACAGGCTGT TTTGTGTTGA CATTGATGAA 
TGCAGCATAA TGAACGGTGG CTGTGAGACC TTCTGCACAA ACTCTGAAGG TAGCTACGAA 
TGTAGCTGTC AGCCAGGATT TGCACTGATG CCTGACCAGA GGTCCTGCAC AGACATTGAT 
GAGTGTGAAG ATAACCCCAA TATCTGTGAT GGTGGTCAGT GTACAAACAT ACCTGGAGAG 
TACAGGTGCT TGTGTTATGA TGGATTCATG GCATCTGAAG ACATGAAGAC TTGCGTAGAT 
GTCAATGAAT GTGATCTGAA TCCAAATATC TGCCTAAGTG GAACCTGTGA AAACACCAAA 
GGCTCATTTA TCTGCCACTG TGATATGGGC TATTCAGGCA AAAAAGGAAA AACAGGCTGT 
ACAGACATCA ATGAATGTGA AATTGGAGCA CACAACTGCG ACAGACATGC TGTATGTACC 
AACACAGCAG GAAGCTTCAA ATGCAGCTGT AGCCCCGGGT GGATTGGAGA TGGCATTAAG 
TGCACTGACC TGGACGAATG CTCCAATGGA ACCCACATGT GCAGCCAACA TGCAGACTGC 
AAGAACACCA TGGGCTCCTA CCGCTGCCTC TGCAAAGAGG GGTACACAGG CGATGGCTTC 
ACTTGTACAG ATCTTGATGA GTGCTCTGAG AATCTAAACC TGTGTGGCAA CGGCCAGTGC 
CTTAACGCCC CAGGAGGATA CCGCTGTGAA TGTGACATGG GCTTTGTGCC CAGTGCTGAT 
GGGAAAGCCT GTGAAGATAT CGATGAGTGC TCTCTTCCGA ACATCTGTGT TTTTGGAACT 
TGCCACAACC TCCCTGGCCT CTTCCGCTGT GAATGTGAGA TCGGCTATGA ACTGGACAGA 
AGTGGTGGGA ACTGCACAGA CGTTAATGAA TGCCTGGATC CAACCACGTG CATCAGTGGG 
AACTGTGTCA ATACTCCAGG CAGCTATACC TGTGACTGTC CACCCGATTT TGAGCTGAAT 
CCAACTCGAG TTGGCTGTGT CGATACCCGC TCTGGAAACT GCTATTTGGA TATCCGTCCT 
CGGGGAGACA ATGGAGATAC AGCCTGCAGC AATGAAATTG GGGTTGGAGT GTCCAAGGCT 
TCCTGCTGCT GCTCTCTGGG TAAAGCCTGG GGCACTCCTT GTGAGCTGTG CCCGCCTGTG 
AACACATCTG AGTACAAAAT TCTTTGTCCT GGAGGAGAAG GTTTTCGACC CAACCCTATC 
ACCGTTATAT TGGAAGATAT TGATGAGTGC CAGGAGCTTC CAGGGTTGTG CCAAGGCGGG 
AAATGTATCA ATACCTTCGG CAGCTTCCAG TGCCGCTGTC CAACGGGCTA CTACCTGAAT 
GAAGATACCC GAGTGTGTGA TGATGTGAAT GAATGTGAAA CTCCCGGAAT CTGTGGTCCA 
GGGACATGTT ACAACACTGT TGGCAACTAC ACCTGTATCT GCCCTCCAGA CTACATGCAA 
GTGAATGGGG GGAATAATTG CATGGATATG AGGAGGAGTC TGTGCTACAG GAACTATTAT 
GCTGACAACC AGACCTGCGA TGGAGAACTG TTGTTCAACA TGACCAAGAA GATGTGCTGT 
TGTTCCTACA ACATTGGCCG GGCCTGGAAC AAGCCCTGTG AACAGTGCCC CATTCCAAGC 
ACAGATGAAT TTGCTACACT CTGTGGAAGT CAACGGCCAG GCTTTGTCAT CGACATCTAT 
ACCGGTTTAC CTGTCGATAT CGACGAGTGT CGGGAGATCC CAGGGGTCTG TGAAAACGGT 
GTGTGTATCA ACATGGTCGG CAGCTTCCGT TGTGAATGTC CAGTGGGCTT CTTCTATAAT 
GACAAGTTGC TGGTTTGTGA AGACATTGAC GAGTGTCAGA ACGGCCCAGT GTGCCAGCGA 
AACGCAGAGT GCATCAACAC CGCGGGCAGC TACCGCTGTG ATTGCAAACC CGGCTACCGA 
TTCACCTCCA CGGGGCAGTG CAATGATCGC AATGAATGTC AAGAAATCCC CAATATATGC 
AGCCATGGAC AGTGTATTGA CACAGTTGGA AGCTTTTATT GCCTTTGCCA CACTGGTTTT 
AAAACAAACG CTGATCAAAC CATGTGTTTG GACATAAATG AGTGTGAAAG AGATGCCTGT 
GGGAATGGCA CTTGCCGCAA CACGATTGGT TCCTTCAACT GCCGCTGCAA TCACGGTTTC 
ATCCTTTCCC ACAACAATGA CTGCATAGAT GTTGATGAAT GTGCAACTGG AAATGGGAAC 
CTTTGCAGAA ATGGCCAGTG TATCAATACA GTGGGGTCCT TCCAGTGTCA GTGCAATGAA 
GGCTATGAGG TGGCTCCAGA TGGGAGAACC TGTGTGGATA TCAATGAATG TCTCCTAGAT 
CCCAGAAAAT GTGCCCCAGG CACCTGTCAA AACTTGGATG GGTCCTACAG ATGTATCTGT 
CCACCTGGAT ACAGTCTCCA GAATGACAAG TGTGAAGATA TTGATGAATG TGTTGAAGAG 
CCAGAAATCT GTGCCTTGGG CACGTGCAGT AACACTGAAG GCAGCTTCAA ATGTCTGTGT 
CCAGACGGGT TCTCCTTGTC CTCCACTGGA AGAAGGTGCC AAGATTTACG AATGAGCTAC 
TGTTACGCGA AGTTTGAAGG AGGAAAGTGT TCATCACCCA AATCCAGAAA TCACTCCAAA 
CAGGAATGCT GCTGTGCCTT GAAGGGAGAA GGTTGGGGAG ATCCCTGCGA GCTGTGCCCC 
ACTGAACCCG ATGAGGCCTT CCGCCAGATC TGTCCCTATG GAAGTGGCAT CATCGTGGGA 
CCTGATGATT CAGCAGTTGA CATGGATGAG TGCAAAGAAC CCGATGTCTG TAAACACGGG 
CAGTGCATCA ACACCGATGG GTCCTATCGC TGCGAATGTC CCTTTGGCTA CATTCTGCAA 
GGGAATGAAT GTGTGGACAC TGATGAATGT TCGGTAGGCA ATCCTTGTGG AAATGGAACC 
TGCAAGAATG TGATTGGAGG TTTCGAATGC ACCTGTGAGG AGGGATTTGA GCCTGGGCCA 
ATGATGACTT GTGAAGACAT AAATGAGTGT GCCCAGAATC CTCTGCTCTG TGCCTTCCGC 
TGTGTAAATA CTTATGGGTC ATATGAATGC AAGTGTCCTG CTGGATATGT TCTGAGAGAA 
GACAGAAGGA TGTGCAAAGA TGAGGATGAG TGTGAAGAGG GAAAACACGA CTGTGCTGAA 
AAACAAATGG AGTGCAAGAA CCTCATTGGC ACATACCTCT GCATCTGTGG ACCTGGGTAT 
CAGCGAAGAC CTGACGGAGA AGGCTGCGTA GATGAAAATG AATGTCAGAC CAAGCCTGGG 
ATCTGTGAGA ACGGGCGCTG CCTCAACACC AGGGGGAGCT ATACCTGCGA GTGCAATGAC 
GGCTTTACAG CCAGCCCCAA CCAGGATGAG TGCCTCGACA ACCGAGAAGG GTACTGCTTC 
ACGGAGGTGC TCCAGAACAT GTGTCAGATC GGCTCAAGCA ACAGGAACCC CGTCACTAAG 
TCTGAATGCT GCTGCGATGG AGGGAGAGGC TGGGGGCCCC ACTGTGAGAT CTGCCCTTTC 
CAGGGCACTG TGGCTTTCAA AAAACTCTGC CCCCATGGCC GAGGGTTTAT GACCAATGGA 
GCAGATATTG ATGAGTGCAA GGTTATTCAT GATGTTTGCC GAAATGGGGA ATGTGTCAAT 
GACAGAGGAT CATATCATTG CATTTGTAAA ACTGGCTACA CTCCAGATAT AACTGGAACT 
GCCTGTGTAG ATCTAAATGA GTGCAACCAG GCTCCCAAAC CCTGCAATTT CATCTGTAAG 
AACACAGAAG GGAGTTACCA GTGTTCATGC CCGAAAGGCT ACATTCTGCA GGAGGACGGA 
AGGAGCTGCA AAGATCTTGA TGAGTGTGCA ACTAAGCAGC ACAACTGCCA GTTCCTGTGT 
GTTAACACCA TTGGCAGCTT CACATGTAAA TGTCCTCCTG GATTTACCCA ACACCATACT 
GCCTGCATTG ATAACAATGA ATGCACCTCT GACATCAACC TGTGTGGGTC AAAGGGCATT 
TGCCAGAACA CTCCTGGAAG CTTCACTTGT GAATGCCAGC GGGGATTCTC ACTTGATCCG 
AGCGGCGCAA GCTGTGAAGA TGTGGACGAG TGTGAGGGAA ACCATCGCTG CCAGCACGGC 
TGCCAGAACA TCATCGGCGG CTACAGGTGC AGCTGCCCCC AGGGCTATCT CCAGCACTAC 
CAGTGGAACC AGTGTGTTGA TGAAAATGAA TGCCTCAGTG CTCACATCTG TGGAGGAGCC 
TCCTGTCACA ACACCCTGGG GAGCTACAAG TGCATGTGCC CTGCCGGCTT CCAATACGAA 
CAGTTCAGTG GAGGTTGCCA AGACATCAAC GAATGTGGCT CTGCTCAGGC CCCCTGCAGT 
TATGGCTGTT CCAATACTGA GGGCGGCTAC CTGTGTGCCT GTCCACCTGG CTACTTCCGA 
ATAGGCCAAG GGCACTGTGT TTCTGGAATG GGCATGGGCC GGGGAAACCC AGAGCCACCT 
GCCAGCGGTG AAATGGATGA CAACTCACTC TCCCCAGAGG CTTGTTATGA GTGTAAGATC 
AACGGCTACC CCAAACGCGG CAGGAAGCGG AGAAGCGCAA ATGAAACAGA TGCCTCCAAC 
ATTGAGGATC AACCTGAGAT AGAAGCCAAT GTGAGTCTTG CAAGTTGGGA TGTTGAGAAG 
ACAGCCGTGT TCGCTTTCAA TATTTCCCAC ATCAGTAACA AGGTCCGAAT CCTAGAACTC 
CTTCCAGCCC TCACGACTCT GACGAATCAC AACAGATACT TGATTGAATC TGGAAATGAA 
AACGGCTTCT TTAAAATCAA CCAAAAGGAA GGGATCAGCT ACCTCCACTT CACAAAGAAG 
AAGCCAGTGG CTGGAACCTA TTCATTACAA ATCAGTAGTA CTCCACTTTA TAAAAAGAAA 
GAACTTAATC AGCTAGAAGA TAAATATGAC AAAGACTACC TCAGTGGTGA ACTGGGTGAT 
AATCTGAAGA TGAAAATTCA GATTTTGCTT CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBN1 ( XM_015473179.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328665.1 Bos taurus fibrillin 1 (FBN1), transcript variant X1, mRNA.

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CloneID	OBa91701
Clone ID Related Accession (Same CDS sequence)	XM_015473179.1 , XM_015473179.2
Accession Version	XM_015473179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	fibrillin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGAG GGGGGCTGCT GGAGGTCGCC CTGGGATTTA CCGTGCTCTT AGCGTCCTAC 
ACGAGCCATG GGGCGGATAC CAATTTGGAG GCTGGGAACG TGAAGGAAAC CAGAGCCAAC 
CGGGCCAAGA GAAGAGGTGG CGGAGGACAC GACGCGCTTA AAGGACCCAA TGTCTGTGGA 
TCACGTTATA ATGCTTACTG TTGCCCTGGA TGGAAGACTT TACCTGGTGG AAATCAGTGT 
ATTGTCCCCA TTTGCCGGCA TTCCTGTGGG GATGGATTTT GTTCGAGGCC AAATATGTGC 
ACTTGCCCAT CTGGTCAGAT AGCTCCTTCC TGTGGCTCCA GATCCATCCA ACATTGTAAC 
ATTCGCTGCA TGAATGGAGG TAGCTGCAGT GATGATCATT GTCTGTGCCA GAAAGGATAC 
ATAGGGACTC ACTGTGGACA ACCCGTCTGT GAAAGTGGCT GTCTTAACGG AGGCAGGTGC 
GTGGCCCCGA ATCGATGTGC GTGCACTTAC GGCTTTACTG GACCCCAGTG TGAAAGAGAT 
TACAGGACAG GTCCATGTTT TACTGTGATC AGCAACCAGA TGTGCCAGGG ACAGCTCAGC 
GGGATTGTCT GCACGAAAAC GCTCTGCTGT GCCACGGTCG GCCGTGCCTG GGGCCACCCC 
TGTGAGATGT GTCCCGCCCA GCCCCACCCC TGTCGCCGCG GCTTCATTCC AAATATCCGC 
ACAGGAGCTT GTCAAGATGT GGACGAATGC CAGGCCATCC CTGGGCTCTG TCAAGGAGGA 
AATTGCATTA ATACTGTTGG GTCTTTTGAG TGCAAATGCC CTGCTGGACA CAAATTTAAT 
GAAGTGTCAC AAAAATGTGA AGATATTGAC GAATGTAGCA CTATTCCTGG AATCTGTGAT 
GGGGGCGAAT GTACGAACAC AGTCAGCAGT TACTTTTGCA AATGTCCCCC TGGTTTTTAC 
ACCTCTCCAG ACGGTACCAG ATGCATAGAT GTCCGCCCAG GATACTGTTA CACGGCTCTG 
GCAAATGGGC GCTGTTCTAA CCAGCTGCCA CAGTCCATCA CCAAGATGCA GTGCTGCTGT 
GACGTCGGCC GGTGCTGGTC TCCCGGGGTC ACGGTCGCCC CCGAGATGTG TCCCATCAGA 
GCAACTGAGG ATTTCAACAA GCTGTGCTCC GTCCCTATGG TAATTCCCGA GAGGCCAGGA 
TATCCTCCTC CACCCCTTGG CCCCGTTCCT CCCGTTCAGC CTGTCCCTCC TGGCTTTCCT 
CCTGGGCCTC AAATCATGAT CCCTCGACCA CCAGTGGAAT ATCCGTATCC ATCGCGGGAA 
CCACCAAGGG TGCTGCCAGT CAACGTCACT GATTACTGCC AGCTCTTTCG CTATCTCTGT 
CAAAATGGAC GCTGCATCCC AACCCCCGGG AGCTACCGGT GTGAGTGCAA CAAAGGATTC 
CAGCTGGACC TCCGTGGGGA GTGCATTGAT GTTGATGAAT GTGAGAAGAA CCCTTGTGCT 
GGTGGCGAGT GTATTAACAC GCAGGGCTCT TACACCTGTC AGTGCCGACC TGGTTATCAG 
AGCACACTCA CGCGGACAGA GTGCCGAGAC ATTGATGAAT GTTTACAGAA TGGCCGTATC 
TGTAATAATG GACGTTGTAT CAACACGGAT GGCAGTTTCC ATTGTGTGTG TAATGCAGGA 
TTTCACGTCA CACGAGATGG GAAGAACTGT GAAGATATGG ATGAATGCAG CATAAGGAAT 
ATGTGCCTTA ATGGAATGTG TATCAACGAA GATGGCAGTT TCAAATGTAT TTGCAAACCT 
GGATTTCAGC TGGCATCAGA TGGGCGTTAT TGTAAAGATA TTAATGAGTG TGAAACACCT 
GGGATCTGCA TGAATGGGCG TTGCGTCAAC ACTGATGGCT CCTACCGATG TGAATGCTTT 
CCTGGACTGG CCGTGGGTCT GGACGGACGT GTGTGTGTTG ACACACACAT GCGGAGCACA 
TGCTATGGTG GATACAAAAG AGGCCAGTGT GTCAAACCCT TGTTTGGTGC TGTTACCAAG 
TCTGAATGCT GTTGTGCCAG CACTGAGTAT GCTTTCGGGG AACCTTGCCA ACCATGTCCT 
TCACAGAATT CAGCGGAGTA TCAGGCGCTC TGCAGCAGTG GGCCAGGAAT AACGTCAGCA 
GGCAGCGATA TAAATGAATG TGCATTAGAT CCTGATATTT GCCCAAATGG AATTTGTGAA 
AATCTCCGTG GGACCTACAA ATGTATATGC AACTCAGGAT ATGAAGTGGA TTCAACTGGG 
AAAAACTGTG TTGATATTAA TGAATGTGTA TTGAATAGTC TGCTTTGTGA CAATGGACAA 
TGTAGAAATA CTCCTGGAAG TTTTGTCTGT ACCTGCCCCA AGGGATTTAT ATACAAACCT 
GAACTGAAAA CCTGTGAAGA CATCGATGAA TGTGAATCAA GTCCTTGCAT TAATGGAGTC 
TGCAAGAACA GTCCGGGCTC TTTCATTTGT GAATGTTCTT CTGAAAGTAC TTTGGATCCA 
ACAAAAACCA TCTGCATAGA AACCATTAAG GGCACTTGTT GGCAGACTGT CATCGATGGG 
CGGTGTGAGA TCAACATCAA TGGAGCCACA TTGAAGTCCC AGTGCTGTTC ATCCCTTGGT 
GCTGCCTGGG GGAGCCCATG CACCCCATGC CAAGTCGATC CCATATGTGG GAAAGGCTAT 
TCAAGAATTA AAGGAACACA GTGTGAAGAT ATAGATGAAT GTGAAGTGTT CCCAGGAGTC 
TGCAAGAATG GCTTGTGTGT TAACTCCAAG GGATCATTTA AATGTCAGTG TCCCAGTGGG 
ATGACTTTGG ATGCCACAGG GAGGATTTGC CTAGATATCC GCCTGGAAAC CTGCTTCCTG 
AGGTACGAGG ACGAGGAGTG CACCCTGCCC GTGGCTGGGC GCCACCGCAT GGACGCCTGC 
TGCTGCTCAG TCGGGGCAGC CTGGGGCACC GAGGAGTGTG AGGAGTGTCC CGTGAGAAAC 
ACACCTGAGT ACGAGGAGCT CTGTCCCAGA GGACCCGGAT TCGCCACAAA AGAAATTACA 
AATGGCAAAC GTTTTTTCAA AGACATCAAC GAGTGTAAGA TGATTCCCAA CCTGTGCACC 
CACGGCAAGT GCAGGAACAC CATTGGCAGC TTCAAGTGCA GGTGTGACAG CGGCTTTGCT 
CTGGATTCTG AAGAAAGGAA CTGCACAGAC ATCGACGAAT GCCGCATATC CCCTGACCTC 
TGTGGCCGAG GCCAGTGTGT GAACACCCCC GGGGACTTTG AGTGCAAGTG CGATGAAGGC 
TATGAAAGCG GATTCATGAT GATGAAGAAC TGCATGGATA TCGATGAGTG TCAGAGAGAT 
CCGCTGTTGT GCCGAGGAGG CGTGTGCCTG AACACTGAGG GAAGTTACCG CTGTGAATGT 
CCCCCTGGCC ATCAGCTGGC CCCCAACATC TCGGCGTGTA TAGACATCAA CGAGTGTGAA 
CTAAGTGCAC ACCTCTGCCC CCATGGCCGC TGCGTGAACC TCATAGGAAA GTACCAGTGT 
GCCTGCAACC CTGGCTACCA CTCTACCCCT GACAGGCTGT TTTGTGTTGA CATTGATGAA 
TGCAGCATAA TGAACGGTGG CTGTGAGACC TTCTGCACAA ACTCTGAAGG TAGCTACGAA 
TGTAGCTGTC AGCCAGGATT TGCACTGATG CCTGACCAGA GGTCCTGCAC AGACATTGAT 
GAGTGTGAAG ATAACCCCAA TATCTGTGAT GGTGGTCAGT GTACAAACAT ACCTGGAGAG 
TACAGGTGCT TGTGTTATGA TGGATTCATG GCATCTGAAG ACATGAAGAC TTGCGTAGAT 
GTCAATGAAT GTGATCTGAA TCCAAATATC TGCCTAAGTG GAACCTGTGA AAACACCAAA 
GGCTCATTTA TCTGCCACTG TGATATGGGC TATTCAGGCA AAAAAGGAAA AACAGGCTGT 
ACAGACATCA ATGAATGTGA AATTGGAGCA CACAACTGCG ACAGACATGC TGTATGTACC 
AACACAGCAG GAAGCTTCAA ATGCAGCTGT AGCCCCGGGT GGATTGGAGA TGGCATTAAG 
TGCACTGACC TGGACGAATG CTCCAATGGA ACCCACATGT GCAGCCAACA TGCAGACTGC 
AAGAACACCA TGGGCTCCTA CCGCTGCCTC TGCAAAGAGG GGTACACAGG CGATGGCTTC 
ACTTGTACAG ATCTTGATGA GTGCTCTGAG AATCTAAACC TGTGTGGCAA CGGCCAGTGC 
CTTAACGCCC CAGGAGGATA CCGCTGTGAA TGTGACATGG GCTTTGTGCC CAGTGCTGAT 
GGGAAAGCCT GTGAAGATAT CGATGAGTGC TCTCTTCCGA ACATCTGTGT TTTTGGAACT 
TGCCACAACC TCCCTGGCCT CTTCCGCTGT GAATGTGAGA TCGGCTATGA ACTGGACAGA 
AGTGGTGGGA ACTGCACAGA CGTTAATGAA TGCCTGGATC CAACCACGTG CATCAGTGGG 
AACTGTGTCA ATACTCCAGG CAGCTATACC TGTGACTGTC CACCCGATTT TGAGCTGAAT 
CCAACTCGAG TTGGCTGTGT CGATACCCGC TCTGGAAACT GCTATTTGGA TATCCGTCCT 
CGGGGAGACA ATGGAGATAC AGCCTGCAGC AATGAAATTG GGGTTGGAGT GTCCAAGGCT 
TCCTGCTGCT GCTCTCTGGG TAAAGCCTGG GGCACTCCTT GTGAGCTGTG CCCGCCTGTG 
AACACATCTG AGTACAAAAT TCTTTGTCCT GGAGGAGAAG GTTTTCGACC CAACCCTATC 
ACCGTTATAT TGGAAGATAT TGATGAGTGC CAGGAGCTTC CAGGGTTGTG CCAAGGCGGG 
AAATGTATCA ATACCTTCGG CAGCTTCCAG TGCCGCTGTC CAACGGGCTA CTACCTGAAT 
GAAGATACCC GAGTGTGTGA TGATGTGAAT GAATGTGAAA CTCCCGGAAT CTGTGGTCCA 
GGGACATGTT ACAACACTGT TGGCAACTAC ACCTGTATCT GCCCTCCAGA CTACATGCAA 
GTGAATGGGG GGAATAATTG CATGGATATG AGGAGGAGTC TGTGCTACAG GAACTATTAT 
GCTGACAACC AGACCTGCGA TGGAGAACTG TTGTTCAACA TGACCAAGAA GATGTGCTGT 
TGTTCCTACA ACATTGGCCG GGCCTGGAAC AAGCCCTGTG AACAGTGCCC CATTCCAAGC 
ACAGATGAAT TTGCTACACT CTGTGGAAGT CAACGGCCAG GCTTTGTCAT CGACATCTAT 
ACCGGTTTAC CTGTCGATAT CGACGAGTGT CGGGAGATCC CAGGGGTCTG TGAAAACGGT 
GTGTGTATCA ACATGGTCGG CAGCTTCCGT TGTGAATGTC CAGTGGGCTT CTTCTATAAT 
GACAAGTTGC TGGTTTGTGA AGACATTGAC GAGTGTCAGA ACGGCCCAGT GTGCCAGCGA 
AACGCAGAGT GCATCAACAC CGCGGGCAGC TACCGCTGTG ATTGCAAACC CGGCTACCGA 
TTCACCTCCA CGGGGCAGTG CAATGATCGC AATGAATGTC AAGAAATCCC CAATATATGC 
AGCCATGGAC AGTGTATTGA CACAGTTGGA AGCTTTTATT GCCTTTGCCA CACTGGTTTT 
AAAACAAACG CTGATCAAAC CATGTGTTTG GACATAAATG AGTGTGAAAG AGATGCCTGT 
GGGAATGGCA CTTGCCGCAA CACGATTGGT TCCTTCAACT GCCGCTGCAA TCACGGTTTC 
ATCCTTTCCC ACAACAATGA CTGCATAGAT GTTGATGAAT GTGCAACTGG AAATGGGAAC 
CTTTGCAGAA ATGGCCAGTG TATCAATACA GTGGGGTCCT TCCAGTGTCA GTGCAATGAA 
GGCTATGAGG TGGCTCCAGA TGGGAGAACC TGTGTGGATA TCAATGAATG TCTCCTAGAT 
CCCAGAAAAT GTGCCCCAGG CACCTGTCAA AACTTGGATG GGTCCTACAG ATGTATCTGT 
CCACCTGGAT ACAGTCTCCA GAATGACAAG TGTGAAGATA TTGATGAATG TGTTGAAGAG 
CCAGAAATCT GTGCCTTGGG CACGTGCAGT AACACTGAAG GCAGCTTCAA ATGTCTGTGT 
CCAGACGGGT TCTCCTTGTC CTCCACTGGA AGAAGGTGCC AAGATTTACG AATGAGCTAC 
TGTTACGCGA AGTTTGAAGG AGGAAAGTGT TCATCACCCA AATCCAGAAA TCACTCCAAA 
CAGGAATGCT GCTGTGCCTT GAAGGGAGAA GGTTGGGGAG ATCCCTGCGA GCTGTGCCCC 
ACTGAACCCG ATGAGGCCTT CCGCCAGATC TGTCCCTATG GAAGTGGCAT CATCGTGGGA 
CCTGATGATT CAGCAGTTGA CATGGATGAG TGCAAAGAAC CCGATGTCTG TAAACACGGG 
CAGTGCATCA ACACCGATGG GTCCTATCGC TGCGAATGTC CCTTTGGCTA CATTCTGCAA 
GGGAATGAAT GTGTGGACAC TGATGAATGT TCGGTAGGCA ATCCTTGTGG AAATGGAACC 
TGCAAGAATG TGATTGGAGG TTTCGAATGC ACCTGTGAGG AGGGATTTGA GCCTGGGCCA 
ATGATGACTT GTGAAGACAT AAATGAGTGT GCCCAGAATC CTCTGCTCTG TGCCTTCCGC 
TGTGTAAATA CTTATGGGTC ATATGAATGC AAGTGTCCTG CTGGATATGT TCTGAGAGAA 
GACAGAAGGA TGTGCAAAGA TGAGGATGAG TGTGAAGAGG GAAAACACGA CTGTGCTGAA 
AAACAAATGG AGTGCAAGAA CCTCATTGGC ACATACCTCT GCATCTGTGG ACCTGGGTAT 
CAGCGAAGAC CTGACGGAGA AGGCTGCGTA GATGAAAATG AATGTCAGAC CAAGCCTGGG 
ATCTGTGAGA ACGGGCGCTG CCTCAACACC AGGGGGAGCT ATACCTGCGA GTGCAATGAC 
GGCTTTACAG CCAGCCCCAA CCAGGATGAG TGCCTCGACA ACCGAGAAGG GTACTGCTTC 
ACGGAGGTGC TCCAGAACAT GTGTCAGATC GGCTCAAGCA ACAGGAACCC CGTCACTAAG 
TCTGAATGCT GCTGCGATGG AGGGAGAGGC TGGGGGCCCC ACTGTGAGAT CTGCCCTTTC 
CAGGGCACTG TGGCTTTCAA AAAACTCTGC CCCCATGGCC GAGGGTTTAT GACCAATGGA 
GCAGATATTG ATGAGTGCAA GGTTATTCAT GATGTTTGCC GAAATGGGGA ATGTGTCAAT 
GACAGAGGAT CATATCATTG CATTTGTAAA ACTGGCTACA CTCCAGATAT AACTGGAACT 
GCCTGTGTAG ATCTAAATGA GTGCAACCAG GCTCCCAAAC CCTGCAATTT CATCTGTAAG 
AACACAGAAG GGAGTTACCA GTGTTCATGC CCGAAAGGCT ACATTCTGCA GGAGGACGGA 
AGGAGCTGCA AAGATCTTGA TGAGTGTGCA ACTAAGCAGC ACAACTGCCA GTTCCTGTGT 
GTTAACACCA TTGGCAGCTT CACATGTAAA TGTCCTCCTG GATTTACCCA ACACCATACT 
GCCTGCATTG ATAACAATGA ATGCACCTCT GACATCAACC TGTGTGGGTC AAAGGGCATT 
TGCCAGAACA CTCCTGGAAG CTTCACTTGT GAATGCCAGC GGGGATTCTC ACTTGATCCG 
AGCGGCGCAA GCTGTGAAGA TGTGGACGAG TGTGAGGGAA ACCATCGCTG CCAGCACGGC 
TGCCAGAACA TCATCGGCGG CTACAGGTGC AGCTGCCCCC AGGGCTATCT CCAGCACTAC 
CAGTGGAACC AGTGTGTTGA TGAAAATGAA TGCCTCAGTG CTCACATCTG TGGAGGAGCC 
TCCTGTCACA ACACCCTGGG GAGCTACAAG TGCATGTGCC CTGCCGGCTT CCAATACGAA 
CAGTTCAGTG GAGGTTGCCA AGACATCAAC GAATGTGGCT CTGCTCAGGC CCCCTGCAGT 
TATGGCTGTT CCAATACTGA GGGCGGCTAC CTGTGTGCCT GTCCACCTGG CTACTTCCGA 
ATAGGCCAAG GGCACTGTGT TTCTGGAATG GGCATGGGCC GGGGAAACCC AGAGCCACCT 
GCCAGCGGTG AAATGGATGA CAACTCACTC TCCCCAGAGG CTTGTTATGA GTGTAAGATC 
AACGGCTACC CCAAACGCGG CAGGAAGCGG AGAAGCGCAA ATGAAACAGA TGCCTCCAAC 
ATTGAGGATC AACCTGAGAT AGAAGCCAAT GTGAGTCTTG CAAGTTGGGA TGTTGAGAAG 
ACAGCCGTGT TCGCTTTCAA TATTTCCCAC ATCAGTAACA AGGTCCGAAT CCTAGAACTC 
CTTCCAGCCC TCACGACTCT GACGAATCAC AACAGATACT TGATTGAATC TGGAAATGAA 
AACGGCTTCT TTAAAATCAA CCAAAAGGAA GGGATCAGCT ACCTCCACTT CACAAAGAAG 
AAGCCAGTGG CTGGAACCTA TTCATTACAA ATCAGTAGTA CTCCACTTTA TAAAAAGAAA 
GAACTTAATC AGCTAGAAGA TAAATATGAC AAAGACTACC TCAGTGGTGA ACTGGGTGAT 
AATCTGAAGA TGAAAATTCA GATTTTGCTT CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328665.1
CDS	336..8951
Translation	MRRGGLLEVALGFTVLLASYTSHGADTNLEAGNVKETRANRAKRRGGGGHDALKGPNVCGSRYNAYCCPGWKTLPGGNQCIVPICRHSCGDGFCSRPNMCTCPSGQIAPSCGSRSIQHCNIRCMNGGSCSDDHCLCQKGYIGTHCGQPVCESGCLNGGRCVAPNRCACTYGFTGPQCERDYRTGPCFTVISNQMCQGQLSGIVCTKTLCCATVGRAWGHPCEMCPAQPHPCRRGFIPNIRTGACQDVDECQAIPGLCQGGNCINTVGSFECKCPAGHKFNEVSQKCEDIDECSTIPGICDGGECTNTVSSYFCKCPPGFYTSPDGTRCIDVRPGYCYTALANGRCSNQLPQSITKMQCCCDVGRCWSPGVTVAPEMCPIRATEDFNKLCSVPMVIPERPGYPPPPLGPVPPVQPVPPGFPPGPQIMIPRPPVEYPYPSREPPRVLPVNVTDYCQLFRYLCQNGRCIPTPGSYRCECNKGFQLDLRGECIDVDECEKNPCAGGECINTQGSYTCQCRPGYQSTLTRTECRDIDECLQNGRICNNGRCINTDGSFHCVCNAGFHVTRDGKNCEDMDECSIRNMCLNGMCINEDGSFKCICKPGFQLASDGRYCKDINECETPGICMNGRCVNTDGSYRCECFPGLAVGLDGRVCVDTHMRSTCYGGYKRGQCVKPLFGAVTKSECCCASTEYAFGEPCQPCPSQNSAEYQALCSSGPGITSAGSDINECALDPDICPNGICENLRGTYKCICNSGYEVDSTGKNCVDINECVLNSLLCDNGQCRNTPGSFVCTCPKGFIYKPELKTCEDIDECESSPCINGVCKNSPGSFICECSSESTLDPTKTICIETIKGTCWQTVIDGRCEININGATLKSQCCSSLGAAWGSPCTPCQVDPICGKGYSRIKGTQCEDIDECEVFPGVCKNGLCVNSKGSFKCQCPSGMTLDATGRICLDIRLETCFLRYEDEECTLPVAGRHRMDACCCSVGAAWGTEECEECPVRNTPEYEELCPRGPGFATKEITNGKRFFKDINECKMIPNLCTHGKCRNTIGSFKCRCDSGFALDSEERNCTDIDECRISPDLCGRGQCVNTPGDFECKCDEGYESGFMMMKNCMDIDECQRDPLLCRGGVCLNTEGSYRCECPPGHQLAPNISACIDINECELSAHLCPHGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETFCTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLCYDGFMASEDMKTCVDVNECDLNPNICLSGTCENTKGSFICHCDMGYSGKKGKTGCTDINECEIGAHNCDRHAVCTNTAGSFKCSCSPGWIGDGIKCTDLDECSNGTHMCSQHADCKNTMGSYRCLCKEGYTGDGFTCTDLDECSENLNLCGNGQCLNAPGGYRCECDMGFVPSADGKACEDIDECSLPNICVFGTCHNLPGLFRCECEIGYELDRSGGNCTDVNECLDPTTCISGNCVNTPGSYTCDCPPDFELNPTRVGCVDTRSGNCYLDIRPRGDNGDTACSNEIGVGVSKASCCCSLGKAWGTPCELCPPVNTSEYKILCPGGEGFRPNPITVILEDIDECQELPGLCQGGKCINTFGSFQCRCPTGYYLNEDTRVCDDVNECETPGICGPGTCYNTVGNYTCICPPDYMQVNGGNNCMDMRRSLCYRNYYADNQTCDGELLFNMTKKMCCCSYNIGRAWNKPCEQCPIPSTDEFATLCGSQRPGFVIDIYTGLPVDIDECREIPGVCENGVCINMVGSFRCECPVGFFYNDKLLVCEDIDECQNGPVCQRNAECINTAGSYRCDCKPGYRFTSTGQCNDRNECQEIPNICSHGQCIDTVGSFYCLCHTGFKTNADQTMCLDINECERDACGNGTCRNTIGSFNCRCNHGFILSHNNDCIDVDECATGNGNLCRNGQCINTVGSFQCQCNEGYEVAPDGRTCVDINECLLDPRKCAPGTCQNLDGSYRCICPPGYSLQNDKCEDIDECVEEPEICALGTCSNTEGSFKCLCPDGFSLSSTGRRCQDLRMSYCYAKFEGGKCSSPKSRNHSKQECCCALKGEGWGDPCELCPTEPDEAFRQICPYGSGIIVGPDDSAVDMDECKEPDVCKHGQCINTDGSYRCECPFGYILQGNECVDTDECSVGNPCGNGTCKNVIGGFECTCEEGFEPGPMMTCEDINECAQNPLLCAFRCVNTYGSYECKCPAGYVLREDRRMCKDEDECEEGKHDCAEKQMECKNLIGTYLCICGPGYQRRPDGEGCVDENECQTKPGICENGRCLNTRGSYTCECNDGFTASPNQDECLDNREGYCFTEVLQNMCQIGSSNRNPVTKSECCCDGGRGWGPHCEICPFQGTVAFKKLCPHGRGFMTNGADIDECKVIHDVCRNGECVNDRGSYHCICKTGYTPDITGTACVDLNECNQAPKPCNFICKNTEGSYQCSCPKGYILQEDGRSCKDLDECATKQHNCQFLCVNTIGSFTCKCPPGFTQHHTACIDNNECTSDINLCGSKGICQNTPGSFTCECQRGFSLDPSGASCEDVDECEGNHRCQHGCQNIIGGYRCSCPQGYLQHYQWNQCVDENECLSAHICGGASCHNTLGSYKCMCPAGFQYEQFSGGCQDINECGSAQAPCSYGCSNTEGGYLCACPPGYFRIGQGHCVSGMGMGRGNPEPPASGEMDDNSLSPEACYECKINGYPKRGRKRRSANETDASNIEDQPEIEANVSLASWDVEKTAVFAFNISHISNKVRILELLPALTTLTNHNRYLIESGNENGFFKINQKEGISYLHFTKKKPVAGTYSLQISSTPLYKKKELNQLEDKYDKDYLSGELGDNLKMKIQILLH

Target ORF information:

RefSeq Version	XM_015473179.2
Organism	Bos taurus(cattle)
Definition	Bos taurus fibrillin 1 (FBN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473179.2

ORF Insert Sequence:

ATGCGGCGAG GGGGGCTGCT GGAGGTCGCC CTGGGATTTA CCGTGCTCTT AGCGTCCTAC 
ACGAGCCATG GGGCGGATAC CAATTTGGAG GCTGGGAACG TGAAGGAAAC CAGAGCCAAC 
CGGGCCAAGA GAAGAGGTGG CGGAGGACAC GACGCGCTTA AAGGACCCAA TGTCTGTGGA 
TCACGTTATA ATGCTTACTG TTGCCCTGGA TGGAAGACTT TACCTGGTGG AAATCAGTGT 
ATTGTCCCCA TTTGCCGGCA TTCCTGTGGG GATGGATTTT GTTCGAGGCC AAATATGTGC 
ACTTGCCCAT CTGGTCAGAT AGCTCCTTCC TGTGGCTCCA GATCCATCCA ACATTGTAAC 
ATTCGCTGCA TGAATGGAGG TAGCTGCAGT GATGATCATT GTCTGTGCCA GAAAGGATAC 
ATAGGGACTC ACTGTGGACA ACCCGTCTGT GAAAGTGGCT GTCTTAACGG AGGCAGGTGC 
GTGGCCCCGA ATCGATGTGC GTGCACTTAC GGCTTTACTG GACCCCAGTG TGAAAGAGAT 
TACAGGACAG GTCCATGTTT TACTGTGATC AGCAACCAGA TGTGCCAGGG ACAGCTCAGC 
GGGATTGTCT GCACGAAAAC GCTCTGCTGT GCCACGGTCG GCCGTGCCTG GGGCCACCCC 
TGTGAGATGT GTCCCGCCCA GCCCCACCCC TGTCGCCGCG GCTTCATTCC AAATATCCGC 
ACAGGAGCTT GTCAAGATGT GGACGAATGC CAGGCCATCC CTGGGCTCTG TCAAGGAGGA 
AATTGCATTA ATACTGTTGG GTCTTTTGAG TGCAAATGCC CTGCTGGACA CAAATTTAAT 
GAAGTGTCAC AAAAATGTGA AGATATTGAC GAATGTAGCA CTATTCCTGG AATCTGTGAT 
GGGGGCGAAT GTACGAACAC AGTCAGCAGT TACTTTTGCA AATGTCCCCC TGGTTTTTAC 
ACCTCTCCAG ACGGTACCAG ATGCATAGAT GTCCGCCCAG GATACTGTTA CACGGCTCTG 
GCAAATGGGC GCTGTTCTAA CCAGCTGCCA CAGTCCATCA CCAAGATGCA GTGCTGCTGT 
GACGTCGGCC GGTGCTGGTC TCCCGGGGTC ACGGTCGCCC CCGAGATGTG TCCCATCAGA 
GCAACTGAGG ATTTCAACAA GCTGTGCTCC GTCCCTATGG TAATTCCCGA GAGGCCAGGA 
TATCCTCCTC CACCCCTTGG CCCCGTTCCT CCCGTTCAGC CTGTCCCTCC TGGCTTTCCT 
CCTGGGCCTC AAATCATGAT CCCTCGACCA CCAGTGGAAT ATCCGTATCC ATCGCGGGAA 
CCACCAAGGG TGCTGCCAGT CAACGTCACT GATTACTGCC AGCTCTTTCG CTATCTCTGT 
CAAAATGGAC GCTGCATCCC AACCCCCGGG AGCTACCGGT GTGAGTGCAA CAAAGGATTC 
CAGCTGGACC TCCGTGGGGA GTGCATTGAT GTTGATGAAT GTGAGAAGAA CCCTTGTGCT 
GGTGGCGAGT GTATTAACAC GCAGGGCTCT TACACCTGTC AGTGCCGACC TGGTTATCAG 
AGCACACTCA CGCGGACAGA GTGCCGAGAC ATTGATGAAT GTTTACAGAA TGGCCGTATC 
TGTAATAATG GACGTTGTAT CAACACGGAT GGCAGTTTCC ATTGTGTGTG TAATGCAGGA 
TTTCACGTCA CACGAGATGG GAAGAACTGT GAAGATATGG ATGAATGCAG CATAAGGAAT 
ATGTGCCTTA ATGGAATGTG TATCAACGAA GATGGCAGTT TCAAATGTAT TTGCAAACCT 
GGATTTCAGC TGGCATCAGA TGGGCGTTAT TGTAAAGATA TTAATGAGTG TGAAACACCT 
GGGATCTGCA TGAATGGGCG TTGCGTCAAC ACTGATGGCT CCTACCGATG TGAATGCTTT 
CCTGGACTGG CCGTGGGTCT GGACGGACGT GTGTGTGTTG ACACACACAT GCGGAGCACA 
TGCTATGGTG GATACAAAAG AGGCCAGTGT GTCAAACCCT TGTTTGGTGC TGTTACCAAG 
TCTGAATGCT GTTGTGCCAG CACTGAGTAT GCTTTCGGGG AACCTTGCCA ACCATGTCCT 
TCACAGAATT CAGCGGAGTA TCAGGCGCTC TGCAGCAGTG GGCCAGGAAT AACGTCAGCA 
GGCAGCGATA TAAATGAATG TGCATTAGAT CCTGATATTT GCCCAAATGG AATTTGTGAA 
AATCTCCGTG GGACCTACAA ATGTATATGC AACTCAGGAT ATGAAGTGGA TTCAACTGGG 
AAAAACTGTG TTGATATTAA TGAATGTGTA TTGAATAGTC TGCTTTGTGA CAATGGACAA 
TGTAGAAATA CTCCTGGAAG TTTTGTCTGT ACCTGCCCCA AGGGATTTAT ATACAAACCT 
GAACTGAAAA CCTGTGAAGA CATCGATGAA TGTGAATCAA GTCCTTGCAT TAATGGAGTC 
TGCAAGAACA GTCCGGGCTC TTTCATTTGT GAATGTTCTT CTGAAAGTAC TTTGGATCCA 
ACAAAAACCA TCTGCATAGA AACCATTAAG GGCACTTGTT GGCAGACTGT CATCGATGGG 
CGGTGTGAGA TCAACATCAA TGGAGCCACA TTGAAGTCCC AGTGCTGTTC ATCCCTTGGT 
GCTGCCTGGG GGAGCCCATG CACCCCATGC CAAGTCGATC CCATATGTGG GAAAGGCTAT 
TCAAGAATTA AAGGAACACA GTGTGAAGAT ATAGATGAAT GTGAAGTGTT CCCAGGAGTC 
TGCAAGAATG GCTTGTGTGT TAACTCCAAG GGATCATTTA AATGTCAGTG TCCCAGTGGG 
ATGACTTTGG ATGCCACAGG GAGGATTTGC CTAGATATCC GCCTGGAAAC CTGCTTCCTG 
AGGTACGAGG ACGAGGAGTG CACCCTGCCC GTGGCTGGGC GCCACCGCAT GGACGCCTGC 
TGCTGCTCAG TCGGGGCAGC CTGGGGCACC GAGGAGTGTG AGGAGTGTCC CGTGAGAAAC 
ACACCTGAGT ACGAGGAGCT CTGTCCCAGA GGACCCGGAT TCGCCACAAA AGAAATTACA 
AATGGCAAAC GTTTTTTCAA AGACATCAAC GAGTGTAAGA TGATTCCCAA CCTGTGCACC 
CACGGCAAGT GCAGGAACAC CATTGGCAGC TTCAAGTGCA GGTGTGACAG CGGCTTTGCT 
CTGGATTCTG AAGAAAGGAA CTGCACAGAC ATCGACGAAT GCCGCATATC CCCTGACCTC 
TGTGGCCGAG GCCAGTGTGT GAACACCCCC GGGGACTTTG AGTGCAAGTG CGATGAAGGC 
TATGAAAGCG GATTCATGAT GATGAAGAAC TGCATGGATA TCGATGAGTG TCAGAGAGAT 
CCGCTGTTGT GCCGAGGAGG CGTGTGCCTG AACACTGAGG GAAGTTACCG CTGTGAATGT 
CCCCCTGGCC ATCAGCTGGC CCCCAACATC TCGGCGTGTA TAGACATCAA CGAGTGTGAA 
CTAAGTGCAC ACCTCTGCCC CCATGGCCGC TGCGTGAACC TCATAGGAAA GTACCAGTGT 
GCCTGCAACC CTGGCTACCA CTCTACCCCT GACAGGCTGT TTTGTGTTGA CATTGATGAA 
TGCAGCATAA TGAACGGTGG CTGTGAGACC TTCTGCACAA ACTCTGAAGG TAGCTACGAA 
TGTAGCTGTC AGCCAGGATT TGCACTGATG CCTGACCAGA GGTCCTGCAC AGACATTGAT 
GAGTGTGAAG ATAACCCCAA TATCTGTGAT GGTGGTCAGT GTACAAACAT ACCTGGAGAG 
TACAGGTGCT TGTGTTATGA TGGATTCATG GCATCTGAAG ACATGAAGAC TTGCGTAGAT 
GTCAATGAAT GTGATCTGAA TCCAAATATC TGCCTAAGTG GAACCTGTGA AAACACCAAA 
GGCTCATTTA TCTGCCACTG TGATATGGGC TATTCAGGCA AAAAAGGAAA AACAGGCTGT 
ACAGACATCA ATGAATGTGA AATTGGAGCA CACAACTGCG ACAGACATGC TGTATGTACC 
AACACAGCAG GAAGCTTCAA ATGCAGCTGT AGCCCCGGGT GGATTGGAGA TGGCATTAAG 
TGCACTGACC TGGACGAATG CTCCAATGGA ACCCACATGT GCAGCCAACA TGCAGACTGC 
AAGAACACCA TGGGCTCCTA CCGCTGCCTC TGCAAAGAGG GGTACACAGG CGATGGCTTC 
ACTTGTACAG ATCTTGATGA GTGCTCTGAG AATCTAAACC TGTGTGGCAA CGGCCAGTGC 
CTTAACGCCC CAGGAGGATA CCGCTGTGAA TGTGACATGG GCTTTGTGCC CAGTGCTGAT 
GGGAAAGCCT GTGAAGATAT CGATGAGTGC TCTCTTCCGA ACATCTGTGT TTTTGGAACT 
TGCCACAACC TCCCTGGCCT CTTCCGCTGT GAATGTGAGA TCGGCTATGA ACTGGACAGA 
AGTGGTGGGA ACTGCACAGA CGTTAATGAA TGCCTGGATC CAACCACGTG CATCAGTGGG 
AACTGTGTCA ATACTCCAGG CAGCTATACC TGTGACTGTC CACCCGATTT TGAGCTGAAT 
CCAACTCGAG TTGGCTGTGT CGATACCCGC TCTGGAAACT GCTATTTGGA TATCCGTCCT 
CGGGGAGACA ATGGAGATAC AGCCTGCAGC AATGAAATTG GGGTTGGAGT GTCCAAGGCT 
TCCTGCTGCT GCTCTCTGGG TAAAGCCTGG GGCACTCCTT GTGAGCTGTG CCCGCCTGTG 
AACACATCTG AGTACAAAAT TCTTTGTCCT GGAGGAGAAG GTTTTCGACC CAACCCTATC 
ACCGTTATAT TGGAAGATAT TGATGAGTGC CAGGAGCTTC CAGGGTTGTG CCAAGGCGGG 
AAATGTATCA ATACCTTCGG CAGCTTCCAG TGCCGCTGTC CAACGGGCTA CTACCTGAAT 
GAAGATACCC GAGTGTGTGA TGATGTGAAT GAATGTGAAA CTCCCGGAAT CTGTGGTCCA 
GGGACATGTT ACAACACTGT TGGCAACTAC ACCTGTATCT GCCCTCCAGA CTACATGCAA 
GTGAATGGGG GGAATAATTG CATGGATATG AGGAGGAGTC TGTGCTACAG GAACTATTAT 
GCTGACAACC AGACCTGCGA TGGAGAACTG TTGTTCAACA TGACCAAGAA GATGTGCTGT 
TGTTCCTACA ACATTGGCCG GGCCTGGAAC AAGCCCTGTG AACAGTGCCC CATTCCAAGC 
ACAGATGAAT TTGCTACACT CTGTGGAAGT CAACGGCCAG GCTTTGTCAT CGACATCTAT 
ACCGGTTTAC CTGTCGATAT CGACGAGTGT CGGGAGATCC CAGGGGTCTG TGAAAACGGT 
GTGTGTATCA ACATGGTCGG CAGCTTCCGT TGTGAATGTC CAGTGGGCTT CTTCTATAAT 
GACAAGTTGC TGGTTTGTGA AGACATTGAC GAGTGTCAGA ACGGCCCAGT GTGCCAGCGA 
AACGCAGAGT GCATCAACAC CGCGGGCAGC TACCGCTGTG ATTGCAAACC CGGCTACCGA 
TTCACCTCCA CGGGGCAGTG CAATGATCGC AATGAATGTC AAGAAATCCC CAATATATGC 
AGCCATGGAC AGTGTATTGA CACAGTTGGA AGCTTTTATT GCCTTTGCCA CACTGGTTTT 
AAAACAAACG CTGATCAAAC CATGTGTTTG GACATAAATG AGTGTGAAAG AGATGCCTGT 
GGGAATGGCA CTTGCCGCAA CACGATTGGT TCCTTCAACT GCCGCTGCAA TCACGGTTTC 
ATCCTTTCCC ACAACAATGA CTGCATAGAT GTTGATGAAT GTGCAACTGG AAATGGGAAC 
CTTTGCAGAA ATGGCCAGTG TATCAATACA GTGGGGTCCT TCCAGTGTCA GTGCAATGAA 
GGCTATGAGG TGGCTCCAGA TGGGAGAACC TGTGTGGATA TCAATGAATG TCTCCTAGAT 
CCCAGAAAAT GTGCCCCAGG CACCTGTCAA AACTTGGATG GGTCCTACAG ATGTATCTGT 
CCACCTGGAT ACAGTCTCCA GAATGACAAG TGTGAAGATA TTGATGAATG TGTTGAAGAG 
CCAGAAATCT GTGCCTTGGG CACGTGCAGT AACACTGAAG GCAGCTTCAA ATGTCTGTGT 
CCAGACGGGT TCTCCTTGTC CTCCACTGGA AGAAGGTGCC AAGATTTACG AATGAGCTAC 
TGTTACGCGA AGTTTGAAGG AGGAAAGTGT TCATCACCCA AATCCAGAAA TCACTCCAAA 
CAGGAATGCT GCTGTGCCTT GAAGGGAGAA GGTTGGGGAG ATCCCTGCGA GCTGTGCCCC 
ACTGAACCCG ATGAGGCCTT CCGCCAGATC TGTCCCTATG GAAGTGGCAT CATCGTGGGA 
CCTGATGATT CAGCAGTTGA CATGGATGAG TGCAAAGAAC CCGATGTCTG TAAACACGGG 
CAGTGCATCA ACACCGATGG GTCCTATCGC TGCGAATGTC CCTTTGGCTA CATTCTGCAA 
GGGAATGAAT GTGTGGACAC TGATGAATGT TCGGTAGGCA ATCCTTGTGG AAATGGAACC 
TGCAAGAATG TGATTGGAGG TTTCGAATGC ACCTGTGAGG AGGGATTTGA GCCTGGGCCA 
ATGATGACTT GTGAAGACAT AAATGAGTGT GCCCAGAATC CTCTGCTCTG TGCCTTCCGC 
TGTGTAAATA CTTATGGGTC ATATGAATGC AAGTGTCCTG CTGGATATGT TCTGAGAGAA 
GACAGAAGGA TGTGCAAAGA TGAGGATGAG TGTGAAGAGG GAAAACACGA CTGTGCTGAA 
AAACAAATGG AGTGCAAGAA CCTCATTGGC ACATACCTCT GCATCTGTGG ACCTGGGTAT 
CAGCGAAGAC CTGACGGAGA AGGCTGCGTA GATGAAAATG AATGTCAGAC CAAGCCTGGG 
ATCTGTGAGA ACGGGCGCTG CCTCAACACC AGGGGGAGCT ATACCTGCGA GTGCAATGAC 
GGCTTTACAG CCAGCCCCAA CCAGGATGAG TGCCTCGACA ACCGAGAAGG GTACTGCTTC 
ACGGAGGTGC TCCAGAACAT GTGTCAGATC GGCTCAAGCA ACAGGAACCC CGTCACTAAG 
TCTGAATGCT GCTGCGATGG AGGGAGAGGC TGGGGGCCCC ACTGTGAGAT CTGCCCTTTC 
CAGGGCACTG TGGCTTTCAA AAAACTCTGC CCCCATGGCC GAGGGTTTAT GACCAATGGA 
GCAGATATTG ATGAGTGCAA GGTTATTCAT GATGTTTGCC GAAATGGGGA ATGTGTCAAT 
GACAGAGGAT CATATCATTG CATTTGTAAA ACTGGCTACA CTCCAGATAT AACTGGAACT 
GCCTGTGTAG ATCTAAATGA GTGCAACCAG GCTCCCAAAC CCTGCAATTT CATCTGTAAG 
AACACAGAAG GGAGTTACCA GTGTTCATGC CCGAAAGGCT ACATTCTGCA GGAGGACGGA 
AGGAGCTGCA AAGATCTTGA TGAGTGTGCA ACTAAGCAGC ACAACTGCCA GTTCCTGTGT 
GTTAACACCA TTGGCAGCTT CACATGTAAA TGTCCTCCTG GATTTACCCA ACACCATACT 
GCCTGCATTG ATAACAATGA ATGCACCTCT GACATCAACC TGTGTGGGTC AAAGGGCATT 
TGCCAGAACA CTCCTGGAAG CTTCACTTGT GAATGCCAGC GGGGATTCTC ACTTGATCCG 
AGCGGCGCAA GCTGTGAAGA TGTGGACGAG TGTGAGGGAA ACCATCGCTG CCAGCACGGC 
TGCCAGAACA TCATCGGCGG CTACAGGTGC AGCTGCCCCC AGGGCTATCT CCAGCACTAC 
CAGTGGAACC AGTGTGTTGA TGAAAATGAA TGCCTCAGTG CTCACATCTG TGGAGGAGCC 
TCCTGTCACA ACACCCTGGG GAGCTACAAG TGCATGTGCC CTGCCGGCTT CCAATACGAA 
CAGTTCAGTG GAGGTTGCCA AGACATCAAC GAATGTGGCT CTGCTCAGGC CCCCTGCAGT 
TATGGCTGTT CCAATACTGA GGGCGGCTAC CTGTGTGCCT GTCCACCTGG CTACTTCCGA 
ATAGGCCAAG GGCACTGTGT TTCTGGAATG GGCATGGGCC GGGGAAACCC AGAGCCACCT 
GCCAGCGGTG AAATGGATGA CAACTCACTC TCCCCAGAGG CTTGTTATGA GTGTAAGATC 
AACGGCTACC CCAAACGCGG CAGGAAGCGG AGAAGCGCAA ATGAAACAGA TGCCTCCAAC 
ATTGAGGATC AACCTGAGAT AGAAGCCAAT GTGAGTCTTG CAAGTTGGGA TGTTGAGAAG 
ACAGCCGTGT TCGCTTTCAA TATTTCCCAC ATCAGTAACA AGGTCCGAAT CCTAGAACTC 
CTTCCAGCCC TCACGACTCT GACGAATCAC AACAGATACT TGATTGAATC TGGAAATGAA 
AACGGCTTCT TTAAAATCAA CCAAAAGGAA GGGATCAGCT ACCTCCACTT CACAAAGAAG 
AAGCCAGTGG CTGGAACCTA TTCATTACAA ATCAGTAGTA CTCCACTTTA TAAAAAGAAA 
GAACTTAATC AGCTAGAAGA TAAATATGAC AAAGACTACC TCAGTGGTGA ACTGGGTGAT 
AATCTGAAGA TGAAAATTCA GATTTTGCTT CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of an FBN1 mutation in bovine Marfan syndrome-like disease.
Animal genetics43(1)11-7(2012 Feb)
Hirano T,Matsuhashi T,Kobayashi N,Watanabe T,Sugimoto Y

Microfibrils and fibrillin-1 induce integrin-mediated signaling, proliferation and migration in human endothelial cells.
American journal of physiology. Cell physiology299(5)C977-87(2010 Nov)
Mariko B,Ghandour Z,Raveaud S,Quentin M,Usson Y,Verdetti J,Huber P,Kielty C,Faury G

Fibrillins and latent TGFbeta binding proteins in bovine ovaries of offspring following high or low protein diets during pregnancy of dams.
Molecular and cellular endocrinology307(1-2)133-41(2009 Aug)
Prodoehl MJ,Irving-Rodgers HF,Bonner WM,Sullivan TM,Micke GC,Gibson MA,Perry VE,Rodgers RJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Bovine model of Marfan syndrome results from an amino acid change (c.3598G > A, p.E1200K) in a calcium-binding epidermal growth factor-like domain of fibrillin-1.
Human mutation25(4)348-52(2005 Apr)
Singleton AC,Mitchell AL,Byers PH,Potter KA,Pace JM

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FBN1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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