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PLCZ1 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol PLCZ1
Entrez Gene ID 497026
Full Name phospholipase C zeta 1
Synonyms PLCZ
General protein information
Preferred Names

phospholipase C zeta 1

Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
PLC-zeta-1
phosphoinositide phospholipase C-zeta-1
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001011680.3 NP_001011680.2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
XM_024991429.1 XP_024847197.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1
XM_024991430.1 XP_024847198.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) PLCZ1 XP_005637152.1
Homo sapiens (human) PLCZ1 NP_149114.2
Mus musculus (house mouse) Plcz1 NP_473407.2
Rattus norvegicus (Norway rat) Plcz1 NP_001012234.1
Bos taurus (cattle) PLCZ1 NP_001011680.2
Gallus gallus (chicken) PLCZ1 NP_001034362.1
Pan troglodytes (chimpanzee) PLCZ1 XP_001160159.1
PLCZ1 XP_001095846.2

Gene Ontology


Bibliography

Related articles in PubMed

During capacitation in bull spermatozoa, actin and PLC-? undergo dynamic interactions.
Mej?a-Flores I, Chiquete-F?lix N, Palma-Lara I, Uribe-Carvajal S, de Lourdes Ju?rez-Mosqueda M
Zygote (Cambridge, England)25(5)558-566(2017 Oct)

Identification and characterization of a novel splice variant of the PLC?1 gene in bull testis tissues.
Ju ZH, Pan Q, Zhang Y, Huang JM, Qi C, Wang XG, Li QL, Zhong JF, Liu M, Wang CF
Genetics and molecular research : GMR13(4)9899-909(2014 Nov)

Species-specific differences in the activity and nuclear localization of murine and bovine phospholipase C zeta 1.
Cooney MA, Malcuit C, Cheon B, Holland MK, Fissore RA, D'Cruz NT
Biology of reproduction83(1)92-101(2010 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel splice variant of Phospholipase C zeta 1 in the testis tissues of adult and fetal Chinese Holstein bulls was characterized.
Title: Identification and characterization of a novel splice variant of the PLC?1 gene in bull testis tissues.

Murine PLCZ1 and bovine PLCZ1 possess species-specific differences in activity and suggest potential differences in the mode of action of the protein between the two species
Title: Species-specific differences in the activity and nuclear localization of murine and bovine phospholipase C zeta 1.

The following PLCZ1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCZ1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa19677 NM_001011680.3
Latest version!		 Bos taurus phospholipase C zeta 1 (PLCZ1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa19677 XM_024991429.1
Latest version!		 Bos taurus phospholipase C zeta 1 (PLCZ1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa19678 XM_024991430.1
Latest version!		 Bos taurus phospholipase C zeta 1 (PLCZ1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa19677
Clone ID Related Accession (Same CDS sequence) NM_001011680.3 , XM_024991429.1
Accession Version NM_001011680.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-19
Organism Bos taurus(cattle)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114836.1. On Aug 30, 2012 this sequence version replaced NM_001011680.2. ##Evidence-Data-START## Transcript exon combination :: BC114836.1, AY646356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAGAACA AATGGTTTTT GTTGATGGTT CGAGATGACT TCAAAGGTGG AAAAATTACT 
CTAGAAAAAG CTCTGAAACT ACTTGAAAAA TTAGATATTC AGTGCAATAC TATTCATGTG 
AAATATATTT TTAAGGATAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT 
AGAACAATTT ATCGAATTAT TACATACAGA GAAGAAATTA TTGAGATTTT CAACACGTAT 
TCTGAAAACC GGAAAATTCT TTTAGAAAAG AACCTGGTCG AATTTTTGAT GCGAGAACAG 
TATACACTTG ACTTCAATAA AAGTATTGCT TCTGAGATCA TTCAGAAATA TGAGCCTATT 
GAAGAAGTTA AACAAGCACA CCAGATGTCA TTTGAAGGTT TTAGAAGATA CATGGATTCA 
TCTGAATGTC TGCTATTTGA TAATAAGTGT GATCATGTTT ACCAAGATAT GACTCATCCA 
TTGACTGATT ATTTTATTTC AAGTTCACAT AATACATACT TGATATCTGA CCAATTATGG 
GGACCAAGTG ACCTTTGGGG ATATATAAGT GCCCTTGTGA AAGGATGCCG CTGTCTGGAA 
ATTGACTGCT GGGATGGATC ACAAAATGAA CCTGTTGTAT ACCATGGCTA TACATTCACC 
AGCAAGCTTC TGTTTAAAAC TGTTATCCAA GCTATAAACA AATATGCATT CCTAGCATCT 
GAGTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCC CTTCCCAACA AGAAGTGATG 
GCCGACAGTT TGCTCGCTAC ATTTGGGGAT GCCTTGCTGT CATATACGCT TGATAATTTT 
TCAGACAGAC TACCTTCACC AGAGGCATTA AAATTCAAAA TATTAGTTAG AAACAAGAAA 
ATAGGAACCT TACATGAAAC CCTTGAAAGG AAAGGTTCTG ATATGCATGG GAAGGTAGAG 
GAATTTGAAG AAGAGGAGGA GATAGAACAA GAGGAGGATG GAAGTGGTGC CAAAGAACCA 
GAACCAGTGG GTGATTTTCA AGATGATTTG GCAAAGGAAG AACAGTTGAA ACGGGTGGTC 
GGAATCCCAC TCTTCAGGAA AAAGAAGATA AAAATATCTA TGGCCCTATC TGATCTTGTT 
ATTTATACTA AAGTTGAGAA GTTCAAAAGT TTTCATCATT CACATCTATA TCAACAATTT 
AATGAAAGCA ACTCTATTGG GGAGTCCCAA GCCCGAAAAC TTACAAAGTT GGCAGCCAGG 
GAATTTATTC TTCACACCAG GAGGTTCATT ACCAGAGTAT ATCCCAAAGC ATTAAGAGCT 
GACTCTTCTA ATTTTAATCC TCAAGAATTT TGGAATGTAG GTTGTCAAAT GGTGGCCTTA 
AATTTCCAGA CCCCTGGTGT CCCTATGGAT CTTCAAAATG GGAAATTTTT GGATAATGGT 
TGTTCTGGAT ATGTTTTAAA ACCACGCTTC CTAAGAGATA AAAAAACAAA GTTTAATCCA 
CATAAAGTAC AAATAGACAG TAATCCACTC ACACTTACAA TAAGGCTCAT CAGTGGTATC 
CAGTTGCCTC CCAGTTATCA AAACAAAGCT GATACCTTAG TGATTGTAGA AATTTTTGGG 
GTTCCAAATG ATCAAATGAA GCAGCAGAGT CGTGTAATTA AAAAAAATGC TTTTAATCCA 
AGATGGAATG AAACATTCAC ATTTGTTATT CAGGTGCCAG AATTAGCATT GATTCGTTTT 
GTTGCTGAAA ATCAAGGTTT AATAGCTGGA AATGAATTTC TTGGACAATA TACTTTACCA 
GTTCTATGCA TGAACAGAGG TTACCGTCGT GTTCCTCTGT TTTCCAAAAT GGGTGAGAGT 
CTTGAGCCCG CTTCCCTTTT TATTTATGTT TGGTACATCA GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001011680.2
CDS271..2175
Translation

Target ORF information:

RefSeq Version NM_001011680.3
Organism Bos taurus(cattle)
Definition Bos taurus phospholipase C zeta 1 (PLCZ1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001011680.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAGAACA AATGGTTTTT GTTGATGGTT CGAGATGACT TCAAAGGTGG AAAAATTACT 
CTAGAAAAAG CTCTGAAACT ACTTGAAAAA TTAGATATTC AGTGCAATAC TATTCATGTG 
AAATATATTT TTAAGGATAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT 
AGAACAATTT ATCGAATTAT TACATACAGA GAAGAAATTA TTGAGATTTT CAACACGTAT 
TCTGAAAACC GGAAAATTCT TTTAGAAAAG AACCTGGTCG AATTTTTGAT GCGAGAACAG 
TATACACTTG ACTTCAATAA AAGTATTGCT TCTGAGATCA TTCAGAAATA TGAGCCTATT 
GAAGAAGTTA AACAAGCACA CCAGATGTCA TTTGAAGGTT TTAGAAGATA CATGGATTCA 
TCTGAATGTC TGCTATTTGA TAATAAGTGT GATCATGTTT ACCAAGATAT GACTCATCCA 
TTGACTGATT ATTTTATTTC AAGTTCACAT AATACATACT TGATATCTGA CCAATTATGG 
GGACCAAGTG ACCTTTGGGG ATATATAAGT GCCCTTGTGA AAGGATGCCG CTGTCTGGAA 
ATTGACTGCT GGGATGGATC ACAAAATGAA CCTGTTGTAT ACCATGGCTA TACATTCACC 
AGCAAGCTTC TGTTTAAAAC TGTTATCCAA GCTATAAACA AATATGCATT CCTAGCATCT 
GAGTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCC CTTCCCAACA AGAAGTGATG 
GCCGACAGTT TGCTCGCTAC ATTTGGGGAT GCCTTGCTGT CATATACGCT TGATAATTTT 
TCAGACAGAC TACCTTCACC AGAGGCATTA AAATTCAAAA TATTAGTTAG AAACAAGAAA 
ATAGGAACCT TACATGAAAC CCTTGAAAGG AAAGGTTCTG ATATGCATGG GAAGGTAGAG 
GAATTTGAAG AAGAGGAGGA GATAGAACAA GAGGAGGATG GAAGTGGTGC CAAAGAACCA 
GAACCAGTGG GTGATTTTCA AGATGATTTG GCAAAGGAAG AACAGTTGAA ACGGGTGGTC 
GGAATCCCAC TCTTCAGGAA AAAGAAGATA AAAATATCTA TGGCCCTATC TGATCTTGTT 
ATTTATACTA AAGTTGAGAA GTTCAAAAGT TTTCATCATT CACATCTATA TCAACAATTT 
AATGAAAGCA ACTCTATTGG GGAGTCCCAA GCCCGAAAAC TTACAAAGTT GGCAGCCAGG 
GAATTTATTC TTCACACCAG GAGGTTCATT ACCAGAGTAT ATCCCAAAGC ATTAAGAGCT 
GACTCTTCTA ATTTTAATCC TCAAGAATTT TGGAATGTAG GTTGTCAAAT GGTGGCCTTA 
AATTTCCAGA CCCCTGGTGT CCCTATGGAT CTTCAAAATG GGAAATTTTT GGATAATGGT 
TGTTCTGGAT ATGTTTTAAA ACCACGCTTC CTAAGAGATA AAAAAACAAA GTTTAATCCA 
CATAAAGTAC AAATAGACAG TAATCCACTC ACACTTACAA TAAGGCTCAT CAGTGGTATC 
CAGTTGCCTC CCAGTTATCA AAACAAAGCT GATACCTTAG TGATTGTAGA AATTTTTGGG 
GTTCCAAATG ATCAAATGAA GCAGCAGAGT CGTGTAATTA AAAAAAATGC TTTTAATCCA 
AGATGGAATG AAACATTCAC ATTTGTTATT CAGGTGCCAG AATTAGCATT GATTCGTTTT 
GTTGCTGAAA ATCAAGGTTT AATAGCTGGA AATGAATTTC TTGGACAATA TACTTTACCA 
GTTCTATGCA TGAACAGAGG TTACCGTCGT GTTCCTCTGT TTTCCAAAAT GGGTGAGAGT 
CTTGAGCCCG CTTCCCTTTT TATTTATGTT TGGTACATCA GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa19677
Clone ID Related Accession (Same CDS sequence) NM_001011680.3 , XM_024991429.1
Accession Version XM_024991429.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAGAACA AATGGTTTTT GTTGATGGTT CGAGATGACT TCAAAGGTGG AAAAATTACT 
CTAGAAAAAG CTCTGAAACT ACTTGAAAAA TTAGATATTC AGTGCAATAC TATTCATGTG 
AAATATATTT TTAAGGATAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT 
AGAACAATTT ATCGAATTAT TACATACAGA GAAGAAATTA TTGAGATTTT CAACACGTAT 
TCTGAAAACC GGAAAATTCT TTTAGAAAAG AACCTGGTCG AATTTTTGAT GCGAGAACAG 
TATACACTTG ACTTCAATAA AAGTATTGCT TCTGAGATCA TTCAGAAATA TGAGCCTATT 
GAAGAAGTTA AACAAGCACA CCAGATGTCA TTTGAAGGTT TTAGAAGATA CATGGATTCA 
TCTGAATGTC TGCTATTTGA TAATAAGTGT GATCATGTTT ACCAAGATAT GACTCATCCA 
TTGACTGATT ATTTTATTTC AAGTTCACAT AATACATACT TGATATCTGA CCAATTATGG 
GGACCAAGTG ACCTTTGGGG ATATATAAGT GCCCTTGTGA AAGGATGCCG CTGTCTGGAA 
ATTGACTGCT GGGATGGATC ACAAAATGAA CCTGTTGTAT ACCATGGCTA TACATTCACC 
AGCAAGCTTC TGTTTAAAAC TGTTATCCAA GCTATAAACA AATATGCATT CCTAGCATCT 
GAGTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCC CTTCCCAACA AGAAGTGATG 
GCCGACAGTT TGCTCGCTAC ATTTGGGGAT GCCTTGCTGT CATATACGCT TGATAATTTT 
TCAGACAGAC TACCTTCACC AGAGGCATTA AAATTCAAAA TATTAGTTAG AAACAAGAAA 
ATAGGAACCT TACATGAAAC CCTTGAAAGG AAAGGTTCTG ATATGCATGG GAAGGTAGAG 
GAATTTGAAG AAGAGGAGGA GATAGAACAA GAGGAGGATG GAAGTGGTGC CAAAGAACCA 
GAACCAGTGG GTGATTTTCA AGATGATTTG GCAAAGGAAG AACAGTTGAA ACGGGTGGTC 
GGAATCCCAC TCTTCAGGAA AAAGAAGATA AAAATATCTA TGGCCCTATC TGATCTTGTT 
ATTTATACTA AAGTTGAGAA GTTCAAAAGT TTTCATCATT CACATCTATA TCAACAATTT 
AATGAAAGCA ACTCTATTGG GGAGTCCCAA GCCCGAAAAC TTACAAAGTT GGCAGCCAGG 
GAATTTATTC TTCACACCAG GAGGTTCATT ACCAGAGTAT ATCCCAAAGC ATTAAGAGCT 
GACTCTTCTA ATTTTAATCC TCAAGAATTT TGGAATGTAG GTTGTCAAAT GGTGGCCTTA 
AATTTCCAGA CCCCTGGTGT CCCTATGGAT CTTCAAAATG GGAAATTTTT GGATAATGGT 
TGTTCTGGAT ATGTTTTAAA ACCACGCTTC CTAAGAGATA AAAAAACAAA GTTTAATCCA 
CATAAAGTAC AAATAGACAG TAATCCACTC ACACTTACAA TAAGGCTCAT CAGTGGTATC 
CAGTTGCCTC CCAGTTATCA AAACAAAGCT GATACCTTAG TGATTGTAGA AATTTTTGGG 
GTTCCAAATG ATCAAATGAA GCAGCAGAGT CGTGTAATTA AAAAAAATGC TTTTAATCCA 
AGATGGAATG AAACATTCAC ATTTGTTATT CAGGTGCCAG AATTAGCATT GATTCGTTTT 
GTTGCTGAAA ATCAAGGTTT AATAGCTGGA AATGAATTTC TTGGACAATA TACTTTACCA 
GTTCTATGCA TGAACAGAGG TTACCGTCGT GTTCCTCTGT TTTCCAAAAT GGGTGAGAGT 
CTTGAGCCCG CTTCCCTTTT TATTTATGTT TGGTACATCA GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024847197.1
CDS279..2183
Translation

Target ORF information:

RefSeq Version XM_024991429.1
Organism Bos taurus(cattle)
Definition Bos taurus phospholipase C zeta 1 (PLCZ1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024991429.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAGAACA AATGGTTTTT GTTGATGGTT CGAGATGACT TCAAAGGTGG AAAAATTACT 
CTAGAAAAAG CTCTGAAACT ACTTGAAAAA TTAGATATTC AGTGCAATAC TATTCATGTG 
AAATATATTT TTAAGGATAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT 
AGAACAATTT ATCGAATTAT TACATACAGA GAAGAAATTA TTGAGATTTT CAACACGTAT 
TCTGAAAACC GGAAAATTCT TTTAGAAAAG AACCTGGTCG AATTTTTGAT GCGAGAACAG 
TATACACTTG ACTTCAATAA AAGTATTGCT TCTGAGATCA TTCAGAAATA TGAGCCTATT 
GAAGAAGTTA AACAAGCACA CCAGATGTCA TTTGAAGGTT TTAGAAGATA CATGGATTCA 
TCTGAATGTC TGCTATTTGA TAATAAGTGT GATCATGTTT ACCAAGATAT GACTCATCCA 
TTGACTGATT ATTTTATTTC AAGTTCACAT AATACATACT TGATATCTGA CCAATTATGG 
GGACCAAGTG ACCTTTGGGG ATATATAAGT GCCCTTGTGA AAGGATGCCG CTGTCTGGAA 
ATTGACTGCT GGGATGGATC ACAAAATGAA CCTGTTGTAT ACCATGGCTA TACATTCACC 
AGCAAGCTTC TGTTTAAAAC TGTTATCCAA GCTATAAACA AATATGCATT CCTAGCATCT 
GAGTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCC CTTCCCAACA AGAAGTGATG 
GCCGACAGTT TGCTCGCTAC ATTTGGGGAT GCCTTGCTGT CATATACGCT TGATAATTTT 
TCAGACAGAC TACCTTCACC AGAGGCATTA AAATTCAAAA TATTAGTTAG AAACAAGAAA 
ATAGGAACCT TACATGAAAC CCTTGAAAGG AAAGGTTCTG ATATGCATGG GAAGGTAGAG 
GAATTTGAAG AAGAGGAGGA GATAGAACAA GAGGAGGATG GAAGTGGTGC CAAAGAACCA 
GAACCAGTGG GTGATTTTCA AGATGATTTG GCAAAGGAAG AACAGTTGAA ACGGGTGGTC 
GGAATCCCAC TCTTCAGGAA AAAGAAGATA AAAATATCTA TGGCCCTATC TGATCTTGTT 
ATTTATACTA AAGTTGAGAA GTTCAAAAGT TTTCATCATT CACATCTATA TCAACAATTT 
AATGAAAGCA ACTCTATTGG GGAGTCCCAA GCCCGAAAAC TTACAAAGTT GGCAGCCAGG 
GAATTTATTC TTCACACCAG GAGGTTCATT ACCAGAGTAT ATCCCAAAGC ATTAAGAGCT 
GACTCTTCTA ATTTTAATCC TCAAGAATTT TGGAATGTAG GTTGTCAAAT GGTGGCCTTA 
AATTTCCAGA CCCCTGGTGT CCCTATGGAT CTTCAAAATG GGAAATTTTT GGATAATGGT 
TGTTCTGGAT ATGTTTTAAA ACCACGCTTC CTAAGAGATA AAAAAACAAA GTTTAATCCA 
CATAAAGTAC AAATAGACAG TAATCCACTC ACACTTACAA TAAGGCTCAT CAGTGGTATC 
CAGTTGCCTC CCAGTTATCA AAACAAAGCT GATACCTTAG TGATTGTAGA AATTTTTGGG 
GTTCCAAATG ATCAAATGAA GCAGCAGAGT CGTGTAATTA AAAAAAATGC TTTTAATCCA 
AGATGGAATG AAACATTCAC ATTTGTTATT CAGGTGCCAG AATTAGCATT GATTCGTTTT 
GTTGCTGAAA ATCAAGGTTT AATAGCTGGA AATGAATTTC TTGGACAATA TACTTTACCA 
GTTCTATGCA TGAACAGAGG TTACCGTCGT GTTCCTCTGT TTTCCAAAAT GGGTGAGAGT 
CTTGAGCCCG CTTCCCTTTT TATTTATGTT TGGTACATCA GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa19678
Clone ID Related Accession (Same CDS sequence) XM_024991430.1
Accession Version XM_024991430.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1617bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCGAGAAC AGTATACACT TGACTTCAAT AAAAGTATTG CTTCTGAGAT CATTCAGAAA 
TATGAGCCTA TTGAAGAAGT TAAACAAGCA CACCAGATGT CATTTGAAGG TTTTAGAAGA 
TACATGGATT CATCTGAATG TCTGCTATTT GATAATAAGT GTGATCATGT TTACCAAGAT 
ATGACTCATC CATTGACTGA TTATTTTATT TCAAGTTCAC ATAATACATA CTTGATATCT 
GACCAATTAT GGGGACCAAG TGACCTTTGG GGATATATAA GTGCCCTTGT GAAAGGATGC 
CGCTGTCTGG AAATTGACTG CTGGGATGGA TCACAAAATG AACCTGTTGT ATACCATGGC 
TATACATTCA CCAGCAAGCT TCTGTTTAAA ACTGTTATCC AAGCTATAAA CAAATATGCA 
TTCCTAGCAT CTGAGTACCC AGTGGTGCTC TCTTTAGAAA ATCACTGCTC CCCTTCCCAA 
CAAGAAGTGA TGGCCGACAG TTTGCTCGCT ACATTTGGGG ATGCCTTGCT GTCATATACG 
CTTGATAATT TTTCAGACAG ACTACCTTCA CCAGAGGCAT TAAAATTCAA AATATTAGTT 
AGAAACAAGA AAATAGGAAC CTTACATGAA ACCCTTGAAA GGAAAGGTTC TGATATGCAT 
GGGAAGGTAG AGGAATTTGA AGAAGAGGAG GAGATAGAAC AAGAGGAGGA TGGAAGTGGT 
GCCAAAGAAC CAGAACCAGT GGGTGATTTT CAAGATGATT TGGCAAAGGA AGAACAGTTG 
AAACGGGTGG TCGGAATCCC ACTCTTCAGG AAAAAGAAGA TAAAAATATC TATGGCCCTA 
TCTGATCTTG TTATTTATAC TAAAGTTGAG AAGTTCAAAA GTTTTCATCA TTCACATCTA 
TATCAACAAT TTAATGAAAG CAACTCTATT GGGGAGTCCC AAGCCCGAAA ACTTACAAAG 
TTGGCAGCCA GGGAATTTAT TCTTCACACC AGGAGGTTCA TTACCAGAGT ATATCCCAAA 
GCATTAAGAG CTGACTCTTC TAATTTTAAT CCTCAAGAAT TTTGGAATGT AGGTTGTCAA 
ATGGTGGCCT TAAATTTCCA GACCCCTGGT GTCCCTATGG ATCTTCAAAA TGGGAAATTT 
TTGGATAATG GTTGTTCTGG ATATGTTTTA AAACCACGCT TCCTAAGAGA TAAAAAAACA 
AAGTTTAATC CACATAAAGT ACAAATAGAC AGTAATCCAC TCACACTTAC AATAAGGCTC 
ATCAGTGGTA TCCAGTTGCC TCCCAGTTAT CAAAACAAAG CTGATACCTT AGTGATTGTA 
GAAATTTTTG GGGTTCCAAA TGATCAAATG AAGCAGCAGA GTCGTGTAAT TAAAAAAAAT 
GCTTTTAATC CAAGATGGAA TGAAACATTC ACATTTGTTA TTCAGGTGCC AGAATTAGCA 
TTGATTCGTT TTGTTGCTGA AAATCAAGGT TTAATAGCTG GAAATGAATT TCTTGGACAA 
TATACTTTAC CAGTTCTATG CATGAACAGA GGTTACCGTC GTGTTCCTCT GTTTTCCAAA 
ATGGGTGAGA GTCTTGAGCC CGCTTCCCTT TTTATTTATG TTTGGTACAT CAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024847198.1
CDS112..1728
Translation

Target ORF information:

RefSeq Version XM_024991430.1
Organism Bos taurus(cattle)
Definition Bos taurus phospholipase C zeta 1 (PLCZ1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024991430.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCGAGAAC AGTATACACT TGACTTCAAT AAAAGTATTG CTTCTGAGAT CATTCAGAAA 
TATGAGCCTA TTGAAGAAGT TAAACAAGCA CACCAGATGT CATTTGAAGG TTTTAGAAGA 
TACATGGATT CATCTGAATG TCTGCTATTT GATAATAAGT GTGATCATGT TTACCAAGAT 
ATGACTCATC CATTGACTGA TTATTTTATT TCAAGTTCAC ATAATACATA CTTGATATCT 
GACCAATTAT GGGGACCAAG TGACCTTTGG GGATATATAA GTGCCCTTGT GAAAGGATGC 
CGCTGTCTGG AAATTGACTG CTGGGATGGA TCACAAAATG AACCTGTTGT ATACCATGGC 
TATACATTCA CCAGCAAGCT TCTGTTTAAA ACTGTTATCC AAGCTATAAA CAAATATGCA 
TTCCTAGCAT CTGAGTACCC AGTGGTGCTC TCTTTAGAAA ATCACTGCTC CCCTTCCCAA 
CAAGAAGTGA TGGCCGACAG TTTGCTCGCT ACATTTGGGG ATGCCTTGCT GTCATATACG 
CTTGATAATT TTTCAGACAG ACTACCTTCA CCAGAGGCAT TAAAATTCAA AATATTAGTT 
AGAAACAAGA AAATAGGAAC CTTACATGAA ACCCTTGAAA GGAAAGGTTC TGATATGCAT 
GGGAAGGTAG AGGAATTTGA AGAAGAGGAG GAGATAGAAC AAGAGGAGGA TGGAAGTGGT 
GCCAAAGAAC CAGAACCAGT GGGTGATTTT CAAGATGATT TGGCAAAGGA AGAACAGTTG 
AAACGGGTGG TCGGAATCCC ACTCTTCAGG AAAAAGAAGA TAAAAATATC TATGGCCCTA 
TCTGATCTTG TTATTTATAC TAAAGTTGAG AAGTTCAAAA GTTTTCATCA TTCACATCTA 
TATCAACAAT TTAATGAAAG CAACTCTATT GGGGAGTCCC AAGCCCGAAA ACTTACAAAG 
TTGGCAGCCA GGGAATTTAT TCTTCACACC AGGAGGTTCA TTACCAGAGT ATATCCCAAA 
GCATTAAGAG CTGACTCTTC TAATTTTAAT CCTCAAGAAT TTTGGAATGT AGGTTGTCAA 
ATGGTGGCCT TAAATTTCCA GACCCCTGGT GTCCCTATGG ATCTTCAAAA TGGGAAATTT 
TTGGATAATG GTTGTTCTGG ATATGTTTTA AAACCACGCT TCCTAAGAGA TAAAAAAACA 
AAGTTTAATC CACATAAAGT ACAAATAGAC AGTAATCCAC TCACACTTAC AATAAGGCTC 
ATCAGTGGTA TCCAGTTGCC TCCCAGTTAT CAAAACAAAG CTGATACCTT AGTGATTGTA 
GAAATTTTTG GGGTTCCAAA TGATCAAATG AAGCAGCAGA GTCGTGTAAT TAAAAAAAAT 
GCTTTTAATC CAAGATGGAA TGAAACATTC ACATTTGTTA TTCAGGTGCC AGAATTAGCA 
TTGATTCGTT TTGTTGCTGA AAATCAAGGT TTAATAGCTG GAAATGAATT TCTTGGACAA 
TATACTTTAC CAGTTCTATG CATGAACAGA GGTTACCGTC GTGTTCCTCT GTTTTCCAAA 
ATGGGTGAGA GTCTTGAGCC CGCTTCCCTT TTTATTTATG TTTGGTACAT CAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

During capacitation in bull spermatozoa, actin and PLC-? undergo dynamic interactions.
Zygote (Cambridge, England)25(5)558-566(2017 Oct)
Mej?a-Flores I,Chiquete-F?lix N,Palma-Lara I,Uribe-Carvajal S,de Lourdes Ju?rez-Mosqueda M


book

Identification and characterization of a novel splice variant of the PLC?1 gene in bull testis tissues.
Genetics and molecular research : GMR13(4)9899-909(2014 Nov)
Ju ZH,Pan Q,Zhang Y,Huang JM,Qi C,Wang XG,Li QL,Zhong JF,Liu M,Wang CF


book

Species-specific differences in the activity and nuclear localization of murine and bovine phospholipase C zeta 1.
Biology of reproduction83(1)92-101(2010 Jul)
Cooney MA,Malcuit C,Cheon B,Holland MK,Fissore RA,D'Cruz NT


book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL