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PLCZ1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PLCZ1
Entrez Gene ID 89869
Full Name phospholipase C zeta 1
Synonyms NYD-SP27,PLC-zeta-1,PLCzeta,SPGF17
General protein information
Preferred Names

phospholipase C zeta 1

Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
PI-phospholipase C zeta 1
phosphoinositide phospholipase C-zeta-1
testicular tissue protein Li 145
testis-development related NYD-SP27

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

12

12p12.3

Summary The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012].
Disorder MIM:

608075


mRNA and Protein(s)

mRNA Protein Name
NM_001330774.1 NP_001317703.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform 2
XM_017020180.1 XP_016875669.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X5
NM_001330769.1 NP_001317698.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform 3
XM_017020176.1 XP_016875665.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1
XM_017020181.1 XP_016875670.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X5
XM_017020182.1 XP_016875671.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X6
XM_017020183.1 XP_016875672.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X7
XM_017020177.1 XP_016875666.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X3
XM_017020185.1 XP_016875674.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X10
XM_005253519.2 XP_005253576.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2
XM_017020178.1 XP_016875667.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X4
XM_017020184.1 XP_016875673.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X8
XM_017020186.1 XP_016875675.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X11
NM_033123.3 NP_149114.2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform 1
XM_017020179.1 XP_016875668.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X5



Canis lupus familiaris (dog) PLCZ1 XP_005637152.1
Homo sapiens (human) PLCZ1 NP_149114.2
Mus musculus (house mouse) Plcz1 NP_473407.2
Rattus norvegicus (Norway rat) Plcz1 NP_001012234.1
Bos taurus (cattle) PLCZ1 NP_001011680.2
Gallus gallus (chicken) PLCZ1 NP_001034362.1
Pan troglodytes (chimpanzee) PLCZ1 XP_001160159.1
Macaca mulatta (rhesus monkey) PLCZ1 XP_001095846.2


Related articles in PubMed

An association between sperm PLCζ levels and varicocele?
Janghorban-Laricheh E, Ghazavi-Khorasgani N, Tavalaee M, Zohrabi D, Abbasi H, Nasr-Esfahani MH
Journal of assisted reproduction and genetics33(12)1649-1655(2016 Dec)

Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.
Escoffier J, Lee HC, Yassine S, Zouari R, Martinez G, Karaouzène T, Coutton C, Kherraf ZE, Halouani L, Triki C, Nef S, Thierry-Mieg N, Savinov SN, Fissore R, Ray PF, Arnoult C
Human molecular genetics25(5)878-91(2016 Mar)

Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report.
Chithiwala ZH, Lee HC, Hill DL, Jellerette-Nolan T, Fissore R, Grow D, Dumesic DA
Journal of assisted reproduction and genetics32(9)1415-9(2015 Sep)

Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.
Nomikos M, Sanders JR, Kashir J, Sanusi R, Buntwal L, Love D, Ashley P, Sanders D, Knaggs P, Bunkheila A, Swann K, Lai FA
Molecular human reproduction21(9)702-10(2015 Sep)

NYD-SP27, a novel intrinsic decapacitation factor in sperm.
Bi Y, Xu WM, Wong HY, Zhu H, Zhou ZM, Chan HC, Sha JH
Asian journal of andrology11(2)229-39(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings highlight the importance of PLCzeta at fertilization and the vital role of the C2 domain in PLCzeta function, possibly due to its novel binding characteristics.
Title: Male infertility-linked point mutation reveals a vital binding role for the C2 domain of sperm PLCζ.

The findings of the present study illustrate that one of the etiologies of reduced fertility associated with varicocele is the low expression of PLCzeta
Title: An association between sperm PLCζ levels and varicocele?

In male infertility, the absence of PLCZ1 alone is sufficient to prevent oocyte activation irrespective of the presence of PAWP.
Title: Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.

Phospholipase C-zeta deficiency is associated with repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization.
Title: Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report.

Human PLCzeta elicited the characteristic Ca(2+) oscillations present at mammalian fertilization, which produced oocyte activation and embryo development.
Title: Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.

The following PLCZ1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCZ1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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Up to 50% OFF! Starting
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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu74116 NM_001330769.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu56044 NM_033123.3
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $356.30
$509.00
OHu84306 XM_017020182.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu84307 XM_017020183.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu84308 XM_017020184.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu84309 XM_017020185.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu84310 XM_017020186.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu102335 NM_001330774.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu84302 XM_017020176.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu84303 XM_017020177.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu84304 XM_017020178.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu84305 XM_017020180.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu84305 XM_017020181.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu84305 XM_017020179.1
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu46213 XM_005253519.2
Latest version!
Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu74116
Clone ID Related Accession (Same CDS sequence) NM_001330769.1
Accession Version NM_001330769.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1248bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB038101.1, BC125067.1 and AA812556.1. On Sep 3, 2016 this sequence version replaced XM_011520883.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks three consecutive exons in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter, but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125067.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148093 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGACATC TGACTACCCA GTGGTGCTCT CTTTAGAAAA TCACTGCTCC
ACTGCCCAAC AAGAAGTAAT GGCAGACAAT TTGCAGGCTA CTTTTGGAGA GTCCTTGCTT
TCTGATATGC TTGATGATTT TCCTGATACT CTACCATCAC CAGAGGCACT AAAATTCAAA
ATATTAGTTA AAAATAAGAA AATAGGAACC TTAAAGGAAA CCCATGAAAG AAAAGGTTCT
GATAAGCGTG GAGACAATCA AGACAAGGAA ACAGGGGTAA AAAAGTTACC TGGAGTAATG
CTTTTCAAGA AAAAGAAGAC CAGGAAGCTA AAAATTGCTC TGGCCTTATC TGATCTTGTC
ATTTATACGA AAGCTGAGAA ATTCAAAAGC TTTCAACATT CAAGATTATA TCAGCAATTT
AATGAAAATA ATTCTATTGG GGAGACACAA GCCCGAAAAC TTTCAAAATT GCGAGTCCAT
GAGTTTATTT TTCACACCAG GAAGTTCATT ACCAGAATAT ATCCCAAAGC AACAAGAGCA
GACTCTTCTA ATTTTAATCC CCAAGAATTT TGGAATATAG GTTGTCAAAT GGTGGCTTTA
AATTTCCAGA CCCCTGGTCT GCCCATGGAT CTGCAAAATG GGAAATTTTT GGATAATGGT
GGTTCTGGAT ATATTTTGAA ACCACATTTC TTAAGAGAGA GTAAATCATA CTTTAACCCA
AGTAACATAA AAGAGGGTAT GCCAATTACA CTTACAATAA GGCTCATCAG TGGTATCCAG
TTGCCTCTTA CTCATTCATC ATCTAACAAA GGTGATTCAT TAGTAATTAT AGAAGTTTTT
GGTGTTCCAA ATGATCAAAT GAAGCAGCAG ACTCGTGTAA TTAAAAAAAA TGCTTTTAGT
CCAAGATGGA ATGAAACATT CACATTTATT ATTCATGTCC CAGAATTGGC ATTGATACGT
TTTGTTGTTG AAGGTCAAGG TTTAATAGCA GGAAATGAAT TTCTTGGGCA ATATACTTTG
CCACTTCTAT GCATGAACAA AGGTTATCGT CGTATTCCTC TGTTTTCCAG AATGGGTGAG
AGCCTTGAGC CTGCTTCACT GTTTGTTTAT GTTTGGTACG TCAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317698.1
CDS139..1386
Misc Feature(1)103..105
Misc Feature(2)<241..915
Misc Feature(3)1000..1377
Misc Feature(4)order(1072..1074,1144..1146,1231..1233,1237..1239,1255..1257,1261..1263)
Exon (1)1..149
Exon (2)150..273
Exon (3)274..423
Exon (4)424..508
Exon (5)509..576
Exon (6)577..733
Exon (7)734..850
Exon (8)851..1020
Exon (9)1021..1150
Exon (10)1151..1300
Exon (11)1301..1445
Translation

Target ORF information:

RefSeq Version NM_001330769.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001330769.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGACATC TGACTACCCA GTGGTGCTCT CTTTAGAAAA TCACTGCTCC
ACTGCCCAAC AAGAAGTAAT GGCAGACAAT TTGCAGGCTA CTTTTGGAGA GTCCTTGCTT
TCTGATATGC TTGATGATTT TCCTGATACT CTACCATCAC CAGAGGCACT AAAATTCAAA
ATATTAGTTA AAAATAAGAA AATAGGAACC TTAAAGGAAA CCCATGAAAG AAAAGGTTCT
GATAAGCGTG GAGACAATCA AGACAAGGAA ACAGGGGTAA AAAAGTTACC TGGAGTAATG
CTTTTCAAGA AAAAGAAGAC CAGGAAGCTA AAAATTGCTC TGGCCTTATC TGATCTTGTC
ATTTATACGA AAGCTGAGAA ATTCAAAAGC TTTCAACATT CAAGATTATA TCAGCAATTT
AATGAAAATA ATTCTATTGG GGAGACACAA GCCCGAAAAC TTTCAAAATT GCGAGTCCAT
GAGTTTATTT TTCACACCAG GAAGTTCATT ACCAGAATAT ATCCCAAAGC AACAAGAGCA
GACTCTTCTA ATTTTAATCC CCAAGAATTT TGGAATATAG GTTGTCAAAT GGTGGCTTTA
AATTTCCAGA CCCCTGGTCT GCCCATGGAT CTGCAAAATG GGAAATTTTT GGATAATGGT
GGTTCTGGAT ATATTTTGAA ACCACATTTC TTAAGAGAGA GTAAATCATA CTTTAACCCA
AGTAACATAA AAGAGGGTAT GCCAATTACA CTTACAATAA GGCTCATCAG TGGTATCCAG
TTGCCTCTTA CTCATTCATC ATCTAACAAA GGTGATTCAT TAGTAATTAT AGAAGTTTTT
GGTGTTCCAA ATGATCAAAT GAAGCAGCAG ACTCGTGTAA TTAAAAAAAA TGCTTTTAGT
CCAAGATGGA ATGAAACATT CACATTTATT ATTCATGTCC CAGAATTGGC ATTGATACGT
TTTGTTGTTG AAGGTCAAGG TTTAATAGCA GGAAATGAAT TTCTTGGGCA ATATACTTTG
CCACTTCTAT GCATGAACAA AGGTTATCGT CGTATTCCTC TGTTTTCCAG AATGGGTGAG
AGCCTTGAGC CTGCTTCACT GTTTGTTTAT GTTTGGTACG TCAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56044
Clone ID Related Accession (Same CDS sequence) NM_033123.3
Accession Version NM_033123.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-02
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB038101.1, AF532185.1 and HY197954.1. This sequence is a reference standard in the RefSeqGene project. On Aug 4, 2012 this sequence version replaced NM_033123.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292279.1, AF532185.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2145774 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGACATCT
GACTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCA CTGCCCAACA AGAAGTAATG
GCAGACAATT TGCAGGCTAC TTTTGGAGAG TCCTTGCTTT CTGATATGCT TGATGATTTT
CCTGATACTC TACCATCACC AGAGGCACTA AAATTCAAAA TATTAGTTAA AAATAAGAAA
ATAGGAACCT TAAAGGAAAC CCATGAAAGA AAAGGTTCTG ATAAGCGTGG AGACAATCAA
GACAAGGAAA CAGGGGTAAA AAAGTTACCT GGAGTAATGC TTTTCAAGAA AAAGAAGACC
AGGAAGCTAA AAATTGCTCT GGCCTTATCT GATCTTGTCA TTTATACGAA AGCTGAGAAA
TTCAAAAGCT TTCAACATTC AAGATTATAT CAGCAATTTA ATGAAAATAA TTCTATTGGG
GAGACACAAG CCCGAAAACT TTCAAAATTG CGAGTCCATG AGTTTATTTT TCACACCAGG
AAGTTCATTA CCAGAATATA TCCCAAAGCA ACAAGAGCAG ACTCTTCTAA TTTTAATCCC
CAAGAATTTT GGAATATAGG TTGTCAAATG GTGGCTTTAA ATTTCCAGAC CCCTGGTCTG
CCCATGGATC TGCAAAATGG GAAATTTTTG GATAATGGTG GTTCTGGATA TATTTTGAAA
CCACATTTCT TAAGAGAGAG TAAATCATAC TTTAACCCAA GTAACATAAA AGAGGGTATG
CCAATTACAC TTACAATAAG GCTCATCAGT GGTATCCAGT TGCCTCTTAC TCATTCATCA
TCTAACAAAG GTGATTCATT AGTAATTATA GAAGTTTTTG GTGTTCCAAA TGATCAAATG
AAGCAGCAGA CTCGTGTAAT TAAAAAAAAT GCTTTTAGTC CAAGATGGAA TGAAACATTC
ACATTTATTA TTCATGTCCC AGAATTGGCA TTGATACGTT TTGTTGTTGA AGGTCAAGGT
TTAATAGCAG GAAATGAATT TCTTGGGCAA TATACTTTGC CACTTCTATG CATGAACAAA
GGTTATCGTC GTATTCCTCT GTTTTCCAGA ATGGGTGAGA GCCTTGAGCC TGCTTCACTG
TTTGTTTATG TTTGGTACGT CAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_149114.2
CDS267..2093
Misc Feature(1)231..233
Misc Feature(2)477..2087
Misc Feature(3)480..728
Misc Feature(4)726..1622
Misc Feature(5)order(774..779,864..866,870..872,909..911,1011..1013,1155..1157,1161..1163,1398..1400,1479..1481,1485..1487)
Misc Feature(6)order(774..776,909..911)
Misc Feature(7)order(777..779,864..866,870..872,1011..1013)
Misc Feature(8)1707..2084
Misc Feature(9)order(1779..1781,1851..1853,1938..1940,1944..1946,1962..1964,1968..1970)
Exon (1)1..128
Exon (2)129..277
Exon (3)278..401
Exon (4)402..633
Exon (5)634..835
Exon (6)836..980
Exon (7)981..1130
Exon (8)1131..1215
Exon (9)1216..1283
Exon (10)1284..1440
Exon (11)1441..1557
Exon (12)1558..1727
Exon (13)1728..1857
Exon (14)1858..2007
Exon (15)2008..2156
Translation

Target ORF information:

RefSeq Version NM_033123.3
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033123.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGACATCT
GACTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCA CTGCCCAACA AGAAGTAATG
GCAGACAATT TGCAGGCTAC TTTTGGAGAG TCCTTGCTTT CTGATATGCT TGATGATTTT
CCTGATACTC TACCATCACC AGAGGCACTA AAATTCAAAA TATTAGTTAA AAATAAGAAA
ATAGGAACCT TAAAGGAAAC CCATGAAAGA AAAGGTTCTG ATAAGCGTGG AGACAATCAA
GACAAGGAAA CAGGGGTAAA AAAGTTACCT GGAGTAATGC TTTTCAAGAA AAAGAAGACC
AGGAAGCTAA AAATTGCTCT GGCCTTATCT GATCTTGTCA TTTATACGAA AGCTGAGAAA
TTCAAAAGCT TTCAACATTC AAGATTATAT CAGCAATTTA ATGAAAATAA TTCTATTGGG
GAGACACAAG CCCGAAAACT TTCAAAATTG CGAGTCCATG AGTTTATTTT TCACACCAGG
AAGTTCATTA CCAGAATATA TCCCAAAGCA ACAAGAGCAG ACTCTTCTAA TTTTAATCCC
CAAGAATTTT GGAATATAGG TTGTCAAATG GTGGCTTTAA ATTTCCAGAC CCCTGGTCTG
CCCATGGATC TGCAAAATGG GAAATTTTTG GATAATGGTG GTTCTGGATA TATTTTGAAA
CCACATTTCT TAAGAGAGAG TAAATCATAC TTTAACCCAA GTAACATAAA AGAGGGTATG
CCAATTACAC TTACAATAAG GCTCATCAGT GGTATCCAGT TGCCTCTTAC TCATTCATCA
TCTAACAAAG GTGATTCATT AGTAATTATA GAAGTTTTTG GTGTTCCAAA TGATCAAATG
AAGCAGCAGA CTCGTGTAAT TAAAAAAAAT GCTTTTAGTC CAAGATGGAA TGAAACATTC
ACATTTATTA TTCATGTCCC AGAATTGGCA TTGATACGTT TTGTTGTTGA AGGTCAAGGT
TTAATAGCAG GAAATGAATT TCTTGGGCAA TATACTTTGC CACTTCTATG CATGAACAAA
GGTTATCGTC GTATTCCTCT GTTTTCCAGA ATGGGTGAGA GCCTTGAGCC TGCTTCACTG
TTTGTTTATG TTTGGTACGT CAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84306
Clone ID Related Accession (Same CDS sequence) XM_017020182.1
Accession Version XM_017020182.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGGTGCCC TTGTGAAAGG ATGCCGTTGT TTGGAGATTG ACTGCTGGGA TGGAGCACAA 
AATGAACCTG TTGTATATCA TGGCTACACA CTCACAAGCA AACTTCTGTT TAAAACTGTT
ATCCAAGCTA TACACAAGTA TGCATTCATG ACATCTGACT ACCCAGTGGT GCTCTCTTTA
GAAAATCACT GCTCCACTGC CCAACAAGAA GTAATGGCAG ACAATTTGCA GGCTACTTTT
GGAGAGTCCT TGCTTTCTGA TATGCTTGAT GATTTTCCTG ATACTCTACC ATCACCAGAG
GCACTAAAAT TCAAAATATT AGTTAAAAAT AAGAAAATAG GAACCTTAAA GGAAACCCAT
GAAAGAAAAG GTTCTGATAA GCGTGGTAAG GTGGAGGAAT GGGAAGAAGA AGTGGCAGAT
GGAGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA TAAATTCAAA
GAATCAGAAG TATTGGAATC TGTTTTAGGA GACAATCAAG ACAAGGAAAC AGGGGTAAAA
AAGTTACCTG GAGTAATGCT TTTCAAGAAA AAGAAGACCA GGAAGCTAAA AATTGCTCTG
GCCTTATCTG ATCTTGTCAT TTATACGAAA GCTGAGAAAT TCAAAAGCTT TCAACATTCA
AGATTATATC AGCAATTTAA TGAAAATAAT TCTATTGGGG AGACACAAGC CCGAAAACTT
TCAAAATTGC GAGTCCATGA GTTTATTTTT CACACCAGGA AGTTCATTAC CAGAATATAT
CCCAAAGCAA CAAGAGCAGA CTCTTCTAAT TTTAATCCCC AAGAATTTTG GAATATAGGT
TGTCAAATGG TGGCTTTAAA TTTCCAGACC CCTGGTCTGC CCATGGATCT GCAAAATGGG
AAATTTTTGG ATAATGGTGG TTCTGGATAT ATTTTGAAAC CACATTTCTT AAGAGAGAGT
AAATCATACT TTAACCCAAG TAACATAAAA GAGGGTATGC CAATTACACT TACAATAAGG
CTCATCAGTG GTATCCAGTT GCCTCTTACT CATTCATCAT CTAACAAAGG TGATTCATTA
GTAATTATAG AAGTTTTTGG TGTTCCAAAT GATCAAATGA AGCAGCAGAC TCGTGTAATT
AAAAAAAATG CTTTTAGTCC AAGATGGAAT GAAACATTCA CATTTATTAT TCATGTCCCA
GAATTGGCAT TGATACGTTT TGTTGTTGAA GGTCAAGGTT TAATAGCAGG AAATGAATTT
CTTGGGCAAT ATACTTTGCC ACTTCTATGC ATGAACAAAG GTTATCGTCG TATTCCTCTG
TTTTCCAGAA TGGGTGAGAG CCTTGAGCCT GCTTCACTGT TTGTTTATGT TTGTTTCACA
GAGCATCAAC CCGGACACAG CCACTCTGTA CCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875671.1
CDS477..1892
Translation

Target ORF information:

RefSeq Version XM_017020182.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020182.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGGTGCCC TTGTGAAAGG ATGCCGTTGT TTGGAGATTG ACTGCTGGGA TGGAGCACAA 
AATGAACCTG TTGTATATCA TGGCTACACA CTCACAAGCA AACTTCTGTT TAAAACTGTT
ATCCAAGCTA TACACAAGTA TGCATTCATG ACATCTGACT ACCCAGTGGT GCTCTCTTTA
GAAAATCACT GCTCCACTGC CCAACAAGAA GTAATGGCAG ACAATTTGCA GGCTACTTTT
GGAGAGTCCT TGCTTTCTGA TATGCTTGAT GATTTTCCTG ATACTCTACC ATCACCAGAG
GCACTAAAAT TCAAAATATT AGTTAAAAAT AAGAAAATAG GAACCTTAAA GGAAACCCAT
GAAAGAAAAG GTTCTGATAA GCGTGGTAAG GTGGAGGAAT GGGAAGAAGA AGTGGCAGAT
GGAGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA TAAATTCAAA
GAATCAGAAG TATTGGAATC TGTTTTAGGA GACAATCAAG ACAAGGAAAC AGGGGTAAAA
AAGTTACCTG GAGTAATGCT TTTCAAGAAA AAGAAGACCA GGAAGCTAAA AATTGCTCTG
GCCTTATCTG ATCTTGTCAT TTATACGAAA GCTGAGAAAT TCAAAAGCTT TCAACATTCA
AGATTATATC AGCAATTTAA TGAAAATAAT TCTATTGGGG AGACACAAGC CCGAAAACTT
TCAAAATTGC GAGTCCATGA GTTTATTTTT CACACCAGGA AGTTCATTAC CAGAATATAT
CCCAAAGCAA CAAGAGCAGA CTCTTCTAAT TTTAATCCCC AAGAATTTTG GAATATAGGT
TGTCAAATGG TGGCTTTAAA TTTCCAGACC CCTGGTCTGC CCATGGATCT GCAAAATGGG
AAATTTTTGG ATAATGGTGG TTCTGGATAT ATTTTGAAAC CACATTTCTT AAGAGAGAGT
AAATCATACT TTAACCCAAG TAACATAAAA GAGGGTATGC CAATTACACT TACAATAAGG
CTCATCAGTG GTATCCAGTT GCCTCTTACT CATTCATCAT CTAACAAAGG TGATTCATTA
GTAATTATAG AAGTTTTTGG TGTTCCAAAT GATCAAATGA AGCAGCAGAC TCGTGTAATT
AAAAAAAATG CTTTTAGTCC AAGATGGAAT GAAACATTCA CATTTATTAT TCATGTCCCA
GAATTGGCAT TGATACGTTT TGTTGTTGAA GGTCAAGGTT TAATAGCAGG AAATGAATTT
CTTGGGCAAT ATACTTTGCC ACTTCTATGC ATGAACAAAG GTTATCGTCG TATTCCTCTG
TTTTCCAGAA TGGGTGAGAG CCTTGAGCCT GCTTCACTGT TTGTTTATGT TTGTTTCACA
GAGCATCAAC CCGGACACAG CCACTCTGTA CCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84307
Clone ID Related Accession (Same CDS sequence) XM_017020183.1
Accession Version XM_017020183.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGACATC TGACTACCCA GTGGTGCTCT CTTTAGAAAA TCACTGCTCC
ACTGCCCAAC AAGAAGTAAT GGCAGACAAT TTGCAGGCTA CTTTTGGAGA GTCCTTGCTT
TCTGATATGC TTGATGATTT TCCTGATACT CTACCATCAC CAGAGGCACT AAAATTCAAA
ATATTAGTTA AAAATAAGAA AATAGGAACC TTAAAGGAAA CCCATGAAAG AAAAGGTTCT
GATAAGCGTG GTAAGGTGGA GGAATGGGAA GAAGAAGTGG CAGATGGAGA GGAGGAGGAG
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGATAAAT TCAAAGAATC AGAAGTATTG
GAATCTGTTT TAGGAGACAA TCAAGACAAG GAAACAGGGG TAAAAAAGTT ACCTGGAGTA
ATGCTTTTCA AGAAAAAGAA GACCAGGAAG CTAAAAATTG CTCTGGCCTT ATCTGATCTT
GTCATTTATA CGAAAGCTGA GAAATTCAAA AGCTTTCAAC ATTCAAGATT ATATCAGCAA
TTTAATGAAA ATAATTCTAT TGGGGAGACA CAAGCCCGAA AACTTTCAAA ATTGCGAGTC
CATGAGTTTA TTTTTCACAC CAGGAAGTTC ATTACCAGAA TATATCCCAA AGCAACAAGA
GCAGACTCTT CTAATTTTAA TCCCCAAGAA TTTTGGAATA TAGGTTGTCA AATGGTGGCT
TTAAATTTCC AGACCCCTGG TCTGCCCATG GATCTGCAAA ATGGGAAATT TTTGGATAAT
GGTGGTTCTG GATATATTTT GAAACCACAT TTCTTAAGAG AGAGTAAATC ATACTTTAAC
CCAAGTAACA TAAAAGAGGG TATGCCAATT ACACTTACAA TAAGGCTCAT CAGTGGTATC
CAGTTGCCTC TTACTCATTC ATCATCTAAC AAAGGTGATT CATTAGTAAT TATAGAAGTT
TTTGGTGTTC CAAATGATCA AATGAAGCAG CAGACTCGTG TAATTAAAAA AAATGCTTTT
AGTCCAAGAT GGAATGAAAC ATTCACATTT ATTATTCATG TCCCAGAATT GGCATTGATA
CGTTTTGTTG TTGAAGGTCA AGGTTTAATA GCAGGAAATG AATTTCTTGG GCAATATACT
TTGCCACTTC TATGCATGAA CAAAGGTTAT CGTCGTATTC CTCTGTTTTC CAGAATGGGT
GAGAGCCTTG AGCCTGCTTC ACTGTTTGTT TATGTTTGTT TCACAGAGCA TCAACCCGGA
CACAGCCACT CTGTACCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875672.1
CDS256..1656
Translation

Target ORF information:

RefSeq Version XM_017020183.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020183.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGACATC TGACTACCCA GTGGTGCTCT CTTTAGAAAA TCACTGCTCC
ACTGCCCAAC AAGAAGTAAT GGCAGACAAT TTGCAGGCTA CTTTTGGAGA GTCCTTGCTT
TCTGATATGC TTGATGATTT TCCTGATACT CTACCATCAC CAGAGGCACT AAAATTCAAA
ATATTAGTTA AAAATAAGAA AATAGGAACC TTAAAGGAAA CCCATGAAAG AAAAGGTTCT
GATAAGCGTG GTAAGGTGGA GGAATGGGAA GAAGAAGTGG CAGATGGAGA GGAGGAGGAG
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGATAAAT TCAAAGAATC AGAAGTATTG
GAATCTGTTT TAGGAGACAA TCAAGACAAG GAAACAGGGG TAAAAAAGTT ACCTGGAGTA
ATGCTTTTCA AGAAAAAGAA GACCAGGAAG CTAAAAATTG CTCTGGCCTT ATCTGATCTT
GTCATTTATA CGAAAGCTGA GAAATTCAAA AGCTTTCAAC ATTCAAGATT ATATCAGCAA
TTTAATGAAA ATAATTCTAT TGGGGAGACA CAAGCCCGAA AACTTTCAAA ATTGCGAGTC
CATGAGTTTA TTTTTCACAC CAGGAAGTTC ATTACCAGAA TATATCCCAA AGCAACAAGA
GCAGACTCTT CTAATTTTAA TCCCCAAGAA TTTTGGAATA TAGGTTGTCA AATGGTGGCT
TTAAATTTCC AGACCCCTGG TCTGCCCATG GATCTGCAAA ATGGGAAATT TTTGGATAAT
GGTGGTTCTG GATATATTTT GAAACCACAT TTCTTAAGAG AGAGTAAATC ATACTTTAAC
CCAAGTAACA TAAAAGAGGG TATGCCAATT ACACTTACAA TAAGGCTCAT CAGTGGTATC
CAGTTGCCTC TTACTCATTC ATCATCTAAC AAAGGTGATT CATTAGTAAT TATAGAAGTT
TTTGGTGTTC CAAATGATCA AATGAAGCAG CAGACTCGTG TAATTAAAAA AAATGCTTTT
AGTCCAAGAT GGAATGAAAC ATTCACATTT ATTATTCATG TCCCAGAATT GGCATTGATA
CGTTTTGTTG TTGAAGGTCA AGGTTTAATA GCAGGAAATG AATTTCTTGG GCAATATACT
TTGCCACTTC TATGCATGAA CAAAGGTTAT CGTCGTATTC CTCTGTTTTC CAGAATGGGT
GAGAGCCTTG AGCCTGCTTC ACTGTTTGTT TATGTTTGTT TCACAGAGCA TCAACCCGGA
CACAGCCACT CTGTACCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84308
Clone ID Related Accession (Same CDS sequence) XM_017020184.1
Accession Version XM_017020184.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACATCTG ACTACCCAGT GGTGCTCTCT TTAGAAAATC ACTGCTCCAC TGCCCAACAA 
GAAGTAATGG CAGACAATTT GCAGGCTACT TTTGGAGAGT CCTTGCTTTC TGATATGCTT
GATGATTTTC CTGATACTCT ACCATCACCA GAGGCACTAA AATTCAAAAT ATTAGTTAAA
AATAAGAAAA TAGGAACCTT AAAGGAAACC CATGAAAGAA AAGGTTCTGA TAAGCGTGGT
AAGGTGGAGG AATGGGAAGA AGAAGTGGCA GATGGAGAGG AGGAGGAGGA GGAGGAGGAG
GAGGAGGAGG AGGAGGAGGA GGATAAATTC AAAGAATCAG AAGTATTGGA ATCTGTTTTA
GGAGACAATC AAGACAAGGA AACAGGGGTA AAAAAGTTAC CTGGAGTAAT GCTTTTCAAG
AAAAAGAAGA CCAGGAAGCT AAAAATTGCT CTGGCCTTAT CTGATCTTGT CATTTATACG
AAAGCTGAGA AATTCAAAAG CTTTCAACAT TCAAGATTAT ATCAGCAATT TAATGAAAAT
AATTCTATTG GGGAGACACA AGCCCGAAAA CTTTCAAAAT TGCGAGTCCA TGAGTTTATT
TTTCACACCA GGAAGTTCAT TACCAGAATA TATCCCAAAG CAACAAGAGC AGACTCTTCT
AATTTTAATC CCCAAGAATT TTGGAATATA GGTTGTCAAA TGGTGGCTTT AAATTTCCAG
ACCCCTGGTC TGCCCATGGA TCTGCAAAAT GGGAAATTTT TGGATAATGG TGGTTCTGGA
TATATTTTGA AACCACATTT CTTAAGAGAG AGTAAATCAT ACTTTAACCC AAGTAACATA
AAAGAGGGTA TGCCAATTAC ACTTACAATA AGGCTCATCA GTGGTATCCA GTTGCCTCTT
ACTCATTCAT CATCTAACAA AGGTGATTCA TTAGTAATTA TAGAAGTTTT TGGTGTTCCA
AATGATCAAA TGAAGCAGCA GACTCGTGTA ATTAAAAAAA ATGCTTTTAG TCCAAGATGG
AATGAAACAT TCACATTTAT TATTCATGTC CCAGAATTGG CATTGATACG TTTTGTTGTT
GAAGGTCAAG GTTTAATAGC AGGAAATGAA TTTCTTGGGC AATATACTTT GCCACTTCTA
TGCATGAACA AAGGTTATCG TCGTATTCCT CTGTTTTCCA GAATGGGTGA GAGCCTTGAG
CCTGCTTCAC TGTTTGTTTA TGTTTGTTTC ACAGAGCATC AACCCGGACA CAGCCACTCT
GTACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875673.1
CDS316..1584
Translation

Target ORF information:

RefSeq Version XM_017020184.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020184.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACATCTG ACTACCCAGT GGTGCTCTCT TTAGAAAATC ACTGCTCCAC TGCCCAACAA 
GAAGTAATGG CAGACAATTT GCAGGCTACT TTTGGAGAGT CCTTGCTTTC TGATATGCTT
GATGATTTTC CTGATACTCT ACCATCACCA GAGGCACTAA AATTCAAAAT ATTAGTTAAA
AATAAGAAAA TAGGAACCTT AAAGGAAACC CATGAAAGAA AAGGTTCTGA TAAGCGTGGT
AAGGTGGAGG AATGGGAAGA AGAAGTGGCA GATGGAGAGG AGGAGGAGGA GGAGGAGGAG
GAGGAGGAGG AGGAGGAGGA GGATAAATTC AAAGAATCAG AAGTATTGGA ATCTGTTTTA
GGAGACAATC AAGACAAGGA AACAGGGGTA AAAAAGTTAC CTGGAGTAAT GCTTTTCAAG
AAAAAGAAGA CCAGGAAGCT AAAAATTGCT CTGGCCTTAT CTGATCTTGT CATTTATACG
AAAGCTGAGA AATTCAAAAG CTTTCAACAT TCAAGATTAT ATCAGCAATT TAATGAAAAT
AATTCTATTG GGGAGACACA AGCCCGAAAA CTTTCAAAAT TGCGAGTCCA TGAGTTTATT
TTTCACACCA GGAAGTTCAT TACCAGAATA TATCCCAAAG CAACAAGAGC AGACTCTTCT
AATTTTAATC CCCAAGAATT TTGGAATATA GGTTGTCAAA TGGTGGCTTT AAATTTCCAG
ACCCCTGGTC TGCCCATGGA TCTGCAAAAT GGGAAATTTT TGGATAATGG TGGTTCTGGA
TATATTTTGA AACCACATTT CTTAAGAGAG AGTAAATCAT ACTTTAACCC AAGTAACATA
AAAGAGGGTA TGCCAATTAC ACTTACAATA AGGCTCATCA GTGGTATCCA GTTGCCTCTT
ACTCATTCAT CATCTAACAA AGGTGATTCA TTAGTAATTA TAGAAGTTTT TGGTGTTCCA
AATGATCAAA TGAAGCAGCA GACTCGTGTA ATTAAAAAAA ATGCTTTTAG TCCAAGATGG
AATGAAACAT TCACATTTAT TATTCATGTC CCAGAATTGG CATTGATACG TTTTGTTGTT
GAAGGTCAAG GTTTAATAGC AGGAAATGAA TTTCTTGGGC AATATACTTT GCCACTTCTA
TGCATGAACA AAGGTTATCG TCGTATTCCT CTGTTTTCCA GAATGGGTGA GAGCCTTGAG
CCTGCTTCAC TGTTTGTTTA TGTTTGTTTC ACAGAGCATC AACCCGGACA CAGCCACTCT
GTACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84309
Clone ID Related Accession (Same CDS sequence) XM_017020185.1
Accession Version XM_017020185.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCAGACA ATTTGCAGGC TACTTTTGGA GAGTCCTTGC TTTCTGATAT GCTTGATGAT 
TTTCCTGATA CTCTACCATC ACCAGAGGCA CTAAAATTCA AAATATTAGT TAAAAATAAG
AAAATAGGAA CCTTAAAGGA AACCCATGAA AGAAAAGGTT CTGATAAGCG TGGTAAGGTG
GAGGAATGGG AAGAAGAAGT GGCAGATGGA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GAGGAGGAGG AGGAGGATAA ATTCAAAGAA TCAGAAGTAT TGGAATCTGT TTTAGGAGAC
AATCAAGACA AGGAAACAGG GGTAAAAAAG TTACCTGGAG TAATGCTTTT CAAGAAAAAG
AAGACCAGGA AGCTAAAAAT TGCTCTGGCC TTATCTGATC TTGTCATTTA TACGAAAGCT
GAGAAATTCA AAAGCTTTCA ACATTCAAGA TTATATCAGC AATTTAATGA AAATAATTCT
ATTGGGGAGA CACAAGCCCG AAAACTTTCA AAATTGCGAG TCCATGAGTT TATTTTTCAC
ACCAGGAAGT TCATTACCAG AATATATCCC AAAGCAACAA GAGCAGACTC TTCTAATTTT
AATCCCCAAG AATTTTGGAA TATAGGTTGT CAAATGGTGG CTTTAAATTT CCAGACCCCT
GGTCTGCCCA TGGATCTGCA AAATGGGAAA TTTTTGGATA ATGGTGGTTC TGGATATATT
TTGAAACCAC ATTTCTTAAG AGAGAGTAAA TCATACTTTA ACCCAAGTAA CATAAAAGAG
GGTATGCCAA TTACACTTAC AATAAGGCTC ATCAGTGGTA TCCAGTTGCC TCTTACTCAT
TCATCATCTA ACAAAGGTGA TTCATTAGTA ATTATAGAAG TTTTTGGTGT TCCAAATGAT
CAAATGAAGC AGCAGACTCG TGTAATTAAA AAAAATGCTT TTAGTCCAAG ATGGAATGAA
ACATTCACAT TTATTATTCA TGTCCCAGAA TTGGCATTGA TACGTTTTGT TGTTGAAGGT
CAAGGTTTAA TAGCAGGAAA TGAATTTCTT GGGCAATATA CTTTGCCACT TCTATGCATG
AACAAAGGTT ATCGTCGTAT TCCTCTGTTT TCCAGAATGG GTGAGAGCCT TGAGCCTGCT
TCACTGTTTG TTTATGTTTG TTTCACAGAG CATCAACCCG GACACAGCCA CTCTGTACCA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875674.1
CDS267..1469
Translation

Target ORF information:

RefSeq Version XM_017020185.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020185.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCAGACA ATTTGCAGGC TACTTTTGGA GAGTCCTTGC TTTCTGATAT GCTTGATGAT 
TTTCCTGATA CTCTACCATC ACCAGAGGCA CTAAAATTCA AAATATTAGT TAAAAATAAG
AAAATAGGAA CCTTAAAGGA AACCCATGAA AGAAAAGGTT CTGATAAGCG TGGTAAGGTG
GAGGAATGGG AAGAAGAAGT GGCAGATGGA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GAGGAGGAGG AGGAGGATAA ATTCAAAGAA TCAGAAGTAT TGGAATCTGT TTTAGGAGAC
AATCAAGACA AGGAAACAGG GGTAAAAAAG TTACCTGGAG TAATGCTTTT CAAGAAAAAG
AAGACCAGGA AGCTAAAAAT TGCTCTGGCC TTATCTGATC TTGTCATTTA TACGAAAGCT
GAGAAATTCA AAAGCTTTCA ACATTCAAGA TTATATCAGC AATTTAATGA AAATAATTCT
ATTGGGGAGA CACAAGCCCG AAAACTTTCA AAATTGCGAG TCCATGAGTT TATTTTTCAC
ACCAGGAAGT TCATTACCAG AATATATCCC AAAGCAACAA GAGCAGACTC TTCTAATTTT
AATCCCCAAG AATTTTGGAA TATAGGTTGT CAAATGGTGG CTTTAAATTT CCAGACCCCT
GGTCTGCCCA TGGATCTGCA AAATGGGAAA TTTTTGGATA ATGGTGGTTC TGGATATATT
TTGAAACCAC ATTTCTTAAG AGAGAGTAAA TCATACTTTA ACCCAAGTAA CATAAAAGAG
GGTATGCCAA TTACACTTAC AATAAGGCTC ATCAGTGGTA TCCAGTTGCC TCTTACTCAT
TCATCATCTA ACAAAGGTGA TTCATTAGTA ATTATAGAAG TTTTTGGTGT TCCAAATGAT
CAAATGAAGC AGCAGACTCG TGTAATTAAA AAAAATGCTT TTAGTCCAAG ATGGAATGAA
ACATTCACAT TTATTATTCA TGTCCCAGAA TTGGCATTGA TACGTTTTGT TGTTGAAGGT
CAAGGTTTAA TAGCAGGAAA TGAATTTCTT GGGCAATATA CTTTGCCACT TCTATGCATG
AACAAAGGTT ATCGTCGTAT TCCTCTGTTT TCCAGAATGG GTGAGAGCCT TGAGCCTGCT
TCACTGTTTG TTTATGTTTG TTTCACAGAG CATCAACCCG GACACAGCCA CTCTGTACCA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84310
Clone ID Related Accession (Same CDS sequence) XM_017020186.1
Accession Version XM_017020186.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCAGACA ATTTGCAGGC TACTTTTGGA GAGTCCTTGC TTTCTGATAT GCTTGATGAT 
TTTCCTGATA CTCTACCATC ACCAGAGGCA CTAAAATTCA AAATATTAGT TAAAAATAAG
AAAATAGGAA CCTTAAAGGA AACCCATGAA AGAAAAGGTT CTGATAAGCG TGGAGACAAT
CAAGACAAGG AAACAGGGGT AAAAAAGTTA CCTGGAGTAA TGCTTTTCAA GAAAAAGAAG
ACCAGGAAGC TAAAAATTGC TCTGGCCTTA TCTGATCTTG TCATTTATAC GAAAGCTGAG
AAATTCAAAA GCTTTCAACA TTCAAGATTA TATCAGCAAT TTAATGAAAA TAATTCTATT
GGGGAGACAC AAGCCCGAAA ACTTTCAAAA TTGCGAGTCC ATGAGTTTAT TTTTCACACC
AGGAAGTTCA TTACCAGAAT ATATCCCAAA GCAACAAGAG CAGACTCTTC TAATTTTAAT
CCCCAAGAAT TTTGGAATAT AGGTTGTCAA ATGGTGGCTT TAAATTTCCA GACCCCTGGT
CTGCCCATGG ATCTGCAAAA TGGGAAATTT TTGGATAATG GTGGTTCTGG ATATATTTTG
AAACCACATT TCTTAAGAGA GAGTAAATCA TACTTTAACC CAAGTAACAT AAAAGAGGGT
ATGCCAATTA CACTTACAAT AAGGCTCATC AGTGGTATCC AGTTGCCTCT TACTCATTCA
TCATCTAACA AAGGTGATTC ATTAGTAATT ATAGAAGTTT TTGGTGTTCC AAATGATCAA
ATGAAGCAGC AGACTCGTGT AATTAAAAAA AATGCTTTTA GTCCAAGATG GAATGAAACA
TTCACATTTA TTATTCATGT CCCAGAATTG GCATTGATAC GTTTTGTTGT TGAAGGTCAA
GGTTTAATAG CAGGAAATGA ATTTCTTGGG CAATATACTT TGCCACTTCT ATGCATGAAC
AAAGGTTATC GTCGTATTCC TCTGTTTTCC AGAATGGGTG AGAGCCTTGA GCCTGCTTCA
CTGTTTGTTT ATGTTTGTTT CACAGAGCAT CAACCCGGAC ACAGCCACTC TGTACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875675.1
CDS412..1491
Translation

Target ORF information:

RefSeq Version XM_017020186.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020186.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCAGACA ATTTGCAGGC TACTTTTGGA GAGTCCTTGC TTTCTGATAT GCTTGATGAT 
TTTCCTGATA CTCTACCATC ACCAGAGGCA CTAAAATTCA AAATATTAGT TAAAAATAAG
AAAATAGGAA CCTTAAAGGA AACCCATGAA AGAAAAGGTT CTGATAAGCG TGGAGACAAT
CAAGACAAGG AAACAGGGGT AAAAAAGTTA CCTGGAGTAA TGCTTTTCAA GAAAAAGAAG
ACCAGGAAGC TAAAAATTGC TCTGGCCTTA TCTGATCTTG TCATTTATAC GAAAGCTGAG
AAATTCAAAA GCTTTCAACA TTCAAGATTA TATCAGCAAT TTAATGAAAA TAATTCTATT
GGGGAGACAC AAGCCCGAAA ACTTTCAAAA TTGCGAGTCC ATGAGTTTAT TTTTCACACC
AGGAAGTTCA TTACCAGAAT ATATCCCAAA GCAACAAGAG CAGACTCTTC TAATTTTAAT
CCCCAAGAAT TTTGGAATAT AGGTTGTCAA ATGGTGGCTT TAAATTTCCA GACCCCTGGT
CTGCCCATGG ATCTGCAAAA TGGGAAATTT TTGGATAATG GTGGTTCTGG ATATATTTTG
AAACCACATT TCTTAAGAGA GAGTAAATCA TACTTTAACC CAAGTAACAT AAAAGAGGGT
ATGCCAATTA CACTTACAAT AAGGCTCATC AGTGGTATCC AGTTGCCTCT TACTCATTCA
TCATCTAACA AAGGTGATTC ATTAGTAATT ATAGAAGTTT TTGGTGTTCC AAATGATCAA
ATGAAGCAGC AGACTCGTGT AATTAAAAAA AATGCTTTTA GTCCAAGATG GAATGAAACA
TTCACATTTA TTATTCATGT CCCAGAATTG GCATTGATAC GTTTTGTTGT TGAAGGTCAA
GGTTTAATAG CAGGAAATGA ATTTCTTGGG CAATATACTT TGCCACTTCT ATGCATGAAC
AAAGGTTATC GTCGTATTCC TCTGTTTTCC AGAATGGGTG AGAGCCTTGA GCCTGCTTCA
CTGTTTGTTT ATGTTTGTTT CACAGAGCAT CAACCCGGAC ACAGCCACTC TGTACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102335
Clone ID Related Accession (Same CDS sequence) NM_001330774.1
Accession Version NM_001330774.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1515bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB038101.1, AK314516.1, BC125067.1 and AA812556.1. On Sep 3, 2016 this sequence version replaced NR_073075.1. Transcript Variant: This variant (2) uses an alternate splice site compared to variant 1. This variant represents translation initiation at a downstream start codon compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream start codon to encode an isoform (2) that has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY035866.1, AK314516.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2145774 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTAAAG CTATTGCTTT TGAGATCATT CAGAAATACG AGCCTATCGA AGAAGTTAGG 
AAAGCACACC AAATGTCATT AGAAGGTTTT ACAAGATACA TGGATTCACG TGAATGTCTA
CTGTTTAAAA ATGAATGTAG AAAAGTTTAT CAAGATATGA CTCATCCATT AAATGATTAT
TTTATTTCAT CTTCACATAA CACATATTTG GTATCTGATC AATTATTGGG ACCAAGTGAC
CTTTGGGGAT ATGTAAGTGC CCTTGTGAAA GGATGCCGTT GTTTGGAGAT TGACTGCTGG
GATGGAGCAC AAAATGAACC TGTTGTATAT CATGGCTACA CACTCACAAG CAAACTTCTG
TTTAAAACTG TTATCCAAGC TATACACAAG TATGCATTCA TGACATCTGA CTACCCAGTG
GTGCTCTCTT TAGAAAATCA CTGCTCCACT GCCCAACAAG AAGTAATGGC AGACAATTTG
CAGGCTACTT TTGGAGAGTC CTTGCTTTCT GATATGCTTG ATGATTTTCC TGATACTCTA
CCATCACCAG AGGCACTAAA ATTCAAAATA TTAGTTAAAA ATAAGAAAAT AGGAACCTTA
AAGGAAACCC ATGAAAGAAA AGGTTCTGAT AAGCGTGGAG ACAATCAAGA CAAGGAAACA
GGGGTAAAAA AGTTACCTGG AGTAATGCTT TTCAAGAAAA AGAAGACCAG GAAGCTAAAA
ATTGCTCTGG CCTTATCTGA TCTTGTCATT TATACGAAAG CTGAGAAATT CAAAAGCTTT
CAACATTCAA GATTATATCA GCAATTTAAT GAAAATAATT CTATTGGGGA GACACAAGCC
CGAAAACTTT CAAAATTGCG AGTCCATGAG TTTATTTTTC ACACCAGGAA GTTCATTACC
AGAATATATC CCAAAGCAAC AAGAGCAGAC TCTTCTAATT TTAATCCCCA AGAATTTTGG
AATATAGGTT GTCAAATGGT GGCTTTAAAT TTCCAGACCC CTGGTCTGCC CATGGATCTG
CAAAATGGGA AATTTTTGGA TAATGGTGGT TCTGGATATA TTTTGAAACC ACATTTCTTA
AGAGAGAGTA AATCATACTT TAACCCAAGT AACATAAAAG AGGGTATGCC AATTACACTT
ACAATAAGGC TCATCAGTGG TATCCAGTTG CCTCTTACTC ATTCATCATC TAACAAAGGT
GATTCATTAG TAATTATAGA AGTTTTTGGT GTTCCAAATG ATCAAATGAA GCAGCAGACT
CGTGTAATTA AAAAAAATGC TTTTAGTCCA AGATGGAATG AAACATTCAC ATTTATTATT
CATGTCCCAG AATTGGCATT GATACGTTTT GTTGTTGAAG GTCAAGGTTT AATAGCAGGA
AATGAATTTC TTGGGCAATA TACTTTGCCA CTTCTATGCA TGAACAAAGG TTATCGTCGT
ATTCCTCTGT TTTCCAGAAT GGGTGAGAGC CTTGAGCCTG CTTCACTGTT TGTTTATGTT
TGGTACGTCA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317703.1
CDS582..2096
Misc Feature(1)402..404
Exon (1)1..128
Exon (2)129..277
Exon (3)278..401
Exon (4)402..636
Exon (5)637..838
Exon (6)839..983
Exon (7)984..1133
Exon (8)1134..1218
Exon (9)1219..1286
Exon (10)1287..1443
Exon (11)1444..1560
Exon (12)1561..1730
Exon (13)1731..1860
Exon (14)1861..2010
Exon (15)2011..2155
Translation

Target ORF information:

RefSeq Version NM_001330774.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001330774.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTAAAG CTATTGCTTT TGAGATCATT CAGAAATACG AGCCTATCGA AGAAGTTAGG 
AAAGCACACC AAATGTCATT AGAAGGTTTT ACAAGATACA TGGATTCACG TGAATGTCTA
CTGTTTAAAA ATGAATGTAG AAAAGTTTAT CAAGATATGA CTCATCCATT AAATGATTAT
TTTATTTCAT CTTCACATAA CACATATTTG GTATCTGATC AATTATTGGG ACCAAGTGAC
CTTTGGGGAT ATGTAAGTGC CCTTGTGAAA GGATGCCGTT GTTTGGAGAT TGACTGCTGG
GATGGAGCAC AAAATGAACC TGTTGTATAT CATGGCTACA CACTCACAAG CAAACTTCTG
TTTAAAACTG TTATCCAAGC TATACACAAG TATGCATTCA TGACATCTGA CTACCCAGTG
GTGCTCTCTT TAGAAAATCA CTGCTCCACT GCCCAACAAG AAGTAATGGC AGACAATTTG
CAGGCTACTT TTGGAGAGTC CTTGCTTTCT GATATGCTTG ATGATTTTCC TGATACTCTA
CCATCACCAG AGGCACTAAA ATTCAAAATA TTAGTTAAAA ATAAGAAAAT AGGAACCTTA
AAGGAAACCC ATGAAAGAAA AGGTTCTGAT AAGCGTGGAG ACAATCAAGA CAAGGAAACA
GGGGTAAAAA AGTTACCTGG AGTAATGCTT TTCAAGAAAA AGAAGACCAG GAAGCTAAAA
ATTGCTCTGG CCTTATCTGA TCTTGTCATT TATACGAAAG CTGAGAAATT CAAAAGCTTT
CAACATTCAA GATTATATCA GCAATTTAAT GAAAATAATT CTATTGGGGA GACACAAGCC
CGAAAACTTT CAAAATTGCG AGTCCATGAG TTTATTTTTC ACACCAGGAA GTTCATTACC
AGAATATATC CCAAAGCAAC AAGAGCAGAC TCTTCTAATT TTAATCCCCA AGAATTTTGG
AATATAGGTT GTCAAATGGT GGCTTTAAAT TTCCAGACCC CTGGTCTGCC CATGGATCTG
CAAAATGGGA AATTTTTGGA TAATGGTGGT TCTGGATATA TTTTGAAACC ACATTTCTTA
AGAGAGAGTA AATCATACTT TAACCCAAGT AACATAAAAG AGGGTATGCC AATTACACTT
ACAATAAGGC TCATCAGTGG TATCCAGTTG CCTCTTACTC ATTCATCATC TAACAAAGGT
GATTCATTAG TAATTATAGA AGTTTTTGGT GTTCCAAATG ATCAAATGAA GCAGCAGACT
CGTGTAATTA AAAAAAATGC TTTTAGTCCA AGATGGAATG AAACATTCAC ATTTATTATT
CATGTCCCAG AATTGGCATT GATACGTTTT GTTGTTGAAG GTCAAGGTTT AATAGCAGGA
AATGAATTTC TTGGGCAATA TACTTTGCCA CTTCTATGCA TGAACAAAGG TTATCGTCGT
ATTCCTCTGT TTTCCAGAAT GGGTGAGAGC CTTGAGCCTG CTTCACTGTT TGTTTATGTT
TGGTACGTCA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84302
Clone ID Related Accession (Same CDS sequence) XM_017020176.1
Accession Version XM_017020176.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGACATCT
GACTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCA CTGCCCAACA AGAAGTAATG
GCAGACAATT TGCAGGCTAC TTTTGGAGAG TCCTTGCTTT CTGATATGCT TGATGATTTT
CCTGATACTC TACCATCACC AGAGGCACTA AAATTCAAAA TATTAGTTAA AAATAAGAAA
ATAGGAACCT TAAAGGAAAC CCATGAAAGA AAAGGTTCTG ATAAGCGTGG TAAGGTGGAG
GAATGGGAAG AAGAAGTGGC AGATGGAGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GAGGAGGAGG AGGATAAATT CAAAGAATCA GAAGTATTGG AATCTGTTTT AGGAGACAAT
CAAGACAAGG AAACAGGGGT AAAAAAGTTA CCTGGAGTAA TGCTTTTCAA GAAAAAGAAG
ACCAGGAAGC TAAAAATTGC TCTGGCCTTA TCTGATCTTG TCATTTATAC GAAAGCTGAG
AAATTCAAAA GCTTTCAACA TTCAAGATTA TATCAGCAAT TTAATGAAAA TAATTCTATT
GGGGAGACAC AAGCCCGAAA ACTTTCAAAA TTGCGAGTCC ATGAGTTTAT TTTTCACACC
AGGAAGTTCA TTACCAGAAT ATATCCCAAA GCAACAAGAG CAGACTCTTC TAATTTTAAT
CCCCAAGAAT TTTGGAATAT AGGTTGTCAA ATGGTGGCTT TAAATTTCCA GACCCCTGGT
CTGCCCATGG ATCTGCAAAA TGGGAAATTT TTGGATAATG GTGGTTCTGG ATATATTTTG
AAACCACATT TCTTAAGAGA GAGTAAATCA TACTTTAACC CAAGTAACAT AAAAGAGGGT
ATGCCAATTA CACTTACAAT AAGGCTCATC AGTGGTATCC AGTTGCCTCT TACTCATTCA
TCATCTAACA AAGGTGATTC ATTAGTAATT ATAGAAGTTT TTGGTGTTCC AAATGATCAA
ATGAAGCAGC AGACTCGTGT AATTAAAAAA AATGCTTTTA GTCCAAGATG GAATGAAACA
TTCACATTTA TTATTCATGT CCCAGAATTG GCATTGATAC GTTTTGTTGT TGAAGGTCAA
GGTTTAATAG CAGGAAATGA ATTTCTTGGG CAATATACTT TGCCACTTCT ATGCATGAAC
AAAGGTTATC GTCGTATTCC TCTGTTTTCC AGAATGGGTG AGAGCCTTGA GCCTGCTTCA
CTGTTTGTTT ATGTTTGTTT CACAGAGCAT CAACCCGGAC ACAGCCACTC TGTACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875665.1
CDS260..2239
Translation

Target ORF information:

RefSeq Version XM_017020176.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020176.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGACATCT
GACTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCA CTGCCCAACA AGAAGTAATG
GCAGACAATT TGCAGGCTAC TTTTGGAGAG TCCTTGCTTT CTGATATGCT TGATGATTTT
CCTGATACTC TACCATCACC AGAGGCACTA AAATTCAAAA TATTAGTTAA AAATAAGAAA
ATAGGAACCT TAAAGGAAAC CCATGAAAGA AAAGGTTCTG ATAAGCGTGG TAAGGTGGAG
GAATGGGAAG AAGAAGTGGC AGATGGAGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GAGGAGGAGG AGGATAAATT CAAAGAATCA GAAGTATTGG AATCTGTTTT AGGAGACAAT
CAAGACAAGG AAACAGGGGT AAAAAAGTTA CCTGGAGTAA TGCTTTTCAA GAAAAAGAAG
ACCAGGAAGC TAAAAATTGC TCTGGCCTTA TCTGATCTTG TCATTTATAC GAAAGCTGAG
AAATTCAAAA GCTTTCAACA TTCAAGATTA TATCAGCAAT TTAATGAAAA TAATTCTATT
GGGGAGACAC AAGCCCGAAA ACTTTCAAAA TTGCGAGTCC ATGAGTTTAT TTTTCACACC
AGGAAGTTCA TTACCAGAAT ATATCCCAAA GCAACAAGAG CAGACTCTTC TAATTTTAAT
CCCCAAGAAT TTTGGAATAT AGGTTGTCAA ATGGTGGCTT TAAATTTCCA GACCCCTGGT
CTGCCCATGG ATCTGCAAAA TGGGAAATTT TTGGATAATG GTGGTTCTGG ATATATTTTG
AAACCACATT TCTTAAGAGA GAGTAAATCA TACTTTAACC CAAGTAACAT AAAAGAGGGT
ATGCCAATTA CACTTACAAT AAGGCTCATC AGTGGTATCC AGTTGCCTCT TACTCATTCA
TCATCTAACA AAGGTGATTC ATTAGTAATT ATAGAAGTTT TTGGTGTTCC AAATGATCAA
ATGAAGCAGC AGACTCGTGT AATTAAAAAA AATGCTTTTA GTCCAAGATG GAATGAAACA
TTCACATTTA TTATTCATGT CCCAGAATTG GCATTGATAC GTTTTGTTGT TGAAGGTCAA
GGTTTAATAG CAGGAAATGA ATTTCTTGGG CAATATACTT TGCCACTTCT ATGCATGAAC
AAAGGTTATC GTCGTATTCC TCTGTTTTCC AGAATGGGTG AGAGCCTTGA GCCTGCTTCA
CTGTTTGTTT ATGTTTGTTT CACAGAGCAT CAACCCGGAC ACAGCCACTC TGTACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84303
Clone ID Related Accession (Same CDS sequence) XM_017020177.1
Accession Version XM_017020177.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGACATCT
GACTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCA CTGCCCAACA AGAAGTAATG
GCAGACAATT TGCAGGCTAC TTTTGGAGAG TCCTTGCTTT CTGATATGCT TGATGATTTT
CCTGATACTC TACCATCACC AGAGGCACTA AAATTCAAAA TATTAGTTAA AAATAAGAAA
ATAGGAACCT TAAAGGAAAC CCATGAAAGA AAAGGTTCTG ATAAGCGTGG AGACAATCAA
GACAAGGAAA CAGGGGTAAA AAAGTTACCT GGAGTAATGC TTTTCAAGAA AAAGAAGACC
AGGAAGCTAA AAATTGCTCT GGCCTTATCT GATCTTGTCA TTTATACGAA AGCTGAGAAA
TTCAAAAGCT TTCAACATTC AAGATTATAT CAGCAATTTA ATGAAAATAA TTCTATTGGG
GAGACACAAG CCCGAAAACT TTCAAAATTG CGAGTCCATG AGTTTATTTT TCACACCAGG
AAGTTCATTA CCAGAATATA TCCCAAAGCA ACAAGAGCAG ACTCTTCTAA TTTTAATCCC
CAAGAATTTT GGAATATAGG TTGTCAAATG GTGGCTTTAA ATTTCCAGAC CCCTGGTCTG
CCCATGGATC TGCAAAATGG GAAATTTTTG GATAATGGTG GTTCTGGATA TATTTTGAAA
CCACATTTCT TAAGAGAGAG TAAATCATAC TTTAACCCAA GTAACATAAA AGAGGGTATG
CCAATTACAC TTACAATAAG GCTCATCAGT GGTATCCAGT TGCCTCTTAC TCATTCATCA
TCTAACAAAG GTGATTCATT AGTAATTATA GAAGTTTTTG GTGTTCCAAA TGATCAAATG
AAGCAGCAGA CTCGTGTAAT TAAAAAAAAT GCTTTTAGTC CAAGATGGAA TGAAACATTC
ACATTTATTA TTCATGTCCC AGAATTGGCA TTGATACGTT TTGTTGTTGA AGGTCAAGGT
TTAATAGCAG GAAATGAATT TCTTGGGCAA TATACTTTGC CACTTCTATG CATGAACAAA
GGTTATCGTC GTATTCCTCT GTTTTCCAGA ATGGGTGAGA GCCTTGAGCC TGCTTCACTG
TTTGTTTATG TTTGTTTCAC AGAGCATCAA CCCGGACACA GCCACTCTGT ACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875666.1
CDS260..2116
Translation

Target ORF information:

RefSeq Version XM_017020177.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020177.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGACATCT
GACTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCA CTGCCCAACA AGAAGTAATG
GCAGACAATT TGCAGGCTAC TTTTGGAGAG TCCTTGCTTT CTGATATGCT TGATGATTTT
CCTGATACTC TACCATCACC AGAGGCACTA AAATTCAAAA TATTAGTTAA AAATAAGAAA
ATAGGAACCT TAAAGGAAAC CCATGAAAGA AAAGGTTCTG ATAAGCGTGG AGACAATCAA
GACAAGGAAA CAGGGGTAAA AAAGTTACCT GGAGTAATGC TTTTCAAGAA AAAGAAGACC
AGGAAGCTAA AAATTGCTCT GGCCTTATCT GATCTTGTCA TTTATACGAA AGCTGAGAAA
TTCAAAAGCT TTCAACATTC AAGATTATAT CAGCAATTTA ATGAAAATAA TTCTATTGGG
GAGACACAAG CCCGAAAACT TTCAAAATTG CGAGTCCATG AGTTTATTTT TCACACCAGG
AAGTTCATTA CCAGAATATA TCCCAAAGCA ACAAGAGCAG ACTCTTCTAA TTTTAATCCC
CAAGAATTTT GGAATATAGG TTGTCAAATG GTGGCTTTAA ATTTCCAGAC CCCTGGTCTG
CCCATGGATC TGCAAAATGG GAAATTTTTG GATAATGGTG GTTCTGGATA TATTTTGAAA
CCACATTTCT TAAGAGAGAG TAAATCATAC TTTAACCCAA GTAACATAAA AGAGGGTATG
CCAATTACAC TTACAATAAG GCTCATCAGT GGTATCCAGT TGCCTCTTAC TCATTCATCA
TCTAACAAAG GTGATTCATT AGTAATTATA GAAGTTTTTG GTGTTCCAAA TGATCAAATG
AAGCAGCAGA CTCGTGTAAT TAAAAAAAAT GCTTTTAGTC CAAGATGGAA TGAAACATTC
ACATTTATTA TTCATGTCCC AGAATTGGCA TTGATACGTT TTGTTGTTGA AGGTCAAGGT
TTAATAGCAG GAAATGAATT TCTTGGGCAA TATACTTTGC CACTTCTATG CATGAACAAA
GGTTATCGTC GTATTCCTCT GTTTTCCAGA ATGGGTGAGA GCCTTGAGCC TGCTTCACTG
TTTGTTTATG TTTGTTTCAC AGAGCATCAA CCCGGACACA GCCACTCTGT ACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84304
Clone ID Related Accession (Same CDS sequence) XM_017020178.1
Accession Version XM_017020178.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1830bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGGCACTA
AAATTCAAAA TATTAGTTAA AAATAAGAAA ATAGGAACCT TAAAGGAAAC CCATGAAAGA
AAAGGTTCTG ATAAGCGTGG TAAGGTGGAG GAATGGGAAG AAGAAGTGGC AGATGGAGAG
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGATAAATT CAAAGAATCA
GAAGTATTGG AATCTGTTTT AGGAGACAAT CAAGACAAGG AAACAGGGGT AAAAAAGTTA
CCTGGAGTAA TGCTTTTCAA GAAAAAGAAG ACCAGGAAGC TAAAAATTGC TCTGGCCTTA
TCTGATCTTG TCATTTATAC GAAAGCTGAG AAATTCAAAA GCTTTCAACA TTCAAGATTA
TATCAGCAAT TTAATGAAAA TAATTCTATT GGGGAGACAC AAGCCCGAAA ACTTTCAAAA
TTGCGAGTCC ATGAGTTTAT TTTTCACACC AGGAAGTTCA TTACCAGAAT ATATCCCAAA
GCAACAAGAG CAGACTCTTC TAATTTTAAT CCCCAAGAAT TTTGGAATAT AGGTTGTCAA
ATGGTGGCTT TAAATTTCCA GACCCCTGGT CTGCCCATGG ATCTGCAAAA TGGGAAATTT
TTGGATAATG GTGGTTCTGG ATATATTTTG AAACCACATT TCTTAAGAGA GAGTAAATCA
TACTTTAACC CAAGTAACAT AAAAGAGGGT ATGCCAATTA CACTTACAAT AAGGCTCATC
AGTGGTATCC AGTTGCCTCT TACTCATTCA TCATCTAACA AAGGTGATTC ATTAGTAATT
ATAGAAGTTT TTGGTGTTCC AAATGATCAA ATGAAGCAGC AGACTCGTGT AATTAAAAAA
AATGCTTTTA GTCCAAGATG GAATGAAACA TTCACATTTA TTATTCATGT CCCAGAATTG
GCATTGATAC GTTTTGTTGT TGAAGGTCAA GGTTTAATAG CAGGAAATGA ATTTCTTGGG
CAATATACTT TGCCACTTCT ATGCATGAAC AAAGGTTATC GTCGTATTCC TCTGTTTTCC
AGAATGGGTG AGAGCCTTGA GCCTGCTTCA CTGTTTGTTT ATGTTTGTTT CACAGAGCAT
CAACCCGGAC ACAGCCACTC TGTACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875667.1
CDS260..2089
Translation

Target ORF information:

RefSeq Version XM_017020178.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020178.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGGCACTA
AAATTCAAAA TATTAGTTAA AAATAAGAAA ATAGGAACCT TAAAGGAAAC CCATGAAAGA
AAAGGTTCTG ATAAGCGTGG TAAGGTGGAG GAATGGGAAG AAGAAGTGGC AGATGGAGAG
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGATAAATT CAAAGAATCA
GAAGTATTGG AATCTGTTTT AGGAGACAAT CAAGACAAGG AAACAGGGGT AAAAAAGTTA
CCTGGAGTAA TGCTTTTCAA GAAAAAGAAG ACCAGGAAGC TAAAAATTGC TCTGGCCTTA
TCTGATCTTG TCATTTATAC GAAAGCTGAG AAATTCAAAA GCTTTCAACA TTCAAGATTA
TATCAGCAAT TTAATGAAAA TAATTCTATT GGGGAGACAC AAGCCCGAAA ACTTTCAAAA
TTGCGAGTCC ATGAGTTTAT TTTTCACACC AGGAAGTTCA TTACCAGAAT ATATCCCAAA
GCAACAAGAG CAGACTCTTC TAATTTTAAT CCCCAAGAAT TTTGGAATAT AGGTTGTCAA
ATGGTGGCTT TAAATTTCCA GACCCCTGGT CTGCCCATGG ATCTGCAAAA TGGGAAATTT
TTGGATAATG GTGGTTCTGG ATATATTTTG AAACCACATT TCTTAAGAGA GAGTAAATCA
TACTTTAACC CAAGTAACAT AAAAGAGGGT ATGCCAATTA CACTTACAAT AAGGCTCATC
AGTGGTATCC AGTTGCCTCT TACTCATTCA TCATCTAACA AAGGTGATTC ATTAGTAATT
ATAGAAGTTT TTGGTGTTCC AAATGATCAA ATGAAGCAGC AGACTCGTGT AATTAAAAAA
AATGCTTTTA GTCCAAGATG GAATGAAACA TTCACATTTA TTATTCATGT CCCAGAATTG
GCATTGATAC GTTTTGTTGT TGAAGGTCAA GGTTTAATAG CAGGAAATGA ATTTCTTGGG
CAATATACTT TGCCACTTCT ATGCATGAAC AAAGGTTATC GTCGTATTCC TCTGTTTTCC
AGAATGGGTG AGAGCCTTGA GCCTGCTTCA CTGTTTGTTT ATGTTTGTTT CACAGAGCAT
CAACCCGGAC ACAGCCACTC TGTACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84305
Clone ID Related Accession (Same CDS sequence) XM_017020180.1 , XM_017020181.1 , XM_017020179.1
Accession Version XM_017020180.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGAGTAAAG CTATTGCTTT TGAGATCATT CAGAAATACG AGCCTATCGA AGAAGTTAGG 
AAAGCACACC AAATGTCATT AGAAGGTTTT ACAAGATACA TGGATTCACG TGAATGTCTA
CTGTTTAAAA ATGAATGTAG AAAAGTTTAT CAAGATATGA CTCATCCATT AAATGATTAT
TTTATTTCAT CTTCACATAA CACATATTTG GTATCTGATC AATTATTGGG ACCAAGTGAC
CTTTGGGGAT ATGTAAGTGC CCTTGTGAAA GGATGCCGTT GTTTGGAGAT TGACTGCTGG
GATGGAGCAC AAAATGAACC TGTTGTATAT CATGGCTACA CACTCACAAG CAAACTTCTG
TTTAAAACTG TTATCCAAGC TATACACAAG TATGCATTCA TGACATCTGA CTACCCAGTG
GTGCTCTCTT TAGAAAATCA CTGCTCCACT GCCCAACAAG AAGTAATGGC AGACAATTTG
CAGGCTACTT TTGGAGAGTC CTTGCTTTCT GATATGCTTG ATGATTTTCC TGATACTCTA
CCATCACCAG AGGCACTAAA ATTCAAAATA TTAGTTAAAA ATAAGAAAAT AGGAACCTTA
AAGGAAACCC ATGAAAGAAA AGGTTCTGAT AAGCGTGGTA AGGTGGAGGA ATGGGAAGAA
GAAGTGGCAG ATGGAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GATAAATTCA AAGAATCAGA AGTATTGGAA TCTGTTTTAG GAGACAATCA AGACAAGGAA
ACAGGGGTAA AAAAGTTACC TGGAGTAATG CTTTTCAAGA AAAAGAAGAC CAGGAAGCTA
AAAATTGCTC TGGCCTTATC TGATCTTGTC ATTTATACGA AAGCTGAGAA ATTCAAAAGC
TTTCAACATT CAAGATTATA TCAGCAATTT AATGAAAATA ATTCTATTGG GGAGACACAA
GCCCGAAAAC TTTCAAAATT GCGAGTCCAT GAGTTTATTT TTCACACCAG GAAGTTCATT
ACCAGAATAT ATCCCAAAGC AACAAGAGCA GACTCTTCTA ATTTTAATCC CCAAGAATTT
TGGAATATAG GTTGTCAAAT GGTGGCTTTA AATTTCCAGA CCCCTGGTCT GCCCATGGAT
CTGCAAAATG GGAAATTTTT GGATAATGGT GGTTCTGGAT ATATTTTGAA ACCACATTTC
TTAAGAGAGA GTAAATCATA CTTTAACCCA AGTAACATAA AAGAGGGTAT GCCAATTACA
CTTACAATAA GGCTCATCAG TGGTATCCAG TTGCCTCTTA CTCATTCATC ATCTAACAAA
GGTGATTCAT TAGTAATTAT AGAAGTTTTT GGTGTTCCAA ATGATCAAAT GAAGCAGCAG
ACTCGTGTAA TTAAAAAAAA TGCTTTTAGT CCAAGATGGA ATGAAACATT CACATTTATT
ATTCATGTCC CAGAATTGGC ATTGATACGT TTTGTTGTTG AAGGTCAAGG TTTAATAGCA
GGAAATGAAT TTCTTGGGCA ATATACTTTG CCACTTCTAT GCATGAACAA AGGTTATCGT
CGTATTCCTC TGTTTTCCAG AATGGGTGAG AGCCTTGAGC CTGCTTCACT GTTTGTTTAT
GTTTGTTTCA CAGAGCATCA ACCCGGACAC AGCCACTCTG TACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875669.1
CDS461..2128
Translation

Target ORF information:

RefSeq Version XM_017020180.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020180.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGAGTAAAG CTATTGCTTT TGAGATCATT CAGAAATACG AGCCTATCGA AGAAGTTAGG 
AAAGCACACC AAATGTCATT AGAAGGTTTT ACAAGATACA TGGATTCACG TGAATGTCTA
CTGTTTAAAA ATGAATGTAG AAAAGTTTAT CAAGATATGA CTCATCCATT AAATGATTAT
TTTATTTCAT CTTCACATAA CACATATTTG GTATCTGATC AATTATTGGG ACCAAGTGAC
CTTTGGGGAT ATGTAAGTGC CCTTGTGAAA GGATGCCGTT GTTTGGAGAT TGACTGCTGG
GATGGAGCAC AAAATGAACC TGTTGTATAT CATGGCTACA CACTCACAAG CAAACTTCTG
TTTAAAACTG TTATCCAAGC TATACACAAG TATGCATTCA TGACATCTGA CTACCCAGTG
GTGCTCTCTT TAGAAAATCA CTGCTCCACT GCCCAACAAG AAGTAATGGC AGACAATTTG
CAGGCTACTT TTGGAGAGTC CTTGCTTTCT GATATGCTTG ATGATTTTCC TGATACTCTA
CCATCACCAG AGGCACTAAA ATTCAAAATA TTAGTTAAAA ATAAGAAAAT AGGAACCTTA
AAGGAAACCC ATGAAAGAAA AGGTTCTGAT AAGCGTGGTA AGGTGGAGGA ATGGGAAGAA
GAAGTGGCAG ATGGAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GATAAATTCA AAGAATCAGA AGTATTGGAA TCTGTTTTAG GAGACAATCA AGACAAGGAA
ACAGGGGTAA AAAAGTTACC TGGAGTAATG CTTTTCAAGA AAAAGAAGAC CAGGAAGCTA
AAAATTGCTC TGGCCTTATC TGATCTTGTC ATTTATACGA AAGCTGAGAA ATTCAAAAGC
TTTCAACATT CAAGATTATA TCAGCAATTT AATGAAAATA ATTCTATTGG GGAGACACAA
GCCCGAAAAC TTTCAAAATT GCGAGTCCAT GAGTTTATTT TTCACACCAG GAAGTTCATT
ACCAGAATAT ATCCCAAAGC AACAAGAGCA GACTCTTCTA ATTTTAATCC CCAAGAATTT
TGGAATATAG GTTGTCAAAT GGTGGCTTTA AATTTCCAGA CCCCTGGTCT GCCCATGGAT
CTGCAAAATG GGAAATTTTT GGATAATGGT GGTTCTGGAT ATATTTTGAA ACCACATTTC
TTAAGAGAGA GTAAATCATA CTTTAACCCA AGTAACATAA AAGAGGGTAT GCCAATTACA
CTTACAATAA GGCTCATCAG TGGTATCCAG TTGCCTCTTA CTCATTCATC ATCTAACAAA
GGTGATTCAT TAGTAATTAT AGAAGTTTTT GGTGTTCCAA ATGATCAAAT GAAGCAGCAG
ACTCGTGTAA TTAAAAAAAA TGCTTTTAGT CCAAGATGGA ATGAAACATT CACATTTATT
ATTCATGTCC CAGAATTGGC ATTGATACGT TTTGTTGTTG AAGGTCAAGG TTTAATAGCA
GGAAATGAAT TTCTTGGGCA ATATACTTTG CCACTTCTAT GCATGAACAA AGGTTATCGT
CGTATTCCTC TGTTTTCCAG AATGGGTGAG AGCCTTGAGC CTGCTTCACT GTTTGTTTAT
GTTTGTTTCA CAGAGCATCA ACCCGGACAC AGCCACTCTG TACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84305
Clone ID Related Accession (Same CDS sequence) XM_017020180.1 , XM_017020181.1 , XM_017020179.1
Accession Version XM_017020181.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGAGTAAAG CTATTGCTTT TGAGATCATT CAGAAATACG AGCCTATCGA AGAAGTTAGG 
AAAGCACACC AAATGTCATT AGAAGGTTTT ACAAGATACA TGGATTCACG TGAATGTCTA
CTGTTTAAAA ATGAATGTAG AAAAGTTTAT CAAGATATGA CTCATCCATT AAATGATTAT
TTTATTTCAT CTTCACATAA CACATATTTG GTATCTGATC AATTATTGGG ACCAAGTGAC
CTTTGGGGAT ATGTAAGTGC CCTTGTGAAA GGATGCCGTT GTTTGGAGAT TGACTGCTGG
GATGGAGCAC AAAATGAACC TGTTGTATAT CATGGCTACA CACTCACAAG CAAACTTCTG
TTTAAAACTG TTATCCAAGC TATACACAAG TATGCATTCA TGACATCTGA CTACCCAGTG
GTGCTCTCTT TAGAAAATCA CTGCTCCACT GCCCAACAAG AAGTAATGGC AGACAATTTG
CAGGCTACTT TTGGAGAGTC CTTGCTTTCT GATATGCTTG ATGATTTTCC TGATACTCTA
CCATCACCAG AGGCACTAAA ATTCAAAATA TTAGTTAAAA ATAAGAAAAT AGGAACCTTA
AAGGAAACCC ATGAAAGAAA AGGTTCTGAT AAGCGTGGTA AGGTGGAGGA ATGGGAAGAA
GAAGTGGCAG ATGGAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GATAAATTCA AAGAATCAGA AGTATTGGAA TCTGTTTTAG GAGACAATCA AGACAAGGAA
ACAGGGGTAA AAAAGTTACC TGGAGTAATG CTTTTCAAGA AAAAGAAGAC CAGGAAGCTA
AAAATTGCTC TGGCCTTATC TGATCTTGTC ATTTATACGA AAGCTGAGAA ATTCAAAAGC
TTTCAACATT CAAGATTATA TCAGCAATTT AATGAAAATA ATTCTATTGG GGAGACACAA
GCCCGAAAAC TTTCAAAATT GCGAGTCCAT GAGTTTATTT TTCACACCAG GAAGTTCATT
ACCAGAATAT ATCCCAAAGC AACAAGAGCA GACTCTTCTA ATTTTAATCC CCAAGAATTT
TGGAATATAG GTTGTCAAAT GGTGGCTTTA AATTTCCAGA CCCCTGGTCT GCCCATGGAT
CTGCAAAATG GGAAATTTTT GGATAATGGT GGTTCTGGAT ATATTTTGAA ACCACATTTC
TTAAGAGAGA GTAAATCATA CTTTAACCCA AGTAACATAA AAGAGGGTAT GCCAATTACA
CTTACAATAA GGCTCATCAG TGGTATCCAG TTGCCTCTTA CTCATTCATC ATCTAACAAA
GGTGATTCAT TAGTAATTAT AGAAGTTTTT GGTGTTCCAA ATGATCAAAT GAAGCAGCAG
ACTCGTGTAA TTAAAAAAAA TGCTTTTAGT CCAAGATGGA ATGAAACATT CACATTTATT
ATTCATGTCC CAGAATTGGC ATTGATACGT TTTGTTGTTG AAGGTCAAGG TTTAATAGCA
GGAAATGAAT TTCTTGGGCA ATATACTTTG CCACTTCTAT GCATGAACAA AGGTTATCGT
CGTATTCCTC TGTTTTCCAG AATGGGTGAG AGCCTTGAGC CTGCTTCACT GTTTGTTTAT
GTTTGTTTCA CAGAGCATCA ACCCGGACAC AGCCACTCTG TACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875670.1
CDS526..2193
Translation

Target ORF information:

RefSeq Version XM_017020181.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020181.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGAGTAAAG CTATTGCTTT TGAGATCATT CAGAAATACG AGCCTATCGA AGAAGTTAGG 
AAAGCACACC AAATGTCATT AGAAGGTTTT ACAAGATACA TGGATTCACG TGAATGTCTA
CTGTTTAAAA ATGAATGTAG AAAAGTTTAT CAAGATATGA CTCATCCATT AAATGATTAT
TTTATTTCAT CTTCACATAA CACATATTTG GTATCTGATC AATTATTGGG ACCAAGTGAC
CTTTGGGGAT ATGTAAGTGC CCTTGTGAAA GGATGCCGTT GTTTGGAGAT TGACTGCTGG
GATGGAGCAC AAAATGAACC TGTTGTATAT CATGGCTACA CACTCACAAG CAAACTTCTG
TTTAAAACTG TTATCCAAGC TATACACAAG TATGCATTCA TGACATCTGA CTACCCAGTG
GTGCTCTCTT TAGAAAATCA CTGCTCCACT GCCCAACAAG AAGTAATGGC AGACAATTTG
CAGGCTACTT TTGGAGAGTC CTTGCTTTCT GATATGCTTG ATGATTTTCC TGATACTCTA
CCATCACCAG AGGCACTAAA ATTCAAAATA TTAGTTAAAA ATAAGAAAAT AGGAACCTTA
AAGGAAACCC ATGAAAGAAA AGGTTCTGAT AAGCGTGGTA AGGTGGAGGA ATGGGAAGAA
GAAGTGGCAG ATGGAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GATAAATTCA AAGAATCAGA AGTATTGGAA TCTGTTTTAG GAGACAATCA AGACAAGGAA
ACAGGGGTAA AAAAGTTACC TGGAGTAATG CTTTTCAAGA AAAAGAAGAC CAGGAAGCTA
AAAATTGCTC TGGCCTTATC TGATCTTGTC ATTTATACGA AAGCTGAGAA ATTCAAAAGC
TTTCAACATT CAAGATTATA TCAGCAATTT AATGAAAATA ATTCTATTGG GGAGACACAA
GCCCGAAAAC TTTCAAAATT GCGAGTCCAT GAGTTTATTT TTCACACCAG GAAGTTCATT
ACCAGAATAT ATCCCAAAGC AACAAGAGCA GACTCTTCTA ATTTTAATCC CCAAGAATTT
TGGAATATAG GTTGTCAAAT GGTGGCTTTA AATTTCCAGA CCCCTGGTCT GCCCATGGAT
CTGCAAAATG GGAAATTTTT GGATAATGGT GGTTCTGGAT ATATTTTGAA ACCACATTTC
TTAAGAGAGA GTAAATCATA CTTTAACCCA AGTAACATAA AAGAGGGTAT GCCAATTACA
CTTACAATAA GGCTCATCAG TGGTATCCAG TTGCCTCTTA CTCATTCATC ATCTAACAAA
GGTGATTCAT TAGTAATTAT AGAAGTTTTT GGTGTTCCAA ATGATCAAAT GAAGCAGCAG
ACTCGTGTAA TTAAAAAAAA TGCTTTTAGT CCAAGATGGA ATGAAACATT CACATTTATT
ATTCATGTCC CAGAATTGGC ATTGATACGT TTTGTTGTTG AAGGTCAAGG TTTAATAGCA
GGAAATGAAT TTCTTGGGCA ATATACTTTG CCACTTCTAT GCATGAACAA AGGTTATCGT
CGTATTCCTC TGTTTTCCAG AATGGGTGAG AGCCTTGAGC CTGCTTCACT GTTTGTTTAT
GTTTGTTTCA CAGAGCATCA ACCCGGACAC AGCCACTCTG TACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84305
Clone ID Related Accession (Same CDS sequence) XM_017020180.1 , XM_017020181.1 , XM_017020179.1
Accession Version XM_017020179.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGAGTAAAG CTATTGCTTT TGAGATCATT CAGAAATACG AGCCTATCGA AGAAGTTAGG 
AAAGCACACC AAATGTCATT AGAAGGTTTT ACAAGATACA TGGATTCACG TGAATGTCTA
CTGTTTAAAA ATGAATGTAG AAAAGTTTAT CAAGATATGA CTCATCCATT AAATGATTAT
TTTATTTCAT CTTCACATAA CACATATTTG GTATCTGATC AATTATTGGG ACCAAGTGAC
CTTTGGGGAT ATGTAAGTGC CCTTGTGAAA GGATGCCGTT GTTTGGAGAT TGACTGCTGG
GATGGAGCAC AAAATGAACC TGTTGTATAT CATGGCTACA CACTCACAAG CAAACTTCTG
TTTAAAACTG TTATCCAAGC TATACACAAG TATGCATTCA TGACATCTGA CTACCCAGTG
GTGCTCTCTT TAGAAAATCA CTGCTCCACT GCCCAACAAG AAGTAATGGC AGACAATTTG
CAGGCTACTT TTGGAGAGTC CTTGCTTTCT GATATGCTTG ATGATTTTCC TGATACTCTA
CCATCACCAG AGGCACTAAA ATTCAAAATA TTAGTTAAAA ATAAGAAAAT AGGAACCTTA
AAGGAAACCC ATGAAAGAAA AGGTTCTGAT AAGCGTGGTA AGGTGGAGGA ATGGGAAGAA
GAAGTGGCAG ATGGAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GATAAATTCA AAGAATCAGA AGTATTGGAA TCTGTTTTAG GAGACAATCA AGACAAGGAA
ACAGGGGTAA AAAAGTTACC TGGAGTAATG CTTTTCAAGA AAAAGAAGAC CAGGAAGCTA
AAAATTGCTC TGGCCTTATC TGATCTTGTC ATTTATACGA AAGCTGAGAA ATTCAAAAGC
TTTCAACATT CAAGATTATA TCAGCAATTT AATGAAAATA ATTCTATTGG GGAGACACAA
GCCCGAAAAC TTTCAAAATT GCGAGTCCAT GAGTTTATTT TTCACACCAG GAAGTTCATT
ACCAGAATAT ATCCCAAAGC AACAAGAGCA GACTCTTCTA ATTTTAATCC CCAAGAATTT
TGGAATATAG GTTGTCAAAT GGTGGCTTTA AATTTCCAGA CCCCTGGTCT GCCCATGGAT
CTGCAAAATG GGAAATTTTT GGATAATGGT GGTTCTGGAT ATATTTTGAA ACCACATTTC
TTAAGAGAGA GTAAATCATA CTTTAACCCA AGTAACATAA AAGAGGGTAT GCCAATTACA
CTTACAATAA GGCTCATCAG TGGTATCCAG TTGCCTCTTA CTCATTCATC ATCTAACAAA
GGTGATTCAT TAGTAATTAT AGAAGTTTTT GGTGTTCCAA ATGATCAAAT GAAGCAGCAG
ACTCGTGTAA TTAAAAAAAA TGCTTTTAGT CCAAGATGGA ATGAAACATT CACATTTATT
ATTCATGTCC CAGAATTGGC ATTGATACGT TTTGTTGTTG AAGGTCAAGG TTTAATAGCA
GGAAATGAAT TTCTTGGGCA ATATACTTTG CCACTTCTAT GCATGAACAA AGGTTATCGT
CGTATTCCTC TGTTTTCCAG AATGGGTGAG AGCCTTGAGC CTGCTTCACT GTTTGTTTAT
GTTTGTTTCA CAGAGCATCA ACCCGGACAC AGCCACTCTG TACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875668.1
CDS567..2234
Translation

Target ORF information:

RefSeq Version XM_017020179.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020179.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGAGTAAAG CTATTGCTTT TGAGATCATT CAGAAATACG AGCCTATCGA AGAAGTTAGG 
AAAGCACACC AAATGTCATT AGAAGGTTTT ACAAGATACA TGGATTCACG TGAATGTCTA
CTGTTTAAAA ATGAATGTAG AAAAGTTTAT CAAGATATGA CTCATCCATT AAATGATTAT
TTTATTTCAT CTTCACATAA CACATATTTG GTATCTGATC AATTATTGGG ACCAAGTGAC
CTTTGGGGAT ATGTAAGTGC CCTTGTGAAA GGATGCCGTT GTTTGGAGAT TGACTGCTGG
GATGGAGCAC AAAATGAACC TGTTGTATAT CATGGCTACA CACTCACAAG CAAACTTCTG
TTTAAAACTG TTATCCAAGC TATACACAAG TATGCATTCA TGACATCTGA CTACCCAGTG
GTGCTCTCTT TAGAAAATCA CTGCTCCACT GCCCAACAAG AAGTAATGGC AGACAATTTG
CAGGCTACTT TTGGAGAGTC CTTGCTTTCT GATATGCTTG ATGATTTTCC TGATACTCTA
CCATCACCAG AGGCACTAAA ATTCAAAATA TTAGTTAAAA ATAAGAAAAT AGGAACCTTA
AAGGAAACCC ATGAAAGAAA AGGTTCTGAT AAGCGTGGTA AGGTGGAGGA ATGGGAAGAA
GAAGTGGCAG ATGGAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GATAAATTCA AAGAATCAGA AGTATTGGAA TCTGTTTTAG GAGACAATCA AGACAAGGAA
ACAGGGGTAA AAAAGTTACC TGGAGTAATG CTTTTCAAGA AAAAGAAGAC CAGGAAGCTA
AAAATTGCTC TGGCCTTATC TGATCTTGTC ATTTATACGA AAGCTGAGAA ATTCAAAAGC
TTTCAACATT CAAGATTATA TCAGCAATTT AATGAAAATA ATTCTATTGG GGAGACACAA
GCCCGAAAAC TTTCAAAATT GCGAGTCCAT GAGTTTATTT TTCACACCAG GAAGTTCATT
ACCAGAATAT ATCCCAAAGC AACAAGAGCA GACTCTTCTA ATTTTAATCC CCAAGAATTT
TGGAATATAG GTTGTCAAAT GGTGGCTTTA AATTTCCAGA CCCCTGGTCT GCCCATGGAT
CTGCAAAATG GGAAATTTTT GGATAATGGT GGTTCTGGAT ATATTTTGAA ACCACATTTC
TTAAGAGAGA GTAAATCATA CTTTAACCCA AGTAACATAA AAGAGGGTAT GCCAATTACA
CTTACAATAA GGCTCATCAG TGGTATCCAG TTGCCTCTTA CTCATTCATC ATCTAACAAA
GGTGATTCAT TAGTAATTAT AGAAGTTTTT GGTGTTCCAA ATGATCAAAT GAAGCAGCAG
ACTCGTGTAA TTAAAAAAAA TGCTTTTAGT CCAAGATGGA ATGAAACATT CACATTTATT
ATTCATGTCC CAGAATTGGC ATTGATACGT TTTGTTGTTG AAGGTCAAGG TTTAATAGCA
GGAAATGAAT TTCTTGGGCA ATATACTTTG CCACTTCTAT GCATGAACAA AGGTTATCGT
CGTATTCCTC TGTTTTCCAG AATGGGTGAG AGCCTTGAGC CTGCTTCACT GTTTGTTTAT
GTTTGTTTCA CAGAGCATCA ACCCGGACAC AGCCACTCTG TACCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46213
Clone ID Related Accession (Same CDS sequence) XM_005253519.2
Accession Version XM_005253519.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1950bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005253519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGACATCT
GACTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCA CTGCCCAACA AGAAGTAATG
GCAGACAATT TGCAGGCTAC TTTTGGAGAG TCCTTGCTTT CTGATATGCT TGATGATTTT
CCTGATACTC TACCATCACC AGAGGCACTA AAATTCAAAA TATTAGTTAA AAATAAGAAA
ATAGGAACCT TAAAGGAAAC CCATGAAAGA AAAGGTTCTG ATAAGCGTGG TAAGGTGGAG
GAATGGGAAG AAGAAGTGGC AGATGGAGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GAGGAGGAGG AGGATAAATT CAAAGAATCA GAAGTATTGG AATCTGTTTT AGGAGACAAT
CAAGACAAGG AAACAGGGGT AAAAAAGTTA CCTGGAGTAA TGCTTTTCAA GAAAAAGAAG
ACCAGGAAGC TAAAAATTGC TCTGGCCTTA TCTGATCTTG TCATTTATAC GAAAGCTGAG
AAATTCAAAA GCTTTCAACA TTCAAGATTA TATCAGCAAT TTAATGAAAA TAATTCTATT
GGGGAGACAC AAGCCCGAAA ACTTTCAAAA TTGCGAGTCC ATGAGTTTAT TTTTCACACC
AGGAAGTTCA TTACCAGAAT ATATCCCAAA GCAACAAGAG CAGACTCTTC TAATTTTAAT
CCCCAAGAAT TTTGGAATAT AGGTTGTCAA ATGGTGGCTT TAAATTTCCA GACCCCTGGT
CTGCCCATGG ATCTGCAAAA TGGGAAATTT TTGGATAATG GTGGTTCTGG ATATATTTTG
AAACCACATT TCTTAAGAGA GAGTAAATCA TACTTTAACC CAAGTAACAT AAAAGAGGGT
ATGCCAATTA CACTTACAAT AAGGCTCATC AGTGGTATCC AGTTGCCTCT TACTCATTCA
TCATCTAACA AAGGTGATTC ATTAGTAATT ATAGAAGTTT TTGGTGTTCC AAATGATCAA
ATGAAGCAGC AGACTCGTGT AATTAAAAAA AATGCTTTTA GTCCAAGATG GAATGAAACA
TTCACATTTA TTATTCATGT CCCAGAATTG GCATTGATAC GTTTTGTTGT TGAAGGTCAA
GGTTTAATAG CAGGAAATGA ATTTCTTGGG CAATATACTT TGCCACTTCT ATGCATGAAC
AAAGGTTATC GTCGTATTCC TCTGTTTTCC AGAATGGGTG AGAGCCTTGA GCCTGCTTCA
CTGTTTGTTT ATGTTTGGTA CGTCAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005253576.1
CDS260..2209
Misc Feature(1)470..2203
Misc Feature(2)473..721
Misc Feature(3)719..1738
Misc Feature(4)order(767..772,857..859,863..865,902..904,1004..1006,1148..1150,1154..1156,1514..1516,1595..1597,1601..1603)
Misc Feature(5)order(767..769,902..904)
Misc Feature(6)order(770..772,857..859,863..865,1004..1006)
Misc Feature(7)1823..2200
Misc Feature(8)order(1895..1897,1967..1969,2054..2056,2060..2062,2078..2080,2084..2086)
Translation

Target ORF information:

RefSeq Version XM_005253519.2
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C zeta 1 (PLCZ1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005253519.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAAATGA GATGGTTTTT GTCAAAGATT CAGGATGACT TCAGAGGTGG AAAAATTAAC 
CTAGAAAAAA CTCAGAGGTT ACTTGAAAAA TTAGATATTC GGTGCAGTTA TATTCATGTG
AAACAGATTT TTAAGGACAA TGACAGGCTG AAACAAGGAA GAATCACCAT AGAAGAATTT
AGAGCAATTT ATCGAATTAT CACGCACAGA GAAGAAATTA TTGAGATTTT CAACACATAT
TCTGAAAACC GGAAAATTCT TTTAGCAAGT AATCTGGCTC AATTTCTGAC ACAAGAACAA
TATGCAGCTG AGATGAGTAA AGCTATTGCT TTTGAGATCA TTCAGAAATA CGAGCCTATC
GAAGAAGTTA GGAAAGCACA CCAAATGTCA TTAGAAGGTT TTACAAGATA CATGGATTCA
CGTGAATGTC TACTGTTTAA AAATGAATGT AGAAAAGTTT ATCAAGATAT GACTCATCCA
TTAAATGATT ATTTTATTTC ATCTTCACAT AACACATATT TGGTATCTGA TCAATTATTG
GGACCAAGTG ACCTTTGGGG ATATGTAAGT GCCCTTGTGA AAGGATGCCG TTGTTTGGAG
ATTGACTGCT GGGATGGAGC ACAAAATGAA CCTGTTGTAT ATCATGGCTA CACACTCACA
AGCAAACTTC TGTTTAAAAC TGTTATCCAA GCTATACACA AGTATGCATT CATGACATCT
GACTACCCAG TGGTGCTCTC TTTAGAAAAT CACTGCTCCA CTGCCCAACA AGAAGTAATG
GCAGACAATT TGCAGGCTAC TTTTGGAGAG TCCTTGCTTT CTGATATGCT TGATGATTTT
CCTGATACTC TACCATCACC AGAGGCACTA AAATTCAAAA TATTAGTTAA AAATAAGAAA
ATAGGAACCT TAAAGGAAAC CCATGAAAGA AAAGGTTCTG ATAAGCGTGG TAAGGTGGAG
GAATGGGAAG AAGAAGTGGC AGATGGAGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
GAGGAGGAGG AGGATAAATT CAAAGAATCA GAAGTATTGG AATCTGTTTT AGGAGACAAT
CAAGACAAGG AAACAGGGGT AAAAAAGTTA CCTGGAGTAA TGCTTTTCAA GAAAAAGAAG
ACCAGGAAGC TAAAAATTGC TCTGGCCTTA TCTGATCTTG TCATTTATAC GAAAGCTGAG
AAATTCAAAA GCTTTCAACA TTCAAGATTA TATCAGCAAT TTAATGAAAA TAATTCTATT
GGGGAGACAC AAGCCCGAAA ACTTTCAAAA TTGCGAGTCC ATGAGTTTAT TTTTCACACC
AGGAAGTTCA TTACCAGAAT ATATCCCAAA GCAACAAGAG CAGACTCTTC TAATTTTAAT
CCCCAAGAAT TTTGGAATAT AGGTTGTCAA ATGGTGGCTT TAAATTTCCA GACCCCTGGT
CTGCCCATGG ATCTGCAAAA TGGGAAATTT TTGGATAATG GTGGTTCTGG ATATATTTTG
AAACCACATT TCTTAAGAGA GAGTAAATCA TACTTTAACC CAAGTAACAT AAAAGAGGGT
ATGCCAATTA CACTTACAAT AAGGCTCATC AGTGGTATCC AGTTGCCTCT TACTCATTCA
TCATCTAACA AAGGTGATTC ATTAGTAATT ATAGAAGTTT TTGGTGTTCC AAATGATCAA
ATGAAGCAGC AGACTCGTGT AATTAAAAAA AATGCTTTTA GTCCAAGATG GAATGAAACA
TTCACATTTA TTATTCATGT CCCAGAATTG GCATTGATAC GTTTTGTTGT TGAAGGTCAA
GGTTTAATAG CAGGAAATGA ATTTCTTGGG CAATATACTT TGCCACTTCT ATGCATGAAC
AAAGGTTATC GTCGTATTCC TCTGTTTTCC AGAATGGGTG AGAGCCTTGA GCCTGCTTCA
CTGTTTGTTT ATGTTTGGTA CGTCAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

An association between sperm PLCζ levels and varicocele?
Journal of assisted reproduction and genetics33(12)1649-1655(2016 Dec)
Janghorban-Laricheh E,Ghazavi-Khorasgani N,Tavalaee M,Zohrabi D,Abbasi H,Nasr-Esfahani MH


book

Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.
Human molecular genetics25(5)878-91(2016 Mar)
Escoffier J,Lee HC,Yassine S,Zouari R,Martinez G,Karaouzène T,Coutton C,Kherraf ZE,Halouani L,Triki C,Nef S,Thierry-Mieg N,Savinov SN,Fissore R,Ray PF,Arnoult C


book

Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report.
Journal of assisted reproduction and genetics32(9)1415-9(2015 Sep)
Chithiwala ZH,Lee HC,Hill DL,Jellerette-Nolan T,Fissore R,Grow D,Dumesic DA


book

Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.
Molecular human reproduction21(9)702-10(2015 Sep)
Nomikos M,Sanders JR,Kashir J,Sanusi R,Buntwal L,Love D,Ashley P,Sanders D,Knaggs P,Bunkheila A,Swann K,Lai FA


book

NYD-SP27, a novel intrinsic decapacitation factor in sperm.
Asian journal of andrology11(2)229-39(2009 Mar)
Bi Y,Xu WM,Wong HY,Zhu H,Zhou ZM,Chan HC,Sha JH


 
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