DOT1L gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010798345.2	XP_010796647.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
XM_015464178.1	XP_015319664.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
XM_010807071.2	XP_010805373.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
XM_010807066.3	XP_010805368.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_010807073.3	XP_010805375.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X9
XM_010807068.2	XP_010805370.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
XM_015464176.1	XP_015319662.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_015464180.1	XP_015319666.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
XM_024995042.1	XP_024850810.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
XM_005209068.3	XP_005209125.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_003586273.4	XP_003586321.2	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
XM_010807072.2	XP_010805374.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
XM_010807073.2	XP_010805375.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
XM_015464179.1	XP_015319665.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
XM_015464182.1	XP_015319668.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
XM_010807068.3	XP_010805370.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
XM_010807066.2	XP_010805368.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_015464181.1	XP_015319667.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6
XM_024995041.1	XP_024850809.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
XM_010807071.3	XP_010805373.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
XM_003586273.5	XP_003586321.2	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
XM_015464177.1	XP_015319663.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_015464183.1	XP_015319669.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
XM_010807067.2	XP_010805369.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
XM_015472118.1	XP_015327604.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6
XM_005209068.4	XP_005209125.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_010807067.3	XP_010805369.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
XM_010807072.3	XP_010805374.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236579	XM_010807073.3 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa236571	XM_005209068.4 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236571	XM_010807066.3 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236572	XM_010807067.3 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236573	XM_024995041.1 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236574	XM_010807068.3 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236575	XM_010807071.3 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236576	XM_010807072.3 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236577	XM_003586273.5 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236578	XM_024995042.1 Latest version!	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa90289	XM_015472118.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90289	XM_015464181.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa21246	XM_015464182.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa21246	XM_003586273.4 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa47733	XM_015464180.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa47733	XM_010807072.2 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa05346	XM_010798345.2 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa05346	XM_010807067.2 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa21243	XM_005209068.3 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa21243	XM_010807066.2 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa21243	XM_015464176.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa21243	XM_015464177.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa21244	XM_015464179.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa21244	XM_010807071.2 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa21247	XM_015464183.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa21247	XM_010807073.2 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa47730	XM_010807068.2 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa47730	XM_015464178.1 Latest version!	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

DOT1L ( XM_010807073.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805375.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa236579
Clone ID Related Accession (Same CDS sequence)	XM_010807073.3
Accession Version	XM_010807073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG 
TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC 
TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG 
TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC 
ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG 
GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC 
ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT 
GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG 
AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG 
ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG 
CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT 
CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG 
CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA 
CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC 
CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC 
CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG 
AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG 
CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC 
CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG 
GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC 
TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG 
CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG 
CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA 
CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC 
TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG 
CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG 
CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG 
CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA 
CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC 
ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG 
AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC 
AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC 
CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT 
GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT 
GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA 
TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT 
GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG 
GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC 
TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC 
CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA 
GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT 
TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG 
CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC 
TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT 
TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC 
ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC 
GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC 
TCCCCACTGA CTTCGGACGA GGAGCCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT 
GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT 
GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC 
AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC 
CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG 
GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC 
ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG 
GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC 
CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC 
AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG 
GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG 
CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG 
TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA 
GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC 
GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC 
CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG 
ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC 
GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT 
GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG 
CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG 
CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA 
CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG 
CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC 
TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG 
CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG 
GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT 
TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805375.1
CDS	138..4634
Translation	MLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807073.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807073.3

ORF Insert Sequence:

ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG 
TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC 
TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG 
TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC 
ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG 
GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC 
ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT 
GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG 
AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG 
ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG 
CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT 
CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG 
CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA 
CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC 
CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC 
CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG 
AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG 
CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC 
CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG 
GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC 
TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG 
CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG 
CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA 
CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC 
TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG 
CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG 
CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG 
CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA 
CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC 
ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG 
AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC 
AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC 
CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT 
GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT 
GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA 
TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT 
GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG 
GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC 
TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC 
CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA 
GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT 
TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG 
CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC 
TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT 
TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC 
ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC 
GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC 
TCCCCACTGA CTTCGGACGA GGAGCCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT 
GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT 
GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC 
AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC 
CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG 
GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC 
ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG 
GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC 
CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC 
AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG 
GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG 
CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG 
TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA 
GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC 
GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC 
CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG 
ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC 
GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT 
GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG 
CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG 
CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA 
CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG 
CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC 
TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG 
CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG 
GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT 
TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807066.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805368.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X1, mRNA.

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CloneID	OBa236571
Clone ID Related Accession (Same CDS sequence)	XM_010807066.3 , XM_005209068.4
Accession Version	XM_010807066.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805368.1
CDS	76..5220
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807066.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807066.3

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_005209068.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209125.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X2, mRNA.

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CloneID	OBa236571
Clone ID Related Accession (Same CDS sequence)	XM_010807066.3 , XM_005209068.4
Accession Version	XM_005209068.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209068.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209125.1
CDS	76..5220
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_005209068.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209068.4

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807067.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805369.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa236572
Clone ID Related Accession (Same CDS sequence)	XM_010807067.3
Accession Version	XM_010807067.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807067.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805369.1
CDS	76..5217
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807067.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807067.3

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_024995041.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850809.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X4, mRNA.

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CloneID	OBa236573
Clone ID Related Accession (Same CDS sequence)	XM_024995041.1
Accession Version	XM_024995041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCC GTCATGCCCC AGACTCGGCT GACAAACACC ACGATGCTGC TCATGAAATC 
ATCGAGACCA TCAGATGGGT CTGTGAAGAA ATCCCGGATC TCAAGCTTGC TATGGAGAAT 
TACGTTTTAA TTGACTACGA TACCAAAAGC TTTGAGAGTA TGCAGAGGCT CTGTGACAAG 
TACAACCGGG CCATCGACAG CATCCACCAG CTGTGGAAGG GCACCACACA GCCCATGAAG 
CTGAACACAC GGCCATCCAC GGGGCTCCTG CGGCACATCC TGCAGCAGGT CTACAACCAC 
TCAGTGACCG ACCCTGAGAA GCTCAACAAC TACGAGCCCT TCTCCCCAGA GGTGTATGGG 
GAGACCTCCT TCGACCTGGT CGCCCAGATG ATCGATGAGA TCAAGATGAC CGAAGATGAC 
CTGTTCGTGG ACCTGGGCAG TGGAGTGGGC CAGGTTGTGC TGCAGGTTGC CGCGGCCACC 
AACTGCAAAC ATCACTACGG TGTAGAGAAA GCTGACATCC CAGCCAAGTA CGCGGAGACC 
ATGGACCGAG AGTTCAGGAA GTGGATGAAA TGGTATGGAA AAAAGCATGC AGAATACACA 
CTGGAGAGGG GGGACTTCCT CTCGGAGGAG TGGAGAGAAC GGATTGCTAA CACAAGTGTT 
GTATTTGTGA ATAACTTTGC CTTTGGTCCT GAGGTGGATC ACCAGCTGAA GGAGCGGTTT 
GCAAACATGA AGGAAGGTGG CAGAATCGTG TCCTCGAAGC CCTTTGCGCC TCTGAACTTC 
AGAATAAACA GTAGAAACTT GAGTGACATC GGCACCATCA TGCGCGTCGT GGAGCTGTCG 
CCGCTAAAAG GCTCGGTGTC ATGGACGGGG AAGCCAGTCT CCTACTACCT GCACACCATC 
GACCGCACCA TACTTGAAAA CTATTTTTCT AGTCTAAAAA ATCCAAAACT CAGGGAGGAA 
CAAGAGGCAG CTCGGCGCCG GCAGCAGCGG GAGAACAAGA GCAACACAAC CACGCCCACC 
AAGGTGCCCG AGAGCAAGGC AGCTGTGCCG GCGGACACCC CCGTGGACTC TGGTGCTGAG 
GAGGAGAAAG CCGGGACGGC CACTGTCAAG AAGCCATCTC CCTCCAAGGC CCGGAAGAAG 
AAGCTGAACA AGAAGGGCCG GAAGATGGTG GGTCGGAAGC GTGGGCGCCC CAAGAAGATG 
AGTGCCACCA ACCCCGAGCG CAAGCCCAAG AAGACCCAGA GCTCACTGGA CCTGCTGCAC 
GCCCAGACCG CGTCACGAGC GGCCTCCCCC TTGCCGCAGG ACGCGCACAG GCCACCTCAC 
AGCCCATTCT ACCAGCTACC TCCCAGCGTG CAGCGGCCTA CCCCCGAGCA GCTGCTGCTG 
GCACCCACCC CGCCCGCACT GCACAGGCTG CTAGAGTCCT TCAGGATTCA GTACCTACAG 
TTCCTGGCAT ACACGAAGAC CCCTCAGTAC AAGGCCAGCC TGCAGCAGCT CCTGGACCAG 
GAGAAGGAGA AGAATGCCCG GTTGCTAGGC GCAGCGCAGC AGCTATTTGG CCACTGCCAA 
GCTCAGAAGG AGGAGATCAA GAGGCTCTTC CAGCAGAAAC TGGACGAGCT GGGAGTGAAG 
GCGCTGACCT ACAACGACCT GATCCAAGCG CAGAAGGAGA TCTCGGCTCA CAACCAGCAG 
TTGAGGGAGC AGACGGAGCA GCTGGAGAAG GGCAACTGGG AGCTGAGGAG CCAGAGCCTG 
AGGCTGCTCA AGGCACGATG CGAGGAGCTG AAGCTGGACT GGTCCACGCT GTCGCTGGAG 
AATCTGCTGA AGGAGAAGCA GGCCCTGAAG AGCCAGATCT CAGAGAAGCA GAGGCACTGC 
CTGGAGCTGC AGATCAGCAT CGTGGAGCTG GAGAAGAGCC AGCGGCAGCA GGAGTTGCTG 
CAGCTCAAGT CCTGCGTGCC GCCTGATGAG GCACTGGCTC TGCAGCTTCG TGGGAAGCCA 
GAGGCTGAGC CTAGCCGGCT GCACTTGGAG CTGGACTGCG CCCGCTTCTC CCTGCCACCC 
TTCAGCAGCT TGAGCCCCGA GCTCTCTATG AATGGCCACG CAGCCGGCTA TGAGCTCTGC 
AGCGCACTGG GTCGGCCCTC GCCCAAGCAG AACACCCCCC AGTACCTGGC CTCCCCGCTA 
GACCAGGAGG TCGTGCCCTG CACCCCTAGC CACAGCGGCC GGCCACGGCT AGAGAAGCTG 
TCCAGCCTGG CCCTGCCTGA CTACACCAGG CTATCCCCTG CCAAGCTGGT GCTGCGGCGC 
CACCTGAGCC AGGACCACGC GGCCAGTGGC AAGGCAGCTG CCAGTGAGCT GCACCCACGA 
GCCGAGCATG CCAAGGAGAA TGGGCTCCCT TACCAGAGCC CTGGCATCAC TAATGGCATC 
AAGCTGAGCC CACAAGACCC CCGACCTTCA TCCCCCACGG CTTTACAGAT GGGAGAGAAG 
GGCCCCGAGA AGGGTGTGAA GGAGCGTGCC TACGCCAGCA GTGGGGAGGC CATCACCAGC 
CTGCCCGTCA GCATCCCGCT GAGCACCGTG CAGCCCAGCA AGCTGCCCGT CAGCATCCCC 
CTGGCCAGCG TGGTGCTGCC CAGCCGCGCC GAGAAGGCGA GAAGCACACC CAGCCCTGGG 
CCACCGGCCC GAGAGTCCTC ATCCACGCTG GAGAAGCAGG TGGCTGCTAA CACCCATGGT 
GCCGGGGGCA ACGCTGCTGG GAGCAAGAGC CTCGCCCTGG CTCCCACAGG CTTTGCATAC 
GCCGGCTCGG TGGCCATCAG TGGGGCCCTG GCCGGCAGCC CGGCACCCCT CGCCCCTGGA 
GCCGAGCCCC CAGCCCTGGA CGAGTCCTCC AGCTCTGGAA GCCTCTTTGC CACCGTGGGG 
TCCCGCAGCT CCACCCCGCA GCACCCACCC CTGCTGACGC AGCCACGCAC CTGTGGCTCG 
GCCTCGCCAG CCCCCCAGCT CTGCTCCAGC CCCCGGCTTG GCGCTCTGGG CCCACTCCCC 
GACTCTGGCA AAGGGGACCT GCCCTGCGAG GCCAGCTTCT CAGACCCTGA GAGCGAAGCC 
AAAAGGAGAA TCGTCTTCAC CATCTCGGCG GGCGCCAGCA GCGCCAAGCA GTCACCTTCC 
AGCAAACACA GCCCTCTGCC GTCGGGTGCC CGCGGGGACG GTGGCCAGGG CCATGGGCCA 
GACGGCCGCA AGCGGGGCCG GAGGAAGCGG GCATCGGCAG GGACCCCCAG CCTGAGCTCG 
AGTGTGTCCC CCAAGCGCCG GGCCTTGCCA TCTGTCGCTG GGCTTTTCAC GCAGTCTTCA 
GGGTCCCCCC TGAACCTCAA CTCCATGGTC AGCAACATCA ACCAGCCCCT GGAGATCACA 
GCAATCTCGT CCCCTGAGAG CTCCCTAAAG AGCTCACCCG TCCCCTACCA GGACAACGAC 
CAACCACCCG TGCTCAAGAA GGAGAAGCCC CTGAGCCAGA CCAATGGTGC CCACTACTCC 
CCACTGACTT CGGACGAGGA GCCAGGCTCT GAGGATGAGC CCGGCAGTGC CAGAATTGAG 
AGAAAAATTG CAACGATCTC CTTAGAAAGC AAGTCTCCGC CAAAAACCTT GGAAAATGCA 
GGTGGCAGCC TAGCAGGAAG GAAGGCGCCG CTGGCCAGCG AGCCGGTCAA CAGCAGCAAG 
TGGAAGTCCA CCTTCTCACC CATCTCCGAC CTGGGCCTGG CCAAGGCGGC CGACAGCCCG 
CTGCAGGCTG CCTCTGCTCT GAGCCACAAC TCTCTGTTCG CTTTCCGGCC TGGCCTGGAG 
GAGCCCAGTG CGGCCGATGC CAAGCTGGCC ACCCACTCCA GGAAGAGCTT CCCGGGCACC 
CTGCCAGGGG CGGGCGGGCT GAGCCCCAGT AGCCTTCCTG CCAGCGGCTT CGCCCTGGGC 
GGGGGCCTAG CAGCTGACCT CAGTTTACAC AGCTTCAGTG ATGGTGCTTC TCTCTCCCAC 
AAGGCCCCCG AGGCAGCTGG CCTGGGTGCC CCCCTGAGCT TCCCCGGCCC ACGGGGCAAA 
GAGGGTGGCG CTGCAGAGTC TGGCCCCTTC GTGAACAAGC GGCAGCTGGA CGGACTGGGC 
CCAAAAGGCG AGGGGGGCCT TCCCACATGC GGACCCCCGG ACAAGGCCTC CACAGCGCAT 
AGCAAGGCAG GCAAGGGCCG CGAGCGCGAG CCTGACGTCA AGAACGGCCA CAACCTGTTC 
ATGGCCGCTG CCACTGCGCC CCCTGCAGGC CTCCTCAGCG GCCCAGGTCT TGCCCCAGCG 
GCATCCTCGG CAGGTGGCGC AGCCCCGTCC GTGCAGACCC ACCGCCCCTT CCTGGGCGCC 
TTCGCCCCCG GGCCACAGTT CGCACTGGGC CCCATGTCGC TACAGGCCAA CCTGGGCCCA 
TCTGTGCTGC AGTCCCTGTT CAGTTCCGTG CCCGCCGCCG GCCTGGTTCA CGTCTCGACC 
GCCGCCACCA GATTGACCAA CTCGCACGCC ATGGGCAGCT TCTCCTCTGG GGTGGCCGGC 
GGCGCAGTTG GAGGTGTCTT TAACCACGCG GTGCCTCCCG CCTCCGCCCA TCCGTTTGGA 
GCCAGTTTCG GCAGTGGGGC TGCATGTCGC AGCGCCACGC TGAGCTTAAC CCCGCTGCAG 
GCGGTGGCCA GCACCCCGGC CTCTTCCTTT CAGGCCCCGT CCTCTGCGGA GCCGAGGCCA 
CCCCCACCCC CTCCGCACCT GGGCCGGCCC CCTCCGGGGC AGCCCGCCCT CCACGCACCC 
CCTCATCCTA ACGCCACCTT GCCTCCTCCC CCTGCGCTGC TCCCAGCTAA CTCTGAGCCC 
GTGCTTCTGC AGAACCTCGT ATCCCTCCCT GCTAACCAAG CTTTCTTACC CGCCTCCTCT 
GCCGCCTCTC TGCCGCCTGC TAACGCCTCT CTGTCCATCA AGCTCGCCTC CCTCCCGCAC 
AAGGTGTCCC GGCCCTCCTT GACGGTGCAC CACCAGCCCC TGCCTGGGCT GGCCCTGGCC 
CAGGCCGCGC CCGTGAACCC ACAGGCCAGC TCCACGGGGC CGCCCGCCGT GTGGGTTTCC 
CTTGGCATGC CGCCTCCGTA TGCCGCGCGC CTTGCGGGGG TTAAGCCGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850809.1
CDS	161..5254
Translation	MLSRHAPDSADKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_024995041.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995041.1

ORF Insert Sequence:

ATGCTGAGCC GTCATGCCCC AGACTCGGCT GACAAACACC ACGATGCTGC TCATGAAATC 
ATCGAGACCA TCAGATGGGT CTGTGAAGAA ATCCCGGATC TCAAGCTTGC TATGGAGAAT 
TACGTTTTAA TTGACTACGA TACCAAAAGC TTTGAGAGTA TGCAGAGGCT CTGTGACAAG 
TACAACCGGG CCATCGACAG CATCCACCAG CTGTGGAAGG GCACCACACA GCCCATGAAG 
CTGAACACAC GGCCATCCAC GGGGCTCCTG CGGCACATCC TGCAGCAGGT CTACAACCAC 
TCAGTGACCG ACCCTGAGAA GCTCAACAAC TACGAGCCCT TCTCCCCAGA GGTGTATGGG 
GAGACCTCCT TCGACCTGGT CGCCCAGATG ATCGATGAGA TCAAGATGAC CGAAGATGAC 
CTGTTCGTGG ACCTGGGCAG TGGAGTGGGC CAGGTTGTGC TGCAGGTTGC CGCGGCCACC 
AACTGCAAAC ATCACTACGG TGTAGAGAAA GCTGACATCC CAGCCAAGTA CGCGGAGACC 
ATGGACCGAG AGTTCAGGAA GTGGATGAAA TGGTATGGAA AAAAGCATGC AGAATACACA 
CTGGAGAGGG GGGACTTCCT CTCGGAGGAG TGGAGAGAAC GGATTGCTAA CACAAGTGTT 
GTATTTGTGA ATAACTTTGC CTTTGGTCCT GAGGTGGATC ACCAGCTGAA GGAGCGGTTT 
GCAAACATGA AGGAAGGTGG CAGAATCGTG TCCTCGAAGC CCTTTGCGCC TCTGAACTTC 
AGAATAAACA GTAGAAACTT GAGTGACATC GGCACCATCA TGCGCGTCGT GGAGCTGTCG 
CCGCTAAAAG GCTCGGTGTC ATGGACGGGG AAGCCAGTCT CCTACTACCT GCACACCATC 
GACCGCACCA TACTTGAAAA CTATTTTTCT AGTCTAAAAA ATCCAAAACT CAGGGAGGAA 
CAAGAGGCAG CTCGGCGCCG GCAGCAGCGG GAGAACAAGA GCAACACAAC CACGCCCACC 
AAGGTGCCCG AGAGCAAGGC AGCTGTGCCG GCGGACACCC CCGTGGACTC TGGTGCTGAG 
GAGGAGAAAG CCGGGACGGC CACTGTCAAG AAGCCATCTC CCTCCAAGGC CCGGAAGAAG 
AAGCTGAACA AGAAGGGCCG GAAGATGGTG GGTCGGAAGC GTGGGCGCCC CAAGAAGATG 
AGTGCCACCA ACCCCGAGCG CAAGCCCAAG AAGACCCAGA GCTCACTGGA CCTGCTGCAC 
GCCCAGACCG CGTCACGAGC GGCCTCCCCC TTGCCGCAGG ACGCGCACAG GCCACCTCAC 
AGCCCATTCT ACCAGCTACC TCCCAGCGTG CAGCGGCCTA CCCCCGAGCA GCTGCTGCTG 
GCACCCACCC CGCCCGCACT GCACAGGCTG CTAGAGTCCT TCAGGATTCA GTACCTACAG 
TTCCTGGCAT ACACGAAGAC CCCTCAGTAC AAGGCCAGCC TGCAGCAGCT CCTGGACCAG 
GAGAAGGAGA AGAATGCCCG GTTGCTAGGC GCAGCGCAGC AGCTATTTGG CCACTGCCAA 
GCTCAGAAGG AGGAGATCAA GAGGCTCTTC CAGCAGAAAC TGGACGAGCT GGGAGTGAAG 
GCGCTGACCT ACAACGACCT GATCCAAGCG CAGAAGGAGA TCTCGGCTCA CAACCAGCAG 
TTGAGGGAGC AGACGGAGCA GCTGGAGAAG GGCAACTGGG AGCTGAGGAG CCAGAGCCTG 
AGGCTGCTCA AGGCACGATG CGAGGAGCTG AAGCTGGACT GGTCCACGCT GTCGCTGGAG 
AATCTGCTGA AGGAGAAGCA GGCCCTGAAG AGCCAGATCT CAGAGAAGCA GAGGCACTGC 
CTGGAGCTGC AGATCAGCAT CGTGGAGCTG GAGAAGAGCC AGCGGCAGCA GGAGTTGCTG 
CAGCTCAAGT CCTGCGTGCC GCCTGATGAG GCACTGGCTC TGCAGCTTCG TGGGAAGCCA 
GAGGCTGAGC CTAGCCGGCT GCACTTGGAG CTGGACTGCG CCCGCTTCTC CCTGCCACCC 
TTCAGCAGCT TGAGCCCCGA GCTCTCTATG AATGGCCACG CAGCCGGCTA TGAGCTCTGC 
AGCGCACTGG GTCGGCCCTC GCCCAAGCAG AACACCCCCC AGTACCTGGC CTCCCCGCTA 
GACCAGGAGG TCGTGCCCTG CACCCCTAGC CACAGCGGCC GGCCACGGCT AGAGAAGCTG 
TCCAGCCTGG CCCTGCCTGA CTACACCAGG CTATCCCCTG CCAAGCTGGT GCTGCGGCGC 
CACCTGAGCC AGGACCACGC GGCCAGTGGC AAGGCAGCTG CCAGTGAGCT GCACCCACGA 
GCCGAGCATG CCAAGGAGAA TGGGCTCCCT TACCAGAGCC CTGGCATCAC TAATGGCATC 
AAGCTGAGCC CACAAGACCC CCGACCTTCA TCCCCCACGG CTTTACAGAT GGGAGAGAAG 
GGCCCCGAGA AGGGTGTGAA GGAGCGTGCC TACGCCAGCA GTGGGGAGGC CATCACCAGC 
CTGCCCGTCA GCATCCCGCT GAGCACCGTG CAGCCCAGCA AGCTGCCCGT CAGCATCCCC 
CTGGCCAGCG TGGTGCTGCC CAGCCGCGCC GAGAAGGCGA GAAGCACACC CAGCCCTGGG 
CCACCGGCCC GAGAGTCCTC ATCCACGCTG GAGAAGCAGG TGGCTGCTAA CACCCATGGT 
GCCGGGGGCA ACGCTGCTGG GAGCAAGAGC CTCGCCCTGG CTCCCACAGG CTTTGCATAC 
GCCGGCTCGG TGGCCATCAG TGGGGCCCTG GCCGGCAGCC CGGCACCCCT CGCCCCTGGA 
GCCGAGCCCC CAGCCCTGGA CGAGTCCTCC AGCTCTGGAA GCCTCTTTGC CACCGTGGGG 
TCCCGCAGCT CCACCCCGCA GCACCCACCC CTGCTGACGC AGCCACGCAC CTGTGGCTCG 
GCCTCGCCAG CCCCCCAGCT CTGCTCCAGC CCCCGGCTTG GCGCTCTGGG CCCACTCCCC 
GACTCTGGCA AAGGGGACCT GCCCTGCGAG GCCAGCTTCT CAGACCCTGA GAGCGAAGCC 
AAAAGGAGAA TCGTCTTCAC CATCTCGGCG GGCGCCAGCA GCGCCAAGCA GTCACCTTCC 
AGCAAACACA GCCCTCTGCC GTCGGGTGCC CGCGGGGACG GTGGCCAGGG CCATGGGCCA 
GACGGCCGCA AGCGGGGCCG GAGGAAGCGG GCATCGGCAG GGACCCCCAG CCTGAGCTCG 
AGTGTGTCCC CCAAGCGCCG GGCCTTGCCA TCTGTCGCTG GGCTTTTCAC GCAGTCTTCA 
GGGTCCCCCC TGAACCTCAA CTCCATGGTC AGCAACATCA ACCAGCCCCT GGAGATCACA 
GCAATCTCGT CCCCTGAGAG CTCCCTAAAG AGCTCACCCG TCCCCTACCA GGACAACGAC 
CAACCACCCG TGCTCAAGAA GGAGAAGCCC CTGAGCCAGA CCAATGGTGC CCACTACTCC 
CCACTGACTT CGGACGAGGA GCCAGGCTCT GAGGATGAGC CCGGCAGTGC CAGAATTGAG 
AGAAAAATTG CAACGATCTC CTTAGAAAGC AAGTCTCCGC CAAAAACCTT GGAAAATGCA 
GGTGGCAGCC TAGCAGGAAG GAAGGCGCCG CTGGCCAGCG AGCCGGTCAA CAGCAGCAAG 
TGGAAGTCCA CCTTCTCACC CATCTCCGAC CTGGGCCTGG CCAAGGCGGC CGACAGCCCG 
CTGCAGGCTG CCTCTGCTCT GAGCCACAAC TCTCTGTTCG CTTTCCGGCC TGGCCTGGAG 
GAGCCCAGTG CGGCCGATGC CAAGCTGGCC ACCCACTCCA GGAAGAGCTT CCCGGGCACC 
CTGCCAGGGG CGGGCGGGCT GAGCCCCAGT AGCCTTCCTG CCAGCGGCTT CGCCCTGGGC 
GGGGGCCTAG CAGCTGACCT CAGTTTACAC AGCTTCAGTG ATGGTGCTTC TCTCTCCCAC 
AAGGCCCCCG AGGCAGCTGG CCTGGGTGCC CCCCTGAGCT TCCCCGGCCC ACGGGGCAAA 
GAGGGTGGCG CTGCAGAGTC TGGCCCCTTC GTGAACAAGC GGCAGCTGGA CGGACTGGGC 
CCAAAAGGCG AGGGGGGCCT TCCCACATGC GGACCCCCGG ACAAGGCCTC CACAGCGCAT 
AGCAAGGCAG GCAAGGGCCG CGAGCGCGAG CCTGACGTCA AGAACGGCCA CAACCTGTTC 
ATGGCCGCTG CCACTGCGCC CCCTGCAGGC CTCCTCAGCG GCCCAGGTCT TGCCCCAGCG 
GCATCCTCGG CAGGTGGCGC AGCCCCGTCC GTGCAGACCC ACCGCCCCTT CCTGGGCGCC 
TTCGCCCCCG GGCCACAGTT CGCACTGGGC CCCATGTCGC TACAGGCCAA CCTGGGCCCA 
TCTGTGCTGC AGTCCCTGTT CAGTTCCGTG CCCGCCGCCG GCCTGGTTCA CGTCTCGACC 
GCCGCCACCA GATTGACCAA CTCGCACGCC ATGGGCAGCT TCTCCTCTGG GGTGGCCGGC 
GGCGCAGTTG GAGGTGTCTT TAACCACGCG GTGCCTCCCG CCTCCGCCCA TCCGTTTGGA 
GCCAGTTTCG GCAGTGGGGC TGCATGTCGC AGCGCCACGC TGAGCTTAAC CCCGCTGCAG 
GCGGTGGCCA GCACCCCGGC CTCTTCCTTT CAGGCCCCGT CCTCTGCGGA GCCGAGGCCA 
CCCCCACCCC CTCCGCACCT GGGCCGGCCC CCTCCGGGGC AGCCCGCCCT CCACGCACCC 
CCTCATCCTA ACGCCACCTT GCCTCCTCCC CCTGCGCTGC TCCCAGCTAA CTCTGAGCCC 
GTGCTTCTGC AGAACCTCGT ATCCCTCCCT GCTAACCAAG CTTTCTTACC CGCCTCCTCT 
GCCGCCTCTC TGCCGCCTGC TAACGCCTCT CTGTCCATCA AGCTCGCCTC CCTCCCGCAC 
AAGGTGTCCC GGCCCTCCTT GACGGTGCAC CACCAGCCCC TGCCTGGGCT GGCCCTGGCC 
CAGGCCGCGC CCGTGAACCC ACAGGCCAGC TCCACGGGGC CGCCCGCCGT GTGGGTTTCC 
CTTGGCATGC CGCCTCCGTA TGCCGCGCGC CTTGCGGGGG TTAAGCCGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807068.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805370.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X5, mRNA.

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CloneID	OBa236574
Clone ID Related Accession (Same CDS sequence)	XM_010807068.3
Accession Version	XM_010807068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG 
GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT 
AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG 
GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT 
AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC 
TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA 
CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT 
CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG 
AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC 
GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG 
AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC 
CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG 
TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC 
CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG 
CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC 
ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG 
AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG 
GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC 
AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG 
ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG 
GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG 
GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG 
ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC 
TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT 
AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG 
AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT 
CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC 
GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC 
CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG 
GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC 
AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA 
CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG 
GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC 
ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG 
GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA 
GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC 
GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG 
GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA 
GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC 
ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC 
CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA 
GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC 
GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC 
CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG 
CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC 
AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG 
AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC 
CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG 
CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG 
GACGAGGAGC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA 
ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805370.1
CDS	76..5157
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAELERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807068.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807068.3

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG 
GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT 
AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG 
GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT 
AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC 
TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA 
CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT 
CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG 
AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC 
GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG 
AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC 
CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG 
TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC 
CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG 
CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC 
ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG 
AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG 
GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC 
AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG 
ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG 
GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG 
GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG 
ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC 
TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT 
AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG 
AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT 
CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC 
GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC 
CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG 
GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC 
AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA 
CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG 
GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC 
ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG 
GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA 
GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC 
GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG 
GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA 
GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC 
ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC 
CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA 
GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC 
GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC 
CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG 
CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC 
AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG 
AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC 
CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG 
CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG 
GACGAGGAGC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA 
ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807071.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805373.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X6, mRNA.

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CloneID	OBa236575
Clone ID Related Accession (Same CDS sequence)	XM_010807071.3
Accession Version	XM_010807071.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807071.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG 
CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC 
TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG 
GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC 
GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC 
GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC 
GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA 
GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC 
ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG 
GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT 
CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG 
GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG 
CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC 
AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC 
ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT 
GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC 
CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC 
AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC 
CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG 
CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG 
CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG 
TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC 
CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC 
CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG 
GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC 
AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC 
CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG 
TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG 
AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG 
GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT 
GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC 
CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT 
GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC 
TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA 
GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG 
CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG 
CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT 
AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG 
GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC 
ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC 
AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC 
AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC 
ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC 
TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC 
GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC 
ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC 
TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC 
CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG 
AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG 
TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC 
CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC 
CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG 
CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG 
GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG 
GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC 
CACTACTCCC CACTGACTTC GGACGAGGAG CCAGGCTCTG AGGATGAGCC CGGCAGTGCC 
AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG 
GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC 
AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC 
GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT 
GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC 
CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC 
GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT 
CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA 
CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC 
GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC 
ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC 
AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT 
GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC 
CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC 
CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC 
GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG 
GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT 
CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC 
CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG 
CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC 
CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC 
TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC 
GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC 
CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG 
GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG 
TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805373.1
CDS	173..5155
Translation	MENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807071.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807071.3

ORF Insert Sequence:

ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG 
CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC 
TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG 
GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC 
GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC 
GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC 
GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA 
GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC 
ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG 
GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT 
CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG 
GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG 
CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC 
AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC 
ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT 
GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC 
CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC 
AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC 
CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG 
CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG 
CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG 
TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC 
CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC 
CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG 
GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC 
AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC 
CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG 
TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG 
AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG 
GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT 
GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC 
CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT 
GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC 
TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA 
GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG 
CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG 
CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT 
AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG 
GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC 
ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC 
AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC 
AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC 
ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC 
TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC 
GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC 
ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC 
TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC 
CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG 
AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG 
TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC 
CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC 
CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG 
CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG 
GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG 
GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC 
CACTACTCCC CACTGACTTC GGACGAGGAG CCAGGCTCTG AGGATGAGCC CGGCAGTGCC 
AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG 
GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC 
AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC 
GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT 
GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC 
CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC 
GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT 
CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA 
CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC 
GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC 
ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC 
AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT 
GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC 
CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC 
CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC 
GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG 
GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT 
CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC 
CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG 
CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC 
CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC 
TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC 
GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC 
CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG 
GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG 
TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807072.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805374.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X7, mRNA.

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CloneID	OBa236576
Clone ID Related Accession (Same CDS sequence)	XM_010807072.3
Accession Version	XM_010807072.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG 
TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG 
GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG 
GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805374.1
CDS	76..4860
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGSLPGPPEGGEAVPACPFGSTLRPPPRSFSRNAAVTVVWAEHSGVSLTTRCLPPPPIRLEPVSAVGLHVAAPR

Target ORF information:

RefSeq Version	XM_010807072.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807072.3

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG 
TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG 
GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG 
GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_003586273.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586321.2 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa236577
Clone ID Related Accession (Same CDS sequence)	XM_003586273.5
Accession Version	XM_003586273.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586273.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586321.2
CDS	76..4647
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGN

Target ORF information:

RefSeq Version	XM_003586273.5
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586273.5

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_024995042.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850810.1 Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa236578
Clone ID Related Accession (Same CDS sequence)	XM_024995042.1
Accession Version	XM_024995042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850810.1
CDS	76..4644
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGN

Target ORF information:

RefSeq Version	XM_024995042.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995042.1

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015464181.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319667.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA.

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CloneID	OBa90289
Clone ID Related Accession (Same CDS sequence)	XM_015464181.1 , XM_015472118.1
Accession Version	XM_015464181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAGGC AGTGCCCTCC TGGCCACTTG GTCCCACAGG ACCCGCTGAG GGACGAGCTG 
GGGGCCGCAC GTTGTCTCCG CCACCCACCC AGACTGTTGG GTTTAACCAC CGCTGTTTGC 
AGGTTCCCTT CCAGGGCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319667.1
CDS	65..4768
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGRQCPPGHLVPQDPLRDELGAARCLRHPPRLLGLTTAVCRFPSRAP

Target ORF information:

RefSeq Version	XM_015464181.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464181.1

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAGGC AGTGCCCTCC TGGCCACTTG GTCCCACAGG ACCCGCTGAG GGACGAGCTG 
GGGGCCGCAC GTTGTCTCCG CCACCCACCC AGACTGTTGG GTTTAACCAC CGCTGTTTGC 
AGGTTCCCTT CCAGGGCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015472118.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327604.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA.

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CloneID	OBa90289
Clone ID Related Accession (Same CDS sequence)	XM_015464181.1 , XM_015472118.1
Accession Version	XM_015472118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAGGC AGTGCCCTCC TGGCCACTTG GTCCCACAGG ACCCGCTGAG GGACGAGCTG 
GGGGCCGCAC GTTGTCTCCG CCACCCACCC AGACTGTTGG GTTTAACCAC CGCTGTTTGC 
AGGTTCCCTT CCAGGGCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327604.1
CDS	65..4768
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGRQCPPGHLVPQDPLRDELGAARCLRHPPRLLGLTTAVCRFPSRAP

Target ORF information:

RefSeq Version	XM_015472118.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472118.1

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAGGC AGTGCCCTCC TGGCCACTTG GTCCCACAGG ACCCGCTGAG GGACGAGCTG 
GGGGCCGCAC GTTGTCTCCG CCACCCACCC AGACTGTTGG GTTTAACCAC CGCTGTTTGC 
AGGTTCCCTT CCAGGGCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_003586273.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586321.2 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA.

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CloneID	OBa21246
Clone ID Related Accession (Same CDS sequence)	XM_003586273.4 , XM_015464182.1
Accession Version	XM_003586273.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586273.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586321.2
CDS	64..4635
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGN

Target ORF information:

RefSeq Version	XM_003586273.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586273.4

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015464182.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319668.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa21246
Clone ID Related Accession (Same CDS sequence)	XM_003586273.4 , XM_015464182.1
Accession Version	XM_015464182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319668.1
CDS	64..4635
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGN

Target ORF information:

RefSeq Version	XM_015464182.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464182.1

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015464180.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319666.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA.

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CloneID	OBa47733
Clone ID Related Accession (Same CDS sequence)	XM_015464180.1 , XM_010807072.2
Accession Version	XM_015464180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG 
TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG 
GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG 
GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319666.1
CDS	65..4849
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGSLPGPPEGGEAVPACPFGSTLRPPPRSFSRNAAVTVVWAEHSGVSLTTRCLPPPPIRLEPVSAVGLHVAAPR

Target ORF information:

RefSeq Version	XM_015464180.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464180.1

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG 
TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG 
GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG 
GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807072.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805374.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA.

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CloneID	OBa47733
Clone ID Related Accession (Same CDS sequence)	XM_015464180.1 , XM_010807072.2
Accession Version	XM_010807072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG 
TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG 
GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG 
GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805374.1
CDS	65..4849
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGSLPGPPEGGEAVPACPFGSTLRPPPRSFSRNAAVTVVWAEHSGVSLTTRCLPPPPIRLEPVSAVGLHVAAPR

Target ORF information:

RefSeq Version	XM_010807072.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807072.2

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG 
TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG 
GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG 
GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010798345.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796647.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA.

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CloneID	OBa05346
Clone ID Related Accession (Same CDS sequence)	XM_010798345.2 , XM_010807067.2
Accession Version	XM_010798345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796647.1
CDS	67..5208
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010798345.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798345.2

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807067.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805369.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA.

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CloneID	OBa05346
Clone ID Related Accession (Same CDS sequence)	XM_010798345.2 , XM_010807067.2
Accession Version	XM_010807067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805369.1
CDS	67..5208
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807067.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807067.2

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015464176.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319662.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA.

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CloneID	OBa21243
Clone ID Related Accession (Same CDS sequence)	XM_015464176.1 , XM_005209068.3 , XM_010807066.2 , XM_015464177.1
Accession Version	XM_015464176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319662.1
CDS	67..5211
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_015464176.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464176.1

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_005209068.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209125.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA.

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CloneID	OBa21243
Clone ID Related Accession (Same CDS sequence)	XM_015464176.1 , XM_005209068.3 , XM_010807066.2 , XM_015464177.1
Accession Version	XM_005209068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209125.1
CDS	67..5211
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_005209068.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209068.3

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807066.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805368.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21243
Clone ID Related Accession (Same CDS sequence)	XM_015464176.1 , XM_005209068.3 , XM_010807066.2 , XM_015464177.1
Accession Version	XM_010807066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805368.1
CDS	67..5211
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807066.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807066.2

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015464177.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319663.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA.

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CloneID	OBa21243
Clone ID Related Accession (Same CDS sequence)	XM_015464176.1 , XM_005209068.3 , XM_010807066.2 , XM_015464177.1
Accession Version	XM_015464177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319663.1
CDS	67..5211
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_015464177.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464177.1

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA 
GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG 
GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG 
AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC 
AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA 
GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA 
GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC 
GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA 
GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC 
AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC 
AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC 
GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC 
TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC 
CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA 
TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG 
AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG 
GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC 
TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG 
CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC 
AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG 
AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG 
CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG 
TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG 
CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC 
TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG 
GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG 
GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG 
GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC 
CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT 
GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC 
CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG 
AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC 
AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC 
GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC 
CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC 
AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG 
GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC 
CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC 
TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA 
GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC 
AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA 
ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC 
AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC 
AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC 
CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC 
CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG 
TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC 
GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT 
TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT 
GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC 
CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC 
ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT 
GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT 
GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG 
GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA 
GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC 
GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC 
GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC 
GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA 
GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT 
GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG 
GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC 
GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG 
CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC 
AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT 
GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC 
GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC 
AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC 
CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT 
AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG 
CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT 
CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC 
CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG 
CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG 
CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807071.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805373.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA.

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CloneID	OBa21244
Clone ID Related Accession (Same CDS sequence)	XM_010807071.2 , XM_015464179.1
Accession Version	XM_010807071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG 
CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC 
TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG 
GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC 
GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC 
GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC 
GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA 
GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC 
ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG 
GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT 
CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG 
GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG 
CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC 
AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC 
ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT 
GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC 
CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC 
AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC 
CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG 
CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG 
CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG 
TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC 
CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC 
CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG 
GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC 
AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC 
CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG 
TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG 
AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG 
GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT 
GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC 
CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT 
GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC 
TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA 
GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG 
CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG 
CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT 
AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG 
GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC 
ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC 
AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC 
AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC 
ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC 
TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC 
GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC 
ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC 
TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC 
CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG 
AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG 
TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC 
CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC 
CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG 
CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG 
GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG 
GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC 
CACTACTCCC CACTGACTTC GGACGAGGAA CCAGGCTCTG AGGATGAGCC CGGCAGTGCC 
AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG 
GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC 
AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC 
GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT 
GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC 
CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC 
GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT 
CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA 
CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC 
GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC 
ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC 
AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT 
GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC 
CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC 
CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC 
GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG 
GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT 
CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC 
CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG 
CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC 
CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC 
TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC 
GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC 
CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG 
GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG 
TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805373.1
CDS	154..5136
Translation	MENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807071.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807071.2

ORF Insert Sequence:

ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG 
CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC 
TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG 
GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC 
GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC 
GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC 
GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA 
GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC 
ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG 
GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT 
CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG 
GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG 
CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC 
AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC 
ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT 
GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC 
CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC 
AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC 
CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG 
CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG 
CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG 
TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC 
CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC 
CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG 
GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC 
AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC 
CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG 
TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG 
AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG 
GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT 
GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC 
CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT 
GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC 
TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA 
GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG 
CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG 
CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT 
AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG 
GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC 
ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC 
AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC 
AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC 
ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC 
TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC 
GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC 
ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC 
TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC 
CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG 
AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG 
TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC 
CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC 
CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG 
CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG 
GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG 
GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC 
CACTACTCCC CACTGACTTC GGACGAGGAA CCAGGCTCTG AGGATGAGCC CGGCAGTGCC 
AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG 
GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC 
AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC 
GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT 
GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC 
CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC 
GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT 
CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA 
CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC 
GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC 
ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC 
AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT 
GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC 
CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC 
CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC 
GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG 
GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT 
CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC 
CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG 
CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC 
CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC 
TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC 
GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC 
CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG 
GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG 
TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015464179.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319665.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA.

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CloneID	OBa21244
Clone ID Related Accession (Same CDS sequence)	XM_010807071.2 , XM_015464179.1
Accession Version	XM_015464179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG 
CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC 
TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG 
GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC 
GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC 
GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC 
GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA 
GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC 
ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG 
GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT 
CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG 
GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG 
CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC 
AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC 
ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT 
GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC 
CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC 
AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC 
CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG 
CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG 
CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG 
TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC 
CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC 
CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG 
GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC 
AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC 
CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG 
TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG 
AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG 
GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT 
GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC 
CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT 
GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC 
TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA 
GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG 
CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG 
CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT 
AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG 
GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC 
ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC 
AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC 
AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC 
ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC 
TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC 
GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC 
ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC 
TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC 
CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG 
AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG 
TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC 
CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC 
CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG 
CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG 
GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG 
GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC 
CACTACTCCC CACTGACTTC GGACGAGGAA CCAGGCTCTG AGGATGAGCC CGGCAGTGCC 
AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG 
GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC 
AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC 
GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT 
GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC 
CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC 
GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT 
CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA 
CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC 
GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC 
ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC 
AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT 
GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC 
CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC 
CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC 
GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG 
GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT 
CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC 
CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG 
CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC 
CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC 
TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC 
GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC 
CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG 
GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG 
TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319665.1
CDS	154..5136
Translation	MENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_015464179.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464179.1

ORF Insert Sequence:

ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG 
CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC 
TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG 
GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC 
GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC 
GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC 
GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA 
GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC 
ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG 
GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT 
CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG 
GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG 
CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC 
AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC 
ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT 
GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC 
CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC 
AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC 
CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG 
CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG 
CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG 
TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC 
CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC 
CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG 
GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC 
AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC 
CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG 
TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG 
AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG 
GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT 
GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC 
CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT 
GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC 
TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA 
GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG 
CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG 
CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT 
AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG 
GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC 
ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC 
AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC 
AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC 
ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC 
TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC 
GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC 
ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC 
TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC 
CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG 
AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG 
TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC 
CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC 
CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG 
CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG 
GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG 
GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC 
CACTACTCCC CACTGACTTC GGACGAGGAA CCAGGCTCTG AGGATGAGCC CGGCAGTGCC 
AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG 
GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC 
AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC 
GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT 
GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC 
CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC 
GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT 
CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA 
CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC 
GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC 
ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC 
AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT 
GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC 
CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC 
CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC 
GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG 
GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT 
CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC 
CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG 
CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC 
CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC 
TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC 
GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC 
CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG 
GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG 
TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807073.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805375.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA.

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CloneID	OBa21247
Clone ID Related Accession (Same CDS sequence)	XM_010807073.2 , XM_015464183.1
Accession Version	XM_010807073.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG 
TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC 
TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG 
TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC 
ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG 
GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC 
ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT 
GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG 
AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG 
ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG 
CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT 
CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG 
CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA 
CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC 
CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC 
CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG 
AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG 
CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC 
CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG 
GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC 
TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG 
CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG 
CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA 
CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC 
TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG 
CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG 
CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG 
CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA 
CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC 
ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG 
AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC 
AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC 
CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT 
GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT 
GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA 
TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT 
GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG 
GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC 
TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC 
CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA 
GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT 
TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG 
CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC 
TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT 
TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC 
ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC 
GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC 
TCCCCACTGA CTTCGGACGA GGAACCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT 
GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT 
GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC 
AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC 
CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG 
GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC 
ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG 
GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC 
CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC 
AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG 
GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG 
CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG 
TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA 
GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC 
GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC 
CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG 
ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC 
GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT 
GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG 
CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG 
CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA 
CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG 
CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC 
TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG 
CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG 
GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT 
TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805375.1
CDS	108..4604
Translation	MLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807073.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807073.2

ORF Insert Sequence:

ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG 
TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC 
TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG 
TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC 
ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG 
GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC 
ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT 
GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG 
AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG 
ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG 
CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT 
CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG 
CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA 
CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC 
CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC 
CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG 
AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG 
CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC 
CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG 
GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC 
TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG 
CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG 
CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA 
CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC 
TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG 
CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG 
CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG 
CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA 
CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC 
ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG 
AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC 
AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC 
CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT 
GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT 
GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA 
TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT 
GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG 
GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC 
TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC 
CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA 
GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT 
TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG 
CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC 
TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT 
TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC 
ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC 
GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC 
TCCCCACTGA CTTCGGACGA GGAACCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT 
GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT 
GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC 
AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC 
CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG 
GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC 
ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG 
GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC 
CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC 
AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG 
GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG 
CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG 
TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA 
GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC 
GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC 
CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG 
ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC 
GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT 
GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG 
CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG 
CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA 
CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG 
CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC 
TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG 
CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG 
GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT 
TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015464183.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319669.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa21247
Clone ID Related Accession (Same CDS sequence)	XM_010807073.2 , XM_015464183.1
Accession Version	XM_015464183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG 
TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC 
TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG 
TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC 
ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG 
GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC 
ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT 
GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG 
AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG 
ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG 
CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT 
CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG 
CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA 
CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC 
CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC 
CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG 
AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG 
CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC 
CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG 
GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC 
TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG 
CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG 
CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA 
CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC 
TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG 
CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG 
CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG 
CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA 
CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC 
ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG 
AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC 
AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC 
CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT 
GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT 
GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA 
TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT 
GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG 
GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC 
TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC 
CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA 
GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT 
TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG 
CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC 
TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT 
TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC 
ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC 
GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC 
TCCCCACTGA CTTCGGACGA GGAACCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT 
GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT 
GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC 
AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC 
CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG 
GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC 
ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG 
GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC 
CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC 
AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG 
GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG 
CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG 
TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA 
GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC 
GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC 
CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG 
ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC 
GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT 
GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG 
CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG 
CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA 
CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG 
CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC 
TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG 
CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG 
GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT 
TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319669.1
CDS	108..4604
Translation	MLERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_015464183.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464183.1

ORF Insert Sequence:

ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG 
TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC 
TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG 
TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC 
ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG 
GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC 
ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT 
GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG 
AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG 
ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG 
CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT 
CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG 
CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA 
CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC 
CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC 
CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG 
AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG 
CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC 
CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG 
GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC 
TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG 
CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG 
CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA 
CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC 
TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG 
CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG 
CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG 
CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA 
CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC 
ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG 
AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC 
AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC 
CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT 
GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT 
GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA 
TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT 
GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG 
GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC 
TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC 
CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA 
GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT 
TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG 
CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC 
TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT 
TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC 
ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC 
GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC 
TCCCCACTGA CTTCGGACGA GGAACCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT 
GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT 
GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC 
AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC 
CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG 
GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC 
ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG 
GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC 
CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC 
AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG 
GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG 
CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG 
TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA 
GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC 
GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC 
CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG 
ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC 
GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT 
GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG 
CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG 
CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA 
CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG 
CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC 
TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG 
CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG 
GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT 
TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_015464178.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319664.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa47730
Clone ID Related Accession (Same CDS sequence)	XM_015464178.1 , XM_010807068.2
Accession Version	XM_015464178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG 
GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT 
AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG 
GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT 
AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC 
TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA 
CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT 
CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG 
AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC 
GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG 
AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC 
CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG 
TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC 
CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG 
CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC 
ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG 
AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG 
GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC 
AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG 
ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG 
GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG 
GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG 
ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC 
TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT 
AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG 
AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT 
CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC 
GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC 
CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG 
GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC 
AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA 
CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG 
GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC 
ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG 
GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA 
GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC 
GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG 
GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA 
GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC 
ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC 
CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA 
GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC 
GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC 
CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG 
CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC 
AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG 
AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC 
CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG 
CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG 
GACGAGGAAC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA 
ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319664.1
CDS	67..5148
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAELERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_015464178.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464178.1

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG 
GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT 
AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG 
GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT 
AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC 
TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA 
CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT 
CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG 
AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC 
GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG 
AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC 
CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG 
TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC 
CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG 
CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC 
ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG 
AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG 
GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC 
AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG 
ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG 
GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG 
GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG 
ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC 
TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT 
AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG 
AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT 
CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC 
GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC 
CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG 
GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC 
AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA 
CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG 
GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC 
ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG 
GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA 
GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC 
GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG 
GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA 
GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC 
ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC 
CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA 
GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC 
GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC 
CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG 
CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC 
AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG 
AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC 
CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG 
CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG 
GACGAGGAAC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA 
ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOT1L ( XM_010807068.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805370.1 Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA.

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CloneID	OBa47730
Clone ID Related Accession (Same CDS sequence)	XM_015464178.1 , XM_010807068.2
Accession Version	XM_010807068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG 
GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT 
AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG 
GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT 
AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC 
TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA 
CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT 
CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG 
AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC 
GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG 
AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC 
CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG 
TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC 
CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG 
CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC 
ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG 
AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG 
GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC 
AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG 
ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG 
GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG 
GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG 
ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC 
TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT 
AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG 
AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT 
CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC 
GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC 
CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG 
GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC 
AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA 
CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG 
GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC 
ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG 
GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA 
GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC 
GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG 
GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA 
GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC 
ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC 
CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA 
GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC 
GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC 
CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG 
CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC 
AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG 
AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC 
CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG 
CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG 
GACGAGGAAC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA 
ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805370.1
CDS	67..5148
Translation	MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAELERGDFLSEEWRERIANTSVVFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVPESKAAVPADTPVDSGAEEEKAGTATVKKPSPSKARKKKLNKKGRKMVGRKRGRPKKMSATNPERKPKKTQSSLDLLHAQTASRAASPLPQDAHRPPHSPFYQLPPSVQRPTPEQLLLAPTPPALHRLLESFRIQYLQFLAYTKTPQYKASLQQLLDQEKEKNARLLGAAQQLFGHCQAQKEEIKRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQTEQLEKGNWELRSQSLRLLKARCEELKLDWSTLSLENLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDEALALQLRGKPEAEPSRLHLELDCARFSLPPFSSLSPELSMNGHAAGYELCSALGRPSPKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSSLALPDYTRLSPAKLVLRRHLSQDHAASGKAAASELHPRAEHAKENGLPYQSPGITNGIKLSPQDPRPSSPTALQMGEKGPEKGVKERAYASSGEAITSLPVSIPLSTVQPSKLPVSIPLASVVLPSRAEKARSTPSPGPPARESSSTLEKQVAANTHGAGGNAAGSKSLALAPTGFAYAGSVAISGALAGSPAPLAPGAEPPALDESSSSGSLFATVGSRSSTPQHPPLLTQPRTCGSASPAPQLCSSPRLGALGPLPDSGKGDLPCEASFSDPESEAKRRIVFTISAGASSAKQSPSSKHSPLPSGARGDGGQGHGPDGRKRGRRKRASAGTPSLSSSVSPKRRALPSVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPVPYQDNDQPPVLKKEKPLSQTNGAHYSPLTSDEEPGSEDEPGSARIERKIATISLESKSPPKTLENAGGSLAGRKAPLASEPVNSSKWKSTFSPISDLGLAKAADSPLQAASALSHNSLFAFRPGLEEPSAADAKLATHSRKSFPGTLPGAGGLSPSSLPASGFALGGGLAADLSLHSFSDGASLSHKAPEAAGLGAPLSFPGPRGKEGGAAESGPFVNKRQLDGLGPKGEGGLPTCGPPDKASTAHSKAGKGREREPDVKNGHNLFMAAATAPPAGLLSGPGLAPAASSAGGAAPSVQTHRPFLGAFAPGPQFALGPMSLQANLGPSVLQSLFSSVPAAGLVHVSTAATRLTNSHAMGSFSSGVAGGAVGGVFNHAVPPASAHPFGASFGSGAACRSATLSLTPLQAVASTPASSFQAPSSAEPRPPPPPPHLGRPPPGQPALHAPPHPNATLPPPPALLPANSEPVLLQNLVSLPANQAFLPASSAASLPPANASLSIKLASLPHKVSRPSLTVHHQPLPGLALAQAAPVNPQASSTGPPAVWVSLGMPPPYAARLAGVKPR

Target ORF information:

RefSeq Version	XM_010807068.2
Organism	Bos taurus(cattle)
Definition	Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807068.2

ORF Insert Sequence:

ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC 
ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA 
ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG 
GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA 
CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC 
GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC 
TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG 
GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA 
CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG 
GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT 
AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG 
GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT 
AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC 
TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA 
CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT 
CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG 
AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC 
GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG 
AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC 
CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG 
TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC 
CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG 
CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC 
ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG 
AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG 
GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC 
AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG 
ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG 
GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG 
GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG 
ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC 
TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT 
AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG 
AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT 
CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC 
GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC 
CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG 
GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC 
AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA 
CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG 
GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC 
ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG 
GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA 
GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC 
GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG 
GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA 
GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC 
ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC 
CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA 
GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC 
GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC 
CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG 
CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC 
AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG 
AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC 
CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG 
CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG 
GACGAGGAAC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA 
ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA 
GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC 
TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC 
TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG 
GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG 
GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA 
GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG 
GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT 
GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG 
GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC 
AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC 
ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA 
GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG 
CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG 
TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA 
TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA 
GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC 
AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC 
ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT 
CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC 
GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG 
AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG 
CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG 
CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC 
GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG 
CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DOT1L
Entrez Gene ID	510442
Full Name	DOT1 like histone lysine methyltransferase
General protein information	Preferred Names DOT1 like histone lysine methyltransferase Names histone-lysine N-methyltransferase, H3 lysine-79 specific
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	DOT1L	XP_003953365.1
Canis lupus familiaris (dog)	DOT1L	XP_005633296.1
Homo sapiens (human)	DOT1L	NP_115871.1
	DOT1L	XP_001097213.2
Xenopus tropicalis (tropical clawed frog)	dot1l	XP_002937984.2
Bos taurus (cattle)	DOT1L	XP_005209126.1
Mus musculus (house mouse)	Dot1l	NP_955354.1
Rattus norvegicus (Norway rat)	Dot1l	NP_001102203.1
Gallus gallus (chicken)	DOT1L	XP_004948823.1
Danio rerio (zebrafish)	dot1l	XP_005170590.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DOT1L cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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