EHMT1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following EHMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EHMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220435 XM_024998813.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa220428 XM_024998806.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220429 XM_024998807.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220430 XM_024998808.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220431 XM_024998809.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220432 XM_024998810.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220433 XM_024998811.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220434 XM_024998812.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa36669 NM_001099041.1
Latest version!
Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220435
    Clone ID Related Accession (Same CDS sequence) XM_024998813.1
    Accession Version XM_024998813.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCTGCTG ATGACGGCTC AGCGGAACAG CAGGGAGGGG AGGCCCCGGT GGCTGCAGAC 
    GGGGAGACCA ACGGGTCCTG TGACCAGAGC GATGCCAGTG GCCACGCGGC TGCTGTGAAG
    CACCCTCAGG AGAGCACACG AGGCAGCCCT CAGGAAGGGG CCGGCCCGGG GTCCCGGATA
    GCGGAAAATG GGATTTTGGA CAGAGACCCC GAGGTGGGGA AGCAGAACCA CATCCGCTCT
    GATGGCTTCC CCCAGAATCC TGTCATTGGG AGCAACGGCT ACTTCCTTGG CAAGGCCCCC
    TTGTCTGAGC CACCACTGCG GGTGGCCAGC GCCCTGGCTT CCTCCCTGCC TGGCCACGCG
    GCGAAGACCC TCCCTGGCGG GGCTGGCAAA GGGAGGACTC CCGGTGCTTT TCCCCAGCCG
    CCGGCTGCTG TGCCGGCCAA GCCTGGGGAG GGCAGCAAGG ACACAGAGGA CAAGAAGGCC
    CCGGCCCCGG GCGCCGACGT CAAGGTCCAC AGGGCTCGGA AGACCATGCC GAAGTCCATC
    CCGGGCCTGC ACGCGGCGAG TAAGGACCCC AGGGAAGGCC GGGACGCCCG CGACCACGAG
    GAGCCGAAGG AGGAGCTGAG CAGGGGCGCC TCGGAGTTCG CACGCCAGCA GCTTTCACCC
    CCCTTCCCGT CCCTTCACCA GTCGCTACCT CAGAACCAAT GCTACGTGGC CACCGCGAAG
    GCCCAGACAG CTTGCTTGCC TTTTGTTTTA GCAGCTGCAG TATCTCGGAA GAAAAAGCGA
    AGAATGGGAA CCTATAGCCT GGTTCCTAAG AAAAAGACCA AAGTGTTAAA GCAGAGGACG
    GTGATTGAGA TGTTTAAGAG CATAACTCAT TCCACGGGGG GTTCCAAGGG TGAGAAGGAT
    CTGAGCGATG GCTCCCTCCA TGTGAACGGT GAGAGCCTGG ATCTGGACTC GGAGGAGGAT
    GACTCGGAGG AGCTGGACGA GGATGAGGAC CAGGGCGGCC CACCAGCAGC TGCGTTCCCT
    ACTGAGGATG GCAGGGCCTC CAAGGATGGC GTGTTGGCGC CTGACTGCAG CCAGAAGGTG
    GACGGCGAGT CTGAAGAGGA GCAGGAGTCG GCCGGCACCG CGGACGAGGA GGAGGACGGA
    GACGAGTCCG ACTTGAGTTC TGAGTCCAGC GTCAAGAAGA AGTTCCTCAA GAGGAAGGGG
    AAGCCTGACA GCCCTTGGAT CAAGCCAGCG AGGAAGAGGA GGCGGAGAAG CAAAAAGAAG
    CCCTGCTCTG TGTTAGGTTC TGAAGCCTCT GCGTCGTCTT CGGGCAGTGT GGAGCCAACG
    GCGCCTGGCG ACGGCGGGGG GTACATGGAG GTGCCGCTGG ACTCCTTGAA TCTCCATGTC
    CAGGGTGCGC TGACCTCTGA CACAGAAGGG CTGGCCAACG GTCCTGATGC AGGCGAGACG
    GATGGCCTCC AGGAGGTGCC CCTCTGCAGC TGCCGGATGG AGACCCCAAA GAGCCGGGAG
    ATCACCACTC TGGCCAACAA CCAGTGCATG GCCACAGAGA GTGTGGACCA CCAGCTGGGC
    AGGTGCACGA ACAGCGTGGT GAAGCATGAG CTGATGCGTC CGTCCAGCAA GGCACCGCTG
    CTGGTGCTGT GTGAGGACCA CCGGGGCCGG ATGGTGAAGC ACCAGTGCTG CCCCGGCTGC
    GGCTACTTCT GCACGGCCGG TAATTTTATG GAATGTCAGC CTGAGAGCAG CATTTCTCAT
    CGTTTCCACA AAGCCTGTGC CTCTCGAGTC AATAATGCCA GCTACTGTCC CCACTGTGGG
    GAGGAGACCT CCAAGGCCAA AGAGGTGACC ATTGCAAAGG CAGACACGAC GTCCACGGTG
    ACCCTGGCCC CTGGGCAGGA GAAGAGCTGT GCAGCAGAGG GCAGGGCCGA CACCACCACG
    GGCAGCGCTG CTGGCCCCCT GCTCTCAGAG GAGGACAAGC CGCAGAGCCT GGCCCCCCAG
    GTGCCGGAGG GCTTCGACCC CACAGGGCCG GCTGGGCTGG GGAAGCCGGC GCCAGGCCTC
    TCCCAGGGCC CTGGGAAGGA GACCCTGGAG AGCGCACTCA TCGCCCTGGA TTCCGAGAAA
    CCCAAGAAGC TCCGCTTCCA CCCGAAGCAG CTGTACTTCT CGGCGCGGCA AGGGGAGCTG
    CAGAAGGTGC TGCTCATGTT GGTTGACGGG ATCGACCCCA ACTTCAAGAT GGAGCATCAG
    AACAAGCGCT CGCCCCTGCA CGCCGCGGCG GAGGCTGGCC ACGTGGACAT CTGCCACATG
    CTGATCCAGG CGGGCGCCAA CATCGACACC TGCTCTGAGG ACCAGAGGAC CCCGCTGATG
    GAGGCCGCGG AGAACAACCA CTTGGACGCG GTGAAGTACC TCATCAAGGC CGGGGCCCTC
    GTGGATCCCA AGGCGTGTTA CCTTACAGCT CCCAGAGGGG CAGCAGCCCA GGCTCCAGCG
    GCGCGTGGTG GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854581.1
    CDS73..2547
    Translation

    Target ORF information:

    RefSeq Version XM_024998813.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCTGCTG ATGACGGCTC AGCGGAACAG CAGGGAGGGG AGGCCCCGGT GGCTGCAGAC 
    GGGGAGACCA ACGGGTCCTG TGACCAGAGC GATGCCAGTG GCCACGCGGC TGCTGTGAAG
    CACCCTCAGG AGAGCACACG AGGCAGCCCT CAGGAAGGGG CCGGCCCGGG GTCCCGGATA
    GCGGAAAATG GGATTTTGGA CAGAGACCCC GAGGTGGGGA AGCAGAACCA CATCCGCTCT
    GATGGCTTCC CCCAGAATCC TGTCATTGGG AGCAACGGCT ACTTCCTTGG CAAGGCCCCC
    TTGTCTGAGC CACCACTGCG GGTGGCCAGC GCCCTGGCTT CCTCCCTGCC TGGCCACGCG
    GCGAAGACCC TCCCTGGCGG GGCTGGCAAA GGGAGGACTC CCGGTGCTTT TCCCCAGCCG
    CCGGCTGCTG TGCCGGCCAA GCCTGGGGAG GGCAGCAAGG ACACAGAGGA CAAGAAGGCC
    CCGGCCCCGG GCGCCGACGT CAAGGTCCAC AGGGCTCGGA AGACCATGCC GAAGTCCATC
    CCGGGCCTGC ACGCGGCGAG TAAGGACCCC AGGGAAGGCC GGGACGCCCG CGACCACGAG
    GAGCCGAAGG AGGAGCTGAG CAGGGGCGCC TCGGAGTTCG CACGCCAGCA GCTTTCACCC
    CCCTTCCCGT CCCTTCACCA GTCGCTACCT CAGAACCAAT GCTACGTGGC CACCGCGAAG
    GCCCAGACAG CTTGCTTGCC TTTTGTTTTA GCAGCTGCAG TATCTCGGAA GAAAAAGCGA
    AGAATGGGAA CCTATAGCCT GGTTCCTAAG AAAAAGACCA AAGTGTTAAA GCAGAGGACG
    GTGATTGAGA TGTTTAAGAG CATAACTCAT TCCACGGGGG GTTCCAAGGG TGAGAAGGAT
    CTGAGCGATG GCTCCCTCCA TGTGAACGGT GAGAGCCTGG ATCTGGACTC GGAGGAGGAT
    GACTCGGAGG AGCTGGACGA GGATGAGGAC CAGGGCGGCC CACCAGCAGC TGCGTTCCCT
    ACTGAGGATG GCAGGGCCTC CAAGGATGGC GTGTTGGCGC CTGACTGCAG CCAGAAGGTG
    GACGGCGAGT CTGAAGAGGA GCAGGAGTCG GCCGGCACCG CGGACGAGGA GGAGGACGGA
    GACGAGTCCG ACTTGAGTTC TGAGTCCAGC GTCAAGAAGA AGTTCCTCAA GAGGAAGGGG
    AAGCCTGACA GCCCTTGGAT CAAGCCAGCG AGGAAGAGGA GGCGGAGAAG CAAAAAGAAG
    CCCTGCTCTG TGTTAGGTTC TGAAGCCTCT GCGTCGTCTT CGGGCAGTGT GGAGCCAACG
    GCGCCTGGCG ACGGCGGGGG GTACATGGAG GTGCCGCTGG ACTCCTTGAA TCTCCATGTC
    CAGGGTGCGC TGACCTCTGA CACAGAAGGG CTGGCCAACG GTCCTGATGC AGGCGAGACG
    GATGGCCTCC AGGAGGTGCC CCTCTGCAGC TGCCGGATGG AGACCCCAAA GAGCCGGGAG
    ATCACCACTC TGGCCAACAA CCAGTGCATG GCCACAGAGA GTGTGGACCA CCAGCTGGGC
    AGGTGCACGA ACAGCGTGGT GAAGCATGAG CTGATGCGTC CGTCCAGCAA GGCACCGCTG
    CTGGTGCTGT GTGAGGACCA CCGGGGCCGG ATGGTGAAGC ACCAGTGCTG CCCCGGCTGC
    GGCTACTTCT GCACGGCCGG TAATTTTATG GAATGTCAGC CTGAGAGCAG CATTTCTCAT
    CGTTTCCACA AAGCCTGTGC CTCTCGAGTC AATAATGCCA GCTACTGTCC CCACTGTGGG
    GAGGAGACCT CCAAGGCCAA AGAGGTGACC ATTGCAAAGG CAGACACGAC GTCCACGGTG
    ACCCTGGCCC CTGGGCAGGA GAAGAGCTGT GCAGCAGAGG GCAGGGCCGA CACCACCACG
    GGCAGCGCTG CTGGCCCCCT GCTCTCAGAG GAGGACAAGC CGCAGAGCCT GGCCCCCCAG
    GTGCCGGAGG GCTTCGACCC CACAGGGCCG GCTGGGCTGG GGAAGCCGGC GCCAGGCCTC
    TCCCAGGGCC CTGGGAAGGA GACCCTGGAG AGCGCACTCA TCGCCCTGGA TTCCGAGAAA
    CCCAAGAAGC TCCGCTTCCA CCCGAAGCAG CTGTACTTCT CGGCGCGGCA AGGGGAGCTG
    CAGAAGGTGC TGCTCATGTT GGTTGACGGG ATCGACCCCA ACTTCAAGAT GGAGCATCAG
    AACAAGCGCT CGCCCCTGCA CGCCGCGGCG GAGGCTGGCC ACGTGGACAT CTGCCACATG
    CTGATCCAGG CGGGCGCCAA CATCGACACC TGCTCTGAGG ACCAGAGGAC CCCGCTGATG
    GAGGCCGCGG AGAACAACCA CTTGGACGCG GTGAAGTACC TCATCAAGGC CGGGGCCCTC
    GTGGATCCCA AGGCGTGTTA CCTTACAGCT CCCAGAGGGG CAGCAGCCCA GGCTCCAGCG
    GCGCGTGGTG GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220428
    Clone ID Related Accession (Same CDS sequence) XM_024998806.1
    Accession Version XM_024998806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTGCTG ATGACGGCTC AGCGGAACAG CAGGGAGGGG AGGCCCCGGT GGCTGCAGAC 
    GGGGAGACCA ACGGGTCCTG TGACCAGAGC GATGCCAGTG GCCACGCGGC TGCTGTGAAG
    CACCCTCAGG AGAGCACACG AGGCAGCCCT CAGGAAGGGG CCGGCCCGGG GTCCCGGATA
    GCGGAAAATG GGATTTTGGA CAGAGACCCC GAGGTGGGGA AGCAGAACCA CATCCGCTCT
    GATGGCTTCC CCCAGAATCC TGTCATTGGG AGCAACGGCT ACTTCCTTGG CAAGGCCCCC
    TTGTCTGAGC CACCACTGCG GGTGGCCAGC GCCCTGGCTT CCTCCCTGCC TGGCCACGCG
    GCGAAGACCC TCCCTGGCGG GGCTGGCAAA GGGAGGACTC CCGGTGCTTT TCCCCAGCCG
    CCGGCTGCTG TGCCGGCCAA GCCTGGGGAG GGCAGCAAGG ACACAGAGGA CAAGAAGGCC
    CCGGCCCCGG GCGCCGACGT CAAGGTCCAC AGGGCTCGGA AGACCATGCC GAAGTCCATC
    CCGGGCCTGC ACGCGGCGAG TAAGGACCCC AGGGAAGGCC GGGACGCCCG CGACCACGAG
    GAGCCGAAGG AGGAGCTGAG CAGGGGCGCC TCGGAGTTCG CACGCCAGCA GCTTTCACCC
    CCCTTCCCGT CCCTTCACCA GTCGCTACCT CAGAACCAAT GCTACGTGGC CACCGCGAAG
    GCCCAGACAG CTGCAGTATC TCGGAAGAAA AAGCGAAGAA TGGGAACCTA TAGCCTGGTT
    CCTAAGAAAA AGACCAAAGT GTTAAAGCAG AGGACGGTGA TTGAGATGTT TAAGAGCATA
    ACTCATTCCA CGGGGGGTTC CAAGGGTGAG AAGGATCTGA GCGATGGCTC CCTCCATGTG
    AACGGTGAGA GCCTGGATCT GGACTCGGAG GAGGATGACT CGGAGGAGCT GGACGAGGAT
    GAGGACCAGG GCGGCCCACC AGCAGCTGCG TTCCCTACTG AGGATGGCAG GGCCTCCAAG
    GATGGCGTGT TGGCGCCTGA CTGCAGCCAG AAGGTGGACG GCGAGTCTGA AGAGGAGCAG
    GAGTCGGCCG GCACCGCGGA CGAGGAGGAG GACGGAGACG AGTCCGACTT GAGTTCTGAG
    TCCAGCGTCA AGAAGAAGTT CCTCAAGAGG AAGGGGAAGC CTGACAGCCC TTGGATCAAG
    CCAGCGAGGA AGAGGAGGCG GAGAAGCAAA AAGAAGCCCT GCTCTGTGTT AGGTTCTGAA
    GCCTCTGCGT CGTCTTCGGG CAGTGTGGAG CCAACGGCGC CTGGCGACGG CGGGGGGTAC
    ATGGAGGTGC CGCTGGACTC CTTGAATCTC CATGTCCAGG GTGCGCTGAC CTCTGACACA
    GAAGGGCTGG CCAACGGTCC TGATGCAGGC GAGACGGATG GCCTCCAGGA GGTGCCCCTC
    TGCAGCTGCC GGATGGAGAC CCCAAAGAGC CGGGAGATCA CCACTCTGGC CAACAACCAG
    TGCATGGCCA CAGAGAGTGT GGACCACCAG CTGGGCAGGT GCACGAACAG CGTGGTGAAG
    CATGAGCTGA TGCGTCCGTC CAGCAAGGCA CCGCTGCTGG TGCTGTGTGA GGACCACCGG
    GGCCGGATGG TGAAGCACCA GTGCTGCCCC GGCTGCGGCT ACTTCTGCAC GGCCGGTAAT
    TTTATGGAAT GTCAGCCTGA GAGCAGCATT TCTCATCGTT TCCACAAAGC CTGTGCCTCT
    CGAGTCAATA ATGCCAGCTA CTGTCCCCAC TGTGGGGAGG AGACCTCCAA GGCCAAAGAG
    GTGACCATTG CAAAGGCAGA CACGACGTCC ACGGTGACCC TGGCCCCTGG GCAGGAGAAG
    AGCTGTGCAG CAGAGGGCAG GGCCGACACC ACCACGGGCA GCGCTGCTGG CCCCCTGCTC
    TCAGAGGAGG ACAAGCCGCA GAGCCTGGCC CCCCAGGTGC CGGAGGGCTT CGACCCCACA
    GGGCCGGCTG GGCTGGGGAA GCCGGCGCCA GGCCTCTCCC AGGGCCCTGG GAAGGAGACC
    CTGGAGAGCG CACTCATCGC CCTGGATTCC GAGAAACCCA AGAAGCTCCG CTTCCACCCG
    AAGCAGCTGT ACTTCTCGGC GCGGCAAGGG GAGCTGCAGA AGGTGCTGCT CATGTTGGTT
    GACGGGATCG ACCCCAACTT CAAGATGGAG CATCAGAACA AGCGCTCGCC CCTGCACGCC
    GCGGCGGAGG CTGGCCACGT GGACATCTGC CACATGCTGA TCCAGGCGGG CGCCAACATC
    GACACCTGCT CTGAGGACCA GAGGACCCCG CTGATGGAGG CCGCGGAGAA CAACCACTTG
    GACGCGGTGA AGTACCTCAT CAAGGCCGGG GCCCTCGTGG ATCCCAAGGA CGCAGAGGGC
    TCGACCTGTT TGCACCTGGC TGCCAAGAAA GGCCACTACG ACGTGGTTCA GTACTTGCTC
    TCAAACGGAC AGATGGACGT CAACTGTCAG GATGATGGTG GCTGGACGCC CATGATCTGG
    GCCACTGAGT ACAAGCACGT GGACCTCGTG AAGCTGTTGC TCTCCAAGGG GTCTGACATC
    AACATCCGGG ACAACGAGGA AAACATCTGC CTGCACTGGG CGGCCTTCTC AGGCTGTGTG
    GACATCGCTG AGATCCTGCT GGCCGCCAGG TGCGACCTGC ACGCGGTGAA CATCCACGGG
    GACTCGCCCC TGCACATCGC GGCCCGGGAG GACCGCTACG CCTGTGTCGT CCTCTTCCTG
    TCCCGGGACT CGGATGTCAC TCTGAAGAAC AAGGAGGGGG AGACTCCCCT GCAGTGCGCC
    AGCCTCAACT CACAGGTGTG GAGCGCCCTG CAGGTGAGCC AGGCGCTGCG GGACGCGGCC
    CCTGACAGGC CCACCCCGGT GGAGAAGACG GTGAGCAGGG ACATTGCTCG GGGCTACGAG
    CGGATCCCCA TCCCCTGCGT CAATGGTGTG GACGGCGAGC CGTGCCCCAG CAACTACAAG
    TACGTGTCTC AGAACTGTGT GACGTCCCCC ATGAACATCG ATAGGAACAT CACCCACCTG
    CAGTACTGCG TGTGCATCGA CGACTGCTCC TCCAGCAACT GCATGTGTGG CCAGCTCAGC
    ATGCGCTGCT GGTACGACAA GGACGGTCGG CTGCTGCCAG AGTTCAACAT GGCAGAGCCG
    CCCCTGCTGT TCGAGTGCAA CCACGCCTGC TCCTGCTGGA GGACGTGCCG CAACCGCGTG
    GTGCAGAATG GCCTCAGGGC GAGGCTGCAG CTCTATCGGA CACAGAACAT GGGCTGGGGC
    GTCCGGTCCC TGCAGGACAT CCCACTGGGC ACCTTTGTTT GCGAGTACGT TGGGGAGCTT
    ATTTCTGACT CGGAAGCTGA TGTGCGGGAA GAAGACTCTT ATCTCTTTGA TCTTGACAAC
    AAGGACGGAG AGCTGTACTG CATCGACGCG CGTTTCTATG GCAACGTCAG CCGCTTCATC
    AACCACCACT GTGAGCCCAA TCTGGTGCCC GTGCGTGTGT TCATGTCCCA CCAGGACCTG
    CGCTTCCCGC GGATCGCCTT CTTCAGCACC CGCCTGATTG AGGCCGGGGA GCAGCTGGGC
    TTCGACTATG GGCAGCGCTT CTGGGACATC AAGGGCAAGC TGTTCAGCTG CCGCTGCGGC
    TCCCCCAAGT GCCGGCACTC CGGGGCCGCG CTGGCCCAGC GGCAGGCCGG TGCCCAGGAG
    GACGGGCTGC CCGACACCAG CTCCTCGCCT GCCGACCCGC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854574.1
    CDS73..3837
    Translation

    Target ORF information:

    RefSeq Version XM_024998806.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTGCTG ATGACGGCTC AGCGGAACAG CAGGGAGGGG AGGCCCCGGT GGCTGCAGAC 
    GGGGAGACCA ACGGGTCCTG TGACCAGAGC GATGCCAGTG GCCACGCGGC TGCTGTGAAG
    CACCCTCAGG AGAGCACACG AGGCAGCCCT CAGGAAGGGG CCGGCCCGGG GTCCCGGATA
    GCGGAAAATG GGATTTTGGA CAGAGACCCC GAGGTGGGGA AGCAGAACCA CATCCGCTCT
    GATGGCTTCC CCCAGAATCC TGTCATTGGG AGCAACGGCT ACTTCCTTGG CAAGGCCCCC
    TTGTCTGAGC CACCACTGCG GGTGGCCAGC GCCCTGGCTT CCTCCCTGCC TGGCCACGCG
    GCGAAGACCC TCCCTGGCGG GGCTGGCAAA GGGAGGACTC CCGGTGCTTT TCCCCAGCCG
    CCGGCTGCTG TGCCGGCCAA GCCTGGGGAG GGCAGCAAGG ACACAGAGGA CAAGAAGGCC
    CCGGCCCCGG GCGCCGACGT CAAGGTCCAC AGGGCTCGGA AGACCATGCC GAAGTCCATC
    CCGGGCCTGC ACGCGGCGAG TAAGGACCCC AGGGAAGGCC GGGACGCCCG CGACCACGAG
    GAGCCGAAGG AGGAGCTGAG CAGGGGCGCC TCGGAGTTCG CACGCCAGCA GCTTTCACCC
    CCCTTCCCGT CCCTTCACCA GTCGCTACCT CAGAACCAAT GCTACGTGGC CACCGCGAAG
    GCCCAGACAG CTGCAGTATC TCGGAAGAAA AAGCGAAGAA TGGGAACCTA TAGCCTGGTT
    CCTAAGAAAA AGACCAAAGT GTTAAAGCAG AGGACGGTGA TTGAGATGTT TAAGAGCATA
    ACTCATTCCA CGGGGGGTTC CAAGGGTGAG AAGGATCTGA GCGATGGCTC CCTCCATGTG
    AACGGTGAGA GCCTGGATCT GGACTCGGAG GAGGATGACT CGGAGGAGCT GGACGAGGAT
    GAGGACCAGG GCGGCCCACC AGCAGCTGCG TTCCCTACTG AGGATGGCAG GGCCTCCAAG
    GATGGCGTGT TGGCGCCTGA CTGCAGCCAG AAGGTGGACG GCGAGTCTGA AGAGGAGCAG
    GAGTCGGCCG GCACCGCGGA CGAGGAGGAG GACGGAGACG AGTCCGACTT GAGTTCTGAG
    TCCAGCGTCA AGAAGAAGTT CCTCAAGAGG AAGGGGAAGC CTGACAGCCC TTGGATCAAG
    CCAGCGAGGA AGAGGAGGCG GAGAAGCAAA AAGAAGCCCT GCTCTGTGTT AGGTTCTGAA
    GCCTCTGCGT CGTCTTCGGG CAGTGTGGAG CCAACGGCGC CTGGCGACGG CGGGGGGTAC
    ATGGAGGTGC CGCTGGACTC CTTGAATCTC CATGTCCAGG GTGCGCTGAC CTCTGACACA
    GAAGGGCTGG CCAACGGTCC TGATGCAGGC GAGACGGATG GCCTCCAGGA GGTGCCCCTC
    TGCAGCTGCC GGATGGAGAC CCCAAAGAGC CGGGAGATCA CCACTCTGGC CAACAACCAG
    TGCATGGCCA CAGAGAGTGT GGACCACCAG CTGGGCAGGT GCACGAACAG CGTGGTGAAG
    CATGAGCTGA TGCGTCCGTC CAGCAAGGCA CCGCTGCTGG TGCTGTGTGA GGACCACCGG
    GGCCGGATGG TGAAGCACCA GTGCTGCCCC GGCTGCGGCT ACTTCTGCAC GGCCGGTAAT
    TTTATGGAAT GTCAGCCTGA GAGCAGCATT TCTCATCGTT TCCACAAAGC CTGTGCCTCT
    CGAGTCAATA ATGCCAGCTA CTGTCCCCAC TGTGGGGAGG AGACCTCCAA GGCCAAAGAG
    GTGACCATTG CAAAGGCAGA CACGACGTCC ACGGTGACCC TGGCCCCTGG GCAGGAGAAG
    AGCTGTGCAG CAGAGGGCAG GGCCGACACC ACCACGGGCA GCGCTGCTGG CCCCCTGCTC
    TCAGAGGAGG ACAAGCCGCA GAGCCTGGCC CCCCAGGTGC CGGAGGGCTT CGACCCCACA
    GGGCCGGCTG GGCTGGGGAA GCCGGCGCCA GGCCTCTCCC AGGGCCCTGG GAAGGAGACC
    CTGGAGAGCG CACTCATCGC CCTGGATTCC GAGAAACCCA AGAAGCTCCG CTTCCACCCG
    AAGCAGCTGT ACTTCTCGGC GCGGCAAGGG GAGCTGCAGA AGGTGCTGCT CATGTTGGTT
    GACGGGATCG ACCCCAACTT CAAGATGGAG CATCAGAACA AGCGCTCGCC CCTGCACGCC
    GCGGCGGAGG CTGGCCACGT GGACATCTGC CACATGCTGA TCCAGGCGGG CGCCAACATC
    GACACCTGCT CTGAGGACCA GAGGACCCCG CTGATGGAGG CCGCGGAGAA CAACCACTTG
    GACGCGGTGA AGTACCTCAT CAAGGCCGGG GCCCTCGTGG ATCCCAAGGA CGCAGAGGGC
    TCGACCTGTT TGCACCTGGC TGCCAAGAAA GGCCACTACG ACGTGGTTCA GTACTTGCTC
    TCAAACGGAC AGATGGACGT CAACTGTCAG GATGATGGTG GCTGGACGCC CATGATCTGG
    GCCACTGAGT ACAAGCACGT GGACCTCGTG AAGCTGTTGC TCTCCAAGGG GTCTGACATC
    AACATCCGGG ACAACGAGGA AAACATCTGC CTGCACTGGG CGGCCTTCTC AGGCTGTGTG
    GACATCGCTG AGATCCTGCT GGCCGCCAGG TGCGACCTGC ACGCGGTGAA CATCCACGGG
    GACTCGCCCC TGCACATCGC GGCCCGGGAG GACCGCTACG CCTGTGTCGT CCTCTTCCTG
    TCCCGGGACT CGGATGTCAC TCTGAAGAAC AAGGAGGGGG AGACTCCCCT GCAGTGCGCC
    AGCCTCAACT CACAGGTGTG GAGCGCCCTG CAGGTGAGCC AGGCGCTGCG GGACGCGGCC
    CCTGACAGGC CCACCCCGGT GGAGAAGACG GTGAGCAGGG ACATTGCTCG GGGCTACGAG
    CGGATCCCCA TCCCCTGCGT CAATGGTGTG GACGGCGAGC CGTGCCCCAG CAACTACAAG
    TACGTGTCTC AGAACTGTGT GACGTCCCCC ATGAACATCG ATAGGAACAT CACCCACCTG
    CAGTACTGCG TGTGCATCGA CGACTGCTCC TCCAGCAACT GCATGTGTGG CCAGCTCAGC
    ATGCGCTGCT GGTACGACAA GGACGGTCGG CTGCTGCCAG AGTTCAACAT GGCAGAGCCG
    CCCCTGCTGT TCGAGTGCAA CCACGCCTGC TCCTGCTGGA GGACGTGCCG CAACCGCGTG
    GTGCAGAATG GCCTCAGGGC GAGGCTGCAG CTCTATCGGA CACAGAACAT GGGCTGGGGC
    GTCCGGTCCC TGCAGGACAT CCCACTGGGC ACCTTTGTTT GCGAGTACGT TGGGGAGCTT
    ATTTCTGACT CGGAAGCTGA TGTGCGGGAA GAAGACTCTT ATCTCTTTGA TCTTGACAAC
    AAGGACGGAG AGCTGTACTG CATCGACGCG CGTTTCTATG GCAACGTCAG CCGCTTCATC
    AACCACCACT GTGAGCCCAA TCTGGTGCCC GTGCGTGTGT TCATGTCCCA CCAGGACCTG
    CGCTTCCCGC GGATCGCCTT CTTCAGCACC CGCCTGATTG AGGCCGGGGA GCAGCTGGGC
    TTCGACTATG GGCAGCGCTT CTGGGACATC AAGGGCAAGC TGTTCAGCTG CCGCTGCGGC
    TCCCCCAAGT GCCGGCACTC CGGGGCCGCG CTGGCCCAGC GGCAGGCCGG TGCCCAGGAG
    GACGGGCTGC CCGACACCAG CTCCTCGCCT GCCGACCCGC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220429
    Clone ID Related Accession (Same CDS sequence) XM_024998807.1
    Accession Version XM_024998807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCTTGCTT GCCTTTTGTT
    TTAGCAGCTG CAGTATCTCG GAAGAAAAAG CGAAGAATGG GAACCTATAG CCTGGTTCCT
    AAGAAAAAGA CCAAAGTGTT AAAGCAGAGG ACGGTGATTG AGATGTTTAA GAGCATAACT
    CATTCCACGG GGGGTTCCAA GGGTGAGAAG GATCTGAGCG ATGGCTCCCT CCATGTGAAC
    GGTGAGAGCC TGGATCTGGA CTCGGAGGAG GATGACTCGG AGGAGCTGGA CGAGGATGAG
    GACCAGGGCG GCCCACCAGC AGCTGCGTTC CCTACTGAGG ATGGCAGGGC CTCCAAGGAT
    GGCGTGTTGG CGCCTGACTG CAGCCAGAAG GTGGACGGCG AGTCTGAAGA GGAGCAGGAG
    TCGGCCGGCA CCGCGGACGA GGAGGAGGAC GGAGACGAGT CCGACTTGAG TTCTGAGTCC
    AGCGTCAAGA AGAAGTTCCT CAAGAGGAAG GGGAAGCCTG ACAGCCCTTG GATCAAGCCA
    GCGAGGAAGA GGAGGCGGAG AAGCAAAAAG AAGCCCTGCT CTGTGTTAGG TTCTGAAGCC
    TCTGCGTCGT CTTCGGGCAG TGTGGAGCCA ACGGCGCCTG GCGACGGCGG GGGGTACATG
    GAGGTGCCGC TGGACTCCTT GAATCTCCAT GTCCAGGGTG CGCTGACCTC TGACACAGAA
    GGGCTGGCCA ACGGTCCTGA TGCAGGCGAG ACGGATGGCC TCCAGGAGGT GCCCCTCTGC
    AGCTGCCGGA TGGAGACCCC AAAGAGCCGG GAGATCACCA CTCTGGCCAA CAACCAGTGC
    ATGGCCACAG AGAGTGTGGA CCACCAGCTG GGCAGGTGCA CGAACAGCGT GGTGAAGCAT
    GAGCTGATGC GTCCGTCCAG CAAGGCACCG CTGCTGGTGC TGTGTGAGGA CCACCGGGGC
    CGGATGGTGA AGCACCAGTG CTGCCCCGGC TGCGGCTACT TCTGCACGGC CGGTAATTTT
    ATGGAATGTC AGCCTGAGAG CAGCATTTCT CATCGTTTCC ACAAAGCCTG TGCCTCTCGA
    GTCAATAATG CCAGCTACTG TCCCCACTGT GGGGAGGAGA CCTCCAAGGC CAAAGAGGTG
    ACCATTGCAA AGGCAGACAC GACGTCCACG GTGACCCTGG CCCCTGGGCA GGAGAAGAGC
    TGTGCAGCAG AGGGCAGGGC CGACACCACC ACGGGCAGCG CTGCTGGCCC CCTGCTCTCA
    GAGGAGGACA AGCCGCAGAG CCTGGCCCCC CAGGTGCCGG AGGGCTTCGA CCCCACAGGG
    CCGGCTGGGC TGGGGAAGCC GGCGCCAGGC CTCTCCCAGG GCCCTGGGAA GGAGACCCTG
    GAGAGCGCAC TCATCGCCCT GGATTCCGAG AAACCCAAGA AGCTCCGCTT CCACCCGAAG
    CAGCTGTACT TCTCGGCGCG GCAAGGGGAG CTGCAGAAGG TGCTGCTCAT GTTGGTTGAC
    GGGATCGACC CCAACTTCAA GATGGAGCAT CAGAACAAGC GCTCGCCCCT GCACGCCGCG
    GCGGAGGCTG GCCACGTGGA CATCTGCCAC ATGCTGATCC AGGCGGGCGC CAACATCGAC
    ACCTGCTCTG AGGACCAGAG GACCCCGCTG ATGGAGGCCG CGGAGAACAA CCACTTGGAC
    GCGGTGAAGT ACCTCATCAA GGCCGGGGCC CTCGTGGATC CCAAGGACGC AGAGGGCTCG
    ACCTGTTTGC ACCTGGCTGC CAAGAAAGGC CACTACGACG TGGTTCAGTA CTTGCTCTCA
    AACGGACAGA TGGACGTCAA CTGTCAGGAT GATGGTGGCT GGACGCCCAT GATCTGGGCC
    ACTGAGTACA AGCACGTGGA CCTCGTGAAG CTGTTGCTCT CCAAGGGGTC TGACATCAAC
    ATCCGGGACA ACGAGGAAAA CATCTGCCTG CACTGGGCGG CCTTCTCAGG CTGTGTGGAC
    ATCGCTGAGA TCCTGCTGGC CGCCAGGTGC GACCTGCACG CGGTGAACAT CCACGGGGAC
    TCGCCCCTGC ACATCGCGGC CCGGGAGGAC CGCTACGCCT GTGTCGTCCT CTTCCTGTCC
    CGGGACTCGG ATGTCACTCT GAAGAACAAG GAGGGGGAGA CTCCCCTGCA GTGCGCCAGC
    CTCAACTCAC AGGTGTGGAG CGCCCTGCAG GTGAGCCAGG CGCTGCGGGA CGCGGCCCCT
    GACAGGCCCA CCCCGGTGGA GAAGACGGTG AGCAGGGACA TTGCTCGGGG CTACGAGCGG
    ATCCCCATCC CCTGCGTCAA TGGTGTGGAC GGCGAGCCGT GCCCCAGCAA CTACAAGTAC
    GTGTCTCAGA ACTGTGTGAC GTCCCCCATG AACATCGATA GGAACATCAC CCACCTGCAG
    TACTGCGTGT GCATCGACGA CTGCTCCTCC AGCAACTGCA TGTGTGGCCA GCTCAGCATG
    CGCTGCTGGT ACGACAAGGA CGGTCGGCTG CTGCCAGAGT TCAACATGGC AGAGCCGCCC
    CTGCTGTTCG AGTGCAACCA CGCCTGCTCC TGCTGGAGGA CGTGCCGCAA CCGCGTGGTG
    CAGAATGGCC TCAGGGCGAG GCTGCAGCTC TATCGGACAC AGAACATGGG CTGGGGCGTC
    CGGTCCCTGC AGGACATCCC ACTGGGCACC TTTGTTTGCG AGTACGTTGG GGAGCTTATT
    TCTGACTCGG AAGCTGATGT GCGGGAAGAA GACTCTTATC TCTTTGATCT TGACAACAAG
    GACGGAGAGC TGTACTGCAT CGACGCGCGT TTCTATGGCA ACGTCAGCCG CTTCATCAAC
    CACCACTGTG AGCCCAATCT GGTGCCCGTG CGTGTGTTCA TGTCCCACCA GGACCTGCGC
    TTCCCGCGGA TCGCCTTCTT CAGCACCCGC CTGATTGAGG CCGGGGAGCA GCTGGGCTTC
    GACTATGGGC AGCGCTTCTG GGACATCAAG GGCAAGCTGT TCAGCTGCCG CTGCGGCTCC
    CCCAAGTGCC GGCACTCCGG GGCCGCGCTG GCCCAGCGGC AGGCCGGTGC CCAGGAGGAC
    GGGCTGCCCG ACACCAGCTC CTCGCCTGCC GACCCGCTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854575.1
    CDS42..3923
    Translation

    Target ORF information:

    RefSeq Version XM_024998807.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCTTGCTT GCCTTTTGTT
    TTAGCAGCTG CAGTATCTCG GAAGAAAAAG CGAAGAATGG GAACCTATAG CCTGGTTCCT
    AAGAAAAAGA CCAAAGTGTT AAAGCAGAGG ACGGTGATTG AGATGTTTAA GAGCATAACT
    CATTCCACGG GGGGTTCCAA GGGTGAGAAG GATCTGAGCG ATGGCTCCCT CCATGTGAAC
    GGTGAGAGCC TGGATCTGGA CTCGGAGGAG GATGACTCGG AGGAGCTGGA CGAGGATGAG
    GACCAGGGCG GCCCACCAGC AGCTGCGTTC CCTACTGAGG ATGGCAGGGC CTCCAAGGAT
    GGCGTGTTGG CGCCTGACTG CAGCCAGAAG GTGGACGGCG AGTCTGAAGA GGAGCAGGAG
    TCGGCCGGCA CCGCGGACGA GGAGGAGGAC GGAGACGAGT CCGACTTGAG TTCTGAGTCC
    AGCGTCAAGA AGAAGTTCCT CAAGAGGAAG GGGAAGCCTG ACAGCCCTTG GATCAAGCCA
    GCGAGGAAGA GGAGGCGGAG AAGCAAAAAG AAGCCCTGCT CTGTGTTAGG TTCTGAAGCC
    TCTGCGTCGT CTTCGGGCAG TGTGGAGCCA ACGGCGCCTG GCGACGGCGG GGGGTACATG
    GAGGTGCCGC TGGACTCCTT GAATCTCCAT GTCCAGGGTG CGCTGACCTC TGACACAGAA
    GGGCTGGCCA ACGGTCCTGA TGCAGGCGAG ACGGATGGCC TCCAGGAGGT GCCCCTCTGC
    AGCTGCCGGA TGGAGACCCC AAAGAGCCGG GAGATCACCA CTCTGGCCAA CAACCAGTGC
    ATGGCCACAG AGAGTGTGGA CCACCAGCTG GGCAGGTGCA CGAACAGCGT GGTGAAGCAT
    GAGCTGATGC GTCCGTCCAG CAAGGCACCG CTGCTGGTGC TGTGTGAGGA CCACCGGGGC
    CGGATGGTGA AGCACCAGTG CTGCCCCGGC TGCGGCTACT TCTGCACGGC CGGTAATTTT
    ATGGAATGTC AGCCTGAGAG CAGCATTTCT CATCGTTTCC ACAAAGCCTG TGCCTCTCGA
    GTCAATAATG CCAGCTACTG TCCCCACTGT GGGGAGGAGA CCTCCAAGGC CAAAGAGGTG
    ACCATTGCAA AGGCAGACAC GACGTCCACG GTGACCCTGG CCCCTGGGCA GGAGAAGAGC
    TGTGCAGCAG AGGGCAGGGC CGACACCACC ACGGGCAGCG CTGCTGGCCC CCTGCTCTCA
    GAGGAGGACA AGCCGCAGAG CCTGGCCCCC CAGGTGCCGG AGGGCTTCGA CCCCACAGGG
    CCGGCTGGGC TGGGGAAGCC GGCGCCAGGC CTCTCCCAGG GCCCTGGGAA GGAGACCCTG
    GAGAGCGCAC TCATCGCCCT GGATTCCGAG AAACCCAAGA AGCTCCGCTT CCACCCGAAG
    CAGCTGTACT TCTCGGCGCG GCAAGGGGAG CTGCAGAAGG TGCTGCTCAT GTTGGTTGAC
    GGGATCGACC CCAACTTCAA GATGGAGCAT CAGAACAAGC GCTCGCCCCT GCACGCCGCG
    GCGGAGGCTG GCCACGTGGA CATCTGCCAC ATGCTGATCC AGGCGGGCGC CAACATCGAC
    ACCTGCTCTG AGGACCAGAG GACCCCGCTG ATGGAGGCCG CGGAGAACAA CCACTTGGAC
    GCGGTGAAGT ACCTCATCAA GGCCGGGGCC CTCGTGGATC CCAAGGACGC AGAGGGCTCG
    ACCTGTTTGC ACCTGGCTGC CAAGAAAGGC CACTACGACG TGGTTCAGTA CTTGCTCTCA
    AACGGACAGA TGGACGTCAA CTGTCAGGAT GATGGTGGCT GGACGCCCAT GATCTGGGCC
    ACTGAGTACA AGCACGTGGA CCTCGTGAAG CTGTTGCTCT CCAAGGGGTC TGACATCAAC
    ATCCGGGACA ACGAGGAAAA CATCTGCCTG CACTGGGCGG CCTTCTCAGG CTGTGTGGAC
    ATCGCTGAGA TCCTGCTGGC CGCCAGGTGC GACCTGCACG CGGTGAACAT CCACGGGGAC
    TCGCCCCTGC ACATCGCGGC CCGGGAGGAC CGCTACGCCT GTGTCGTCCT CTTCCTGTCC
    CGGGACTCGG ATGTCACTCT GAAGAACAAG GAGGGGGAGA CTCCCCTGCA GTGCGCCAGC
    CTCAACTCAC AGGTGTGGAG CGCCCTGCAG GTGAGCCAGG CGCTGCGGGA CGCGGCCCCT
    GACAGGCCCA CCCCGGTGGA GAAGACGGTG AGCAGGGACA TTGCTCGGGG CTACGAGCGG
    ATCCCCATCC CCTGCGTCAA TGGTGTGGAC GGCGAGCCGT GCCCCAGCAA CTACAAGTAC
    GTGTCTCAGA ACTGTGTGAC GTCCCCCATG AACATCGATA GGAACATCAC CCACCTGCAG
    TACTGCGTGT GCATCGACGA CTGCTCCTCC AGCAACTGCA TGTGTGGCCA GCTCAGCATG
    CGCTGCTGGT ACGACAAGGA CGGTCGGCTG CTGCCAGAGT TCAACATGGC AGAGCCGCCC
    CTGCTGTTCG AGTGCAACCA CGCCTGCTCC TGCTGGAGGA CGTGCCGCAA CCGCGTGGTG
    CAGAATGGCC TCAGGGCGAG GCTGCAGCTC TATCGGACAC AGAACATGGG CTGGGGCGTC
    CGGTCCCTGC AGGACATCCC ACTGGGCACC TTTGTTTGCG AGTACGTTGG GGAGCTTATT
    TCTGACTCGG AAGCTGATGT GCGGGAAGAA GACTCTTATC TCTTTGATCT TGACAACAAG
    GACGGAGAGC TGTACTGCAT CGACGCGCGT TTCTATGGCA ACGTCAGCCG CTTCATCAAC
    CACCACTGTG AGCCCAATCT GGTGCCCGTG CGTGTGTTCA TGTCCCACCA GGACCTGCGC
    TTCCCGCGGA TCGCCTTCTT CAGCACCCGC CTGATTGAGG CCGGGGAGCA GCTGGGCTTC
    GACTATGGGC AGCGCTTCTG GGACATCAAG GGCAAGCTGT TCAGCTGCCG CTGCGGCTCC
    CCCAAGTGCC GGCACTCCGG GGCCGCGCTG GCCCAGCGGC AGGCCGGTGC CCAGGAGGAC
    GGGCTGCCCG ACACCAGCTC CTCGCCTGCC GACCCGCTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220430
    Clone ID Related Accession (Same CDS sequence) XM_024998808.1
    Accession Version XM_024998808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCTTGCTT GCCTTTTGTT
    TTAGCAGCTG CAGTATCTCG GAAGAAAAAG CGAAGAATGG GAACCTATAG CCTGGTTCCT
    AAGAAAAAGA CCAAAGTGTT AAAGCAGAGG ACGGTGATTG AGATGTTTAA GAGCATAACT
    CATTCCACGG GGGGTTCCAA GGGTGAGAAG GATCTGAGCG ATGGCTCCCT CCATGTGAAC
    GGTGAGAGCC TGGATCTGGA CTCGGAGGAG GATGACTCGG AGGAGCTGGA CGAGGATGAG
    GACCAGGGCG GCCCACCAGC AGCTGCGTTC CCTACTGAGG ATGGCAGGGC CTCCAAGGAT
    GGCGTGTTGG CGCCTGACTG CAGCCAGAAG GTGGACGGCG AGTCTGAAGA GGAGCAGGAG
    TCGGCCGGCA CCGCGGACGA GGAGGAGGAC GGAGACGAGT CCGACTTGAG TTCTGAGTCC
    AGCGTCAAGA AGAAGTTCCT CAAGAGGAAG GGGAAGCCTG ACAGCCCTTG GATCAAGCCA
    GCGAGGAAGA GGAGGCGGAG AAGCAAAAAG AAGCCCTGCT CTGTGTTAGG TTCTGAAGCC
    TCTGCGTCGT CTTCGGGCAG TGTGGAGCCA ACGGCGCCTG GCGACGGCGG GGGGTACATG
    GAGGTGCCGC TGGACTCCTT GAATCTCCAT GTCCAGGGGC TGGCCAACGG TCCTGATGCA
    GGCGAGACGG ATGGCCTCCA GGAGGTGCCC CTCTGCAGCT GCCGGATGGA GACCCCAAAG
    AGCCGGGAGA TCACCACTCT GGCCAACAAC CAGTGCATGG CCACAGAGAG TGTGGACCAC
    CAGCTGGGCA GGTGCACGAA CAGCGTGGTG AAGCATGAGC TGATGCGTCC GTCCAGCAAG
    GCACCGCTGC TGGTGCTGTG TGAGGACCAC CGGGGCCGGA TGGTGAAGCA CCAGTGCTGC
    CCCGGCTGCG GCTACTTCTG CACGGCCGGT AATTTTATGG AATGTCAGCC TGAGAGCAGC
    ATTTCTCATC GTTTCCACAA AGCCTGTGCC TCTCGAGTCA ATAATGCCAG CTACTGTCCC
    CACTGTGGGG AGGAGACCTC CAAGGCCAAA GAGGTGACCA TTGCAAAGGC AGACACGACG
    TCCACGGTGA CCCTGGCCCC TGGGCAGGAG AAGAGCTGTG CAGCAGAGGG CAGGGCCGAC
    ACCACCACGG GCAGCGCTGC TGGCCCCCTG CTCTCAGAGG AGGACAAGCC GCAGAGCCTG
    GCCCCCCAGG TGCCGGAGGG CTTCGACCCC ACAGGGCCGG CTGGGCTGGG GAAGCCGGCG
    CCAGGCCTCT CCCAGGGCCC TGGGAAGGAG ACCCTGGAGA GCGCACTCAT CGCCCTGGAT
    TCCGAGAAAC CCAAGAAGCT CCGCTTCCAC CCGAAGCAGC TGTACTTCTC GGCGCGGCAA
    GGGGAGCTGC AGAAGGTGCT GCTCATGTTG GTTGACGGGA TCGACCCCAA CTTCAAGATG
    GAGCATCAGA ACAAGCGCTC GCCCCTGCAC GCCGCGGCGG AGGCTGGCCA CGTGGACATC
    TGCCACATGC TGATCCAGGC GGGCGCCAAC ATCGACACCT GCTCTGAGGA CCAGAGGACC
    CCGCTGATGG AGGCCGCGGA GAACAACCAC TTGGACGCGG TGAAGTACCT CATCAAGGCC
    GGGGCCCTCG TGGATCCCAA GGACGCAGAG GGCTCGACCT GTTTGCACCT GGCTGCCAAG
    AAAGGCCACT ACGACGTGGT TCAGTACTTG CTCTCAAACG GACAGATGGA CGTCAACTGT
    CAGGATGATG GTGGCTGGAC GCCCATGATC TGGGCCACTG AGTACAAGCA CGTGGACCTC
    GTGAAGCTGT TGCTCTCCAA GGGGTCTGAC ATCAACATCC GGGACAACGA GGAAAACATC
    TGCCTGCACT GGGCGGCCTT CTCAGGCTGT GTGGACATCG CTGAGATCCT GCTGGCCGCC
    AGGTGCGACC TGCACGCGGT GAACATCCAC GGGGACTCGC CCCTGCACAT CGCGGCCCGG
    GAGGACCGCT ACGCCTGTGT CGTCCTCTTC CTGTCCCGGG ACTCGGATGT CACTCTGAAG
    AACAAGGAGG GGGAGACTCC CCTGCAGTGC GCCAGCCTCA ACTCACAGGT GTGGAGCGCC
    CTGCAGGTGA GCCAGGCGCT GCGGGACGCG GCCCCTGACA GGCCCACCCC GGTGGAGAAG
    ACGGTGAGCA GGGACATTGC TCGGGGCTAC GAGCGGATCC CCATCCCCTG CGTCAATGGT
    GTGGACGGCG AGCCGTGCCC CAGCAACTAC AAGTACGTGT CTCAGAACTG TGTGACGTCC
    CCCATGAACA TCGATAGGAA CATCACCCAC CTGCAGTACT GCGTGTGCAT CGACGACTGC
    TCCTCCAGCA ACTGCATGTG TGGCCAGCTC AGCATGCGCT GCTGGTACGA CAAGGACGGT
    CGGCTGCTGC CAGAGTTCAA CATGGCAGAG CCGCCCCTGC TGTTCGAGTG CAACCACGCC
    TGCTCCTGCT GGAGGACGTG CCGCAACCGC GTGGTGCAGA ATGGCCTCAG GGCGAGGCTG
    CAGCTCTATC GGACACAGAA CATGGGCTGG GGCGTCCGGT CCCTGCAGGA CATCCCACTG
    GGCACCTTTG TTTGCGAGTA CGTTGGGGAG CTTATTTCTG ACTCGGAAGC TGATGTGCGG
    GAAGAAGACT CTTATCTCTT TGATCTTGAC AACAAGGACG GAGAGCTGTA CTGCATCGAC
    GCGCGTTTCT ATGGCAACGT CAGCCGCTTC ATCAACCACC ACTGTGAGCC CAATCTGGTG
    CCCGTGCGTG TGTTCATGTC CCACCAGGAC CTGCGCTTCC CGCGGATCGC CTTCTTCAGC
    ACCCGCCTGA TTGAGGCCGG GGAGCAGCTG GGCTTCGACT ATGGGCAGCG CTTCTGGGAC
    ATCAAGGGCA AGCTGTTCAG CTGCCGCTGC GGCTCCCCCA AGTGCCGGCA CTCCGGGGCC
    GCGCTGGCCC AGCGGCAGGC CGGTGCCCAG GAGGACGGGC TGCCCGACAC CAGCTCCTCG
    CCTGCCGACC CGCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854576.1
    CDS42..3899
    Translation

    Target ORF information:

    RefSeq Version XM_024998808.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCTTGCTT GCCTTTTGTT
    TTAGCAGCTG CAGTATCTCG GAAGAAAAAG CGAAGAATGG GAACCTATAG CCTGGTTCCT
    AAGAAAAAGA CCAAAGTGTT AAAGCAGAGG ACGGTGATTG AGATGTTTAA GAGCATAACT
    CATTCCACGG GGGGTTCCAA GGGTGAGAAG GATCTGAGCG ATGGCTCCCT CCATGTGAAC
    GGTGAGAGCC TGGATCTGGA CTCGGAGGAG GATGACTCGG AGGAGCTGGA CGAGGATGAG
    GACCAGGGCG GCCCACCAGC AGCTGCGTTC CCTACTGAGG ATGGCAGGGC CTCCAAGGAT
    GGCGTGTTGG CGCCTGACTG CAGCCAGAAG GTGGACGGCG AGTCTGAAGA GGAGCAGGAG
    TCGGCCGGCA CCGCGGACGA GGAGGAGGAC GGAGACGAGT CCGACTTGAG TTCTGAGTCC
    AGCGTCAAGA AGAAGTTCCT CAAGAGGAAG GGGAAGCCTG ACAGCCCTTG GATCAAGCCA
    GCGAGGAAGA GGAGGCGGAG AAGCAAAAAG AAGCCCTGCT CTGTGTTAGG TTCTGAAGCC
    TCTGCGTCGT CTTCGGGCAG TGTGGAGCCA ACGGCGCCTG GCGACGGCGG GGGGTACATG
    GAGGTGCCGC TGGACTCCTT GAATCTCCAT GTCCAGGGGC TGGCCAACGG TCCTGATGCA
    GGCGAGACGG ATGGCCTCCA GGAGGTGCCC CTCTGCAGCT GCCGGATGGA GACCCCAAAG
    AGCCGGGAGA TCACCACTCT GGCCAACAAC CAGTGCATGG CCACAGAGAG TGTGGACCAC
    CAGCTGGGCA GGTGCACGAA CAGCGTGGTG AAGCATGAGC TGATGCGTCC GTCCAGCAAG
    GCACCGCTGC TGGTGCTGTG TGAGGACCAC CGGGGCCGGA TGGTGAAGCA CCAGTGCTGC
    CCCGGCTGCG GCTACTTCTG CACGGCCGGT AATTTTATGG AATGTCAGCC TGAGAGCAGC
    ATTTCTCATC GTTTCCACAA AGCCTGTGCC TCTCGAGTCA ATAATGCCAG CTACTGTCCC
    CACTGTGGGG AGGAGACCTC CAAGGCCAAA GAGGTGACCA TTGCAAAGGC AGACACGACG
    TCCACGGTGA CCCTGGCCCC TGGGCAGGAG AAGAGCTGTG CAGCAGAGGG CAGGGCCGAC
    ACCACCACGG GCAGCGCTGC TGGCCCCCTG CTCTCAGAGG AGGACAAGCC GCAGAGCCTG
    GCCCCCCAGG TGCCGGAGGG CTTCGACCCC ACAGGGCCGG CTGGGCTGGG GAAGCCGGCG
    CCAGGCCTCT CCCAGGGCCC TGGGAAGGAG ACCCTGGAGA GCGCACTCAT CGCCCTGGAT
    TCCGAGAAAC CCAAGAAGCT CCGCTTCCAC CCGAAGCAGC TGTACTTCTC GGCGCGGCAA
    GGGGAGCTGC AGAAGGTGCT GCTCATGTTG GTTGACGGGA TCGACCCCAA CTTCAAGATG
    GAGCATCAGA ACAAGCGCTC GCCCCTGCAC GCCGCGGCGG AGGCTGGCCA CGTGGACATC
    TGCCACATGC TGATCCAGGC GGGCGCCAAC ATCGACACCT GCTCTGAGGA CCAGAGGACC
    CCGCTGATGG AGGCCGCGGA GAACAACCAC TTGGACGCGG TGAAGTACCT CATCAAGGCC
    GGGGCCCTCG TGGATCCCAA GGACGCAGAG GGCTCGACCT GTTTGCACCT GGCTGCCAAG
    AAAGGCCACT ACGACGTGGT TCAGTACTTG CTCTCAAACG GACAGATGGA CGTCAACTGT
    CAGGATGATG GTGGCTGGAC GCCCATGATC TGGGCCACTG AGTACAAGCA CGTGGACCTC
    GTGAAGCTGT TGCTCTCCAA GGGGTCTGAC ATCAACATCC GGGACAACGA GGAAAACATC
    TGCCTGCACT GGGCGGCCTT CTCAGGCTGT GTGGACATCG CTGAGATCCT GCTGGCCGCC
    AGGTGCGACC TGCACGCGGT GAACATCCAC GGGGACTCGC CCCTGCACAT CGCGGCCCGG
    GAGGACCGCT ACGCCTGTGT CGTCCTCTTC CTGTCCCGGG ACTCGGATGT CACTCTGAAG
    AACAAGGAGG GGGAGACTCC CCTGCAGTGC GCCAGCCTCA ACTCACAGGT GTGGAGCGCC
    CTGCAGGTGA GCCAGGCGCT GCGGGACGCG GCCCCTGACA GGCCCACCCC GGTGGAGAAG
    ACGGTGAGCA GGGACATTGC TCGGGGCTAC GAGCGGATCC CCATCCCCTG CGTCAATGGT
    GTGGACGGCG AGCCGTGCCC CAGCAACTAC AAGTACGTGT CTCAGAACTG TGTGACGTCC
    CCCATGAACA TCGATAGGAA CATCACCCAC CTGCAGTACT GCGTGTGCAT CGACGACTGC
    TCCTCCAGCA ACTGCATGTG TGGCCAGCTC AGCATGCGCT GCTGGTACGA CAAGGACGGT
    CGGCTGCTGC CAGAGTTCAA CATGGCAGAG CCGCCCCTGC TGTTCGAGTG CAACCACGCC
    TGCTCCTGCT GGAGGACGTG CCGCAACCGC GTGGTGCAGA ATGGCCTCAG GGCGAGGCTG
    CAGCTCTATC GGACACAGAA CATGGGCTGG GGCGTCCGGT CCCTGCAGGA CATCCCACTG
    GGCACCTTTG TTTGCGAGTA CGTTGGGGAG CTTATTTCTG ACTCGGAAGC TGATGTGCGG
    GAAGAAGACT CTTATCTCTT TGATCTTGAC AACAAGGACG GAGAGCTGTA CTGCATCGAC
    GCGCGTTTCT ATGGCAACGT CAGCCGCTTC ATCAACCACC ACTGTGAGCC CAATCTGGTG
    CCCGTGCGTG TGTTCATGTC CCACCAGGAC CTGCGCTTCC CGCGGATCGC CTTCTTCAGC
    ACCCGCCTGA TTGAGGCCGG GGAGCAGCTG GGCTTCGACT ATGGGCAGCG CTTCTGGGAC
    ATCAAGGGCA AGCTGTTCAG CTGCCGCTGC GGCTCCCCCA AGTGCCGGCA CTCCGGGGCC
    GCGCTGGCCC AGCGGCAGGC CGGTGCCCAG GAGGACGGGC TGCCCGACAC CAGCTCCTCG
    CCTGCCGACC CGCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220431
    Clone ID Related Accession (Same CDS sequence) XM_024998809.1
    Accession Version XM_024998809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTG ATGACGGCTC AGCGGAACAG CAGGGAGGGG AGGCCCCGGT GGCTGCAGAC 
    GGGGAGACCA ACGGGTCCTG TGACCAGAGC GATGCCAGTG GCCACGCGGC TGCTGTGAAG
    CACCCTCAGG AGAGCACACG AGGCAGCCCT CAGGAAGGGG CCGGCCCGGG GTCCCGGATA
    GCGGAAAATG GGATTTTGGA CAGAGACCCC GAGGTGGGGA AGCAGAACCA CATCCGCTCT
    GATGGCTTCC CCCAGAATCC TGTCATTGGG AGCAACGGCT ACTTCCTTGG CAAGGCCCCC
    TTGTCTGAGC CACCACTGCG GGTGGCCAGC GCCCTGGCTT CCTCCCTGCC TGGCCACGCG
    GCGAAGACCC TCCCTGGCGG GGCTGGCAAA GGGAGGACTC CCGGTGCTTT TCCCCAGCCG
    CCGGCTGCTG TGCCGGCCAA GCCTGGGGAG GGCAGCAAGG ACACAGAGGA CAAGAAGGCC
    CCGGCCCCGG GCGCCGACGT CAAGGTCCAC AGGGCTCGGA AGACCATGCC GAAGTCCATC
    CCGGGCCTGC ACGCGGCGAG TAAGGACCCC AGGGAAGGCC GGGACGCCCG CGACCACGAG
    GAGCCGAAGG AGGAGCTGAG CAGGGGCGCC TCGGAGTTCG CACGCCAGCA GCTTTCACCC
    CCCTTCCCGT CCCTTCACCA GTCGCTACCT CAGAACCAAT GCTACGTGGC CACCGCGAAG
    GCCCAGACAG CTTGCTTGCC TTTTGTTTTA GCAGCTGCAG TATCTCGGAA GAAAAAGCGA
    AGAATGGGAA CCTATAGCCT GGTTCCTAAG AAAAAGACCA AAGTGTTAAA GCAGAGGACG
    GTGATTGAGA TGTTTAAGAG CATAACTCAT TCCACGGGGG GTTCCAAGGG TGAGAAGGAT
    CTGAGCGATG GCTCCCTCCA TGTGAACGGT GAGAGCCTGG ATCTGGACTC GGAGGAGGAT
    GACTCGGAGG AGCTGGACGA GGATGAGGAC CAGGGCGGCC CACCAGCAGC TGCGTTCCCT
    ACTGAGGATG GCAGGGCCTC CAAGGATGGC GTGTTGGCGC CTGACTGCAG CCAGAAGGTG
    GACGGCGAGT CTGAAGAGGA GCAGGAGTCG GCCGGCACCG CGGACGAGGA GGAGGACGGA
    GACGAGTCCG ACTTGAGTTC TGAGTCCAGC GTCAAGAAGA AGTTCCTCAA GAGGAAGGGG
    AAGCCTGACA GCCCTTGGAT CAAGCCAGCG AGGAAGAGGA GGCGGAGAAG CAAAAAGAAG
    CCCTGCTCTG TGTTAGGGCT GGCCAACGGT CCTGATGCAG GCGAGACGGA TGGCCTCCAG
    GAGGTGCCCC TCTGCAGCTG CCGGATGGAG ACCCCAAAGA GCCGGGAGAT CACCACTCTG
    GCCAACAACC AGTGCATGGC CACAGAGAGT GTGGACCACC AGCTGGGCAG GTGCACGAAC
    AGCGTGGTGA AGCATGAGCT GATGCGTCCG TCCAGCAAGG CACCGCTGCT GGTGCTGTGT
    GAGGACCACC GGGGCCGGAT GGTGAAGCAC CAGTGCTGCC CCGGCTGCGG CTACTTCTGC
    ACGGCCGGTA ATTTTATGGA ATGTCAGCCT GAGAGCAGCA TTTCTCATCG TTTCCACAAA
    GCCTGTGCCT CTCGAGTCAA TAATGCCAGC TACTGTCCCC ACTGTGGGGA GGAGACCTCC
    AAGGCCAAAG AGGTGACCAT TGCAAAGGCA GACACGACGT CCACGGTGAC CCTGGCCCCT
    GGGCAGGAGA AGAGCTGTGC AGCAGAGGGC AGGGCCGACA CCACCACGGG CAGCGCTGCT
    GGCCCCCTGC TCTCAGAGGA GGACAAGCCG CAGAGCCTGG CCCCCCAGGT GCCGGAGGGC
    TTCGACCCCA CAGGGCCGGC TGGGCTGGGG AAGCCGGCGC CAGGCCTCTC CCAGGGCCCT
    GGGAAGGAGA CCCTGGAGAG CGCACTCATC GCCCTGGATT CCGAGAAACC CAAGAAGCTC
    CGCTTCCACC CGAAGCAGCT GTACTTCTCG GCGCGGCAAG GGGAGCTGCA GAAGGTGCTG
    CTCATGTTGG TTGACGGGAT CGACCCCAAC TTCAAGATGG AGCATCAGAA CAAGCGCTCG
    CCCCTGCACG CCGCGGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GATCCAGGCG
    GGCGCCAACA TCGACACCTG CTCTGAGGAC CAGAGGACCC CGCTGATGGA GGCCGCGGAG
    AACAACCACT TGGACGCGGT GAAGTACCTC ATCAAGGCCG GGGCCCTCGT GGATCCCAAG
    GACGCAGAGG GCTCGACCTG TTTGCACCTG GCTGCCAAGA AAGGCCACTA CGACGTGGTT
    CAGTACTTGC TCTCAAACGG ACAGATGGAC GTCAACTGTC AGGATGATGG TGGCTGGACG
    CCCATGATCT GGGCCACTGA GTACAAGCAC GTGGACCTCG TGAAGCTGTT GCTCTCCAAG
    GGGTCTGACA TCAACATCCG GGACAACGAG GAAAACATCT GCCTGCACTG GGCGGCCTTC
    TCAGGCTGTG TGGACATCGC TGAGATCCTG CTGGCCGCCA GGTGCGACCT GCACGCGGTG
    AACATCCACG GGGACTCGCC CCTGCACATC GCGGCCCGGG AGGACCGCTA CGCCTGTGTC
    GTCCTCTTCC TGTCCCGGGA CTCGGATGTC ACTCTGAAGA ACAAGGAGGG GGAGACTCCC
    CTGCAGTGCG CCAGCCTCAA CTCACAGGTG TGGAGCGCCC TGCAGGTGAG CCAGGCGCTG
    CGGGACGCGG CCCCTGACAG GCCCACCCCG GTGGAGAAGA CGGTGAGCAG GGACATTGCT
    CGGGGCTACG AGCGGATCCC CATCCCCTGC GTCAATGGTG TGGACGGCGA GCCGTGCCCC
    AGCAACTACA AGTACGTGTC TCAGAACTGT GTGACGTCCC CCATGAACAT CGATAGGAAC
    ATCACCCACC TGCAGTACTG CGTGTGCATC GACGACTGCT CCTCCAGCAA CTGCATGTGT
    GGCCAGCTCA GCATGCGCTG CTGGTACGAC AAGGACGGTC GGCTGCTGCC AGAGTTCAAC
    ATGGCAGAGC CGCCCCTGCT GTTCGAGTGC AACCACGCCT GCTCCTGCTG GAGGACGTGC
    CGCAACCGCG TGGTGCAGAA TGGCCTCAGG GCGAGGCTGC AGCTCTATCG GACACAGAAC
    ATGGGCTGGG GCGTCCGGTC CCTGCAGGAC ATCCCACTGG GCACCTTTGT TTGCGAGTAC
    GTTGGGGAGC TTATTTCTGA CTCGGAAGCT GATGTGCGGG AAGAAGACTC TTATCTCTTT
    GATCTTGACA ACAAGGACGG AGAGCTGTAC TGCATCGACG CGCGTTTCTA TGGCAACGTC
    AGCCGCTTCA TCAACCACCA CTGTGAGCCC AATCTGGTGC CCGTGCGTGT GTTCATGTCC
    CACCAGGACC TGCGCTTCCC GCGGATCGCC TTCTTCAGCA CCCGCCTGAT TGAGGCCGGG
    GAGCAGCTGG GCTTCGACTA TGGGCAGCGC TTCTGGGACA TCAAGGGCAA GCTGTTCAGC
    TGCCGCTGCG GCTCCCCCAA GTGCCGGCAC TCCGGGGCCG CGCTGGCCCA GCGGCAGGCC
    GGTGCCCAGG AGGACGGGCT GCCCGACACC AGCTCCTCGC CTGCCGACCC GCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854577.1
    CDS73..3729
    Translation

    Target ORF information:

    RefSeq Version XM_024998809.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTG ATGACGGCTC AGCGGAACAG CAGGGAGGGG AGGCCCCGGT GGCTGCAGAC 
    GGGGAGACCA ACGGGTCCTG TGACCAGAGC GATGCCAGTG GCCACGCGGC TGCTGTGAAG
    CACCCTCAGG AGAGCACACG AGGCAGCCCT CAGGAAGGGG CCGGCCCGGG GTCCCGGATA
    GCGGAAAATG GGATTTTGGA CAGAGACCCC GAGGTGGGGA AGCAGAACCA CATCCGCTCT
    GATGGCTTCC CCCAGAATCC TGTCATTGGG AGCAACGGCT ACTTCCTTGG CAAGGCCCCC
    TTGTCTGAGC CACCACTGCG GGTGGCCAGC GCCCTGGCTT CCTCCCTGCC TGGCCACGCG
    GCGAAGACCC TCCCTGGCGG GGCTGGCAAA GGGAGGACTC CCGGTGCTTT TCCCCAGCCG
    CCGGCTGCTG TGCCGGCCAA GCCTGGGGAG GGCAGCAAGG ACACAGAGGA CAAGAAGGCC
    CCGGCCCCGG GCGCCGACGT CAAGGTCCAC AGGGCTCGGA AGACCATGCC GAAGTCCATC
    CCGGGCCTGC ACGCGGCGAG TAAGGACCCC AGGGAAGGCC GGGACGCCCG CGACCACGAG
    GAGCCGAAGG AGGAGCTGAG CAGGGGCGCC TCGGAGTTCG CACGCCAGCA GCTTTCACCC
    CCCTTCCCGT CCCTTCACCA GTCGCTACCT CAGAACCAAT GCTACGTGGC CACCGCGAAG
    GCCCAGACAG CTTGCTTGCC TTTTGTTTTA GCAGCTGCAG TATCTCGGAA GAAAAAGCGA
    AGAATGGGAA CCTATAGCCT GGTTCCTAAG AAAAAGACCA AAGTGTTAAA GCAGAGGACG
    GTGATTGAGA TGTTTAAGAG CATAACTCAT TCCACGGGGG GTTCCAAGGG TGAGAAGGAT
    CTGAGCGATG GCTCCCTCCA TGTGAACGGT GAGAGCCTGG ATCTGGACTC GGAGGAGGAT
    GACTCGGAGG AGCTGGACGA GGATGAGGAC CAGGGCGGCC CACCAGCAGC TGCGTTCCCT
    ACTGAGGATG GCAGGGCCTC CAAGGATGGC GTGTTGGCGC CTGACTGCAG CCAGAAGGTG
    GACGGCGAGT CTGAAGAGGA GCAGGAGTCG GCCGGCACCG CGGACGAGGA GGAGGACGGA
    GACGAGTCCG ACTTGAGTTC TGAGTCCAGC GTCAAGAAGA AGTTCCTCAA GAGGAAGGGG
    AAGCCTGACA GCCCTTGGAT CAAGCCAGCG AGGAAGAGGA GGCGGAGAAG CAAAAAGAAG
    CCCTGCTCTG TGTTAGGGCT GGCCAACGGT CCTGATGCAG GCGAGACGGA TGGCCTCCAG
    GAGGTGCCCC TCTGCAGCTG CCGGATGGAG ACCCCAAAGA GCCGGGAGAT CACCACTCTG
    GCCAACAACC AGTGCATGGC CACAGAGAGT GTGGACCACC AGCTGGGCAG GTGCACGAAC
    AGCGTGGTGA AGCATGAGCT GATGCGTCCG TCCAGCAAGG CACCGCTGCT GGTGCTGTGT
    GAGGACCACC GGGGCCGGAT GGTGAAGCAC CAGTGCTGCC CCGGCTGCGG CTACTTCTGC
    ACGGCCGGTA ATTTTATGGA ATGTCAGCCT GAGAGCAGCA TTTCTCATCG TTTCCACAAA
    GCCTGTGCCT CTCGAGTCAA TAATGCCAGC TACTGTCCCC ACTGTGGGGA GGAGACCTCC
    AAGGCCAAAG AGGTGACCAT TGCAAAGGCA GACACGACGT CCACGGTGAC CCTGGCCCCT
    GGGCAGGAGA AGAGCTGTGC AGCAGAGGGC AGGGCCGACA CCACCACGGG CAGCGCTGCT
    GGCCCCCTGC TCTCAGAGGA GGACAAGCCG CAGAGCCTGG CCCCCCAGGT GCCGGAGGGC
    TTCGACCCCA CAGGGCCGGC TGGGCTGGGG AAGCCGGCGC CAGGCCTCTC CCAGGGCCCT
    GGGAAGGAGA CCCTGGAGAG CGCACTCATC GCCCTGGATT CCGAGAAACC CAAGAAGCTC
    CGCTTCCACC CGAAGCAGCT GTACTTCTCG GCGCGGCAAG GGGAGCTGCA GAAGGTGCTG
    CTCATGTTGG TTGACGGGAT CGACCCCAAC TTCAAGATGG AGCATCAGAA CAAGCGCTCG
    CCCCTGCACG CCGCGGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GATCCAGGCG
    GGCGCCAACA TCGACACCTG CTCTGAGGAC CAGAGGACCC CGCTGATGGA GGCCGCGGAG
    AACAACCACT TGGACGCGGT GAAGTACCTC ATCAAGGCCG GGGCCCTCGT GGATCCCAAG
    GACGCAGAGG GCTCGACCTG TTTGCACCTG GCTGCCAAGA AAGGCCACTA CGACGTGGTT
    CAGTACTTGC TCTCAAACGG ACAGATGGAC GTCAACTGTC AGGATGATGG TGGCTGGACG
    CCCATGATCT GGGCCACTGA GTACAAGCAC GTGGACCTCG TGAAGCTGTT GCTCTCCAAG
    GGGTCTGACA TCAACATCCG GGACAACGAG GAAAACATCT GCCTGCACTG GGCGGCCTTC
    TCAGGCTGTG TGGACATCGC TGAGATCCTG CTGGCCGCCA GGTGCGACCT GCACGCGGTG
    AACATCCACG GGGACTCGCC CCTGCACATC GCGGCCCGGG AGGACCGCTA CGCCTGTGTC
    GTCCTCTTCC TGTCCCGGGA CTCGGATGTC ACTCTGAAGA ACAAGGAGGG GGAGACTCCC
    CTGCAGTGCG CCAGCCTCAA CTCACAGGTG TGGAGCGCCC TGCAGGTGAG CCAGGCGCTG
    CGGGACGCGG CCCCTGACAG GCCCACCCCG GTGGAGAAGA CGGTGAGCAG GGACATTGCT
    CGGGGCTACG AGCGGATCCC CATCCCCTGC GTCAATGGTG TGGACGGCGA GCCGTGCCCC
    AGCAACTACA AGTACGTGTC TCAGAACTGT GTGACGTCCC CCATGAACAT CGATAGGAAC
    ATCACCCACC TGCAGTACTG CGTGTGCATC GACGACTGCT CCTCCAGCAA CTGCATGTGT
    GGCCAGCTCA GCATGCGCTG CTGGTACGAC AAGGACGGTC GGCTGCTGCC AGAGTTCAAC
    ATGGCAGAGC CGCCCCTGCT GTTCGAGTGC AACCACGCCT GCTCCTGCTG GAGGACGTGC
    CGCAACCGCG TGGTGCAGAA TGGCCTCAGG GCGAGGCTGC AGCTCTATCG GACACAGAAC
    ATGGGCTGGG GCGTCCGGTC CCTGCAGGAC ATCCCACTGG GCACCTTTGT TTGCGAGTAC
    GTTGGGGAGC TTATTTCTGA CTCGGAAGCT GATGTGCGGG AAGAAGACTC TTATCTCTTT
    GATCTTGACA ACAAGGACGG AGAGCTGTAC TGCATCGACG CGCGTTTCTA TGGCAACGTC
    AGCCGCTTCA TCAACCACCA CTGTGAGCCC AATCTGGTGC CCGTGCGTGT GTTCATGTCC
    CACCAGGACC TGCGCTTCCC GCGGATCGCC TTCTTCAGCA CCCGCCTGAT TGAGGCCGGG
    GAGCAGCTGG GCTTCGACTA TGGGCAGCGC TTCTGGGACA TCAAGGGCAA GCTGTTCAGC
    TGCCGCTGCG GCTCCCCCAA GTGCCGGCAC TCCGGGGCCG CGCTGGCCCA GCGGCAGGCC
    GGTGCCCAGG AGGACGGGCT GCCCGACACC AGCTCCTCGC CTGCCGACCC GCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220432
    Clone ID Related Accession (Same CDS sequence) XM_024998810.1
    Accession Version XM_024998810.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCAGCTGC AGTATCTCGG
    AAGAAAAAGC GAAGAATGGG AACCTATAGC CTGGTTCCTA AGAAAAAGAC CAAAGTGTTA
    AAGCAGAGGA CGGTGATTGA GATGTTTAAG AGCATAACTC ATTCCACGGG GGGTTCCAAG
    GGTGAGAAGG ATCTGAGCGA TGGCTCCCTC CATGTGAACG GTGAGAGCCT GGATCTGGAC
    TCGGAGGAGG ATGACTCGGA GGAGCTGGAC GAGGATGAGG ACCAGGGCGG CCCACCAGCA
    GCTGCGTTCC CTACTGAGGA TGGCAGGGCC TCCAAGGATG GCGTGTTGGC GCCTGACTGC
    AGCCAGAAGG TGGACGGCGA GTCTGAAGAG GAGCAGGAGT CGGCCGGCAC CGCGGACGAG
    GAGGAGGACG GAGACGAGTC CGACTTGAGT TCTGAGTCCA GCGTCAAGAA GAAGTTCCTC
    AAGAGGAAGG GGAAGCCTGA CAGCCCTTGG ATCAAGCCAG CGAGGAAGAG GAGGCGGAGA
    AGCAAAAAGA AGCCCTGCTC TGTGTTAGGT TCTGAAGCCT CTGCGTCGTC TTCGGGCAGT
    GTGGAGCCAA CGGCGCCTGG CGACGGCGGG GGGTACATGG AGGTGCCGCT GGACTCCTTG
    AATCTCCATG TCCAGGGGCT GGCCAACGGT CCTGATGCAG GCGAGACGGA TGGCCTCCAG
    GAGGTGCCCC TCTGCAGCTG CCGGATGGAG ACCCCAAAGA GCCGGGAGAT CACCACTCTG
    GCCAACAACC AGTGCATGGC CACAGAGAGT GTGGACCACC AGCTGGGCAG GTGCACGAAC
    AGCGTGGTGA AGCATGAGCT GATGCGTCCG TCCAGCAAGG CACCGCTGCT GGTGCTGTGT
    GAGGACCACC GGGGCCGGAT GGTGAAGCAC CAGTGCTGCC CCGGCTGCGG CTACTTCTGC
    ACGGCCGGTA ATTTTATGGA ATGTCAGCCT GAGAGCAGCA TTTCTCATCG TTTCCACAAA
    GCCTGTGCCT CTCGAGTCAA TAATGCCAGC TACTGTCCCC ACTGTGGGGA GGAGACCTCC
    AAGGCCAAAG AGGTGACCAT TGCAAAGGCA GACACGACGT CCACGGTGAC CCTGGCCCCT
    GGGCAGGAGA AGAGCTGTGC AGCAGAGGGC AGGGCCGACA CCACCACGGG CAGCGCTGCT
    GGCCCCCTGC TCTCAGAGGA GGACAAGCCG CAGAGCCTGG CCCCCCAGGT GCCGGAGGGC
    TTCGACCCCA CAGGGCCGGC TGGGCTGGGG AAGCCGGCGC CAGGCCTCTC CCAGGGCCCT
    GGGAAGGAGA CCCTGGAGAG CGCACTCATC GCCCTGGATT CCGAGAAACC CAAGAAGCTC
    CGCTTCCACC CGAAGCAGCT GTACTTCTCG GCGCGGCAAG GGGAGCTGCA GAAGGTGCTG
    CTCATGTTGG TTGACGGGAT CGACCCCAAC TTCAAGATGG AGCATCAGAA CAAGCGCTCG
    CCCCTGCACG CCGCGGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GATCCAGGCG
    GGCGCCAACA TCGACACCTG CTCTGAGGAC CAGAGGACCC CGCTGATGGA GGCCGCGGAG
    AACAACCACT TGGACGCGGT GAAGTACCTC ATCAAGGCCG GGGCCCTCGT GGATCCCAAG
    GACGCAGAGG GCTCGACCTG TTTGCACCTG GCTGCCAAGA AAGGCCACTA CGACGTGGTT
    CAGTACTTGC TCTCAAACGG ACAGATGGAC GTCAACTGTC AGGATGATGG TGGCTGGACG
    CCCATGATCT GGGCCACTGA GTACAAGCAC GTGGACCTCG TGAAGCTGTT GCTCTCCAAG
    GGGTCTGACA TCAACATCCG GGACAACGAG GAAAACATCT GCCTGCACTG GGCGGCCTTC
    TCAGGCTGTG TGGACATCGC TGAGATCCTG CTGGCCGCCA GGTGCGACCT GCACGCGGTG
    AACATCCACG GGGACTCGCC CCTGCACATC GCGGCCCGGG AGGACCGCTA CGCCTGTGTC
    GTCCTCTTCC TGTCCCGGGA CTCGGATGTC ACTCTGAAGA ACAAGGAGGG GGAGACTCCC
    CTGCAGTGCG CCAGCCTCAA CTCACAGGTG TGGAGCGCCC TGCAGGTGAG CCAGGCGCTG
    CGGGACGCGG CCCCTGACAG GCCCACCCCG GTGGAGAAGA CGGTGAGCAG GGACATTGCT
    CGGGGCTACG AGCGGATCCC CATCCCCTGC GTCAATGGTG TGGACGGCGA GCCGTGCCCC
    AGCAACTACA AGTACGTGTC TCAGAACTGT GTGACGTCCC CCATGAACAT CGATAGGAAC
    ATCACCCACC TGCAGTACTG CGTGTGCATC GACGACTGCT CCTCCAGCAA CTGCATGTGT
    GGCCAGCTCA GCATGCGCTG CTGGTACGAC AAGGACGGTC GGCTGCTGCC AGAGTTCAAC
    ATGGCAGAGC CGCCCCTGCT GTTCGAGTGC AACCACGCCT GCTCCTGCTG GAGGACGTGC
    CGCAACCGCG TGGTGCAGAA TGGCCTCAGG GCGAGGCTGC AGCTCTATCG GACACAGAAC
    ATGGGCTGGG GCGTCCGGTC CCTGCAGGAC ATCCCACTGG GCACCTTTGT TTGCGAGTAC
    GTTGGGGAGC TTATTTCTGA CTCGGAAGCT GATGTGCGGG AAGAAGACTC TTATCTCTTT
    GATCTTGACA ACAAGGACGG AGAGCTGTAC TGCATCGACG CGCGTTTCTA TGGCAACGTC
    AGCCGCTTCA TCAACCACCA CTGTGAGCCC AATCTGGTGC CCGTGCGTGT GTTCATGTCC
    CACCAGGACC TGCGCTTCCC GCGGATCGCC TTCTTCAGCA CCCGCCTGAT TGAGGCCGGG
    GAGCAGCTGG GCTTCGACTA TGGGCAGCGC TTCTGGGACA TCAAGGGCAA GCTGTTCAGC
    TGCCGCTGCG GCTCCCCCAA GTGCCGGCAC TCCGGGGCCG CGCTGGCCCA GCGGCAGGCC
    GGTGCCCAGG AGGACGGGCT GCCCGACACC AGCTCCTCGC CTGCCGACCC GCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854578.1
    CDS42..3878
    Translation

    Target ORF information:

    RefSeq Version XM_024998810.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998810.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCAGCTGC AGTATCTCGG
    AAGAAAAAGC GAAGAATGGG AACCTATAGC CTGGTTCCTA AGAAAAAGAC CAAAGTGTTA
    AAGCAGAGGA CGGTGATTGA GATGTTTAAG AGCATAACTC ATTCCACGGG GGGTTCCAAG
    GGTGAGAAGG ATCTGAGCGA TGGCTCCCTC CATGTGAACG GTGAGAGCCT GGATCTGGAC
    TCGGAGGAGG ATGACTCGGA GGAGCTGGAC GAGGATGAGG ACCAGGGCGG CCCACCAGCA
    GCTGCGTTCC CTACTGAGGA TGGCAGGGCC TCCAAGGATG GCGTGTTGGC GCCTGACTGC
    AGCCAGAAGG TGGACGGCGA GTCTGAAGAG GAGCAGGAGT CGGCCGGCAC CGCGGACGAG
    GAGGAGGACG GAGACGAGTC CGACTTGAGT TCTGAGTCCA GCGTCAAGAA GAAGTTCCTC
    AAGAGGAAGG GGAAGCCTGA CAGCCCTTGG ATCAAGCCAG CGAGGAAGAG GAGGCGGAGA
    AGCAAAAAGA AGCCCTGCTC TGTGTTAGGT TCTGAAGCCT CTGCGTCGTC TTCGGGCAGT
    GTGGAGCCAA CGGCGCCTGG CGACGGCGGG GGGTACATGG AGGTGCCGCT GGACTCCTTG
    AATCTCCATG TCCAGGGGCT GGCCAACGGT CCTGATGCAG GCGAGACGGA TGGCCTCCAG
    GAGGTGCCCC TCTGCAGCTG CCGGATGGAG ACCCCAAAGA GCCGGGAGAT CACCACTCTG
    GCCAACAACC AGTGCATGGC CACAGAGAGT GTGGACCACC AGCTGGGCAG GTGCACGAAC
    AGCGTGGTGA AGCATGAGCT GATGCGTCCG TCCAGCAAGG CACCGCTGCT GGTGCTGTGT
    GAGGACCACC GGGGCCGGAT GGTGAAGCAC CAGTGCTGCC CCGGCTGCGG CTACTTCTGC
    ACGGCCGGTA ATTTTATGGA ATGTCAGCCT GAGAGCAGCA TTTCTCATCG TTTCCACAAA
    GCCTGTGCCT CTCGAGTCAA TAATGCCAGC TACTGTCCCC ACTGTGGGGA GGAGACCTCC
    AAGGCCAAAG AGGTGACCAT TGCAAAGGCA GACACGACGT CCACGGTGAC CCTGGCCCCT
    GGGCAGGAGA AGAGCTGTGC AGCAGAGGGC AGGGCCGACA CCACCACGGG CAGCGCTGCT
    GGCCCCCTGC TCTCAGAGGA GGACAAGCCG CAGAGCCTGG CCCCCCAGGT GCCGGAGGGC
    TTCGACCCCA CAGGGCCGGC TGGGCTGGGG AAGCCGGCGC CAGGCCTCTC CCAGGGCCCT
    GGGAAGGAGA CCCTGGAGAG CGCACTCATC GCCCTGGATT CCGAGAAACC CAAGAAGCTC
    CGCTTCCACC CGAAGCAGCT GTACTTCTCG GCGCGGCAAG GGGAGCTGCA GAAGGTGCTG
    CTCATGTTGG TTGACGGGAT CGACCCCAAC TTCAAGATGG AGCATCAGAA CAAGCGCTCG
    CCCCTGCACG CCGCGGCGGA GGCTGGCCAC GTGGACATCT GCCACATGCT GATCCAGGCG
    GGCGCCAACA TCGACACCTG CTCTGAGGAC CAGAGGACCC CGCTGATGGA GGCCGCGGAG
    AACAACCACT TGGACGCGGT GAAGTACCTC ATCAAGGCCG GGGCCCTCGT GGATCCCAAG
    GACGCAGAGG GCTCGACCTG TTTGCACCTG GCTGCCAAGA AAGGCCACTA CGACGTGGTT
    CAGTACTTGC TCTCAAACGG ACAGATGGAC GTCAACTGTC AGGATGATGG TGGCTGGACG
    CCCATGATCT GGGCCACTGA GTACAAGCAC GTGGACCTCG TGAAGCTGTT GCTCTCCAAG
    GGGTCTGACA TCAACATCCG GGACAACGAG GAAAACATCT GCCTGCACTG GGCGGCCTTC
    TCAGGCTGTG TGGACATCGC TGAGATCCTG CTGGCCGCCA GGTGCGACCT GCACGCGGTG
    AACATCCACG GGGACTCGCC CCTGCACATC GCGGCCCGGG AGGACCGCTA CGCCTGTGTC
    GTCCTCTTCC TGTCCCGGGA CTCGGATGTC ACTCTGAAGA ACAAGGAGGG GGAGACTCCC
    CTGCAGTGCG CCAGCCTCAA CTCACAGGTG TGGAGCGCCC TGCAGGTGAG CCAGGCGCTG
    CGGGACGCGG CCCCTGACAG GCCCACCCCG GTGGAGAAGA CGGTGAGCAG GGACATTGCT
    CGGGGCTACG AGCGGATCCC CATCCCCTGC GTCAATGGTG TGGACGGCGA GCCGTGCCCC
    AGCAACTACA AGTACGTGTC TCAGAACTGT GTGACGTCCC CCATGAACAT CGATAGGAAC
    ATCACCCACC TGCAGTACTG CGTGTGCATC GACGACTGCT CCTCCAGCAA CTGCATGTGT
    GGCCAGCTCA GCATGCGCTG CTGGTACGAC AAGGACGGTC GGCTGCTGCC AGAGTTCAAC
    ATGGCAGAGC CGCCCCTGCT GTTCGAGTGC AACCACGCCT GCTCCTGCTG GAGGACGTGC
    CGCAACCGCG TGGTGCAGAA TGGCCTCAGG GCGAGGCTGC AGCTCTATCG GACACAGAAC
    ATGGGCTGGG GCGTCCGGTC CCTGCAGGAC ATCCCACTGG GCACCTTTGT TTGCGAGTAC
    GTTGGGGAGC TTATTTCTGA CTCGGAAGCT GATGTGCGGG AAGAAGACTC TTATCTCTTT
    GATCTTGACA ACAAGGACGG AGAGCTGTAC TGCATCGACG CGCGTTTCTA TGGCAACGTC
    AGCCGCTTCA TCAACCACCA CTGTGAGCCC AATCTGGTGC CCGTGCGTGT GTTCATGTCC
    CACCAGGACC TGCGCTTCCC GCGGATCGCC TTCTTCAGCA CCCGCCTGAT TGAGGCCGGG
    GAGCAGCTGG GCTTCGACTA TGGGCAGCGC TTCTGGGACA TCAAGGGCAA GCTGTTCAGC
    TGCCGCTGCG GCTCCCCCAA GTGCCGGCAC TCCGGGGCCG CGCTGGCCCA GCGGCAGGCC
    GGTGCCCAGG AGGACGGGCT GCCCGACACC AGCTCCTCGC CTGCCGACCC GCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220433
    Clone ID Related Accession (Same CDS sequence) XM_024998811.1
    Accession Version XM_024998811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTGCTG ATGACGGCTC AGCGGAACAG CAGGGAGGGG AGGCCCCGGT GGCTGCAGAC 
    GGGGAGACCA ACGGGTCCTG TGACCAGAGC GATGCCAGTG GCCACGCGGC TGCTGTGAAG
    CACCCTCAGG AGAGCACACG AGGCAGCCCT CAGGAAGGGG CCGGCCCGGG GTCCCGGATA
    GCGGAAAATG GGATTTTGGA CAGAGACCCC GAGGTGGGGA AGCAGAACCA CATCCGCTCT
    GATGGCTTCC CCCAGAATCC TGTCATTGGG AGCAACGGCT ACTTCCTTGG CAAGGCCCCC
    TTGTCTGAGC CACCACTGCG GGTGGCCAGC GCCCTGGCTT CCTCCCTGCC TGGCCACGCG
    GCGAAGACCC TCCCTGGCGG GGCTGGCAAA GGGAGGACTC CCGGTGCTTT TCCCCAGCCG
    CCGGCTGCTG TGCCGGCCAA GCCTGGGGAG GGCAGCAAGG ACACAGAGGA CAAGAAGGCC
    CCGGCCCCGG GCGCCGACGT CAAGGTCCAC AGGGCTCGGA AGACCATGCC GAAGTCCATC
    CCGGGCCTGC ACGCGGCGAG TAAGGACCCC AGGGAAGGCC GGGACGCCCG CGACCACGAG
    GAGCCGAAGG AGGAGCTGAG CAGGGGCGCC TCGGAGTTCG CACGCCAGCA GCTTTCACCC
    CCCTTCCCGT CCCTTCACCA GTCGCTACCT CAGAACCAAT GCTACGTGGC CACCGCGAAG
    GCCCAGACAG CTTGCTTGCC TTTTGTTTTA GCAGCTGCAG TATCTCGGAA GAAAAAGCGA
    AGAATGGGAA CCTATAGCCT GGTTCCTAAG AAAAAGACCA AAGTGTTAAA GCAGAGGACG
    GTGATTGAGA TGTTTAAGAG CATAACTCAT TCCACGGGGG GTTCCAAGGG TGAGAAGGAT
    CTGAGCGATG GCTCCCTCCA TGTGAACGGT GAGAGCCTGG ATCTGGACTC GGAGGAGGAT
    GACTCGGAGG AGCTGGACGA GGATGAGGAC CAGGGCGGCC CACCAGCAGC TGCGTTCCCT
    ACTGAGGATG GCAGGGCCTC CAAGGATGGC GTGTTGGCGC CTGACTGCAG CCAGAAGGTG
    GACGGCGAGT CTGAAGAGGA GCAGGAGTCG GCCGGCACCG CGGACGAGGA GGAGGACGGA
    GACGAGTCCG ACTTGAGTTC TGAGTCCAGC GTCAAGAAGA AGTTCCTCAA GAGGAAGGGG
    AAGCCTGACA GCCCTTGGAT CAAGCCAGCG AGGAAGAGGA GGCGGAGAAG CAAAAAGAAG
    CCCTGCTCTG TGTTAGGTTC TGAAGCCTCT GCGTCGTCTT CGGGCAGTGT GGAGCCAACG
    GCGCCTGGCG ACGGCGGGGG GTACATGGAG GTGCCGCTGG ACTCCTTGAA TCTCCATGTC
    CAGGGTGCGC TGACCTCTGA CACAGAAGGG CTGGCCAACG GTCCTGATGC AGGCGAGACG
    GATGGCCTCC AGGAGGTGCC CCTCTGCAGC TGCCGGATGG AGACCCCAAA GAGCCGGGAG
    ATCACCACTC TGGCCAACAA CCAGTGCATG GCCACAGAGA GTGTGGACCA CCAGCTGGGC
    AGGTGCACGA ACAGCGTGGT GAAGCATGAG CTGATGCGTC CGTCCAGCAA GGCACCGCTG
    CTGGTGCTGT GTGAGGACCA CCGGGGCCGG ATGGTGAAGC ACCAGTGCTG CCCCGGCTGC
    GGCTACTTCT GCACGGCCGG TAATTTTATG GAATGTCAGC CTGAGAGCAG CATTTCTCAT
    CGTTTCCACA AAGCCTGTGC CTCTCGAGTC AATAATGCCA GCTACTGTCC CCACTGTGGG
    GAGGAGACCT CCAAGGCCAA AGAGGTGACC ATTGCAAAGG CAGACACGAC GTCCACGGTG
    ACCCTGGCCC CTGGGCAGGA GAAGAGCTGT GCAGCAGAGG GCAGGGCCGA CACCACCACG
    GGCAGCGCTG CTGGCCCCCT GCTCTCAGAG GAGGACAAGC CGCAGAGCCT GGCCCCCCAG
    GTGCCGGAGG GCTTCGACCC CACAGGGCCG GCTGGGCTGG GGAAGCCGGC GCCAGGCCTC
    TCCCAGGGCC CTGGGAAGGA GACCCTGGAG AGCGCACTCA TCGCCCTGGA TTCCGAGAAA
    CCCAAGAAGC TCCGCTTCCA CCCGAAGCAG CTGTACTTCT CGGCGCGGCA AGGGGAGCTG
    CAGAAGGTGC TGCTCATGTT GGTTGACGGG ATCGACCCCA ACTTCAAGAT GGAGCATCAG
    AACAAGCGCT CGCCCCTGCA CGCCGCGGCG GAGGCTGGCC ACGTGGACAT CTGCCACATG
    CTGATCCAGG CGGGCGCCAA CATCGACACC TGCTCTGAGG ACCAGAGGAC CCCGCTGATG
    GAGGCCGCGG AGAACAACCA CTTGGACGCG GTGAAGTACC TCATCAAGGC CGGGGCCCTC
    GTGGATCCCA AGGACGCAGA GGGCTCGACC TGTTTGCACC TGGCTGCCAA GAAAGGCCAC
    TACGACGTGG TTCAGTACTT GCTCTCAAAC GGACAGATGG ACGTCAACTG TCAGGATGAT
    GGTGGCTGGA CGCCCATGAT CTGGGCCACT GAGTACAAGC ACGTGGACCT CGTGAAGCTG
    TTGCTCTCCA AGGGGTCTGA CATCAACATC CGGGACAACG AGGAAAACAT CTGCCTGCAC
    TGGGCGGCCT TCTCAGGCTG TGTGGACATC GCTGAGATCC TGCTGGCCGC CAGGTGCGAC
    CTGCACGCGG TGAACATCCA CGGGGACTCG CCCCTGCACA TCGCGGCCCG GGAGGACCGC
    TACGCCTGTG TCGTCCTCTT CCTGTCCCGG GACTCGGATG TCACTCTGAA GAACAAGGAG
    GGGGAGACTC CCCTGCAGTG CGCCAGCCTC AACTCACAGG TGTGGAGCGC CCTGCAGGTG
    AGCCAGGCGC TGCGGGACGC GGCCCCTGAC AGGCCCACCC CGGTGGAGAA GACGGTGAGC
    AGGGACATTG CTCGGGGCTA CGAGCGGATC CCCATCCCCT GCGTCAATGG TGTGGACGGC
    GAGCCGTGCC CCAGCAACTA CAAGTACGTG TCTCAGAACT GTGTGACGTC CCCCATGAAC
    ATCGATAGGA ACATCACCCA CCTGCAGTAC TGCGTGTGCA TCGACGACTG CTCCTCCAGC
    AACTGCATGT GTGGCCAGCT CAGCATGCGC TGCTGGTACG ACAAGGACGG TCGGCTGCTG
    CCAGAGTTCA ACATGGCAGA GCCGCCCCTG CTGTTCGAGT GCAACCACGC CTGCTCCTGC
    TGGAGGACGT GCCGCAACCG CGTGGTGCAG AATGGCCTCA GGGCGAGGCT GCAGCTCTAT
    CGGACACAGA ACATGGGCTG GGGCGTCCGG TCCCTGCAGG ACATCCCACT GGGCACCTTT
    GTTTGCGAGT ACGTTGGGGA GCTTATTTCT GACTCGGAAG CTGATGTGCG GGAAGAAGAC
    TCTTATCTCT TTGATCTTGA CAACAAGGAC GGAGAGCTGT ACTGCATCGA CGCGCGTTTC
    TATGGCAACG TCAGCCGCTT CATCAACCAC CACTGTGAGC CCAATCTGGT GCCCGTGCGT
    GTGTTCATGT CCCACCAGGA CCTGCGCTTC CCGCGGATCG CCTTCTTCAG CACCCGCCTG
    ATTGAGGCCG GGGAGCAGCT GGGCTTCGAC TATGGGCAGC GCTTCTGGGA CATCAAGGGC
    AAGCTGTTCA GCTGCCGCTG CGGCTCCCCC AAGTGCCGGC ACTCCGGGGC CGCGCTGGCC
    CAGCGGCAGG CCGGTGCCCA GGAGGACGGG CTGCCCGACA CCAGCTCCTC GCCTGCCGAC
    CCGCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854579.1
    CDS855..4643
    Translation

    Target ORF information:

    RefSeq Version XM_024998811.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTGCTG ATGACGGCTC AGCGGAACAG CAGGGAGGGG AGGCCCCGGT GGCTGCAGAC 
    GGGGAGACCA ACGGGTCCTG TGACCAGAGC GATGCCAGTG GCCACGCGGC TGCTGTGAAG
    CACCCTCAGG AGAGCACACG AGGCAGCCCT CAGGAAGGGG CCGGCCCGGG GTCCCGGATA
    GCGGAAAATG GGATTTTGGA CAGAGACCCC GAGGTGGGGA AGCAGAACCA CATCCGCTCT
    GATGGCTTCC CCCAGAATCC TGTCATTGGG AGCAACGGCT ACTTCCTTGG CAAGGCCCCC
    TTGTCTGAGC CACCACTGCG GGTGGCCAGC GCCCTGGCTT CCTCCCTGCC TGGCCACGCG
    GCGAAGACCC TCCCTGGCGG GGCTGGCAAA GGGAGGACTC CCGGTGCTTT TCCCCAGCCG
    CCGGCTGCTG TGCCGGCCAA GCCTGGGGAG GGCAGCAAGG ACACAGAGGA CAAGAAGGCC
    CCGGCCCCGG GCGCCGACGT CAAGGTCCAC AGGGCTCGGA AGACCATGCC GAAGTCCATC
    CCGGGCCTGC ACGCGGCGAG TAAGGACCCC AGGGAAGGCC GGGACGCCCG CGACCACGAG
    GAGCCGAAGG AGGAGCTGAG CAGGGGCGCC TCGGAGTTCG CACGCCAGCA GCTTTCACCC
    CCCTTCCCGT CCCTTCACCA GTCGCTACCT CAGAACCAAT GCTACGTGGC CACCGCGAAG
    GCCCAGACAG CTTGCTTGCC TTTTGTTTTA GCAGCTGCAG TATCTCGGAA GAAAAAGCGA
    AGAATGGGAA CCTATAGCCT GGTTCCTAAG AAAAAGACCA AAGTGTTAAA GCAGAGGACG
    GTGATTGAGA TGTTTAAGAG CATAACTCAT TCCACGGGGG GTTCCAAGGG TGAGAAGGAT
    CTGAGCGATG GCTCCCTCCA TGTGAACGGT GAGAGCCTGG ATCTGGACTC GGAGGAGGAT
    GACTCGGAGG AGCTGGACGA GGATGAGGAC CAGGGCGGCC CACCAGCAGC TGCGTTCCCT
    ACTGAGGATG GCAGGGCCTC CAAGGATGGC GTGTTGGCGC CTGACTGCAG CCAGAAGGTG
    GACGGCGAGT CTGAAGAGGA GCAGGAGTCG GCCGGCACCG CGGACGAGGA GGAGGACGGA
    GACGAGTCCG ACTTGAGTTC TGAGTCCAGC GTCAAGAAGA AGTTCCTCAA GAGGAAGGGG
    AAGCCTGACA GCCCTTGGAT CAAGCCAGCG AGGAAGAGGA GGCGGAGAAG CAAAAAGAAG
    CCCTGCTCTG TGTTAGGTTC TGAAGCCTCT GCGTCGTCTT CGGGCAGTGT GGAGCCAACG
    GCGCCTGGCG ACGGCGGGGG GTACATGGAG GTGCCGCTGG ACTCCTTGAA TCTCCATGTC
    CAGGGTGCGC TGACCTCTGA CACAGAAGGG CTGGCCAACG GTCCTGATGC AGGCGAGACG
    GATGGCCTCC AGGAGGTGCC CCTCTGCAGC TGCCGGATGG AGACCCCAAA GAGCCGGGAG
    ATCACCACTC TGGCCAACAA CCAGTGCATG GCCACAGAGA GTGTGGACCA CCAGCTGGGC
    AGGTGCACGA ACAGCGTGGT GAAGCATGAG CTGATGCGTC CGTCCAGCAA GGCACCGCTG
    CTGGTGCTGT GTGAGGACCA CCGGGGCCGG ATGGTGAAGC ACCAGTGCTG CCCCGGCTGC
    GGCTACTTCT GCACGGCCGG TAATTTTATG GAATGTCAGC CTGAGAGCAG CATTTCTCAT
    CGTTTCCACA AAGCCTGTGC CTCTCGAGTC AATAATGCCA GCTACTGTCC CCACTGTGGG
    GAGGAGACCT CCAAGGCCAA AGAGGTGACC ATTGCAAAGG CAGACACGAC GTCCACGGTG
    ACCCTGGCCC CTGGGCAGGA GAAGAGCTGT GCAGCAGAGG GCAGGGCCGA CACCACCACG
    GGCAGCGCTG CTGGCCCCCT GCTCTCAGAG GAGGACAAGC CGCAGAGCCT GGCCCCCCAG
    GTGCCGGAGG GCTTCGACCC CACAGGGCCG GCTGGGCTGG GGAAGCCGGC GCCAGGCCTC
    TCCCAGGGCC CTGGGAAGGA GACCCTGGAG AGCGCACTCA TCGCCCTGGA TTCCGAGAAA
    CCCAAGAAGC TCCGCTTCCA CCCGAAGCAG CTGTACTTCT CGGCGCGGCA AGGGGAGCTG
    CAGAAGGTGC TGCTCATGTT GGTTGACGGG ATCGACCCCA ACTTCAAGAT GGAGCATCAG
    AACAAGCGCT CGCCCCTGCA CGCCGCGGCG GAGGCTGGCC ACGTGGACAT CTGCCACATG
    CTGATCCAGG CGGGCGCCAA CATCGACACC TGCTCTGAGG ACCAGAGGAC CCCGCTGATG
    GAGGCCGCGG AGAACAACCA CTTGGACGCG GTGAAGTACC TCATCAAGGC CGGGGCCCTC
    GTGGATCCCA AGGACGCAGA GGGCTCGACC TGTTTGCACC TGGCTGCCAA GAAAGGCCAC
    TACGACGTGG TTCAGTACTT GCTCTCAAAC GGACAGATGG ACGTCAACTG TCAGGATGAT
    GGTGGCTGGA CGCCCATGAT CTGGGCCACT GAGTACAAGC ACGTGGACCT CGTGAAGCTG
    TTGCTCTCCA AGGGGTCTGA CATCAACATC CGGGACAACG AGGAAAACAT CTGCCTGCAC
    TGGGCGGCCT TCTCAGGCTG TGTGGACATC GCTGAGATCC TGCTGGCCGC CAGGTGCGAC
    CTGCACGCGG TGAACATCCA CGGGGACTCG CCCCTGCACA TCGCGGCCCG GGAGGACCGC
    TACGCCTGTG TCGTCCTCTT CCTGTCCCGG GACTCGGATG TCACTCTGAA GAACAAGGAG
    GGGGAGACTC CCCTGCAGTG CGCCAGCCTC AACTCACAGG TGTGGAGCGC CCTGCAGGTG
    AGCCAGGCGC TGCGGGACGC GGCCCCTGAC AGGCCCACCC CGGTGGAGAA GACGGTGAGC
    AGGGACATTG CTCGGGGCTA CGAGCGGATC CCCATCCCCT GCGTCAATGG TGTGGACGGC
    GAGCCGTGCC CCAGCAACTA CAAGTACGTG TCTCAGAACT GTGTGACGTC CCCCATGAAC
    ATCGATAGGA ACATCACCCA CCTGCAGTAC TGCGTGTGCA TCGACGACTG CTCCTCCAGC
    AACTGCATGT GTGGCCAGCT CAGCATGCGC TGCTGGTACG ACAAGGACGG TCGGCTGCTG
    CCAGAGTTCA ACATGGCAGA GCCGCCCCTG CTGTTCGAGT GCAACCACGC CTGCTCCTGC
    TGGAGGACGT GCCGCAACCG CGTGGTGCAG AATGGCCTCA GGGCGAGGCT GCAGCTCTAT
    CGGACACAGA ACATGGGCTG GGGCGTCCGG TCCCTGCAGG ACATCCCACT GGGCACCTTT
    GTTTGCGAGT ACGTTGGGGA GCTTATTTCT GACTCGGAAG CTGATGTGCG GGAAGAAGAC
    TCTTATCTCT TTGATCTTGA CAACAAGGAC GGAGAGCTGT ACTGCATCGA CGCGCGTTTC
    TATGGCAACG TCAGCCGCTT CATCAACCAC CACTGTGAGC CCAATCTGGT GCCCGTGCGT
    GTGTTCATGT CCCACCAGGA CCTGCGCTTC CCGCGGATCG CCTTCTTCAG CACCCGCCTG
    ATTGAGGCCG GGGAGCAGCT GGGCTTCGAC TATGGGCAGC GCTTCTGGGA CATCAAGGGC
    AAGCTGTTCA GCTGCCGCTG CGGCTCCCCC AAGTGCCGGC ACTCCGGGGC CGCGCTGGCC
    CAGCGGCAGG CCGGTGCCCA GGAGGACGGG CTGCCCGACA CCAGCTCCTC GCCTGCCGAC
    CCGCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220434
    Clone ID Related Accession (Same CDS sequence) XM_024998812.1
    Accession Version XM_024998812.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCAGCTGC AGTATCTCGG
    AAGAAAAAGC GAAGAATGGG AACCTATAGC CTGGTTCCTA AGAAAAAGAC CAAAGTGTTA
    AAGCAGAGGA CGGTGATTGA GATGTTTAAG AGCATAACTC ATTCCACGGG GGGTTCCAAG
    GGTGAGAAGG ATCTGAGCGA TGGCTCCCTC CATGTGAACG GTGAGAGCCT GGATCTGGAC
    TCGGAGGAGG ATGACTCGGA GGAGCTGGAC GAGGATGAGG ACCAGGGCGG CCCACCAGCA
    GCTGCGTTCC CTACTGAGGA TGGCAGGGCC TCCAAGGATG GCGTGTTGGC GCCTGACTGC
    AGCCAGAAGG TGGACGGCGA GTCTGAAGAG GAGCAGGAGT CGGCCGGCAC CGCGGACGAG
    GAGGAGGACG GAGACGAGTC CGACTTGAGT TCTGAGTCCA GCGTCAAGAA GAAGTTCCTC
    AAGAGGAAGG GGAAGCCTGA CAGCCCTTGG ATCAAGCCAG CGAGGAAGAG GAGGCGGAGA
    AGCAAAAAGA AGCCCTGCTC TGTGTTAGGG CTGGCCAACG GTCCTGATGC AGGCGAGACG
    GATGGCCTCC AGGAGGTGCC CCTCTGCAGC TGCCGGATGG AGACCCCAAA GAGCCGGGAG
    ATCACCACTC TGGCCAACAA CCAGTGCATG GCCACAGAGA GTGTGGACCA CCAGCTGGGC
    AGGTGCACGA ACAGCGTGGT GAAGCATGAG CTGATGCGTC CGTCCAGCAA GGCACCGCTG
    CTGGTGCTGT GTGAGGACCA CCGGGGCCGG ATGGTGAAGC ACCAGTGCTG CCCCGGCTGC
    GGCTACTTCT GCACGGCCGG TAATTTTATG GAATGTCAGC CTGAGAGCAG CATTTCTCAT
    CGTTTCCACA AAGCCTGTGC CTCTCGAGTC AATAATGCCA GCTACTGTCC CCACTGTGGG
    GAGGAGACCT CCAAGGCCAA AGAGGTGACC ATTGCAAAGG CAGACACGAC GTCCACGGTG
    ACCCTGGCCC CTGGGCAGGA GAAGAGCTGT GCAGCAGAGG GCAGGGCCGA CACCACCACG
    GGCAGCGCTG CTGGCCCCCT GCTCTCAGAG GAGGACAAGC CGCAGAGCCT GGCCCCCCAG
    GTGCCGGAGG GCTTCGACCC CACAGGGCCG GCTGGGCTGG GGAAGCCGGC GCCAGGCCTC
    TCCCAGGGCC CTGGGAAGGA GACCCTGGAG AGCGCACTCA TCGCCCTGGA TTCCGAGAAA
    CCCAAGAAGC TCCGCTTCCA CCCGAAGCAG CTGTACTTCT CGGCGCGGCA AGGGGAGCTG
    CAGAAGGTGC TGCTCATGTT GGTTGACGGG ATCGACCCCA ACTTCAAGAT GGAGCATCAG
    AACAAGCGCT CGCCCCTGCA CGCCGCGGCG GAGGCTGGCC ACGTGGACAT CTGCCACATG
    CTGATCCAGG CGGGCGCCAA CATCGACACC TGCTCTGAGG ACCAGAGGAC CCCGCTGATG
    GAGGCCGCGG AGAACAACCA CTTGGACGCG GTGAAGTACC TCATCAAGGC CGGGGCCCTC
    GTGGATCCCA AGGACGCAGA GGGCTCGACC TGTTTGCACC TGGCTGCCAA GAAAGGCCAC
    TACGACGTGG TTCAGTACTT GCTCTCAAAC GGACAGATGG ACGTCAACTG TCAGGATGAT
    GGTGGCTGGA CGCCCATGAT CTGGGCCACT GAGTACAAGC ACGTGGACCT CGTGAAGCTG
    TTGCTCTCCA AGGGGTCTGA CATCAACATC CGGGACAACG AGGAAAACAT CTGCCTGCAC
    TGGGCGGCCT TCTCAGGCTG TGTGGACATC GCTGAGATCC TGCTGGCCGC CAGGTGCGAC
    CTGCACGCGG TGAACATCCA CGGGGACTCG CCCCTGCACA TCGCGGCCCG GGAGGACCGC
    TACGCCTGTG TCGTCCTCTT CCTGTCCCGG GACTCGGATG TCACTCTGAA GAACAAGGAG
    GGGGAGACTC CCCTGCAGTG CGCCAGCCTC AACTCACAGG TGTGGAGCGC CCTGCAGGTG
    AGCCAGGCGC TGCGGGACGC GGCCCCTGAC AGGCCCACCC CGGTGGAGAA GACGGTGAGC
    AGGGACATTG CTCGGGGCTA CGAGCGGATC CCCATCCCCT GCGTCAATGG TGTGGACGGC
    GAGCCGTGCC CCAGCAACTA CAAGTACGTG TCTCAGAACT GTGTGACGTC CCCCATGAAC
    ATCGATAGGA ACATCACCCA CCTGCAGTAC TGCGTGTGCA TCGACGACTG CTCCTCCAGC
    AACTGCATGT GTGGCCAGCT CAGCATGCGC TGCTGGTACG ACAAGGACGG TCGGCTGCTG
    CCAGAGTTCA ACATGGCAGA GCCGCCCCTG CTGTTCGAGT GCAACCACGC CTGCTCCTGC
    TGGAGGACGT GCCGCAACCG CGTGGTGCAG AATGGCCTCA GGGCGAGGCT GCAGCTCTAT
    CGGACACAGA ACATGGGCTG GGGCGTCCGG TCCCTGCAGG ACATCCCACT GGGCACCTTT
    GTTTGCGAGT ACGTTGGGGA GCTTATTTCT GACTCGGAAG CTGATGTGCG GGAAGAAGAC
    TCTTATCTCT TTGATCTTGA CAACAAGGAC GGAGAGCTGT ACTGCATCGA CGCGCGTTTC
    TATGGCAACG TCAGCCGCTT CATCAACCAC CACTGTGAGC CCAATCTGGT GCCCGTGCGT
    GTGTTCATGT CCCACCAGGA CCTGCGCTTC CCGCGGATCG CCTTCTTCAG CACCCGCCTG
    ATTGAGGCCG GGGAGCAGCT GGGCTTCGAC TATGGGCAGC GCTTCTGGGA CATCAAGGGC
    AAGCTGTTCA GCTGCCGCTG CGGCTCCCCC AAGTGCCGGC ACTCCGGGGC CGCGCTGGCC
    CAGCGGCAGG CCGGTGCCCA GGAGGACGGG CTGCCCGACA CCAGCTCCTC GCCTGCCGAC
    CCGCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854580.1
    CDS42..3770
    Translation

    Target ORF information:

    RefSeq Version XM_024998812.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCAGCTGC AGTATCTCGG
    AAGAAAAAGC GAAGAATGGG AACCTATAGC CTGGTTCCTA AGAAAAAGAC CAAAGTGTTA
    AAGCAGAGGA CGGTGATTGA GATGTTTAAG AGCATAACTC ATTCCACGGG GGGTTCCAAG
    GGTGAGAAGG ATCTGAGCGA TGGCTCCCTC CATGTGAACG GTGAGAGCCT GGATCTGGAC
    TCGGAGGAGG ATGACTCGGA GGAGCTGGAC GAGGATGAGG ACCAGGGCGG CCCACCAGCA
    GCTGCGTTCC CTACTGAGGA TGGCAGGGCC TCCAAGGATG GCGTGTTGGC GCCTGACTGC
    AGCCAGAAGG TGGACGGCGA GTCTGAAGAG GAGCAGGAGT CGGCCGGCAC CGCGGACGAG
    GAGGAGGACG GAGACGAGTC CGACTTGAGT TCTGAGTCCA GCGTCAAGAA GAAGTTCCTC
    AAGAGGAAGG GGAAGCCTGA CAGCCCTTGG ATCAAGCCAG CGAGGAAGAG GAGGCGGAGA
    AGCAAAAAGA AGCCCTGCTC TGTGTTAGGG CTGGCCAACG GTCCTGATGC AGGCGAGACG
    GATGGCCTCC AGGAGGTGCC CCTCTGCAGC TGCCGGATGG AGACCCCAAA GAGCCGGGAG
    ATCACCACTC TGGCCAACAA CCAGTGCATG GCCACAGAGA GTGTGGACCA CCAGCTGGGC
    AGGTGCACGA ACAGCGTGGT GAAGCATGAG CTGATGCGTC CGTCCAGCAA GGCACCGCTG
    CTGGTGCTGT GTGAGGACCA CCGGGGCCGG ATGGTGAAGC ACCAGTGCTG CCCCGGCTGC
    GGCTACTTCT GCACGGCCGG TAATTTTATG GAATGTCAGC CTGAGAGCAG CATTTCTCAT
    CGTTTCCACA AAGCCTGTGC CTCTCGAGTC AATAATGCCA GCTACTGTCC CCACTGTGGG
    GAGGAGACCT CCAAGGCCAA AGAGGTGACC ATTGCAAAGG CAGACACGAC GTCCACGGTG
    ACCCTGGCCC CTGGGCAGGA GAAGAGCTGT GCAGCAGAGG GCAGGGCCGA CACCACCACG
    GGCAGCGCTG CTGGCCCCCT GCTCTCAGAG GAGGACAAGC CGCAGAGCCT GGCCCCCCAG
    GTGCCGGAGG GCTTCGACCC CACAGGGCCG GCTGGGCTGG GGAAGCCGGC GCCAGGCCTC
    TCCCAGGGCC CTGGGAAGGA GACCCTGGAG AGCGCACTCA TCGCCCTGGA TTCCGAGAAA
    CCCAAGAAGC TCCGCTTCCA CCCGAAGCAG CTGTACTTCT CGGCGCGGCA AGGGGAGCTG
    CAGAAGGTGC TGCTCATGTT GGTTGACGGG ATCGACCCCA ACTTCAAGAT GGAGCATCAG
    AACAAGCGCT CGCCCCTGCA CGCCGCGGCG GAGGCTGGCC ACGTGGACAT CTGCCACATG
    CTGATCCAGG CGGGCGCCAA CATCGACACC TGCTCTGAGG ACCAGAGGAC CCCGCTGATG
    GAGGCCGCGG AGAACAACCA CTTGGACGCG GTGAAGTACC TCATCAAGGC CGGGGCCCTC
    GTGGATCCCA AGGACGCAGA GGGCTCGACC TGTTTGCACC TGGCTGCCAA GAAAGGCCAC
    TACGACGTGG TTCAGTACTT GCTCTCAAAC GGACAGATGG ACGTCAACTG TCAGGATGAT
    GGTGGCTGGA CGCCCATGAT CTGGGCCACT GAGTACAAGC ACGTGGACCT CGTGAAGCTG
    TTGCTCTCCA AGGGGTCTGA CATCAACATC CGGGACAACG AGGAAAACAT CTGCCTGCAC
    TGGGCGGCCT TCTCAGGCTG TGTGGACATC GCTGAGATCC TGCTGGCCGC CAGGTGCGAC
    CTGCACGCGG TGAACATCCA CGGGGACTCG CCCCTGCACA TCGCGGCCCG GGAGGACCGC
    TACGCCTGTG TCGTCCTCTT CCTGTCCCGG GACTCGGATG TCACTCTGAA GAACAAGGAG
    GGGGAGACTC CCCTGCAGTG CGCCAGCCTC AACTCACAGG TGTGGAGCGC CCTGCAGGTG
    AGCCAGGCGC TGCGGGACGC GGCCCCTGAC AGGCCCACCC CGGTGGAGAA GACGGTGAGC
    AGGGACATTG CTCGGGGCTA CGAGCGGATC CCCATCCCCT GCGTCAATGG TGTGGACGGC
    GAGCCGTGCC CCAGCAACTA CAAGTACGTG TCTCAGAACT GTGTGACGTC CCCCATGAAC
    ATCGATAGGA ACATCACCCA CCTGCAGTAC TGCGTGTGCA TCGACGACTG CTCCTCCAGC
    AACTGCATGT GTGGCCAGCT CAGCATGCGC TGCTGGTACG ACAAGGACGG TCGGCTGCTG
    CCAGAGTTCA ACATGGCAGA GCCGCCCCTG CTGTTCGAGT GCAACCACGC CTGCTCCTGC
    TGGAGGACGT GCCGCAACCG CGTGGTGCAG AATGGCCTCA GGGCGAGGCT GCAGCTCTAT
    CGGACACAGA ACATGGGCTG GGGCGTCCGG TCCCTGCAGG ACATCCCACT GGGCACCTTT
    GTTTGCGAGT ACGTTGGGGA GCTTATTTCT GACTCGGAAG CTGATGTGCG GGAAGAAGAC
    TCTTATCTCT TTGATCTTGA CAACAAGGAC GGAGAGCTGT ACTGCATCGA CGCGCGTTTC
    TATGGCAACG TCAGCCGCTT CATCAACCAC CACTGTGAGC CCAATCTGGT GCCCGTGCGT
    GTGTTCATGT CCCACCAGGA CCTGCGCTTC CCGCGGATCG CCTTCTTCAG CACCCGCCTG
    ATTGAGGCCG GGGAGCAGCT GGGCTTCGAC TATGGGCAGC GCTTCTGGGA CATCAAGGGC
    AAGCTGTTCA GCTGCCGCTG CGGCTCCCCC AAGTGCCGGC ACTCCGGGGC CGCGCTGGCC
    CAGCGGCAGG CCGGTGCCCA GGAGGACGGG CTGCCCGACA CCAGCTCCTC GCCTGCCGAC
    CCGCTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36669
    Clone ID Related Accession (Same CDS sequence) NM_001099041.1
    Accession Version NM_001099041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-11
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EHMT1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC142313.1. On Jun 26, 2007 this sequence version replaced XM_607033.3. ##Evidence-Data-START## Transcript exon combination :: BC142313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCAGCTGC AGTATCTCGG
    AAGAAAAAGC GAAGAATGGG AACCTATAGC CTGGTTCCTA AGAAGAAGAC CAAAGTGTTA
    AAGCAGAGGA CGGTGATTGA GATGTTTAAG AGCATAACTC ATTCCACGGG GGGTTCCAAG
    GGTGAGAAGG ATCTGAGCGA TGGCTCCCTC CATGTGAACG GTGAGAGCCT GGATCTGGAC
    TCGGAGGAGG ATGACTCGGA GGAGCTGGAC GAGGATGAGG ACCAGGGCGG CCCACCAGCA
    GCTGCGTTCC CTACTGAGGA TGGCAGGGCC TCCAAGGATG GCGTGTTGGC GCCTGACTGC
    AGCCAGAAGG TGGACGGCGA GTCTGAAGAG GAGCAGGAGT CGGCCGGCAC CGCGGACGAG
    GAGGAGGACG GAGACGAGTC CGACTTGAGT TCTGAGTCCA GCGTCAAGAA GAAGTTCCTC
    AAGAGGAAGG GGAAGCCTGA CAGCCCTTGG ATCAAGCCAG CGAGGAAGAG GAGGCGGAGA
    AGCAAAAAGA AGCCCTGCTC TGTGTTAGGT TCTGAAGCCT CTGCGTCGTC TTCGGGCAGT
    GTGGAGCCAA CGGCGCCTGG CGACGGCGGG GGGTACATGG AGGTGCCGCT GGACTCCTTG
    AATCTCCATG TCCAGGGTGC GCTGACCTCT GACACAGAAG GGCTGGCCAA CGGTCCTGAT
    GCAGGCGAGA CGGATGGCCT CCAGGAGGTG CCCCTCTGCA GCTGCCGGAT GGAGACCCCA
    AAGAGCCGGG AGATCACCAC TCTGGCCAAC AACCAGTGCA GGGCCACAGA GAGTGTGGAC
    CACCAGCTGG GCAGGTGCAC GAACAGCGTG GTGAAGCATG AGCTGATGCG TCCGTCCAGC
    AAGGCACCGC TGCTGGTGCT GTGTGAGGAC CACCGGGGCC GGATGGTGAA GCACCAGTGC
    TGCCCCGGCT GCGGCTACTT CTGCACGGCC GGTAATTTTA TGGAATGTCA GCCTGAGAGC
    AGCATTTCTC ATCGTTTCCA CAAAGCCTGT GCCTCTCGAG TCAATAATGC CAGCTACTGT
    CCCCACTGTG GGGAGGAGAC CTCCAAGGCC AAAGAGGTGA CCATTGCAAA GGCAGACACG
    ACGTCCACGG TGACCCTGGC CCCTGGGCAG GAGAAGAGCT GTGCAGCAGA GGGCAGGGCC
    GACACCACCA CGGGCAGCGC TGCTGGCCCC CTGCTCTCAG AGGAGGACAA GCCGCAGAGC
    CTGGCCCCCC AGGTGCCGGA GGGCTTCGAC CCCACAGGGC CGGCTGGGCT GGGGAAGCCG
    GCGCCAGGCC TCTCCCAGGG CCCTGGGAAG GAGACCCTGG AGAGCGCACT CATCGCCCTG
    GATTCCGAGA AACCCAAGAA GCTCCGCTTC CACCCGAAGC AGCTGTACTT CTCGGCGCGG
    CAAGGGGAGC TGCAGAAGGT GCTGCTCATG TTGGTTGACG GGATCGACCC CAACTTCAAG
    ATGGAGCATC AGAACAAGCG CTCGCCCCTG CACGCCGCGG CGGAGGTTGG CCACGTGGAC
    ATCTGCCACA TGCTGATCCA GGCGGGCGCC AACATCGACA CCTGCTCTGA GGACCAGAGG
    ACCCCGCTGA TGGAGGCCGC GGAGAACAAC CACTTGGACG CGGTGAAGTA CCTCATCAAG
    GCCGGGGCCC TCGTGGATCC CAAGGACGCA GAGGGCTCGA CCTGTTTGCA CCTGGCTGCC
    AAGAAAGGCC ACTACGACGT GGTTCAGTAC TTGCTCTCAA ACGGACAGAT GGACGTCAAC
    TGTCAGGATG ATGGTGGCTG GACGCCCATG ATCTGGGCCA CTGAGTACAA GCACGTGGAC
    CTCGTGAAGC TGTTGCTCTC CAAGGGGTCT GACATCAACA TCCGGGACAA CGAGGAAAAC
    ATCTGCCTGC ACTGGGCGGC CTTCTCAGGC TGTGTGGACA TCGCTGAGAT CCTGCTGGCC
    GCCAGGTGCG ACCTGCACGC GGTGAACATC CACGGGGACT CGCCCCTGCA CATCGCGGCC
    CGGGAGGACC GCTACGCCTG TGTCGTCCTC TTCCTGTCCC GGGACTCGGA TGTCACTCTG
    AAGAACAAGG AGGGGGAGAC TCCCCTGCAG TGCGCCAGCC TCAACTCACA GGTGTGGAGC
    GCCCTGCAGG TGAGCCAGGC GCTGCGGGAC GCGGCCCCTG ACAGGCCCAC CCCGGTGGAG
    AAGACGGTGA GCAGGGACAT TGCTCGGGGC TACGAGCGGA TCCCCATCCC CTGCGTCAAT
    GGTGTGGACG GCGAGCCGTG CCCCAGCAAC TACAAGTACG TGTCTCAGAA CTGTGTGACG
    TCCCCCATGA ACATCGATAG GAACATCACC CACCTGCAGT ACTGCGTGTG CATCGACGAC
    TGCTCCTCCA GCAACTGCAT GTGTGGCCAG CTCAGCATGC GCTGCTGGTA CGACAAGGAC
    GGTCGGCTGC TGCCAGAGTT CAACATGGCA GAGCCGCCCC TGCTGTTCGA GTGCAACCAC
    GCCTGCTCCT GCTGGAGGAC GTGCCGCAAC CGCGTGGTGC AGAATGGCCT CAGGGCGAGG
    CTGCAGCTCT ATCGGACACA GAACATGGGC TGGGGCGTCC GGTCCCTGCA GGACATCCCA
    CTGGGCACCT TTGTTTGCGA GTACGTTGGG GAGCTTATTT CTGACTCGGA AGCTGATGTG
    CGGGAAGAAG ACTCTTATCT CTTTGATCTT GACAACAAGG ACGGAGAGCT GTACTGCATC
    GACGCGCGTT TCTATGGCAA CGTCAGCCGC TTCATCAACC ACCACTGTGA GCCCAATCTG
    GTGCCCGTGC GTGTGTTCAT GTCCCACCAG GACCTGCGCT TCCCGCGGAT CGCCTTCTTC
    AGCACCCGCC TGATTGAGGC CGGGGAGCAG CTGGGCTTCG ACTATGGGCA GCGCTTCTGG
    GACATCAAGG GCAAGCTGTT CAGCTGCCGC TGCGGCTCCC CCAAGTGCCG GCACTCCGGG
    GCCGCGCTGG CCCAGCGGCA GGCCGGTGCC CAGGAGGACG GGCTGCCCGA CACCAGCTCC
    TCGCCTGCCG ACCCGCTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001092511.1
    CDS15..3875
    Translation

    Target ORF information:

    RefSeq Version NM_001099041.1
    Organism Bos taurus(cattle)
    Definition Bos taurus euchromatic histone lysine methyltransferase 1 (EHMT1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001099041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGCCG CCGATGCCGA GGCGGTTCCG GCGAAGGGGG AGACCAAGCA GGACTGCGGC 
    CTGGGAGCTG AGCTGCCGAG CGAAGGGTCA CCCATGGCTG CTGATGACGG CTCAGCGGAA
    CAGCAGGGAG GGGAGGCCCC GGTGGCTGCA GACGGGGAGA CCAACGGGTC CTGTGACCAG
    AGCGATGCCA GTGGCCACGC GGCTGCTGTG AAGCACCCTC AGGAGAGCAC ACGAGGCAGC
    CCTCAGGAAG GGGCCGGCCC GGGGTCCCGG ATAGCGGAAA ATGGGATTTT GGACAGAGAC
    CCCGAGGTGG GGAAGCAGAA CCACATCCGC TCTGATGGCT TCCCCCAGAA TCCTGTCATT
    GGGAGCAACG GCTACTTCCT TGGCAAGGCC CCCTTGTCTG AGCCACCACT GCGGGTGGCC
    AGCGCCCTGG CTTCCTCCCT GCCTGGCCAC GCGGCGAAGA CCCTCCCTGG CGGGGCTGGC
    AAAGGGAGGA CTCCCGGTGC TTTTCCCCAG CCGCCGGCTG CTGTGCCGGC CAAGCCTGGG
    GAGGGCAGCA AGGACACAGA GGACAAGAAG GCCCCGGCCC CGGGCGCCGA CGTCAAGGTC
    CACAGGGCTC GGAAGACCAT GCCGAAGTCC ATCCCGGGCC TGCACGCGGC GAGTAAGGAC
    CCCAGGGAAG GCCGGGACGC CCGCGACCAC GAGGAGCCGA AGGAGGAGCT GAGCAGGGGC
    GCCTCGGAGT TCGCACGCCA GCAGCTTTCA CCCCCCTTCC CGTCCCTTCA CCAGTCGCTA
    CCTCAGAACC AATGCTACGT GGCCACCGCG AAGGCCCAGA CAGCAGCTGC AGTATCTCGG
    AAGAAAAAGC GAAGAATGGG AACCTATAGC CTGGTTCCTA AGAAGAAGAC CAAAGTGTTA
    AAGCAGAGGA CGGTGATTGA GATGTTTAAG AGCATAACTC ATTCCACGGG GGGTTCCAAG
    GGTGAGAAGG ATCTGAGCGA TGGCTCCCTC CATGTGAACG GTGAGAGCCT GGATCTGGAC
    TCGGAGGAGG ATGACTCGGA GGAGCTGGAC GAGGATGAGG ACCAGGGCGG CCCACCAGCA
    GCTGCGTTCC CTACTGAGGA TGGCAGGGCC TCCAAGGATG GCGTGTTGGC GCCTGACTGC
    AGCCAGAAGG TGGACGGCGA GTCTGAAGAG GAGCAGGAGT CGGCCGGCAC CGCGGACGAG
    GAGGAGGACG GAGACGAGTC CGACTTGAGT TCTGAGTCCA GCGTCAAGAA GAAGTTCCTC
    AAGAGGAAGG GGAAGCCTGA CAGCCCTTGG ATCAAGCCAG CGAGGAAGAG GAGGCGGAGA
    AGCAAAAAGA AGCCCTGCTC TGTGTTAGGT TCTGAAGCCT CTGCGTCGTC TTCGGGCAGT
    GTGGAGCCAA CGGCGCCTGG CGACGGCGGG GGGTACATGG AGGTGCCGCT GGACTCCTTG
    AATCTCCATG TCCAGGGTGC GCTGACCTCT GACACAGAAG GGCTGGCCAA CGGTCCTGAT
    GCAGGCGAGA CGGATGGCCT CCAGGAGGTG CCCCTCTGCA GCTGCCGGAT GGAGACCCCA
    AAGAGCCGGG AGATCACCAC TCTGGCCAAC AACCAGTGCA GGGCCACAGA GAGTGTGGAC
    CACCAGCTGG GCAGGTGCAC GAACAGCGTG GTGAAGCATG AGCTGATGCG TCCGTCCAGC
    AAGGCACCGC TGCTGGTGCT GTGTGAGGAC CACCGGGGCC GGATGGTGAA GCACCAGTGC
    TGCCCCGGCT GCGGCTACTT CTGCACGGCC GGTAATTTTA TGGAATGTCA GCCTGAGAGC
    AGCATTTCTC ATCGTTTCCA CAAAGCCTGT GCCTCTCGAG TCAATAATGC CAGCTACTGT
    CCCCACTGTG GGGAGGAGAC CTCCAAGGCC AAAGAGGTGA CCATTGCAAA GGCAGACACG
    ACGTCCACGG TGACCCTGGC CCCTGGGCAG GAGAAGAGCT GTGCAGCAGA GGGCAGGGCC
    GACACCACCA CGGGCAGCGC TGCTGGCCCC CTGCTCTCAG AGGAGGACAA GCCGCAGAGC
    CTGGCCCCCC AGGTGCCGGA GGGCTTCGAC CCCACAGGGC CGGCTGGGCT GGGGAAGCCG
    GCGCCAGGCC TCTCCCAGGG CCCTGGGAAG GAGACCCTGG AGAGCGCACT CATCGCCCTG
    GATTCCGAGA AACCCAAGAA GCTCCGCTTC CACCCGAAGC AGCTGTACTT CTCGGCGCGG
    CAAGGGGAGC TGCAGAAGGT GCTGCTCATG TTGGTTGACG GGATCGACCC CAACTTCAAG
    ATGGAGCATC AGAACAAGCG CTCGCCCCTG CACGCCGCGG CGGAGGTTGG CCACGTGGAC
    ATCTGCCACA TGCTGATCCA GGCGGGCGCC AACATCGACA CCTGCTCTGA GGACCAGAGG
    ACCCCGCTGA TGGAGGCCGC GGAGAACAAC CACTTGGACG CGGTGAAGTA CCTCATCAAG
    GCCGGGGCCC TCGTGGATCC CAAGGACGCA GAGGGCTCGA CCTGTTTGCA CCTGGCTGCC
    AAGAAAGGCC ACTACGACGT GGTTCAGTAC TTGCTCTCAA ACGGACAGAT GGACGTCAAC
    TGTCAGGATG ATGGTGGCTG GACGCCCATG ATCTGGGCCA CTGAGTACAA GCACGTGGAC
    CTCGTGAAGC TGTTGCTCTC CAAGGGGTCT GACATCAACA TCCGGGACAA CGAGGAAAAC
    ATCTGCCTGC ACTGGGCGGC CTTCTCAGGC TGTGTGGACA TCGCTGAGAT CCTGCTGGCC
    GCCAGGTGCG ACCTGCACGC GGTGAACATC CACGGGGACT CGCCCCTGCA CATCGCGGCC
    CGGGAGGACC GCTACGCCTG TGTCGTCCTC TTCCTGTCCC GGGACTCGGA TGTCACTCTG
    AAGAACAAGG AGGGGGAGAC TCCCCTGCAG TGCGCCAGCC TCAACTCACA GGTGTGGAGC
    GCCCTGCAGG TGAGCCAGGC GCTGCGGGAC GCGGCCCCTG ACAGGCCCAC CCCGGTGGAG
    AAGACGGTGA GCAGGGACAT TGCTCGGGGC TACGAGCGGA TCCCCATCCC CTGCGTCAAT
    GGTGTGGACG GCGAGCCGTG CCCCAGCAAC TACAAGTACG TGTCTCAGAA CTGTGTGACG
    TCCCCCATGA ACATCGATAG GAACATCACC CACCTGCAGT ACTGCGTGTG CATCGACGAC
    TGCTCCTCCA GCAACTGCAT GTGTGGCCAG CTCAGCATGC GCTGCTGGTA CGACAAGGAC
    GGTCGGCTGC TGCCAGAGTT CAACATGGCA GAGCCGCCCC TGCTGTTCGA GTGCAACCAC
    GCCTGCTCCT GCTGGAGGAC GTGCCGCAAC CGCGTGGTGC AGAATGGCCT CAGGGCGAGG
    CTGCAGCTCT ATCGGACACA GAACATGGGC TGGGGCGTCC GGTCCCTGCA GGACATCCCA
    CTGGGCACCT TTGTTTGCGA GTACGTTGGG GAGCTTATTT CTGACTCGGA AGCTGATGTG
    CGGGAAGAAG ACTCTTATCT CTTTGATCTT GACAACAAGG ACGGAGAGCT GTACTGCATC
    GACGCGCGTT TCTATGGCAA CGTCAGCCGC TTCATCAACC ACCACTGTGA GCCCAATCTG
    GTGCCCGTGC GTGTGTTCAT GTCCCACCAG GACCTGCGCT TCCCGCGGAT CGCCTTCTTC
    AGCACCCGCC TGATTGAGGC CGGGGAGCAG CTGGGCTTCG ACTATGGGCA GCGCTTCTGG
    GACATCAAGG GCAAGCTGTT CAGCTGCCGC TGCGGCTCCC CCAAGTGCCG GCACTCCGGG
    GCCGCGCTGG CCCAGCGGCA GGCCGGTGCC CAGGAGGACG GGCTGCCCGA CACCAGCTCC
    TCGCCTGCCG ACCCGCTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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