NAGPA cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAGPA
Entrez Gene ID	106897805
Full Name	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014958683.1	XP_014814169.1	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Pathways from BioSystems
Gene Ontology

The following NAGPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAGPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa77265	XM_014958683.1 Latest version!	Calidris pugnax N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAGPA ( XM_014958683.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014814169.1 Calidris pugnax N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.

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CloneID	OCa77265
Clone ID Related Accession (Same CDS sequence)	XM_014958683.1
Accession Version	XM_014958683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091141.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGGCGGCC AAGCGCCCGC CAAGATGGCG GCCTGCGGGG CGGCGGGGCC CGCGGGCGGG 
GGCTGGCTGC AGGCGGTGCG GGGCGCCGGG AGCCCCCCCA GCGACCCTCT GCTGCAGCCC 
TATTTCCCCT CCCAGCACGG CCCTCGGCAC CACCACAGGT ACGTCAGAGA CTGTCAGCCC 
ATCAAGTATG GCAACGTAAC ACACGAAGCT TGGCCCAGCG ACAACAGGAC GGATGGCCCG 
GTGGCTACCA CCAGAACGTT TGTTTCTTAC ATCCCCCCGG ATGGCGAGAA CCGCAAGGTG 
GTCTATGGCC ACTTCACTTT CGTGAGGAAC CCCCTGAGGA CCTTCTCTGT GCTAGAGCCG 
GGGGGCACAG GAGGCTGCCA GGCTCAACGC AGAGCCACCG TGGAAGAGAC CGCAAGGCTC 
AGGAAGTGTC TGGTGGCCCA GAACGGTGGG TACTTCAACA TGGAAACTGG AGAGTGCCTT 
GGGAATGTTG TGAGTGATGG GAAGCTGGTG AGAAACTCTG GAGGCCTGCA GAACGCTCAG 
TTCGGCATCC GGAAGGATGG CACCATGGTG TTTGGTTACC TGTCGGAGGA AGACGTCTTG 
GATCAAGCAA ACCCTTTTGT GCAGCTCGTG AGCGGGGTGG TTTGGCTGCT GAGGGATGGA 
GAGGTGTACG TCAGCCAGAG CCGCGTGGCT GAGTGCGGGG ACGTTCAAAC CACAGGAACC 
TTCGACAGGT TCATCAACGT GATCTCGGCC AGGACGGCGG TGGGACACGA CAGTCAGGGG 
CAGCTGGTCC TGGTTCACGT GGATGGACAG ACAGAATCCA GAGGGGTCAA CCTCTGGGAA 
ATGGCTGAAT TTCTGAAGAA GCAGGGAATC ATCAACGCCA TCAACCTGGA CGGTGGAGGG 
TCTGCAACAC TGGTCTTAAA TGGGACCCTC ACAAGCTACC CATCTGAGCA CTGCTCCTTC 
GACAGCATGT GGCGGTGCCC GCGCAGCATC TCCACCGTCC TCTGCGTCCA CGAGCCCCCC 
TGCCAGCCCC CTGACTGCAG CGGCCACGGG CACTGCGTGG CAGGGACCTG CCGCTGCTCC 
GGGGACTTCT GGAGGGGCCC AGCCTGTGAC ATCTTGGACT GTGGCCCTTC CAACTGCAGC 
CTGCGCGGGG TCTGCACCGA CGCTGGATGC CTCTGTGACG CTGGCTGGAC CGGCAGCAAC 
TGCAGCCAAG CTTGTCCCAG TGGTTTCTAC GGGGCCGCCT GCGCCCAGAG ATGCCTGTGC 
CAGAACGGTG GCTCCTGCGA CCCCGTGCAT GGGGCCTGCT CCTGCCCGGC TGGGTACCAC 
GGCACCAGCT GTGAGCGAGA GTGTCCCATG GGCTGGTACG GGCCAAACTG CCGGGAGCAG 
TGTGTGTGTG AACACTCGTG TCCCTGCGAC CGGGAGACGG GCAGCTGCAA CGTCACCTTC 
CCGCCCGCAG TGCAGGAGCA GCTCAACAAA GCTGGGCGGT GTTTGGCTTC CCAGGGTAAG 
GGAAGGAGCC AAGACAAGTT CTCTCTGTCA GAAAAAACCT GGCTCTCCAT GACCTCTGTC 
TTGGCTCTGC TGCTGGTGAT CAGCGCCATG GGCAACGCGG GCCTTCTCCT GAAGGCCAGG 
TCAGAGAGGC AGCAGGAGAG CGGCGACTAT CTCTACCTGC CGCTGAGGGA GATGAACGGG 
GAAGCCGGTC ACACCTCCAC CTCCTGCCAG ATGGACGATG CCCAGGACCA GAACCAGGCC 
CTCCTTCAGA GCCCCGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014814169.1
CDS	1..1761
Translation	MGGQAPAKMAACGAAGPAGGGWLQAVRGAGSPPSDPLLQPYFPSQHGPRHHHRYVRDCQPIKYGNVTHEAWPSDNRTDGPVATTRTFVSYIPPDGENRKVVYGHFTFVRNPLRTFSVLEPGGTGGCQAQRRATVEETARLRKCLVAQNGGYFNMETGECLGNVVSDGKLVRNSGGLQNAQFGIRKDGTMVFGYLSEEDVLDQANPFVQLVSGVVWLLRDGEVYVSQSRVAECGDVQTTGTFDRFINVISARTAVGHDSQGQLVLVHVDGQTESRGVNLWEMAEFLKKQGIINAINLDGGGSATLVLNGTLTSYPSEHCSFDSMWRCPRSISTVLCVHEPPCQPPDCSGHGHCVAGTCRCSGDFWRGPACDILDCGPSNCSLRGVCTDAGCLCDAGWTGSNCSQACPSGFYGAACAQRCLCQNGGSCDPVHGACSCPAGYHGTSCERECPMGWYGPNCREQCVCEHSCPCDRETGSCNVTFPPAVQEQLNKAGRCLASQGKGRSQDKFSLSEKTWLSMTSVLALLLVISAMGNAGLLLKARSERQQESGDYLYLPLREMNGEAGHTSTSCQMDDAQDQNQALLQSPA

Target ORF information:

RefSeq Version	XM_014958683.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014958683.1

ORF Insert Sequence:

ATGGGCGGCC AAGCGCCCGC CAAGATGGCG GCCTGCGGGG CGGCGGGGCC CGCGGGCGGG 
GGCTGGCTGC AGGCGGTGCG GGGCGCCGGG AGCCCCCCCA GCGACCCTCT GCTGCAGCCC 
TATTTCCCCT CCCAGCACGG CCCTCGGCAC CACCACAGGT ACGTCAGAGA CTGTCAGCCC 
ATCAAGTATG GCAACGTAAC ACACGAAGCT TGGCCCAGCG ACAACAGGAC GGATGGCCCG 
GTGGCTACCA CCAGAACGTT TGTTTCTTAC ATCCCCCCGG ATGGCGAGAA CCGCAAGGTG 
GTCTATGGCC ACTTCACTTT CGTGAGGAAC CCCCTGAGGA CCTTCTCTGT GCTAGAGCCG 
GGGGGCACAG GAGGCTGCCA GGCTCAACGC AGAGCCACCG TGGAAGAGAC CGCAAGGCTC 
AGGAAGTGTC TGGTGGCCCA GAACGGTGGG TACTTCAACA TGGAAACTGG AGAGTGCCTT 
GGGAATGTTG TGAGTGATGG GAAGCTGGTG AGAAACTCTG GAGGCCTGCA GAACGCTCAG 
TTCGGCATCC GGAAGGATGG CACCATGGTG TTTGGTTACC TGTCGGAGGA AGACGTCTTG 
GATCAAGCAA ACCCTTTTGT GCAGCTCGTG AGCGGGGTGG TTTGGCTGCT GAGGGATGGA 
GAGGTGTACG TCAGCCAGAG CCGCGTGGCT GAGTGCGGGG ACGTTCAAAC CACAGGAACC 
TTCGACAGGT TCATCAACGT GATCTCGGCC AGGACGGCGG TGGGACACGA CAGTCAGGGG 
CAGCTGGTCC TGGTTCACGT GGATGGACAG ACAGAATCCA GAGGGGTCAA CCTCTGGGAA 
ATGGCTGAAT TTCTGAAGAA GCAGGGAATC ATCAACGCCA TCAACCTGGA CGGTGGAGGG 
TCTGCAACAC TGGTCTTAAA TGGGACCCTC ACAAGCTACC CATCTGAGCA CTGCTCCTTC 
GACAGCATGT GGCGGTGCCC GCGCAGCATC TCCACCGTCC TCTGCGTCCA CGAGCCCCCC 
TGCCAGCCCC CTGACTGCAG CGGCCACGGG CACTGCGTGG CAGGGACCTG CCGCTGCTCC 
GGGGACTTCT GGAGGGGCCC AGCCTGTGAC ATCTTGGACT GTGGCCCTTC CAACTGCAGC 
CTGCGCGGGG TCTGCACCGA CGCTGGATGC CTCTGTGACG CTGGCTGGAC CGGCAGCAAC 
TGCAGCCAAG CTTGTCCCAG TGGTTTCTAC GGGGCCGCCT GCGCCCAGAG ATGCCTGTGC 
CAGAACGGTG GCTCCTGCGA CCCCGTGCAT GGGGCCTGCT CCTGCCCGGC TGGGTACCAC 
GGCACCAGCT GTGAGCGAGA GTGTCCCATG GGCTGGTACG GGCCAAACTG CCGGGAGCAG 
TGTGTGTGTG AACACTCGTG TCCCTGCGAC CGGGAGACGG GCAGCTGCAA CGTCACCTTC 
CCGCCCGCAG TGCAGGAGCA GCTCAACAAA GCTGGGCGGT GTTTGGCTTC CCAGGGTAAG 
GGAAGGAGCC AAGACAAGTT CTCTCTGTCA GAAAAAACCT GGCTCTCCAT GACCTCTGTC 
TTGGCTCTGC TGCTGGTGAT CAGCGCCATG GGCAACGCGG GCCTTCTCCT GAAGGCCAGG 
TCAGAGAGGC AGCAGGAGAG CGGCGACTAT CTCTACCTGC CGCTGAGGGA GATGAACGGG 
GAAGCCGGTC ACACCTCCAC CTCCTGCCAG ATGGACGATG CCCAGGACCA GAACCAGGCC 
CTCCTTCAGA GCCCCGCATG A