NFX1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFX1
Entrez Gene ID	481581
Full Name	nuclear transcription factor, X-box binding 1
General protein information	Preferred Names nuclear transcription factor, X-box binding 1 Names transcriptional repressor NF-X1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_860463.5	XP_865556.2	transcriptional repressor NF-X1 isoform X2
XM_005626706.2	XP_005626763.1	transcriptional repressor NF-X1 isoform X1
XM_860463.4	XP_865556.2	transcriptional repressor NF-X1 isoform X2
XM_005626706.3	XP_005626763.1	transcriptional repressor NF-X1 isoform X1
XM_005626707.2	XP_005626764.1	transcriptional repressor NF-X1 isoform X3
XM_022425500.1	XP_022281208.1	transcriptional repressor NF-X1 isoform X3
XM_005626707.3	XP_005626764.1	transcriptional repressor NF-X1 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFX1	XP_001159552.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008728	XP_314840.4
Oryza sativa (rice)	Os06g0252300	NP_001057298.1
Bos taurus (cattle)	NFX1	NP_001095499.1
Rattus norvegicus (Norway rat)	Nfx1	NP_001019955.2
Drosophila melanogaster (fruit fly)	stc	NP_001260460.1
Kluyveromyces lactis	KLLA0E08317g	XP_454325.1
Xenopus tropicalis (tropical clawed frog)	nfx1	XP_002935327.2
Homo sapiens (human)	NFX1	NP_002495.2
Eremothecium gossypii	AGOS_ADL213W	NP_983883.2
Magnaporthe oryzae (rice blast fungus)	MGG_00137	XP_003718994.1
Arabidopsis thaliana (thale cress)	NFXL1	NP_172488.1
Gallus gallus (chicken)	NFX1	XP_004935125.1
Saccharomyces cerevisiae (baker's yeast)	FAP1	NP_014375.1
Schizosaccharomyces pombe (fission yeast)	SPCC18.03	NP_588382.1
Canis lupus familiaris (dog)	NFX1	XP_005626763.1
Mus musculus (house mouse)	Nfx1	NP_076228.2
Danio rerio (zebrafish)	LOC562071	XP_690559.4
Caenorhabditis elegans (roundworm)	nfx-1	NP_498394.1

Gene Ontology

The following NFX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe292106	XM_022425500.1 Latest version!	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe14497	XM_005626706.3 Latest version!	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe14498	XM_860463.5 Latest version!	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe14499	XM_005626707.3 Latest version!	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe14498	XM_860463.4	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe14497	XM_005626706.2	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe14499	XM_005626707.2	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFX1 ( XM_022425500.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281208.1 Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe292106
Clone ID Related Accession (Same CDS sequence)	XM_022425500.1
Accession Version	XM_022425500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcriptional repressor NF-X1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGGCTGGAG TGTGATGAGG AGTGTTTAGC CTTGGAAAGG 
AAAAAGCGGT TAGCAGAGGC ATTTCATATT AGTGAGGATT CTGATCCTTT CAATGTACGT 
TCTTCGGGGT CAAAATTCAG TGACAGTTTA AAAGATGATG CCAGGAAGGA TTTAAAGTTT 
GTCAGTGACA TTGAAAAAGA AATGGAAGCC CTTGTGGAGG CCGTGAATAA GGGAAAGAAT 
AGTAAGAAGA GCCATTGCTT CCCTCCCATG AACAGAGACC ACCGCCGGAT CATCCACGAC 
CTGGCCCAAG TGTATGGCCT GGAGAGCGTG AGCTATGACA GTGAACCAAA GCGCAATGTT 
GTCGTCACTG CAATCAGGGG AAAGTCCATT TGTCCTCCTA CCACGCTGAT TGGTGTCCTT 
GAAAGGGAAA TGCAGTCACG GCCTCCACCA CCAATTCCTC ATCACAGACA TCAGACAGAC 
AAGAGTCCTG TGAGCAGTAA TTTACAGAAA ATAGCCAAGG AACCAGTAAT TGACTACTTT 
GATGTCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281208.1
CDS	516..3770
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGSQRRLDSNRIGRRNYSSPPPCHLSRQVPYDDISAVHQHSYPSGSKPKSQQISFQSSASNKSLKSHGLQNQPWQKLRSEKQHIKVKKVQNLTDQTSDAAGLESVARSESGTNPREHSPSENEKEVIGADPRGAKPKKAAQFIYSYGRGPKVKGKLKCEWGNRMTPKSEDAVPENTKPVGVIHPDSLDTSFKKSVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGQRHTNATRNNMAPIPKDNLNERPAKSACDNGNLAGVNKSSRRVDPEKCAVRRQDPQVTSFPRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENLLNCGQHHCAELCHGGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLCQLLELGSSTRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQSCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHELRSNIPCHLADISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHPSSPCPMTACKAKIELQCECGRRKEMMICSEASSTYQRLECDEECLALERKKRLAEAFHISEDSDPFNVRSSGSKFSDSLKDDARKDLKFVSDIEKEMEALVEAVNKGKNSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSICPPTTLIGVLEREMQSRPPPPIPHHRHQTDKSPVSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_022425500.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425500.1

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGGCTGGAG TGTGATGAGG AGTGTTTAGC CTTGGAAAGG 
AAAAAGCGGT TAGCAGAGGC ATTTCATATT AGTGAGGATT CTGATCCTTT CAATGTACGT 
TCTTCGGGGT CAAAATTCAG TGACAGTTTA AAAGATGATG CCAGGAAGGA TTTAAAGTTT 
GTCAGTGACA TTGAAAAAGA AATGGAAGCC CTTGTGGAGG CCGTGAATAA GGGAAAGAAT 
AGTAAGAAGA GCCATTGCTT CCCTCCCATG AACAGAGACC ACCGCCGGAT CATCCACGAC 
CTGGCCCAAG TGTATGGCCT GGAGAGCGTG AGCTATGACA GTGAACCAAA GCGCAATGTT 
GTCGTCACTG CAATCAGGGG AAAGTCCATT TGTCCTCCTA CCACGCTGAT TGGTGTCCTT 
GAAAGGGAAA TGCAGTCACG GCCTCCACCA CCAATTCCTC ATCACAGACA TCAGACAGAC 
AAGAGTCCTG TGAGCAGTAA TTTACAGAAA ATAGCCAAGG AACCAGTAAT TGACTACTTT 
GATGTCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005626706.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626763.1 Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14497
Clone ID Related Accession (Same CDS sequence)	XM_005626706.2 , XM_005626706.3
Accession Version	XM_005626706.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcriptional repressor NF-X1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCTAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAAATCAGC AAGTTAATTA CTAAGAAAGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAGTGTTTA GCCTTGGAAA GGAAAAAGCG GTTAGCAGAG 
GCATTTCATA TTAGTGAGGA TTCTGATCCT TTCAATGTAC GTTCTTCGGG GTCAAAATTC 
AGTGACAGTT TAAAAGATGA TGCCAGGAAG GATTTAAAGT TTGTCAGTGA CATTGAAAAA 
GAAATGGAAG CCCTTGTGGA GGCCGTGAAT AAGGGAAAGA ATAGTAAGAA GAGCCATTGC 
TTCCCTCCCA TGAACAGAGA CCACCGCCGG ATCATCCACG ACCTGGCCCA AGTGTATGGC 
CTGGAGAGCG TGAGCTATGA CAGTGAACCA AAGCGCAATG TTGTCGTCAC TGCAATCAGG 
GGAAAGTCCA TTTGTCCTCC TACCACGCTG ATTGGTGTCC TTGAAAGGGA AATGCAGTCA 
CGGCCTCCAC CACCAATTCC TCATCACAGA CATCAGACAG ACAAGAGTCC TGTGAGCAGT 
AATTTACAGA AAATAGCCAA GGAACCAGTA ATTGACTACT TTGATGTCCA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626763.1
CDS	516..3872
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGSQRRLDSNRIGRRNYSSPPPCHLSRQVPYDDISAVHQHSYPSGSKPKSQQISFQSSASNKSLKSHGLQNQPWQKLRSEKQHIKVKKVQNLTDQTSDAAGLESVARSESGTNPREHSPSENEKEVIGADPRGAKPKKAAQFIYSYGRGPKVKGKLKCEWGNRMTPKSEDAVPENTKPVGVIHPDSLDTSFKKSVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGQRHTNATRNNMAPIPKDNLNERPAKSACDNGNLAGVNKSSRRVDPEKCAVRRQDPQVTSFPRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENLLNCGQHHCAELCHGGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLCQLLELGSSTRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQSCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHELRSNIPCHLADISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHPSSPCPMTACKAKIELQCECGRRKEMMICSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECLALERKKRLAEAFHISEDSDPFNVRSSGSKFSDSLKDDARKDLKFVSDIEKEMEALVEAVNKGKNSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSICPPTTLIGVLEREMQSRPPPPIPHHRHQTDKSPVSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_005626706.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626706.2

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCTAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAAATCAGC AAGTTAATTA CTAAGAAAGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAGTGTTTA GCCTTGGAAA GGAAAAAGCG GTTAGCAGAG 
GCATTTCATA TTAGTGAGGA TTCTGATCCT TTCAATGTAC GTTCTTCGGG GTCAAAATTC 
AGTGACAGTT TAAAAGATGA TGCCAGGAAG GATTTAAAGT TTGTCAGTGA CATTGAAAAA 
GAAATGGAAG CCCTTGTGGA GGCCGTGAAT AAGGGAAAGA ATAGTAAGAA GAGCCATTGC 
TTCCCTCCCA TGAACAGAGA CCACCGCCGG ATCATCCACG ACCTGGCCCA AGTGTATGGC 
CTGGAGAGCG TGAGCTATGA CAGTGAACCA AAGCGCAATG TTGTCGTCAC TGCAATCAGG 
GGAAAGTCCA TTTGTCCTCC TACCACGCTG ATTGGTGTCC TTGAAAGGGA AATGCAGTCA 
CGGCCTCCAC CACCAATTCC TCATCACAGA CATCAGACAG ACAAGAGTCC TGTGAGCAGT 
AATTTACAGA AAATAGCCAA GGAACCAGTA ATTGACTACT TTGATGTCCA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005626706.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626763.1 Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.

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CloneID	OCe14497
Clone ID Related Accession (Same CDS sequence)	XM_005626706.2 , XM_005626706.3
Accession Version	XM_005626706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcriptional repressor NF-X1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCTAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAAATCAGC AAGTTAATTA CTAAGAAAGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAGTGTTTA GCCTTGGAAA GGAAAAAGCG GTTAGCAGAG 
GCATTTCATA TTAGTGAGGA TTCTGATCCT TTCAATGTAC GTTCTTCGGG GTCAAAATTC 
AGTGACAGTT TAAAAGATGA TGCCAGGAAG GATTTAAAGT TTGTCAGTGA CATTGAAAAA 
GAAATGGAAG CCCTTGTGGA GGCCGTGAAT AAGGGAAAGA ATAGTAAGAA GAGCCATTGC 
TTCCCTCCCA TGAACAGAGA CCACCGCCGG ATCATCCACG ACCTGGCCCA AGTGTATGGC 
CTGGAGAGCG TGAGCTATGA CAGTGAACCA AAGCGCAATG TTGTCGTCAC TGCAATCAGG 
GGAAAGTCCA TTTGTCCTCC TACCACGCTG ATTGGTGTCC TTGAAAGGGA AATGCAGTCA 
CGGCCTCCAC CACCAATTCC TCATCACAGA CATCAGACAG ACAAGAGTCC TGTGAGCAGT 
AATTTACAGA AAATAGCCAA GGAACCAGTA ATTGACTACT TTGATGTCCA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626763.1
CDS	1..3357
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGSQRRLDSNRIGRRNYSSPPPCHLSRQVPYDDISAVHQHSYPSGSKPKSQQISFQSSASNKSLKSHGLQNQPWQKLRSEKQHIKVKKVQNLTDQTSDAAGLESVARSESGTNPREHSPSENEKEVIGADPRGAKPKKAAQFIYSYGRGPKVKGKLKCEWGNRMTPKSEDAVPENTKPVGVIHPDSLDTSFKKSVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGQRHTNATRNNMAPIPKDNLNERPAKSACDNGNLAGVNKSSRRVDPEKCAVRRQDPQVTSFPRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENLLNCGQHHCAELCHGGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLCQLLELGSSTRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQSCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHELRSNIPCHLADISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHPSSPCPMTACKAKIELQCECGRRKEMMICSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECLALERKKRLAEAFHISEDSDPFNVRSSGSKFSDSLKDDARKDLKFVSDIEKEMEALVEAVNKGKNSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSICPPTTLIGVLEREMQSRPPPPIPHHRHQTDKSPVSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_005626706.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626706.3

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCTAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAAATCAGC AAGTTAATTA CTAAGAAAGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAGTGTTTA GCCTTGGAAA GGAAAAAGCG GTTAGCAGAG 
GCATTTCATA TTAGTGAGGA TTCTGATCCT TTCAATGTAC GTTCTTCGGG GTCAAAATTC 
AGTGACAGTT TAAAAGATGA TGCCAGGAAG GATTTAAAGT TTGTCAGTGA CATTGAAAAA 
GAAATGGAAG CCCTTGTGGA GGCCGTGAAT AAGGGAAAGA ATAGTAAGAA GAGCCATTGC 
TTCCCTCCCA TGAACAGAGA CCACCGCCGG ATCATCCACG ACCTGGCCCA AGTGTATGGC 
CTGGAGAGCG TGAGCTATGA CAGTGAACCA AAGCGCAATG TTGTCGTCAC TGCAATCAGG 
GGAAAGTCCA TTTGTCCTCC TACCACGCTG ATTGGTGTCC TTGAAAGGGA AATGCAGTCA 
CGGCCTCCAC CACCAATTCC TCATCACAGA CATCAGACAG ACAAGAGTCC TGTGAGCAGT 
AATTTACAGA AAATAGCCAA GGAACCAGTA ATTGACTACT TTGATGTCCA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_860463.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_865556.2 Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe14498
Clone ID Related Accession (Same CDS sequence)	XM_860463.5 , XM_860463.4
Accession Version	XM_860463.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcriptional repressor NF-X1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860463.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGATGC TACATTTATG 
TGTGATAAGC GGTGTAACAA GAAACGGTTG TGTGGACGGC ATAAATGTAA TGAGATATGC 
TGTGTGGATA AGGAGCACAA GTGCCCTTTG ATTTGTGGGA GGAAACTTCG TTGTGGCCTT 
CATAGATGTG AAGAACCTTG TCATCGTGGG AACTGCCAGT CATGCTGGCA AGCCAGTTTT 
GATGAATTAA CCTGCCACTG TGGTGCATCA GTGATCTACC CTCCAGTTCC CTGTGGTACG 
AGACCCCCCG AGTGTACCCA GACCTGCGCC AGGGTCCATG AATGTGACCA TCCAGTGTAT 
CATTCTTGTC ATAGTGAAGA GAAGTGTCCC CCTTGTACTT TCCTAACTCA GAAATGGTGC 
ATGGGAAAAC ATGAGTTACG AAGCAACATC CCCTGTCACC TGGCTGATAT CTCTTGCGGA 
TTACCCTGCA GTGCCACGCT ACCATGTGGG ATGCACAAGT GTCAGAGACT CTGTCACAAA 
GGAGAGTGTC TTGTGGATGA GCCCTGCAAG CAGCCCTGTA CTACCCCCAG AGCTGACTGT 
GGCCACCCGT GTATGGCGCC CTGCCACCCC AGCTCACCCT GCCCCATGAC TGCTTGTAAA 
GCCAAGATAG AGCTGCAGTG TGAATGTGGA CGAAGAAAGG AGATGATGAT TTGCTCTGAA 
GCATCCAGTA CTTATCAAAG AATAGCTGCT ATTTCCATGG CCTCTAAAAT AACAGACATG 
CAGCTTGGAG ATTCAGTGGA AATCAGCAAG TTAATTACTA AGAAAGAAAT TCACCAAGCC 
AGGCTGGAGT GTGATGAGGA GTGTTTAGCC TTGGAAAGGA AAAAGCGGTT AGCAGAGGCA 
TTTCATATTA GTGAGGATTC TGATCCTTTC AATGTACGTT CTTCGGGGTC AAAATTCAGT 
GACAGTTTAA AAGATGATGC CAGGAAGGAT TTAAAGTTTG TCAGTGACAT TGAAAAAGAA 
ATGGAAGCCC TTGTGGAGGC CGTGAATAAG GGAAAGAATA GTAAGAAGAG CCATTGCTTC 
CCTCCCATGA ACAGAGACCA CCGCCGGATC ATCCACGACC TGGCCCAAGT GTATGGCCTG 
GAGAGCGTGA GCTATGACAG TGAACCAAAG CGCAATGTTG TCGTCACTGC AATCAGGGGA 
AAGTCCATTT GTCCTCCTAC CACGCTGATT GGTGTCCTTG AAAGGGAAAT GCAGTCACGG 
CCTCCACCAC CAATTCCTCA TCACAGACAT CAGACAGACA AGAGTCCTGT GAGCAGTAAT 
TTACAGAAAA TAGCCAAGGA ACCAGTAATT GACTACTTTG ATGTCCAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865556.2
CDS	516..3869
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGSQRRLDSNRIGRRNYSSPPPCHLSRQVPYDDISAVHQHSYPSGSKPKSQQISFQSSASNKSLKSHGLQNQPWQKLRSEKQHIKVKKVQNLTDQTSDAAGLESVARSESGTNPREHSPSENEKEVIGADPRGAKPKKAAQFIYSYGRGPKVKGKLKCEWGNRMTPKSEDAVPENTKPVGVIHPDSLDTSFKKSVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGQRHTNATRNNMAPIPKDNLNERPAKSACDNGNLAGVNKSSRRVDPEKCAVRRQDPQVTSFPRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENLLNCGQHHCAELCHGGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLCQLLELGSSTRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQSCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHELRSNIPCHLADISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHPSSPCPMTACKAKIELQCECGRRKEMMICSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECLALERKKRLAEAFHISEDSDPFNVRSSGSKFSDSLKDDARKDLKFVSDIEKEMEALVEAVNKGKNSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSICPPTTLIGVLEREMQSRPPPPIPHHRHQTDKSPVSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_860463.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860463.5

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGATGC TACATTTATG 
TGTGATAAGC GGTGTAACAA GAAACGGTTG TGTGGACGGC ATAAATGTAA TGAGATATGC 
TGTGTGGATA AGGAGCACAA GTGCCCTTTG ATTTGTGGGA GGAAACTTCG TTGTGGCCTT 
CATAGATGTG AAGAACCTTG TCATCGTGGG AACTGCCAGT CATGCTGGCA AGCCAGTTTT 
GATGAATTAA CCTGCCACTG TGGTGCATCA GTGATCTACC CTCCAGTTCC CTGTGGTACG 
AGACCCCCCG AGTGTACCCA GACCTGCGCC AGGGTCCATG AATGTGACCA TCCAGTGTAT 
CATTCTTGTC ATAGTGAAGA GAAGTGTCCC CCTTGTACTT TCCTAACTCA GAAATGGTGC 
ATGGGAAAAC ATGAGTTACG AAGCAACATC CCCTGTCACC TGGCTGATAT CTCTTGCGGA 
TTACCCTGCA GTGCCACGCT ACCATGTGGG ATGCACAAGT GTCAGAGACT CTGTCACAAA 
GGAGAGTGTC TTGTGGATGA GCCCTGCAAG CAGCCCTGTA CTACCCCCAG AGCTGACTGT 
GGCCACCCGT GTATGGCGCC CTGCCACCCC AGCTCACCCT GCCCCATGAC TGCTTGTAAA 
GCCAAGATAG AGCTGCAGTG TGAATGTGGA CGAAGAAAGG AGATGATGAT TTGCTCTGAA 
GCATCCAGTA CTTATCAAAG AATAGCTGCT ATTTCCATGG CCTCTAAAAT AACAGACATG 
CAGCTTGGAG ATTCAGTGGA AATCAGCAAG TTAATTACTA AGAAAGAAAT TCACCAAGCC 
AGGCTGGAGT GTGATGAGGA GTGTTTAGCC TTGGAAAGGA AAAAGCGGTT AGCAGAGGCA 
TTTCATATTA GTGAGGATTC TGATCCTTTC AATGTACGTT CTTCGGGGTC AAAATTCAGT 
GACAGTTTAA AAGATGATGC CAGGAAGGAT TTAAAGTTTG TCAGTGACAT TGAAAAAGAA 
ATGGAAGCCC TTGTGGAGGC CGTGAATAAG GGAAAGAATA GTAAGAAGAG CCATTGCTTC 
CCTCCCATGA ACAGAGACCA CCGCCGGATC ATCCACGACC TGGCCCAAGT GTATGGCCTG 
GAGAGCGTGA GCTATGACAG TGAACCAAAG CGCAATGTTG TCGTCACTGC AATCAGGGGA 
AAGTCCATTT GTCCTCCTAC CACGCTGATT GGTGTCCTTG AAAGGGAAAT GCAGTCACGG 
CCTCCACCAC CAATTCCTCA TCACAGACAT CAGACAGACA AGAGTCCTGT GAGCAGTAAT 
TTACAGAAAA TAGCCAAGGA ACCAGTAATT GACTACTTTG ATGTCCAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_860463.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_865556.2 Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.

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CloneID	OCe14498
Clone ID Related Accession (Same CDS sequence)	XM_860463.5 , XM_860463.4
Accession Version	XM_860463.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcriptional repressor NF-X1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860463.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGATGC TACATTTATG 
TGTGATAAGC GGTGTAACAA GAAACGGTTG TGTGGACGGC ATAAATGTAA TGAGATATGC 
TGTGTGGATA AGGAGCACAA GTGCCCTTTG ATTTGTGGGA GGAAACTTCG TTGTGGCCTT 
CATAGATGTG AAGAACCTTG TCATCGTGGG AACTGCCAGT CATGCTGGCA AGCCAGTTTT 
GATGAATTAA CCTGCCACTG TGGTGCATCA GTGATCTACC CTCCAGTTCC CTGTGGTACG 
AGACCCCCCG AGTGTACCCA GACCTGCGCC AGGGTCCATG AATGTGACCA TCCAGTGTAT 
CATTCTTGTC ATAGTGAAGA GAAGTGTCCC CCTTGTACTT TCCTAACTCA GAAATGGTGC 
ATGGGAAAAC ATGAGTTACG AAGCAACATC CCCTGTCACC TGGCTGATAT CTCTTGCGGA 
TTACCCTGCA GTGCCACGCT ACCATGTGGG ATGCACAAGT GTCAGAGACT CTGTCACAAA 
GGAGAGTGTC TTGTGGATGA GCCCTGCAAG CAGCCCTGTA CTACCCCCAG AGCTGACTGT 
GGCCACCCGT GTATGGCGCC CTGCCACCCC AGCTCACCCT GCCCCATGAC TGCTTGTAAA 
GCCAAGATAG AGCTGCAGTG TGAATGTGGA CGAAGAAAGG AGATGATGAT TTGCTCTGAA 
GCATCCAGTA CTTATCAAAG AATAGCTGCT ATTTCCATGG CCTCTAAAAT AACAGACATG 
CAGCTTGGAG ATTCAGTGGA AATCAGCAAG TTAATTACTA AGAAAGAAAT TCACCAAGCC 
AGGCTGGAGT GTGATGAGGA GTGTTTAGCC TTGGAAAGGA AAAAGCGGTT AGCAGAGGCA 
TTTCATATTA GTGAGGATTC TGATCCTTTC AATGTACGTT CTTCGGGGTC AAAATTCAGT 
GACAGTTTAA AAGATGATGC CAGGAAGGAT TTAAAGTTTG TCAGTGACAT TGAAAAAGAA 
ATGGAAGCCC TTGTGGAGGC CGTGAATAAG GGAAAGAATA GTAAGAAGAG CCATTGCTTC 
CCTCCCATGA ACAGAGACCA CCGCCGGATC ATCCACGACC TGGCCCAAGT GTATGGCCTG 
GAGAGCGTGA GCTATGACAG TGAACCAAAG CGCAATGTTG TCGTCACTGC AATCAGGGGA 
AAGTCCATTT GTCCTCCTAC CACGCTGATT GGTGTCCTTG AAAGGGAAAT GCAGTCACGG 
CCTCCACCAC CAATTCCTCA TCACAGACAT CAGACAGACA AGAGTCCTGT GAGCAGTAAT 
TTACAGAAAA TAGCCAAGGA ACCAGTAATT GACTACTTTG ATGTCCAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865556.2
CDS	516..3869
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGSQRRLDSNRIGRRNYSSPPPCHLSRQVPYDDISAVHQHSYPSGSKPKSQQISFQSSASNKSLKSHGLQNQPWQKLRSEKQHIKVKKVQNLTDQTSDAAGLESVARSESGTNPREHSPSENEKEVIGADPRGAKPKKAAQFIYSYGRGPKVKGKLKCEWGNRMTPKSEDAVPENTKPVGVIHPDSLDTSFKKSVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGQRHTNATRNNMAPIPKDNLNERPAKSACDNGNLAGVNKSSRRVDPEKCAVRRQDPQVTSFPRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENLLNCGQHHCAELCHGGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLCQLLELGSSTRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQSCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHELRSNIPCHLADISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHPSSPCPMTACKAKIELQCECGRRKEMMICSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECLALERKKRLAEAFHISEDSDPFNVRSSGSKFSDSLKDDARKDLKFVSDIEKEMEALVEAVNKGKNSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSICPPTTLIGVLEREMQSRPPPPIPHHRHQTDKSPVSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_860463.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860463.4

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGATGC TACATTTATG 
TGTGATAAGC GGTGTAACAA GAAACGGTTG TGTGGACGGC ATAAATGTAA TGAGATATGC 
TGTGTGGATA AGGAGCACAA GTGCCCTTTG ATTTGTGGGA GGAAACTTCG TTGTGGCCTT 
CATAGATGTG AAGAACCTTG TCATCGTGGG AACTGCCAGT CATGCTGGCA AGCCAGTTTT 
GATGAATTAA CCTGCCACTG TGGTGCATCA GTGATCTACC CTCCAGTTCC CTGTGGTACG 
AGACCCCCCG AGTGTACCCA GACCTGCGCC AGGGTCCATG AATGTGACCA TCCAGTGTAT 
CATTCTTGTC ATAGTGAAGA GAAGTGTCCC CCTTGTACTT TCCTAACTCA GAAATGGTGC 
ATGGGAAAAC ATGAGTTACG AAGCAACATC CCCTGTCACC TGGCTGATAT CTCTTGCGGA 
TTACCCTGCA GTGCCACGCT ACCATGTGGG ATGCACAAGT GTCAGAGACT CTGTCACAAA 
GGAGAGTGTC TTGTGGATGA GCCCTGCAAG CAGCCCTGTA CTACCCCCAG AGCTGACTGT 
GGCCACCCGT GTATGGCGCC CTGCCACCCC AGCTCACCCT GCCCCATGAC TGCTTGTAAA 
GCCAAGATAG AGCTGCAGTG TGAATGTGGA CGAAGAAAGG AGATGATGAT TTGCTCTGAA 
GCATCCAGTA CTTATCAAAG AATAGCTGCT ATTTCCATGG CCTCTAAAAT AACAGACATG 
CAGCTTGGAG ATTCAGTGGA AATCAGCAAG TTAATTACTA AGAAAGAAAT TCACCAAGCC 
AGGCTGGAGT GTGATGAGGA GTGTTTAGCC TTGGAAAGGA AAAAGCGGTT AGCAGAGGCA 
TTTCATATTA GTGAGGATTC TGATCCTTTC AATGTACGTT CTTCGGGGTC AAAATTCAGT 
GACAGTTTAA AAGATGATGC CAGGAAGGAT TTAAAGTTTG TCAGTGACAT TGAAAAAGAA 
ATGGAAGCCC TTGTGGAGGC CGTGAATAAG GGAAAGAATA GTAAGAAGAG CCATTGCTTC 
CCTCCCATGA ACAGAGACCA CCGCCGGATC ATCCACGACC TGGCCCAAGT GTATGGCCTG 
GAGAGCGTGA GCTATGACAG TGAACCAAAG CGCAATGTTG TCGTCACTGC AATCAGGGGA 
AAGTCCATTT GTCCTCCTAC CACGCTGATT GGTGTCCTTG AAAGGGAAAT GCAGTCACGG 
CCTCCACCAC CAATTCCTCA TCACAGACAT CAGACAGACA AGAGTCCTGT GAGCAGTAAT 
TTACAGAAAA TAGCCAAGGA ACCAGTAATT GACTACTTTG ATGTCCAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005626707.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626764.1 Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe14499
Clone ID Related Accession (Same CDS sequence)	XM_005626707.2 , XM_005626707.3
Accession Version	XM_005626707.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcriptional repressor NF-X1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCTAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAAATCAGC AAGTTAATTA CTAAGAAAGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAGTGTTTA GCCTTGGAAA GGAAAAAGCG GTTAGCAGAG 
GCATTTCATA TTAGTGAGGA TTCTGATCCT TTCAATGTAC GTTCTTCGGG GTCAAAATTC 
AGTGACAGTT TAAAAGATGA TGCCAGGAAG GATTTAAAGT TTGTCAGTGA CATTGAAAAA 
GAAATGGAAG CCCTTGTGGA GGCCGTGAAT AAGGTTGAAG TCAAAACATC CAACTGGACG 
TGTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626764.1
CDS	516..3584
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGSQRRLDSNRIGRRNYSSPPPCHLSRQVPYDDISAVHQHSYPSGSKPKSQQISFQSSASNKSLKSHGLQNQPWQKLRSEKQHIKVKKVQNLTDQTSDAAGLESVARSESGTNPREHSPSENEKEVIGADPRGAKPKKAAQFIYSYGRGPKVKGKLKCEWGNRMTPKSEDAVPENTKPVGVIHPDSLDTSFKKSVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGQRHTNATRNNMAPIPKDNLNERPAKSACDNGNLAGVNKSSRRVDPEKCAVRRQDPQVTSFPRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENLLNCGQHHCAELCHGGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLCQLLELGSSTRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQSCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHELRSNIPCHLADISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHPSSPCPMTACKAKIELQCECGRRKEMMICSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECLALERKKRLAEAFHISEDSDPFNVRSSGSKFSDSLKDDARKDLKFVSDIEKEMEALVEAVNKVEVKTSNWTCL

Target ORF information:

RefSeq Version	XM_005626707.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626707.2

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCTAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAAATCAGC AAGTTAATTA CTAAGAAAGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAGTGTTTA GCCTTGGAAA GGAAAAAGCG GTTAGCAGAG 
GCATTTCATA TTAGTGAGGA TTCTGATCCT TTCAATGTAC GTTCTTCGGG GTCAAAATTC 
AGTGACAGTT TAAAAGATGA TGCCAGGAAG GATTTAAAGT TTGTCAGTGA CATTGAAAAA 
GAAATGGAAG CCCTTGTGGA GGCCGTGAAT AAGGTTGAAG TCAAAACATC CAACTGGACG 
TGTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005626707.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626764.1 Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe14499
Clone ID Related Accession (Same CDS sequence)	XM_005626707.2 , XM_005626707.3
Accession Version	XM_005626707.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcriptional repressor NF-X1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCTAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAAATCAGC AAGTTAATTA CTAAGAAAGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAGTGTTTA GCCTTGGAAA GGAAAAAGCG GTTAGCAGAG 
GCATTTCATA TTAGTGAGGA TTCTGATCCT TTCAATGTAC GTTCTTCGGG GTCAAAATTC 
AGTGACAGTT TAAAAGATGA TGCCAGGAAG GATTTAAAGT TTGTCAGTGA CATTGAAAAA 
GAAATGGAAG CCCTTGTGGA GGCCGTGAAT AAGGTTGAAG TCAAAACATC CAACTGGACG 
TGTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626764.1
CDS	516..3584
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGSQRRLDSNRIGRRNYSSPPPCHLSRQVPYDDISAVHQHSYPSGSKPKSQQISFQSSASNKSLKSHGLQNQPWQKLRSEKQHIKVKKVQNLTDQTSDAAGLESVARSESGTNPREHSPSENEKEVIGADPRGAKPKKAAQFIYSYGRGPKVKGKLKCEWGNRMTPKSEDAVPENTKPVGVIHPDSLDTSFKKSVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGQRHTNATRNNMAPIPKDNLNERPAKSACDNGNLAGVNKSSRRVDPEKCAVRRQDPQVTSFPRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENLLNCGQHHCAELCHGGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLCQLLELGSSTRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQSCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHELRSNIPCHLADISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHPSSPCPMTACKAKIELQCECGRRKEMMICSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECLALERKKRLAEAFHISEDSDPFNVRSSGSKFSDSLKDDARKDLKFVSDIEKEMEALVEAVNKVEVKTSNWTCL

Target ORF information:

RefSeq Version	XM_005626707.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626707.3

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGAA GTCAAAGGAG ACTAGATTCT 
AATAGGATTG GGAGAAGAAA TTATAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAAGTC 
CCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTATC CTTCAGGAAG CAAACCTAAG 
AGTCAACAGA TTTCTTTCCA ATCCTCTGCT TCTAACAAAT CACTAAAGAG CCATGGCCTT 
CAGAATCAAC CTTGGCAGAA ATTGAGAAGT GAAAAGCAGC ACATCAAAGT CAAAAAAGTA 
CAGAATCTCA CTGATCAGAC CTCAGATGCA GCTGGCTTAG AAAGTGTGGC TAGGTCAGAG 
AGTGGGACAA ACCCCAGAGA GCACAGCCCT TCTGAGAATG AGAAGGAAGT TATTGGTGCA 
GATCCCAGGG GAGCAAAACC CAAAAAAGCA GCCCAATTTA TATACAGTTA TGGTAGAGGA 
CCAAAAGTCA AGGGAAAACT CAAATGTGAG TGGGGTAACA GAATGACTCC AAAATCCGAG 
GATGCTGTAC CTGAAAATAC CAAACCTGTG GGGGTTATCC ACCCTGACTC TTTGGATACA 
TCCTTTAAGA AAAGTGTATT GGATGGGTAT GGGGCCAGAC GCAATGAGCA GAGAAGATAC 
CCACAGAAAA GGCCTCCTTG GGAAGTGGAA GGAGCCAGGC CACGACCAGG CAGGAATCCA 
CCAAAACAAG AGGGCCAACG ACATACAAAT GCCACCAGAA ACAACATGGC CCCCATTCCA 
AAGGATAATC TTAATGAGAG ACCCGCAAAA TCTGCCTGTG ACAATGGGAA CTTGGCAGGT 
GTCAACAAGT CTTCCCGAAG GGTTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCT 
CAAGTAACGT CTTTTCCCCG AGGCAAACAG AACCATGTGC TAAAGAATGT GGAAACACAC 
ACAGGTTCTC TAATTGAACA GCTAACTACA GAAAAATATG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGCG TCACAGCACC AGTGTGGAGT TGTCAGAGCT GTTATCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC AAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGATGCCCTG CCTGTCAGAA TGTTTCTGCA CACGTTCCTA ATACTTACAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTT 
TGTAGAAAGA AACAGCCTGG CCAGGACTGT CCACATTCCT GTAACCTTCT CTGTCATCCT 
GGACCCTGCC CGCCCTGCCC TGCCTTTATG ACTAAAACGT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGTGGTCA GGCTGTCTCA GTCCACTGTT CCAATCCATG TGAGAATCTT 
CTGAATTGTG GTCAGCACCA CTGTGCCGAG CTATGCCATG GGGGTCAATG CCAGCCTTGT 
CGGATCATAT TGAACCAGGT ATGCTACTGT GGCAGCACTT CCCGAGATGT GTTATGTGGA 
ACAGATGTAG GAAAGTCTGA TGGATTTGGC GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACATGTTCT CAAGTATGCC ACCCTCAGCC CTGCCAGCCA 
TGCCCACGGC TCCCCCAGCT GGTGCGCTAT TGCCCTTGTG GCCAAACTCC TCTCTGCCAA 
TTGCTAGAAC TTGGAAGTAG TACTCGGAAA ACATGCATGG ATCCTGTCCC TTCATGCGGA 
AAAGTGTGCG GCAAGCCTCT GCCATGTGGT TCTTTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGCCATG AAGGAGACTG TGGACCATGC TCTCGTACAT CGGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCGTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGTGATA AGCGGTGTAA CAAGAAACGG TTGTGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGCCCT TTGATTTGTG GGAGGAAACT TCGTTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGGAACTGCC AGTCATGCTG GCAAGCCAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGT 
ACGAGACCCC CCGAGTGTAC CCAGACCTGC GCCAGGGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCTT GTCATAGTGA AGAGAAGTGT CCCCCTTGTA CTTTCCTAAC TCAGAAATGG 
TGCATGGGAA AACATGAGTT ACGAAGCAAC ATCCCCTGTC ACCTGGCTGA TATCTCTTGC 
GGATTACCCT GCAGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG ACTCTGTCAC 
AAAGGAGAGT GTCTTGTGGA TGAGCCCTGC AAGCAGCCCT GTACTACCCC CAGAGCTGAC 
TGTGGCCACC CGTGTATGGC GCCCTGCCAC CCCAGCTCAC CCTGCCCCAT GACTGCTTGT 
AAAGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AGGAGATGAT GATTTGCTCT 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCTAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAAATCAGC AAGTTAATTA CTAAGAAAGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAGTGTTTA GCCTTGGAAA GGAAAAAGCG GTTAGCAGAG 
GCATTTCATA TTAGTGAGGA TTCTGATCCT TTCAATGTAC GTTCTTCGGG GTCAAAATTC 
AGTGACAGTT TAAAAGATGA TGCCAGGAAG GATTTAAAGT TTGTCAGTGA CATTGAAAAA 
GAAATGGAAG CCCTTGTGGA GGCCGTGAAT AAGGTTGAAG TCAAAACATC CAACTGGACG 
TGTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NFX1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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