pikfyve gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009304723.3	XP_009302998.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_009304724.3	XP_009302999.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_005167667.4	XP_005167724.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
XM_009304726.3	XP_009303001.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
XM_005167666.3	XP_005167723.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_005167667.3	XP_005167724.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
XM_005167669.3	XP_005167726.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
XM_009304726.2	XP_009303001.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
XM_009304721.3	XP_009302996.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_005167669.4	XP_005167726.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
NM_001127305.1	NP_001120777.1	1-phosphatidylinositol 3-phosphate 5-kinase
XM_009304722.2	XP_009302997.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_005167670.3	XP_005167727.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
XM_009304724.2	XP_009302999.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_009304725.2	XP_009303000.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_009304725.3	XP_009303000.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_009304721.2	XP_009302996.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_009304723.2	XP_009302998.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_005167665.3	XP_005167722.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_009304722.3	XP_009302997.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_005167665.4	XP_005167722.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_005167666.4	XP_005167723.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_005167670.4	XP_005167727.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40127	XM_009304726.3 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa40122	XM_009304721.3 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40123	XM_009304722.3 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40124	XM_009304723.3 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40125	XM_009304724.3 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40126	XM_009304725.3 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa15216	NM_001127305.1 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa15215	XM_005167665.4 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa15216	XM_005167666.4 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa15217	XM_005167667.4 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa15219	XM_005167669.4 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa15220	XM_005167670.4 Latest version!	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa15217	XM_005167667.3	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa40127	XM_009304726.2	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa15219	XM_005167669.3	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa40125	XM_009304724.2	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40126	XM_009304725.2	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40122	XM_009304721.2	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40124	XM_009304723.2	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa40123	XM_009304722.2	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa15215	XM_005167665.3	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa15216	XM_005167666.3	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa15220	XM_005167670.3	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

pikfyve ( XM_009304726.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303001.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa40127
Clone ID Related Accession (Same CDS sequence)	XM_009304726.3 , XM_009304726.2
Accession Version	XM_009304726.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACATC ATTCCTTTTA TTTTTTTGTT GCATTCATGA GGGAATTTCC GTCTGACAAC 
AAATTACATC ATCAGGAATC TCAGAACAGC GGTCTCAATT CCAGACTGAC AGTGGTCCAA 
GAGGATATTG GAAAGTCACC TGCCAGGAAG AGGTCAGCTA GTGTGACCAA TTTGTCTTTG 
GACCGTTCCG GCTCATCCAT GGTTCCTGCC TATGAGAGTT CGGTTAGTCC ACAGAACAGC 
CGAGCTCTAC CCAAGACCGA CCACAGCGAA GAAGAGCGAA AGATCCTTCT AGATTCTTTT 
CAACTTAAAG ATATATGGAA GAAGATTTGC CACAACACTA CTGGGATGGA GTTCCAGGAC 
CATCGGTACT GGCTGCGCAC TTACCCCAAC TGCATTGTGG GTAAAGAGTT GGTCAACTGG 
CTTTTAAGAA ATGGCACTAT TTCCACTAGG GCTCAAGCCA TAGCTATAGG ACAGGCTTTG 
GTAGATGGCC GCTGGCTTGA CTGTGTAACC CATCATGATC AGATCTTCCG TGATGAGTAC 
GCCCTTTATC GCCCCCTTCA GAGCACAGAG TTCTCCGAAA CTCCTTCTCC AGACAGTGAC 
AGTGTTAATT CCCTGGAGGG ACACTCTGAA CCGTCATGGT TTAAAGACAT CAAGTTTGAT 
GACAGTGACA CCGAGCAGCT AGCAGATGAA AATGACTATG TCACACCAAG CTCGTCCAGC 
CCCAGCAAAA GAACATCTGT CAGCAGTTTC CACTCTGCAG TAGACAGTGA CTCGGCCGCC 
TCCATCAGTC TAAACATGGA GCAGGACAAC GTCAATTTCC ACATTAAGAA GCAGGCCAAG 
TACCCCCATG TGCCTCCCTA CCCAGCTGAG CAAAAAAGTA TGGAGCCCCA TGACCCGTTC 
ACCCCAGAAA CCGACATCCA TGCACCAATG GAAGTCTTGC TCTCCGAAGA TGGAGGTCAG 
CATATATCCA TCAGTGATGC ATTCATCAAA GAGTCTTTGT TCAACCGGAG GGTTGAGGAG 
AAAGCTAAAG AGATGCTCTT CACTCCTCTC GGCTGGCATC ACAGCTCTCT GGATCAGCTG 
CGGGAAGAGA ATGGGGAAAA GAAAGCGATG GAGCGGTTGC TGTCAGCAAA TCACAGCCAC 
ATGATGGCGC TGCTGCAGCA ACTGCTGTAC AGTGAATCAC TGTCTCTGTC GTGGCGTGAC 
ATCATCGTAC CTGTGGTGAG GCAGGTGGTG CAGACGGTGC GACCGGATGT GCGCAGCTGT 
GACGATGATA TGGACATCCG TCAGTTTGTC CACATCAAGA AGATTCCAGG AGGAAAGAAG 
TTTGACTCTG CTGTAGTGAA TGGTTTTGTT TGCACAAAGA ATATCGCACA CAAAAAGATG 
AACTCTTACA TCAAAAACCC CAAGATCCTT CTTCTCAAAT GCTCCATTGA GTATCTATAT 
AGAGAGGAGA CCAAGTTCAC CTGCATTGAC CCAATTGTTT TACAGGAGCG TGAGTTTCTT 
AAGAACTATG TTCAGAGGAT TGTTGATGTC CGACCAAACT TAGTCCTGGT GGAGAAGACT 
GTATCGCGAA TTGCTCAGGA CATGCTACTA GAGCACGGCA TTGCCATTGT GATTAATGTC 
AAACCTCAAG TACTGGACCG TGTGAGTCGA ATGACTCAGG GAGATTTAGT CATTTCAATG 
GATCAGCTTC TTACAAAACC TCGTCTGGGT ACCTGCCACA AGTTTTACTT GCACTCCTTC 
CAGCTACCAA ATAGTGAAGT GAAAACCCTG ATGTTTTTTG AGGGCTGTCC TCCTCAGCTG 
GGCTGCACTA TTAAGCTCCG CGGAGCCTCA GAGTATGAGC TGGCACGGGT AAAAGAGATC 
ATCATTTTTA TGGTGTGTGT GGCATACCAC TCGCAGCTAG AGATCTCTTT CCTCATGGAT 
GAGTTCGCTA TGCCTCCCAG TCTGGCTGAG AGTTCTTCTT TCCCATGTCT GCTGGAAAGC 
ACCACTTTAC GGGAAGAGGA TGAAACTAGT GGGGAACGGG AAAGCCAAGA CAATGATGAG 
TCTACACTGG GAGATGACAA TCTCACACTT GTTCCAGGAG ATGACTTTGA GACAGGATTT 
ACAGGGACTA TCAAAGCCCA CAGTCGAGAG CCTTCCACCT CTGAATCTTC TCATAAAGAT 
GGAGAAAGCC CCAGAATAAA TAAAAATGCT TCAATTACCT CCTTCTCTGG AGGGGAGGAA 
GAAAATATAA AGACCTCCAC GCCACTTTCC TCCTTCTCTT CCACCTTTCC TCAGCCGGTG 
ACACCACCTT TCCTGATATC AGACGTAAAA GAAATGTCAC AGGAAGTGGT CAAGGGGTCA 
GGTGAAGGGG AGAAGAACAA ATATCTGGAG TCGACTCTGG TCCATCAGGA CAGCACAAGC 
TCTGAGACCT CCCTTCCCCC AACTCGGCTC TTTAGAGACC CCTTACAGGA CGACACAGGC 
CTGTTTGTGA CAGAGCATGT AGCTTCTTCA GATGACCGCC TCAAATCCAT CTCTGCTTTA 
TTCAAACAGG AGCTAAAAGA CATCATCCTG TGCATTTCAC CCTTTATTAC CTTTCGAGAG 
CCTTTCTTGC TTACCCCAGC TGGACTGCGG TGTCCTAGCC GGGATTATTT CCCTGAACAG 
GTTTACCTTT CACCTCTGCT GAACAAGGAT TCAAAAGAAC TGGATGGACG CCGCAAGAGG 
CAGCTGCTGA AAGAGTCCAG CCCAGGTTCT GGTAGCCTGA CCAATGGTTC CGTTTCACAC 
CAGAGAGCCA TTCAGATCTT GCCCTGTCAC AAACTCACTA GTGCCCGAAT AGCAGAACAG 
GTAGGCAGCA GTCAGCAGCT GGCACGCATG TTAGCGGACT ACCGTGCCCA AGGAGGACGT 
ATCCGGCAAA GAGAGGGAAC ACAATTTCGT GAAGCCCCTC CCCCAAAGGC GCCAATAAAG 
GCAGATAGTG AAGAAGATAA AGGTGCAGGA GTGATTGACA TGACCTGGTC TACTAAGTTG 
GACTGTTTAA ATCCAATCAA CCATCAGAGG CTCTGTGTTC TGTTCAGTAG CTCTTCAGCA 
CAGTCTAATA ATGCCCCAAA CCCCTGCGTC AGTCCCTGGA TTGTTACAAT GGAGTTTTAT 
GGAAAGAATG ACTTGACTCT TGGTGTGTTT CTGGAGAGAT ACTGTTTTAG ACCATCTTAC 
CAGTGCCCCA GCATGTACTG TGAGACCCCC ATGGTTCACC ACATCCGGCG CTTTGTGCAC 
GGTAAAGGCT GCGTCCAGAT TGTACTGAAA GAGCTGGACT CGCCTGTTCC TGGATATCAG 
CACACAATCC TCAACTACTC ATGGTGTCGC ATCTGCAAAC AGGTGACTCC TGTGGTCCCT 
TTGTCTAATG ACTCTTGGTC CATGTCCTTC GCCAAGTACC TTGAGCTTCG TTTCTATGGT 
CATCAGTACA CCCGTCGGGC TAACGCTGAG CCTTGTGGCC ACTCTATCCA TAAAGATTAT 
CACCAGTACT TCTCTTACAA CCAGATGGTG GCATCCTTCA GCTACATTCC AGTGAGGCTG 
TTAGAGATCT GTCTGCCTCC CCCGAAGATC ATCATCAGGA ACCAAGGTCC ATCGAAATCC 
AGCTTGCAGC AGGATCTCAA AGACTTCTCC CAGAAGGTGG CTCAGGTGTA CCTGGCTATA 
GATGACCGTC TTACCTCCTT AAAAACGGAC ACATTCAGCA AGACCAGAGA GGAAAAAATG 
GAGGACATGT TTGCACAGAA AGATATGGAG GAATCAGAGC TTCGGGGCTG GATAGAGAAG 
TTACAAGTGC GTCTGCAGAC GAGTGTGATT GACTCTTCAC AACAACTACA GGCTGTTCTG 
GAGTCAGTGG TGGTCAAAAA ACAGGGCTTG TGTGAAACCC TGCAGTCCTG GAACAACAGA 
CTTCAAGAAC TGTTCCAGCA AGAGAAAGGC AGAAAACGTC TATCTGTTCC TCCTAGCCCA 
GGCAGACACA GACAACCTAC AACAGATGAG AGCAAGACTA GTGCTTTGGA GTCTTCTCCT 
CGTAACCCTT CCCCAGTGGT CCCAAATGGA GACAAAGAGG ACCGTCATCT CAATACATTT 
CCATCAAGTT CAGGGTCCTC GTCGTTACTA CAGTTACCAT CTCCAGCTGA ACAACCTTCA 
GATGTCATTA CAAGCGGACC ATCTTTCCCT GATCAGGACT CTGTTAGCAA TCCAGAGGAC 
ATGTTTGATG GACACTTGCT TGGTTCCAAT GATAGTCAGG TAAAGGAAAA GTCCACCATG 
AAAACAATCC TGGCCAACCT GTTACCTGGA AACAGCTACA ATCCCATCCC TTTTCCTTTT 
GACCCAGACA AGCACTATCT GATGTATGAG CATGAGAGAG TTCCTATAGC CGTGTGTGAG 
AGAGAGCCCA GCTCCATAAT TGCCTTTGCA CTCAGCTGCA AAGAGTATAA AACTGCCCTT 
GAAGAGCTCA CAAAGACAAC AGCAAAGACT GGAGGTGATG ACACTTCTCA GACCATCAGT 
GCTGGAGAGA GCAGAGTGAA GAACAGTCCA GCCAAACCCA GTGACAACAG CATGTCACAG 
CTGAGCCGCA GCAGCGTCGA CGCTGATCCA CTCAAGGAGC CTGAAAGTGC AGACAAACAG 
AAGAAGCAGA CAGGGAACCC ACATATTGAG CTGCAGTTCT CCGATGCAAA TGCTAAGTTC 
TACTGCCGGA TCTACTATGC TGAAGAGTTC CATAAAATGC GGGAGGAGAT CATGGAGAGC 
ACAGAGGATG ATTTTGTGCG ATCTCTTTCC CACTGTGTCA ACTGGCAGGC GCGTGGTGGG 
AAATCTGGTG CAGTTTTCTA TGCCACTGAA GATGATCGGT TTATTCTGAA GCAGATGCCC 
AGATTAGAGG TCCAGTCCTT TTTAGACTTT GCACCCCACT ACTTTACGTA CATCACAGGA 
GCGGTTCACC TAAAGCGACC CACAGCACTT GCTAAGATTC TGGGAGTGTA CCGCATCGGC 
TACAAGAACT CCCAGAACAA CACTGAGAAG AAGCTTGACC TGCTTGTGAT GGAAAACCTT 
TTCTATGGAC GAAAGATGGC ACAGGTGTTT GACCTGAAAG GATCGCTGAG AAACAGGAAC 
GTGAAGACGG ACCAGGGAAA GGAGAGCTGT GAGGTGGTGC TCCTAGATGA GAACCTGCTC 
AAACTGGTGC ATGACAATCC CCTTTATATT CGCTCGCACT GCAAGGCCAT CCTGCGTGCT 
GCCATCCACA GCGACGCGCT CTTCCTGTCC AGCCACCTCA TCATTGATTA CTCCCTGCTG 
GTGGGCCGAG ACGATTCCAC AGATGAGCTA GTTGTGGGGA TCATAGATTA TATCCGGACT 
TTCACTTGGG ATAAAAAGCT GGAGATGGTG GTCAAATCTA CTGGGATTCT TGGAGGTCAA 
GGTAAAATGC CCACAGTGGT GTCACCAGAG CTGTATCGAA CACGTTTCTG TGAGGCCATG 
GATAAATACT TCCTTATGGT CCCTGATCAC TGGAGTGGAC TTGGGCTCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303001.1
CDS	511..6027
Translation	MVHHSFYFFVAFMREFPSDNKLHHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304726.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304726.3

ORF Insert Sequence:

ATGGTACATC ATTCCTTTTA TTTTTTTGTT GCATTCATGA GGGAATTTCC GTCTGACAAC 
AAATTACATC ATCAGGAATC TCAGAACAGC GGTCTCAATT CCAGACTGAC AGTGGTCCAA 
GAGGATATTG GAAAGTCACC TGCCAGGAAG AGGTCAGCTA GTGTGACCAA TTTGTCTTTG 
GACCGTTCCG GCTCATCCAT GGTTCCTGCC TATGAGAGTT CGGTTAGTCC ACAGAACAGC 
CGAGCTCTAC CCAAGACCGA CCACAGCGAA GAAGAGCGAA AGATCCTTCT AGATTCTTTT 
CAACTTAAAG ATATATGGAA GAAGATTTGC CACAACACTA CTGGGATGGA GTTCCAGGAC 
CATCGGTACT GGCTGCGCAC TTACCCCAAC TGCATTGTGG GTAAAGAGTT GGTCAACTGG 
CTTTTAAGAA ATGGCACTAT TTCCACTAGG GCTCAAGCCA TAGCTATAGG ACAGGCTTTG 
GTAGATGGCC GCTGGCTTGA CTGTGTAACC CATCATGATC AGATCTTCCG TGATGAGTAC 
GCCCTTTATC GCCCCCTTCA GAGCACAGAG TTCTCCGAAA CTCCTTCTCC AGACAGTGAC 
AGTGTTAATT CCCTGGAGGG ACACTCTGAA CCGTCATGGT TTAAAGACAT CAAGTTTGAT 
GACAGTGACA CCGAGCAGCT AGCAGATGAA AATGACTATG TCACACCAAG CTCGTCCAGC 
CCCAGCAAAA GAACATCTGT CAGCAGTTTC CACTCTGCAG TAGACAGTGA CTCGGCCGCC 
TCCATCAGTC TAAACATGGA GCAGGACAAC GTCAATTTCC ACATTAAGAA GCAGGCCAAG 
TACCCCCATG TGCCTCCCTA CCCAGCTGAG CAAAAAAGTA TGGAGCCCCA TGACCCGTTC 
ACCCCAGAAA CCGACATCCA TGCACCAATG GAAGTCTTGC TCTCCGAAGA TGGAGGTCAG 
CATATATCCA TCAGTGATGC ATTCATCAAA GAGTCTTTGT TCAACCGGAG GGTTGAGGAG 
AAAGCTAAAG AGATGCTCTT CACTCCTCTC GGCTGGCATC ACAGCTCTCT GGATCAGCTG 
CGGGAAGAGA ATGGGGAAAA GAAAGCGATG GAGCGGTTGC TGTCAGCAAA TCACAGCCAC 
ATGATGGCGC TGCTGCAGCA ACTGCTGTAC AGTGAATCAC TGTCTCTGTC GTGGCGTGAC 
ATCATCGTAC CTGTGGTGAG GCAGGTGGTG CAGACGGTGC GACCGGATGT GCGCAGCTGT 
GACGATGATA TGGACATCCG TCAGTTTGTC CACATCAAGA AGATTCCAGG AGGAAAGAAG 
TTTGACTCTG CTGTAGTGAA TGGTTTTGTT TGCACAAAGA ATATCGCACA CAAAAAGATG 
AACTCTTACA TCAAAAACCC CAAGATCCTT CTTCTCAAAT GCTCCATTGA GTATCTATAT 
AGAGAGGAGA CCAAGTTCAC CTGCATTGAC CCAATTGTTT TACAGGAGCG TGAGTTTCTT 
AAGAACTATG TTCAGAGGAT TGTTGATGTC CGACCAAACT TAGTCCTGGT GGAGAAGACT 
GTATCGCGAA TTGCTCAGGA CATGCTACTA GAGCACGGCA TTGCCATTGT GATTAATGTC 
AAACCTCAAG TACTGGACCG TGTGAGTCGA ATGACTCAGG GAGATTTAGT CATTTCAATG 
GATCAGCTTC TTACAAAACC TCGTCTGGGT ACCTGCCACA AGTTTTACTT GCACTCCTTC 
CAGCTACCAA ATAGTGAAGT GAAAACCCTG ATGTTTTTTG AGGGCTGTCC TCCTCAGCTG 
GGCTGCACTA TTAAGCTCCG CGGAGCCTCA GAGTATGAGC TGGCACGGGT AAAAGAGATC 
ATCATTTTTA TGGTGTGTGT GGCATACCAC TCGCAGCTAG AGATCTCTTT CCTCATGGAT 
GAGTTCGCTA TGCCTCCCAG TCTGGCTGAG AGTTCTTCTT TCCCATGTCT GCTGGAAAGC 
ACCACTTTAC GGGAAGAGGA TGAAACTAGT GGGGAACGGG AAAGCCAAGA CAATGATGAG 
TCTACACTGG GAGATGACAA TCTCACACTT GTTCCAGGAG ATGACTTTGA GACAGGATTT 
ACAGGGACTA TCAAAGCCCA CAGTCGAGAG CCTTCCACCT CTGAATCTTC TCATAAAGAT 
GGAGAAAGCC CCAGAATAAA TAAAAATGCT TCAATTACCT CCTTCTCTGG AGGGGAGGAA 
GAAAATATAA AGACCTCCAC GCCACTTTCC TCCTTCTCTT CCACCTTTCC TCAGCCGGTG 
ACACCACCTT TCCTGATATC AGACGTAAAA GAAATGTCAC AGGAAGTGGT CAAGGGGTCA 
GGTGAAGGGG AGAAGAACAA ATATCTGGAG TCGACTCTGG TCCATCAGGA CAGCACAAGC 
TCTGAGACCT CCCTTCCCCC AACTCGGCTC TTTAGAGACC CCTTACAGGA CGACACAGGC 
CTGTTTGTGA CAGAGCATGT AGCTTCTTCA GATGACCGCC TCAAATCCAT CTCTGCTTTA 
TTCAAACAGG AGCTAAAAGA CATCATCCTG TGCATTTCAC CCTTTATTAC CTTTCGAGAG 
CCTTTCTTGC TTACCCCAGC TGGACTGCGG TGTCCTAGCC GGGATTATTT CCCTGAACAG 
GTTTACCTTT CACCTCTGCT GAACAAGGAT TCAAAAGAAC TGGATGGACG CCGCAAGAGG 
CAGCTGCTGA AAGAGTCCAG CCCAGGTTCT GGTAGCCTGA CCAATGGTTC CGTTTCACAC 
CAGAGAGCCA TTCAGATCTT GCCCTGTCAC AAACTCACTA GTGCCCGAAT AGCAGAACAG 
GTAGGCAGCA GTCAGCAGCT GGCACGCATG TTAGCGGACT ACCGTGCCCA AGGAGGACGT 
ATCCGGCAAA GAGAGGGAAC ACAATTTCGT GAAGCCCCTC CCCCAAAGGC GCCAATAAAG 
GCAGATAGTG AAGAAGATAA AGGTGCAGGA GTGATTGACA TGACCTGGTC TACTAAGTTG 
GACTGTTTAA ATCCAATCAA CCATCAGAGG CTCTGTGTTC TGTTCAGTAG CTCTTCAGCA 
CAGTCTAATA ATGCCCCAAA CCCCTGCGTC AGTCCCTGGA TTGTTACAAT GGAGTTTTAT 
GGAAAGAATG ACTTGACTCT TGGTGTGTTT CTGGAGAGAT ACTGTTTTAG ACCATCTTAC 
CAGTGCCCCA GCATGTACTG TGAGACCCCC ATGGTTCACC ACATCCGGCG CTTTGTGCAC 
GGTAAAGGCT GCGTCCAGAT TGTACTGAAA GAGCTGGACT CGCCTGTTCC TGGATATCAG 
CACACAATCC TCAACTACTC ATGGTGTCGC ATCTGCAAAC AGGTGACTCC TGTGGTCCCT 
TTGTCTAATG ACTCTTGGTC CATGTCCTTC GCCAAGTACC TTGAGCTTCG TTTCTATGGT 
CATCAGTACA CCCGTCGGGC TAACGCTGAG CCTTGTGGCC ACTCTATCCA TAAAGATTAT 
CACCAGTACT TCTCTTACAA CCAGATGGTG GCATCCTTCA GCTACATTCC AGTGAGGCTG 
TTAGAGATCT GTCTGCCTCC CCCGAAGATC ATCATCAGGA ACCAAGGTCC ATCGAAATCC 
AGCTTGCAGC AGGATCTCAA AGACTTCTCC CAGAAGGTGG CTCAGGTGTA CCTGGCTATA 
GATGACCGTC TTACCTCCTT AAAAACGGAC ACATTCAGCA AGACCAGAGA GGAAAAAATG 
GAGGACATGT TTGCACAGAA AGATATGGAG GAATCAGAGC TTCGGGGCTG GATAGAGAAG 
TTACAAGTGC GTCTGCAGAC GAGTGTGATT GACTCTTCAC AACAACTACA GGCTGTTCTG 
GAGTCAGTGG TGGTCAAAAA ACAGGGCTTG TGTGAAACCC TGCAGTCCTG GAACAACAGA 
CTTCAAGAAC TGTTCCAGCA AGAGAAAGGC AGAAAACGTC TATCTGTTCC TCCTAGCCCA 
GGCAGACACA GACAACCTAC AACAGATGAG AGCAAGACTA GTGCTTTGGA GTCTTCTCCT 
CGTAACCCTT CCCCAGTGGT CCCAAATGGA GACAAAGAGG ACCGTCATCT CAATACATTT 
CCATCAAGTT CAGGGTCCTC GTCGTTACTA CAGTTACCAT CTCCAGCTGA ACAACCTTCA 
GATGTCATTA CAAGCGGACC ATCTTTCCCT GATCAGGACT CTGTTAGCAA TCCAGAGGAC 
ATGTTTGATG GACACTTGCT TGGTTCCAAT GATAGTCAGG TAAAGGAAAA GTCCACCATG 
AAAACAATCC TGGCCAACCT GTTACCTGGA AACAGCTACA ATCCCATCCC TTTTCCTTTT 
GACCCAGACA AGCACTATCT GATGTATGAG CATGAGAGAG TTCCTATAGC CGTGTGTGAG 
AGAGAGCCCA GCTCCATAAT TGCCTTTGCA CTCAGCTGCA AAGAGTATAA AACTGCCCTT 
GAAGAGCTCA CAAAGACAAC AGCAAAGACT GGAGGTGATG ACACTTCTCA GACCATCAGT 
GCTGGAGAGA GCAGAGTGAA GAACAGTCCA GCCAAACCCA GTGACAACAG CATGTCACAG 
CTGAGCCGCA GCAGCGTCGA CGCTGATCCA CTCAAGGAGC CTGAAAGTGC AGACAAACAG 
AAGAAGCAGA CAGGGAACCC ACATATTGAG CTGCAGTTCT CCGATGCAAA TGCTAAGTTC 
TACTGCCGGA TCTACTATGC TGAAGAGTTC CATAAAATGC GGGAGGAGAT CATGGAGAGC 
ACAGAGGATG ATTTTGTGCG ATCTCTTTCC CACTGTGTCA ACTGGCAGGC GCGTGGTGGG 
AAATCTGGTG CAGTTTTCTA TGCCACTGAA GATGATCGGT TTATTCTGAA GCAGATGCCC 
AGATTAGAGG TCCAGTCCTT TTTAGACTTT GCACCCCACT ACTTTACGTA CATCACAGGA 
GCGGTTCACC TAAAGCGACC CACAGCACTT GCTAAGATTC TGGGAGTGTA CCGCATCGGC 
TACAAGAACT CCCAGAACAA CACTGAGAAG AAGCTTGACC TGCTTGTGAT GGAAAACCTT 
TTCTATGGAC GAAAGATGGC ACAGGTGTTT GACCTGAAAG GATCGCTGAG AAACAGGAAC 
GTGAAGACGG ACCAGGGAAA GGAGAGCTGT GAGGTGGTGC TCCTAGATGA GAACCTGCTC 
AAACTGGTGC ATGACAATCC CCTTTATATT CGCTCGCACT GCAAGGCCAT CCTGCGTGCT 
GCCATCCACA GCGACGCGCT CTTCCTGTCC AGCCACCTCA TCATTGATTA CTCCCTGCTG 
GTGGGCCGAG ACGATTCCAC AGATGAGCTA GTTGTGGGGA TCATAGATTA TATCCGGACT 
TTCACTTGGG ATAAAAAGCT GGAGATGGTG GTCAAATCTA CTGGGATTCT TGGAGGTCAA 
GGTAAAATGC CCACAGTGGT GTCACCAGAG CTGTATCGAA CACGTTTCTG TGAGGCCATG 
GATAAATACT TCCTTATGGT CCCTGATCAC TGGAGTGGAC TTGGGCTCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304726.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303001.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X11, mRNA.

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CloneID	ODa40127
Clone ID Related Accession (Same CDS sequence)	XM_009304726.3 , XM_009304726.2
Accession Version	XM_009304726.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACATC ATTCCTTTTA TTTTTTTGTT GCATTCATGA GGGAATTTCC GTCTGACAAC 
AAATTACATC ATCAGGAATC TCAGAACAGC GGTCTCAATT CCAGACTGAC AGTGGTCCAA 
GAGGATATTG GAAAGTCACC TGCCAGGAAG AGGTCAGCTA GTGTGACCAA TTTGTCTTTG 
GACCGTTCCG GCTCATCCAT GGTTCCTGCC TATGAGAGTT CGGTTAGTCC ACAGAACAGC 
CGAGCTCTAC CCAAGACCGA CCACAGCGAA GAAGAGCGAA AGATCCTTCT AGATTCTTTT 
CAACTTAAAG ATATATGGAA GAAGATTTGC CACAACACTA CTGGGATGGA GTTCCAGGAC 
CATCGGTACT GGCTGCGCAC TTACCCCAAC TGCATTGTGG GTAAAGAGTT GGTCAACTGG 
CTTTTAAGAA ATGGCACTAT TTCCACTAGG GCTCAAGCCA TAGCTATAGG ACAGGCTTTG 
GTAGATGGCC GCTGGCTTGA CTGTGTAACC CATCATGATC AGATCTTCCG TGATGAGTAC 
GCCCTTTATC GCCCCCTTCA GAGCACAGAG TTCTCCGAAA CTCCTTCTCC AGACAGTGAC 
AGTGTTAATT CCCTGGAGGG ACACTCTGAA CCGTCATGGT TTAAAGACAT CAAGTTTGAT 
GACAGTGACA CCGAGCAGCT AGCAGATGAA AATGACTATG TCACACCAAG CTCGTCCAGC 
CCCAGCAAAA GAACATCTGT CAGCAGTTTC CACTCTGCAG TAGACAGTGA CTCGGCCGCC 
TCCATCAGTC TAAACATGGA GCAGGACAAC GTCAATTTCC ACATTAAGAA GCAGGCCAAG 
TACCCCCATG TGCCTCCCTA CCCAGCTGAG CAAAAAAGTA TGGAGCCCCA TGACCCGTTC 
ACCCCAGAAA CCGACATCCA TGCACCAATG GAAGTCTTGC TCTCCGAAGA TGGAGGTCAG 
CATATATCCA TCAGTGATGC ATTCATCAAA GAGTCTTTGT TCAACCGGAG GGTTGAGGAG 
AAAGCTAAAG AGATGCTCTT CACTCCTCTC GGCTGGCATC ACAGCTCTCT GGATCAGCTG 
CGGGAAGAGA ATGGGGAAAA GAAAGCGATG GAGCGGTTGC TGTCAGCAAA TCACAGCCAC 
ATGATGGCGC TGCTGCAGCA ACTGCTGTAC AGTGAATCAC TGTCTCTGTC GTGGCGTGAC 
ATCATCGTAC CTGTGGTGAG GCAGGTGGTG CAGACGGTGC GACCGGATGT GCGCAGCTGT 
GACGATGATA TGGACATCCG TCAGTTTGTC CACATCAAGA AGATTCCAGG AGGAAAGAAG 
TTTGACTCTG CTGTAGTGAA TGGTTTTGTT TGCACAAAGA ATATCGCACA CAAAAAGATG 
AACTCTTACA TCAAAAACCC CAAGATCCTT CTTCTCAAAT GCTCCATTGA GTATCTATAT 
AGAGAGGAGA CCAAGTTCAC CTGCATTGAC CCAATTGTTT TACAGGAGCG TGAGTTTCTT 
AAGAACTATG TTCAGAGGAT TGTTGATGTC CGACCAAACT TAGTCCTGGT GGAGAAGACT 
GTATCGCGAA TTGCTCAGGA CATGCTACTA GAGCACGGCA TTGCCATTGT GATTAATGTC 
AAACCTCAAG TACTGGACCG TGTGAGTCGA ATGACTCAGG GAGATTTAGT CATTTCAATG 
GATCAGCTTC TTACAAAACC TCGTCTGGGT ACCTGCCACA AGTTTTACTT GCACTCCTTC 
CAGCTACCAA ATAGTGAAGT GAAAACCCTG ATGTTTTTTG AGGGCTGTCC TCCTCAGCTG 
GGCTGCACTA TTAAGCTCCG CGGAGCCTCA GAGTATGAGC TGGCACGGGT AAAAGAGATC 
ATCATTTTTA TGGTGTGTGT GGCATACCAC TCGCAGCTAG AGATCTCTTT CCTCATGGAT 
GAGTTCGCTA TGCCTCCCAG TCTGGCTGAG AGTTCTTCTT TCCCATGTCT GCTGGAAAGC 
ACCACTTTAC GGGAAGAGGA TGAAACTAGT GGGGAACGGG AAAGCCAAGA CAATGATGAG 
TCTACACTGG GAGATGACAA TCTCACACTT GTTCCAGGAG ATGACTTTGA GACAGGATTT 
ACAGGGACTA TCAAAGCCCA CAGTCGAGAG CCTTCCACCT CTGAATCTTC TCATAAAGAT 
GGAGAAAGCC CCAGAATAAA TAAAAATGCT TCAATTACCT CCTTCTCTGG AGGGGAGGAA 
GAAAATATAA AGACCTCCAC GCCACTTTCC TCCTTCTCTT CCACCTTTCC TCAGCCGGTG 
ACACCACCTT TCCTGATATC AGACGTAAAA GAAATGTCAC AGGAAGTGGT CAAGGGGTCA 
GGTGAAGGGG AGAAGAACAA ATATCTGGAG TCGACTCTGG TCCATCAGGA CAGCACAAGC 
TCTGAGACCT CCCTTCCCCC AACTCGGCTC TTTAGAGACC CCTTACAGGA CGACACAGGC 
CTGTTTGTGA CAGAGCATGT AGCTTCTTCA GATGACCGCC TCAAATCCAT CTCTGCTTTA 
TTCAAACAGG AGCTAAAAGA CATCATCCTG TGCATTTCAC CCTTTATTAC CTTTCGAGAG 
CCTTTCTTGC TTACCCCAGC TGGACTGCGG TGTCCTAGCC GGGATTATTT CCCTGAACAG 
GTTTACCTTT CACCTCTGCT GAACAAGGAT TCAAAAGAAC TGGATGGACG CCGCAAGAGG 
CAGCTGCTGA AAGAGTCCAG CCCAGGTTCT GGTAGCCTGA CCAATGGTTC CGTTTCACAC 
CAGAGAGCCA TTCAGATCTT GCCCTGTCAC AAACTCACTA GTGCCCGAAT AGCAGAACAG 
GTAGGCAGCA GTCAGCAGCT GGCACGCATG TTAGCGGACT ACCGTGCCCA AGGAGGACGT 
ATCCGGCAAA GAGAGGGAAC ACAATTTCGT GAAGCCCCTC CCCCAAAGGC GCCAATAAAG 
GCAGATAGTG AAGAAGATAA AGGTGCAGGA GTGATTGACA TGACCTGGTC TACTAAGTTG 
GACTGTTTAA ATCCAATCAA CCATCAGAGG CTCTGTGTTC TGTTCAGTAG CTCTTCAGCA 
CAGTCTAATA ATGCCCCAAA CCCCTGCGTC AGTCCCTGGA TTGTTACAAT GGAGTTTTAT 
GGAAAGAATG ACTTGACTCT TGGTGTGTTT CTGGAGAGAT ACTGTTTTAG ACCATCTTAC 
CAGTGCCCCA GCATGTACTG TGAGACCCCC ATGGTTCACC ACATCCGGCG CTTTGTGCAC 
GGTAAAGGCT GCGTCCAGAT TGTACTGAAA GAGCTGGACT CGCCTGTTCC TGGATATCAG 
CACACAATCC TCAACTACTC ATGGTGTCGC ATCTGCAAAC AGGTGACTCC TGTGGTCCCT 
TTGTCTAATG ACTCTTGGTC CATGTCCTTC GCCAAGTACC TTGAGCTTCG TTTCTATGGT 
CATCAGTACA CCCGTCGGGC TAACGCTGAG CCTTGTGGCC ACTCTATCCA TAAAGATTAT 
CACCAGTACT TCTCTTACAA CCAGATGGTG GCATCCTTCA GCTACATTCC AGTGAGGCTG 
TTAGAGATCT GTCTGCCTCC CCCGAAGATC ATCATCAGGA ACCAAGGTCC ATCGAAATCC 
AGCTTGCAGC AGGATCTCAA AGACTTCTCC CAGAAGGTGG CTCAGGTGTA CCTGGCTATA 
GATGACCGTC TTACCTCCTT AAAAACGGAC ACATTCAGCA AGACCAGAGA GGAAAAAATG 
GAGGACATGT TTGCACAGAA AGATATGGAG GAATCAGAGC TTCGGGGCTG GATAGAGAAG 
TTACAAGTGC GTCTGCAGAC GAGTGTGATT GACTCTTCAC AACAACTACA GGCTGTTCTG 
GAGTCAGTGG TGGTCAAAAA ACAGGGCTTG TGTGAAACCC TGCAGTCCTG GAACAACAGA 
CTTCAAGAAC TGTTCCAGCA AGAGAAAGGC AGAAAACGTC TATCTGTTCC TCCTAGCCCA 
GGCAGACACA GACAACCTAC AACAGATGAG AGCAAGACTA GTGCTTTGGA GTCTTCTCCT 
CGTAACCCTT CCCCAGTGGT CCCAAATGGA GACAAAGAGG ACCGTCATCT CAATACATTT 
CCATCAAGTT CAGGGTCCTC GTCGTTACTA CAGTTACCAT CTCCAGCTGA ACAACCTTCA 
GATGTCATTA CAAGCGGACC ATCTTTCCCT GATCAGGACT CTGTTAGCAA TCCAGAGGAC 
ATGTTTGATG GACACTTGCT TGGTTCCAAT GATAGTCAGG TAAAGGAAAA GTCCACCATG 
AAAACAATCC TGGCCAACCT GTTACCTGGA AACAGCTACA ATCCCATCCC TTTTCCTTTT 
GACCCAGACA AGCACTATCT GATGTATGAG CATGAGAGAG TTCCTATAGC CGTGTGTGAG 
AGAGAGCCCA GCTCCATAAT TGCCTTTGCA CTCAGCTGCA AAGAGTATAA AACTGCCCTT 
GAAGAGCTCA CAAAGACAAC AGCAAAGACT GGAGGTGATG ACACTTCTCA GACCATCAGT 
GCTGGAGAGA GCAGAGTGAA GAACAGTCCA GCCAAACCCA GTGACAACAG CATGTCACAG 
CTGAGCCGCA GCAGCGTCGA CGCTGATCCA CTCAAGGAGC CTGAAAGTGC AGACAAACAG 
AAGAAGCAGA CAGGGAACCC ACATATTGAG CTGCAGTTCT CCGATGCAAA TGCTAAGTTC 
TACTGCCGGA TCTACTATGC TGAAGAGTTC CATAAAATGC GGGAGGAGAT CATGGAGAGC 
ACAGAGGATG ATTTTGTGCG ATCTCTTTCC CACTGTGTCA ACTGGCAGGC GCGTGGTGGG 
AAATCTGGTG CAGTTTTCTA TGCCACTGAA GATGATCGGT TTATTCTGAA GCAGATGCCC 
AGATTAGAGG TCCAGTCCTT TTTAGACTTT GCACCCCACT ACTTTACGTA CATCACAGGA 
GCGGTTCACC TAAAGCGACC CACAGCACTT GCTAAGATTC TGGGAGTGTA CCGCATCGGC 
TACAAGAACT CCCAGAACAA CACTGAGAAG AAGCTTGACC TGCTTGTGAT GGAAAACCTT 
TTCTATGGAC GAAAGATGGC ACAGGTGTTT GACCTGAAAG GATCGCTGAG AAACAGGAAC 
GTGAAGACGG ACCAGGGAAA GGAGAGCTGT GAGGTGGTGC TCCTAGATGA GAACCTGCTC 
AAACTGGTGC ATGACAATCC CCTTTATATT CGCTCGCACT GCAAGGCCAT CCTGCGTGCT 
GCCATCCACA GCGACGCGCT CTTCCTGTCC AGCCACCTCA TCATTGATTA CTCCCTGCTG 
GTGGGCCGAG ACGATTCCAC AGATGAGCTA GTTGTGGGGA TCATAGATTA TATCCGGACT 
TTCACTTGGG ATAAAAAGCT GGAGATGGTG GTCAAATCTA CTGGGATTCT TGGAGGTCAA 
GGTAAAATGC CCACAGTGGT GTCACCAGAG CTGTATCGAA CACGTTTCTG TGAGGCCATG 
GATAAATACT TCCTTATGGT CCCTGATCAC TGGAGTGGAC TTGGGCTCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303001.1
CDS	521..6037
Translation	MVHHSFYFFVAFMREFPSDNKLHHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304726.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304726.2

ORF Insert Sequence:

ATGGTACATC ATTCCTTTTA TTTTTTTGTT GCATTCATGA GGGAATTTCC GTCTGACAAC 
AAATTACATC ATCAGGAATC TCAGAACAGC GGTCTCAATT CCAGACTGAC AGTGGTCCAA 
GAGGATATTG GAAAGTCACC TGCCAGGAAG AGGTCAGCTA GTGTGACCAA TTTGTCTTTG 
GACCGTTCCG GCTCATCCAT GGTTCCTGCC TATGAGAGTT CGGTTAGTCC ACAGAACAGC 
CGAGCTCTAC CCAAGACCGA CCACAGCGAA GAAGAGCGAA AGATCCTTCT AGATTCTTTT 
CAACTTAAAG ATATATGGAA GAAGATTTGC CACAACACTA CTGGGATGGA GTTCCAGGAC 
CATCGGTACT GGCTGCGCAC TTACCCCAAC TGCATTGTGG GTAAAGAGTT GGTCAACTGG 
CTTTTAAGAA ATGGCACTAT TTCCACTAGG GCTCAAGCCA TAGCTATAGG ACAGGCTTTG 
GTAGATGGCC GCTGGCTTGA CTGTGTAACC CATCATGATC AGATCTTCCG TGATGAGTAC 
GCCCTTTATC GCCCCCTTCA GAGCACAGAG TTCTCCGAAA CTCCTTCTCC AGACAGTGAC 
AGTGTTAATT CCCTGGAGGG ACACTCTGAA CCGTCATGGT TTAAAGACAT CAAGTTTGAT 
GACAGTGACA CCGAGCAGCT AGCAGATGAA AATGACTATG TCACACCAAG CTCGTCCAGC 
CCCAGCAAAA GAACATCTGT CAGCAGTTTC CACTCTGCAG TAGACAGTGA CTCGGCCGCC 
TCCATCAGTC TAAACATGGA GCAGGACAAC GTCAATTTCC ACATTAAGAA GCAGGCCAAG 
TACCCCCATG TGCCTCCCTA CCCAGCTGAG CAAAAAAGTA TGGAGCCCCA TGACCCGTTC 
ACCCCAGAAA CCGACATCCA TGCACCAATG GAAGTCTTGC TCTCCGAAGA TGGAGGTCAG 
CATATATCCA TCAGTGATGC ATTCATCAAA GAGTCTTTGT TCAACCGGAG GGTTGAGGAG 
AAAGCTAAAG AGATGCTCTT CACTCCTCTC GGCTGGCATC ACAGCTCTCT GGATCAGCTG 
CGGGAAGAGA ATGGGGAAAA GAAAGCGATG GAGCGGTTGC TGTCAGCAAA TCACAGCCAC 
ATGATGGCGC TGCTGCAGCA ACTGCTGTAC AGTGAATCAC TGTCTCTGTC GTGGCGTGAC 
ATCATCGTAC CTGTGGTGAG GCAGGTGGTG CAGACGGTGC GACCGGATGT GCGCAGCTGT 
GACGATGATA TGGACATCCG TCAGTTTGTC CACATCAAGA AGATTCCAGG AGGAAAGAAG 
TTTGACTCTG CTGTAGTGAA TGGTTTTGTT TGCACAAAGA ATATCGCACA CAAAAAGATG 
AACTCTTACA TCAAAAACCC CAAGATCCTT CTTCTCAAAT GCTCCATTGA GTATCTATAT 
AGAGAGGAGA CCAAGTTCAC CTGCATTGAC CCAATTGTTT TACAGGAGCG TGAGTTTCTT 
AAGAACTATG TTCAGAGGAT TGTTGATGTC CGACCAAACT TAGTCCTGGT GGAGAAGACT 
GTATCGCGAA TTGCTCAGGA CATGCTACTA GAGCACGGCA TTGCCATTGT GATTAATGTC 
AAACCTCAAG TACTGGACCG TGTGAGTCGA ATGACTCAGG GAGATTTAGT CATTTCAATG 
GATCAGCTTC TTACAAAACC TCGTCTGGGT ACCTGCCACA AGTTTTACTT GCACTCCTTC 
CAGCTACCAA ATAGTGAAGT GAAAACCCTG ATGTTTTTTG AGGGCTGTCC TCCTCAGCTG 
GGCTGCACTA TTAAGCTCCG CGGAGCCTCA GAGTATGAGC TGGCACGGGT AAAAGAGATC 
ATCATTTTTA TGGTGTGTGT GGCATACCAC TCGCAGCTAG AGATCTCTTT CCTCATGGAT 
GAGTTCGCTA TGCCTCCCAG TCTGGCTGAG AGTTCTTCTT TCCCATGTCT GCTGGAAAGC 
ACCACTTTAC GGGAAGAGGA TGAAACTAGT GGGGAACGGG AAAGCCAAGA CAATGATGAG 
TCTACACTGG GAGATGACAA TCTCACACTT GTTCCAGGAG ATGACTTTGA GACAGGATTT 
ACAGGGACTA TCAAAGCCCA CAGTCGAGAG CCTTCCACCT CTGAATCTTC TCATAAAGAT 
GGAGAAAGCC CCAGAATAAA TAAAAATGCT TCAATTACCT CCTTCTCTGG AGGGGAGGAA 
GAAAATATAA AGACCTCCAC GCCACTTTCC TCCTTCTCTT CCACCTTTCC TCAGCCGGTG 
ACACCACCTT TCCTGATATC AGACGTAAAA GAAATGTCAC AGGAAGTGGT CAAGGGGTCA 
GGTGAAGGGG AGAAGAACAA ATATCTGGAG TCGACTCTGG TCCATCAGGA CAGCACAAGC 
TCTGAGACCT CCCTTCCCCC AACTCGGCTC TTTAGAGACC CCTTACAGGA CGACACAGGC 
CTGTTTGTGA CAGAGCATGT AGCTTCTTCA GATGACCGCC TCAAATCCAT CTCTGCTTTA 
TTCAAACAGG AGCTAAAAGA CATCATCCTG TGCATTTCAC CCTTTATTAC CTTTCGAGAG 
CCTTTCTTGC TTACCCCAGC TGGACTGCGG TGTCCTAGCC GGGATTATTT CCCTGAACAG 
GTTTACCTTT CACCTCTGCT GAACAAGGAT TCAAAAGAAC TGGATGGACG CCGCAAGAGG 
CAGCTGCTGA AAGAGTCCAG CCCAGGTTCT GGTAGCCTGA CCAATGGTTC CGTTTCACAC 
CAGAGAGCCA TTCAGATCTT GCCCTGTCAC AAACTCACTA GTGCCCGAAT AGCAGAACAG 
GTAGGCAGCA GTCAGCAGCT GGCACGCATG TTAGCGGACT ACCGTGCCCA AGGAGGACGT 
ATCCGGCAAA GAGAGGGAAC ACAATTTCGT GAAGCCCCTC CCCCAAAGGC GCCAATAAAG 
GCAGATAGTG AAGAAGATAA AGGTGCAGGA GTGATTGACA TGACCTGGTC TACTAAGTTG 
GACTGTTTAA ATCCAATCAA CCATCAGAGG CTCTGTGTTC TGTTCAGTAG CTCTTCAGCA 
CAGTCTAATA ATGCCCCAAA CCCCTGCGTC AGTCCCTGGA TTGTTACAAT GGAGTTTTAT 
GGAAAGAATG ACTTGACTCT TGGTGTGTTT CTGGAGAGAT ACTGTTTTAG ACCATCTTAC 
CAGTGCCCCA GCATGTACTG TGAGACCCCC ATGGTTCACC ACATCCGGCG CTTTGTGCAC 
GGTAAAGGCT GCGTCCAGAT TGTACTGAAA GAGCTGGACT CGCCTGTTCC TGGATATCAG 
CACACAATCC TCAACTACTC ATGGTGTCGC ATCTGCAAAC AGGTGACTCC TGTGGTCCCT 
TTGTCTAATG ACTCTTGGTC CATGTCCTTC GCCAAGTACC TTGAGCTTCG TTTCTATGGT 
CATCAGTACA CCCGTCGGGC TAACGCTGAG CCTTGTGGCC ACTCTATCCA TAAAGATTAT 
CACCAGTACT TCTCTTACAA CCAGATGGTG GCATCCTTCA GCTACATTCC AGTGAGGCTG 
TTAGAGATCT GTCTGCCTCC CCCGAAGATC ATCATCAGGA ACCAAGGTCC ATCGAAATCC 
AGCTTGCAGC AGGATCTCAA AGACTTCTCC CAGAAGGTGG CTCAGGTGTA CCTGGCTATA 
GATGACCGTC TTACCTCCTT AAAAACGGAC ACATTCAGCA AGACCAGAGA GGAAAAAATG 
GAGGACATGT TTGCACAGAA AGATATGGAG GAATCAGAGC TTCGGGGCTG GATAGAGAAG 
TTACAAGTGC GTCTGCAGAC GAGTGTGATT GACTCTTCAC AACAACTACA GGCTGTTCTG 
GAGTCAGTGG TGGTCAAAAA ACAGGGCTTG TGTGAAACCC TGCAGTCCTG GAACAACAGA 
CTTCAAGAAC TGTTCCAGCA AGAGAAAGGC AGAAAACGTC TATCTGTTCC TCCTAGCCCA 
GGCAGACACA GACAACCTAC AACAGATGAG AGCAAGACTA GTGCTTTGGA GTCTTCTCCT 
CGTAACCCTT CCCCAGTGGT CCCAAATGGA GACAAAGAGG ACCGTCATCT CAATACATTT 
CCATCAAGTT CAGGGTCCTC GTCGTTACTA CAGTTACCAT CTCCAGCTGA ACAACCTTCA 
GATGTCATTA CAAGCGGACC ATCTTTCCCT GATCAGGACT CTGTTAGCAA TCCAGAGGAC 
ATGTTTGATG GACACTTGCT TGGTTCCAAT GATAGTCAGG TAAAGGAAAA GTCCACCATG 
AAAACAATCC TGGCCAACCT GTTACCTGGA AACAGCTACA ATCCCATCCC TTTTCCTTTT 
GACCCAGACA AGCACTATCT GATGTATGAG CATGAGAGAG TTCCTATAGC CGTGTGTGAG 
AGAGAGCCCA GCTCCATAAT TGCCTTTGCA CTCAGCTGCA AAGAGTATAA AACTGCCCTT 
GAAGAGCTCA CAAAGACAAC AGCAAAGACT GGAGGTGATG ACACTTCTCA GACCATCAGT 
GCTGGAGAGA GCAGAGTGAA GAACAGTCCA GCCAAACCCA GTGACAACAG CATGTCACAG 
CTGAGCCGCA GCAGCGTCGA CGCTGATCCA CTCAAGGAGC CTGAAAGTGC AGACAAACAG 
AAGAAGCAGA CAGGGAACCC ACATATTGAG CTGCAGTTCT CCGATGCAAA TGCTAAGTTC 
TACTGCCGGA TCTACTATGC TGAAGAGTTC CATAAAATGC GGGAGGAGAT CATGGAGAGC 
ACAGAGGATG ATTTTGTGCG ATCTCTTTCC CACTGTGTCA ACTGGCAGGC GCGTGGTGGG 
AAATCTGGTG CAGTTTTCTA TGCCACTGAA GATGATCGGT TTATTCTGAA GCAGATGCCC 
AGATTAGAGG TCCAGTCCTT TTTAGACTTT GCACCCCACT ACTTTACGTA CATCACAGGA 
GCGGTTCACC TAAAGCGACC CACAGCACTT GCTAAGATTC TGGGAGTGTA CCGCATCGGC 
TACAAGAACT CCCAGAACAA CACTGAGAAG AAGCTTGACC TGCTTGTGAT GGAAAACCTT 
TTCTATGGAC GAAAGATGGC ACAGGTGTTT GACCTGAAAG GATCGCTGAG AAACAGGAAC 
GTGAAGACGG ACCAGGGAAA GGAGAGCTGT GAGGTGGTGC TCCTAGATGA GAACCTGCTC 
AAACTGGTGC ATGACAATCC CCTTTATATT CGCTCGCACT GCAAGGCCAT CCTGCGTGCT 
GCCATCCACA GCGACGCGCT CTTCCTGTCC AGCCACCTCA TCATTGATTA CTCCCTGCTG 
GTGGGCCGAG ACGATTCCAC AGATGAGCTA GTTGTGGGGA TCATAGATTA TATCCGGACT 
TTCACTTGGG ATAAAAAGCT GGAGATGGTG GTCAAATCTA CTGGGATTCT TGGAGGTCAA 
GGTAAAATGC CCACAGTGGT GTCACCAGAG CTGTATCGAA CACGTTTCTG TGAGGCCATG 
GATAAATACT TCCTTATGGT CCCTGATCAC TGGAGTGGAC TTGGGCTCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304721.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302996.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.

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CloneID	ODa40122
Clone ID Related Accession (Same CDS sequence)	XM_009304721.3 , XM_009304721.2
Accession Version	XM_009304721.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAAGTAT GGAGCCCCAT 
GACCCGTTCA CCCCAGAAAC CGACATCCAT GCACCAATGG AAGTCTTGCT CTCCGAAGAT 
GGAGGTCAGC ATATATCCAT CAGTGATGCA TTCATCAAAG AGTCTTTGTT CAACCGGAGG 
GTTGAGGAGA AAGCTAAAGA GATGCTCTTC ACTCCTCTCG GCTGGCATCA CAGCTCTCTG 
GATCAGCTGC GGGAAGAGAA TGGGGAAAAG AAAGCGATGG AGCGGTTGCT GTCAGCAAAT 
CACAGCCACA TGATGGCGCT GCTGCAGCAA CTGCTGTACA GTGAATCACT GTCTCTGTCG 
TGGCGTGACA TCATCGTACC TGTGGTGAGG CAGGTGGTGC AGACGGTGCG ACCGGATGTG 
CGCAGCTGTG ACGATGATAT GGACATCCGT CAGTTTGTCC ACATCAAGAA GATTCCAGGA 
GGAAAGAAGT TTGACTCTGC TGTAGTGAAT GGTTTTGTTT GCACAAAGAA TATCGCACAC 
AAAAAGATGA ACTCTTACAT CAAAAACCCC AAGATCCTTC TTCTCAAATG CTCCATTGAG 
TATCTATATA GAGAGGAGAC CAAGTTCACC TGCATTGACC CAATTGTTTT ACAGGAGCGT 
GAGTTTCTTA AGAACTATGT TCAGAGGATT GTTGATGTCC GACCAAACTT AGTCCTGGTG 
GAGAAGACTG TATCGCGAAT TGCTCAGGAC ATGCTACTAG AGCACGGCAT TGCCATTGTG 
ATTAATGTCA AACCTCAAGT ACTGGACCGT GTGAGTCGAA TGACTCAGGG AGATTTAGTC 
ATTTCAATGG ATCAGCTTCT TACAAAACCT CGTCTGGGTA CCTGCCACAA GTTTTACTTG 
CACTCCTTCC AGCTACCAAA TAGTGAAGTG AAAACCCTGA TGTTTTTTGA GGGCTGTCCT 
CCTCAGCTGG GCTGCACTAT TAAGCTCCGC GGAGCCTCAG AGTATGAGCT GGCACGGGTA 
AAAGAGATCA TCATTTTTAT GGTGTGTGTG GCATACCACT CGCAGCTAGA GATCTCTTTC 
CTCATGGATG AGTTCGCTAT GCCTCCCAGT CTGGCTGAGA GTTCTTCTTT CCCATGTCTG 
CTGGAAAGCA CCACTTTACG GGAAGAGGAT GAAACTAGTG GGGAACGGGA AAGCCAAGAC 
AATGATGAGT CTACACTGGG AGATGACAAT CTCACACTTG TTCCAGGAGA TGACTTTGAG 
ACAGGATTTA CAGGGACTAT CAAAGCCCAC AGTCGAGAGC CTTCCACCTC TGAATCTTCT 
CATAAAGATG GAGAAAGCCC CAGAATAAAT AAAAATGCTT CAATTACCTC CTTCTCTGGA 
GGGGAGGAAG AAAATATAAA GACCTCCACG CCACTTTCCT CCTTCTCTTC CACCTTTCCT 
CAGCCGGTGA CACCACCTTT CCTGATATCA GACGTAAAAG AAATGTCACA GGAAGTGGTC 
AAGGGGTCAG GTGAAGGGGA GAAGAACAAA TATCTGGAGT CGACTCTGGT CCATCAGGAC 
AGCACAAGCT CTGAGACCTC CCTTCCCCCA ACTCGGCTCT TTAGAGACCC CTTACAGGAC 
GACACAGGCC TGTTTGTGAC AGAGCATGTA GCTTCTTCAG ATGACCGCCT CAAATCCATC 
TCTGCTTTAT TCAAACAGGA GCTAAAAGAC ATCATCCTGT GCATTTCACC CTTTATTACC 
TTTCGAGAGC CTTTCTTGCT TACCCCAGCT GGACTGCGGT GTCCTAGCCG GGATTATTTC 
CCTGAACAGG TTTACCTTTC ACCTCTGCTG AACAAGGATT CAAAAGAACT GGATGGACGC 
CGCAAGAGGC AGCTGCTGAA AGAGTCCAGC CCAGGTTCTG GTAGCCTGAC CAATGGTTCC 
GTTTCACACC AGAGAGCCAT TCAGATCTTG CCCTGTCACA AACTCACTAG TGCCCGAATA 
GCAGAACAGG TAGGCAGCAG TCAGCAGCTG GCACGCATGT TAGCGGACTA CCGTGCCCAA 
GGAGGACGTA TCCGGCAAAG AGAGGGAACA CAATTTCGTG AAGCCCCTCC CCCAAAGGCG 
CCAATAAAGG CAGATAGTGA AGAAGATAAA GGTGCAGGAG TGATTGACAT GACCTGGTCT 
ACTAAGTTGG ACTGTTTAAA TCCAATCAAC CATCAGAGGC TCTGTGTTCT GTTCAGTAGC 
TCTTCAGCAC AGTCTAATAA TGCCCCAAAC CCCTGCGTCA GTCCCTGGAT TGTTACAATG 
GAGTTTTATG GAAAGAATGA CTTGACTCTT GGTGTGTTTC TGGAGAGATA CTGTTTTAGA 
CCATCTTACC AGTGCCCCAG CATGTACTGT GAGACCCCCA TGGTTCACCA CATCCGGCGC 
TTTGTGCACG GTAAAGGCTG CGTCCAGATT GTACTGAAAG AGCTGGACTC GCCTGTTCCT 
GGATATCAGC ACACAATCCT CAACTACTCA TGGTGTCGCA TCTGCAAACA GGTGACTCCT 
GTGGTCCCTT TGTCTAATGA CTCTTGGTCC ATGTCCTTCG CCAAGTACCT TGAGCTTCGT 
TTCTATGGTC ATCAGTACAC CCGTCGGGCT AACGCTGAGC CTTGTGGCCA CTCTATCCAT 
AAAGATTATC ACCAGTACTT CTCTTACAAC CAGATGGTGG CATCCTTCAG CTACATTCCA 
GTGAGGCTGT TAGAGATCTG TCTGCCTCCC CCGAAGATCA TCATCAGGAA CCAAGGTCCA 
TCGAAATCCA GCTTGCAGCA GGATCTCAAA GACTTCTCCC AGAAGGTGGC TCAGGTGTAC 
CTGGCTATAG ATGACCGTCT TACCTCCTTA AAAACGGACA CATTCAGCAA GACCAGAGAG 
GAAAAAATGG AGGACATGTT TGCACAGAAA GATATGGAGG AATCAGAGCT TCGGGGCTGG 
ATAGAGAAGT TACAAGTGCG TCTGCAGACG AGTGTGATTG ACTCTTCACA ACAACTACAG 
GCTGTTCTGG AGTCAGTGGT GGTCAAAAAA CAGGGCTTGT GTGAAACCCT GCAGTCCTGG 
AACAACAGAC TTCAAGAACT GTTCCAGCAA GAGAAAGGCA GAAAACGTCT ATCTGTTCCT 
CCTAGCCCAG GCAGACACAG ACAACCTACA ACAGATGAGA GCAAGACTAG TGCTTTGGAG 
TCTTCTCCTC GTAACCCTTC CCCAGTGGTC CCAAATGGAG ACAAAGAGGA CCGTCATCTC 
AATACATTTC CATCAAGTTC AGGGTCCTCG TCGTTACTAC AGTTACCATC TCCAGCTGAA 
CAACCTTCAG ATGTCATTAC AAGCGGACCA TCTTTCCCTG ATCAGGACTC TGTTAGCAAT 
CCAGAGGACA TGTTTGATGG ACACTTGCTT GGTTCCAATG ATAGTCAGGT AAAGGAAAAG 
TCCACCATGA AAACAATCCT GGCCAACCTG TTACCTGGAA ACAGCTACAA TCCCATCCCT 
TTTCCTTTTG ACCCAGACAA GCACTATCTG ATGTATGAGC ATGAGAGAGT TCCTATAGCC 
GTGTGTGAGA GAGAGCCCAG CTCCATAATT GCCTTTGCAC TCAGCTGCAA AGAGTATAAA 
ACTGCCCTTG AAGAGCTCAC AAAGACAACA GCAAAGACTG GAGGTGATGA CACTTCTCAG 
ACCATCAGTG CTGGAGAGAG CAGAGTGAAG AACAGTCCAG CCAAACCCAG TGACAACAGC 
ATGTCACAGC TGAGCCGCAG CAGCGTCGAC GCTGATCCAC TCAAGGAGCC TGAAAGTGCA 
GACAAACAGA AGAAGCAGAC AGGGAACCCA CATATTGAGC TGCAGTTCTC CGATGCAAAT 
GCTAAGTTCT ACTGCCGGAT CTACTATGCT GAAGAGTTCC ATAAAATGCG GGAGGAGATC 
ATGGAGAGCA CAGAGGATGA TTTTGTGCGA TCTCTTTCCC ACTGTGTCAA CTGGCAGGCG 
CGTGGTGGGA AATCTGGTGC AGTTTTCTAT GCCACTGAAG ATGATCGGTT TATTCTGAAG 
CAGATGCCCA GATTAGAGGT CCAGTCCTTT TTAGACTTTG CACCCCACTA CTTTACGTAC 
ATCACAGGAG CGGTTCACCT AAAGCGACCC ACAGCACTTG CTAAGATTCT GGGAGTGTAC 
CGCATCGGCT ACAAGAACTC CCAGAACAAC ACTGAGAAGA AGCTTGACCT GCTTGTGATG 
GAAAACCTTT TCTATGGACG AAAGATGGCA CAGGTGTTTG ACCTGAAAGG ATCGCTGAGA 
AACAGGAACG TGAAGACGGA CCAGGGAAAG GAGAGCTGTG AGGTGGTGCT CCTAGATGAG 
AACCTGCTCA AACTGGTGCA TGACAATCCC CTTTATATTC GCTCGCACTG CAAGGCCATC 
CTGCGTGCTG CCATCCACAG CGACGCGCTC TTCCTGTCCA GCCACCTCAT CATTGATTAC 
TCCCTGCTGG TGGGCCGAGA CGATTCCACA GATGAGCTAG TTGTGGGGAT CATAGATTAT 
ATCCGGACTT TCACTTGGGA TAAAAAGCTG GAGATGGTGG TCAAATCTAC TGGGATTCTT 
GGAGGTCAAG GTAAAATGCC CACAGTGGTG TCACCAGAGC TGTATCGAAC ACGTTTCTGT 
GAGGCCATGG ATAAATACTT CCTTATGGTC CCTGATCACT GGAGTGGACT TGGGCTCAAC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302996.1
CDS	164..6529
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304721.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304721.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAAGTAT GGAGCCCCAT 
GACCCGTTCA CCCCAGAAAC CGACATCCAT GCACCAATGG AAGTCTTGCT CTCCGAAGAT 
GGAGGTCAGC ATATATCCAT CAGTGATGCA TTCATCAAAG AGTCTTTGTT CAACCGGAGG 
GTTGAGGAGA AAGCTAAAGA GATGCTCTTC ACTCCTCTCG GCTGGCATCA CAGCTCTCTG 
GATCAGCTGC GGGAAGAGAA TGGGGAAAAG AAAGCGATGG AGCGGTTGCT GTCAGCAAAT 
CACAGCCACA TGATGGCGCT GCTGCAGCAA CTGCTGTACA GTGAATCACT GTCTCTGTCG 
TGGCGTGACA TCATCGTACC TGTGGTGAGG CAGGTGGTGC AGACGGTGCG ACCGGATGTG 
CGCAGCTGTG ACGATGATAT GGACATCCGT CAGTTTGTCC ACATCAAGAA GATTCCAGGA 
GGAAAGAAGT TTGACTCTGC TGTAGTGAAT GGTTTTGTTT GCACAAAGAA TATCGCACAC 
AAAAAGATGA ACTCTTACAT CAAAAACCCC AAGATCCTTC TTCTCAAATG CTCCATTGAG 
TATCTATATA GAGAGGAGAC CAAGTTCACC TGCATTGACC CAATTGTTTT ACAGGAGCGT 
GAGTTTCTTA AGAACTATGT TCAGAGGATT GTTGATGTCC GACCAAACTT AGTCCTGGTG 
GAGAAGACTG TATCGCGAAT TGCTCAGGAC ATGCTACTAG AGCACGGCAT TGCCATTGTG 
ATTAATGTCA AACCTCAAGT ACTGGACCGT GTGAGTCGAA TGACTCAGGG AGATTTAGTC 
ATTTCAATGG ATCAGCTTCT TACAAAACCT CGTCTGGGTA CCTGCCACAA GTTTTACTTG 
CACTCCTTCC AGCTACCAAA TAGTGAAGTG AAAACCCTGA TGTTTTTTGA GGGCTGTCCT 
CCTCAGCTGG GCTGCACTAT TAAGCTCCGC GGAGCCTCAG AGTATGAGCT GGCACGGGTA 
AAAGAGATCA TCATTTTTAT GGTGTGTGTG GCATACCACT CGCAGCTAGA GATCTCTTTC 
CTCATGGATG AGTTCGCTAT GCCTCCCAGT CTGGCTGAGA GTTCTTCTTT CCCATGTCTG 
CTGGAAAGCA CCACTTTACG GGAAGAGGAT GAAACTAGTG GGGAACGGGA AAGCCAAGAC 
AATGATGAGT CTACACTGGG AGATGACAAT CTCACACTTG TTCCAGGAGA TGACTTTGAG 
ACAGGATTTA CAGGGACTAT CAAAGCCCAC AGTCGAGAGC CTTCCACCTC TGAATCTTCT 
CATAAAGATG GAGAAAGCCC CAGAATAAAT AAAAATGCTT CAATTACCTC CTTCTCTGGA 
GGGGAGGAAG AAAATATAAA GACCTCCACG CCACTTTCCT CCTTCTCTTC CACCTTTCCT 
CAGCCGGTGA CACCACCTTT CCTGATATCA GACGTAAAAG AAATGTCACA GGAAGTGGTC 
AAGGGGTCAG GTGAAGGGGA GAAGAACAAA TATCTGGAGT CGACTCTGGT CCATCAGGAC 
AGCACAAGCT CTGAGACCTC CCTTCCCCCA ACTCGGCTCT TTAGAGACCC CTTACAGGAC 
GACACAGGCC TGTTTGTGAC AGAGCATGTA GCTTCTTCAG ATGACCGCCT CAAATCCATC 
TCTGCTTTAT TCAAACAGGA GCTAAAAGAC ATCATCCTGT GCATTTCACC CTTTATTACC 
TTTCGAGAGC CTTTCTTGCT TACCCCAGCT GGACTGCGGT GTCCTAGCCG GGATTATTTC 
CCTGAACAGG TTTACCTTTC ACCTCTGCTG AACAAGGATT CAAAAGAACT GGATGGACGC 
CGCAAGAGGC AGCTGCTGAA AGAGTCCAGC CCAGGTTCTG GTAGCCTGAC CAATGGTTCC 
GTTTCACACC AGAGAGCCAT TCAGATCTTG CCCTGTCACA AACTCACTAG TGCCCGAATA 
GCAGAACAGG TAGGCAGCAG TCAGCAGCTG GCACGCATGT TAGCGGACTA CCGTGCCCAA 
GGAGGACGTA TCCGGCAAAG AGAGGGAACA CAATTTCGTG AAGCCCCTCC CCCAAAGGCG 
CCAATAAAGG CAGATAGTGA AGAAGATAAA GGTGCAGGAG TGATTGACAT GACCTGGTCT 
ACTAAGTTGG ACTGTTTAAA TCCAATCAAC CATCAGAGGC TCTGTGTTCT GTTCAGTAGC 
TCTTCAGCAC AGTCTAATAA TGCCCCAAAC CCCTGCGTCA GTCCCTGGAT TGTTACAATG 
GAGTTTTATG GAAAGAATGA CTTGACTCTT GGTGTGTTTC TGGAGAGATA CTGTTTTAGA 
CCATCTTACC AGTGCCCCAG CATGTACTGT GAGACCCCCA TGGTTCACCA CATCCGGCGC 
TTTGTGCACG GTAAAGGCTG CGTCCAGATT GTACTGAAAG AGCTGGACTC GCCTGTTCCT 
GGATATCAGC ACACAATCCT CAACTACTCA TGGTGTCGCA TCTGCAAACA GGTGACTCCT 
GTGGTCCCTT TGTCTAATGA CTCTTGGTCC ATGTCCTTCG CCAAGTACCT TGAGCTTCGT 
TTCTATGGTC ATCAGTACAC CCGTCGGGCT AACGCTGAGC CTTGTGGCCA CTCTATCCAT 
AAAGATTATC ACCAGTACTT CTCTTACAAC CAGATGGTGG CATCCTTCAG CTACATTCCA 
GTGAGGCTGT TAGAGATCTG TCTGCCTCCC CCGAAGATCA TCATCAGGAA CCAAGGTCCA 
TCGAAATCCA GCTTGCAGCA GGATCTCAAA GACTTCTCCC AGAAGGTGGC TCAGGTGTAC 
CTGGCTATAG ATGACCGTCT TACCTCCTTA AAAACGGACA CATTCAGCAA GACCAGAGAG 
GAAAAAATGG AGGACATGTT TGCACAGAAA GATATGGAGG AATCAGAGCT TCGGGGCTGG 
ATAGAGAAGT TACAAGTGCG TCTGCAGACG AGTGTGATTG ACTCTTCACA ACAACTACAG 
GCTGTTCTGG AGTCAGTGGT GGTCAAAAAA CAGGGCTTGT GTGAAACCCT GCAGTCCTGG 
AACAACAGAC TTCAAGAACT GTTCCAGCAA GAGAAAGGCA GAAAACGTCT ATCTGTTCCT 
CCTAGCCCAG GCAGACACAG ACAACCTACA ACAGATGAGA GCAAGACTAG TGCTTTGGAG 
TCTTCTCCTC GTAACCCTTC CCCAGTGGTC CCAAATGGAG ACAAAGAGGA CCGTCATCTC 
AATACATTTC CATCAAGTTC AGGGTCCTCG TCGTTACTAC AGTTACCATC TCCAGCTGAA 
CAACCTTCAG ATGTCATTAC AAGCGGACCA TCTTTCCCTG ATCAGGACTC TGTTAGCAAT 
CCAGAGGACA TGTTTGATGG ACACTTGCTT GGTTCCAATG ATAGTCAGGT AAAGGAAAAG 
TCCACCATGA AAACAATCCT GGCCAACCTG TTACCTGGAA ACAGCTACAA TCCCATCCCT 
TTTCCTTTTG ACCCAGACAA GCACTATCTG ATGTATGAGC ATGAGAGAGT TCCTATAGCC 
GTGTGTGAGA GAGAGCCCAG CTCCATAATT GCCTTTGCAC TCAGCTGCAA AGAGTATAAA 
ACTGCCCTTG AAGAGCTCAC AAAGACAACA GCAAAGACTG GAGGTGATGA CACTTCTCAG 
ACCATCAGTG CTGGAGAGAG CAGAGTGAAG AACAGTCCAG CCAAACCCAG TGACAACAGC 
ATGTCACAGC TGAGCCGCAG CAGCGTCGAC GCTGATCCAC TCAAGGAGCC TGAAAGTGCA 
GACAAACAGA AGAAGCAGAC AGGGAACCCA CATATTGAGC TGCAGTTCTC CGATGCAAAT 
GCTAAGTTCT ACTGCCGGAT CTACTATGCT GAAGAGTTCC ATAAAATGCG GGAGGAGATC 
ATGGAGAGCA CAGAGGATGA TTTTGTGCGA TCTCTTTCCC ACTGTGTCAA CTGGCAGGCG 
CGTGGTGGGA AATCTGGTGC AGTTTTCTAT GCCACTGAAG ATGATCGGTT TATTCTGAAG 
CAGATGCCCA GATTAGAGGT CCAGTCCTTT TTAGACTTTG CACCCCACTA CTTTACGTAC 
ATCACAGGAG CGGTTCACCT AAAGCGACCC ACAGCACTTG CTAAGATTCT GGGAGTGTAC 
CGCATCGGCT ACAAGAACTC CCAGAACAAC ACTGAGAAGA AGCTTGACCT GCTTGTGATG 
GAAAACCTTT TCTATGGACG AAAGATGGCA CAGGTGTTTG ACCTGAAAGG ATCGCTGAGA 
AACAGGAACG TGAAGACGGA CCAGGGAAAG GAGAGCTGTG AGGTGGTGCT CCTAGATGAG 
AACCTGCTCA AACTGGTGCA TGACAATCCC CTTTATATTC GCTCGCACTG CAAGGCCATC 
CTGCGTGCTG CCATCCACAG CGACGCGCTC TTCCTGTCCA GCCACCTCAT CATTGATTAC 
TCCCTGCTGG TGGGCCGAGA CGATTCCACA GATGAGCTAG TTGTGGGGAT CATAGATTAT 
ATCCGGACTT TCACTTGGGA TAAAAAGCTG GAGATGGTGG TCAAATCTAC TGGGATTCTT 
GGAGGTCAAG GTAAAATGCC CACAGTGGTG TCACCAGAGC TGTATCGAAC ACGTTTCTGT 
GAGGCCATGG ATAAATACTT CCTTATGGTC CCTGATCACT GGAGTGGACT TGGGCTCAAC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304721.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302996.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.

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CloneID	ODa40122
Clone ID Related Accession (Same CDS sequence)	XM_009304721.3 , XM_009304721.2
Accession Version	XM_009304721.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAAGTAT GGAGCCCCAT 
GACCCGTTCA CCCCAGAAAC CGACATCCAT GCACCAATGG AAGTCTTGCT CTCCGAAGAT 
GGAGGTCAGC ATATATCCAT CAGTGATGCA TTCATCAAAG AGTCTTTGTT CAACCGGAGG 
GTTGAGGAGA AAGCTAAAGA GATGCTCTTC ACTCCTCTCG GCTGGCATCA CAGCTCTCTG 
GATCAGCTGC GGGAAGAGAA TGGGGAAAAG AAAGCGATGG AGCGGTTGCT GTCAGCAAAT 
CACAGCCACA TGATGGCGCT GCTGCAGCAA CTGCTGTACA GTGAATCACT GTCTCTGTCG 
TGGCGTGACA TCATCGTACC TGTGGTGAGG CAGGTGGTGC AGACGGTGCG ACCGGATGTG 
CGCAGCTGTG ACGATGATAT GGACATCCGT CAGTTTGTCC ACATCAAGAA GATTCCAGGA 
GGAAAGAAGT TTGACTCTGC TGTAGTGAAT GGTTTTGTTT GCACAAAGAA TATCGCACAC 
AAAAAGATGA ACTCTTACAT CAAAAACCCC AAGATCCTTC TTCTCAAATG CTCCATTGAG 
TATCTATATA GAGAGGAGAC CAAGTTCACC TGCATTGACC CAATTGTTTT ACAGGAGCGT 
GAGTTTCTTA AGAACTATGT TCAGAGGATT GTTGATGTCC GACCAAACTT AGTCCTGGTG 
GAGAAGACTG TATCGCGAAT TGCTCAGGAC ATGCTACTAG AGCACGGCAT TGCCATTGTG 
ATTAATGTCA AACCTCAAGT ACTGGACCGT GTGAGTCGAA TGACTCAGGG AGATTTAGTC 
ATTTCAATGG ATCAGCTTCT TACAAAACCT CGTCTGGGTA CCTGCCACAA GTTTTACTTG 
CACTCCTTCC AGCTACCAAA TAGTGAAGTG AAAACCCTGA TGTTTTTTGA GGGCTGTCCT 
CCTCAGCTGG GCTGCACTAT TAAGCTCCGC GGAGCCTCAG AGTATGAGCT GGCACGGGTA 
AAAGAGATCA TCATTTTTAT GGTGTGTGTG GCATACCACT CGCAGCTAGA GATCTCTTTC 
CTCATGGATG AGTTCGCTAT GCCTCCCAGT CTGGCTGAGA GTTCTTCTTT CCCATGTCTG 
CTGGAAAGCA CCACTTTACG GGAAGAGGAT GAAACTAGTG GGGAACGGGA AAGCCAAGAC 
AATGATGAGT CTACACTGGG AGATGACAAT CTCACACTTG TTCCAGGAGA TGACTTTGAG 
ACAGGATTTA CAGGGACTAT CAAAGCCCAC AGTCGAGAGC CTTCCACCTC TGAATCTTCT 
CATAAAGATG GAGAAAGCCC CAGAATAAAT AAAAATGCTT CAATTACCTC CTTCTCTGGA 
GGGGAGGAAG AAAATATAAA GACCTCCACG CCACTTTCCT CCTTCTCTTC CACCTTTCCT 
CAGCCGGTGA CACCACCTTT CCTGATATCA GACGTAAAAG AAATGTCACA GGAAGTGGTC 
AAGGGGTCAG GTGAAGGGGA GAAGAACAAA TATCTGGAGT CGACTCTGGT CCATCAGGAC 
AGCACAAGCT CTGAGACCTC CCTTCCCCCA ACTCGGCTCT TTAGAGACCC CTTACAGGAC 
GACACAGGCC TGTTTGTGAC AGAGCATGTA GCTTCTTCAG ATGACCGCCT CAAATCCATC 
TCTGCTTTAT TCAAACAGGA GCTAAAAGAC ATCATCCTGT GCATTTCACC CTTTATTACC 
TTTCGAGAGC CTTTCTTGCT TACCCCAGCT GGACTGCGGT GTCCTAGCCG GGATTATTTC 
CCTGAACAGG TTTACCTTTC ACCTCTGCTG AACAAGGATT CAAAAGAACT GGATGGACGC 
CGCAAGAGGC AGCTGCTGAA AGAGTCCAGC CCAGGTTCTG GTAGCCTGAC CAATGGTTCC 
GTTTCACACC AGAGAGCCAT TCAGATCTTG CCCTGTCACA AACTCACTAG TGCCCGAATA 
GCAGAACAGG TAGGCAGCAG TCAGCAGCTG GCACGCATGT TAGCGGACTA CCGTGCCCAA 
GGAGGACGTA TCCGGCAAAG AGAGGGAACA CAATTTCGTG AAGCCCCTCC CCCAAAGGCG 
CCAATAAAGG CAGATAGTGA AGAAGATAAA GGTGCAGGAG TGATTGACAT GACCTGGTCT 
ACTAAGTTGG ACTGTTTAAA TCCAATCAAC CATCAGAGGC TCTGTGTTCT GTTCAGTAGC 
TCTTCAGCAC AGTCTAATAA TGCCCCAAAC CCCTGCGTCA GTCCCTGGAT TGTTACAATG 
GAGTTTTATG GAAAGAATGA CTTGACTCTT GGTGTGTTTC TGGAGAGATA CTGTTTTAGA 
CCATCTTACC AGTGCCCCAG CATGTACTGT GAGACCCCCA TGGTTCACCA CATCCGGCGC 
TTTGTGCACG GTAAAGGCTG CGTCCAGATT GTACTGAAAG AGCTGGACTC GCCTGTTCCT 
GGATATCAGC ACACAATCCT CAACTACTCA TGGTGTCGCA TCTGCAAACA GGTGACTCCT 
GTGGTCCCTT TGTCTAATGA CTCTTGGTCC ATGTCCTTCG CCAAGTACCT TGAGCTTCGT 
TTCTATGGTC ATCAGTACAC CCGTCGGGCT AACGCTGAGC CTTGTGGCCA CTCTATCCAT 
AAAGATTATC ACCAGTACTT CTCTTACAAC CAGATGGTGG CATCCTTCAG CTACATTCCA 
GTGAGGCTGT TAGAGATCTG TCTGCCTCCC CCGAAGATCA TCATCAGGAA CCAAGGTCCA 
TCGAAATCCA GCTTGCAGCA GGATCTCAAA GACTTCTCCC AGAAGGTGGC TCAGGTGTAC 
CTGGCTATAG ATGACCGTCT TACCTCCTTA AAAACGGACA CATTCAGCAA GACCAGAGAG 
GAAAAAATGG AGGACATGTT TGCACAGAAA GATATGGAGG AATCAGAGCT TCGGGGCTGG 
ATAGAGAAGT TACAAGTGCG TCTGCAGACG AGTGTGATTG ACTCTTCACA ACAACTACAG 
GCTGTTCTGG AGTCAGTGGT GGTCAAAAAA CAGGGCTTGT GTGAAACCCT GCAGTCCTGG 
AACAACAGAC TTCAAGAACT GTTCCAGCAA GAGAAAGGCA GAAAACGTCT ATCTGTTCCT 
CCTAGCCCAG GCAGACACAG ACAACCTACA ACAGATGAGA GCAAGACTAG TGCTTTGGAG 
TCTTCTCCTC GTAACCCTTC CCCAGTGGTC CCAAATGGAG ACAAAGAGGA CCGTCATCTC 
AATACATTTC CATCAAGTTC AGGGTCCTCG TCGTTACTAC AGTTACCATC TCCAGCTGAA 
CAACCTTCAG ATGTCATTAC AAGCGGACCA TCTTTCCCTG ATCAGGACTC TGTTAGCAAT 
CCAGAGGACA TGTTTGATGG ACACTTGCTT GGTTCCAATG ATAGTCAGGT AAAGGAAAAG 
TCCACCATGA AAACAATCCT GGCCAACCTG TTACCTGGAA ACAGCTACAA TCCCATCCCT 
TTTCCTTTTG ACCCAGACAA GCACTATCTG ATGTATGAGC ATGAGAGAGT TCCTATAGCC 
GTGTGTGAGA GAGAGCCCAG CTCCATAATT GCCTTTGCAC TCAGCTGCAA AGAGTATAAA 
ACTGCCCTTG AAGAGCTCAC AAAGACAACA GCAAAGACTG GAGGTGATGA CACTTCTCAG 
ACCATCAGTG CTGGAGAGAG CAGAGTGAAG AACAGTCCAG CCAAACCCAG TGACAACAGC 
ATGTCACAGC TGAGCCGCAG CAGCGTCGAC GCTGATCCAC TCAAGGAGCC TGAAAGTGCA 
GACAAACAGA AGAAGCAGAC AGGGAACCCA CATATTGAGC TGCAGTTCTC CGATGCAAAT 
GCTAAGTTCT ACTGCCGGAT CTACTATGCT GAAGAGTTCC ATAAAATGCG GGAGGAGATC 
ATGGAGAGCA CAGAGGATGA TTTTGTGCGA TCTCTTTCCC ACTGTGTCAA CTGGCAGGCG 
CGTGGTGGGA AATCTGGTGC AGTTTTCTAT GCCACTGAAG ATGATCGGTT TATTCTGAAG 
CAGATGCCCA GATTAGAGGT CCAGTCCTTT TTAGACTTTG CACCCCACTA CTTTACGTAC 
ATCACAGGAG CGGTTCACCT AAAGCGACCC ACAGCACTTG CTAAGATTCT GGGAGTGTAC 
CGCATCGGCT ACAAGAACTC CCAGAACAAC ACTGAGAAGA AGCTTGACCT GCTTGTGATG 
GAAAACCTTT TCTATGGACG AAAGATGGCA CAGGTGTTTG ACCTGAAAGG ATCGCTGAGA 
AACAGGAACG TGAAGACGGA CCAGGGAAAG GAGAGCTGTG AGGTGGTGCT CCTAGATGAG 
AACCTGCTCA AACTGGTGCA TGACAATCCC CTTTATATTC GCTCGCACTG CAAGGCCATC 
CTGCGTGCTG CCATCCACAG CGACGCGCTC TTCCTGTCCA GCCACCTCAT CATTGATTAC 
TCCCTGCTGG TGGGCCGAGA CGATTCCACA GATGAGCTAG TTGTGGGGAT CATAGATTAT 
ATCCGGACTT TCACTTGGGA TAAAAAGCTG GAGATGGTGG TCAAATCTAC TGGGATTCTT 
GGAGGTCAAG GTAAAATGCC CACAGTGGTG TCACCAGAGC TGTATCGAAC ACGTTTCTGT 
GAGGCCATGG ATAAATACTT CCTTATGGTC CCTGATCACT GGAGTGGACT TGGGCTCAAC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302996.1
CDS	156..6521
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304721.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304721.2

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAAGTAT GGAGCCCCAT 
GACCCGTTCA CCCCAGAAAC CGACATCCAT GCACCAATGG AAGTCTTGCT CTCCGAAGAT 
GGAGGTCAGC ATATATCCAT CAGTGATGCA TTCATCAAAG AGTCTTTGTT CAACCGGAGG 
GTTGAGGAGA AAGCTAAAGA GATGCTCTTC ACTCCTCTCG GCTGGCATCA CAGCTCTCTG 
GATCAGCTGC GGGAAGAGAA TGGGGAAAAG AAAGCGATGG AGCGGTTGCT GTCAGCAAAT 
CACAGCCACA TGATGGCGCT GCTGCAGCAA CTGCTGTACA GTGAATCACT GTCTCTGTCG 
TGGCGTGACA TCATCGTACC TGTGGTGAGG CAGGTGGTGC AGACGGTGCG ACCGGATGTG 
CGCAGCTGTG ACGATGATAT GGACATCCGT CAGTTTGTCC ACATCAAGAA GATTCCAGGA 
GGAAAGAAGT TTGACTCTGC TGTAGTGAAT GGTTTTGTTT GCACAAAGAA TATCGCACAC 
AAAAAGATGA ACTCTTACAT CAAAAACCCC AAGATCCTTC TTCTCAAATG CTCCATTGAG 
TATCTATATA GAGAGGAGAC CAAGTTCACC TGCATTGACC CAATTGTTTT ACAGGAGCGT 
GAGTTTCTTA AGAACTATGT TCAGAGGATT GTTGATGTCC GACCAAACTT AGTCCTGGTG 
GAGAAGACTG TATCGCGAAT TGCTCAGGAC ATGCTACTAG AGCACGGCAT TGCCATTGTG 
ATTAATGTCA AACCTCAAGT ACTGGACCGT GTGAGTCGAA TGACTCAGGG AGATTTAGTC 
ATTTCAATGG ATCAGCTTCT TACAAAACCT CGTCTGGGTA CCTGCCACAA GTTTTACTTG 
CACTCCTTCC AGCTACCAAA TAGTGAAGTG AAAACCCTGA TGTTTTTTGA GGGCTGTCCT 
CCTCAGCTGG GCTGCACTAT TAAGCTCCGC GGAGCCTCAG AGTATGAGCT GGCACGGGTA 
AAAGAGATCA TCATTTTTAT GGTGTGTGTG GCATACCACT CGCAGCTAGA GATCTCTTTC 
CTCATGGATG AGTTCGCTAT GCCTCCCAGT CTGGCTGAGA GTTCTTCTTT CCCATGTCTG 
CTGGAAAGCA CCACTTTACG GGAAGAGGAT GAAACTAGTG GGGAACGGGA AAGCCAAGAC 
AATGATGAGT CTACACTGGG AGATGACAAT CTCACACTTG TTCCAGGAGA TGACTTTGAG 
ACAGGATTTA CAGGGACTAT CAAAGCCCAC AGTCGAGAGC CTTCCACCTC TGAATCTTCT 
CATAAAGATG GAGAAAGCCC CAGAATAAAT AAAAATGCTT CAATTACCTC CTTCTCTGGA 
GGGGAGGAAG AAAATATAAA GACCTCCACG CCACTTTCCT CCTTCTCTTC CACCTTTCCT 
CAGCCGGTGA CACCACCTTT CCTGATATCA GACGTAAAAG AAATGTCACA GGAAGTGGTC 
AAGGGGTCAG GTGAAGGGGA GAAGAACAAA TATCTGGAGT CGACTCTGGT CCATCAGGAC 
AGCACAAGCT CTGAGACCTC CCTTCCCCCA ACTCGGCTCT TTAGAGACCC CTTACAGGAC 
GACACAGGCC TGTTTGTGAC AGAGCATGTA GCTTCTTCAG ATGACCGCCT CAAATCCATC 
TCTGCTTTAT TCAAACAGGA GCTAAAAGAC ATCATCCTGT GCATTTCACC CTTTATTACC 
TTTCGAGAGC CTTTCTTGCT TACCCCAGCT GGACTGCGGT GTCCTAGCCG GGATTATTTC 
CCTGAACAGG TTTACCTTTC ACCTCTGCTG AACAAGGATT CAAAAGAACT GGATGGACGC 
CGCAAGAGGC AGCTGCTGAA AGAGTCCAGC CCAGGTTCTG GTAGCCTGAC CAATGGTTCC 
GTTTCACACC AGAGAGCCAT TCAGATCTTG CCCTGTCACA AACTCACTAG TGCCCGAATA 
GCAGAACAGG TAGGCAGCAG TCAGCAGCTG GCACGCATGT TAGCGGACTA CCGTGCCCAA 
GGAGGACGTA TCCGGCAAAG AGAGGGAACA CAATTTCGTG AAGCCCCTCC CCCAAAGGCG 
CCAATAAAGG CAGATAGTGA AGAAGATAAA GGTGCAGGAG TGATTGACAT GACCTGGTCT 
ACTAAGTTGG ACTGTTTAAA TCCAATCAAC CATCAGAGGC TCTGTGTTCT GTTCAGTAGC 
TCTTCAGCAC AGTCTAATAA TGCCCCAAAC CCCTGCGTCA GTCCCTGGAT TGTTACAATG 
GAGTTTTATG GAAAGAATGA CTTGACTCTT GGTGTGTTTC TGGAGAGATA CTGTTTTAGA 
CCATCTTACC AGTGCCCCAG CATGTACTGT GAGACCCCCA TGGTTCACCA CATCCGGCGC 
TTTGTGCACG GTAAAGGCTG CGTCCAGATT GTACTGAAAG AGCTGGACTC GCCTGTTCCT 
GGATATCAGC ACACAATCCT CAACTACTCA TGGTGTCGCA TCTGCAAACA GGTGACTCCT 
GTGGTCCCTT TGTCTAATGA CTCTTGGTCC ATGTCCTTCG CCAAGTACCT TGAGCTTCGT 
TTCTATGGTC ATCAGTACAC CCGTCGGGCT AACGCTGAGC CTTGTGGCCA CTCTATCCAT 
AAAGATTATC ACCAGTACTT CTCTTACAAC CAGATGGTGG CATCCTTCAG CTACATTCCA 
GTGAGGCTGT TAGAGATCTG TCTGCCTCCC CCGAAGATCA TCATCAGGAA CCAAGGTCCA 
TCGAAATCCA GCTTGCAGCA GGATCTCAAA GACTTCTCCC AGAAGGTGGC TCAGGTGTAC 
CTGGCTATAG ATGACCGTCT TACCTCCTTA AAAACGGACA CATTCAGCAA GACCAGAGAG 
GAAAAAATGG AGGACATGTT TGCACAGAAA GATATGGAGG AATCAGAGCT TCGGGGCTGG 
ATAGAGAAGT TACAAGTGCG TCTGCAGACG AGTGTGATTG ACTCTTCACA ACAACTACAG 
GCTGTTCTGG AGTCAGTGGT GGTCAAAAAA CAGGGCTTGT GTGAAACCCT GCAGTCCTGG 
AACAACAGAC TTCAAGAACT GTTCCAGCAA GAGAAAGGCA GAAAACGTCT ATCTGTTCCT 
CCTAGCCCAG GCAGACACAG ACAACCTACA ACAGATGAGA GCAAGACTAG TGCTTTGGAG 
TCTTCTCCTC GTAACCCTTC CCCAGTGGTC CCAAATGGAG ACAAAGAGGA CCGTCATCTC 
AATACATTTC CATCAAGTTC AGGGTCCTCG TCGTTACTAC AGTTACCATC TCCAGCTGAA 
CAACCTTCAG ATGTCATTAC AAGCGGACCA TCTTTCCCTG ATCAGGACTC TGTTAGCAAT 
CCAGAGGACA TGTTTGATGG ACACTTGCTT GGTTCCAATG ATAGTCAGGT AAAGGAAAAG 
TCCACCATGA AAACAATCCT GGCCAACCTG TTACCTGGAA ACAGCTACAA TCCCATCCCT 
TTTCCTTTTG ACCCAGACAA GCACTATCTG ATGTATGAGC ATGAGAGAGT TCCTATAGCC 
GTGTGTGAGA GAGAGCCCAG CTCCATAATT GCCTTTGCAC TCAGCTGCAA AGAGTATAAA 
ACTGCCCTTG AAGAGCTCAC AAAGACAACA GCAAAGACTG GAGGTGATGA CACTTCTCAG 
ACCATCAGTG CTGGAGAGAG CAGAGTGAAG AACAGTCCAG CCAAACCCAG TGACAACAGC 
ATGTCACAGC TGAGCCGCAG CAGCGTCGAC GCTGATCCAC TCAAGGAGCC TGAAAGTGCA 
GACAAACAGA AGAAGCAGAC AGGGAACCCA CATATTGAGC TGCAGTTCTC CGATGCAAAT 
GCTAAGTTCT ACTGCCGGAT CTACTATGCT GAAGAGTTCC ATAAAATGCG GGAGGAGATC 
ATGGAGAGCA CAGAGGATGA TTTTGTGCGA TCTCTTTCCC ACTGTGTCAA CTGGCAGGCG 
CGTGGTGGGA AATCTGGTGC AGTTTTCTAT GCCACTGAAG ATGATCGGTT TATTCTGAAG 
CAGATGCCCA GATTAGAGGT CCAGTCCTTT TTAGACTTTG CACCCCACTA CTTTACGTAC 
ATCACAGGAG CGGTTCACCT AAAGCGACCC ACAGCACTTG CTAAGATTCT GGGAGTGTAC 
CGCATCGGCT ACAAGAACTC CCAGAACAAC ACTGAGAAGA AGCTTGACCT GCTTGTGATG 
GAAAACCTTT TCTATGGACG AAAGATGGCA CAGGTGTTTG ACCTGAAAGG ATCGCTGAGA 
AACAGGAACG TGAAGACGGA CCAGGGAAAG GAGAGCTGTG AGGTGGTGCT CCTAGATGAG 
AACCTGCTCA AACTGGTGCA TGACAATCCC CTTTATATTC GCTCGCACTG CAAGGCCATC 
CTGCGTGCTG CCATCCACAG CGACGCGCTC TTCCTGTCCA GCCACCTCAT CATTGATTAC 
TCCCTGCTGG TGGGCCGAGA CGATTCCACA GATGAGCTAG TTGTGGGGAT CATAGATTAT 
ATCCGGACTT TCACTTGGGA TAAAAAGCTG GAGATGGTGG TCAAATCTAC TGGGATTCTT 
GGAGGTCAAG GTAAAATGCC CACAGTGGTG TCACCAGAGC TGTATCGAAC ACGTTTCTGT 
GAGGCCATGG ATAAATACTT CCTTATGGTC CCTGATCACT GGAGTGGACT TGGGCTCAAC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304722.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302997.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.

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CloneID	ODa40123
Clone ID Related Accession (Same CDS sequence)	XM_009304722.2 , XM_009304722.3
Accession Version	XM_009304722.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAAGTATGG AGCCCCATGA CCCGTTCACC 
CCAGAAACCG ACATCCATGC ACCAATGGAA GTCTTGCTCT CCGAAGATGG AGGTCAGCAT 
ATATCCATCA GTGATGCATT CATCAAAGAG TCTTTGTTCA ACCGGAGGGT TGAGGAGAAA 
GCTAAAGAGA TGCTCTTCAC TCCTCTCGGC TGGCATCACA GCTCTCTGGA TCAGCTGCGG 
GAAGAGAATG GGGAAAAGAA AGCGATGGAG CGGTTGCTGT CAGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAACT GCTGTACAGT GAATCACTGT CTCTGTCGTG GCGTGACATC 
ATCGTACCTG TGGTGAGGCA GGTGGTGCAG ACGGTGCGAC CGGATGTGCG CAGCTGTGAC 
GATGATATGG ACATCCGTCA GTTTGTCCAC ATCAAGAAGA TTCCAGGAGG AAAGAAGTTT 
GACTCTGCTG TAGTGAATGG TTTTGTTTGC ACAAAGAATA TCGCACACAA AAAGATGAAC 
TCTTACATCA AAAACCCCAA GATCCTTCTT CTCAAATGCT CCATTGAGTA TCTATATAGA 
GAGGAGACCA AGTTCACCTG CATTGACCCA ATTGTTTTAC AGGAGCGTGA GTTTCTTAAG 
AACTATGTTC AGAGGATTGT TGATGTCCGA CCAAACTTAG TCCTGGTGGA GAAGACTGTA 
TCGCGAATTG CTCAGGACAT GCTACTAGAG CACGGCATTG CCATTGTGAT TAATGTCAAA 
CCTCAAGTAC TGGACCGTGT GAGTCGAATG ACTCAGGGAG ATTTAGTCAT TTCAATGGAT 
CAGCTTCTTA CAAAACCTCG TCTGGGTACC TGCCACAAGT TTTACTTGCA CTCCTTCCAG 
CTACCAAATA GTGAAGTGAA AACCCTGATG TTTTTTGAGG GCTGTCCTCC TCAGCTGGGC 
TGCACTATTA AGCTCCGCGG AGCCTCAGAG TATGAGCTGG CACGGGTAAA AGAGATCATC 
ATTTTTATGG TGTGTGTGGC ATACCACTCG CAGCTAGAGA TCTCTTTCCT CATGGATGAG 
TTCGCTATGC CTCCCAGTCT GGCTGAGAGT TCTTCTTTCC CATGTCTGCT GGAAAGCACC 
ACTTTACGGG AAGAGGATGA AACTAGTGGG GAACGGGAAA GCCAAGACAA TGATGAGTCT 
ACACTGGGAG ATGACAATCT CACACTTGTT CCAGGAGATG ACTTTGAGAC AGGATTTACA 
GGGACTATCA AAGCCCACAG TCGAGAGCCT TCCACCTCTG AATCTTCTCA TAAAGATGGA 
GAAAGCCCCA GAATAAATAA AAATGCTTCA ATTACCTCCT TCTCTGGAGG GGAGGAAGAA 
AATATAAAGA CCTCCACGCC ACTTTCCTCC TTCTCTTCCA CCTTTCCTCA GCCGGTGACA 
CCACCTTTCC TGATATCAGA CGTAAAAGAA ATGTCACAGG AAGTGGTCAA GGGGTCAGGT 
GAAGGGGAGA AGAACAAATA TCTGGAGTCG ACTCTGGTCC ATCAGGACAG CACAAGCTCT 
GAGACCTCCC TTCCCCCAAC TCGGCTCTTT AGAGACCCCT TACAGGACGA CACAGGCCTG 
TTTGTGACAG AGCATGTAGC TTCTTCAGAT GACCGCCTCA AATCCATCTC TGCTTTATTC 
AAACAGGAGC TAAAAGACAT CATCCTGTGC ATTTCACCCT TTATTACCTT TCGAGAGCCT 
TTCTTGCTTA CCCCAGCTGG ACTGCGGTGT CCTAGCCGGG ATTATTTCCC TGAACAGGTT 
TACCTTTCAC CTCTGCTGAA CAAGGATTCA AAAGAACTGG ATGGACGCCG CAAGAGGCAG 
CTGCTGAAAG AGTCCAGCCC AGGTTCTGGT AGCCTGACCA ATGGTTCCGT TTCACACCAG 
AGAGCCATTC AGATCTTGCC CTGTCACAAA CTCACTAGTG CCCGAATAGC AGAACAGGTA 
GGCAGCAGTC AGCAGCTGGC ACGCATGTTA GCGGACTACC GTGCCCAAGG AGGACGTATC 
CGGCAAAGAG AGGGAACACA ATTTCGTGAA GCCCCTCCCC CAAAGGCGCC AATAAAGGCA 
GATAGTGAAG AAGATAAAGG TGCAGGAGTG ATTGACATGA CCTGGTCTAC TAAGTTGGAC 
TGTTTAAATC CAATCAACCA TCAGAGGCTC TGTGTTCTGT TCAGTAGCTC TTCAGCACAG 
TCTAATAATG CCCCAAACCC CTGCGTCAGT CCCTGGATTG TTACAATGGA GTTTTATGGA 
AAGAATGACT TGACTCTTGG TGTGTTTCTG GAGAGATACT GTTTTAGACC ATCTTACCAG 
TGCCCCAGCA TGTACTGTGA GACCCCCATG GTTCACCACA TCCGGCGCTT TGTGCACGGT 
AAAGGCTGCG TCCAGATTGT ACTGAAAGAG CTGGACTCGC CTGTTCCTGG ATATCAGCAC 
ACAATCCTCA ACTACTCATG GTGTCGCATC TGCAAACAGG TGACTCCTGT GGTCCCTTTG 
TCTAATGACT CTTGGTCCAT GTCCTTCGCC AAGTACCTTG AGCTTCGTTT CTATGGTCAT 
CAGTACACCC GTCGGGCTAA CGCTGAGCCT TGTGGCCACT CTATCCATAA AGATTATCAC 
CAGTACTTCT CTTACAACCA GATGGTGGCA TCCTTCAGCT ACATTCCAGT GAGGCTGTTA 
GAGATCTGTC TGCCTCCCCC GAAGATCATC ATCAGGAACC AAGGTCCATC GAAATCCAGC 
TTGCAGCAGG ATCTCAAAGA CTTCTCCCAG AAGGTGGCTC AGGTGTACCT GGCTATAGAT 
GACCGTCTTA CCTCCTTAAA AACGGACACA TTCAGCAAGA CCAGAGAGGA AAAAATGGAG 
GACATGTTTG CACAGAAAGA TATGGAGGAA TCAGAGCTTC GGGGCTGGAT AGAGAAGTTA 
CAAGTGCGTC TGCAGACGAG TGTGATTGAC TCTTCACAAC AACTACAGGC TGTTCTGGAG 
TCAGTGGTGG TCAAAAAACA GGGCTTGTGT GAAACCCTGC AGTCCTGGAA CAACAGACTT 
CAAGAACTGT TCCAGCAAGA GAAAGGCAGA AAACGTCTAT CTGTTCCTCC TAGCCCAGGC 
AGACACAGAC AACCTACAAC AGATGAGAGC AAGACTAGTG CTTTGGAGTC TTCTCCTCGT 
AACCCTTCCC CAGTGGTCCC AAATGGAGAC AAAGAGGACC GTCATCTCAA TACATTTCCA 
TCAAGTTCAG GGTCCTCGTC GTTACTACAG TTACCATCTC CAGCTGAACA ACCTTCAGAT 
GTCATTACAA GCGGACCATC TTTCCCTGAT CAGGACTCTG TTAGCAATCC AGAGGACATG 
TTTGATGGAC ACTTGCTTGG TTCCAATGAT AGTCAGGTAA AGGAAAAGTC CACCATGAAA 
ACAATCCTGG CCAACCTGTT ACCTGGAAAC AGCTACAATC CCATCCCTTT TCCTTTTGAC 
CCAGACAAGC ACTATCTGAT GTATGAGCAT GAGAGAGTTC CTATAGCCGT GTGTGAGAGA 
GAGCCCAGCT CCATAATTGC CTTTGCACTC AGCTGCAAAG AGTATAAAAC TGCCCTTGAA 
GAGCTCACAA AGACAACAGC AAAGACTGGA GGTGATGACA CTTCTCAGAC CATCAGTGCT 
GGAGAGAGCA GAGTGAAGAA CAGTCCAGCC AAACCCAGTG ACAACAGCAT GTCACAGCTG 
AGCCGCAGCA GCGTCGACGC TGATCCACTC AAGGAGCCTG AAAGTGCAGA CAAACAGAAG 
AAGCAGACAG GGAACCCACA TATTGAGCTG CAGTTCTCCG ATGCAAATGC TAAGTTCTAC 
TGCCGGATCT ACTATGCTGA AGAGTTCCAT AAAATGCGGG AGGAGATCAT GGAGAGCACA 
GAGGATGATT TTGTGCGATC TCTTTCCCAC TGTGTCAACT GGCAGGCGCG TGGTGGGAAA 
TCTGGTGCAG TTTTCTATGC CACTGAAGAT GATCGGTTTA TTCTGAAGCA GATGCCCAGA 
TTAGAGGTCC AGTCCTTTTT AGACTTTGCA CCCCACTACT TTACGTACAT CACAGGAGCG 
GTTCACCTAA AGCGACCCAC AGCACTTGCT AAGATTCTGG GAGTGTACCG CATCGGCTAC 
AAGAACTCCC AGAACAACAC TGAGAAGAAG CTTGACCTGC TTGTGATGGA AAACCTTTTC 
TATGGACGAA AGATGGCACA GGTGTTTGAC CTGAAAGGAT CGCTGAGAAA CAGGAACGTG 
AAGACGGACC AGGGAAAGGA GAGCTGTGAG GTGGTGCTCC TAGATGAGAA CCTGCTCAAA 
CTGGTGCATG ACAATCCCCT TTATATTCGC TCGCACTGCA AGGCCATCCT GCGTGCTGCC 
ATCCACAGCG ACGCGCTCTT CCTGTCCAGC CACCTCATCA TTGATTACTC CCTGCTGGTG 
GGCCGAGACG ATTCCACAGA TGAGCTAGTT GTGGGGATCA TAGATTATAT CCGGACTTTC 
ACTTGGGATA AAAAGCTGGA GATGGTGGTC AAATCTACTG GGATTCTTGG AGGTCAAGGT 
AAAATGCCCA CAGTGGTGTC ACCAGAGCTG TATCGAACAC GTTTCTGTGA GGCCATGGAT 
AAATACTTCC TTATGGTCCC TGATCACTGG AGTGGACTTG GGCTCAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302997.1
CDS	156..6509
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304722.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304722.2

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAAGTATGG AGCCCCATGA CCCGTTCACC 
CCAGAAACCG ACATCCATGC ACCAATGGAA GTCTTGCTCT CCGAAGATGG AGGTCAGCAT 
ATATCCATCA GTGATGCATT CATCAAAGAG TCTTTGTTCA ACCGGAGGGT TGAGGAGAAA 
GCTAAAGAGA TGCTCTTCAC TCCTCTCGGC TGGCATCACA GCTCTCTGGA TCAGCTGCGG 
GAAGAGAATG GGGAAAAGAA AGCGATGGAG CGGTTGCTGT CAGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAACT GCTGTACAGT GAATCACTGT CTCTGTCGTG GCGTGACATC 
ATCGTACCTG TGGTGAGGCA GGTGGTGCAG ACGGTGCGAC CGGATGTGCG CAGCTGTGAC 
GATGATATGG ACATCCGTCA GTTTGTCCAC ATCAAGAAGA TTCCAGGAGG AAAGAAGTTT 
GACTCTGCTG TAGTGAATGG TTTTGTTTGC ACAAAGAATA TCGCACACAA AAAGATGAAC 
TCTTACATCA AAAACCCCAA GATCCTTCTT CTCAAATGCT CCATTGAGTA TCTATATAGA 
GAGGAGACCA AGTTCACCTG CATTGACCCA ATTGTTTTAC AGGAGCGTGA GTTTCTTAAG 
AACTATGTTC AGAGGATTGT TGATGTCCGA CCAAACTTAG TCCTGGTGGA GAAGACTGTA 
TCGCGAATTG CTCAGGACAT GCTACTAGAG CACGGCATTG CCATTGTGAT TAATGTCAAA 
CCTCAAGTAC TGGACCGTGT GAGTCGAATG ACTCAGGGAG ATTTAGTCAT TTCAATGGAT 
CAGCTTCTTA CAAAACCTCG TCTGGGTACC TGCCACAAGT TTTACTTGCA CTCCTTCCAG 
CTACCAAATA GTGAAGTGAA AACCCTGATG TTTTTTGAGG GCTGTCCTCC TCAGCTGGGC 
TGCACTATTA AGCTCCGCGG AGCCTCAGAG TATGAGCTGG CACGGGTAAA AGAGATCATC 
ATTTTTATGG TGTGTGTGGC ATACCACTCG CAGCTAGAGA TCTCTTTCCT CATGGATGAG 
TTCGCTATGC CTCCCAGTCT GGCTGAGAGT TCTTCTTTCC CATGTCTGCT GGAAAGCACC 
ACTTTACGGG AAGAGGATGA AACTAGTGGG GAACGGGAAA GCCAAGACAA TGATGAGTCT 
ACACTGGGAG ATGACAATCT CACACTTGTT CCAGGAGATG ACTTTGAGAC AGGATTTACA 
GGGACTATCA AAGCCCACAG TCGAGAGCCT TCCACCTCTG AATCTTCTCA TAAAGATGGA 
GAAAGCCCCA GAATAAATAA AAATGCTTCA ATTACCTCCT TCTCTGGAGG GGAGGAAGAA 
AATATAAAGA CCTCCACGCC ACTTTCCTCC TTCTCTTCCA CCTTTCCTCA GCCGGTGACA 
CCACCTTTCC TGATATCAGA CGTAAAAGAA ATGTCACAGG AAGTGGTCAA GGGGTCAGGT 
GAAGGGGAGA AGAACAAATA TCTGGAGTCG ACTCTGGTCC ATCAGGACAG CACAAGCTCT 
GAGACCTCCC TTCCCCCAAC TCGGCTCTTT AGAGACCCCT TACAGGACGA CACAGGCCTG 
TTTGTGACAG AGCATGTAGC TTCTTCAGAT GACCGCCTCA AATCCATCTC TGCTTTATTC 
AAACAGGAGC TAAAAGACAT CATCCTGTGC ATTTCACCCT TTATTACCTT TCGAGAGCCT 
TTCTTGCTTA CCCCAGCTGG ACTGCGGTGT CCTAGCCGGG ATTATTTCCC TGAACAGGTT 
TACCTTTCAC CTCTGCTGAA CAAGGATTCA AAAGAACTGG ATGGACGCCG CAAGAGGCAG 
CTGCTGAAAG AGTCCAGCCC AGGTTCTGGT AGCCTGACCA ATGGTTCCGT TTCACACCAG 
AGAGCCATTC AGATCTTGCC CTGTCACAAA CTCACTAGTG CCCGAATAGC AGAACAGGTA 
GGCAGCAGTC AGCAGCTGGC ACGCATGTTA GCGGACTACC GTGCCCAAGG AGGACGTATC 
CGGCAAAGAG AGGGAACACA ATTTCGTGAA GCCCCTCCCC CAAAGGCGCC AATAAAGGCA 
GATAGTGAAG AAGATAAAGG TGCAGGAGTG ATTGACATGA CCTGGTCTAC TAAGTTGGAC 
TGTTTAAATC CAATCAACCA TCAGAGGCTC TGTGTTCTGT TCAGTAGCTC TTCAGCACAG 
TCTAATAATG CCCCAAACCC CTGCGTCAGT CCCTGGATTG TTACAATGGA GTTTTATGGA 
AAGAATGACT TGACTCTTGG TGTGTTTCTG GAGAGATACT GTTTTAGACC ATCTTACCAG 
TGCCCCAGCA TGTACTGTGA GACCCCCATG GTTCACCACA TCCGGCGCTT TGTGCACGGT 
AAAGGCTGCG TCCAGATTGT ACTGAAAGAG CTGGACTCGC CTGTTCCTGG ATATCAGCAC 
ACAATCCTCA ACTACTCATG GTGTCGCATC TGCAAACAGG TGACTCCTGT GGTCCCTTTG 
TCTAATGACT CTTGGTCCAT GTCCTTCGCC AAGTACCTTG AGCTTCGTTT CTATGGTCAT 
CAGTACACCC GTCGGGCTAA CGCTGAGCCT TGTGGCCACT CTATCCATAA AGATTATCAC 
CAGTACTTCT CTTACAACCA GATGGTGGCA TCCTTCAGCT ACATTCCAGT GAGGCTGTTA 
GAGATCTGTC TGCCTCCCCC GAAGATCATC ATCAGGAACC AAGGTCCATC GAAATCCAGC 
TTGCAGCAGG ATCTCAAAGA CTTCTCCCAG AAGGTGGCTC AGGTGTACCT GGCTATAGAT 
GACCGTCTTA CCTCCTTAAA AACGGACACA TTCAGCAAGA CCAGAGAGGA AAAAATGGAG 
GACATGTTTG CACAGAAAGA TATGGAGGAA TCAGAGCTTC GGGGCTGGAT AGAGAAGTTA 
CAAGTGCGTC TGCAGACGAG TGTGATTGAC TCTTCACAAC AACTACAGGC TGTTCTGGAG 
TCAGTGGTGG TCAAAAAACA GGGCTTGTGT GAAACCCTGC AGTCCTGGAA CAACAGACTT 
CAAGAACTGT TCCAGCAAGA GAAAGGCAGA AAACGTCTAT CTGTTCCTCC TAGCCCAGGC 
AGACACAGAC AACCTACAAC AGATGAGAGC AAGACTAGTG CTTTGGAGTC TTCTCCTCGT 
AACCCTTCCC CAGTGGTCCC AAATGGAGAC AAAGAGGACC GTCATCTCAA TACATTTCCA 
TCAAGTTCAG GGTCCTCGTC GTTACTACAG TTACCATCTC CAGCTGAACA ACCTTCAGAT 
GTCATTACAA GCGGACCATC TTTCCCTGAT CAGGACTCTG TTAGCAATCC AGAGGACATG 
TTTGATGGAC ACTTGCTTGG TTCCAATGAT AGTCAGGTAA AGGAAAAGTC CACCATGAAA 
ACAATCCTGG CCAACCTGTT ACCTGGAAAC AGCTACAATC CCATCCCTTT TCCTTTTGAC 
CCAGACAAGC ACTATCTGAT GTATGAGCAT GAGAGAGTTC CTATAGCCGT GTGTGAGAGA 
GAGCCCAGCT CCATAATTGC CTTTGCACTC AGCTGCAAAG AGTATAAAAC TGCCCTTGAA 
GAGCTCACAA AGACAACAGC AAAGACTGGA GGTGATGACA CTTCTCAGAC CATCAGTGCT 
GGAGAGAGCA GAGTGAAGAA CAGTCCAGCC AAACCCAGTG ACAACAGCAT GTCACAGCTG 
AGCCGCAGCA GCGTCGACGC TGATCCACTC AAGGAGCCTG AAAGTGCAGA CAAACAGAAG 
AAGCAGACAG GGAACCCACA TATTGAGCTG CAGTTCTCCG ATGCAAATGC TAAGTTCTAC 
TGCCGGATCT ACTATGCTGA AGAGTTCCAT AAAATGCGGG AGGAGATCAT GGAGAGCACA 
GAGGATGATT TTGTGCGATC TCTTTCCCAC TGTGTCAACT GGCAGGCGCG TGGTGGGAAA 
TCTGGTGCAG TTTTCTATGC CACTGAAGAT GATCGGTTTA TTCTGAAGCA GATGCCCAGA 
TTAGAGGTCC AGTCCTTTTT AGACTTTGCA CCCCACTACT TTACGTACAT CACAGGAGCG 
GTTCACCTAA AGCGACCCAC AGCACTTGCT AAGATTCTGG GAGTGTACCG CATCGGCTAC 
AAGAACTCCC AGAACAACAC TGAGAAGAAG CTTGACCTGC TTGTGATGGA AAACCTTTTC 
TATGGACGAA AGATGGCACA GGTGTTTGAC CTGAAAGGAT CGCTGAGAAA CAGGAACGTG 
AAGACGGACC AGGGAAAGGA GAGCTGTGAG GTGGTGCTCC TAGATGAGAA CCTGCTCAAA 
CTGGTGCATG ACAATCCCCT TTATATTCGC TCGCACTGCA AGGCCATCCT GCGTGCTGCC 
ATCCACAGCG ACGCGCTCTT CCTGTCCAGC CACCTCATCA TTGATTACTC CCTGCTGGTG 
GGCCGAGACG ATTCCACAGA TGAGCTAGTT GTGGGGATCA TAGATTATAT CCGGACTTTC 
ACTTGGGATA AAAAGCTGGA GATGGTGGTC AAATCTACTG GGATTCTTGG AGGTCAAGGT 
AAAATGCCCA CAGTGGTGTC ACCAGAGCTG TATCGAACAC GTTTCTGTGA GGCCATGGAT 
AAATACTTCC TTATGGTCCC TGATCACTGG AGTGGACTTG GGCTCAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304722.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302997.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.

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CloneID	ODa40123
Clone ID Related Accession (Same CDS sequence)	XM_009304722.2 , XM_009304722.3
Accession Version	XM_009304722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAAGTATGG AGCCCCATGA CCCGTTCACC 
CCAGAAACCG ACATCCATGC ACCAATGGAA GTCTTGCTCT CCGAAGATGG AGGTCAGCAT 
ATATCCATCA GTGATGCATT CATCAAAGAG TCTTTGTTCA ACCGGAGGGT TGAGGAGAAA 
GCTAAAGAGA TGCTCTTCAC TCCTCTCGGC TGGCATCACA GCTCTCTGGA TCAGCTGCGG 
GAAGAGAATG GGGAAAAGAA AGCGATGGAG CGGTTGCTGT CAGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAACT GCTGTACAGT GAATCACTGT CTCTGTCGTG GCGTGACATC 
ATCGTACCTG TGGTGAGGCA GGTGGTGCAG ACGGTGCGAC CGGATGTGCG CAGCTGTGAC 
GATGATATGG ACATCCGTCA GTTTGTCCAC ATCAAGAAGA TTCCAGGAGG AAAGAAGTTT 
GACTCTGCTG TAGTGAATGG TTTTGTTTGC ACAAAGAATA TCGCACACAA AAAGATGAAC 
TCTTACATCA AAAACCCCAA GATCCTTCTT CTCAAATGCT CCATTGAGTA TCTATATAGA 
GAGGAGACCA AGTTCACCTG CATTGACCCA ATTGTTTTAC AGGAGCGTGA GTTTCTTAAG 
AACTATGTTC AGAGGATTGT TGATGTCCGA CCAAACTTAG TCCTGGTGGA GAAGACTGTA 
TCGCGAATTG CTCAGGACAT GCTACTAGAG CACGGCATTG CCATTGTGAT TAATGTCAAA 
CCTCAAGTAC TGGACCGTGT GAGTCGAATG ACTCAGGGAG ATTTAGTCAT TTCAATGGAT 
CAGCTTCTTA CAAAACCTCG TCTGGGTACC TGCCACAAGT TTTACTTGCA CTCCTTCCAG 
CTACCAAATA GTGAAGTGAA AACCCTGATG TTTTTTGAGG GCTGTCCTCC TCAGCTGGGC 
TGCACTATTA AGCTCCGCGG AGCCTCAGAG TATGAGCTGG CACGGGTAAA AGAGATCATC 
ATTTTTATGG TGTGTGTGGC ATACCACTCG CAGCTAGAGA TCTCTTTCCT CATGGATGAG 
TTCGCTATGC CTCCCAGTCT GGCTGAGAGT TCTTCTTTCC CATGTCTGCT GGAAAGCACC 
ACTTTACGGG AAGAGGATGA AACTAGTGGG GAACGGGAAA GCCAAGACAA TGATGAGTCT 
ACACTGGGAG ATGACAATCT CACACTTGTT CCAGGAGATG ACTTTGAGAC AGGATTTACA 
GGGACTATCA AAGCCCACAG TCGAGAGCCT TCCACCTCTG AATCTTCTCA TAAAGATGGA 
GAAAGCCCCA GAATAAATAA AAATGCTTCA ATTACCTCCT TCTCTGGAGG GGAGGAAGAA 
AATATAAAGA CCTCCACGCC ACTTTCCTCC TTCTCTTCCA CCTTTCCTCA GCCGGTGACA 
CCACCTTTCC TGATATCAGA CGTAAAAGAA ATGTCACAGG AAGTGGTCAA GGGGTCAGGT 
GAAGGGGAGA AGAACAAATA TCTGGAGTCG ACTCTGGTCC ATCAGGACAG CACAAGCTCT 
GAGACCTCCC TTCCCCCAAC TCGGCTCTTT AGAGACCCCT TACAGGACGA CACAGGCCTG 
TTTGTGACAG AGCATGTAGC TTCTTCAGAT GACCGCCTCA AATCCATCTC TGCTTTATTC 
AAACAGGAGC TAAAAGACAT CATCCTGTGC ATTTCACCCT TTATTACCTT TCGAGAGCCT 
TTCTTGCTTA CCCCAGCTGG ACTGCGGTGT CCTAGCCGGG ATTATTTCCC TGAACAGGTT 
TACCTTTCAC CTCTGCTGAA CAAGGATTCA AAAGAACTGG ATGGACGCCG CAAGAGGCAG 
CTGCTGAAAG AGTCCAGCCC AGGTTCTGGT AGCCTGACCA ATGGTTCCGT TTCACACCAG 
AGAGCCATTC AGATCTTGCC CTGTCACAAA CTCACTAGTG CCCGAATAGC AGAACAGGTA 
GGCAGCAGTC AGCAGCTGGC ACGCATGTTA GCGGACTACC GTGCCCAAGG AGGACGTATC 
CGGCAAAGAG AGGGAACACA ATTTCGTGAA GCCCCTCCCC CAAAGGCGCC AATAAAGGCA 
GATAGTGAAG AAGATAAAGG TGCAGGAGTG ATTGACATGA CCTGGTCTAC TAAGTTGGAC 
TGTTTAAATC CAATCAACCA TCAGAGGCTC TGTGTTCTGT TCAGTAGCTC TTCAGCACAG 
TCTAATAATG CCCCAAACCC CTGCGTCAGT CCCTGGATTG TTACAATGGA GTTTTATGGA 
AAGAATGACT TGACTCTTGG TGTGTTTCTG GAGAGATACT GTTTTAGACC ATCTTACCAG 
TGCCCCAGCA TGTACTGTGA GACCCCCATG GTTCACCACA TCCGGCGCTT TGTGCACGGT 
AAAGGCTGCG TCCAGATTGT ACTGAAAGAG CTGGACTCGC CTGTTCCTGG ATATCAGCAC 
ACAATCCTCA ACTACTCATG GTGTCGCATC TGCAAACAGG TGACTCCTGT GGTCCCTTTG 
TCTAATGACT CTTGGTCCAT GTCCTTCGCC AAGTACCTTG AGCTTCGTTT CTATGGTCAT 
CAGTACACCC GTCGGGCTAA CGCTGAGCCT TGTGGCCACT CTATCCATAA AGATTATCAC 
CAGTACTTCT CTTACAACCA GATGGTGGCA TCCTTCAGCT ACATTCCAGT GAGGCTGTTA 
GAGATCTGTC TGCCTCCCCC GAAGATCATC ATCAGGAACC AAGGTCCATC GAAATCCAGC 
TTGCAGCAGG ATCTCAAAGA CTTCTCCCAG AAGGTGGCTC AGGTGTACCT GGCTATAGAT 
GACCGTCTTA CCTCCTTAAA AACGGACACA TTCAGCAAGA CCAGAGAGGA AAAAATGGAG 
GACATGTTTG CACAGAAAGA TATGGAGGAA TCAGAGCTTC GGGGCTGGAT AGAGAAGTTA 
CAAGTGCGTC TGCAGACGAG TGTGATTGAC TCTTCACAAC AACTACAGGC TGTTCTGGAG 
TCAGTGGTGG TCAAAAAACA GGGCTTGTGT GAAACCCTGC AGTCCTGGAA CAACAGACTT 
CAAGAACTGT TCCAGCAAGA GAAAGGCAGA AAACGTCTAT CTGTTCCTCC TAGCCCAGGC 
AGACACAGAC AACCTACAAC AGATGAGAGC AAGACTAGTG CTTTGGAGTC TTCTCCTCGT 
AACCCTTCCC CAGTGGTCCC AAATGGAGAC AAAGAGGACC GTCATCTCAA TACATTTCCA 
TCAAGTTCAG GGTCCTCGTC GTTACTACAG TTACCATCTC CAGCTGAACA ACCTTCAGAT 
GTCATTACAA GCGGACCATC TTTCCCTGAT CAGGACTCTG TTAGCAATCC AGAGGACATG 
TTTGATGGAC ACTTGCTTGG TTCCAATGAT AGTCAGGTAA AGGAAAAGTC CACCATGAAA 
ACAATCCTGG CCAACCTGTT ACCTGGAAAC AGCTACAATC CCATCCCTTT TCCTTTTGAC 
CCAGACAAGC ACTATCTGAT GTATGAGCAT GAGAGAGTTC CTATAGCCGT GTGTGAGAGA 
GAGCCCAGCT CCATAATTGC CTTTGCACTC AGCTGCAAAG AGTATAAAAC TGCCCTTGAA 
GAGCTCACAA AGACAACAGC AAAGACTGGA GGTGATGACA CTTCTCAGAC CATCAGTGCT 
GGAGAGAGCA GAGTGAAGAA CAGTCCAGCC AAACCCAGTG ACAACAGCAT GTCACAGCTG 
AGCCGCAGCA GCGTCGACGC TGATCCACTC AAGGAGCCTG AAAGTGCAGA CAAACAGAAG 
AAGCAGACAG GGAACCCACA TATTGAGCTG CAGTTCTCCG ATGCAAATGC TAAGTTCTAC 
TGCCGGATCT ACTATGCTGA AGAGTTCCAT AAAATGCGGG AGGAGATCAT GGAGAGCACA 
GAGGATGATT TTGTGCGATC TCTTTCCCAC TGTGTCAACT GGCAGGCGCG TGGTGGGAAA 
TCTGGTGCAG TTTTCTATGC CACTGAAGAT GATCGGTTTA TTCTGAAGCA GATGCCCAGA 
TTAGAGGTCC AGTCCTTTTT AGACTTTGCA CCCCACTACT TTACGTACAT CACAGGAGCG 
GTTCACCTAA AGCGACCCAC AGCACTTGCT AAGATTCTGG GAGTGTACCG CATCGGCTAC 
AAGAACTCCC AGAACAACAC TGAGAAGAAG CTTGACCTGC TTGTGATGGA AAACCTTTTC 
TATGGACGAA AGATGGCACA GGTGTTTGAC CTGAAAGGAT CGCTGAGAAA CAGGAACGTG 
AAGACGGACC AGGGAAAGGA GAGCTGTGAG GTGGTGCTCC TAGATGAGAA CCTGCTCAAA 
CTGGTGCATG ACAATCCCCT TTATATTCGC TCGCACTGCA AGGCCATCCT GCGTGCTGCC 
ATCCACAGCG ACGCGCTCTT CCTGTCCAGC CACCTCATCA TTGATTACTC CCTGCTGGTG 
GGCCGAGACG ATTCCACAGA TGAGCTAGTT GTGGGGATCA TAGATTATAT CCGGACTTTC 
ACTTGGGATA AAAAGCTGGA GATGGTGGTC AAATCTACTG GGATTCTTGG AGGTCAAGGT 
AAAATGCCCA CAGTGGTGTC ACCAGAGCTG TATCGAACAC GTTTCTGTGA GGCCATGGAT 
AAATACTTCC TTATGGTCCC TGATCACTGG AGTGGACTTG GGCTCAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302997.1
CDS	164..6517
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304722.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304722.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAAGTATGG AGCCCCATGA CCCGTTCACC 
CCAGAAACCG ACATCCATGC ACCAATGGAA GTCTTGCTCT CCGAAGATGG AGGTCAGCAT 
ATATCCATCA GTGATGCATT CATCAAAGAG TCTTTGTTCA ACCGGAGGGT TGAGGAGAAA 
GCTAAAGAGA TGCTCTTCAC TCCTCTCGGC TGGCATCACA GCTCTCTGGA TCAGCTGCGG 
GAAGAGAATG GGGAAAAGAA AGCGATGGAG CGGTTGCTGT CAGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAACT GCTGTACAGT GAATCACTGT CTCTGTCGTG GCGTGACATC 
ATCGTACCTG TGGTGAGGCA GGTGGTGCAG ACGGTGCGAC CGGATGTGCG CAGCTGTGAC 
GATGATATGG ACATCCGTCA GTTTGTCCAC ATCAAGAAGA TTCCAGGAGG AAAGAAGTTT 
GACTCTGCTG TAGTGAATGG TTTTGTTTGC ACAAAGAATA TCGCACACAA AAAGATGAAC 
TCTTACATCA AAAACCCCAA GATCCTTCTT CTCAAATGCT CCATTGAGTA TCTATATAGA 
GAGGAGACCA AGTTCACCTG CATTGACCCA ATTGTTTTAC AGGAGCGTGA GTTTCTTAAG 
AACTATGTTC AGAGGATTGT TGATGTCCGA CCAAACTTAG TCCTGGTGGA GAAGACTGTA 
TCGCGAATTG CTCAGGACAT GCTACTAGAG CACGGCATTG CCATTGTGAT TAATGTCAAA 
CCTCAAGTAC TGGACCGTGT GAGTCGAATG ACTCAGGGAG ATTTAGTCAT TTCAATGGAT 
CAGCTTCTTA CAAAACCTCG TCTGGGTACC TGCCACAAGT TTTACTTGCA CTCCTTCCAG 
CTACCAAATA GTGAAGTGAA AACCCTGATG TTTTTTGAGG GCTGTCCTCC TCAGCTGGGC 
TGCACTATTA AGCTCCGCGG AGCCTCAGAG TATGAGCTGG CACGGGTAAA AGAGATCATC 
ATTTTTATGG TGTGTGTGGC ATACCACTCG CAGCTAGAGA TCTCTTTCCT CATGGATGAG 
TTCGCTATGC CTCCCAGTCT GGCTGAGAGT TCTTCTTTCC CATGTCTGCT GGAAAGCACC 
ACTTTACGGG AAGAGGATGA AACTAGTGGG GAACGGGAAA GCCAAGACAA TGATGAGTCT 
ACACTGGGAG ATGACAATCT CACACTTGTT CCAGGAGATG ACTTTGAGAC AGGATTTACA 
GGGACTATCA AAGCCCACAG TCGAGAGCCT TCCACCTCTG AATCTTCTCA TAAAGATGGA 
GAAAGCCCCA GAATAAATAA AAATGCTTCA ATTACCTCCT TCTCTGGAGG GGAGGAAGAA 
AATATAAAGA CCTCCACGCC ACTTTCCTCC TTCTCTTCCA CCTTTCCTCA GCCGGTGACA 
CCACCTTTCC TGATATCAGA CGTAAAAGAA ATGTCACAGG AAGTGGTCAA GGGGTCAGGT 
GAAGGGGAGA AGAACAAATA TCTGGAGTCG ACTCTGGTCC ATCAGGACAG CACAAGCTCT 
GAGACCTCCC TTCCCCCAAC TCGGCTCTTT AGAGACCCCT TACAGGACGA CACAGGCCTG 
TTTGTGACAG AGCATGTAGC TTCTTCAGAT GACCGCCTCA AATCCATCTC TGCTTTATTC 
AAACAGGAGC TAAAAGACAT CATCCTGTGC ATTTCACCCT TTATTACCTT TCGAGAGCCT 
TTCTTGCTTA CCCCAGCTGG ACTGCGGTGT CCTAGCCGGG ATTATTTCCC TGAACAGGTT 
TACCTTTCAC CTCTGCTGAA CAAGGATTCA AAAGAACTGG ATGGACGCCG CAAGAGGCAG 
CTGCTGAAAG AGTCCAGCCC AGGTTCTGGT AGCCTGACCA ATGGTTCCGT TTCACACCAG 
AGAGCCATTC AGATCTTGCC CTGTCACAAA CTCACTAGTG CCCGAATAGC AGAACAGGTA 
GGCAGCAGTC AGCAGCTGGC ACGCATGTTA GCGGACTACC GTGCCCAAGG AGGACGTATC 
CGGCAAAGAG AGGGAACACA ATTTCGTGAA GCCCCTCCCC CAAAGGCGCC AATAAAGGCA 
GATAGTGAAG AAGATAAAGG TGCAGGAGTG ATTGACATGA CCTGGTCTAC TAAGTTGGAC 
TGTTTAAATC CAATCAACCA TCAGAGGCTC TGTGTTCTGT TCAGTAGCTC TTCAGCACAG 
TCTAATAATG CCCCAAACCC CTGCGTCAGT CCCTGGATTG TTACAATGGA GTTTTATGGA 
AAGAATGACT TGACTCTTGG TGTGTTTCTG GAGAGATACT GTTTTAGACC ATCTTACCAG 
TGCCCCAGCA TGTACTGTGA GACCCCCATG GTTCACCACA TCCGGCGCTT TGTGCACGGT 
AAAGGCTGCG TCCAGATTGT ACTGAAAGAG CTGGACTCGC CTGTTCCTGG ATATCAGCAC 
ACAATCCTCA ACTACTCATG GTGTCGCATC TGCAAACAGG TGACTCCTGT GGTCCCTTTG 
TCTAATGACT CTTGGTCCAT GTCCTTCGCC AAGTACCTTG AGCTTCGTTT CTATGGTCAT 
CAGTACACCC GTCGGGCTAA CGCTGAGCCT TGTGGCCACT CTATCCATAA AGATTATCAC 
CAGTACTTCT CTTACAACCA GATGGTGGCA TCCTTCAGCT ACATTCCAGT GAGGCTGTTA 
GAGATCTGTC TGCCTCCCCC GAAGATCATC ATCAGGAACC AAGGTCCATC GAAATCCAGC 
TTGCAGCAGG ATCTCAAAGA CTTCTCCCAG AAGGTGGCTC AGGTGTACCT GGCTATAGAT 
GACCGTCTTA CCTCCTTAAA AACGGACACA TTCAGCAAGA CCAGAGAGGA AAAAATGGAG 
GACATGTTTG CACAGAAAGA TATGGAGGAA TCAGAGCTTC GGGGCTGGAT AGAGAAGTTA 
CAAGTGCGTC TGCAGACGAG TGTGATTGAC TCTTCACAAC AACTACAGGC TGTTCTGGAG 
TCAGTGGTGG TCAAAAAACA GGGCTTGTGT GAAACCCTGC AGTCCTGGAA CAACAGACTT 
CAAGAACTGT TCCAGCAAGA GAAAGGCAGA AAACGTCTAT CTGTTCCTCC TAGCCCAGGC 
AGACACAGAC AACCTACAAC AGATGAGAGC AAGACTAGTG CTTTGGAGTC TTCTCCTCGT 
AACCCTTCCC CAGTGGTCCC AAATGGAGAC AAAGAGGACC GTCATCTCAA TACATTTCCA 
TCAAGTTCAG GGTCCTCGTC GTTACTACAG TTACCATCTC CAGCTGAACA ACCTTCAGAT 
GTCATTACAA GCGGACCATC TTTCCCTGAT CAGGACTCTG TTAGCAATCC AGAGGACATG 
TTTGATGGAC ACTTGCTTGG TTCCAATGAT AGTCAGGTAA AGGAAAAGTC CACCATGAAA 
ACAATCCTGG CCAACCTGTT ACCTGGAAAC AGCTACAATC CCATCCCTTT TCCTTTTGAC 
CCAGACAAGC ACTATCTGAT GTATGAGCAT GAGAGAGTTC CTATAGCCGT GTGTGAGAGA 
GAGCCCAGCT CCATAATTGC CTTTGCACTC AGCTGCAAAG AGTATAAAAC TGCCCTTGAA 
GAGCTCACAA AGACAACAGC AAAGACTGGA GGTGATGACA CTTCTCAGAC CATCAGTGCT 
GGAGAGAGCA GAGTGAAGAA CAGTCCAGCC AAACCCAGTG ACAACAGCAT GTCACAGCTG 
AGCCGCAGCA GCGTCGACGC TGATCCACTC AAGGAGCCTG AAAGTGCAGA CAAACAGAAG 
AAGCAGACAG GGAACCCACA TATTGAGCTG CAGTTCTCCG ATGCAAATGC TAAGTTCTAC 
TGCCGGATCT ACTATGCTGA AGAGTTCCAT AAAATGCGGG AGGAGATCAT GGAGAGCACA 
GAGGATGATT TTGTGCGATC TCTTTCCCAC TGTGTCAACT GGCAGGCGCG TGGTGGGAAA 
TCTGGTGCAG TTTTCTATGC CACTGAAGAT GATCGGTTTA TTCTGAAGCA GATGCCCAGA 
TTAGAGGTCC AGTCCTTTTT AGACTTTGCA CCCCACTACT TTACGTACAT CACAGGAGCG 
GTTCACCTAA AGCGACCCAC AGCACTTGCT AAGATTCTGG GAGTGTACCG CATCGGCTAC 
AAGAACTCCC AGAACAACAC TGAGAAGAAG CTTGACCTGC TTGTGATGGA AAACCTTTTC 
TATGGACGAA AGATGGCACA GGTGTTTGAC CTGAAAGGAT CGCTGAGAAA CAGGAACGTG 
AAGACGGACC AGGGAAAGGA GAGCTGTGAG GTGGTGCTCC TAGATGAGAA CCTGCTCAAA 
CTGGTGCATG ACAATCCCCT TTATATTCGC TCGCACTGCA AGGCCATCCT GCGTGCTGCC 
ATCCACAGCG ACGCGCTCTT CCTGTCCAGC CACCTCATCA TTGATTACTC CCTGCTGGTG 
GGCCGAGACG ATTCCACAGA TGAGCTAGTT GTGGGGATCA TAGATTATAT CCGGACTTTC 
ACTTGGGATA AAAAGCTGGA GATGGTGGTC AAATCTACTG GGATTCTTGG AGGTCAAGGT 
AAAATGCCCA CAGTGGTGTC ACCAGAGCTG TATCGAACAC GTTTCTGTGA GGCCATGGAT 
AAATACTTCC TTATGGTCCC TGATCACTGG AGTGGACTTG GGCTCAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304723.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302998.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.

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CloneID	ODa40124
Clone ID Related Accession (Same CDS sequence)	XM_009304723.3 , XM_009304723.2
Accession Version	XM_009304723.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAG TATGGAGCCC CATGACCCGT TCACCCCAGA AACCGACATC 
CATGCACCAA TGGAAGTCTT GCTCTCCGAA GATGGAGGTC AGCATATATC CATCAGTGAT 
GCATTCATCA AAGAGTCTTT GTTCAACCGG AGGGTTGAGG AGAAAGCTAA AGAGATGCTC 
TTCACTCCTC TCGGCTGGCA TCACAGCTCT CTGGATCAGC TGCGGGAAGA GAATGGGGAA 
AAGAAAGCGA TGGAGCGGTT GCTGTCAGCA AATCACAGCC ACATGATGGC GCTGCTGCAG 
CAACTGCTGT ACAGTGAATC ACTGTCTCTG TCGTGGCGTG ACATCATCGT ACCTGTGGTG 
AGGCAGGTGG TGCAGACGGT GCGACCGGAT GTGCGCAGCT GTGACGATGA TATGGACATC 
CGTCAGTTTG TCCACATCAA GAAGATTCCA GGAGGAAAGA AGTTTGACTC TGCTGTAGTG 
AATGGTTTTG TTTGCACAAA GAATATCGCA CACAAAAAGA TGAACTCTTA CATCAAAAAC 
CCCAAGATCC TTCTTCTCAA ATGCTCCATT GAGTATCTAT ATAGAGAGGA GACCAAGTTC 
ACCTGCATTG ACCCAATTGT TTTACAGGAG CGTGAGTTTC TTAAGAACTA TGTTCAGAGG 
ATTGTTGATG TCCGACCAAA CTTAGTCCTG GTGGAGAAGA CTGTATCGCG AATTGCTCAG 
GACATGCTAC TAGAGCACGG CATTGCCATT GTGATTAATG TCAAACCTCA AGTACTGGAC 
CGTGTGAGTC GAATGACTCA GGGAGATTTA GTCATTTCAA TGGATCAGCT TCTTACAAAA 
CCTCGTCTGG GTACCTGCCA CAAGTTTTAC TTGCACTCCT TCCAGCTACC AAATAGTGAA 
GTGAAAACCC TGATGTTTTT TGAGGGCTGT CCTCCTCAGC TGGGCTGCAC TATTAAGCTC 
CGCGGAGCCT CAGAGTATGA GCTGGCACGG GTAAAAGAGA TCATCATTTT TATGGTGTGT 
GTGGCATACC ACTCGCAGCT AGAGATCTCT TTCCTCATGG ATGAGTTCGC TATGCCTCCC 
AGTCTGGCTG AGAGTTCTTC TTTCCCATGT CTGCTGGAAA GCACCACTTT ACGGGAAGAG 
GATGAAACTA GTGGGGAACG GGAAAGCCAA GACAATGATG AGTCTACACT GGGAGATGAC 
AATCTCACAC TTGTTCCAGG AGATGACTTT GAGACAGGAT TTACAGGGAC TATCAAAGCC 
CACAGTCGAG AGCCTTCCAC CTCTGAATCT TCTCATAAAG ATGGAGAAAG CCCCAGAATA 
AATAAAAATG CTTCAATTAC CTCCTTCTCT GGAGGGGAGG AAGAAAATAT AAAGACCTCC 
ACGCCACTTT CCTCCTTCTC TTCCACCTTT CCTCAGCCGG TGACACCACC TTTCCTGATA 
TCAGACGTAA AAGAAATGTC ACAGGAAGTG GTCAAGGGGT CAGGTGAAGG GGAGAAGAAC 
AAATATCTGG AGTCGACTCT GGTCCATCAG GACAGCACAA GCTCTGAGAC CTCCCTTCCC 
CCAACTCGGC TCTTTAGAGA CCCCTTACAG GACGACACAG GCCTGTTTGT GACAGAGCAT 
GTAGCTTCTT CAGATGACCG CCTCAAATCC ATCTCTGCTT TATTCAAACA GGAGCTAAAA 
GACATCATCC TGTGCATTTC ACCCTTTATT ACCTTTCGAG AGCCTTTCTT GCTTACCCCA 
GCTGGACTGC GGTGTCCTAG CCGGGATTAT TTCCCTGAAC AGGTTTACCT TTCACCTCTG 
CTGAACAAGG ATTCAAAAGA ACTGGATGGA CGCCGCAAGA GGCAGCTGCT GAAAGAGTCC 
AGCCCAGGTT CTGGTAGCCT GACCAATGGT TCCGTTTCAC ACCAGAGAGC CATTCAGATC 
TTGCCCTGTC ACAAACTCAC TAGTGCCCGA ATAGCAGAAC AGGTAGGCAG CAGTCAGCAG 
CTGGCACGCA TGTTAGCGGA CTACCGTGCC CAAGGAGGAC GTATCCGGCA AAGAGAGGGA 
ACACAATTTC GTGAAGCCCC TCCCCCAAAG GCGCCAATAA AGGCAGATAG TGAAGAAGAT 
AAAGGTGCAG GAGTGATTGA CATGACCTGG TCTACTAAGT TGGACTGTTT AAATCCAATC 
AACCATCAGA GGCTCTGTGT TCTGTTCAGT AGCTCTTCAG CACAGTCTAA TAATGCCCCA 
AACCCCTGCG TCAGTCCCTG GATTGTTACA ATGGAGTTTT ATGGAAAGAA TGACTTGACT 
CTTGGTGTGT TTCTGGAGAG ATACTGTTTT AGACCATCTT ACCAGTGCCC CAGCATGTAC 
TGTGAGACCC CCATGGTTCA CCACATCCGG CGCTTTGTGC ACGGTAAAGG CTGCGTCCAG 
ATTGTACTGA AAGAGCTGGA CTCGCCTGTT CCTGGATATC AGCACACAAT CCTCAACTAC 
TCATGGTGTC GCATCTGCAA ACAGGTGACT CCTGTGGTCC CTTTGTCTAA TGACTCTTGG 
TCCATGTCCT TCGCCAAGTA CCTTGAGCTT CGTTTCTATG GTCATCAGTA CACCCGTCGG 
GCTAACGCTG AGCCTTGTGG CCACTCTATC CATAAAGATT ATCACCAGTA CTTCTCTTAC 
AACCAGATGG TGGCATCCTT CAGCTACATT CCAGTGAGGC TGTTAGAGAT CTGTCTGCCT 
CCCCCGAAGA TCATCATCAG GAACCAAGGT CCATCGAAAT CCAGCTTGCA GCAGGATCTC 
AAAGACTTCT CCCAGAAGGT GGCTCAGGTG TACCTGGCTA TAGATGACCG TCTTACCTCC 
TTAAAAACGG ACACATTCAG CAAGACCAGA GAGGAAAAAA TGGAGGACAT GTTTGCACAG 
AAAGATATGG AGGAATCAGA GCTTCGGGGC TGGATAGAGA AGTTACAAGT GCGTCTGCAG 
ACGAGTGTGA TTGACTCTTC ACAACAACTA CAGGCTGTTC TGGAGTCAGT GGTGGTCAAA 
AAACAGGGCT TGTGTGAAAC CCTGCAGTCC TGGAACAACA GACTTCAAGA ACTGTTCCAG 
CAAGAGAAAG GCAGAAAACG TCTATCTGTT CCTCCTAGCC CAGGCAGACA CAGACAACCT 
ACAACAGATG AGAGCAAGAC TAGTGCTTTG GAGTCTTCTC CTCGTAACCC TTCCCCAGTG 
GTCCCAAATG GAGACAAAGA GGACCGTCAT CTCAATACAT TTCCATCAAG TTCAGGGTCC 
TCGTCGTTAC TACAGTTACC ATCTCCAGCT GAACAACCTT CAGATGTCAT TACAAGCGGA 
CCATCTTTCC CTGATCAGGA CTCTGTTAGC AATCCAGAGG ACATGTTTGA TGGACACTTG 
CTTGGTTCCA ATGATAGTCA GGTAAAGGAA AAGTCCACCA TGAAAACAAT CCTGGCCAAC 
CTGTTACCTG GAAACAGCTA CAATCCCATC CCTTTTCCTT TTGACCCAGA CAAGCACTAT 
CTGATGTATG AGCATGAGAG AGTTCCTATA GCCGTGTGTG AGAGAGAGCC CAGCTCCATA 
ATTGCCTTTG CACTCAGCTG CAAAGAGTAT AAAACTGCCC TTGAAGAGCT CACAAAGACA 
ACAGCAAAGA CTGGAGGTGA TGACACTTCT CAGACCATCA GTGCTGGAGA GAGCAGAGTG 
AAGAACAGTC CAGCCAAACC CAGTGACAAC AGCATGTCAC AGCTGAGCCG CAGCAGCGTC 
GACGCTGATC CACTCAAGGA GCCTGAAAGT GCAGACAAAC AGAAGAAGCA GACAGGGAAC 
CCACATATTG AGCTGCAGTT CTCCGATGCA AATGCTAAGT TCTACTGCCG GATCTACTAT 
GCTGAAGAGT TCCATAAAAT GCGGGAGGAG ATCATGGAGA GCACAGAGGA TGATTTTGTG 
CGATCTCTTT CCCACTGTGT CAACTGGCAG GCGCGTGGTG GGAAATCTGG TGCAGTTTTC 
TATGCCACTG AAGATGATCG GTTTATTCTG AAGCAGATGC CCAGATTAGA GGTCCAGTCC 
TTTTTAGACT TTGCACCCCA CTACTTTACG TACATCACAG GAGCGGTTCA CCTAAAGCGA 
CCCACAGCAC TTGCTAAGAT TCTGGGAGTG TACCGCATCG GCTACAAGAA CTCCCAGAAC 
AACACTGAGA AGAAGCTTGA CCTGCTTGTG ATGGAAAACC TTTTCTATGG ACGAAAGATG 
GCACAGGTGT TTGACCTGAA AGGATCGCTG AGAAACAGGA ACGTGAAGAC GGACCAGGGA 
AAGGAGAGCT GTGAGGTGGT GCTCCTAGAT GAGAACCTGC TCAAACTGGT GCATGACAAT 
CCCCTTTATA TTCGCTCGCA CTGCAAGGCC ATCCTGCGTG CTGCCATCCA CAGCGACGCG 
CTCTTCCTGT CCAGCCACCT CATCATTGAT TACTCCCTGC TGGTGGGCCG AGACGATTCC 
ACAGATGAGC TAGTTGTGGG GATCATAGAT TATATCCGGA CTTTCACTTG GGATAAAAAG 
CTGGAGATGG TGGTCAAATC TACTGGGATT CTTGGAGGTC AAGGTAAAAT GCCCACAGTG 
GTGTCACCAG AGCTGTATCG AACACGTTTC TGTGAGGCCA TGGATAAATA CTTCCTTATG 
GTCCCTGATC ACTGGAGTGG ACTTGGGCTC AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302998.1
CDS	164..6502
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQSHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304723.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304723.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAG TATGGAGCCC CATGACCCGT TCACCCCAGA AACCGACATC 
CATGCACCAA TGGAAGTCTT GCTCTCCGAA GATGGAGGTC AGCATATATC CATCAGTGAT 
GCATTCATCA AAGAGTCTTT GTTCAACCGG AGGGTTGAGG AGAAAGCTAA AGAGATGCTC 
TTCACTCCTC TCGGCTGGCA TCACAGCTCT CTGGATCAGC TGCGGGAAGA GAATGGGGAA 
AAGAAAGCGA TGGAGCGGTT GCTGTCAGCA AATCACAGCC ACATGATGGC GCTGCTGCAG 
CAACTGCTGT ACAGTGAATC ACTGTCTCTG TCGTGGCGTG ACATCATCGT ACCTGTGGTG 
AGGCAGGTGG TGCAGACGGT GCGACCGGAT GTGCGCAGCT GTGACGATGA TATGGACATC 
CGTCAGTTTG TCCACATCAA GAAGATTCCA GGAGGAAAGA AGTTTGACTC TGCTGTAGTG 
AATGGTTTTG TTTGCACAAA GAATATCGCA CACAAAAAGA TGAACTCTTA CATCAAAAAC 
CCCAAGATCC TTCTTCTCAA ATGCTCCATT GAGTATCTAT ATAGAGAGGA GACCAAGTTC 
ACCTGCATTG ACCCAATTGT TTTACAGGAG CGTGAGTTTC TTAAGAACTA TGTTCAGAGG 
ATTGTTGATG TCCGACCAAA CTTAGTCCTG GTGGAGAAGA CTGTATCGCG AATTGCTCAG 
GACATGCTAC TAGAGCACGG CATTGCCATT GTGATTAATG TCAAACCTCA AGTACTGGAC 
CGTGTGAGTC GAATGACTCA GGGAGATTTA GTCATTTCAA TGGATCAGCT TCTTACAAAA 
CCTCGTCTGG GTACCTGCCA CAAGTTTTAC TTGCACTCCT TCCAGCTACC AAATAGTGAA 
GTGAAAACCC TGATGTTTTT TGAGGGCTGT CCTCCTCAGC TGGGCTGCAC TATTAAGCTC 
CGCGGAGCCT CAGAGTATGA GCTGGCACGG GTAAAAGAGA TCATCATTTT TATGGTGTGT 
GTGGCATACC ACTCGCAGCT AGAGATCTCT TTCCTCATGG ATGAGTTCGC TATGCCTCCC 
AGTCTGGCTG AGAGTTCTTC TTTCCCATGT CTGCTGGAAA GCACCACTTT ACGGGAAGAG 
GATGAAACTA GTGGGGAACG GGAAAGCCAA GACAATGATG AGTCTACACT GGGAGATGAC 
AATCTCACAC TTGTTCCAGG AGATGACTTT GAGACAGGAT TTACAGGGAC TATCAAAGCC 
CACAGTCGAG AGCCTTCCAC CTCTGAATCT TCTCATAAAG ATGGAGAAAG CCCCAGAATA 
AATAAAAATG CTTCAATTAC CTCCTTCTCT GGAGGGGAGG AAGAAAATAT AAAGACCTCC 
ACGCCACTTT CCTCCTTCTC TTCCACCTTT CCTCAGCCGG TGACACCACC TTTCCTGATA 
TCAGACGTAA AAGAAATGTC ACAGGAAGTG GTCAAGGGGT CAGGTGAAGG GGAGAAGAAC 
AAATATCTGG AGTCGACTCT GGTCCATCAG GACAGCACAA GCTCTGAGAC CTCCCTTCCC 
CCAACTCGGC TCTTTAGAGA CCCCTTACAG GACGACACAG GCCTGTTTGT GACAGAGCAT 
GTAGCTTCTT CAGATGACCG CCTCAAATCC ATCTCTGCTT TATTCAAACA GGAGCTAAAA 
GACATCATCC TGTGCATTTC ACCCTTTATT ACCTTTCGAG AGCCTTTCTT GCTTACCCCA 
GCTGGACTGC GGTGTCCTAG CCGGGATTAT TTCCCTGAAC AGGTTTACCT TTCACCTCTG 
CTGAACAAGG ATTCAAAAGA ACTGGATGGA CGCCGCAAGA GGCAGCTGCT GAAAGAGTCC 
AGCCCAGGTT CTGGTAGCCT GACCAATGGT TCCGTTTCAC ACCAGAGAGC CATTCAGATC 
TTGCCCTGTC ACAAACTCAC TAGTGCCCGA ATAGCAGAAC AGGTAGGCAG CAGTCAGCAG 
CTGGCACGCA TGTTAGCGGA CTACCGTGCC CAAGGAGGAC GTATCCGGCA AAGAGAGGGA 
ACACAATTTC GTGAAGCCCC TCCCCCAAAG GCGCCAATAA AGGCAGATAG TGAAGAAGAT 
AAAGGTGCAG GAGTGATTGA CATGACCTGG TCTACTAAGT TGGACTGTTT AAATCCAATC 
AACCATCAGA GGCTCTGTGT TCTGTTCAGT AGCTCTTCAG CACAGTCTAA TAATGCCCCA 
AACCCCTGCG TCAGTCCCTG GATTGTTACA ATGGAGTTTT ATGGAAAGAA TGACTTGACT 
CTTGGTGTGT TTCTGGAGAG ATACTGTTTT AGACCATCTT ACCAGTGCCC CAGCATGTAC 
TGTGAGACCC CCATGGTTCA CCACATCCGG CGCTTTGTGC ACGGTAAAGG CTGCGTCCAG 
ATTGTACTGA AAGAGCTGGA CTCGCCTGTT CCTGGATATC AGCACACAAT CCTCAACTAC 
TCATGGTGTC GCATCTGCAA ACAGGTGACT CCTGTGGTCC CTTTGTCTAA TGACTCTTGG 
TCCATGTCCT TCGCCAAGTA CCTTGAGCTT CGTTTCTATG GTCATCAGTA CACCCGTCGG 
GCTAACGCTG AGCCTTGTGG CCACTCTATC CATAAAGATT ATCACCAGTA CTTCTCTTAC 
AACCAGATGG TGGCATCCTT CAGCTACATT CCAGTGAGGC TGTTAGAGAT CTGTCTGCCT 
CCCCCGAAGA TCATCATCAG GAACCAAGGT CCATCGAAAT CCAGCTTGCA GCAGGATCTC 
AAAGACTTCT CCCAGAAGGT GGCTCAGGTG TACCTGGCTA TAGATGACCG TCTTACCTCC 
TTAAAAACGG ACACATTCAG CAAGACCAGA GAGGAAAAAA TGGAGGACAT GTTTGCACAG 
AAAGATATGG AGGAATCAGA GCTTCGGGGC TGGATAGAGA AGTTACAAGT GCGTCTGCAG 
ACGAGTGTGA TTGACTCTTC ACAACAACTA CAGGCTGTTC TGGAGTCAGT GGTGGTCAAA 
AAACAGGGCT TGTGTGAAAC CCTGCAGTCC TGGAACAACA GACTTCAAGA ACTGTTCCAG 
CAAGAGAAAG GCAGAAAACG TCTATCTGTT CCTCCTAGCC CAGGCAGACA CAGACAACCT 
ACAACAGATG AGAGCAAGAC TAGTGCTTTG GAGTCTTCTC CTCGTAACCC TTCCCCAGTG 
GTCCCAAATG GAGACAAAGA GGACCGTCAT CTCAATACAT TTCCATCAAG TTCAGGGTCC 
TCGTCGTTAC TACAGTTACC ATCTCCAGCT GAACAACCTT CAGATGTCAT TACAAGCGGA 
CCATCTTTCC CTGATCAGGA CTCTGTTAGC AATCCAGAGG ACATGTTTGA TGGACACTTG 
CTTGGTTCCA ATGATAGTCA GGTAAAGGAA AAGTCCACCA TGAAAACAAT CCTGGCCAAC 
CTGTTACCTG GAAACAGCTA CAATCCCATC CCTTTTCCTT TTGACCCAGA CAAGCACTAT 
CTGATGTATG AGCATGAGAG AGTTCCTATA GCCGTGTGTG AGAGAGAGCC CAGCTCCATA 
ATTGCCTTTG CACTCAGCTG CAAAGAGTAT AAAACTGCCC TTGAAGAGCT CACAAAGACA 
ACAGCAAAGA CTGGAGGTGA TGACACTTCT CAGACCATCA GTGCTGGAGA GAGCAGAGTG 
AAGAACAGTC CAGCCAAACC CAGTGACAAC AGCATGTCAC AGCTGAGCCG CAGCAGCGTC 
GACGCTGATC CACTCAAGGA GCCTGAAAGT GCAGACAAAC AGAAGAAGCA GACAGGGAAC 
CCACATATTG AGCTGCAGTT CTCCGATGCA AATGCTAAGT TCTACTGCCG GATCTACTAT 
GCTGAAGAGT TCCATAAAAT GCGGGAGGAG ATCATGGAGA GCACAGAGGA TGATTTTGTG 
CGATCTCTTT CCCACTGTGT CAACTGGCAG GCGCGTGGTG GGAAATCTGG TGCAGTTTTC 
TATGCCACTG AAGATGATCG GTTTATTCTG AAGCAGATGC CCAGATTAGA GGTCCAGTCC 
TTTTTAGACT TTGCACCCCA CTACTTTACG TACATCACAG GAGCGGTTCA CCTAAAGCGA 
CCCACAGCAC TTGCTAAGAT TCTGGGAGTG TACCGCATCG GCTACAAGAA CTCCCAGAAC 
AACACTGAGA AGAAGCTTGA CCTGCTTGTG ATGGAAAACC TTTTCTATGG ACGAAAGATG 
GCACAGGTGT TTGACCTGAA AGGATCGCTG AGAAACAGGA ACGTGAAGAC GGACCAGGGA 
AAGGAGAGCT GTGAGGTGGT GCTCCTAGAT GAGAACCTGC TCAAACTGGT GCATGACAAT 
CCCCTTTATA TTCGCTCGCA CTGCAAGGCC ATCCTGCGTG CTGCCATCCA CAGCGACGCG 
CTCTTCCTGT CCAGCCACCT CATCATTGAT TACTCCCTGC TGGTGGGCCG AGACGATTCC 
ACAGATGAGC TAGTTGTGGG GATCATAGAT TATATCCGGA CTTTCACTTG GGATAAAAAG 
CTGGAGATGG TGGTCAAATC TACTGGGATT CTTGGAGGTC AAGGTAAAAT GCCCACAGTG 
GTGTCACCAG AGCTGTATCG AACACGTTTC TGTGAGGCCA TGGATAAATA CTTCCTTATG 
GTCCCTGATC ACTGGAGTGG ACTTGGGCTC AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304723.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302998.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.

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CloneID	ODa40124
Clone ID Related Accession (Same CDS sequence)	XM_009304723.3 , XM_009304723.2
Accession Version	XM_009304723.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAG TATGGAGCCC CATGACCCGT TCACCCCAGA AACCGACATC 
CATGCACCAA TGGAAGTCTT GCTCTCCGAA GATGGAGGTC AGCATATATC CATCAGTGAT 
GCATTCATCA AAGAGTCTTT GTTCAACCGG AGGGTTGAGG AGAAAGCTAA AGAGATGCTC 
TTCACTCCTC TCGGCTGGCA TCACAGCTCT CTGGATCAGC TGCGGGAAGA GAATGGGGAA 
AAGAAAGCGA TGGAGCGGTT GCTGTCAGCA AATCACAGCC ACATGATGGC GCTGCTGCAG 
CAACTGCTGT ACAGTGAATC ACTGTCTCTG TCGTGGCGTG ACATCATCGT ACCTGTGGTG 
AGGCAGGTGG TGCAGACGGT GCGACCGGAT GTGCGCAGCT GTGACGATGA TATGGACATC 
CGTCAGTTTG TCCACATCAA GAAGATTCCA GGAGGAAAGA AGTTTGACTC TGCTGTAGTG 
AATGGTTTTG TTTGCACAAA GAATATCGCA CACAAAAAGA TGAACTCTTA CATCAAAAAC 
CCCAAGATCC TTCTTCTCAA ATGCTCCATT GAGTATCTAT ATAGAGAGGA GACCAAGTTC 
ACCTGCATTG ACCCAATTGT TTTACAGGAG CGTGAGTTTC TTAAGAACTA TGTTCAGAGG 
ATTGTTGATG TCCGACCAAA CTTAGTCCTG GTGGAGAAGA CTGTATCGCG AATTGCTCAG 
GACATGCTAC TAGAGCACGG CATTGCCATT GTGATTAATG TCAAACCTCA AGTACTGGAC 
CGTGTGAGTC GAATGACTCA GGGAGATTTA GTCATTTCAA TGGATCAGCT TCTTACAAAA 
CCTCGTCTGG GTACCTGCCA CAAGTTTTAC TTGCACTCCT TCCAGCTACC AAATAGTGAA 
GTGAAAACCC TGATGTTTTT TGAGGGCTGT CCTCCTCAGC TGGGCTGCAC TATTAAGCTC 
CGCGGAGCCT CAGAGTATGA GCTGGCACGG GTAAAAGAGA TCATCATTTT TATGGTGTGT 
GTGGCATACC ACTCGCAGCT AGAGATCTCT TTCCTCATGG ATGAGTTCGC TATGCCTCCC 
AGTCTGGCTG AGAGTTCTTC TTTCCCATGT CTGCTGGAAA GCACCACTTT ACGGGAAGAG 
GATGAAACTA GTGGGGAACG GGAAAGCCAA GACAATGATG AGTCTACACT GGGAGATGAC 
AATCTCACAC TTGTTCCAGG AGATGACTTT GAGACAGGAT TTACAGGGAC TATCAAAGCC 
CACAGTCGAG AGCCTTCCAC CTCTGAATCT TCTCATAAAG ATGGAGAAAG CCCCAGAATA 
AATAAAAATG CTTCAATTAC CTCCTTCTCT GGAGGGGAGG AAGAAAATAT AAAGACCTCC 
ACGCCACTTT CCTCCTTCTC TTCCACCTTT CCTCAGCCGG TGACACCACC TTTCCTGATA 
TCAGACGTAA AAGAAATGTC ACAGGAAGTG GTCAAGGGGT CAGGTGAAGG GGAGAAGAAC 
AAATATCTGG AGTCGACTCT GGTCCATCAG GACAGCACAA GCTCTGAGAC CTCCCTTCCC 
CCAACTCGGC TCTTTAGAGA CCCCTTACAG GACGACACAG GCCTGTTTGT GACAGAGCAT 
GTAGCTTCTT CAGATGACCG CCTCAAATCC ATCTCTGCTT TATTCAAACA GGAGCTAAAA 
GACATCATCC TGTGCATTTC ACCCTTTATT ACCTTTCGAG AGCCTTTCTT GCTTACCCCA 
GCTGGACTGC GGTGTCCTAG CCGGGATTAT TTCCCTGAAC AGGTTTACCT TTCACCTCTG 
CTGAACAAGG ATTCAAAAGA ACTGGATGGA CGCCGCAAGA GGCAGCTGCT GAAAGAGTCC 
AGCCCAGGTT CTGGTAGCCT GACCAATGGT TCCGTTTCAC ACCAGAGAGC CATTCAGATC 
TTGCCCTGTC ACAAACTCAC TAGTGCCCGA ATAGCAGAAC AGGTAGGCAG CAGTCAGCAG 
CTGGCACGCA TGTTAGCGGA CTACCGTGCC CAAGGAGGAC GTATCCGGCA AAGAGAGGGA 
ACACAATTTC GTGAAGCCCC TCCCCCAAAG GCGCCAATAA AGGCAGATAG TGAAGAAGAT 
AAAGGTGCAG GAGTGATTGA CATGACCTGG TCTACTAAGT TGGACTGTTT AAATCCAATC 
AACCATCAGA GGCTCTGTGT TCTGTTCAGT AGCTCTTCAG CACAGTCTAA TAATGCCCCA 
AACCCCTGCG TCAGTCCCTG GATTGTTACA ATGGAGTTTT ATGGAAAGAA TGACTTGACT 
CTTGGTGTGT TTCTGGAGAG ATACTGTTTT AGACCATCTT ACCAGTGCCC CAGCATGTAC 
TGTGAGACCC CCATGGTTCA CCACATCCGG CGCTTTGTGC ACGGTAAAGG CTGCGTCCAG 
ATTGTACTGA AAGAGCTGGA CTCGCCTGTT CCTGGATATC AGCACACAAT CCTCAACTAC 
TCATGGTGTC GCATCTGCAA ACAGGTGACT CCTGTGGTCC CTTTGTCTAA TGACTCTTGG 
TCCATGTCCT TCGCCAAGTA CCTTGAGCTT CGTTTCTATG GTCATCAGTA CACCCGTCGG 
GCTAACGCTG AGCCTTGTGG CCACTCTATC CATAAAGATT ATCACCAGTA CTTCTCTTAC 
AACCAGATGG TGGCATCCTT CAGCTACATT CCAGTGAGGC TGTTAGAGAT CTGTCTGCCT 
CCCCCGAAGA TCATCATCAG GAACCAAGGT CCATCGAAAT CCAGCTTGCA GCAGGATCTC 
AAAGACTTCT CCCAGAAGGT GGCTCAGGTG TACCTGGCTA TAGATGACCG TCTTACCTCC 
TTAAAAACGG ACACATTCAG CAAGACCAGA GAGGAAAAAA TGGAGGACAT GTTTGCACAG 
AAAGATATGG AGGAATCAGA GCTTCGGGGC TGGATAGAGA AGTTACAAGT GCGTCTGCAG 
ACGAGTGTGA TTGACTCTTC ACAACAACTA CAGGCTGTTC TGGAGTCAGT GGTGGTCAAA 
AAACAGGGCT TGTGTGAAAC CCTGCAGTCC TGGAACAACA GACTTCAAGA ACTGTTCCAG 
CAAGAGAAAG GCAGAAAACG TCTATCTGTT CCTCCTAGCC CAGGCAGACA CAGACAACCT 
ACAACAGATG AGAGCAAGAC TAGTGCTTTG GAGTCTTCTC CTCGTAACCC TTCCCCAGTG 
GTCCCAAATG GAGACAAAGA GGACCGTCAT CTCAATACAT TTCCATCAAG TTCAGGGTCC 
TCGTCGTTAC TACAGTTACC ATCTCCAGCT GAACAACCTT CAGATGTCAT TACAAGCGGA 
CCATCTTTCC CTGATCAGGA CTCTGTTAGC AATCCAGAGG ACATGTTTGA TGGACACTTG 
CTTGGTTCCA ATGATAGTCA GGTAAAGGAA AAGTCCACCA TGAAAACAAT CCTGGCCAAC 
CTGTTACCTG GAAACAGCTA CAATCCCATC CCTTTTCCTT TTGACCCAGA CAAGCACTAT 
CTGATGTATG AGCATGAGAG AGTTCCTATA GCCGTGTGTG AGAGAGAGCC CAGCTCCATA 
ATTGCCTTTG CACTCAGCTG CAAAGAGTAT AAAACTGCCC TTGAAGAGCT CACAAAGACA 
ACAGCAAAGA CTGGAGGTGA TGACACTTCT CAGACCATCA GTGCTGGAGA GAGCAGAGTG 
AAGAACAGTC CAGCCAAACC CAGTGACAAC AGCATGTCAC AGCTGAGCCG CAGCAGCGTC 
GACGCTGATC CACTCAAGGA GCCTGAAAGT GCAGACAAAC AGAAGAAGCA GACAGGGAAC 
CCACATATTG AGCTGCAGTT CTCCGATGCA AATGCTAAGT TCTACTGCCG GATCTACTAT 
GCTGAAGAGT TCCATAAAAT GCGGGAGGAG ATCATGGAGA GCACAGAGGA TGATTTTGTG 
CGATCTCTTT CCCACTGTGT CAACTGGCAG GCGCGTGGTG GGAAATCTGG TGCAGTTTTC 
TATGCCACTG AAGATGATCG GTTTATTCTG AAGCAGATGC CCAGATTAGA GGTCCAGTCC 
TTTTTAGACT TTGCACCCCA CTACTTTACG TACATCACAG GAGCGGTTCA CCTAAAGCGA 
CCCACAGCAC TTGCTAAGAT TCTGGGAGTG TACCGCATCG GCTACAAGAA CTCCCAGAAC 
AACACTGAGA AGAAGCTTGA CCTGCTTGTG ATGGAAAACC TTTTCTATGG ACGAAAGATG 
GCACAGGTGT TTGACCTGAA AGGATCGCTG AGAAACAGGA ACGTGAAGAC GGACCAGGGA 
AAGGAGAGCT GTGAGGTGGT GCTCCTAGAT GAGAACCTGC TCAAACTGGT GCATGACAAT 
CCCCTTTATA TTCGCTCGCA CTGCAAGGCC ATCCTGCGTG CTGCCATCCA CAGCGACGCG 
CTCTTCCTGT CCAGCCACCT CATCATTGAT TACTCCCTGC TGGTGGGCCG AGACGATTCC 
ACAGATGAGC TAGTTGTGGG GATCATAGAT TATATCCGGA CTTTCACTTG GGATAAAAAG 
CTGGAGATGG TGGTCAAATC TACTGGGATT CTTGGAGGTC AAGGTAAAAT GCCCACAGTG 
GTGTCACCAG AGCTGTATCG AACACGTTTC TGTGAGGCCA TGGATAAATA CTTCCTTATG 
GTCCCTGATC ACTGGAGTGG ACTTGGGCTC AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302998.1
CDS	156..6494
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQSHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304723.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304723.2

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAG TATGGAGCCC CATGACCCGT TCACCCCAGA AACCGACATC 
CATGCACCAA TGGAAGTCTT GCTCTCCGAA GATGGAGGTC AGCATATATC CATCAGTGAT 
GCATTCATCA AAGAGTCTTT GTTCAACCGG AGGGTTGAGG AGAAAGCTAA AGAGATGCTC 
TTCACTCCTC TCGGCTGGCA TCACAGCTCT CTGGATCAGC TGCGGGAAGA GAATGGGGAA 
AAGAAAGCGA TGGAGCGGTT GCTGTCAGCA AATCACAGCC ACATGATGGC GCTGCTGCAG 
CAACTGCTGT ACAGTGAATC ACTGTCTCTG TCGTGGCGTG ACATCATCGT ACCTGTGGTG 
AGGCAGGTGG TGCAGACGGT GCGACCGGAT GTGCGCAGCT GTGACGATGA TATGGACATC 
CGTCAGTTTG TCCACATCAA GAAGATTCCA GGAGGAAAGA AGTTTGACTC TGCTGTAGTG 
AATGGTTTTG TTTGCACAAA GAATATCGCA CACAAAAAGA TGAACTCTTA CATCAAAAAC 
CCCAAGATCC TTCTTCTCAA ATGCTCCATT GAGTATCTAT ATAGAGAGGA GACCAAGTTC 
ACCTGCATTG ACCCAATTGT TTTACAGGAG CGTGAGTTTC TTAAGAACTA TGTTCAGAGG 
ATTGTTGATG TCCGACCAAA CTTAGTCCTG GTGGAGAAGA CTGTATCGCG AATTGCTCAG 
GACATGCTAC TAGAGCACGG CATTGCCATT GTGATTAATG TCAAACCTCA AGTACTGGAC 
CGTGTGAGTC GAATGACTCA GGGAGATTTA GTCATTTCAA TGGATCAGCT TCTTACAAAA 
CCTCGTCTGG GTACCTGCCA CAAGTTTTAC TTGCACTCCT TCCAGCTACC AAATAGTGAA 
GTGAAAACCC TGATGTTTTT TGAGGGCTGT CCTCCTCAGC TGGGCTGCAC TATTAAGCTC 
CGCGGAGCCT CAGAGTATGA GCTGGCACGG GTAAAAGAGA TCATCATTTT TATGGTGTGT 
GTGGCATACC ACTCGCAGCT AGAGATCTCT TTCCTCATGG ATGAGTTCGC TATGCCTCCC 
AGTCTGGCTG AGAGTTCTTC TTTCCCATGT CTGCTGGAAA GCACCACTTT ACGGGAAGAG 
GATGAAACTA GTGGGGAACG GGAAAGCCAA GACAATGATG AGTCTACACT GGGAGATGAC 
AATCTCACAC TTGTTCCAGG AGATGACTTT GAGACAGGAT TTACAGGGAC TATCAAAGCC 
CACAGTCGAG AGCCTTCCAC CTCTGAATCT TCTCATAAAG ATGGAGAAAG CCCCAGAATA 
AATAAAAATG CTTCAATTAC CTCCTTCTCT GGAGGGGAGG AAGAAAATAT AAAGACCTCC 
ACGCCACTTT CCTCCTTCTC TTCCACCTTT CCTCAGCCGG TGACACCACC TTTCCTGATA 
TCAGACGTAA AAGAAATGTC ACAGGAAGTG GTCAAGGGGT CAGGTGAAGG GGAGAAGAAC 
AAATATCTGG AGTCGACTCT GGTCCATCAG GACAGCACAA GCTCTGAGAC CTCCCTTCCC 
CCAACTCGGC TCTTTAGAGA CCCCTTACAG GACGACACAG GCCTGTTTGT GACAGAGCAT 
GTAGCTTCTT CAGATGACCG CCTCAAATCC ATCTCTGCTT TATTCAAACA GGAGCTAAAA 
GACATCATCC TGTGCATTTC ACCCTTTATT ACCTTTCGAG AGCCTTTCTT GCTTACCCCA 
GCTGGACTGC GGTGTCCTAG CCGGGATTAT TTCCCTGAAC AGGTTTACCT TTCACCTCTG 
CTGAACAAGG ATTCAAAAGA ACTGGATGGA CGCCGCAAGA GGCAGCTGCT GAAAGAGTCC 
AGCCCAGGTT CTGGTAGCCT GACCAATGGT TCCGTTTCAC ACCAGAGAGC CATTCAGATC 
TTGCCCTGTC ACAAACTCAC TAGTGCCCGA ATAGCAGAAC AGGTAGGCAG CAGTCAGCAG 
CTGGCACGCA TGTTAGCGGA CTACCGTGCC CAAGGAGGAC GTATCCGGCA AAGAGAGGGA 
ACACAATTTC GTGAAGCCCC TCCCCCAAAG GCGCCAATAA AGGCAGATAG TGAAGAAGAT 
AAAGGTGCAG GAGTGATTGA CATGACCTGG TCTACTAAGT TGGACTGTTT AAATCCAATC 
AACCATCAGA GGCTCTGTGT TCTGTTCAGT AGCTCTTCAG CACAGTCTAA TAATGCCCCA 
AACCCCTGCG TCAGTCCCTG GATTGTTACA ATGGAGTTTT ATGGAAAGAA TGACTTGACT 
CTTGGTGTGT TTCTGGAGAG ATACTGTTTT AGACCATCTT ACCAGTGCCC CAGCATGTAC 
TGTGAGACCC CCATGGTTCA CCACATCCGG CGCTTTGTGC ACGGTAAAGG CTGCGTCCAG 
ATTGTACTGA AAGAGCTGGA CTCGCCTGTT CCTGGATATC AGCACACAAT CCTCAACTAC 
TCATGGTGTC GCATCTGCAA ACAGGTGACT CCTGTGGTCC CTTTGTCTAA TGACTCTTGG 
TCCATGTCCT TCGCCAAGTA CCTTGAGCTT CGTTTCTATG GTCATCAGTA CACCCGTCGG 
GCTAACGCTG AGCCTTGTGG CCACTCTATC CATAAAGATT ATCACCAGTA CTTCTCTTAC 
AACCAGATGG TGGCATCCTT CAGCTACATT CCAGTGAGGC TGTTAGAGAT CTGTCTGCCT 
CCCCCGAAGA TCATCATCAG GAACCAAGGT CCATCGAAAT CCAGCTTGCA GCAGGATCTC 
AAAGACTTCT CCCAGAAGGT GGCTCAGGTG TACCTGGCTA TAGATGACCG TCTTACCTCC 
TTAAAAACGG ACACATTCAG CAAGACCAGA GAGGAAAAAA TGGAGGACAT GTTTGCACAG 
AAAGATATGG AGGAATCAGA GCTTCGGGGC TGGATAGAGA AGTTACAAGT GCGTCTGCAG 
ACGAGTGTGA TTGACTCTTC ACAACAACTA CAGGCTGTTC TGGAGTCAGT GGTGGTCAAA 
AAACAGGGCT TGTGTGAAAC CCTGCAGTCC TGGAACAACA GACTTCAAGA ACTGTTCCAG 
CAAGAGAAAG GCAGAAAACG TCTATCTGTT CCTCCTAGCC CAGGCAGACA CAGACAACCT 
ACAACAGATG AGAGCAAGAC TAGTGCTTTG GAGTCTTCTC CTCGTAACCC TTCCCCAGTG 
GTCCCAAATG GAGACAAAGA GGACCGTCAT CTCAATACAT TTCCATCAAG TTCAGGGTCC 
TCGTCGTTAC TACAGTTACC ATCTCCAGCT GAACAACCTT CAGATGTCAT TACAAGCGGA 
CCATCTTTCC CTGATCAGGA CTCTGTTAGC AATCCAGAGG ACATGTTTGA TGGACACTTG 
CTTGGTTCCA ATGATAGTCA GGTAAAGGAA AAGTCCACCA TGAAAACAAT CCTGGCCAAC 
CTGTTACCTG GAAACAGCTA CAATCCCATC CCTTTTCCTT TTGACCCAGA CAAGCACTAT 
CTGATGTATG AGCATGAGAG AGTTCCTATA GCCGTGTGTG AGAGAGAGCC CAGCTCCATA 
ATTGCCTTTG CACTCAGCTG CAAAGAGTAT AAAACTGCCC TTGAAGAGCT CACAAAGACA 
ACAGCAAAGA CTGGAGGTGA TGACACTTCT CAGACCATCA GTGCTGGAGA GAGCAGAGTG 
AAGAACAGTC CAGCCAAACC CAGTGACAAC AGCATGTCAC AGCTGAGCCG CAGCAGCGTC 
GACGCTGATC CACTCAAGGA GCCTGAAAGT GCAGACAAAC AGAAGAAGCA GACAGGGAAC 
CCACATATTG AGCTGCAGTT CTCCGATGCA AATGCTAAGT TCTACTGCCG GATCTACTAT 
GCTGAAGAGT TCCATAAAAT GCGGGAGGAG ATCATGGAGA GCACAGAGGA TGATTTTGTG 
CGATCTCTTT CCCACTGTGT CAACTGGCAG GCGCGTGGTG GGAAATCTGG TGCAGTTTTC 
TATGCCACTG AAGATGATCG GTTTATTCTG AAGCAGATGC CCAGATTAGA GGTCCAGTCC 
TTTTTAGACT TTGCACCCCA CTACTTTACG TACATCACAG GAGCGGTTCA CCTAAAGCGA 
CCCACAGCAC TTGCTAAGAT TCTGGGAGTG TACCGCATCG GCTACAAGAA CTCCCAGAAC 
AACACTGAGA AGAAGCTTGA CCTGCTTGTG ATGGAAAACC TTTTCTATGG ACGAAAGATG 
GCACAGGTGT TTGACCTGAA AGGATCGCTG AGAAACAGGA ACGTGAAGAC GGACCAGGGA 
AAGGAGAGCT GTGAGGTGGT GCTCCTAGAT GAGAACCTGC TCAAACTGGT GCATGACAAT 
CCCCTTTATA TTCGCTCGCA CTGCAAGGCC ATCCTGCGTG CTGCCATCCA CAGCGACGCG 
CTCTTCCTGT CCAGCCACCT CATCATTGAT TACTCCCTGC TGGTGGGCCG AGACGATTCC 
ACAGATGAGC TAGTTGTGGG GATCATAGAT TATATCCGGA CTTTCACTTG GGATAAAAAG 
CTGGAGATGG TGGTCAAATC TACTGGGATT CTTGGAGGTC AAGGTAAAAT GCCCACAGTG 
GTGTCACCAG AGCTGTATCG AACACGTTTC TGTGAGGCCA TGGATAAATA CTTCCTTATG 
GTCCCTGATC ACTGGAGTGG ACTTGGGCTC AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304724.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302999.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.

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CloneID	ODa40125
Clone ID Related Accession (Same CDS sequence)	XM_009304724.3 , XM_009304724.2
Accession Version	XM_009304724.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304724.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG CGGAGTGGGA AGAGGGTAGA 
CGGAAATCAG AAGCTCCTCT GGGCAGCCAC GATCCCAGAA CAGCTGTCCA GCTTCGCACA 
GCTCTCAAGA GGCTCAAAGA GATCATGGAA GGGAAAAGTC AGGACAGTGA TCTGAAACAG 
TACTGGATGC CAGATAGCCA GTGTAAGGAG TGCTATGACT GCAATGAGAA ATTCACAACC 
TTCCGACGCC GTCACCATTG TCGACTATGT GGACAAATAT TCTGCAGCCG ATGCTGCAAC 
CAGGAAATTC CTGGCAAGTT CATGGGCTAC ACGGGCGATT TAAGGGCTTG TACATACTGT 
CGTAAGATAG CATTAAGCTA TGCTCACTCA ACAGACTCAA GCTCCATTGG AGAAGATCTA 
AGTGCTTTGT CGGACTCGCC TTGCTCCGTC TGTGTTCTGG AGCCCACTGA ACCACGCACA 
CCTGTAGGAG GTCGCAAAGC CAGCCGCAAT ATCTTCCTGG AAGAGGACCT AGCCTGGCAA 
AGAAAAAATT CCATTGGGAT GAGGAAGAAT CATCAGGAAT CTCAGAACAG CGGTCTCAAT 
TCCAGACTGA CAGTGGTCCA AGAGGATATT GGAAAGTCAC CTGCCAGGAA GAGGTCAGCT 
AGTGTGACCA ATTTGTCTTT GGACCGTTCC GGCTCATCCA TGGTTCCTGC CTATGAGAGT 
TCGGTTAGTC CACAGAACAG CCGAGCTCTA CCCAAGACCG ACCACAGCGA AGAAGAGCGA 
AAGATCCTTC TAGATTCTTT TCAACTTAAA GATATATGGA AGAAGATTTG CCACAACACT 
ACTGGGATGG AGTTCCAGGA CCATCGGTAC TGGCTGCGCA CTTACCCCAA CTGCATTGTG 
GGTAAAGAGT TGGTCAACTG GCTTTTAAGA AATGGCACTA TTTCCACTAG GGCTCAAGCC 
ATAGCTATAG GACAGGCTTT GGTAGATGGC CGCTGGCTTG ACTGTGTAAC CCATCATGAT 
CAGATCTTCC GTGATGAGTA CGCCCTTTAT CGCCCCCTTC AGAGCACAGA GTTCTCCGAA 
ACTCCTTCTC CAGACAGTGA CAGTGTTAAT TCCCTGGAGG GACACTCTGA ACCGTCATGG 
TTTAAAGACA TCAAGTTTGA TGACAGTGAC ACCGAGCAGC TAGCAGATGA AAATGACTAT 
GTCACACCAA GCTCGTCCAG CCCCAGCAAA AGAACATCTG TCAGCAGTTT CCACTCTGCA 
GTAGACAGTG ACTCGGCCGC CTCCATCAGT CTAAACATGG AGCAGGACAA CGTCAATTTC 
CACATTAAGA AGCAGGCCAA GTACCCCCAT GTGCCTCCCT ACCCAGCTGA GCAAAAAAGT 
ATGGAGCCCC ATGACCCGTT CACCCCAGAA ACCGACATCC ATGCACCAAT GGAAGTCTTG 
CTCTCCGAAG ATGGAGGTCA GCATATATCC ATCAGTGATG CATTCATCAA AGAGTCTTTG 
TTCAACCGGA GGGTTGAGGA GAAAGCTAAA GAGATGCTCT TCACTCCTCT CGGCTGGCAT 
CACAGCTCTC TGGATCAGCT GCGGGAAGAG AATGGGGAAA AGAAAGCGAT GGAGCGGTTG 
CTGTCAGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AACTGCTGTA CAGTGAATCA 
CTGTCTCTGT CGTGGCGTGA CATCATCGTA CCTGTGGTGA GGCAGGTGGT GCAGACGGTG 
CGACCGGATG TGCGCAGCTG TGACGATGAT ATGGACATCC GTCAGTTTGT CCACATCAAG 
AAGATTCCAG GAGGAAAGAA GTTTGACTCT GCTGTAGTGA ATGGTTTTGT TTGCACAAAG 
AATATCGCAC ACAAAAAGAT GAACTCTTAC ATCAAAAACC CCAAGATCCT TCTTCTCAAA 
TGCTCCATTG AGTATCTATA TAGAGAGGAG ACCAAGTTCA CCTGCATTGA CCCAATTGTT 
TTACAGGAGC GTGAGTTTCT TAAGAACTAT GTTCAGAGGA TTGTTGATGT CCGACCAAAC 
TTAGTCCTGG TGGAGAAGAC TGTATCGCGA ATTGCTCAGG ACATGCTACT AGAGCACGGC 
ATTGCCATTG TGATTAATGT CAAACCTCAA GTACTGGACC GTGTGAGTCG AATGACTCAG 
GGAGATTTAG TCATTTCAAT GGATCAGCTT CTTACAAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACT TGCACTCCTT CCAGCTACCA AATAGTGAAG TGAAAACCCT GATGTTTTTT 
GAGGGCTGTC CTCCTCAGCT GGGCTGCACT ATTAAGCTCC GCGGAGCCTC AGAGTATGAG 
CTGGCACGGG TAAAAGAGAT CATCATTTTT ATGGTGTGTG TGGCATACCA CTCGCAGCTA 
GAGATCTCTT TCCTCATGGA TGAGTTCGCT ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT 
TTCCCATGTC TGCTGGAAAG CACCACTTTA CGGGAAGAGG ATGAAACTAG TGGGGAACGG 
GAAAGCCAAG ACAATGATGA GTCTACACTG GGAGATGACA ATCTCACACT TGTTCCAGGA 
GATGACTTTG AGACAGGATT TACAGGGACT ATCAAAGCCC ACAGTCGAGA GCCTTCCACC 
TCTGAATCTT CTCATAAAGA TGGAGAAAGC CCCAGAATAA ATAAAAATGC TTCAATTACC 
TCCTTCTCTG GAGGGGAGGA AGAAAATATA AAGACCTCCA CGCCACTTTC CTCCTTCTCT 
TCCACCTTTC CTCAGCCGGT GACACCACCT TTCCTGATAT CAGACGTAAA AGAAATGTCA 
CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG GAGAAGAACA AATATCTGGA GTCGACTCTG 
GTCCATCAGG ACAGCACAAG CTCTGAGACC TCCCTTCCCC CAACTCGGCT CTTTAGAGAC 
CCCTTACAGG ACGACACAGG CCTGTTTGTG ACAGAGCATG TAGCTTCTTC AGATGACCGC 
CTCAAATCCA TCTCTGCTTT ATTCAAACAG GAGCTAAAAG ACATCATCCT GTGCATTTCA 
CCCTTTATTA CCTTTCGAGA GCCTTTCTTG CTTACCCCAG CTGGACTGCG GTGTCCTAGC 
CGGGATTATT TCCCTGAACA GGTTTACCTT TCACCTCTGC TGAACAAGGA TTCAAAAGAA 
CTGGATGGAC GCCGCAAGAG GCAGCTGCTG AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG 
ACCAATGGTT CCGTTTCACA CCAGAGAGCC ATTCAGATCT TGCCCTGTCA CAAACTCACT 
AGTGCCCGAA TAGCAGAACA GGTAGGCAGC AGTCAGCAGC TGGCACGCAT GTTAGCGGAC 
TACCGTGCCC AAGGAGGACG TATCCGGCAA AGAGAGGGAA CACAATTTCG TGAAGCCCCT 
CCCCCAAAGG CGCCAATAAA GGCAGATAGT GAAGAAGATA AAGGTGCAGG AGTGATTGAC 
ATGACCTGGT CTACTAAGTT GGACTGTTTA AATCCAATCA ACCATCAGAG GCTCTGTGTT 
CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT AATGCCCCAA ACCCCTGCGT CAGTCCCTGG 
ATTGTTACAA TGGAGTTTTA TGGAAAGAAT GACTTGACTC TTGGTGTGTT TCTGGAGAGA 
TACTGTTTTA GACCATCTTA CCAGTGCCCC AGCATGTACT GTGAGACCCC CATGGTTCAC 
CACATCCGGC GCTTTGTGCA CGGTAAAGGC TGCGTCCAGA TTGTACTGAA AGAGCTGGAC 
TCGCCTGTTC CTGGATATCA GCACACAATC CTCAACTACT CATGGTGTCG CATCTGCAAA 
CAGGTGACTC CTGTGGTCCC TTTGTCTAAT GACTCTTGGT CCATGTCCTT CGCCAAGTAC 
CTTGAGCTTC GTTTCTATGG TCATCAGTAC ACCCGTCGGG CTAACGCTGA GCCTTGTGGC 
CACTCTATCC ATAAAGATTA TCACCAGTAC TTCTCTTACA ACCAGATGGT GGCATCCTTC 
AGCTACATTC CAGTGAGGCT GTTAGAGATC TGTCTGCCTC CCCCGAAGAT CATCATCAGG 
AACCAAGGTC CATCGAAATC CAGCTTGCAG CAGGATCTCA AAGACTTCTC CCAGAAGGTG 
GCTCAGGTGT ACCTGGCTAT AGATGACCGT CTTACCTCCT TAAAAACGGA CACATTCAGC 
AAGACCAGAG AGGAAAAAAT GGAGGACATG TTTGCACAGA AAGATATGGA GGAATCAGAG 
CTTCGGGGCT GGATAGAGAA GTTACAAGTG CGTCTGCAGA CGAGTGTGAT TGACTCTTCA 
CAACAACTAC AGGCTGTTCT GGAGTCAGTG GTGGTCAAAA AACAGGGCTT GTGTGAAACC 
CTGCAGTCCT GGAACAACAG ACTTCAAGAA CTGTTCCAGC AAGAGAAAGG CAGAAAACGT 
CTATCTGTTC CTCCTAGCCC AGGCAGACAC AGACAACCTA CAACAGATGA GAGCAAGACT 
AGTGCTTTGG AGTCTTCTCC TCGTAACCCT TCCCCAGTGG TCCCAAATGG AGACAAAGAG 
GACCGTCATC TCAATACATT TCCATCAAGT TCAGGGTCCT CGTCGTTACT ACAGTTACCA 
TCTCCAGCTG AACAACCTTC AGATGTCATT ACAAGCGGAC CATCTTTCCC TGATCAGGAC 
TCTGTTAGCA ATCCAGAGGA CATGTTTGAT GGACACTTGC TTGGTTCCAA TGATAGTCAG 
GTAAAGGAAA AGTCCACCAT GAAAACAATC CTGGCCAACC TGTTACCTGG AAACAGCTAC 
AATCCCATCC CTTTTCCTTT TGACCCAGAC AAGCACTATC TGATGTATGA GCATGAGAGA 
GTTCCTATAG CCGTGTGTGA GAGAGAGCCC AGCTCCATAA TTGCCTTTGC ACTCAGCTGC 
AAAGAGTATA AAACTGCCCT TGAAGAGCTC ACAAAGACAA CAGCAAAGAC TGGAGGTGAT 
GACACTTCTC AGACCATCAG TGCTGGAGAG AGCAGAGTGA AGAACAGTCC AGCCAAACCC 
AGTGACAACA GCATGTCACA GCTGAGCCGC AGCAGCGTCG ACGCTGATCC ACTCAAGGAG 
CCTGAAAGTG CAGACAAACA GAAGAAGCAG ACAGGGAACC CACATATTGA GCTGCAGTTC 
TCCGATGCAA ATGCTAAGTT CTACTGCCGG ATCTACTATG CTGAAGAGTT CCATAAAATG 
CGGGAGGAGA TCATGGAGAG CACAGAGGAT GATTTTGTGC GATCTCTTTC CCACTGTGTC 
AACTGGCAGG CGCGTGGTGG GAAATCTGGT GCAGTTTTCT ATGCCACTGA AGATGATCGG 
TTTATTCTGA AGCAGATGCC CAGATTAGAG GTCCAGTCCT TTTTAGACTT TGCACCCCAC 
TACTTTACGT ACATCACAGG AGCGGTTCAC CTAAAGCGAC CCACAGCACT TGCTAAGATT 
CTGGGAGTGT ACCGCATCGG CTACAAGAAC TCCCAGAACA ACACTGAGAA GAAGCTTGAC 
CTGCTTGTGA TGGAAAACCT TTTCTATGGA CGAAAGATGG CACAGGTGTT TGACCTGAAA 
GGATCGCTGA GAAACAGGAA CGTGAAGACG GACCAGGGAA AGGAGAGCTG TGAGGTGGTG 
CTCCTAGATG AGAACCTGCT CAAACTGGTG CATGACAATC CCCTTTATAT TCGCTCGCAC 
TGCAAGGCCA TCCTGCGTGC TGCCATCCAC AGCGACGCGC TCTTCCTGTC CAGCCACCTC 
ATCATTGATT ACTCCCTGCT GGTGGGCCGA GACGATTCCA CAGATGAGCT AGTTGTGGGG 
ATCATAGATT ATATCCGGAC TTTCACTTGG GATAAAAAGC TGGAGATGGT GGTCAAATCT 
ACTGGGATTC TTGGAGGTCA AGGTAAAATG CCCACAGTGG TGTCACCAGA GCTGTATCGA 
ACACGTTTCT GTGAGGCCAT GGATAAATAC TTCCTTATGG TCCCTGATCA CTGGAGTGGA 
CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302999.1
CDS	164..6481
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304724.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304724.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG CGGAGTGGGA AGAGGGTAGA 
CGGAAATCAG AAGCTCCTCT GGGCAGCCAC GATCCCAGAA CAGCTGTCCA GCTTCGCACA 
GCTCTCAAGA GGCTCAAAGA GATCATGGAA GGGAAAAGTC AGGACAGTGA TCTGAAACAG 
TACTGGATGC CAGATAGCCA GTGTAAGGAG TGCTATGACT GCAATGAGAA ATTCACAACC 
TTCCGACGCC GTCACCATTG TCGACTATGT GGACAAATAT TCTGCAGCCG ATGCTGCAAC 
CAGGAAATTC CTGGCAAGTT CATGGGCTAC ACGGGCGATT TAAGGGCTTG TACATACTGT 
CGTAAGATAG CATTAAGCTA TGCTCACTCA ACAGACTCAA GCTCCATTGG AGAAGATCTA 
AGTGCTTTGT CGGACTCGCC TTGCTCCGTC TGTGTTCTGG AGCCCACTGA ACCACGCACA 
CCTGTAGGAG GTCGCAAAGC CAGCCGCAAT ATCTTCCTGG AAGAGGACCT AGCCTGGCAA 
AGAAAAAATT CCATTGGGAT GAGGAAGAAT CATCAGGAAT CTCAGAACAG CGGTCTCAAT 
TCCAGACTGA CAGTGGTCCA AGAGGATATT GGAAAGTCAC CTGCCAGGAA GAGGTCAGCT 
AGTGTGACCA ATTTGTCTTT GGACCGTTCC GGCTCATCCA TGGTTCCTGC CTATGAGAGT 
TCGGTTAGTC CACAGAACAG CCGAGCTCTA CCCAAGACCG ACCACAGCGA AGAAGAGCGA 
AAGATCCTTC TAGATTCTTT TCAACTTAAA GATATATGGA AGAAGATTTG CCACAACACT 
ACTGGGATGG AGTTCCAGGA CCATCGGTAC TGGCTGCGCA CTTACCCCAA CTGCATTGTG 
GGTAAAGAGT TGGTCAACTG GCTTTTAAGA AATGGCACTA TTTCCACTAG GGCTCAAGCC 
ATAGCTATAG GACAGGCTTT GGTAGATGGC CGCTGGCTTG ACTGTGTAAC CCATCATGAT 
CAGATCTTCC GTGATGAGTA CGCCCTTTAT CGCCCCCTTC AGAGCACAGA GTTCTCCGAA 
ACTCCTTCTC CAGACAGTGA CAGTGTTAAT TCCCTGGAGG GACACTCTGA ACCGTCATGG 
TTTAAAGACA TCAAGTTTGA TGACAGTGAC ACCGAGCAGC TAGCAGATGA AAATGACTAT 
GTCACACCAA GCTCGTCCAG CCCCAGCAAA AGAACATCTG TCAGCAGTTT CCACTCTGCA 
GTAGACAGTG ACTCGGCCGC CTCCATCAGT CTAAACATGG AGCAGGACAA CGTCAATTTC 
CACATTAAGA AGCAGGCCAA GTACCCCCAT GTGCCTCCCT ACCCAGCTGA GCAAAAAAGT 
ATGGAGCCCC ATGACCCGTT CACCCCAGAA ACCGACATCC ATGCACCAAT GGAAGTCTTG 
CTCTCCGAAG ATGGAGGTCA GCATATATCC ATCAGTGATG CATTCATCAA AGAGTCTTTG 
TTCAACCGGA GGGTTGAGGA GAAAGCTAAA GAGATGCTCT TCACTCCTCT CGGCTGGCAT 
CACAGCTCTC TGGATCAGCT GCGGGAAGAG AATGGGGAAA AGAAAGCGAT GGAGCGGTTG 
CTGTCAGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AACTGCTGTA CAGTGAATCA 
CTGTCTCTGT CGTGGCGTGA CATCATCGTA CCTGTGGTGA GGCAGGTGGT GCAGACGGTG 
CGACCGGATG TGCGCAGCTG TGACGATGAT ATGGACATCC GTCAGTTTGT CCACATCAAG 
AAGATTCCAG GAGGAAAGAA GTTTGACTCT GCTGTAGTGA ATGGTTTTGT TTGCACAAAG 
AATATCGCAC ACAAAAAGAT GAACTCTTAC ATCAAAAACC CCAAGATCCT TCTTCTCAAA 
TGCTCCATTG AGTATCTATA TAGAGAGGAG ACCAAGTTCA CCTGCATTGA CCCAATTGTT 
TTACAGGAGC GTGAGTTTCT TAAGAACTAT GTTCAGAGGA TTGTTGATGT CCGACCAAAC 
TTAGTCCTGG TGGAGAAGAC TGTATCGCGA ATTGCTCAGG ACATGCTACT AGAGCACGGC 
ATTGCCATTG TGATTAATGT CAAACCTCAA GTACTGGACC GTGTGAGTCG AATGACTCAG 
GGAGATTTAG TCATTTCAAT GGATCAGCTT CTTACAAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACT TGCACTCCTT CCAGCTACCA AATAGTGAAG TGAAAACCCT GATGTTTTTT 
GAGGGCTGTC CTCCTCAGCT GGGCTGCACT ATTAAGCTCC GCGGAGCCTC AGAGTATGAG 
CTGGCACGGG TAAAAGAGAT CATCATTTTT ATGGTGTGTG TGGCATACCA CTCGCAGCTA 
GAGATCTCTT TCCTCATGGA TGAGTTCGCT ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT 
TTCCCATGTC TGCTGGAAAG CACCACTTTA CGGGAAGAGG ATGAAACTAG TGGGGAACGG 
GAAAGCCAAG ACAATGATGA GTCTACACTG GGAGATGACA ATCTCACACT TGTTCCAGGA 
GATGACTTTG AGACAGGATT TACAGGGACT ATCAAAGCCC ACAGTCGAGA GCCTTCCACC 
TCTGAATCTT CTCATAAAGA TGGAGAAAGC CCCAGAATAA ATAAAAATGC TTCAATTACC 
TCCTTCTCTG GAGGGGAGGA AGAAAATATA AAGACCTCCA CGCCACTTTC CTCCTTCTCT 
TCCACCTTTC CTCAGCCGGT GACACCACCT TTCCTGATAT CAGACGTAAA AGAAATGTCA 
CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG GAGAAGAACA AATATCTGGA GTCGACTCTG 
GTCCATCAGG ACAGCACAAG CTCTGAGACC TCCCTTCCCC CAACTCGGCT CTTTAGAGAC 
CCCTTACAGG ACGACACAGG CCTGTTTGTG ACAGAGCATG TAGCTTCTTC AGATGACCGC 
CTCAAATCCA TCTCTGCTTT ATTCAAACAG GAGCTAAAAG ACATCATCCT GTGCATTTCA 
CCCTTTATTA CCTTTCGAGA GCCTTTCTTG CTTACCCCAG CTGGACTGCG GTGTCCTAGC 
CGGGATTATT TCCCTGAACA GGTTTACCTT TCACCTCTGC TGAACAAGGA TTCAAAAGAA 
CTGGATGGAC GCCGCAAGAG GCAGCTGCTG AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG 
ACCAATGGTT CCGTTTCACA CCAGAGAGCC ATTCAGATCT TGCCCTGTCA CAAACTCACT 
AGTGCCCGAA TAGCAGAACA GGTAGGCAGC AGTCAGCAGC TGGCACGCAT GTTAGCGGAC 
TACCGTGCCC AAGGAGGACG TATCCGGCAA AGAGAGGGAA CACAATTTCG TGAAGCCCCT 
CCCCCAAAGG CGCCAATAAA GGCAGATAGT GAAGAAGATA AAGGTGCAGG AGTGATTGAC 
ATGACCTGGT CTACTAAGTT GGACTGTTTA AATCCAATCA ACCATCAGAG GCTCTGTGTT 
CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT AATGCCCCAA ACCCCTGCGT CAGTCCCTGG 
ATTGTTACAA TGGAGTTTTA TGGAAAGAAT GACTTGACTC TTGGTGTGTT TCTGGAGAGA 
TACTGTTTTA GACCATCTTA CCAGTGCCCC AGCATGTACT GTGAGACCCC CATGGTTCAC 
CACATCCGGC GCTTTGTGCA CGGTAAAGGC TGCGTCCAGA TTGTACTGAA AGAGCTGGAC 
TCGCCTGTTC CTGGATATCA GCACACAATC CTCAACTACT CATGGTGTCG CATCTGCAAA 
CAGGTGACTC CTGTGGTCCC TTTGTCTAAT GACTCTTGGT CCATGTCCTT CGCCAAGTAC 
CTTGAGCTTC GTTTCTATGG TCATCAGTAC ACCCGTCGGG CTAACGCTGA GCCTTGTGGC 
CACTCTATCC ATAAAGATTA TCACCAGTAC TTCTCTTACA ACCAGATGGT GGCATCCTTC 
AGCTACATTC CAGTGAGGCT GTTAGAGATC TGTCTGCCTC CCCCGAAGAT CATCATCAGG 
AACCAAGGTC CATCGAAATC CAGCTTGCAG CAGGATCTCA AAGACTTCTC CCAGAAGGTG 
GCTCAGGTGT ACCTGGCTAT AGATGACCGT CTTACCTCCT TAAAAACGGA CACATTCAGC 
AAGACCAGAG AGGAAAAAAT GGAGGACATG TTTGCACAGA AAGATATGGA GGAATCAGAG 
CTTCGGGGCT GGATAGAGAA GTTACAAGTG CGTCTGCAGA CGAGTGTGAT TGACTCTTCA 
CAACAACTAC AGGCTGTTCT GGAGTCAGTG GTGGTCAAAA AACAGGGCTT GTGTGAAACC 
CTGCAGTCCT GGAACAACAG ACTTCAAGAA CTGTTCCAGC AAGAGAAAGG CAGAAAACGT 
CTATCTGTTC CTCCTAGCCC AGGCAGACAC AGACAACCTA CAACAGATGA GAGCAAGACT 
AGTGCTTTGG AGTCTTCTCC TCGTAACCCT TCCCCAGTGG TCCCAAATGG AGACAAAGAG 
GACCGTCATC TCAATACATT TCCATCAAGT TCAGGGTCCT CGTCGTTACT ACAGTTACCA 
TCTCCAGCTG AACAACCTTC AGATGTCATT ACAAGCGGAC CATCTTTCCC TGATCAGGAC 
TCTGTTAGCA ATCCAGAGGA CATGTTTGAT GGACACTTGC TTGGTTCCAA TGATAGTCAG 
GTAAAGGAAA AGTCCACCAT GAAAACAATC CTGGCCAACC TGTTACCTGG AAACAGCTAC 
AATCCCATCC CTTTTCCTTT TGACCCAGAC AAGCACTATC TGATGTATGA GCATGAGAGA 
GTTCCTATAG CCGTGTGTGA GAGAGAGCCC AGCTCCATAA TTGCCTTTGC ACTCAGCTGC 
AAAGAGTATA AAACTGCCCT TGAAGAGCTC ACAAAGACAA CAGCAAAGAC TGGAGGTGAT 
GACACTTCTC AGACCATCAG TGCTGGAGAG AGCAGAGTGA AGAACAGTCC AGCCAAACCC 
AGTGACAACA GCATGTCACA GCTGAGCCGC AGCAGCGTCG ACGCTGATCC ACTCAAGGAG 
CCTGAAAGTG CAGACAAACA GAAGAAGCAG ACAGGGAACC CACATATTGA GCTGCAGTTC 
TCCGATGCAA ATGCTAAGTT CTACTGCCGG ATCTACTATG CTGAAGAGTT CCATAAAATG 
CGGGAGGAGA TCATGGAGAG CACAGAGGAT GATTTTGTGC GATCTCTTTC CCACTGTGTC 
AACTGGCAGG CGCGTGGTGG GAAATCTGGT GCAGTTTTCT ATGCCACTGA AGATGATCGG 
TTTATTCTGA AGCAGATGCC CAGATTAGAG GTCCAGTCCT TTTTAGACTT TGCACCCCAC 
TACTTTACGT ACATCACAGG AGCGGTTCAC CTAAAGCGAC CCACAGCACT TGCTAAGATT 
CTGGGAGTGT ACCGCATCGG CTACAAGAAC TCCCAGAACA ACACTGAGAA GAAGCTTGAC 
CTGCTTGTGA TGGAAAACCT TTTCTATGGA CGAAAGATGG CACAGGTGTT TGACCTGAAA 
GGATCGCTGA GAAACAGGAA CGTGAAGACG GACCAGGGAA AGGAGAGCTG TGAGGTGGTG 
CTCCTAGATG AGAACCTGCT CAAACTGGTG CATGACAATC CCCTTTATAT TCGCTCGCAC 
TGCAAGGCCA TCCTGCGTGC TGCCATCCAC AGCGACGCGC TCTTCCTGTC CAGCCACCTC 
ATCATTGATT ACTCCCTGCT GGTGGGCCGA GACGATTCCA CAGATGAGCT AGTTGTGGGG 
ATCATAGATT ATATCCGGAC TTTCACTTGG GATAAAAAGC TGGAGATGGT GGTCAAATCT 
ACTGGGATTC TTGGAGGTCA AGGTAAAATG CCCACAGTGG TGTCACCAGA GCTGTATCGA 
ACACGTTTCT GTGAGGCCAT GGATAAATAC TTCCTTATGG TCCCTGATCA CTGGAGTGGA 
CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304724.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302999.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.

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CloneID	ODa40125
Clone ID Related Accession (Same CDS sequence)	XM_009304724.3 , XM_009304724.2
Accession Version	XM_009304724.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG CGGAGTGGGA AGAGGGTAGA 
CGGAAATCAG AAGCTCCTCT GGGCAGCCAC GATCCCAGAA CAGCTGTCCA GCTTCGCACA 
GCTCTCAAGA GGCTCAAAGA GATCATGGAA GGGAAAAGTC AGGACAGTGA TCTGAAACAG 
TACTGGATGC CAGATAGCCA GTGTAAGGAG TGCTATGACT GCAATGAGAA ATTCACAACC 
TTCCGACGCC GTCACCATTG TCGACTATGT GGACAAATAT TCTGCAGCCG ATGCTGCAAC 
CAGGAAATTC CTGGCAAGTT CATGGGCTAC ACGGGCGATT TAAGGGCTTG TACATACTGT 
CGTAAGATAG CATTAAGCTA TGCTCACTCA ACAGACTCAA GCTCCATTGG AGAAGATCTA 
AGTGCTTTGT CGGACTCGCC TTGCTCCGTC TGTGTTCTGG AGCCCACTGA ACCACGCACA 
CCTGTAGGAG GTCGCAAAGC CAGCCGCAAT ATCTTCCTGG AAGAGGACCT AGCCTGGCAA 
AGAAAAAATT CCATTGGGAT GAGGAAGAAT CATCAGGAAT CTCAGAACAG CGGTCTCAAT 
TCCAGACTGA CAGTGGTCCA AGAGGATATT GGAAAGTCAC CTGCCAGGAA GAGGTCAGCT 
AGTGTGACCA ATTTGTCTTT GGACCGTTCC GGCTCATCCA TGGTTCCTGC CTATGAGAGT 
TCGGTTAGTC CACAGAACAG CCGAGCTCTA CCCAAGACCG ACCACAGCGA AGAAGAGCGA 
AAGATCCTTC TAGATTCTTT TCAACTTAAA GATATATGGA AGAAGATTTG CCACAACACT 
ACTGGGATGG AGTTCCAGGA CCATCGGTAC TGGCTGCGCA CTTACCCCAA CTGCATTGTG 
GGTAAAGAGT TGGTCAACTG GCTTTTAAGA AATGGCACTA TTTCCACTAG GGCTCAAGCC 
ATAGCTATAG GACAGGCTTT GGTAGATGGC CGCTGGCTTG ACTGTGTAAC CCATCATGAT 
CAGATCTTCC GTGATGAGTA CGCCCTTTAT CGCCCCCTTC AGAGCACAGA GTTCTCCGAA 
ACTCCTTCTC CAGACAGTGA CAGTGTTAAT TCCCTGGAGG GACACTCTGA ACCGTCATGG 
TTTAAAGACA TCAAGTTTGA TGACAGTGAC ACCGAGCAGC TAGCAGATGA AAATGACTAT 
GTCACACCAA GCTCGTCCAG CCCCAGCAAA AGAACATCTG TCAGCAGTTT CCACTCTGCA 
GTAGACAGTG ACTCGGCCGC CTCCATCAGT CTAAACATGG AGCAGGACAA CGTCAATTTC 
CACATTAAGA AGCAGGCCAA GTACCCCCAT GTGCCTCCCT ACCCAGCTGA GCAAAAAAGT 
ATGGAGCCCC ATGACCCGTT CACCCCAGAA ACCGACATCC ATGCACCAAT GGAAGTCTTG 
CTCTCCGAAG ATGGAGGTCA GCATATATCC ATCAGTGATG CATTCATCAA AGAGTCTTTG 
TTCAACCGGA GGGTTGAGGA GAAAGCTAAA GAGATGCTCT TCACTCCTCT CGGCTGGCAT 
CACAGCTCTC TGGATCAGCT GCGGGAAGAG AATGGGGAAA AGAAAGCGAT GGAGCGGTTG 
CTGTCAGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AACTGCTGTA CAGTGAATCA 
CTGTCTCTGT CGTGGCGTGA CATCATCGTA CCTGTGGTGA GGCAGGTGGT GCAGACGGTG 
CGACCGGATG TGCGCAGCTG TGACGATGAT ATGGACATCC GTCAGTTTGT CCACATCAAG 
AAGATTCCAG GAGGAAAGAA GTTTGACTCT GCTGTAGTGA ATGGTTTTGT TTGCACAAAG 
AATATCGCAC ACAAAAAGAT GAACTCTTAC ATCAAAAACC CCAAGATCCT TCTTCTCAAA 
TGCTCCATTG AGTATCTATA TAGAGAGGAG ACCAAGTTCA CCTGCATTGA CCCAATTGTT 
TTACAGGAGC GTGAGTTTCT TAAGAACTAT GTTCAGAGGA TTGTTGATGT CCGACCAAAC 
TTAGTCCTGG TGGAGAAGAC TGTATCGCGA ATTGCTCAGG ACATGCTACT AGAGCACGGC 
ATTGCCATTG TGATTAATGT CAAACCTCAA GTACTGGACC GTGTGAGTCG AATGACTCAG 
GGAGATTTAG TCATTTCAAT GGATCAGCTT CTTACAAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACT TGCACTCCTT CCAGCTACCA AATAGTGAAG TGAAAACCCT GATGTTTTTT 
GAGGGCTGTC CTCCTCAGCT GGGCTGCACT ATTAAGCTCC GCGGAGCCTC AGAGTATGAG 
CTGGCACGGG TAAAAGAGAT CATCATTTTT ATGGTGTGTG TGGCATACCA CTCGCAGCTA 
GAGATCTCTT TCCTCATGGA TGAGTTCGCT ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT 
TTCCCATGTC TGCTGGAAAG CACCACTTTA CGGGAAGAGG ATGAAACTAG TGGGGAACGG 
GAAAGCCAAG ACAATGATGA GTCTACACTG GGAGATGACA ATCTCACACT TGTTCCAGGA 
GATGACTTTG AGACAGGATT TACAGGGACT ATCAAAGCCC ACAGTCGAGA GCCTTCCACC 
TCTGAATCTT CTCATAAAGA TGGAGAAAGC CCCAGAATAA ATAAAAATGC TTCAATTACC 
TCCTTCTCTG GAGGGGAGGA AGAAAATATA AAGACCTCCA CGCCACTTTC CTCCTTCTCT 
TCCACCTTTC CTCAGCCGGT GACACCACCT TTCCTGATAT CAGACGTAAA AGAAATGTCA 
CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG GAGAAGAACA AATATCTGGA GTCGACTCTG 
GTCCATCAGG ACAGCACAAG CTCTGAGACC TCCCTTCCCC CAACTCGGCT CTTTAGAGAC 
CCCTTACAGG ACGACACAGG CCTGTTTGTG ACAGAGCATG TAGCTTCTTC AGATGACCGC 
CTCAAATCCA TCTCTGCTTT ATTCAAACAG GAGCTAAAAG ACATCATCCT GTGCATTTCA 
CCCTTTATTA CCTTTCGAGA GCCTTTCTTG CTTACCCCAG CTGGACTGCG GTGTCCTAGC 
CGGGATTATT TCCCTGAACA GGTTTACCTT TCACCTCTGC TGAACAAGGA TTCAAAAGAA 
CTGGATGGAC GCCGCAAGAG GCAGCTGCTG AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG 
ACCAATGGTT CCGTTTCACA CCAGAGAGCC ATTCAGATCT TGCCCTGTCA CAAACTCACT 
AGTGCCCGAA TAGCAGAACA GGTAGGCAGC AGTCAGCAGC TGGCACGCAT GTTAGCGGAC 
TACCGTGCCC AAGGAGGACG TATCCGGCAA AGAGAGGGAA CACAATTTCG TGAAGCCCCT 
CCCCCAAAGG CGCCAATAAA GGCAGATAGT GAAGAAGATA AAGGTGCAGG AGTGATTGAC 
ATGACCTGGT CTACTAAGTT GGACTGTTTA AATCCAATCA ACCATCAGAG GCTCTGTGTT 
CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT AATGCCCCAA ACCCCTGCGT CAGTCCCTGG 
ATTGTTACAA TGGAGTTTTA TGGAAAGAAT GACTTGACTC TTGGTGTGTT TCTGGAGAGA 
TACTGTTTTA GACCATCTTA CCAGTGCCCC AGCATGTACT GTGAGACCCC CATGGTTCAC 
CACATCCGGC GCTTTGTGCA CGGTAAAGGC TGCGTCCAGA TTGTACTGAA AGAGCTGGAC 
TCGCCTGTTC CTGGATATCA GCACACAATC CTCAACTACT CATGGTGTCG CATCTGCAAA 
CAGGTGACTC CTGTGGTCCC TTTGTCTAAT GACTCTTGGT CCATGTCCTT CGCCAAGTAC 
CTTGAGCTTC GTTTCTATGG TCATCAGTAC ACCCGTCGGG CTAACGCTGA GCCTTGTGGC 
CACTCTATCC ATAAAGATTA TCACCAGTAC TTCTCTTACA ACCAGATGGT GGCATCCTTC 
AGCTACATTC CAGTGAGGCT GTTAGAGATC TGTCTGCCTC CCCCGAAGAT CATCATCAGG 
AACCAAGGTC CATCGAAATC CAGCTTGCAG CAGGATCTCA AAGACTTCTC CCAGAAGGTG 
GCTCAGGTGT ACCTGGCTAT AGATGACCGT CTTACCTCCT TAAAAACGGA CACATTCAGC 
AAGACCAGAG AGGAAAAAAT GGAGGACATG TTTGCACAGA AAGATATGGA GGAATCAGAG 
CTTCGGGGCT GGATAGAGAA GTTACAAGTG CGTCTGCAGA CGAGTGTGAT TGACTCTTCA 
CAACAACTAC AGGCTGTTCT GGAGTCAGTG GTGGTCAAAA AACAGGGCTT GTGTGAAACC 
CTGCAGTCCT GGAACAACAG ACTTCAAGAA CTGTTCCAGC AAGAGAAAGG CAGAAAACGT 
CTATCTGTTC CTCCTAGCCC AGGCAGACAC AGACAACCTA CAACAGATGA GAGCAAGACT 
AGTGCTTTGG AGTCTTCTCC TCGTAACCCT TCCCCAGTGG TCCCAAATGG AGACAAAGAG 
GACCGTCATC TCAATACATT TCCATCAAGT TCAGGGTCCT CGTCGTTACT ACAGTTACCA 
TCTCCAGCTG AACAACCTTC AGATGTCATT ACAAGCGGAC CATCTTTCCC TGATCAGGAC 
TCTGTTAGCA ATCCAGAGGA CATGTTTGAT GGACACTTGC TTGGTTCCAA TGATAGTCAG 
GTAAAGGAAA AGTCCACCAT GAAAACAATC CTGGCCAACC TGTTACCTGG AAACAGCTAC 
AATCCCATCC CTTTTCCTTT TGACCCAGAC AAGCACTATC TGATGTATGA GCATGAGAGA 
GTTCCTATAG CCGTGTGTGA GAGAGAGCCC AGCTCCATAA TTGCCTTTGC ACTCAGCTGC 
AAAGAGTATA AAACTGCCCT TGAAGAGCTC ACAAAGACAA CAGCAAAGAC TGGAGGTGAT 
GACACTTCTC AGACCATCAG TGCTGGAGAG AGCAGAGTGA AGAACAGTCC AGCCAAACCC 
AGTGACAACA GCATGTCACA GCTGAGCCGC AGCAGCGTCG ACGCTGATCC ACTCAAGGAG 
CCTGAAAGTG CAGACAAACA GAAGAAGCAG ACAGGGAACC CACATATTGA GCTGCAGTTC 
TCCGATGCAA ATGCTAAGTT CTACTGCCGG ATCTACTATG CTGAAGAGTT CCATAAAATG 
CGGGAGGAGA TCATGGAGAG CACAGAGGAT GATTTTGTGC GATCTCTTTC CCACTGTGTC 
AACTGGCAGG CGCGTGGTGG GAAATCTGGT GCAGTTTTCT ATGCCACTGA AGATGATCGG 
TTTATTCTGA AGCAGATGCC CAGATTAGAG GTCCAGTCCT TTTTAGACTT TGCACCCCAC 
TACTTTACGT ACATCACAGG AGCGGTTCAC CTAAAGCGAC CCACAGCACT TGCTAAGATT 
CTGGGAGTGT ACCGCATCGG CTACAAGAAC TCCCAGAACA ACACTGAGAA GAAGCTTGAC 
CTGCTTGTGA TGGAAAACCT TTTCTATGGA CGAAAGATGG CACAGGTGTT TGACCTGAAA 
GGATCGCTGA GAAACAGGAA CGTGAAGACG GACCAGGGAA AGGAGAGCTG TGAGGTGGTG 
CTCCTAGATG AGAACCTGCT CAAACTGGTG CATGACAATC CCCTTTATAT TCGCTCGCAC 
TGCAAGGCCA TCCTGCGTGC TGCCATCCAC AGCGACGCGC TCTTCCTGTC CAGCCACCTC 
ATCATTGATT ACTCCCTGCT GGTGGGCCGA GACGATTCCA CAGATGAGCT AGTTGTGGGG 
ATCATAGATT ATATCCGGAC TTTCACTTGG GATAAAAAGC TGGAGATGGT GGTCAAATCT 
ACTGGGATTC TTGGAGGTCA AGGTAAAATG CCCACAGTGG TGTCACCAGA GCTGTATCGA 
ACACGTTTCT GTGAGGCCAT GGATAAATAC TTCCTTATGG TCCCTGATCA CTGGAGTGGA 
CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302999.1
CDS	156..6473
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKSMEPHDPFTPETDIHAPMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304724.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304724.2

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG CGGAGTGGGA AGAGGGTAGA 
CGGAAATCAG AAGCTCCTCT GGGCAGCCAC GATCCCAGAA CAGCTGTCCA GCTTCGCACA 
GCTCTCAAGA GGCTCAAAGA GATCATGGAA GGGAAAAGTC AGGACAGTGA TCTGAAACAG 
TACTGGATGC CAGATAGCCA GTGTAAGGAG TGCTATGACT GCAATGAGAA ATTCACAACC 
TTCCGACGCC GTCACCATTG TCGACTATGT GGACAAATAT TCTGCAGCCG ATGCTGCAAC 
CAGGAAATTC CTGGCAAGTT CATGGGCTAC ACGGGCGATT TAAGGGCTTG TACATACTGT 
CGTAAGATAG CATTAAGCTA TGCTCACTCA ACAGACTCAA GCTCCATTGG AGAAGATCTA 
AGTGCTTTGT CGGACTCGCC TTGCTCCGTC TGTGTTCTGG AGCCCACTGA ACCACGCACA 
CCTGTAGGAG GTCGCAAAGC CAGCCGCAAT ATCTTCCTGG AAGAGGACCT AGCCTGGCAA 
AGAAAAAATT CCATTGGGAT GAGGAAGAAT CATCAGGAAT CTCAGAACAG CGGTCTCAAT 
TCCAGACTGA CAGTGGTCCA AGAGGATATT GGAAAGTCAC CTGCCAGGAA GAGGTCAGCT 
AGTGTGACCA ATTTGTCTTT GGACCGTTCC GGCTCATCCA TGGTTCCTGC CTATGAGAGT 
TCGGTTAGTC CACAGAACAG CCGAGCTCTA CCCAAGACCG ACCACAGCGA AGAAGAGCGA 
AAGATCCTTC TAGATTCTTT TCAACTTAAA GATATATGGA AGAAGATTTG CCACAACACT 
ACTGGGATGG AGTTCCAGGA CCATCGGTAC TGGCTGCGCA CTTACCCCAA CTGCATTGTG 
GGTAAAGAGT TGGTCAACTG GCTTTTAAGA AATGGCACTA TTTCCACTAG GGCTCAAGCC 
ATAGCTATAG GACAGGCTTT GGTAGATGGC CGCTGGCTTG ACTGTGTAAC CCATCATGAT 
CAGATCTTCC GTGATGAGTA CGCCCTTTAT CGCCCCCTTC AGAGCACAGA GTTCTCCGAA 
ACTCCTTCTC CAGACAGTGA CAGTGTTAAT TCCCTGGAGG GACACTCTGA ACCGTCATGG 
TTTAAAGACA TCAAGTTTGA TGACAGTGAC ACCGAGCAGC TAGCAGATGA AAATGACTAT 
GTCACACCAA GCTCGTCCAG CCCCAGCAAA AGAACATCTG TCAGCAGTTT CCACTCTGCA 
GTAGACAGTG ACTCGGCCGC CTCCATCAGT CTAAACATGG AGCAGGACAA CGTCAATTTC 
CACATTAAGA AGCAGGCCAA GTACCCCCAT GTGCCTCCCT ACCCAGCTGA GCAAAAAAGT 
ATGGAGCCCC ATGACCCGTT CACCCCAGAA ACCGACATCC ATGCACCAAT GGAAGTCTTG 
CTCTCCGAAG ATGGAGGTCA GCATATATCC ATCAGTGATG CATTCATCAA AGAGTCTTTG 
TTCAACCGGA GGGTTGAGGA GAAAGCTAAA GAGATGCTCT TCACTCCTCT CGGCTGGCAT 
CACAGCTCTC TGGATCAGCT GCGGGAAGAG AATGGGGAAA AGAAAGCGAT GGAGCGGTTG 
CTGTCAGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AACTGCTGTA CAGTGAATCA 
CTGTCTCTGT CGTGGCGTGA CATCATCGTA CCTGTGGTGA GGCAGGTGGT GCAGACGGTG 
CGACCGGATG TGCGCAGCTG TGACGATGAT ATGGACATCC GTCAGTTTGT CCACATCAAG 
AAGATTCCAG GAGGAAAGAA GTTTGACTCT GCTGTAGTGA ATGGTTTTGT TTGCACAAAG 
AATATCGCAC ACAAAAAGAT GAACTCTTAC ATCAAAAACC CCAAGATCCT TCTTCTCAAA 
TGCTCCATTG AGTATCTATA TAGAGAGGAG ACCAAGTTCA CCTGCATTGA CCCAATTGTT 
TTACAGGAGC GTGAGTTTCT TAAGAACTAT GTTCAGAGGA TTGTTGATGT CCGACCAAAC 
TTAGTCCTGG TGGAGAAGAC TGTATCGCGA ATTGCTCAGG ACATGCTACT AGAGCACGGC 
ATTGCCATTG TGATTAATGT CAAACCTCAA GTACTGGACC GTGTGAGTCG AATGACTCAG 
GGAGATTTAG TCATTTCAAT GGATCAGCTT CTTACAAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACT TGCACTCCTT CCAGCTACCA AATAGTGAAG TGAAAACCCT GATGTTTTTT 
GAGGGCTGTC CTCCTCAGCT GGGCTGCACT ATTAAGCTCC GCGGAGCCTC AGAGTATGAG 
CTGGCACGGG TAAAAGAGAT CATCATTTTT ATGGTGTGTG TGGCATACCA CTCGCAGCTA 
GAGATCTCTT TCCTCATGGA TGAGTTCGCT ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT 
TTCCCATGTC TGCTGGAAAG CACCACTTTA CGGGAAGAGG ATGAAACTAG TGGGGAACGG 
GAAAGCCAAG ACAATGATGA GTCTACACTG GGAGATGACA ATCTCACACT TGTTCCAGGA 
GATGACTTTG AGACAGGATT TACAGGGACT ATCAAAGCCC ACAGTCGAGA GCCTTCCACC 
TCTGAATCTT CTCATAAAGA TGGAGAAAGC CCCAGAATAA ATAAAAATGC TTCAATTACC 
TCCTTCTCTG GAGGGGAGGA AGAAAATATA AAGACCTCCA CGCCACTTTC CTCCTTCTCT 
TCCACCTTTC CTCAGCCGGT GACACCACCT TTCCTGATAT CAGACGTAAA AGAAATGTCA 
CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG GAGAAGAACA AATATCTGGA GTCGACTCTG 
GTCCATCAGG ACAGCACAAG CTCTGAGACC TCCCTTCCCC CAACTCGGCT CTTTAGAGAC 
CCCTTACAGG ACGACACAGG CCTGTTTGTG ACAGAGCATG TAGCTTCTTC AGATGACCGC 
CTCAAATCCA TCTCTGCTTT ATTCAAACAG GAGCTAAAAG ACATCATCCT GTGCATTTCA 
CCCTTTATTA CCTTTCGAGA GCCTTTCTTG CTTACCCCAG CTGGACTGCG GTGTCCTAGC 
CGGGATTATT TCCCTGAACA GGTTTACCTT TCACCTCTGC TGAACAAGGA TTCAAAAGAA 
CTGGATGGAC GCCGCAAGAG GCAGCTGCTG AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG 
ACCAATGGTT CCGTTTCACA CCAGAGAGCC ATTCAGATCT TGCCCTGTCA CAAACTCACT 
AGTGCCCGAA TAGCAGAACA GGTAGGCAGC AGTCAGCAGC TGGCACGCAT GTTAGCGGAC 
TACCGTGCCC AAGGAGGACG TATCCGGCAA AGAGAGGGAA CACAATTTCG TGAAGCCCCT 
CCCCCAAAGG CGCCAATAAA GGCAGATAGT GAAGAAGATA AAGGTGCAGG AGTGATTGAC 
ATGACCTGGT CTACTAAGTT GGACTGTTTA AATCCAATCA ACCATCAGAG GCTCTGTGTT 
CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT AATGCCCCAA ACCCCTGCGT CAGTCCCTGG 
ATTGTTACAA TGGAGTTTTA TGGAAAGAAT GACTTGACTC TTGGTGTGTT TCTGGAGAGA 
TACTGTTTTA GACCATCTTA CCAGTGCCCC AGCATGTACT GTGAGACCCC CATGGTTCAC 
CACATCCGGC GCTTTGTGCA CGGTAAAGGC TGCGTCCAGA TTGTACTGAA AGAGCTGGAC 
TCGCCTGTTC CTGGATATCA GCACACAATC CTCAACTACT CATGGTGTCG CATCTGCAAA 
CAGGTGACTC CTGTGGTCCC TTTGTCTAAT GACTCTTGGT CCATGTCCTT CGCCAAGTAC 
CTTGAGCTTC GTTTCTATGG TCATCAGTAC ACCCGTCGGG CTAACGCTGA GCCTTGTGGC 
CACTCTATCC ATAAAGATTA TCACCAGTAC TTCTCTTACA ACCAGATGGT GGCATCCTTC 
AGCTACATTC CAGTGAGGCT GTTAGAGATC TGTCTGCCTC CCCCGAAGAT CATCATCAGG 
AACCAAGGTC CATCGAAATC CAGCTTGCAG CAGGATCTCA AAGACTTCTC CCAGAAGGTG 
GCTCAGGTGT ACCTGGCTAT AGATGACCGT CTTACCTCCT TAAAAACGGA CACATTCAGC 
AAGACCAGAG AGGAAAAAAT GGAGGACATG TTTGCACAGA AAGATATGGA GGAATCAGAG 
CTTCGGGGCT GGATAGAGAA GTTACAAGTG CGTCTGCAGA CGAGTGTGAT TGACTCTTCA 
CAACAACTAC AGGCTGTTCT GGAGTCAGTG GTGGTCAAAA AACAGGGCTT GTGTGAAACC 
CTGCAGTCCT GGAACAACAG ACTTCAAGAA CTGTTCCAGC AAGAGAAAGG CAGAAAACGT 
CTATCTGTTC CTCCTAGCCC AGGCAGACAC AGACAACCTA CAACAGATGA GAGCAAGACT 
AGTGCTTTGG AGTCTTCTCC TCGTAACCCT TCCCCAGTGG TCCCAAATGG AGACAAAGAG 
GACCGTCATC TCAATACATT TCCATCAAGT TCAGGGTCCT CGTCGTTACT ACAGTTACCA 
TCTCCAGCTG AACAACCTTC AGATGTCATT ACAAGCGGAC CATCTTTCCC TGATCAGGAC 
TCTGTTAGCA ATCCAGAGGA CATGTTTGAT GGACACTTGC TTGGTTCCAA TGATAGTCAG 
GTAAAGGAAA AGTCCACCAT GAAAACAATC CTGGCCAACC TGTTACCTGG AAACAGCTAC 
AATCCCATCC CTTTTCCTTT TGACCCAGAC AAGCACTATC TGATGTATGA GCATGAGAGA 
GTTCCTATAG CCGTGTGTGA GAGAGAGCCC AGCTCCATAA TTGCCTTTGC ACTCAGCTGC 
AAAGAGTATA AAACTGCCCT TGAAGAGCTC ACAAAGACAA CAGCAAAGAC TGGAGGTGAT 
GACACTTCTC AGACCATCAG TGCTGGAGAG AGCAGAGTGA AGAACAGTCC AGCCAAACCC 
AGTGACAACA GCATGTCACA GCTGAGCCGC AGCAGCGTCG ACGCTGATCC ACTCAAGGAG 
CCTGAAAGTG CAGACAAACA GAAGAAGCAG ACAGGGAACC CACATATTGA GCTGCAGTTC 
TCCGATGCAA ATGCTAAGTT CTACTGCCGG ATCTACTATG CTGAAGAGTT CCATAAAATG 
CGGGAGGAGA TCATGGAGAG CACAGAGGAT GATTTTGTGC GATCTCTTTC CCACTGTGTC 
AACTGGCAGG CGCGTGGTGG GAAATCTGGT GCAGTTTTCT ATGCCACTGA AGATGATCGG 
TTTATTCTGA AGCAGATGCC CAGATTAGAG GTCCAGTCCT TTTTAGACTT TGCACCCCAC 
TACTTTACGT ACATCACAGG AGCGGTTCAC CTAAAGCGAC CCACAGCACT TGCTAAGATT 
CTGGGAGTGT ACCGCATCGG CTACAAGAAC TCCCAGAACA ACACTGAGAA GAAGCTTGAC 
CTGCTTGTGA TGGAAAACCT TTTCTATGGA CGAAAGATGG CACAGGTGTT TGACCTGAAA 
GGATCGCTGA GAAACAGGAA CGTGAAGACG GACCAGGGAA AGGAGAGCTG TGAGGTGGTG 
CTCCTAGATG AGAACCTGCT CAAACTGGTG CATGACAATC CCCTTTATAT TCGCTCGCAC 
TGCAAGGCCA TCCTGCGTGC TGCCATCCAC AGCGACGCGC TCTTCCTGTC CAGCCACCTC 
ATCATTGATT ACTCCCTGCT GGTGGGCCGA GACGATTCCA CAGATGAGCT AGTTGTGGGG 
ATCATAGATT ATATCCGGAC TTTCACTTGG GATAAAAAGC TGGAGATGGT GGTCAAATCT 
ACTGGGATTC TTGGAGGTCA AGGTAAAATG CCCACAGTGG TGTCACCAGA GCTGTATCGA 
ACACGTTTCT GTGAGGCCAT GGATAAATAC TTCCTTATGG TCCCTGATCA CTGGAGTGGA 
CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304725.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303000.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.

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CloneID	ODa40126
Clone ID Related Accession (Same CDS sequence)	XM_009304725.2 , XM_009304725.3
Accession Version	XM_009304725.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAT GGAAGTCTTG CTCTCCGAAG ATGGAGGTCA GCATATATCC 
ATCAGTGATG CATTCATCAA AGAGTCTTTG TTCAACCGGA GGGTTGAGGA GAAAGCTAAA 
GAGATGCTCT TCACTCCTCT CGGCTGGCAT CACAGCTCTC TGGATCAGCT GCGGGAAGAG 
AATGGGGAAA AGAAAGCGAT GGAGCGGTTG CTGTCAGCAA ATCACAGCCA CATGATGGCG 
CTGCTGCAGC AACTGCTGTA CAGTGAATCA CTGTCTCTGT CGTGGCGTGA CATCATCGTA 
CCTGTGGTGA GGCAGGTGGT GCAGACGGTG CGACCGGATG TGCGCAGCTG TGACGATGAT 
ATGGACATCC GTCAGTTTGT CCACATCAAG AAGATTCCAG GAGGAAAGAA GTTTGACTCT 
GCTGTAGTGA ATGGTTTTGT TTGCACAAAG AATATCGCAC ACAAAAAGAT GAACTCTTAC 
ATCAAAAACC CCAAGATCCT TCTTCTCAAA TGCTCCATTG AGTATCTATA TAGAGAGGAG 
ACCAAGTTCA CCTGCATTGA CCCAATTGTT TTACAGGAGC GTGAGTTTCT TAAGAACTAT 
GTTCAGAGGA TTGTTGATGT CCGACCAAAC TTAGTCCTGG TGGAGAAGAC TGTATCGCGA 
ATTGCTCAGG ACATGCTACT AGAGCACGGC ATTGCCATTG TGATTAATGT CAAACCTCAA 
GTACTGGACC GTGTGAGTCG AATGACTCAG GGAGATTTAG TCATTTCAAT GGATCAGCTT 
CTTACAAAAC CTCGTCTGGG TACCTGCCAC AAGTTTTACT TGCACTCCTT CCAGCTACCA 
AATAGTGAAG TGAAAACCCT GATGTTTTTT GAGGGCTGTC CTCCTCAGCT GGGCTGCACT 
ATTAAGCTCC GCGGAGCCTC AGAGTATGAG CTGGCACGGG TAAAAGAGAT CATCATTTTT 
ATGGTGTGTG TGGCATACCA CTCGCAGCTA GAGATCTCTT TCCTCATGGA TGAGTTCGCT 
ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT TTCCCATGTC TGCTGGAAAG CACCACTTTA 
CGGGAAGAGG ATGAAACTAG TGGGGAACGG GAAAGCCAAG ACAATGATGA GTCTACACTG 
GGAGATGACA ATCTCACACT TGTTCCAGGA GATGACTTTG AGACAGGATT TACAGGGACT 
ATCAAAGCCC ACAGTCGAGA GCCTTCCACC TCTGAATCTT CTCATAAAGA TGGAGAAAGC 
CCCAGAATAA ATAAAAATGC TTCAATTACC TCCTTCTCTG GAGGGGAGGA AGAAAATATA 
AAGACCTCCA CGCCACTTTC CTCCTTCTCT TCCACCTTTC CTCAGCCGGT GACACCACCT 
TTCCTGATAT CAGACGTAAA AGAAATGTCA CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG 
GAGAAGAACA AATATCTGGA GTCGACTCTG GTCCATCAGG ACAGCACAAG CTCTGAGACC 
TCCCTTCCCC CAACTCGGCT CTTTAGAGAC CCCTTACAGG ACGACACAGG CCTGTTTGTG 
ACAGAGCATG TAGCTTCTTC AGATGACCGC CTCAAATCCA TCTCTGCTTT ATTCAAACAG 
GAGCTAAAAG ACATCATCCT GTGCATTTCA CCCTTTATTA CCTTTCGAGA GCCTTTCTTG 
CTTACCCCAG CTGGACTGCG GTGTCCTAGC CGGGATTATT TCCCTGAACA GGTTTACCTT 
TCACCTCTGC TGAACAAGGA TTCAAAAGAA CTGGATGGAC GCCGCAAGAG GCAGCTGCTG 
AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG ACCAATGGTT CCGTTTCACA CCAGAGAGCC 
ATTCAGATCT TGCCCTGTCA CAAACTCACT AGTGCCCGAA TAGCAGAACA GGTAGGCAGC 
AGTCAGCAGC TGGCACGCAT GTTAGCGGAC TACCGTGCCC AAGGAGGACG TATCCGGCAA 
AGAGAGGGAA CACAATTTCG TGAAGCCCCT CCCCCAAAGG CGCCAATAAA GGCAGATAGT 
GAAGAAGATA AAGGTGCAGG AGTGATTGAC ATGACCTGGT CTACTAAGTT GGACTGTTTA 
AATCCAATCA ACCATCAGAG GCTCTGTGTT CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT 
AATGCCCCAA ACCCCTGCGT CAGTCCCTGG ATTGTTACAA TGGAGTTTTA TGGAAAGAAT 
GACTTGACTC TTGGTGTGTT TCTGGAGAGA TACTGTTTTA GACCATCTTA CCAGTGCCCC 
AGCATGTACT GTGAGACCCC CATGGTTCAC CACATCCGGC GCTTTGTGCA CGGTAAAGGC 
TGCGTCCAGA TTGTACTGAA AGAGCTGGAC TCGCCTGTTC CTGGATATCA GCACACAATC 
CTCAACTACT CATGGTGTCG CATCTGCAAA CAGGTGACTC CTGTGGTCCC TTTGTCTAAT 
GACTCTTGGT CCATGTCCTT CGCCAAGTAC CTTGAGCTTC GTTTCTATGG TCATCAGTAC 
ACCCGTCGGG CTAACGCTGA GCCTTGTGGC CACTCTATCC ATAAAGATTA TCACCAGTAC 
TTCTCTTACA ACCAGATGGT GGCATCCTTC AGCTACATTC CAGTGAGGCT GTTAGAGATC 
TGTCTGCCTC CCCCGAAGAT CATCATCAGG AACCAAGGTC CATCGAAATC CAGCTTGCAG 
CAGGATCTCA AAGACTTCTC CCAGAAGGTG GCTCAGGTGT ACCTGGCTAT AGATGACCGT 
CTTACCTCCT TAAAAACGGA CACATTCAGC AAGACCAGAG AGGAAAAAAT GGAGGACATG 
TTTGCACAGA AAGATATGGA GGAATCAGAG CTTCGGGGCT GGATAGAGAA GTTACAAGTG 
CGTCTGCAGA CGAGTGTGAT TGACTCTTCA CAACAACTAC AGGCTGTTCT GGAGTCAGTG 
GTGGTCAAAA AACAGGGCTT GTGTGAAACC CTGCAGTCCT GGAACAACAG ACTTCAAGAA 
CTGTTCCAGC AAGAGAAAGG CAGAAAACGT CTATCTGTTC CTCCTAGCCC AGGCAGACAC 
AGACAACCTA CAACAGATGA GAGCAAGACT AGTGCTTTGG AGTCTTCTCC TCGTAACCCT 
TCCCCAGTGG TCCCAAATGG AGACAAAGAG GACCGTCATC TCAATACATT TCCATCAAGT 
TCAGGGTCCT CGTCGTTACT ACAGTTACCA TCTCCAGCTG AACAACCTTC AGATGTCATT 
ACAAGCGGAC CATCTTTCCC TGATCAGGAC TCTGTTAGCA ATCCAGAGGA CATGTTTGAT 
GGACACTTGC TTGGTTCCAA TGATAGTCAG GTAAAGGAAA AGTCCACCAT GAAAACAATC 
CTGGCCAACC TGTTACCTGG AAACAGCTAC AATCCCATCC CTTTTCCTTT TGACCCAGAC 
AAGCACTATC TGATGTATGA GCATGAGAGA GTTCCTATAG CCGTGTGTGA GAGAGAGCCC 
AGCTCCATAA TTGCCTTTGC ACTCAGCTGC AAAGAGTATA AAACTGCCCT TGAAGAGCTC 
ACAAAGACAA CAGCAAAGAC TGGAGGTGAT GACACTTCTC AGACCATCAG TGCTGGAGAG 
AGCAGAGTGA AGAACAGTCC AGCCAAACCC AGTGACAACA GCATGTCACA GCTGAGCCGC 
AGCAGCGTCG ACGCTGATCC ACTCAAGGAG CCTGAAAGTG CAGACAAACA GAAGAAGCAG 
ACAGGGAACC CACATATTGA GCTGCAGTTC TCCGATGCAA ATGCTAAGTT CTACTGCCGG 
ATCTACTATG CTGAAGAGTT CCATAAAATG CGGGAGGAGA TCATGGAGAG CACAGAGGAT 
GATTTTGTGC GATCTCTTTC CCACTGTGTC AACTGGCAGG CGCGTGGTGG GAAATCTGGT 
GCAGTTTTCT ATGCCACTGA AGATGATCGG TTTATTCTGA AGCAGATGCC CAGATTAGAG 
GTCCAGTCCT TTTTAGACTT TGCACCCCAC TACTTTACGT ACATCACAGG AGCGGTTCAC 
CTAAAGCGAC CCACAGCACT TGCTAAGATT CTGGGAGTGT ACCGCATCGG CTACAAGAAC 
TCCCAGAACA ACACTGAGAA GAAGCTTGAC CTGCTTGTGA TGGAAAACCT TTTCTATGGA 
CGAAAGATGG CACAGGTGTT TGACCTGAAA GGATCGCTGA GAAACAGGAA CGTGAAGACG 
GACCAGGGAA AGGAGAGCTG TGAGGTGGTG CTCCTAGATG AGAACCTGCT CAAACTGGTG 
CATGACAATC CCCTTTATAT TCGCTCGCAC TGCAAGGCCA TCCTGCGTGC TGCCATCCAC 
AGCGACGCGC TCTTCCTGTC CAGCCACCTC ATCATTGATT ACTCCCTGCT GGTGGGCCGA 
GACGATTCCA CAGATGAGCT AGTTGTGGGG ATCATAGATT ATATCCGGAC TTTCACTTGG 
GATAAAAAGC TGGAGATGGT GGTCAAATCT ACTGGGATTC TTGGAGGTCA AGGTAAAATG 
CCCACAGTGG TGTCACCAGA GCTGTATCGA ACACGTTTCT GTGAGGCCAT GGATAAATAC 
TTCCTTATGG TCCCTGATCA CTGGAGTGGA CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303000.1
CDS	156..6443
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQSHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304725.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304725.2

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAT GGAAGTCTTG CTCTCCGAAG ATGGAGGTCA GCATATATCC 
ATCAGTGATG CATTCATCAA AGAGTCTTTG TTCAACCGGA GGGTTGAGGA GAAAGCTAAA 
GAGATGCTCT TCACTCCTCT CGGCTGGCAT CACAGCTCTC TGGATCAGCT GCGGGAAGAG 
AATGGGGAAA AGAAAGCGAT GGAGCGGTTG CTGTCAGCAA ATCACAGCCA CATGATGGCG 
CTGCTGCAGC AACTGCTGTA CAGTGAATCA CTGTCTCTGT CGTGGCGTGA CATCATCGTA 
CCTGTGGTGA GGCAGGTGGT GCAGACGGTG CGACCGGATG TGCGCAGCTG TGACGATGAT 
ATGGACATCC GTCAGTTTGT CCACATCAAG AAGATTCCAG GAGGAAAGAA GTTTGACTCT 
GCTGTAGTGA ATGGTTTTGT TTGCACAAAG AATATCGCAC ACAAAAAGAT GAACTCTTAC 
ATCAAAAACC CCAAGATCCT TCTTCTCAAA TGCTCCATTG AGTATCTATA TAGAGAGGAG 
ACCAAGTTCA CCTGCATTGA CCCAATTGTT TTACAGGAGC GTGAGTTTCT TAAGAACTAT 
GTTCAGAGGA TTGTTGATGT CCGACCAAAC TTAGTCCTGG TGGAGAAGAC TGTATCGCGA 
ATTGCTCAGG ACATGCTACT AGAGCACGGC ATTGCCATTG TGATTAATGT CAAACCTCAA 
GTACTGGACC GTGTGAGTCG AATGACTCAG GGAGATTTAG TCATTTCAAT GGATCAGCTT 
CTTACAAAAC CTCGTCTGGG TACCTGCCAC AAGTTTTACT TGCACTCCTT CCAGCTACCA 
AATAGTGAAG TGAAAACCCT GATGTTTTTT GAGGGCTGTC CTCCTCAGCT GGGCTGCACT 
ATTAAGCTCC GCGGAGCCTC AGAGTATGAG CTGGCACGGG TAAAAGAGAT CATCATTTTT 
ATGGTGTGTG TGGCATACCA CTCGCAGCTA GAGATCTCTT TCCTCATGGA TGAGTTCGCT 
ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT TTCCCATGTC TGCTGGAAAG CACCACTTTA 
CGGGAAGAGG ATGAAACTAG TGGGGAACGG GAAAGCCAAG ACAATGATGA GTCTACACTG 
GGAGATGACA ATCTCACACT TGTTCCAGGA GATGACTTTG AGACAGGATT TACAGGGACT 
ATCAAAGCCC ACAGTCGAGA GCCTTCCACC TCTGAATCTT CTCATAAAGA TGGAGAAAGC 
CCCAGAATAA ATAAAAATGC TTCAATTACC TCCTTCTCTG GAGGGGAGGA AGAAAATATA 
AAGACCTCCA CGCCACTTTC CTCCTTCTCT TCCACCTTTC CTCAGCCGGT GACACCACCT 
TTCCTGATAT CAGACGTAAA AGAAATGTCA CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG 
GAGAAGAACA AATATCTGGA GTCGACTCTG GTCCATCAGG ACAGCACAAG CTCTGAGACC 
TCCCTTCCCC CAACTCGGCT CTTTAGAGAC CCCTTACAGG ACGACACAGG CCTGTTTGTG 
ACAGAGCATG TAGCTTCTTC AGATGACCGC CTCAAATCCA TCTCTGCTTT ATTCAAACAG 
GAGCTAAAAG ACATCATCCT GTGCATTTCA CCCTTTATTA CCTTTCGAGA GCCTTTCTTG 
CTTACCCCAG CTGGACTGCG GTGTCCTAGC CGGGATTATT TCCCTGAACA GGTTTACCTT 
TCACCTCTGC TGAACAAGGA TTCAAAAGAA CTGGATGGAC GCCGCAAGAG GCAGCTGCTG 
AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG ACCAATGGTT CCGTTTCACA CCAGAGAGCC 
ATTCAGATCT TGCCCTGTCA CAAACTCACT AGTGCCCGAA TAGCAGAACA GGTAGGCAGC 
AGTCAGCAGC TGGCACGCAT GTTAGCGGAC TACCGTGCCC AAGGAGGACG TATCCGGCAA 
AGAGAGGGAA CACAATTTCG TGAAGCCCCT CCCCCAAAGG CGCCAATAAA GGCAGATAGT 
GAAGAAGATA AAGGTGCAGG AGTGATTGAC ATGACCTGGT CTACTAAGTT GGACTGTTTA 
AATCCAATCA ACCATCAGAG GCTCTGTGTT CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT 
AATGCCCCAA ACCCCTGCGT CAGTCCCTGG ATTGTTACAA TGGAGTTTTA TGGAAAGAAT 
GACTTGACTC TTGGTGTGTT TCTGGAGAGA TACTGTTTTA GACCATCTTA CCAGTGCCCC 
AGCATGTACT GTGAGACCCC CATGGTTCAC CACATCCGGC GCTTTGTGCA CGGTAAAGGC 
TGCGTCCAGA TTGTACTGAA AGAGCTGGAC TCGCCTGTTC CTGGATATCA GCACACAATC 
CTCAACTACT CATGGTGTCG CATCTGCAAA CAGGTGACTC CTGTGGTCCC TTTGTCTAAT 
GACTCTTGGT CCATGTCCTT CGCCAAGTAC CTTGAGCTTC GTTTCTATGG TCATCAGTAC 
ACCCGTCGGG CTAACGCTGA GCCTTGTGGC CACTCTATCC ATAAAGATTA TCACCAGTAC 
TTCTCTTACA ACCAGATGGT GGCATCCTTC AGCTACATTC CAGTGAGGCT GTTAGAGATC 
TGTCTGCCTC CCCCGAAGAT CATCATCAGG AACCAAGGTC CATCGAAATC CAGCTTGCAG 
CAGGATCTCA AAGACTTCTC CCAGAAGGTG GCTCAGGTGT ACCTGGCTAT AGATGACCGT 
CTTACCTCCT TAAAAACGGA CACATTCAGC AAGACCAGAG AGGAAAAAAT GGAGGACATG 
TTTGCACAGA AAGATATGGA GGAATCAGAG CTTCGGGGCT GGATAGAGAA GTTACAAGTG 
CGTCTGCAGA CGAGTGTGAT TGACTCTTCA CAACAACTAC AGGCTGTTCT GGAGTCAGTG 
GTGGTCAAAA AACAGGGCTT GTGTGAAACC CTGCAGTCCT GGAACAACAG ACTTCAAGAA 
CTGTTCCAGC AAGAGAAAGG CAGAAAACGT CTATCTGTTC CTCCTAGCCC AGGCAGACAC 
AGACAACCTA CAACAGATGA GAGCAAGACT AGTGCTTTGG AGTCTTCTCC TCGTAACCCT 
TCCCCAGTGG TCCCAAATGG AGACAAAGAG GACCGTCATC TCAATACATT TCCATCAAGT 
TCAGGGTCCT CGTCGTTACT ACAGTTACCA TCTCCAGCTG AACAACCTTC AGATGTCATT 
ACAAGCGGAC CATCTTTCCC TGATCAGGAC TCTGTTAGCA ATCCAGAGGA CATGTTTGAT 
GGACACTTGC TTGGTTCCAA TGATAGTCAG GTAAAGGAAA AGTCCACCAT GAAAACAATC 
CTGGCCAACC TGTTACCTGG AAACAGCTAC AATCCCATCC CTTTTCCTTT TGACCCAGAC 
AAGCACTATC TGATGTATGA GCATGAGAGA GTTCCTATAG CCGTGTGTGA GAGAGAGCCC 
AGCTCCATAA TTGCCTTTGC ACTCAGCTGC AAAGAGTATA AAACTGCCCT TGAAGAGCTC 
ACAAAGACAA CAGCAAAGAC TGGAGGTGAT GACACTTCTC AGACCATCAG TGCTGGAGAG 
AGCAGAGTGA AGAACAGTCC AGCCAAACCC AGTGACAACA GCATGTCACA GCTGAGCCGC 
AGCAGCGTCG ACGCTGATCC ACTCAAGGAG CCTGAAAGTG CAGACAAACA GAAGAAGCAG 
ACAGGGAACC CACATATTGA GCTGCAGTTC TCCGATGCAA ATGCTAAGTT CTACTGCCGG 
ATCTACTATG CTGAAGAGTT CCATAAAATG CGGGAGGAGA TCATGGAGAG CACAGAGGAT 
GATTTTGTGC GATCTCTTTC CCACTGTGTC AACTGGCAGG CGCGTGGTGG GAAATCTGGT 
GCAGTTTTCT ATGCCACTGA AGATGATCGG TTTATTCTGA AGCAGATGCC CAGATTAGAG 
GTCCAGTCCT TTTTAGACTT TGCACCCCAC TACTTTACGT ACATCACAGG AGCGGTTCAC 
CTAAAGCGAC CCACAGCACT TGCTAAGATT CTGGGAGTGT ACCGCATCGG CTACAAGAAC 
TCCCAGAACA ACACTGAGAA GAAGCTTGAC CTGCTTGTGA TGGAAAACCT TTTCTATGGA 
CGAAAGATGG CACAGGTGTT TGACCTGAAA GGATCGCTGA GAAACAGGAA CGTGAAGACG 
GACCAGGGAA AGGAGAGCTG TGAGGTGGTG CTCCTAGATG AGAACCTGCT CAAACTGGTG 
CATGACAATC CCCTTTATAT TCGCTCGCAC TGCAAGGCCA TCCTGCGTGC TGCCATCCAC 
AGCGACGCGC TCTTCCTGTC CAGCCACCTC ATCATTGATT ACTCCCTGCT GGTGGGCCGA 
GACGATTCCA CAGATGAGCT AGTTGTGGGG ATCATAGATT ATATCCGGAC TTTCACTTGG 
GATAAAAAGC TGGAGATGGT GGTCAAATCT ACTGGGATTC TTGGAGGTCA AGGTAAAATG 
CCCACAGTGG TGTCACCAGA GCTGTATCGA ACACGTTTCT GTGAGGCCAT GGATAAATAC 
TTCCTTATGG TCCCTGATCA CTGGAGTGGA CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_009304725.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303000.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.

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CloneID	ODa40126
Clone ID Related Accession (Same CDS sequence)	XM_009304725.2 , XM_009304725.3
Accession Version	XM_009304725.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAT GGAAGTCTTG CTCTCCGAAG ATGGAGGTCA GCATATATCC 
ATCAGTGATG CATTCATCAA AGAGTCTTTG TTCAACCGGA GGGTTGAGGA GAAAGCTAAA 
GAGATGCTCT TCACTCCTCT CGGCTGGCAT CACAGCTCTC TGGATCAGCT GCGGGAAGAG 
AATGGGGAAA AGAAAGCGAT GGAGCGGTTG CTGTCAGCAA ATCACAGCCA CATGATGGCG 
CTGCTGCAGC AACTGCTGTA CAGTGAATCA CTGTCTCTGT CGTGGCGTGA CATCATCGTA 
CCTGTGGTGA GGCAGGTGGT GCAGACGGTG CGACCGGATG TGCGCAGCTG TGACGATGAT 
ATGGACATCC GTCAGTTTGT CCACATCAAG AAGATTCCAG GAGGAAAGAA GTTTGACTCT 
GCTGTAGTGA ATGGTTTTGT TTGCACAAAG AATATCGCAC ACAAAAAGAT GAACTCTTAC 
ATCAAAAACC CCAAGATCCT TCTTCTCAAA TGCTCCATTG AGTATCTATA TAGAGAGGAG 
ACCAAGTTCA CCTGCATTGA CCCAATTGTT TTACAGGAGC GTGAGTTTCT TAAGAACTAT 
GTTCAGAGGA TTGTTGATGT CCGACCAAAC TTAGTCCTGG TGGAGAAGAC TGTATCGCGA 
ATTGCTCAGG ACATGCTACT AGAGCACGGC ATTGCCATTG TGATTAATGT CAAACCTCAA 
GTACTGGACC GTGTGAGTCG AATGACTCAG GGAGATTTAG TCATTTCAAT GGATCAGCTT 
CTTACAAAAC CTCGTCTGGG TACCTGCCAC AAGTTTTACT TGCACTCCTT CCAGCTACCA 
AATAGTGAAG TGAAAACCCT GATGTTTTTT GAGGGCTGTC CTCCTCAGCT GGGCTGCACT 
ATTAAGCTCC GCGGAGCCTC AGAGTATGAG CTGGCACGGG TAAAAGAGAT CATCATTTTT 
ATGGTGTGTG TGGCATACCA CTCGCAGCTA GAGATCTCTT TCCTCATGGA TGAGTTCGCT 
ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT TTCCCATGTC TGCTGGAAAG CACCACTTTA 
CGGGAAGAGG ATGAAACTAG TGGGGAACGG GAAAGCCAAG ACAATGATGA GTCTACACTG 
GGAGATGACA ATCTCACACT TGTTCCAGGA GATGACTTTG AGACAGGATT TACAGGGACT 
ATCAAAGCCC ACAGTCGAGA GCCTTCCACC TCTGAATCTT CTCATAAAGA TGGAGAAAGC 
CCCAGAATAA ATAAAAATGC TTCAATTACC TCCTTCTCTG GAGGGGAGGA AGAAAATATA 
AAGACCTCCA CGCCACTTTC CTCCTTCTCT TCCACCTTTC CTCAGCCGGT GACACCACCT 
TTCCTGATAT CAGACGTAAA AGAAATGTCA CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG 
GAGAAGAACA AATATCTGGA GTCGACTCTG GTCCATCAGG ACAGCACAAG CTCTGAGACC 
TCCCTTCCCC CAACTCGGCT CTTTAGAGAC CCCTTACAGG ACGACACAGG CCTGTTTGTG 
ACAGAGCATG TAGCTTCTTC AGATGACCGC CTCAAATCCA TCTCTGCTTT ATTCAAACAG 
GAGCTAAAAG ACATCATCCT GTGCATTTCA CCCTTTATTA CCTTTCGAGA GCCTTTCTTG 
CTTACCCCAG CTGGACTGCG GTGTCCTAGC CGGGATTATT TCCCTGAACA GGTTTACCTT 
TCACCTCTGC TGAACAAGGA TTCAAAAGAA CTGGATGGAC GCCGCAAGAG GCAGCTGCTG 
AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG ACCAATGGTT CCGTTTCACA CCAGAGAGCC 
ATTCAGATCT TGCCCTGTCA CAAACTCACT AGTGCCCGAA TAGCAGAACA GGTAGGCAGC 
AGTCAGCAGC TGGCACGCAT GTTAGCGGAC TACCGTGCCC AAGGAGGACG TATCCGGCAA 
AGAGAGGGAA CACAATTTCG TGAAGCCCCT CCCCCAAAGG CGCCAATAAA GGCAGATAGT 
GAAGAAGATA AAGGTGCAGG AGTGATTGAC ATGACCTGGT CTACTAAGTT GGACTGTTTA 
AATCCAATCA ACCATCAGAG GCTCTGTGTT CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT 
AATGCCCCAA ACCCCTGCGT CAGTCCCTGG ATTGTTACAA TGGAGTTTTA TGGAAAGAAT 
GACTTGACTC TTGGTGTGTT TCTGGAGAGA TACTGTTTTA GACCATCTTA CCAGTGCCCC 
AGCATGTACT GTGAGACCCC CATGGTTCAC CACATCCGGC GCTTTGTGCA CGGTAAAGGC 
TGCGTCCAGA TTGTACTGAA AGAGCTGGAC TCGCCTGTTC CTGGATATCA GCACACAATC 
CTCAACTACT CATGGTGTCG CATCTGCAAA CAGGTGACTC CTGTGGTCCC TTTGTCTAAT 
GACTCTTGGT CCATGTCCTT CGCCAAGTAC CTTGAGCTTC GTTTCTATGG TCATCAGTAC 
ACCCGTCGGG CTAACGCTGA GCCTTGTGGC CACTCTATCC ATAAAGATTA TCACCAGTAC 
TTCTCTTACA ACCAGATGGT GGCATCCTTC AGCTACATTC CAGTGAGGCT GTTAGAGATC 
TGTCTGCCTC CCCCGAAGAT CATCATCAGG AACCAAGGTC CATCGAAATC CAGCTTGCAG 
CAGGATCTCA AAGACTTCTC CCAGAAGGTG GCTCAGGTGT ACCTGGCTAT AGATGACCGT 
CTTACCTCCT TAAAAACGGA CACATTCAGC AAGACCAGAG AGGAAAAAAT GGAGGACATG 
TTTGCACAGA AAGATATGGA GGAATCAGAG CTTCGGGGCT GGATAGAGAA GTTACAAGTG 
CGTCTGCAGA CGAGTGTGAT TGACTCTTCA CAACAACTAC AGGCTGTTCT GGAGTCAGTG 
GTGGTCAAAA AACAGGGCTT GTGTGAAACC CTGCAGTCCT GGAACAACAG ACTTCAAGAA 
CTGTTCCAGC AAGAGAAAGG CAGAAAACGT CTATCTGTTC CTCCTAGCCC AGGCAGACAC 
AGACAACCTA CAACAGATGA GAGCAAGACT AGTGCTTTGG AGTCTTCTCC TCGTAACCCT 
TCCCCAGTGG TCCCAAATGG AGACAAAGAG GACCGTCATC TCAATACATT TCCATCAAGT 
TCAGGGTCCT CGTCGTTACT ACAGTTACCA TCTCCAGCTG AACAACCTTC AGATGTCATT 
ACAAGCGGAC CATCTTTCCC TGATCAGGAC TCTGTTAGCA ATCCAGAGGA CATGTTTGAT 
GGACACTTGC TTGGTTCCAA TGATAGTCAG GTAAAGGAAA AGTCCACCAT GAAAACAATC 
CTGGCCAACC TGTTACCTGG AAACAGCTAC AATCCCATCC CTTTTCCTTT TGACCCAGAC 
AAGCACTATC TGATGTATGA GCATGAGAGA GTTCCTATAG CCGTGTGTGA GAGAGAGCCC 
AGCTCCATAA TTGCCTTTGC ACTCAGCTGC AAAGAGTATA AAACTGCCCT TGAAGAGCTC 
ACAAAGACAA CAGCAAAGAC TGGAGGTGAT GACACTTCTC AGACCATCAG TGCTGGAGAG 
AGCAGAGTGA AGAACAGTCC AGCCAAACCC AGTGACAACA GCATGTCACA GCTGAGCCGC 
AGCAGCGTCG ACGCTGATCC ACTCAAGGAG CCTGAAAGTG CAGACAAACA GAAGAAGCAG 
ACAGGGAACC CACATATTGA GCTGCAGTTC TCCGATGCAA ATGCTAAGTT CTACTGCCGG 
ATCTACTATG CTGAAGAGTT CCATAAAATG CGGGAGGAGA TCATGGAGAG CACAGAGGAT 
GATTTTGTGC GATCTCTTTC CCACTGTGTC AACTGGCAGG CGCGTGGTGG GAAATCTGGT 
GCAGTTTTCT ATGCCACTGA AGATGATCGG TTTATTCTGA AGCAGATGCC CAGATTAGAG 
GTCCAGTCCT TTTTAGACTT TGCACCCCAC TACTTTACGT ACATCACAGG AGCGGTTCAC 
CTAAAGCGAC CCACAGCACT TGCTAAGATT CTGGGAGTGT ACCGCATCGG CTACAAGAAC 
TCCCAGAACA ACACTGAGAA GAAGCTTGAC CTGCTTGTGA TGGAAAACCT TTTCTATGGA 
CGAAAGATGG CACAGGTGTT TGACCTGAAA GGATCGCTGA GAAACAGGAA CGTGAAGACG 
GACCAGGGAA AGGAGAGCTG TGAGGTGGTG CTCCTAGATG AGAACCTGCT CAAACTGGTG 
CATGACAATC CCCTTTATAT TCGCTCGCAC TGCAAGGCCA TCCTGCGTGC TGCCATCCAC 
AGCGACGCGC TCTTCCTGTC CAGCCACCTC ATCATTGATT ACTCCCTGCT GGTGGGCCGA 
GACGATTCCA CAGATGAGCT AGTTGTGGGG ATCATAGATT ATATCCGGAC TTTCACTTGG 
GATAAAAAGC TGGAGATGGT GGTCAAATCT ACTGGGATTC TTGGAGGTCA AGGTAAAATG 
CCCACAGTGG TGTCACCAGA GCTGTATCGA ACACGTTTCT GTGAGGCCAT GGATAAATAC 
TTCCTTATGG TCCCTGATCA CTGGAGTGGA CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303000.1
CDS	164..6451
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQSHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_009304725.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304725.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAT GGAAGTCTTG CTCTCCGAAG ATGGAGGTCA GCATATATCC 
ATCAGTGATG CATTCATCAA AGAGTCTTTG TTCAACCGGA GGGTTGAGGA GAAAGCTAAA 
GAGATGCTCT TCACTCCTCT CGGCTGGCAT CACAGCTCTC TGGATCAGCT GCGGGAAGAG 
AATGGGGAAA AGAAAGCGAT GGAGCGGTTG CTGTCAGCAA ATCACAGCCA CATGATGGCG 
CTGCTGCAGC AACTGCTGTA CAGTGAATCA CTGTCTCTGT CGTGGCGTGA CATCATCGTA 
CCTGTGGTGA GGCAGGTGGT GCAGACGGTG CGACCGGATG TGCGCAGCTG TGACGATGAT 
ATGGACATCC GTCAGTTTGT CCACATCAAG AAGATTCCAG GAGGAAAGAA GTTTGACTCT 
GCTGTAGTGA ATGGTTTTGT TTGCACAAAG AATATCGCAC ACAAAAAGAT GAACTCTTAC 
ATCAAAAACC CCAAGATCCT TCTTCTCAAA TGCTCCATTG AGTATCTATA TAGAGAGGAG 
ACCAAGTTCA CCTGCATTGA CCCAATTGTT TTACAGGAGC GTGAGTTTCT TAAGAACTAT 
GTTCAGAGGA TTGTTGATGT CCGACCAAAC TTAGTCCTGG TGGAGAAGAC TGTATCGCGA 
ATTGCTCAGG ACATGCTACT AGAGCACGGC ATTGCCATTG TGATTAATGT CAAACCTCAA 
GTACTGGACC GTGTGAGTCG AATGACTCAG GGAGATTTAG TCATTTCAAT GGATCAGCTT 
CTTACAAAAC CTCGTCTGGG TACCTGCCAC AAGTTTTACT TGCACTCCTT CCAGCTACCA 
AATAGTGAAG TGAAAACCCT GATGTTTTTT GAGGGCTGTC CTCCTCAGCT GGGCTGCACT 
ATTAAGCTCC GCGGAGCCTC AGAGTATGAG CTGGCACGGG TAAAAGAGAT CATCATTTTT 
ATGGTGTGTG TGGCATACCA CTCGCAGCTA GAGATCTCTT TCCTCATGGA TGAGTTCGCT 
ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT TTCCCATGTC TGCTGGAAAG CACCACTTTA 
CGGGAAGAGG ATGAAACTAG TGGGGAACGG GAAAGCCAAG ACAATGATGA GTCTACACTG 
GGAGATGACA ATCTCACACT TGTTCCAGGA GATGACTTTG AGACAGGATT TACAGGGACT 
ATCAAAGCCC ACAGTCGAGA GCCTTCCACC TCTGAATCTT CTCATAAAGA TGGAGAAAGC 
CCCAGAATAA ATAAAAATGC TTCAATTACC TCCTTCTCTG GAGGGGAGGA AGAAAATATA 
AAGACCTCCA CGCCACTTTC CTCCTTCTCT TCCACCTTTC CTCAGCCGGT GACACCACCT 
TTCCTGATAT CAGACGTAAA AGAAATGTCA CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG 
GAGAAGAACA AATATCTGGA GTCGACTCTG GTCCATCAGG ACAGCACAAG CTCTGAGACC 
TCCCTTCCCC CAACTCGGCT CTTTAGAGAC CCCTTACAGG ACGACACAGG CCTGTTTGTG 
ACAGAGCATG TAGCTTCTTC AGATGACCGC CTCAAATCCA TCTCTGCTTT ATTCAAACAG 
GAGCTAAAAG ACATCATCCT GTGCATTTCA CCCTTTATTA CCTTTCGAGA GCCTTTCTTG 
CTTACCCCAG CTGGACTGCG GTGTCCTAGC CGGGATTATT TCCCTGAACA GGTTTACCTT 
TCACCTCTGC TGAACAAGGA TTCAAAAGAA CTGGATGGAC GCCGCAAGAG GCAGCTGCTG 
AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG ACCAATGGTT CCGTTTCACA CCAGAGAGCC 
ATTCAGATCT TGCCCTGTCA CAAACTCACT AGTGCCCGAA TAGCAGAACA GGTAGGCAGC 
AGTCAGCAGC TGGCACGCAT GTTAGCGGAC TACCGTGCCC AAGGAGGACG TATCCGGCAA 
AGAGAGGGAA CACAATTTCG TGAAGCCCCT CCCCCAAAGG CGCCAATAAA GGCAGATAGT 
GAAGAAGATA AAGGTGCAGG AGTGATTGAC ATGACCTGGT CTACTAAGTT GGACTGTTTA 
AATCCAATCA ACCATCAGAG GCTCTGTGTT CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT 
AATGCCCCAA ACCCCTGCGT CAGTCCCTGG ATTGTTACAA TGGAGTTTTA TGGAAAGAAT 
GACTTGACTC TTGGTGTGTT TCTGGAGAGA TACTGTTTTA GACCATCTTA CCAGTGCCCC 
AGCATGTACT GTGAGACCCC CATGGTTCAC CACATCCGGC GCTTTGTGCA CGGTAAAGGC 
TGCGTCCAGA TTGTACTGAA AGAGCTGGAC TCGCCTGTTC CTGGATATCA GCACACAATC 
CTCAACTACT CATGGTGTCG CATCTGCAAA CAGGTGACTC CTGTGGTCCC TTTGTCTAAT 
GACTCTTGGT CCATGTCCTT CGCCAAGTAC CTTGAGCTTC GTTTCTATGG TCATCAGTAC 
ACCCGTCGGG CTAACGCTGA GCCTTGTGGC CACTCTATCC ATAAAGATTA TCACCAGTAC 
TTCTCTTACA ACCAGATGGT GGCATCCTTC AGCTACATTC CAGTGAGGCT GTTAGAGATC 
TGTCTGCCTC CCCCGAAGAT CATCATCAGG AACCAAGGTC CATCGAAATC CAGCTTGCAG 
CAGGATCTCA AAGACTTCTC CCAGAAGGTG GCTCAGGTGT ACCTGGCTAT AGATGACCGT 
CTTACCTCCT TAAAAACGGA CACATTCAGC AAGACCAGAG AGGAAAAAAT GGAGGACATG 
TTTGCACAGA AAGATATGGA GGAATCAGAG CTTCGGGGCT GGATAGAGAA GTTACAAGTG 
CGTCTGCAGA CGAGTGTGAT TGACTCTTCA CAACAACTAC AGGCTGTTCT GGAGTCAGTG 
GTGGTCAAAA AACAGGGCTT GTGTGAAACC CTGCAGTCCT GGAACAACAG ACTTCAAGAA 
CTGTTCCAGC AAGAGAAAGG CAGAAAACGT CTATCTGTTC CTCCTAGCCC AGGCAGACAC 
AGACAACCTA CAACAGATGA GAGCAAGACT AGTGCTTTGG AGTCTTCTCC TCGTAACCCT 
TCCCCAGTGG TCCCAAATGG AGACAAAGAG GACCGTCATC TCAATACATT TCCATCAAGT 
TCAGGGTCCT CGTCGTTACT ACAGTTACCA TCTCCAGCTG AACAACCTTC AGATGTCATT 
ACAAGCGGAC CATCTTTCCC TGATCAGGAC TCTGTTAGCA ATCCAGAGGA CATGTTTGAT 
GGACACTTGC TTGGTTCCAA TGATAGTCAG GTAAAGGAAA AGTCCACCAT GAAAACAATC 
CTGGCCAACC TGTTACCTGG AAACAGCTAC AATCCCATCC CTTTTCCTTT TGACCCAGAC 
AAGCACTATC TGATGTATGA GCATGAGAGA GTTCCTATAG CCGTGTGTGA GAGAGAGCCC 
AGCTCCATAA TTGCCTTTGC ACTCAGCTGC AAAGAGTATA AAACTGCCCT TGAAGAGCTC 
ACAAAGACAA CAGCAAAGAC TGGAGGTGAT GACACTTCTC AGACCATCAG TGCTGGAGAG 
AGCAGAGTGA AGAACAGTCC AGCCAAACCC AGTGACAACA GCATGTCACA GCTGAGCCGC 
AGCAGCGTCG ACGCTGATCC ACTCAAGGAG CCTGAAAGTG CAGACAAACA GAAGAAGCAG 
ACAGGGAACC CACATATTGA GCTGCAGTTC TCCGATGCAA ATGCTAAGTT CTACTGCCGG 
ATCTACTATG CTGAAGAGTT CCATAAAATG CGGGAGGAGA TCATGGAGAG CACAGAGGAT 
GATTTTGTGC GATCTCTTTC CCACTGTGTC AACTGGCAGG CGCGTGGTGG GAAATCTGGT 
GCAGTTTTCT ATGCCACTGA AGATGATCGG TTTATTCTGA AGCAGATGCC CAGATTAGAG 
GTCCAGTCCT TTTTAGACTT TGCACCCCAC TACTTTACGT ACATCACAGG AGCGGTTCAC 
CTAAAGCGAC CCACAGCACT TGCTAAGATT CTGGGAGTGT ACCGCATCGG CTACAAGAAC 
TCCCAGAACA ACACTGAGAA GAAGCTTGAC CTGCTTGTGA TGGAAAACCT TTTCTATGGA 
CGAAAGATGG CACAGGTGTT TGACCTGAAA GGATCGCTGA GAAACAGGAA CGTGAAGACG 
GACCAGGGAA AGGAGAGCTG TGAGGTGGTG CTCCTAGATG AGAACCTGCT CAAACTGGTG 
CATGACAATC CCCTTTATAT TCGCTCGCAC TGCAAGGCCA TCCTGCGTGC TGCCATCCAC 
AGCGACGCGC TCTTCCTGTC CAGCCACCTC ATCATTGATT ACTCCCTGCT GGTGGGCCGA 
GACGATTCCA CAGATGAGCT AGTTGTGGGG ATCATAGATT ATATCCGGAC TTTCACTTGG 
GATAAAAAGC TGGAGATGGT GGTCAAATCT ACTGGGATTC TTGGAGGTCA AGGTAAAATG 
CCCACAGTGG TGTCACCAGA GCTGTATCGA ACACGTTTCT GTGAGGCCAT GGATAAATAC 
TTCCTTATGG TCCCTGATCA CTGGAGTGGA CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( NM_001127305.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001120777.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), mRNA.

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CloneID	ODa15216
Clone ID Related Accession (Same CDS sequence)	XM_005167666.3 , NM_001127305.1 , XM_005167666.4
Accession Version	NM_001127305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU203547.1. On Jul 31, 2008 this sequence version replaced XM_684715.3. ##Evidence-Data-START## Transcript exon combination :: EU203547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAATGGAAG TCTTGCTCTC CGAAGATGGA 
GGTCAGCATA TATCCATCAG TGATGCATTC ATCAAAGAGT CTTTGTTCAA CCGGAGGGTT 
GAGGAGAAAG CTAAAGAGAT GCTCTTCACT CCTCTCGGCT GGCATCACAG CTCTCTGGAT 
CAGCTGCGGG AAGAGAATGG GGAAAAGAAA GCGATGGAGC GGTTGCTGTC AGCAAATCAC 
AGCCACATGA TGGCGCTGCT GCAGCAACTG CTGTACAGTG AATCACTGTC TCTGTCGTGG 
CGTGACATCA TCGTACCTGT GGTGAGGCAG GTGGTGCAGA CGGTGCGACC GGATGTGCGC 
AGCTGTGACG ATGATATGGA CATCCGTCAG TTTGTCCACA TCAAGAAGAT TCCAGGAGGA 
AAGAAGTTTG ACTCTGCTGT AGTGAATGGT TTTGTTTGCA CAAAGAATAT CGCACACAAA 
AAGATGAACT CTTACATCAA AAACCCCAAG ATCCTTCTTC TCAAATGCTC CATTGAGTAT 
CTATATAGAG AGGAGACCAA GTTCACCTGC ATTGACCCAA TTGTTTTACA GGAGCGTGAG 
TTTCTTAAGA ACTATGTTCA GAGGATTGTT GATGTCCGAC CAAACTTAGT CCTGGTGGAG 
AAGACTGTAT CGCGAATTGC TCAGGACATG CTACTAGAGC ACGGCATTGC CATTGTGATT 
AATGTCAAAC CTCAAGTACT GGACCGTGTG AGTCGAATGA CTCAGGGAGA TTTAGTCATT 
TCAATGGATC AGCTTCTTAC AAAACCTCGT CTGGGTACCT GCCACAAGTT TTACTTGCAC 
TCCTTCCAGC TACCAAATAG TGAAGTGAAA ACCCTGATGT TTTTTGAGGG CTGTCCTCCT 
CAGCTGGGCT GCACTATTAA GCTCCGCGGA GCCTCAGAGT ATGAGCTGGC ACGGGTAAAA 
GAGATCATCA TTTTTATGGT GTGTGTGGCA TACCACTCGC AGCTAGAGAT CTCTTTCCTC 
ATGGATGAGT TCGCTATGCC TCCCAGTCTG GCTGAGAGTT CTTCTTTCCC ATGTCTGCTG 
GAAAGCACCA CTTTACGGGA AGAGGATGAA ACTAGTGGGG AACGGGAAAG CCAAGACAAT 
GATGAGTCTA CACTGGGAGA TGACAATCTC ACACTTGTTC CAGGAGATGA CTTTGAGACA 
GGATTTACAG GGACTATCAA AGCCCACAGT CGAGAGCCTT CCACCTCTGA ATCTTCTCAT 
AAAGATGGAG AAAGCCCCAG AATAAATAAA AATGCTTCAA TTACCTCCTT CTCTGGAGGG 
GAGGAAGAAA ATATAAAGAC CTCCACGCCA CTTTCCTCCT TCTCTTCCAC CTTTCCTCAG 
CCGGTGACAC CACCTTTCCT GATATCAGAC GTAAAAGAAA TGTCACAGGA AGTGGTCAAG 
GGGTCAGGTG AAGGGGAGAA GAACAAATAT CTGGAGTCGA CTCTGGTCCA TCAGGACAGC 
ACAAGCTCTG AGACCTCCCT TCCCCCAACT CGGCTCTTTA GAGACCCCTT ACAGGACGAC 
ACAGGCCTGT TTGTGACAGA GCATGTAGCT TCTTCAGATG ACCGCCTCAA ATCCATCTCT 
GCTTTATTCA AACAGGAGCT AAAAGACATC ATCCTGTGCA TTTCACCCTT TATTACCTTT 
CGAGAGCCTT TCTTGCTTAC CCCAGCTGGA CTGCGGTGTC CTAGCCGGGA TTATTTCCCT 
GAACAGGTTT ACCTTTCACC TCTGCTGAAC AAGGATTCAA AAGAACTGGA TGGACGCCGC 
AAGAGGCAGC TGCTGAAAGA GTCCAGCCCA GGTTCTGGTA GCCTGACCAA TGGTTCCGTT 
TCACACCAGA GAGCCATTCA GATCTTGCCC TGTCACAAAC TCACTAGTGC CCGAATAGCA 
GAACAGGTAG GCAGCAGTCA GCAGCTGGCA CGCATGTTAG CGGACTACCG TGCCCAAGGA 
GGACGTATCC GGCAAAGAGA GGGAACACAA TTTCGTGAAG CCCCTCCCCC AAAGGCGCCA 
ATAAAGGCAG ATAGTGAAGA AGATAAAGGT GCAGGAGTGA TTGACATGAC CTGGTCTACT 
AAGTTGGACT GTTTAAATCC AATCAACCAT CAGAGGCTCT GTGTTCTGTT CAGTAGCTCT 
TCAGCACAGT CTAATAATGC CCCAAACCCC TGCGTCAGTC CCTGGATTGT TACAATGGAG 
TTTTATGGAA AGAATGACTT GACTCTTGGT GTGTTTCTGG AGAGATACTG TTTTAGACCA 
TCTTACCAGT GCCCCAGCAT GTACTGTGAG ACCCCCATGG TTCACCACAT CCGGCGCTTT 
GTGCACGGTA AAGGCTGCGT CCAGATTGTA CTGAAAGAGC TGGACTCGCC TGTTCCTGGA 
TATCAGCACA CAATCCTCAA CTACTCATGG TGTCGCATCT GCAAACAGGT GACTCCTGTG 
GTCCCTTTGT CTAATGACTC TTGGTCCATG TCCTTCGCCA AGTACCTTGA GCTTCGTTTC 
TATGGTCATC AGTACACCCG TCGGGCTAAC GCTGAGCCTT GTGGCCACTC TATCCATAAA 
GATTATCACC AGTACTTCTC TTACAACCAG ATGGTGGCAT CCTTCAGCTA CATTCCAGTG 
AGGCTGTTAG AGATCTGTCT GCCTCCCCCG AAGATCATCA TCAGGAACCA AGGTCCATCG 
AAATCCAGCT TGCAGCAGGA TCTCAAAGAC TTCTCCCAGA AGGTGGCTCA GGTGTACCTG 
GCTATAGATG ACCGTCTTAC CTCCTTAAAA ACGGACACAT TCAGCAAGAC CAGAGAGGAA 
AAAATGGAGG ACATGTTTGC ACAGAAAGAT ATGGAGGAAT CAGAGCTTCG GGGCTGGATA 
GAGAAGTTAC AAGTGCGTCT GCAGACGAGT GTGATTGACT CTTCACAACA ACTACAGGCT 
GTTCTGGAGT CAGTGGTGGT CAAAAAACAG GGCTTGTGTG AAACCCTGCA GTCCTGGAAC 
AACAGACTTC AAGAACTGTT CCAGCAAGAG AAAGGCAGAA AACGTCTATC TGTTCCTCCT 
AGCCCAGGCA GACACAGACA ACCTACAACA GATGAGAGCA AGACTAGTGC TTTGGAGTCT 
TCTCCTCGTA ACCCTTCCCC AGTGGTCCCA AATGGAGACA AAGAGGACCG TCATCTCAAT 
ACATTTCCAT CAAGTTCAGG GTCCTCGTCG TTACTACAGT TACCATCTCC AGCTGAACAA 
CCTTCAGATG TCATTACAAG CGGACCATCT TTCCCTGATC AGGACTCTGT TAGCAATCCA 
GAGGACATGT TTGATGGACA CTTGCTTGGT TCCAATGATA GTCAGGTAAA GGAAAAGTCC 
ACCATGAAAA CAATCCTGGC CAACCTGTTA CCTGGAAACA GCTACAATCC CATCCCTTTT 
CCTTTTGACC CAGACAAGCA CTATCTGATG TATGAGCATG AGAGAGTTCC TATAGCCGTG 
TGTGAGAGAG AGCCCAGCTC CATAATTGCC TTTGCACTCA GCTGCAAAGA GTATAAAACT 
GCCCTTGAAG AGCTCACAAA GACAACAGCA AAGACTGGAG GTGATGACAC TTCTCAGACC 
ATCAGTGCTG GAGAGAGCAG AGTGAAGAAC AGTCCAGCCA AACCCAGTGA CAACAGCATG 
TCACAGCTGA GCCGCAGCAG CGTCGACGCT GATCCACTCA AGGAGCCTGA AAGTGCAGAC 
AAACAGAAGA AGCAGACAGG GAACCCACAT ATTGAGCTGC AGTTCTCCGA TGCAAATGCT 
AAGTTCTACT GCCGGATCTA CTATGCTGAA GAGTTCCATA AAATGCGGGA GGAGATCATG 
GAGAGCACAG AGGATGATTT TGTGCGATCT CTTTCCCACT GTGTCAACTG GCAGGCGCGT 
GGTGGGAAAT CTGGTGCAGT TTTCTATGCC ACTGAAGATG ATCGGTTTAT TCTGAAGCAG 
ATGCCCAGAT TAGAGGTCCA GTCCTTTTTA GACTTTGCAC CCCACTACTT TACGTACATC 
ACAGGAGCGG TTCACCTAAA GCGACCCACA GCACTTGCTA AGATTCTGGG AGTGTACCGC 
ATCGGCTACA AGAACTCCCA GAACAACACT GAGAAGAAGC TTGACCTGCT TGTGATGGAA 
AACCTTTTCT ATGGACGAAA GATGGCACAG GTGTTTGACC TGAAAGGATC GCTGAGAAAC 
AGGAACGTGA AGACGGACCA GGGAAAGGAG AGCTGTGAGG TGGTGCTCCT AGATGAGAAC 
CTGCTCAAAC TGGTGCATGA CAATCCCCTT TATATTCGCT CGCACTGCAA GGCCATCCTG 
CGTGCTGCCA TCCACAGCGA CGCGCTCTTC CTGTCCAGCC ACCTCATCAT TGATTACTCC 
CTGCTGGTGG GCCGAGACGA TTCCACAGAT GAGCTAGTTG TGGGGATCAT AGATTATATC 
CGGACTTTCA CTTGGGATAA AAAGCTGGAG ATGGTGGTCA AATCTACTGG GATTCTTGGA 
GGTCAAGGTA AAATGCCCAC AGTGGTGTCA CCAGAGCTGT ATCGAACACG TTTCTGTGAG 
GCCATGGATA AATACTTCCT TATGGTCCCT GATCACTGGA GTGGACTTGG GCTCAACTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120777.1
CDS	1..6303
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	NM_001127305.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127305.1

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAATGGAAG TCTTGCTCTC CGAAGATGGA 
GGTCAGCATA TATCCATCAG TGATGCATTC ATCAAAGAGT CTTTGTTCAA CCGGAGGGTT 
GAGGAGAAAG CTAAAGAGAT GCTCTTCACT CCTCTCGGCT GGCATCACAG CTCTCTGGAT 
CAGCTGCGGG AAGAGAATGG GGAAAAGAAA GCGATGGAGC GGTTGCTGTC AGCAAATCAC 
AGCCACATGA TGGCGCTGCT GCAGCAACTG CTGTACAGTG AATCACTGTC TCTGTCGTGG 
CGTGACATCA TCGTACCTGT GGTGAGGCAG GTGGTGCAGA CGGTGCGACC GGATGTGCGC 
AGCTGTGACG ATGATATGGA CATCCGTCAG TTTGTCCACA TCAAGAAGAT TCCAGGAGGA 
AAGAAGTTTG ACTCTGCTGT AGTGAATGGT TTTGTTTGCA CAAAGAATAT CGCACACAAA 
AAGATGAACT CTTACATCAA AAACCCCAAG ATCCTTCTTC TCAAATGCTC CATTGAGTAT 
CTATATAGAG AGGAGACCAA GTTCACCTGC ATTGACCCAA TTGTTTTACA GGAGCGTGAG 
TTTCTTAAGA ACTATGTTCA GAGGATTGTT GATGTCCGAC CAAACTTAGT CCTGGTGGAG 
AAGACTGTAT CGCGAATTGC TCAGGACATG CTACTAGAGC ACGGCATTGC CATTGTGATT 
AATGTCAAAC CTCAAGTACT GGACCGTGTG AGTCGAATGA CTCAGGGAGA TTTAGTCATT 
TCAATGGATC AGCTTCTTAC AAAACCTCGT CTGGGTACCT GCCACAAGTT TTACTTGCAC 
TCCTTCCAGC TACCAAATAG TGAAGTGAAA ACCCTGATGT TTTTTGAGGG CTGTCCTCCT 
CAGCTGGGCT GCACTATTAA GCTCCGCGGA GCCTCAGAGT ATGAGCTGGC ACGGGTAAAA 
GAGATCATCA TTTTTATGGT GTGTGTGGCA TACCACTCGC AGCTAGAGAT CTCTTTCCTC 
ATGGATGAGT TCGCTATGCC TCCCAGTCTG GCTGAGAGTT CTTCTTTCCC ATGTCTGCTG 
GAAAGCACCA CTTTACGGGA AGAGGATGAA ACTAGTGGGG AACGGGAAAG CCAAGACAAT 
GATGAGTCTA CACTGGGAGA TGACAATCTC ACACTTGTTC CAGGAGATGA CTTTGAGACA 
GGATTTACAG GGACTATCAA AGCCCACAGT CGAGAGCCTT CCACCTCTGA ATCTTCTCAT 
AAAGATGGAG AAAGCCCCAG AATAAATAAA AATGCTTCAA TTACCTCCTT CTCTGGAGGG 
GAGGAAGAAA ATATAAAGAC CTCCACGCCA CTTTCCTCCT TCTCTTCCAC CTTTCCTCAG 
CCGGTGACAC CACCTTTCCT GATATCAGAC GTAAAAGAAA TGTCACAGGA AGTGGTCAAG 
GGGTCAGGTG AAGGGGAGAA GAACAAATAT CTGGAGTCGA CTCTGGTCCA TCAGGACAGC 
ACAAGCTCTG AGACCTCCCT TCCCCCAACT CGGCTCTTTA GAGACCCCTT ACAGGACGAC 
ACAGGCCTGT TTGTGACAGA GCATGTAGCT TCTTCAGATG ACCGCCTCAA ATCCATCTCT 
GCTTTATTCA AACAGGAGCT AAAAGACATC ATCCTGTGCA TTTCACCCTT TATTACCTTT 
CGAGAGCCTT TCTTGCTTAC CCCAGCTGGA CTGCGGTGTC CTAGCCGGGA TTATTTCCCT 
GAACAGGTTT ACCTTTCACC TCTGCTGAAC AAGGATTCAA AAGAACTGGA TGGACGCCGC 
AAGAGGCAGC TGCTGAAAGA GTCCAGCCCA GGTTCTGGTA GCCTGACCAA TGGTTCCGTT 
TCACACCAGA GAGCCATTCA GATCTTGCCC TGTCACAAAC TCACTAGTGC CCGAATAGCA 
GAACAGGTAG GCAGCAGTCA GCAGCTGGCA CGCATGTTAG CGGACTACCG TGCCCAAGGA 
GGACGTATCC GGCAAAGAGA GGGAACACAA TTTCGTGAAG CCCCTCCCCC AAAGGCGCCA 
ATAAAGGCAG ATAGTGAAGA AGATAAAGGT GCAGGAGTGA TTGACATGAC CTGGTCTACT 
AAGTTGGACT GTTTAAATCC AATCAACCAT CAGAGGCTCT GTGTTCTGTT CAGTAGCTCT 
TCAGCACAGT CTAATAATGC CCCAAACCCC TGCGTCAGTC CCTGGATTGT TACAATGGAG 
TTTTATGGAA AGAATGACTT GACTCTTGGT GTGTTTCTGG AGAGATACTG TTTTAGACCA 
TCTTACCAGT GCCCCAGCAT GTACTGTGAG ACCCCCATGG TTCACCACAT CCGGCGCTTT 
GTGCACGGTA AAGGCTGCGT CCAGATTGTA CTGAAAGAGC TGGACTCGCC TGTTCCTGGA 
TATCAGCACA CAATCCTCAA CTACTCATGG TGTCGCATCT GCAAACAGGT GACTCCTGTG 
GTCCCTTTGT CTAATGACTC TTGGTCCATG TCCTTCGCCA AGTACCTTGA GCTTCGTTTC 
TATGGTCATC AGTACACCCG TCGGGCTAAC GCTGAGCCTT GTGGCCACTC TATCCATAAA 
GATTATCACC AGTACTTCTC TTACAACCAG ATGGTGGCAT CCTTCAGCTA CATTCCAGTG 
AGGCTGTTAG AGATCTGTCT GCCTCCCCCG AAGATCATCA TCAGGAACCA AGGTCCATCG 
AAATCCAGCT TGCAGCAGGA TCTCAAAGAC TTCTCCCAGA AGGTGGCTCA GGTGTACCTG 
GCTATAGATG ACCGTCTTAC CTCCTTAAAA ACGGACACAT TCAGCAAGAC CAGAGAGGAA 
AAAATGGAGG ACATGTTTGC ACAGAAAGAT ATGGAGGAAT CAGAGCTTCG GGGCTGGATA 
GAGAAGTTAC AAGTGCGTCT GCAGACGAGT GTGATTGACT CTTCACAACA ACTACAGGCT 
GTTCTGGAGT CAGTGGTGGT CAAAAAACAG GGCTTGTGTG AAACCCTGCA GTCCTGGAAC 
AACAGACTTC AAGAACTGTT CCAGCAAGAG AAAGGCAGAA AACGTCTATC TGTTCCTCCT 
AGCCCAGGCA GACACAGACA ACCTACAACA GATGAGAGCA AGACTAGTGC TTTGGAGTCT 
TCTCCTCGTA ACCCTTCCCC AGTGGTCCCA AATGGAGACA AAGAGGACCG TCATCTCAAT 
ACATTTCCAT CAAGTTCAGG GTCCTCGTCG TTACTACAGT TACCATCTCC AGCTGAACAA 
CCTTCAGATG TCATTACAAG CGGACCATCT TTCCCTGATC AGGACTCTGT TAGCAATCCA 
GAGGACATGT TTGATGGACA CTTGCTTGGT TCCAATGATA GTCAGGTAAA GGAAAAGTCC 
ACCATGAAAA CAATCCTGGC CAACCTGTTA CCTGGAAACA GCTACAATCC CATCCCTTTT 
CCTTTTGACC CAGACAAGCA CTATCTGATG TATGAGCATG AGAGAGTTCC TATAGCCGTG 
TGTGAGAGAG AGCCCAGCTC CATAATTGCC TTTGCACTCA GCTGCAAAGA GTATAAAACT 
GCCCTTGAAG AGCTCACAAA GACAACAGCA AAGACTGGAG GTGATGACAC TTCTCAGACC 
ATCAGTGCTG GAGAGAGCAG AGTGAAGAAC AGTCCAGCCA AACCCAGTGA CAACAGCATG 
TCACAGCTGA GCCGCAGCAG CGTCGACGCT GATCCACTCA AGGAGCCTGA AAGTGCAGAC 
AAACAGAAGA AGCAGACAGG GAACCCACAT ATTGAGCTGC AGTTCTCCGA TGCAAATGCT 
AAGTTCTACT GCCGGATCTA CTATGCTGAA GAGTTCCATA AAATGCGGGA GGAGATCATG 
GAGAGCACAG AGGATGATTT TGTGCGATCT CTTTCCCACT GTGTCAACTG GCAGGCGCGT 
GGTGGGAAAT CTGGTGCAGT TTTCTATGCC ACTGAAGATG ATCGGTTTAT TCTGAAGCAG 
ATGCCCAGAT TAGAGGTCCA GTCCTTTTTA GACTTTGCAC CCCACTACTT TACGTACATC 
ACAGGAGCGG TTCACCTAAA GCGACCCACA GCACTTGCTA AGATTCTGGG AGTGTACCGC 
ATCGGCTACA AGAACTCCCA GAACAACACT GAGAAGAAGC TTGACCTGCT TGTGATGGAA 
AACCTTTTCT ATGGACGAAA GATGGCACAG GTGTTTGACC TGAAAGGATC GCTGAGAAAC 
AGGAACGTGA AGACGGACCA GGGAAAGGAG AGCTGTGAGG TGGTGCTCCT AGATGAGAAC 
CTGCTCAAAC TGGTGCATGA CAATCCCCTT TATATTCGCT CGCACTGCAA GGCCATCCTG 
CGTGCTGCCA TCCACAGCGA CGCGCTCTTC CTGTCCAGCC ACCTCATCAT TGATTACTCC 
CTGCTGGTGG GCCGAGACGA TTCCACAGAT GAGCTAGTTG TGGGGATCAT AGATTATATC 
CGGACTTTCA CTTGGGATAA AAAGCTGGAG ATGGTGGTCA AATCTACTGG GATTCTTGGA 
GGTCAAGGTA AAATGCCCAC AGTGGTGTCA CCAGAGCTGT ATCGAACACG TTTCTGTGAG 
GCCATGGATA AATACTTCCT TATGGTCCCT GATCACTGGA GTGGACTTGG GCTCAACTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167665.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167722.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.

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CloneID	ODa15215
Clone ID Related Accession (Same CDS sequence)	XM_005167665.3 , XM_005167665.4
Accession Version	XM_005167665.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAATGGA AGTCTTGCTC 
TCCGAAGATG GAGGTCAGCA TATATCCATC AGTGATGCAT TCATCAAAGA GTCTTTGTTC 
AACCGGAGGG TTGAGGAGAA AGCTAAAGAG ATGCTCTTCA CTCCTCTCGG CTGGCATCAC 
AGCTCTCTGG ATCAGCTGCG GGAAGAGAAT GGGGAAAAGA AAGCGATGGA GCGGTTGCTG 
TCAGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAAC TGCTGTACAG TGAATCACTG 
TCTCTGTCGT GGCGTGACAT CATCGTACCT GTGGTGAGGC AGGTGGTGCA GACGGTGCGA 
CCGGATGTGC GCAGCTGTGA CGATGATATG GACATCCGTC AGTTTGTCCA CATCAAGAAG 
ATTCCAGGAG GAAAGAAGTT TGACTCTGCT GTAGTGAATG GTTTTGTTTG CACAAAGAAT 
ATCGCACACA AAAAGATGAA CTCTTACATC AAAAACCCCA AGATCCTTCT TCTCAAATGC 
TCCATTGAGT ATCTATATAG AGAGGAGACC AAGTTCACCT GCATTGACCC AATTGTTTTA 
CAGGAGCGTG AGTTTCTTAA GAACTATGTT CAGAGGATTG TTGATGTCCG ACCAAACTTA 
GTCCTGGTGG AGAAGACTGT ATCGCGAATT GCTCAGGACA TGCTACTAGA GCACGGCATT 
GCCATTGTGA TTAATGTCAA ACCTCAAGTA CTGGACCGTG TGAGTCGAAT GACTCAGGGA 
GATTTAGTCA TTTCAATGGA TCAGCTTCTT ACAAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACTTGC ACTCCTTCCA GCTACCAAAT AGTGAAGTGA AAACCCTGAT GTTTTTTGAG 
GGCTGTCCTC CTCAGCTGGG CTGCACTATT AAGCTCCGCG GAGCCTCAGA GTATGAGCTG 
GCACGGGTAA AAGAGATCAT CATTTTTATG GTGTGTGTGG CATACCACTC GCAGCTAGAG 
ATCTCTTTCC TCATGGATGA GTTCGCTATG CCTCCCAGTC TGGCTGAGAG TTCTTCTTTC 
CCATGTCTGC TGGAAAGCAC CACTTTACGG GAAGAGGATG AAACTAGTGG GGAACGGGAA 
AGCCAAGACA ATGATGAGTC TACACTGGGA GATGACAATC TCACACTTGT TCCAGGAGAT 
GACTTTGAGA CAGGATTTAC AGGGACTATC AAAGCCCACA GTCGAGAGCC TTCCACCTCT 
GAATCTTCTC ATAAAGATGG AGAAAGCCCC AGAATAAATA AAAATGCTTC AATTACCTCC 
TTCTCTGGAG GGGAGGAAGA AAATATAAAG ACCTCCACGC CACTTTCCTC CTTCTCTTCC 
ACCTTTCCTC AGCCGGTGAC ACCACCTTTC CTGATATCAG ACGTAAAAGA AATGTCACAG 
GAAGTGGTCA AGGGGTCAGG TGAAGGGGAG AAGAACAAAT ATCTGGAGTC GACTCTGGTC 
CATCAGGACA GCACAAGCTC TGAGACCTCC CTTCCCCCAA CTCGGCTCTT TAGAGACCCC 
TTACAGGACG ACACAGGCCT GTTTGTGACA GAGCATGTAG CTTCTTCAGA TGACCGCCTC 
AAATCCATCT CTGCTTTATT CAAACAGGAG CTAAAAGACA TCATCCTGTG CATTTCACCC 
TTTATTACCT TTCGAGAGCC TTTCTTGCTT ACCCCAGCTG GACTGCGGTG TCCTAGCCGG 
GATTATTTCC CTGAACAGGT TTACCTTTCA CCTCTGCTGA ACAAGGATTC AAAAGAACTG 
GATGGACGCC GCAAGAGGCA GCTGCTGAAA GAGTCCAGCC CAGGTTCTGG TAGCCTGACC 
AATGGTTCCG TTTCACACCA GAGAGCCATT CAGATCTTGC CCTGTCACAA ACTCACTAGT 
GCCCGAATAG CAGAACAGGT AGGCAGCAGT CAGCAGCTGG CACGCATGTT AGCGGACTAC 
CGTGCCCAAG GAGGACGTAT CCGGCAAAGA GAGGGAACAC AATTTCGTGA AGCCCCTCCC 
CCAAAGGCGC CAATAAAGGC AGATAGTGAA GAAGATAAAG GTGCAGGAGT GATTGACATG 
ACCTGGTCTA CTAAGTTGGA CTGTTTAAAT CCAATCAACC ATCAGAGGCT CTGTGTTCTG 
TTCAGTAGCT CTTCAGCACA GTCTAATAAT GCCCCAAACC CCTGCGTCAG TCCCTGGATT 
GTTACAATGG AGTTTTATGG AAAGAATGAC TTGACTCTTG GTGTGTTTCT GGAGAGATAC 
TGTTTTAGAC CATCTTACCA GTGCCCCAGC ATGTACTGTG AGACCCCCAT GGTTCACCAC 
ATCCGGCGCT TTGTGCACGG TAAAGGCTGC GTCCAGATTG TACTGAAAGA GCTGGACTCG 
CCTGTTCCTG GATATCAGCA CACAATCCTC AACTACTCAT GGTGTCGCAT CTGCAAACAG 
GTGACTCCTG TGGTCCCTTT GTCTAATGAC TCTTGGTCCA TGTCCTTCGC CAAGTACCTT 
GAGCTTCGTT TCTATGGTCA TCAGTACACC CGTCGGGCTA ACGCTGAGCC TTGTGGCCAC 
TCTATCCATA AAGATTATCA CCAGTACTTC TCTTACAACC AGATGGTGGC ATCCTTCAGC 
TACATTCCAG TGAGGCTGTT AGAGATCTGT CTGCCTCCCC CGAAGATCAT CATCAGGAAC 
CAAGGTCCAT CGAAATCCAG CTTGCAGCAG GATCTCAAAG ACTTCTCCCA GAAGGTGGCT 
CAGGTGTACC TGGCTATAGA TGACCGTCTT ACCTCCTTAA AAACGGACAC ATTCAGCAAG 
ACCAGAGAGG AAAAAATGGA GGACATGTTT GCACAGAAAG ATATGGAGGA ATCAGAGCTT 
CGGGGCTGGA TAGAGAAGTT ACAAGTGCGT CTGCAGACGA GTGTGATTGA CTCTTCACAA 
CAACTACAGG CTGTTCTGGA GTCAGTGGTG GTCAAAAAAC AGGGCTTGTG TGAAACCCTG 
CAGTCCTGGA ACAACAGACT TCAAGAACTG TTCCAGCAAG AGAAAGGCAG AAAACGTCTA 
TCTGTTCCTC CTAGCCCAGG CAGACACAGA CAACCTACAA CAGATGAGAG CAAGACTAGT 
GCTTTGGAGT CTTCTCCTCG TAACCCTTCC CCAGTGGTCC CAAATGGAGA CAAAGAGGAC 
CGTCATCTCA ATACATTTCC ATCAAGTTCA GGGTCCTCGT CGTTACTACA GTTACCATCT 
CCAGCTGAAC AACCTTCAGA TGTCATTACA AGCGGACCAT CTTTCCCTGA TCAGGACTCT 
GTTAGCAATC CAGAGGACAT GTTTGATGGA CACTTGCTTG GTTCCAATGA TAGTCAGGTA 
AAGGAAAAGT CCACCATGAA AACAATCCTG GCCAACCTGT TACCTGGAAA CAGCTACAAT 
CCCATCCCTT TTCCTTTTGA CCCAGACAAG CACTATCTGA TGTATGAGCA TGAGAGAGTT 
CCTATAGCCG TGTGTGAGAG AGAGCCCAGC TCCATAATTG CCTTTGCACT CAGCTGCAAA 
GAGTATAAAA CTGCCCTTGA AGAGCTCACA AAGACAACAG CAAAGACTGG AGGTGATGAC 
ACTTCTCAGA CCATCAGTGC TGGAGAGAGC AGAGTGAAGA ACAGTCCAGC CAAACCCAGT 
GACAACAGCA TGTCACAGCT GAGCCGCAGC AGCGTCGACG CTGATCCACT CAAGGAGCCT 
GAAAGTGCAG ACAAACAGAA GAAGCAGACA GGGAACCCAC ATATTGAGCT GCAGTTCTCC 
GATGCAAATG CTAAGTTCTA CTGCCGGATC TACTATGCTG AAGAGTTCCA TAAAATGCGG 
GAGGAGATCA TGGAGAGCAC AGAGGATGAT TTTGTGCGAT CTCTTTCCCA CTGTGTCAAC 
TGGCAGGCGC GTGGTGGGAA ATCTGGTGCA GTTTTCTATG CCACTGAAGA TGATCGGTTT 
ATTCTGAAGC AGATGCCCAG ATTAGAGGTC CAGTCCTTTT TAGACTTTGC ACCCCACTAC 
TTTACGTACA TCACAGGAGC GGTTCACCTA AAGCGACCCA CAGCACTTGC TAAGATTCTG 
GGAGTGTACC GCATCGGCTA CAAGAACTCC CAGAACAACA CTGAGAAGAA GCTTGACCTG 
CTTGTGATGG AAAACCTTTT CTATGGACGA AAGATGGCAC AGGTGTTTGA CCTGAAAGGA 
TCGCTGAGAA ACAGGAACGT GAAGACGGAC CAGGGAAAGG AGAGCTGTGA GGTGGTGCTC 
CTAGATGAGA ACCTGCTCAA ACTGGTGCAT GACAATCCCC TTTATATTCG CTCGCACTGC 
AAGGCCATCC TGCGTGCTGC CATCCACAGC GACGCGCTCT TCCTGTCCAG CCACCTCATC 
ATTGATTACT CCCTGCTGGT GGGCCGAGAC GATTCCACAG ATGAGCTAGT TGTGGGGATC 
ATAGATTATA TCCGGACTTT CACTTGGGAT AAAAAGCTGG AGATGGTGGT CAAATCTACT 
GGGATTCTTG GAGGTCAAGG TAAAATGCCC ACAGTGGTGT CACCAGAGCT GTATCGAACA 
CGTTTCTGTG AGGCCATGGA TAAATACTTC CTTATGGTCC CTGATCACTG GAGTGGACTT 
GGGCTCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167722.1
CDS	156..6470
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167665.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167665.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAATGGA AGTCTTGCTC 
TCCGAAGATG GAGGTCAGCA TATATCCATC AGTGATGCAT TCATCAAAGA GTCTTTGTTC 
AACCGGAGGG TTGAGGAGAA AGCTAAAGAG ATGCTCTTCA CTCCTCTCGG CTGGCATCAC 
AGCTCTCTGG ATCAGCTGCG GGAAGAGAAT GGGGAAAAGA AAGCGATGGA GCGGTTGCTG 
TCAGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAAC TGCTGTACAG TGAATCACTG 
TCTCTGTCGT GGCGTGACAT CATCGTACCT GTGGTGAGGC AGGTGGTGCA GACGGTGCGA 
CCGGATGTGC GCAGCTGTGA CGATGATATG GACATCCGTC AGTTTGTCCA CATCAAGAAG 
ATTCCAGGAG GAAAGAAGTT TGACTCTGCT GTAGTGAATG GTTTTGTTTG CACAAAGAAT 
ATCGCACACA AAAAGATGAA CTCTTACATC AAAAACCCCA AGATCCTTCT TCTCAAATGC 
TCCATTGAGT ATCTATATAG AGAGGAGACC AAGTTCACCT GCATTGACCC AATTGTTTTA 
CAGGAGCGTG AGTTTCTTAA GAACTATGTT CAGAGGATTG TTGATGTCCG ACCAAACTTA 
GTCCTGGTGG AGAAGACTGT ATCGCGAATT GCTCAGGACA TGCTACTAGA GCACGGCATT 
GCCATTGTGA TTAATGTCAA ACCTCAAGTA CTGGACCGTG TGAGTCGAAT GACTCAGGGA 
GATTTAGTCA TTTCAATGGA TCAGCTTCTT ACAAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACTTGC ACTCCTTCCA GCTACCAAAT AGTGAAGTGA AAACCCTGAT GTTTTTTGAG 
GGCTGTCCTC CTCAGCTGGG CTGCACTATT AAGCTCCGCG GAGCCTCAGA GTATGAGCTG 
GCACGGGTAA AAGAGATCAT CATTTTTATG GTGTGTGTGG CATACCACTC GCAGCTAGAG 
ATCTCTTTCC TCATGGATGA GTTCGCTATG CCTCCCAGTC TGGCTGAGAG TTCTTCTTTC 
CCATGTCTGC TGGAAAGCAC CACTTTACGG GAAGAGGATG AAACTAGTGG GGAACGGGAA 
AGCCAAGACA ATGATGAGTC TACACTGGGA GATGACAATC TCACACTTGT TCCAGGAGAT 
GACTTTGAGA CAGGATTTAC AGGGACTATC AAAGCCCACA GTCGAGAGCC TTCCACCTCT 
GAATCTTCTC ATAAAGATGG AGAAAGCCCC AGAATAAATA AAAATGCTTC AATTACCTCC 
TTCTCTGGAG GGGAGGAAGA AAATATAAAG ACCTCCACGC CACTTTCCTC CTTCTCTTCC 
ACCTTTCCTC AGCCGGTGAC ACCACCTTTC CTGATATCAG ACGTAAAAGA AATGTCACAG 
GAAGTGGTCA AGGGGTCAGG TGAAGGGGAG AAGAACAAAT ATCTGGAGTC GACTCTGGTC 
CATCAGGACA GCACAAGCTC TGAGACCTCC CTTCCCCCAA CTCGGCTCTT TAGAGACCCC 
TTACAGGACG ACACAGGCCT GTTTGTGACA GAGCATGTAG CTTCTTCAGA TGACCGCCTC 
AAATCCATCT CTGCTTTATT CAAACAGGAG CTAAAAGACA TCATCCTGTG CATTTCACCC 
TTTATTACCT TTCGAGAGCC TTTCTTGCTT ACCCCAGCTG GACTGCGGTG TCCTAGCCGG 
GATTATTTCC CTGAACAGGT TTACCTTTCA CCTCTGCTGA ACAAGGATTC AAAAGAACTG 
GATGGACGCC GCAAGAGGCA GCTGCTGAAA GAGTCCAGCC CAGGTTCTGG TAGCCTGACC 
AATGGTTCCG TTTCACACCA GAGAGCCATT CAGATCTTGC CCTGTCACAA ACTCACTAGT 
GCCCGAATAG CAGAACAGGT AGGCAGCAGT CAGCAGCTGG CACGCATGTT AGCGGACTAC 
CGTGCCCAAG GAGGACGTAT CCGGCAAAGA GAGGGAACAC AATTTCGTGA AGCCCCTCCC 
CCAAAGGCGC CAATAAAGGC AGATAGTGAA GAAGATAAAG GTGCAGGAGT GATTGACATG 
ACCTGGTCTA CTAAGTTGGA CTGTTTAAAT CCAATCAACC ATCAGAGGCT CTGTGTTCTG 
TTCAGTAGCT CTTCAGCACA GTCTAATAAT GCCCCAAACC CCTGCGTCAG TCCCTGGATT 
GTTACAATGG AGTTTTATGG AAAGAATGAC TTGACTCTTG GTGTGTTTCT GGAGAGATAC 
TGTTTTAGAC CATCTTACCA GTGCCCCAGC ATGTACTGTG AGACCCCCAT GGTTCACCAC 
ATCCGGCGCT TTGTGCACGG TAAAGGCTGC GTCCAGATTG TACTGAAAGA GCTGGACTCG 
CCTGTTCCTG GATATCAGCA CACAATCCTC AACTACTCAT GGTGTCGCAT CTGCAAACAG 
GTGACTCCTG TGGTCCCTTT GTCTAATGAC TCTTGGTCCA TGTCCTTCGC CAAGTACCTT 
GAGCTTCGTT TCTATGGTCA TCAGTACACC CGTCGGGCTA ACGCTGAGCC TTGTGGCCAC 
TCTATCCATA AAGATTATCA CCAGTACTTC TCTTACAACC AGATGGTGGC ATCCTTCAGC 
TACATTCCAG TGAGGCTGTT AGAGATCTGT CTGCCTCCCC CGAAGATCAT CATCAGGAAC 
CAAGGTCCAT CGAAATCCAG CTTGCAGCAG GATCTCAAAG ACTTCTCCCA GAAGGTGGCT 
CAGGTGTACC TGGCTATAGA TGACCGTCTT ACCTCCTTAA AAACGGACAC ATTCAGCAAG 
ACCAGAGAGG AAAAAATGGA GGACATGTTT GCACAGAAAG ATATGGAGGA ATCAGAGCTT 
CGGGGCTGGA TAGAGAAGTT ACAAGTGCGT CTGCAGACGA GTGTGATTGA CTCTTCACAA 
CAACTACAGG CTGTTCTGGA GTCAGTGGTG GTCAAAAAAC AGGGCTTGTG TGAAACCCTG 
CAGTCCTGGA ACAACAGACT TCAAGAACTG TTCCAGCAAG AGAAAGGCAG AAAACGTCTA 
TCTGTTCCTC CTAGCCCAGG CAGACACAGA CAACCTACAA CAGATGAGAG CAAGACTAGT 
GCTTTGGAGT CTTCTCCTCG TAACCCTTCC CCAGTGGTCC CAAATGGAGA CAAAGAGGAC 
CGTCATCTCA ATACATTTCC ATCAAGTTCA GGGTCCTCGT CGTTACTACA GTTACCATCT 
CCAGCTGAAC AACCTTCAGA TGTCATTACA AGCGGACCAT CTTTCCCTGA TCAGGACTCT 
GTTAGCAATC CAGAGGACAT GTTTGATGGA CACTTGCTTG GTTCCAATGA TAGTCAGGTA 
AAGGAAAAGT CCACCATGAA AACAATCCTG GCCAACCTGT TACCTGGAAA CAGCTACAAT 
CCCATCCCTT TTCCTTTTGA CCCAGACAAG CACTATCTGA TGTATGAGCA TGAGAGAGTT 
CCTATAGCCG TGTGTGAGAG AGAGCCCAGC TCCATAATTG CCTTTGCACT CAGCTGCAAA 
GAGTATAAAA CTGCCCTTGA AGAGCTCACA AAGACAACAG CAAAGACTGG AGGTGATGAC 
ACTTCTCAGA CCATCAGTGC TGGAGAGAGC AGAGTGAAGA ACAGTCCAGC CAAACCCAGT 
GACAACAGCA TGTCACAGCT GAGCCGCAGC AGCGTCGACG CTGATCCACT CAAGGAGCCT 
GAAAGTGCAG ACAAACAGAA GAAGCAGACA GGGAACCCAC ATATTGAGCT GCAGTTCTCC 
GATGCAAATG CTAAGTTCTA CTGCCGGATC TACTATGCTG AAGAGTTCCA TAAAATGCGG 
GAGGAGATCA TGGAGAGCAC AGAGGATGAT TTTGTGCGAT CTCTTTCCCA CTGTGTCAAC 
TGGCAGGCGC GTGGTGGGAA ATCTGGTGCA GTTTTCTATG CCACTGAAGA TGATCGGTTT 
ATTCTGAAGC AGATGCCCAG ATTAGAGGTC CAGTCCTTTT TAGACTTTGC ACCCCACTAC 
TTTACGTACA TCACAGGAGC GGTTCACCTA AAGCGACCCA CAGCACTTGC TAAGATTCTG 
GGAGTGTACC GCATCGGCTA CAAGAACTCC CAGAACAACA CTGAGAAGAA GCTTGACCTG 
CTTGTGATGG AAAACCTTTT CTATGGACGA AAGATGGCAC AGGTGTTTGA CCTGAAAGGA 
TCGCTGAGAA ACAGGAACGT GAAGACGGAC CAGGGAAAGG AGAGCTGTGA GGTGGTGCTC 
CTAGATGAGA ACCTGCTCAA ACTGGTGCAT GACAATCCCC TTTATATTCG CTCGCACTGC 
AAGGCCATCC TGCGTGCTGC CATCCACAGC GACGCGCTCT TCCTGTCCAG CCACCTCATC 
ATTGATTACT CCCTGCTGGT GGGCCGAGAC GATTCCACAG ATGAGCTAGT TGTGGGGATC 
ATAGATTATA TCCGGACTTT CACTTGGGAT AAAAAGCTGG AGATGGTGGT CAAATCTACT 
GGGATTCTTG GAGGTCAAGG TAAAATGCCC ACAGTGGTGT CACCAGAGCT GTATCGAACA 
CGTTTCTGTG AGGCCATGGA TAAATACTTC CTTATGGTCC CTGATCACTG GAGTGGACTT 
GGGCTCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167665.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167722.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.

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CloneID	ODa15215
Clone ID Related Accession (Same CDS sequence)	XM_005167665.3 , XM_005167665.4
Accession Version	XM_005167665.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167665.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAATGGA AGTCTTGCTC 
TCCGAAGATG GAGGTCAGCA TATATCCATC AGTGATGCAT TCATCAAAGA GTCTTTGTTC 
AACCGGAGGG TTGAGGAGAA AGCTAAAGAG ATGCTCTTCA CTCCTCTCGG CTGGCATCAC 
AGCTCTCTGG ATCAGCTGCG GGAAGAGAAT GGGGAAAAGA AAGCGATGGA GCGGTTGCTG 
TCAGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAAC TGCTGTACAG TGAATCACTG 
TCTCTGTCGT GGCGTGACAT CATCGTACCT GTGGTGAGGC AGGTGGTGCA GACGGTGCGA 
CCGGATGTGC GCAGCTGTGA CGATGATATG GACATCCGTC AGTTTGTCCA CATCAAGAAG 
ATTCCAGGAG GAAAGAAGTT TGACTCTGCT GTAGTGAATG GTTTTGTTTG CACAAAGAAT 
ATCGCACACA AAAAGATGAA CTCTTACATC AAAAACCCCA AGATCCTTCT TCTCAAATGC 
TCCATTGAGT ATCTATATAG AGAGGAGACC AAGTTCACCT GCATTGACCC AATTGTTTTA 
CAGGAGCGTG AGTTTCTTAA GAACTATGTT CAGAGGATTG TTGATGTCCG ACCAAACTTA 
GTCCTGGTGG AGAAGACTGT ATCGCGAATT GCTCAGGACA TGCTACTAGA GCACGGCATT 
GCCATTGTGA TTAATGTCAA ACCTCAAGTA CTGGACCGTG TGAGTCGAAT GACTCAGGGA 
GATTTAGTCA TTTCAATGGA TCAGCTTCTT ACAAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACTTGC ACTCCTTCCA GCTACCAAAT AGTGAAGTGA AAACCCTGAT GTTTTTTGAG 
GGCTGTCCTC CTCAGCTGGG CTGCACTATT AAGCTCCGCG GAGCCTCAGA GTATGAGCTG 
GCACGGGTAA AAGAGATCAT CATTTTTATG GTGTGTGTGG CATACCACTC GCAGCTAGAG 
ATCTCTTTCC TCATGGATGA GTTCGCTATG CCTCCCAGTC TGGCTGAGAG TTCTTCTTTC 
CCATGTCTGC TGGAAAGCAC CACTTTACGG GAAGAGGATG AAACTAGTGG GGAACGGGAA 
AGCCAAGACA ATGATGAGTC TACACTGGGA GATGACAATC TCACACTTGT TCCAGGAGAT 
GACTTTGAGA CAGGATTTAC AGGGACTATC AAAGCCCACA GTCGAGAGCC TTCCACCTCT 
GAATCTTCTC ATAAAGATGG AGAAAGCCCC AGAATAAATA AAAATGCTTC AATTACCTCC 
TTCTCTGGAG GGGAGGAAGA AAATATAAAG ACCTCCACGC CACTTTCCTC CTTCTCTTCC 
ACCTTTCCTC AGCCGGTGAC ACCACCTTTC CTGATATCAG ACGTAAAAGA AATGTCACAG 
GAAGTGGTCA AGGGGTCAGG TGAAGGGGAG AAGAACAAAT ATCTGGAGTC GACTCTGGTC 
CATCAGGACA GCACAAGCTC TGAGACCTCC CTTCCCCCAA CTCGGCTCTT TAGAGACCCC 
TTACAGGACG ACACAGGCCT GTTTGTGACA GAGCATGTAG CTTCTTCAGA TGACCGCCTC 
AAATCCATCT CTGCTTTATT CAAACAGGAG CTAAAAGACA TCATCCTGTG CATTTCACCC 
TTTATTACCT TTCGAGAGCC TTTCTTGCTT ACCCCAGCTG GACTGCGGTG TCCTAGCCGG 
GATTATTTCC CTGAACAGGT TTACCTTTCA CCTCTGCTGA ACAAGGATTC AAAAGAACTG 
GATGGACGCC GCAAGAGGCA GCTGCTGAAA GAGTCCAGCC CAGGTTCTGG TAGCCTGACC 
AATGGTTCCG TTTCACACCA GAGAGCCATT CAGATCTTGC CCTGTCACAA ACTCACTAGT 
GCCCGAATAG CAGAACAGGT AGGCAGCAGT CAGCAGCTGG CACGCATGTT AGCGGACTAC 
CGTGCCCAAG GAGGACGTAT CCGGCAAAGA GAGGGAACAC AATTTCGTGA AGCCCCTCCC 
CCAAAGGCGC CAATAAAGGC AGATAGTGAA GAAGATAAAG GTGCAGGAGT GATTGACATG 
ACCTGGTCTA CTAAGTTGGA CTGTTTAAAT CCAATCAACC ATCAGAGGCT CTGTGTTCTG 
TTCAGTAGCT CTTCAGCACA GTCTAATAAT GCCCCAAACC CCTGCGTCAG TCCCTGGATT 
GTTACAATGG AGTTTTATGG AAAGAATGAC TTGACTCTTG GTGTGTTTCT GGAGAGATAC 
TGTTTTAGAC CATCTTACCA GTGCCCCAGC ATGTACTGTG AGACCCCCAT GGTTCACCAC 
ATCCGGCGCT TTGTGCACGG TAAAGGCTGC GTCCAGATTG TACTGAAAGA GCTGGACTCG 
CCTGTTCCTG GATATCAGCA CACAATCCTC AACTACTCAT GGTGTCGCAT CTGCAAACAG 
GTGACTCCTG TGGTCCCTTT GTCTAATGAC TCTTGGTCCA TGTCCTTCGC CAAGTACCTT 
GAGCTTCGTT TCTATGGTCA TCAGTACACC CGTCGGGCTA ACGCTGAGCC TTGTGGCCAC 
TCTATCCATA AAGATTATCA CCAGTACTTC TCTTACAACC AGATGGTGGC ATCCTTCAGC 
TACATTCCAG TGAGGCTGTT AGAGATCTGT CTGCCTCCCC CGAAGATCAT CATCAGGAAC 
CAAGGTCCAT CGAAATCCAG CTTGCAGCAG GATCTCAAAG ACTTCTCCCA GAAGGTGGCT 
CAGGTGTACC TGGCTATAGA TGACCGTCTT ACCTCCTTAA AAACGGACAC ATTCAGCAAG 
ACCAGAGAGG AAAAAATGGA GGACATGTTT GCACAGAAAG ATATGGAGGA ATCAGAGCTT 
CGGGGCTGGA TAGAGAAGTT ACAAGTGCGT CTGCAGACGA GTGTGATTGA CTCTTCACAA 
CAACTACAGG CTGTTCTGGA GTCAGTGGTG GTCAAAAAAC AGGGCTTGTG TGAAACCCTG 
CAGTCCTGGA ACAACAGACT TCAAGAACTG TTCCAGCAAG AGAAAGGCAG AAAACGTCTA 
TCTGTTCCTC CTAGCCCAGG CAGACACAGA CAACCTACAA CAGATGAGAG CAAGACTAGT 
GCTTTGGAGT CTTCTCCTCG TAACCCTTCC CCAGTGGTCC CAAATGGAGA CAAAGAGGAC 
CGTCATCTCA ATACATTTCC ATCAAGTTCA GGGTCCTCGT CGTTACTACA GTTACCATCT 
CCAGCTGAAC AACCTTCAGA TGTCATTACA AGCGGACCAT CTTTCCCTGA TCAGGACTCT 
GTTAGCAATC CAGAGGACAT GTTTGATGGA CACTTGCTTG GTTCCAATGA TAGTCAGGTA 
AAGGAAAAGT CCACCATGAA AACAATCCTG GCCAACCTGT TACCTGGAAA CAGCTACAAT 
CCCATCCCTT TTCCTTTTGA CCCAGACAAG CACTATCTGA TGTATGAGCA TGAGAGAGTT 
CCTATAGCCG TGTGTGAGAG AGAGCCCAGC TCCATAATTG CCTTTGCACT CAGCTGCAAA 
GAGTATAAAA CTGCCCTTGA AGAGCTCACA AAGACAACAG CAAAGACTGG AGGTGATGAC 
ACTTCTCAGA CCATCAGTGC TGGAGAGAGC AGAGTGAAGA ACAGTCCAGC CAAACCCAGT 
GACAACAGCA TGTCACAGCT GAGCCGCAGC AGCGTCGACG CTGATCCACT CAAGGAGCCT 
GAAAGTGCAG ACAAACAGAA GAAGCAGACA GGGAACCCAC ATATTGAGCT GCAGTTCTCC 
GATGCAAATG CTAAGTTCTA CTGCCGGATC TACTATGCTG AAGAGTTCCA TAAAATGCGG 
GAGGAGATCA TGGAGAGCAC AGAGGATGAT TTTGTGCGAT CTCTTTCCCA CTGTGTCAAC 
TGGCAGGCGC GTGGTGGGAA ATCTGGTGCA GTTTTCTATG CCACTGAAGA TGATCGGTTT 
ATTCTGAAGC AGATGCCCAG ATTAGAGGTC CAGTCCTTTT TAGACTTTGC ACCCCACTAC 
TTTACGTACA TCACAGGAGC GGTTCACCTA AAGCGACCCA CAGCACTTGC TAAGATTCTG 
GGAGTGTACC GCATCGGCTA CAAGAACTCC CAGAACAACA CTGAGAAGAA GCTTGACCTG 
CTTGTGATGG AAAACCTTTT CTATGGACGA AAGATGGCAC AGGTGTTTGA CCTGAAAGGA 
TCGCTGAGAA ACAGGAACGT GAAGACGGAC CAGGGAAAGG AGAGCTGTGA GGTGGTGCTC 
CTAGATGAGA ACCTGCTCAA ACTGGTGCAT GACAATCCCC TTTATATTCG CTCGCACTGC 
AAGGCCATCC TGCGTGCTGC CATCCACAGC GACGCGCTCT TCCTGTCCAG CCACCTCATC 
ATTGATTACT CCCTGCTGGT GGGCCGAGAC GATTCCACAG ATGAGCTAGT TGTGGGGATC 
ATAGATTATA TCCGGACTTT CACTTGGGAT AAAAAGCTGG AGATGGTGGT CAAATCTACT 
GGGATTCTTG GAGGTCAAGG TAAAATGCCC ACAGTGGTGT CACCAGAGCT GTATCGAACA 
CGTTTCTGTG AGGCCATGGA TAAATACTTC CTTATGGTCC CTGATCACTG GAGTGGACTT 
GGGCTCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167722.1
CDS	164..6478
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASAEWEEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167665.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167665.4

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGCG GAGTGGGAAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAATGGA AGTCTTGCTC 
TCCGAAGATG GAGGTCAGCA TATATCCATC AGTGATGCAT TCATCAAAGA GTCTTTGTTC 
AACCGGAGGG TTGAGGAGAA AGCTAAAGAG ATGCTCTTCA CTCCTCTCGG CTGGCATCAC 
AGCTCTCTGG ATCAGCTGCG GGAAGAGAAT GGGGAAAAGA AAGCGATGGA GCGGTTGCTG 
TCAGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAAC TGCTGTACAG TGAATCACTG 
TCTCTGTCGT GGCGTGACAT CATCGTACCT GTGGTGAGGC AGGTGGTGCA GACGGTGCGA 
CCGGATGTGC GCAGCTGTGA CGATGATATG GACATCCGTC AGTTTGTCCA CATCAAGAAG 
ATTCCAGGAG GAAAGAAGTT TGACTCTGCT GTAGTGAATG GTTTTGTTTG CACAAAGAAT 
ATCGCACACA AAAAGATGAA CTCTTACATC AAAAACCCCA AGATCCTTCT TCTCAAATGC 
TCCATTGAGT ATCTATATAG AGAGGAGACC AAGTTCACCT GCATTGACCC AATTGTTTTA 
CAGGAGCGTG AGTTTCTTAA GAACTATGTT CAGAGGATTG TTGATGTCCG ACCAAACTTA 
GTCCTGGTGG AGAAGACTGT ATCGCGAATT GCTCAGGACA TGCTACTAGA GCACGGCATT 
GCCATTGTGA TTAATGTCAA ACCTCAAGTA CTGGACCGTG TGAGTCGAAT GACTCAGGGA 
GATTTAGTCA TTTCAATGGA TCAGCTTCTT ACAAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACTTGC ACTCCTTCCA GCTACCAAAT AGTGAAGTGA AAACCCTGAT GTTTTTTGAG 
GGCTGTCCTC CTCAGCTGGG CTGCACTATT AAGCTCCGCG GAGCCTCAGA GTATGAGCTG 
GCACGGGTAA AAGAGATCAT CATTTTTATG GTGTGTGTGG CATACCACTC GCAGCTAGAG 
ATCTCTTTCC TCATGGATGA GTTCGCTATG CCTCCCAGTC TGGCTGAGAG TTCTTCTTTC 
CCATGTCTGC TGGAAAGCAC CACTTTACGG GAAGAGGATG AAACTAGTGG GGAACGGGAA 
AGCCAAGACA ATGATGAGTC TACACTGGGA GATGACAATC TCACACTTGT TCCAGGAGAT 
GACTTTGAGA CAGGATTTAC AGGGACTATC AAAGCCCACA GTCGAGAGCC TTCCACCTCT 
GAATCTTCTC ATAAAGATGG AGAAAGCCCC AGAATAAATA AAAATGCTTC AATTACCTCC 
TTCTCTGGAG GGGAGGAAGA AAATATAAAG ACCTCCACGC CACTTTCCTC CTTCTCTTCC 
ACCTTTCCTC AGCCGGTGAC ACCACCTTTC CTGATATCAG ACGTAAAAGA AATGTCACAG 
GAAGTGGTCA AGGGGTCAGG TGAAGGGGAG AAGAACAAAT ATCTGGAGTC GACTCTGGTC 
CATCAGGACA GCACAAGCTC TGAGACCTCC CTTCCCCCAA CTCGGCTCTT TAGAGACCCC 
TTACAGGACG ACACAGGCCT GTTTGTGACA GAGCATGTAG CTTCTTCAGA TGACCGCCTC 
AAATCCATCT CTGCTTTATT CAAACAGGAG CTAAAAGACA TCATCCTGTG CATTTCACCC 
TTTATTACCT TTCGAGAGCC TTTCTTGCTT ACCCCAGCTG GACTGCGGTG TCCTAGCCGG 
GATTATTTCC CTGAACAGGT TTACCTTTCA CCTCTGCTGA ACAAGGATTC AAAAGAACTG 
GATGGACGCC GCAAGAGGCA GCTGCTGAAA GAGTCCAGCC CAGGTTCTGG TAGCCTGACC 
AATGGTTCCG TTTCACACCA GAGAGCCATT CAGATCTTGC CCTGTCACAA ACTCACTAGT 
GCCCGAATAG CAGAACAGGT AGGCAGCAGT CAGCAGCTGG CACGCATGTT AGCGGACTAC 
CGTGCCCAAG GAGGACGTAT CCGGCAAAGA GAGGGAACAC AATTTCGTGA AGCCCCTCCC 
CCAAAGGCGC CAATAAAGGC AGATAGTGAA GAAGATAAAG GTGCAGGAGT GATTGACATG 
ACCTGGTCTA CTAAGTTGGA CTGTTTAAAT CCAATCAACC ATCAGAGGCT CTGTGTTCTG 
TTCAGTAGCT CTTCAGCACA GTCTAATAAT GCCCCAAACC CCTGCGTCAG TCCCTGGATT 
GTTACAATGG AGTTTTATGG AAAGAATGAC TTGACTCTTG GTGTGTTTCT GGAGAGATAC 
TGTTTTAGAC CATCTTACCA GTGCCCCAGC ATGTACTGTG AGACCCCCAT GGTTCACCAC 
ATCCGGCGCT TTGTGCACGG TAAAGGCTGC GTCCAGATTG TACTGAAAGA GCTGGACTCG 
CCTGTTCCTG GATATCAGCA CACAATCCTC AACTACTCAT GGTGTCGCAT CTGCAAACAG 
GTGACTCCTG TGGTCCCTTT GTCTAATGAC TCTTGGTCCA TGTCCTTCGC CAAGTACCTT 
GAGCTTCGTT TCTATGGTCA TCAGTACACC CGTCGGGCTA ACGCTGAGCC TTGTGGCCAC 
TCTATCCATA AAGATTATCA CCAGTACTTC TCTTACAACC AGATGGTGGC ATCCTTCAGC 
TACATTCCAG TGAGGCTGTT AGAGATCTGT CTGCCTCCCC CGAAGATCAT CATCAGGAAC 
CAAGGTCCAT CGAAATCCAG CTTGCAGCAG GATCTCAAAG ACTTCTCCCA GAAGGTGGCT 
CAGGTGTACC TGGCTATAGA TGACCGTCTT ACCTCCTTAA AAACGGACAC ATTCAGCAAG 
ACCAGAGAGG AAAAAATGGA GGACATGTTT GCACAGAAAG ATATGGAGGA ATCAGAGCTT 
CGGGGCTGGA TAGAGAAGTT ACAAGTGCGT CTGCAGACGA GTGTGATTGA CTCTTCACAA 
CAACTACAGG CTGTTCTGGA GTCAGTGGTG GTCAAAAAAC AGGGCTTGTG TGAAACCCTG 
CAGTCCTGGA ACAACAGACT TCAAGAACTG TTCCAGCAAG AGAAAGGCAG AAAACGTCTA 
TCTGTTCCTC CTAGCCCAGG CAGACACAGA CAACCTACAA CAGATGAGAG CAAGACTAGT 
GCTTTGGAGT CTTCTCCTCG TAACCCTTCC CCAGTGGTCC CAAATGGAGA CAAAGAGGAC 
CGTCATCTCA ATACATTTCC ATCAAGTTCA GGGTCCTCGT CGTTACTACA GTTACCATCT 
CCAGCTGAAC AACCTTCAGA TGTCATTACA AGCGGACCAT CTTTCCCTGA TCAGGACTCT 
GTTAGCAATC CAGAGGACAT GTTTGATGGA CACTTGCTTG GTTCCAATGA TAGTCAGGTA 
AAGGAAAAGT CCACCATGAA AACAATCCTG GCCAACCTGT TACCTGGAAA CAGCTACAAT 
CCCATCCCTT TTCCTTTTGA CCCAGACAAG CACTATCTGA TGTATGAGCA TGAGAGAGTT 
CCTATAGCCG TGTGTGAGAG AGAGCCCAGC TCCATAATTG CCTTTGCACT CAGCTGCAAA 
GAGTATAAAA CTGCCCTTGA AGAGCTCACA AAGACAACAG CAAAGACTGG AGGTGATGAC 
ACTTCTCAGA CCATCAGTGC TGGAGAGAGC AGAGTGAAGA ACAGTCCAGC CAAACCCAGT 
GACAACAGCA TGTCACAGCT GAGCCGCAGC AGCGTCGACG CTGATCCACT CAAGGAGCCT 
GAAAGTGCAG ACAAACAGAA GAAGCAGACA GGGAACCCAC ATATTGAGCT GCAGTTCTCC 
GATGCAAATG CTAAGTTCTA CTGCCGGATC TACTATGCTG AAGAGTTCCA TAAAATGCGG 
GAGGAGATCA TGGAGAGCAC AGAGGATGAT TTTGTGCGAT CTCTTTCCCA CTGTGTCAAC 
TGGCAGGCGC GTGGTGGGAA ATCTGGTGCA GTTTTCTATG CCACTGAAGA TGATCGGTTT 
ATTCTGAAGC AGATGCCCAG ATTAGAGGTC CAGTCCTTTT TAGACTTTGC ACCCCACTAC 
TTTACGTACA TCACAGGAGC GGTTCACCTA AAGCGACCCA CAGCACTTGC TAAGATTCTG 
GGAGTGTACC GCATCGGCTA CAAGAACTCC CAGAACAACA CTGAGAAGAA GCTTGACCTG 
CTTGTGATGG AAAACCTTTT CTATGGACGA AAGATGGCAC AGGTGTTTGA CCTGAAAGGA 
TCGCTGAGAA ACAGGAACGT GAAGACGGAC CAGGGAAAGG AGAGCTGTGA GGTGGTGCTC 
CTAGATGAGA ACCTGCTCAA ACTGGTGCAT GACAATCCCC TTTATATTCG CTCGCACTGC 
AAGGCCATCC TGCGTGCTGC CATCCACAGC GACGCGCTCT TCCTGTCCAG CCACCTCATC 
ATTGATTACT CCCTGCTGGT GGGCCGAGAC GATTCCACAG ATGAGCTAGT TGTGGGGATC 
ATAGATTATA TCCGGACTTT CACTTGGGAT AAAAAGCTGG AGATGGTGGT CAAATCTACT 
GGGATTCTTG GAGGTCAAGG TAAAATGCCC ACAGTGGTGT CACCAGAGCT GTATCGAACA 
CGTTTCTGTG AGGCCATGGA TAAATACTTC CTTATGGTCC CTGATCACTG GAGTGGACTT 
GGGCTCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167666.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167723.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.

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CloneID	ODa15216
Clone ID Related Accession (Same CDS sequence)	XM_005167666.3 , NM_001127305.1 , XM_005167666.4
Accession Version	XM_005167666.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAATGGAAG TCTTGCTCTC CGAAGATGGA 
GGTCAGCATA TATCCATCAG TGATGCATTC ATCAAAGAGT CTTTGTTCAA CCGGAGGGTT 
GAGGAGAAAG CTAAAGAGAT GCTCTTCACT CCTCTCGGCT GGCATCACAG CTCTCTGGAT 
CAGCTGCGGG AAGAGAATGG GGAAAAGAAA GCGATGGAGC GGTTGCTGTC AGCAAATCAC 
AGCCACATGA TGGCGCTGCT GCAGCAACTG CTGTACAGTG AATCACTGTC TCTGTCGTGG 
CGTGACATCA TCGTACCTGT GGTGAGGCAG GTGGTGCAGA CGGTGCGACC GGATGTGCGC 
AGCTGTGACG ATGATATGGA CATCCGTCAG TTTGTCCACA TCAAGAAGAT TCCAGGAGGA 
AAGAAGTTTG ACTCTGCTGT AGTGAATGGT TTTGTTTGCA CAAAGAATAT CGCACACAAA 
AAGATGAACT CTTACATCAA AAACCCCAAG ATCCTTCTTC TCAAATGCTC CATTGAGTAT 
CTATATAGAG AGGAGACCAA GTTCACCTGC ATTGACCCAA TTGTTTTACA GGAGCGTGAG 
TTTCTTAAGA ACTATGTTCA GAGGATTGTT GATGTCCGAC CAAACTTAGT CCTGGTGGAG 
AAGACTGTAT CGCGAATTGC TCAGGACATG CTACTAGAGC ACGGCATTGC CATTGTGATT 
AATGTCAAAC CTCAAGTACT GGACCGTGTG AGTCGAATGA CTCAGGGAGA TTTAGTCATT 
TCAATGGATC AGCTTCTTAC AAAACCTCGT CTGGGTACCT GCCACAAGTT TTACTTGCAC 
TCCTTCCAGC TACCAAATAG TGAAGTGAAA ACCCTGATGT TTTTTGAGGG CTGTCCTCCT 
CAGCTGGGCT GCACTATTAA GCTCCGCGGA GCCTCAGAGT ATGAGCTGGC ACGGGTAAAA 
GAGATCATCA TTTTTATGGT GTGTGTGGCA TACCACTCGC AGCTAGAGAT CTCTTTCCTC 
ATGGATGAGT TCGCTATGCC TCCCAGTCTG GCTGAGAGTT CTTCTTTCCC ATGTCTGCTG 
GAAAGCACCA CTTTACGGGA AGAGGATGAA ACTAGTGGGG AACGGGAAAG CCAAGACAAT 
GATGAGTCTA CACTGGGAGA TGACAATCTC ACACTTGTTC CAGGAGATGA CTTTGAGACA 
GGATTTACAG GGACTATCAA AGCCCACAGT CGAGAGCCTT CCACCTCTGA ATCTTCTCAT 
AAAGATGGAG AAAGCCCCAG AATAAATAAA AATGCTTCAA TTACCTCCTT CTCTGGAGGG 
GAGGAAGAAA ATATAAAGAC CTCCACGCCA CTTTCCTCCT TCTCTTCCAC CTTTCCTCAG 
CCGGTGACAC CACCTTTCCT GATATCAGAC GTAAAAGAAA TGTCACAGGA AGTGGTCAAG 
GGGTCAGGTG AAGGGGAGAA GAACAAATAT CTGGAGTCGA CTCTGGTCCA TCAGGACAGC 
ACAAGCTCTG AGACCTCCCT TCCCCCAACT CGGCTCTTTA GAGACCCCTT ACAGGACGAC 
ACAGGCCTGT TTGTGACAGA GCATGTAGCT TCTTCAGATG ACCGCCTCAA ATCCATCTCT 
GCTTTATTCA AACAGGAGCT AAAAGACATC ATCCTGTGCA TTTCACCCTT TATTACCTTT 
CGAGAGCCTT TCTTGCTTAC CCCAGCTGGA CTGCGGTGTC CTAGCCGGGA TTATTTCCCT 
GAACAGGTTT ACCTTTCACC TCTGCTGAAC AAGGATTCAA AAGAACTGGA TGGACGCCGC 
AAGAGGCAGC TGCTGAAAGA GTCCAGCCCA GGTTCTGGTA GCCTGACCAA TGGTTCCGTT 
TCACACCAGA GAGCCATTCA GATCTTGCCC TGTCACAAAC TCACTAGTGC CCGAATAGCA 
GAACAGGTAG GCAGCAGTCA GCAGCTGGCA CGCATGTTAG CGGACTACCG TGCCCAAGGA 
GGACGTATCC GGCAAAGAGA GGGAACACAA TTTCGTGAAG CCCCTCCCCC AAAGGCGCCA 
ATAAAGGCAG ATAGTGAAGA AGATAAAGGT GCAGGAGTGA TTGACATGAC CTGGTCTACT 
AAGTTGGACT GTTTAAATCC AATCAACCAT CAGAGGCTCT GTGTTCTGTT CAGTAGCTCT 
TCAGCACAGT CTAATAATGC CCCAAACCCC TGCGTCAGTC CCTGGATTGT TACAATGGAG 
TTTTATGGAA AGAATGACTT GACTCTTGGT GTGTTTCTGG AGAGATACTG TTTTAGACCA 
TCTTACCAGT GCCCCAGCAT GTACTGTGAG ACCCCCATGG TTCACCACAT CCGGCGCTTT 
GTGCACGGTA AAGGCTGCGT CCAGATTGTA CTGAAAGAGC TGGACTCGCC TGTTCCTGGA 
TATCAGCACA CAATCCTCAA CTACTCATGG TGTCGCATCT GCAAACAGGT GACTCCTGTG 
GTCCCTTTGT CTAATGACTC TTGGTCCATG TCCTTCGCCA AGTACCTTGA GCTTCGTTTC 
TATGGTCATC AGTACACCCG TCGGGCTAAC GCTGAGCCTT GTGGCCACTC TATCCATAAA 
GATTATCACC AGTACTTCTC TTACAACCAG ATGGTGGCAT CCTTCAGCTA CATTCCAGTG 
AGGCTGTTAG AGATCTGTCT GCCTCCCCCG AAGATCATCA TCAGGAACCA AGGTCCATCG 
AAATCCAGCT TGCAGCAGGA TCTCAAAGAC TTCTCCCAGA AGGTGGCTCA GGTGTACCTG 
GCTATAGATG ACCGTCTTAC CTCCTTAAAA ACGGACACAT TCAGCAAGAC CAGAGAGGAA 
AAAATGGAGG ACATGTTTGC ACAGAAAGAT ATGGAGGAAT CAGAGCTTCG GGGCTGGATA 
GAGAAGTTAC AAGTGCGTCT GCAGACGAGT GTGATTGACT CTTCACAACA ACTACAGGCT 
GTTCTGGAGT CAGTGGTGGT CAAAAAACAG GGCTTGTGTG AAACCCTGCA GTCCTGGAAC 
AACAGACTTC AAGAACTGTT CCAGCAAGAG AAAGGCAGAA AACGTCTATC TGTTCCTCCT 
AGCCCAGGCA GACACAGACA ACCTACAACA GATGAGAGCA AGACTAGTGC TTTGGAGTCT 
TCTCCTCGTA ACCCTTCCCC AGTGGTCCCA AATGGAGACA AAGAGGACCG TCATCTCAAT 
ACATTTCCAT CAAGTTCAGG GTCCTCGTCG TTACTACAGT TACCATCTCC AGCTGAACAA 
CCTTCAGATG TCATTACAAG CGGACCATCT TTCCCTGATC AGGACTCTGT TAGCAATCCA 
GAGGACATGT TTGATGGACA CTTGCTTGGT TCCAATGATA GTCAGGTAAA GGAAAAGTCC 
ACCATGAAAA CAATCCTGGC CAACCTGTTA CCTGGAAACA GCTACAATCC CATCCCTTTT 
CCTTTTGACC CAGACAAGCA CTATCTGATG TATGAGCATG AGAGAGTTCC TATAGCCGTG 
TGTGAGAGAG AGCCCAGCTC CATAATTGCC TTTGCACTCA GCTGCAAAGA GTATAAAACT 
GCCCTTGAAG AGCTCACAAA GACAACAGCA AAGACTGGAG GTGATGACAC TTCTCAGACC 
ATCAGTGCTG GAGAGAGCAG AGTGAAGAAC AGTCCAGCCA AACCCAGTGA CAACAGCATG 
TCACAGCTGA GCCGCAGCAG CGTCGACGCT GATCCACTCA AGGAGCCTGA AAGTGCAGAC 
AAACAGAAGA AGCAGACAGG GAACCCACAT ATTGAGCTGC AGTTCTCCGA TGCAAATGCT 
AAGTTCTACT GCCGGATCTA CTATGCTGAA GAGTTCCATA AAATGCGGGA GGAGATCATG 
GAGAGCACAG AGGATGATTT TGTGCGATCT CTTTCCCACT GTGTCAACTG GCAGGCGCGT 
GGTGGGAAAT CTGGTGCAGT TTTCTATGCC ACTGAAGATG ATCGGTTTAT TCTGAAGCAG 
ATGCCCAGAT TAGAGGTCCA GTCCTTTTTA GACTTTGCAC CCCACTACTT TACGTACATC 
ACAGGAGCGG TTCACCTAAA GCGACCCACA GCACTTGCTA AGATTCTGGG AGTGTACCGC 
ATCGGCTACA AGAACTCCCA GAACAACACT GAGAAGAAGC TTGACCTGCT TGTGATGGAA 
AACCTTTTCT ATGGACGAAA GATGGCACAG GTGTTTGACC TGAAAGGATC GCTGAGAAAC 
AGGAACGTGA AGACGGACCA GGGAAAGGAG AGCTGTGAGG TGGTGCTCCT AGATGAGAAC 
CTGCTCAAAC TGGTGCATGA CAATCCCCTT TATATTCGCT CGCACTGCAA GGCCATCCTG 
CGTGCTGCCA TCCACAGCGA CGCGCTCTTC CTGTCCAGCC ACCTCATCAT TGATTACTCC 
CTGCTGGTGG GCCGAGACGA TTCCACAGAT GAGCTAGTTG TGGGGATCAT AGATTATATC 
CGGACTTTCA CTTGGGATAA AAAGCTGGAG ATGGTGGTCA AATCTACTGG GATTCTTGGA 
GGTCAAGGTA AAATGCCCAC AGTGGTGTCA CCAGAGCTGT ATCGAACACG TTTCTGTGAG 
GCCATGGATA AATACTTCCT TATGGTCCCT GATCACTGGA GTGGACTTGG GCTCAACTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167723.1
CDS	156..6458
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167666.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167666.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAATGGAAG TCTTGCTCTC CGAAGATGGA 
GGTCAGCATA TATCCATCAG TGATGCATTC ATCAAAGAGT CTTTGTTCAA CCGGAGGGTT 
GAGGAGAAAG CTAAAGAGAT GCTCTTCACT CCTCTCGGCT GGCATCACAG CTCTCTGGAT 
CAGCTGCGGG AAGAGAATGG GGAAAAGAAA GCGATGGAGC GGTTGCTGTC AGCAAATCAC 
AGCCACATGA TGGCGCTGCT GCAGCAACTG CTGTACAGTG AATCACTGTC TCTGTCGTGG 
CGTGACATCA TCGTACCTGT GGTGAGGCAG GTGGTGCAGA CGGTGCGACC GGATGTGCGC 
AGCTGTGACG ATGATATGGA CATCCGTCAG TTTGTCCACA TCAAGAAGAT TCCAGGAGGA 
AAGAAGTTTG ACTCTGCTGT AGTGAATGGT TTTGTTTGCA CAAAGAATAT CGCACACAAA 
AAGATGAACT CTTACATCAA AAACCCCAAG ATCCTTCTTC TCAAATGCTC CATTGAGTAT 
CTATATAGAG AGGAGACCAA GTTCACCTGC ATTGACCCAA TTGTTTTACA GGAGCGTGAG 
TTTCTTAAGA ACTATGTTCA GAGGATTGTT GATGTCCGAC CAAACTTAGT CCTGGTGGAG 
AAGACTGTAT CGCGAATTGC TCAGGACATG CTACTAGAGC ACGGCATTGC CATTGTGATT 
AATGTCAAAC CTCAAGTACT GGACCGTGTG AGTCGAATGA CTCAGGGAGA TTTAGTCATT 
TCAATGGATC AGCTTCTTAC AAAACCTCGT CTGGGTACCT GCCACAAGTT TTACTTGCAC 
TCCTTCCAGC TACCAAATAG TGAAGTGAAA ACCCTGATGT TTTTTGAGGG CTGTCCTCCT 
CAGCTGGGCT GCACTATTAA GCTCCGCGGA GCCTCAGAGT ATGAGCTGGC ACGGGTAAAA 
GAGATCATCA TTTTTATGGT GTGTGTGGCA TACCACTCGC AGCTAGAGAT CTCTTTCCTC 
ATGGATGAGT TCGCTATGCC TCCCAGTCTG GCTGAGAGTT CTTCTTTCCC ATGTCTGCTG 
GAAAGCACCA CTTTACGGGA AGAGGATGAA ACTAGTGGGG AACGGGAAAG CCAAGACAAT 
GATGAGTCTA CACTGGGAGA TGACAATCTC ACACTTGTTC CAGGAGATGA CTTTGAGACA 
GGATTTACAG GGACTATCAA AGCCCACAGT CGAGAGCCTT CCACCTCTGA ATCTTCTCAT 
AAAGATGGAG AAAGCCCCAG AATAAATAAA AATGCTTCAA TTACCTCCTT CTCTGGAGGG 
GAGGAAGAAA ATATAAAGAC CTCCACGCCA CTTTCCTCCT TCTCTTCCAC CTTTCCTCAG 
CCGGTGACAC CACCTTTCCT GATATCAGAC GTAAAAGAAA TGTCACAGGA AGTGGTCAAG 
GGGTCAGGTG AAGGGGAGAA GAACAAATAT CTGGAGTCGA CTCTGGTCCA TCAGGACAGC 
ACAAGCTCTG AGACCTCCCT TCCCCCAACT CGGCTCTTTA GAGACCCCTT ACAGGACGAC 
ACAGGCCTGT TTGTGACAGA GCATGTAGCT TCTTCAGATG ACCGCCTCAA ATCCATCTCT 
GCTTTATTCA AACAGGAGCT AAAAGACATC ATCCTGTGCA TTTCACCCTT TATTACCTTT 
CGAGAGCCTT TCTTGCTTAC CCCAGCTGGA CTGCGGTGTC CTAGCCGGGA TTATTTCCCT 
GAACAGGTTT ACCTTTCACC TCTGCTGAAC AAGGATTCAA AAGAACTGGA TGGACGCCGC 
AAGAGGCAGC TGCTGAAAGA GTCCAGCCCA GGTTCTGGTA GCCTGACCAA TGGTTCCGTT 
TCACACCAGA GAGCCATTCA GATCTTGCCC TGTCACAAAC TCACTAGTGC CCGAATAGCA 
GAACAGGTAG GCAGCAGTCA GCAGCTGGCA CGCATGTTAG CGGACTACCG TGCCCAAGGA 
GGACGTATCC GGCAAAGAGA GGGAACACAA TTTCGTGAAG CCCCTCCCCC AAAGGCGCCA 
ATAAAGGCAG ATAGTGAAGA AGATAAAGGT GCAGGAGTGA TTGACATGAC CTGGTCTACT 
AAGTTGGACT GTTTAAATCC AATCAACCAT CAGAGGCTCT GTGTTCTGTT CAGTAGCTCT 
TCAGCACAGT CTAATAATGC CCCAAACCCC TGCGTCAGTC CCTGGATTGT TACAATGGAG 
TTTTATGGAA AGAATGACTT GACTCTTGGT GTGTTTCTGG AGAGATACTG TTTTAGACCA 
TCTTACCAGT GCCCCAGCAT GTACTGTGAG ACCCCCATGG TTCACCACAT CCGGCGCTTT 
GTGCACGGTA AAGGCTGCGT CCAGATTGTA CTGAAAGAGC TGGACTCGCC TGTTCCTGGA 
TATCAGCACA CAATCCTCAA CTACTCATGG TGTCGCATCT GCAAACAGGT GACTCCTGTG 
GTCCCTTTGT CTAATGACTC TTGGTCCATG TCCTTCGCCA AGTACCTTGA GCTTCGTTTC 
TATGGTCATC AGTACACCCG TCGGGCTAAC GCTGAGCCTT GTGGCCACTC TATCCATAAA 
GATTATCACC AGTACTTCTC TTACAACCAG ATGGTGGCAT CCTTCAGCTA CATTCCAGTG 
AGGCTGTTAG AGATCTGTCT GCCTCCCCCG AAGATCATCA TCAGGAACCA AGGTCCATCG 
AAATCCAGCT TGCAGCAGGA TCTCAAAGAC TTCTCCCAGA AGGTGGCTCA GGTGTACCTG 
GCTATAGATG ACCGTCTTAC CTCCTTAAAA ACGGACACAT TCAGCAAGAC CAGAGAGGAA 
AAAATGGAGG ACATGTTTGC ACAGAAAGAT ATGGAGGAAT CAGAGCTTCG GGGCTGGATA 
GAGAAGTTAC AAGTGCGTCT GCAGACGAGT GTGATTGACT CTTCACAACA ACTACAGGCT 
GTTCTGGAGT CAGTGGTGGT CAAAAAACAG GGCTTGTGTG AAACCCTGCA GTCCTGGAAC 
AACAGACTTC AAGAACTGTT CCAGCAAGAG AAAGGCAGAA AACGTCTATC TGTTCCTCCT 
AGCCCAGGCA GACACAGACA ACCTACAACA GATGAGAGCA AGACTAGTGC TTTGGAGTCT 
TCTCCTCGTA ACCCTTCCCC AGTGGTCCCA AATGGAGACA AAGAGGACCG TCATCTCAAT 
ACATTTCCAT CAAGTTCAGG GTCCTCGTCG TTACTACAGT TACCATCTCC AGCTGAACAA 
CCTTCAGATG TCATTACAAG CGGACCATCT TTCCCTGATC AGGACTCTGT TAGCAATCCA 
GAGGACATGT TTGATGGACA CTTGCTTGGT TCCAATGATA GTCAGGTAAA GGAAAAGTCC 
ACCATGAAAA CAATCCTGGC CAACCTGTTA CCTGGAAACA GCTACAATCC CATCCCTTTT 
CCTTTTGACC CAGACAAGCA CTATCTGATG TATGAGCATG AGAGAGTTCC TATAGCCGTG 
TGTGAGAGAG AGCCCAGCTC CATAATTGCC TTTGCACTCA GCTGCAAAGA GTATAAAACT 
GCCCTTGAAG AGCTCACAAA GACAACAGCA AAGACTGGAG GTGATGACAC TTCTCAGACC 
ATCAGTGCTG GAGAGAGCAG AGTGAAGAAC AGTCCAGCCA AACCCAGTGA CAACAGCATG 
TCACAGCTGA GCCGCAGCAG CGTCGACGCT GATCCACTCA AGGAGCCTGA AAGTGCAGAC 
AAACAGAAGA AGCAGACAGG GAACCCACAT ATTGAGCTGC AGTTCTCCGA TGCAAATGCT 
AAGTTCTACT GCCGGATCTA CTATGCTGAA GAGTTCCATA AAATGCGGGA GGAGATCATG 
GAGAGCACAG AGGATGATTT TGTGCGATCT CTTTCCCACT GTGTCAACTG GCAGGCGCGT 
GGTGGGAAAT CTGGTGCAGT TTTCTATGCC ACTGAAGATG ATCGGTTTAT TCTGAAGCAG 
ATGCCCAGAT TAGAGGTCCA GTCCTTTTTA GACTTTGCAC CCCACTACTT TACGTACATC 
ACAGGAGCGG TTCACCTAAA GCGACCCACA GCACTTGCTA AGATTCTGGG AGTGTACCGC 
ATCGGCTACA AGAACTCCCA GAACAACACT GAGAAGAAGC TTGACCTGCT TGTGATGGAA 
AACCTTTTCT ATGGACGAAA GATGGCACAG GTGTTTGACC TGAAAGGATC GCTGAGAAAC 
AGGAACGTGA AGACGGACCA GGGAAAGGAG AGCTGTGAGG TGGTGCTCCT AGATGAGAAC 
CTGCTCAAAC TGGTGCATGA CAATCCCCTT TATATTCGCT CGCACTGCAA GGCCATCCTG 
CGTGCTGCCA TCCACAGCGA CGCGCTCTTC CTGTCCAGCC ACCTCATCAT TGATTACTCC 
CTGCTGGTGG GCCGAGACGA TTCCACAGAT GAGCTAGTTG TGGGGATCAT AGATTATATC 
CGGACTTTCA CTTGGGATAA AAAGCTGGAG ATGGTGGTCA AATCTACTGG GATTCTTGGA 
GGTCAAGGTA AAATGCCCAC AGTGGTGTCA CCAGAGCTGT ATCGAACACG TTTCTGTGAG 
GCCATGGATA AATACTTCCT TATGGTCCCT GATCACTGGA GTGGACTTGG GCTCAACTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167666.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167723.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.

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CloneID	ODa15216
Clone ID Related Accession (Same CDS sequence)	XM_005167666.3 , NM_001127305.1 , XM_005167666.4
Accession Version	XM_005167666.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167666.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAATGGAAG TCTTGCTCTC CGAAGATGGA 
GGTCAGCATA TATCCATCAG TGATGCATTC ATCAAAGAGT CTTTGTTCAA CCGGAGGGTT 
GAGGAGAAAG CTAAAGAGAT GCTCTTCACT CCTCTCGGCT GGCATCACAG CTCTCTGGAT 
CAGCTGCGGG AAGAGAATGG GGAAAAGAAA GCGATGGAGC GGTTGCTGTC AGCAAATCAC 
AGCCACATGA TGGCGCTGCT GCAGCAACTG CTGTACAGTG AATCACTGTC TCTGTCGTGG 
CGTGACATCA TCGTACCTGT GGTGAGGCAG GTGGTGCAGA CGGTGCGACC GGATGTGCGC 
AGCTGTGACG ATGATATGGA CATCCGTCAG TTTGTCCACA TCAAGAAGAT TCCAGGAGGA 
AAGAAGTTTG ACTCTGCTGT AGTGAATGGT TTTGTTTGCA CAAAGAATAT CGCACACAAA 
AAGATGAACT CTTACATCAA AAACCCCAAG ATCCTTCTTC TCAAATGCTC CATTGAGTAT 
CTATATAGAG AGGAGACCAA GTTCACCTGC ATTGACCCAA TTGTTTTACA GGAGCGTGAG 
TTTCTTAAGA ACTATGTTCA GAGGATTGTT GATGTCCGAC CAAACTTAGT CCTGGTGGAG 
AAGACTGTAT CGCGAATTGC TCAGGACATG CTACTAGAGC ACGGCATTGC CATTGTGATT 
AATGTCAAAC CTCAAGTACT GGACCGTGTG AGTCGAATGA CTCAGGGAGA TTTAGTCATT 
TCAATGGATC AGCTTCTTAC AAAACCTCGT CTGGGTACCT GCCACAAGTT TTACTTGCAC 
TCCTTCCAGC TACCAAATAG TGAAGTGAAA ACCCTGATGT TTTTTGAGGG CTGTCCTCCT 
CAGCTGGGCT GCACTATTAA GCTCCGCGGA GCCTCAGAGT ATGAGCTGGC ACGGGTAAAA 
GAGATCATCA TTTTTATGGT GTGTGTGGCA TACCACTCGC AGCTAGAGAT CTCTTTCCTC 
ATGGATGAGT TCGCTATGCC TCCCAGTCTG GCTGAGAGTT CTTCTTTCCC ATGTCTGCTG 
GAAAGCACCA CTTTACGGGA AGAGGATGAA ACTAGTGGGG AACGGGAAAG CCAAGACAAT 
GATGAGTCTA CACTGGGAGA TGACAATCTC ACACTTGTTC CAGGAGATGA CTTTGAGACA 
GGATTTACAG GGACTATCAA AGCCCACAGT CGAGAGCCTT CCACCTCTGA ATCTTCTCAT 
AAAGATGGAG AAAGCCCCAG AATAAATAAA AATGCTTCAA TTACCTCCTT CTCTGGAGGG 
GAGGAAGAAA ATATAAAGAC CTCCACGCCA CTTTCCTCCT TCTCTTCCAC CTTTCCTCAG 
CCGGTGACAC CACCTTTCCT GATATCAGAC GTAAAAGAAA TGTCACAGGA AGTGGTCAAG 
GGGTCAGGTG AAGGGGAGAA GAACAAATAT CTGGAGTCGA CTCTGGTCCA TCAGGACAGC 
ACAAGCTCTG AGACCTCCCT TCCCCCAACT CGGCTCTTTA GAGACCCCTT ACAGGACGAC 
ACAGGCCTGT TTGTGACAGA GCATGTAGCT TCTTCAGATG ACCGCCTCAA ATCCATCTCT 
GCTTTATTCA AACAGGAGCT AAAAGACATC ATCCTGTGCA TTTCACCCTT TATTACCTTT 
CGAGAGCCTT TCTTGCTTAC CCCAGCTGGA CTGCGGTGTC CTAGCCGGGA TTATTTCCCT 
GAACAGGTTT ACCTTTCACC TCTGCTGAAC AAGGATTCAA AAGAACTGGA TGGACGCCGC 
AAGAGGCAGC TGCTGAAAGA GTCCAGCCCA GGTTCTGGTA GCCTGACCAA TGGTTCCGTT 
TCACACCAGA GAGCCATTCA GATCTTGCCC TGTCACAAAC TCACTAGTGC CCGAATAGCA 
GAACAGGTAG GCAGCAGTCA GCAGCTGGCA CGCATGTTAG CGGACTACCG TGCCCAAGGA 
GGACGTATCC GGCAAAGAGA GGGAACACAA TTTCGTGAAG CCCCTCCCCC AAAGGCGCCA 
ATAAAGGCAG ATAGTGAAGA AGATAAAGGT GCAGGAGTGA TTGACATGAC CTGGTCTACT 
AAGTTGGACT GTTTAAATCC AATCAACCAT CAGAGGCTCT GTGTTCTGTT CAGTAGCTCT 
TCAGCACAGT CTAATAATGC CCCAAACCCC TGCGTCAGTC CCTGGATTGT TACAATGGAG 
TTTTATGGAA AGAATGACTT GACTCTTGGT GTGTTTCTGG AGAGATACTG TTTTAGACCA 
TCTTACCAGT GCCCCAGCAT GTACTGTGAG ACCCCCATGG TTCACCACAT CCGGCGCTTT 
GTGCACGGTA AAGGCTGCGT CCAGATTGTA CTGAAAGAGC TGGACTCGCC TGTTCCTGGA 
TATCAGCACA CAATCCTCAA CTACTCATGG TGTCGCATCT GCAAACAGGT GACTCCTGTG 
GTCCCTTTGT CTAATGACTC TTGGTCCATG TCCTTCGCCA AGTACCTTGA GCTTCGTTTC 
TATGGTCATC AGTACACCCG TCGGGCTAAC GCTGAGCCTT GTGGCCACTC TATCCATAAA 
GATTATCACC AGTACTTCTC TTACAACCAG ATGGTGGCAT CCTTCAGCTA CATTCCAGTG 
AGGCTGTTAG AGATCTGTCT GCCTCCCCCG AAGATCATCA TCAGGAACCA AGGTCCATCG 
AAATCCAGCT TGCAGCAGGA TCTCAAAGAC TTCTCCCAGA AGGTGGCTCA GGTGTACCTG 
GCTATAGATG ACCGTCTTAC CTCCTTAAAA ACGGACACAT TCAGCAAGAC CAGAGAGGAA 
AAAATGGAGG ACATGTTTGC ACAGAAAGAT ATGGAGGAAT CAGAGCTTCG GGGCTGGATA 
GAGAAGTTAC AAGTGCGTCT GCAGACGAGT GTGATTGACT CTTCACAACA ACTACAGGCT 
GTTCTGGAGT CAGTGGTGGT CAAAAAACAG GGCTTGTGTG AAACCCTGCA GTCCTGGAAC 
AACAGACTTC AAGAACTGTT CCAGCAAGAG AAAGGCAGAA AACGTCTATC TGTTCCTCCT 
AGCCCAGGCA GACACAGACA ACCTACAACA GATGAGAGCA AGACTAGTGC TTTGGAGTCT 
TCTCCTCGTA ACCCTTCCCC AGTGGTCCCA AATGGAGACA AAGAGGACCG TCATCTCAAT 
ACATTTCCAT CAAGTTCAGG GTCCTCGTCG TTACTACAGT TACCATCTCC AGCTGAACAA 
CCTTCAGATG TCATTACAAG CGGACCATCT TTCCCTGATC AGGACTCTGT TAGCAATCCA 
GAGGACATGT TTGATGGACA CTTGCTTGGT TCCAATGATA GTCAGGTAAA GGAAAAGTCC 
ACCATGAAAA CAATCCTGGC CAACCTGTTA CCTGGAAACA GCTACAATCC CATCCCTTTT 
CCTTTTGACC CAGACAAGCA CTATCTGATG TATGAGCATG AGAGAGTTCC TATAGCCGTG 
TGTGAGAGAG AGCCCAGCTC CATAATTGCC TTTGCACTCA GCTGCAAAGA GTATAAAACT 
GCCCTTGAAG AGCTCACAAA GACAACAGCA AAGACTGGAG GTGATGACAC TTCTCAGACC 
ATCAGTGCTG GAGAGAGCAG AGTGAAGAAC AGTCCAGCCA AACCCAGTGA CAACAGCATG 
TCACAGCTGA GCCGCAGCAG CGTCGACGCT GATCCACTCA AGGAGCCTGA AAGTGCAGAC 
AAACAGAAGA AGCAGACAGG GAACCCACAT ATTGAGCTGC AGTTCTCCGA TGCAAATGCT 
AAGTTCTACT GCCGGATCTA CTATGCTGAA GAGTTCCATA AAATGCGGGA GGAGATCATG 
GAGAGCACAG AGGATGATTT TGTGCGATCT CTTTCCCACT GTGTCAACTG GCAGGCGCGT 
GGTGGGAAAT CTGGTGCAGT TTTCTATGCC ACTGAAGATG ATCGGTTTAT TCTGAAGCAG 
ATGCCCAGAT TAGAGGTCCA GTCCTTTTTA GACTTTGCAC CCCACTACTT TACGTACATC 
ACAGGAGCGG TTCACCTAAA GCGACCCACA GCACTTGCTA AGATTCTGGG AGTGTACCGC 
ATCGGCTACA AGAACTCCCA GAACAACACT GAGAAGAAGC TTGACCTGCT TGTGATGGAA 
AACCTTTTCT ATGGACGAAA GATGGCACAG GTGTTTGACC TGAAAGGATC GCTGAGAAAC 
AGGAACGTGA AGACGGACCA GGGAAAGGAG AGCTGTGAGG TGGTGCTCCT AGATGAGAAC 
CTGCTCAAAC TGGTGCATGA CAATCCCCTT TATATTCGCT CGCACTGCAA GGCCATCCTG 
CGTGCTGCCA TCCACAGCGA CGCGCTCTTC CTGTCCAGCC ACCTCATCAT TGATTACTCC 
CTGCTGGTGG GCCGAGACGA TTCCACAGAT GAGCTAGTTG TGGGGATCAT AGATTATATC 
CGGACTTTCA CTTGGGATAA AAAGCTGGAG ATGGTGGTCA AATCTACTGG GATTCTTGGA 
GGTCAAGGTA AAATGCCCAC AGTGGTGTCA CCAGAGCTGT ATCGAACACG TTTCTGTGAG 
GCCATGGATA AATACTTCCT TATGGTCCCT GATCACTGGA GTGGACTTGG GCTCAACTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167723.1
CDS	164..6466
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167666.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167666.4

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGAA AAAATTCCAT TGGGATGAGG 
AAGAATCATC AGGAATCTCA GAACAGCGGT CTCAATTCCA GACTGACAGT GGTCCAAGAG 
GATATTGGAA AGTCACCTGC CAGGAAGAGG TCAGCTAGTG TGACCAATTT GTCTTTGGAC 
CGTTCCGGCT CATCCATGGT TCCTGCCTAT GAGAGTTCGG TTAGTCCACA GAACAGCCGA 
GCTCTACCCA AGACCGACCA CAGCGAAGAA GAGCGAAAGA TCCTTCTAGA TTCTTTTCAA 
CTTAAAGATA TATGGAAGAA GATTTGCCAC AACACTACTG GGATGGAGTT CCAGGACCAT 
CGGTACTGGC TGCGCACTTA CCCCAACTGC ATTGTGGGTA AAGAGTTGGT CAACTGGCTT 
TTAAGAAATG GCACTATTTC CACTAGGGCT CAAGCCATAG CTATAGGACA GGCTTTGGTA 
GATGGCCGCT GGCTTGACTG TGTAACCCAT CATGATCAGA TCTTCCGTGA TGAGTACGCC 
CTTTATCGCC CCCTTCAGAG CACAGAGTTC TCCGAAACTC CTTCTCCAGA CAGTGACAGT 
GTTAATTCCC TGGAGGGACA CTCTGAACCG TCATGGTTTA AAGACATCAA GTTTGATGAC 
AGTGACACCG AGCAGCTAGC AGATGAAAAT GACTATGTCA CACCAAGCTC GTCCAGCCCC 
AGCAAAAGAA CATCTGTCAG CAGTTTCCAC TCTGCAGTAG ACAGTGACTC GGCCGCCTCC 
ATCAGTCTAA ACATGGAGCA GGACAACGTC AATTTCCACA TTAAGAAGCA GGCCAAGTAC 
CCCCATGTGC CTCCCTACCC AGCTGAGCAA AAAATGGAAG TCTTGCTCTC CGAAGATGGA 
GGTCAGCATA TATCCATCAG TGATGCATTC ATCAAAGAGT CTTTGTTCAA CCGGAGGGTT 
GAGGAGAAAG CTAAAGAGAT GCTCTTCACT CCTCTCGGCT GGCATCACAG CTCTCTGGAT 
CAGCTGCGGG AAGAGAATGG GGAAAAGAAA GCGATGGAGC GGTTGCTGTC AGCAAATCAC 
AGCCACATGA TGGCGCTGCT GCAGCAACTG CTGTACAGTG AATCACTGTC TCTGTCGTGG 
CGTGACATCA TCGTACCTGT GGTGAGGCAG GTGGTGCAGA CGGTGCGACC GGATGTGCGC 
AGCTGTGACG ATGATATGGA CATCCGTCAG TTTGTCCACA TCAAGAAGAT TCCAGGAGGA 
AAGAAGTTTG ACTCTGCTGT AGTGAATGGT TTTGTTTGCA CAAAGAATAT CGCACACAAA 
AAGATGAACT CTTACATCAA AAACCCCAAG ATCCTTCTTC TCAAATGCTC CATTGAGTAT 
CTATATAGAG AGGAGACCAA GTTCACCTGC ATTGACCCAA TTGTTTTACA GGAGCGTGAG 
TTTCTTAAGA ACTATGTTCA GAGGATTGTT GATGTCCGAC CAAACTTAGT CCTGGTGGAG 
AAGACTGTAT CGCGAATTGC TCAGGACATG CTACTAGAGC ACGGCATTGC CATTGTGATT 
AATGTCAAAC CTCAAGTACT GGACCGTGTG AGTCGAATGA CTCAGGGAGA TTTAGTCATT 
TCAATGGATC AGCTTCTTAC AAAACCTCGT CTGGGTACCT GCCACAAGTT TTACTTGCAC 
TCCTTCCAGC TACCAAATAG TGAAGTGAAA ACCCTGATGT TTTTTGAGGG CTGTCCTCCT 
CAGCTGGGCT GCACTATTAA GCTCCGCGGA GCCTCAGAGT ATGAGCTGGC ACGGGTAAAA 
GAGATCATCA TTTTTATGGT GTGTGTGGCA TACCACTCGC AGCTAGAGAT CTCTTTCCTC 
ATGGATGAGT TCGCTATGCC TCCCAGTCTG GCTGAGAGTT CTTCTTTCCC ATGTCTGCTG 
GAAAGCACCA CTTTACGGGA AGAGGATGAA ACTAGTGGGG AACGGGAAAG CCAAGACAAT 
GATGAGTCTA CACTGGGAGA TGACAATCTC ACACTTGTTC CAGGAGATGA CTTTGAGACA 
GGATTTACAG GGACTATCAA AGCCCACAGT CGAGAGCCTT CCACCTCTGA ATCTTCTCAT 
AAAGATGGAG AAAGCCCCAG AATAAATAAA AATGCTTCAA TTACCTCCTT CTCTGGAGGG 
GAGGAAGAAA ATATAAAGAC CTCCACGCCA CTTTCCTCCT TCTCTTCCAC CTTTCCTCAG 
CCGGTGACAC CACCTTTCCT GATATCAGAC GTAAAAGAAA TGTCACAGGA AGTGGTCAAG 
GGGTCAGGTG AAGGGGAGAA GAACAAATAT CTGGAGTCGA CTCTGGTCCA TCAGGACAGC 
ACAAGCTCTG AGACCTCCCT TCCCCCAACT CGGCTCTTTA GAGACCCCTT ACAGGACGAC 
ACAGGCCTGT TTGTGACAGA GCATGTAGCT TCTTCAGATG ACCGCCTCAA ATCCATCTCT 
GCTTTATTCA AACAGGAGCT AAAAGACATC ATCCTGTGCA TTTCACCCTT TATTACCTTT 
CGAGAGCCTT TCTTGCTTAC CCCAGCTGGA CTGCGGTGTC CTAGCCGGGA TTATTTCCCT 
GAACAGGTTT ACCTTTCACC TCTGCTGAAC AAGGATTCAA AAGAACTGGA TGGACGCCGC 
AAGAGGCAGC TGCTGAAAGA GTCCAGCCCA GGTTCTGGTA GCCTGACCAA TGGTTCCGTT 
TCACACCAGA GAGCCATTCA GATCTTGCCC TGTCACAAAC TCACTAGTGC CCGAATAGCA 
GAACAGGTAG GCAGCAGTCA GCAGCTGGCA CGCATGTTAG CGGACTACCG TGCCCAAGGA 
GGACGTATCC GGCAAAGAGA GGGAACACAA TTTCGTGAAG CCCCTCCCCC AAAGGCGCCA 
ATAAAGGCAG ATAGTGAAGA AGATAAAGGT GCAGGAGTGA TTGACATGAC CTGGTCTACT 
AAGTTGGACT GTTTAAATCC AATCAACCAT CAGAGGCTCT GTGTTCTGTT CAGTAGCTCT 
TCAGCACAGT CTAATAATGC CCCAAACCCC TGCGTCAGTC CCTGGATTGT TACAATGGAG 
TTTTATGGAA AGAATGACTT GACTCTTGGT GTGTTTCTGG AGAGATACTG TTTTAGACCA 
TCTTACCAGT GCCCCAGCAT GTACTGTGAG ACCCCCATGG TTCACCACAT CCGGCGCTTT 
GTGCACGGTA AAGGCTGCGT CCAGATTGTA CTGAAAGAGC TGGACTCGCC TGTTCCTGGA 
TATCAGCACA CAATCCTCAA CTACTCATGG TGTCGCATCT GCAAACAGGT GACTCCTGTG 
GTCCCTTTGT CTAATGACTC TTGGTCCATG TCCTTCGCCA AGTACCTTGA GCTTCGTTTC 
TATGGTCATC AGTACACCCG TCGGGCTAAC GCTGAGCCTT GTGGCCACTC TATCCATAAA 
GATTATCACC AGTACTTCTC TTACAACCAG ATGGTGGCAT CCTTCAGCTA CATTCCAGTG 
AGGCTGTTAG AGATCTGTCT GCCTCCCCCG AAGATCATCA TCAGGAACCA AGGTCCATCG 
AAATCCAGCT TGCAGCAGGA TCTCAAAGAC TTCTCCCAGA AGGTGGCTCA GGTGTACCTG 
GCTATAGATG ACCGTCTTAC CTCCTTAAAA ACGGACACAT TCAGCAAGAC CAGAGAGGAA 
AAAATGGAGG ACATGTTTGC ACAGAAAGAT ATGGAGGAAT CAGAGCTTCG GGGCTGGATA 
GAGAAGTTAC AAGTGCGTCT GCAGACGAGT GTGATTGACT CTTCACAACA ACTACAGGCT 
GTTCTGGAGT CAGTGGTGGT CAAAAAACAG GGCTTGTGTG AAACCCTGCA GTCCTGGAAC 
AACAGACTTC AAGAACTGTT CCAGCAAGAG AAAGGCAGAA AACGTCTATC TGTTCCTCCT 
AGCCCAGGCA GACACAGACA ACCTACAACA GATGAGAGCA AGACTAGTGC TTTGGAGTCT 
TCTCCTCGTA ACCCTTCCCC AGTGGTCCCA AATGGAGACA AAGAGGACCG TCATCTCAAT 
ACATTTCCAT CAAGTTCAGG GTCCTCGTCG TTACTACAGT TACCATCTCC AGCTGAACAA 
CCTTCAGATG TCATTACAAG CGGACCATCT TTCCCTGATC AGGACTCTGT TAGCAATCCA 
GAGGACATGT TTGATGGACA CTTGCTTGGT TCCAATGATA GTCAGGTAAA GGAAAAGTCC 
ACCATGAAAA CAATCCTGGC CAACCTGTTA CCTGGAAACA GCTACAATCC CATCCCTTTT 
CCTTTTGACC CAGACAAGCA CTATCTGATG TATGAGCATG AGAGAGTTCC TATAGCCGTG 
TGTGAGAGAG AGCCCAGCTC CATAATTGCC TTTGCACTCA GCTGCAAAGA GTATAAAACT 
GCCCTTGAAG AGCTCACAAA GACAACAGCA AAGACTGGAG GTGATGACAC TTCTCAGACC 
ATCAGTGCTG GAGAGAGCAG AGTGAAGAAC AGTCCAGCCA AACCCAGTGA CAACAGCATG 
TCACAGCTGA GCCGCAGCAG CGTCGACGCT GATCCACTCA AGGAGCCTGA AAGTGCAGAC 
AAACAGAAGA AGCAGACAGG GAACCCACAT ATTGAGCTGC AGTTCTCCGA TGCAAATGCT 
AAGTTCTACT GCCGGATCTA CTATGCTGAA GAGTTCCATA AAATGCGGGA GGAGATCATG 
GAGAGCACAG AGGATGATTT TGTGCGATCT CTTTCCCACT GTGTCAACTG GCAGGCGCGT 
GGTGGGAAAT CTGGTGCAGT TTTCTATGCC ACTGAAGATG ATCGGTTTAT TCTGAAGCAG 
ATGCCCAGAT TAGAGGTCCA GTCCTTTTTA GACTTTGCAC CCCACTACTT TACGTACATC 
ACAGGAGCGG TTCACCTAAA GCGACCCACA GCACTTGCTA AGATTCTGGG AGTGTACCGC 
ATCGGCTACA AGAACTCCCA GAACAACACT GAGAAGAAGC TTGACCTGCT TGTGATGGAA 
AACCTTTTCT ATGGACGAAA GATGGCACAG GTGTTTGACC TGAAAGGATC GCTGAGAAAC 
AGGAACGTGA AGACGGACCA GGGAAAGGAG AGCTGTGAGG TGGTGCTCCT AGATGAGAAC 
CTGCTCAAAC TGGTGCATGA CAATCCCCTT TATATTCGCT CGCACTGCAA GGCCATCCTG 
CGTGCTGCCA TCCACAGCGA CGCGCTCTTC CTGTCCAGCC ACCTCATCAT TGATTACTCC 
CTGCTGGTGG GCCGAGACGA TTCCACAGAT GAGCTAGTTG TGGGGATCAT AGATTATATC 
CGGACTTTCA CTTGGGATAA AAAGCTGGAG ATGGTGGTCA AATCTACTGG GATTCTTGGA 
GGTCAAGGTA AAATGCCCAC AGTGGTGTCA CCAGAGCTGT ATCGAACACG TTTCTGTGAG 
GCCATGGATA AATACTTCCT TATGGTCCCT GATCACTGGA GTGGACTTGG GCTCAACTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167667.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167724.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.

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CloneID	ODa15217
Clone ID Related Accession (Same CDS sequence)	XM_005167667.4 , XM_005167667.3
Accession Version	XM_005167667.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167667.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGTC ATCAGGAATC TCAGAACAGC 
GGTCTCAATT CCAGACTGAC AGTGGTCCAA GAGGATATTG GAAAGTCACC TGCCAGGAAG 
AGGTCAGCTA GTGTGACCAA TTTGTCTTTG GACCGTTCCG GCTCATCCAT GGTTCCTGCC 
TATGAGAGTT CGGTTAGTCC ACAGAACAGC CGAGCTCTAC CCAAGACCGA CCACAGCGAA 
GAAGAGCGAA AGATCCTTCT AGATTCTTTT CAACTTAAAG ATATATGGAA GAAGATTTGC 
CACAACACTA CTGGGATGGA GTTCCAGGAC CATCGGTACT GGCTGCGCAC TTACCCCAAC 
TGCATTGTGG GTAAAGAGTT GGTCAACTGG CTTTTAAGAA ATGGCACTAT TTCCACTAGG 
GCTCAAGCCA TAGCTATAGG ACAGGCTTTG GTAGATGGCC GCTGGCTTGA CTGTGTAACC 
CATCATGATC AGATCTTCCG TGATGAGTAC GCCCTTTATC GCCCCCTTCA GAGCACAGAG 
TTCTCCGAAA CTCCTTCTCC AGACAGTGAC AGTGTTAATT CCCTGGAGGG ACACTCTGAA 
CCGTCATGGT TTAAAGACAT CAAGTTTGAT GACAGTGACA CCGAGCAGCT AGCAGATGAA 
AATGACTATG TCACACCAAG CTCGTCCAGC CCCAGCAAAA GAACATCTGT CAGCAGTTTC 
CACTCTGCAG TAGACAGTGA CTCGGCCGCC TCCATCAGTC TAAACATGGA GCAGGACAAC 
GTCAATTTCC ACATTAAGAA GCAGGCCAAG TACCCCCATG TGCCTCCCTA CCCAGCTGAG 
CAAAAAATGG AAGTCTTGCT CTCCGAAGAT GGAGGTCAGC ATATATCCAT CAGTGATGCA 
TTCATCAAAG AGTCTTTGTT CAACCGGAGG GTTGAGGAGA AAGCTAAAGA GATGCTCTTC 
ACTCCTCTCG GCTGGCATCA CAGCTCTCTG GATCAGCTGC GGGAAGAGAA TGGGGAAAAG 
AAAGCGATGG AGCGGTTGCT GTCAGCAAAT CACAGCCACA TGATGGCGCT GCTGCAGCAA 
CTGCTGTACA GTGAATCACT GTCTCTGTCG TGGCGTGACA TCATCGTACC TGTGGTGAGG 
CAGGTGGTGC AGACGGTGCG ACCGGATGTG CGCAGCTGTG ACGATGATAT GGACATCCGT 
CAGTTTGTCC ACATCAAGAA GATTCCAGGA GGAAAGAAGT TTGACTCTGC TGTAGTGAAT 
GGTTTTGTTT GCACAAAGAA TATCGCACAC AAAAAGATGA ACTCTTACAT CAAAAACCCC 
AAGATCCTTC TTCTCAAATG CTCCATTGAG TATCTATATA GAGAGGAGAC CAAGTTCACC 
TGCATTGACC CAATTGTTTT ACAGGAGCGT GAGTTTCTTA AGAACTATGT TCAGAGGATT 
GTTGATGTCC GACCAAACTT AGTCCTGGTG GAGAAGACTG TATCGCGAAT TGCTCAGGAC 
ATGCTACTAG AGCACGGCAT TGCCATTGTG ATTAATGTCA AACCTCAAGT ACTGGACCGT 
GTGAGTCGAA TGACTCAGGG AGATTTAGTC ATTTCAATGG ATCAGCTTCT TACAAAACCT 
CGTCTGGGTA CCTGCCACAA GTTTTACTTG CACTCCTTCC AGCTACCAAA TAGTGAAGTG 
AAAACCCTGA TGTTTTTTGA GGGCTGTCCT CCTCAGCTGG GCTGCACTAT TAAGCTCCGC 
GGAGCCTCAG AGTATGAGCT GGCACGGGTA AAAGAGATCA TCATTTTTAT GGTGTGTGTG 
GCATACCACT CGCAGCTAGA GATCTCTTTC CTCATGGATG AGTTCGCTAT GCCTCCCAGT 
CTGGCTGAGA GTTCTTCTTT CCCATGTCTG CTGGAAAGCA CCACTTTACG GGAAGAGGAT 
GAAACTAGTG GGGAACGGGA AAGCCAAGAC AATGATGAGT CTACACTGGG AGATGACAAT 
CTCACACTTG TTCCAGGAGA TGACTTTGAG ACAGGATTTA CAGGGACTAT CAAAGCCCAC 
AGTCGAGAGC CTTCCACCTC TGAATCTTCT CATAAAGATG GAGAAAGCCC CAGAATAAAT 
AAAAATGCTT CAATTACCTC CTTCTCTGGA GGGGAGGAAG AAAATATAAA GACCTCCACG 
CCACTTTCCT CCTTCTCTTC CACCTTTCCT CAGCCGGTGA CACCACCTTT CCTGATATCA 
GACGTAAAAG AAATGTCACA GGAAGTGGTC AAGGGGTCAG GTGAAGGGGA GAAGAACAAA 
TATCTGGAGT CGACTCTGGT CCATCAGGAC AGCACAAGCT CTGAGACCTC CCTTCCCCCA 
ACTCGGCTCT TTAGAGACCC CTTACAGGAC GACACAGGCC TGTTTGTGAC AGAGCATGTA 
GCTTCTTCAG ATGACCGCCT CAAATCCATC TCTGCTTTAT TCAAACAGGA GCTAAAAGAC 
ATCATCCTGT GCATTTCACC CTTTATTACC TTTCGAGAGC CTTTCTTGCT TACCCCAGCT 
GGACTGCGGT GTCCTAGCCG GGATTATTTC CCTGAACAGG TTTACCTTTC ACCTCTGCTG 
AACAAGGATT CAAAAGAACT GGATGGACGC CGCAAGAGGC AGCTGCTGAA AGAGTCCAGC 
CCAGGTTCTG GTAGCCTGAC CAATGGTTCC GTTTCACACC AGAGAGCCAT TCAGATCTTG 
CCCTGTCACA AACTCACTAG TGCCCGAATA GCAGAACAGG TAGGCAGCAG TCAGCAGCTG 
GCACGCATGT TAGCGGACTA CCGTGCCCAA GGAGGACGTA TCCGGCAAAG AGAGGGAACA 
CAATTTCGTG AAGCCCCTCC CCCAAAGGCG CCAATAAAGG CAGATAGTGA AGAAGATAAA 
GGTGCAGGAG TGATTGACAT GACCTGGTCT ACTAAGTTGG ACTGTTTAAA TCCAATCAAC 
CATCAGAGGC TCTGTGTTCT GTTCAGTAGC TCTTCAGCAC AGTCTAATAA TGCCCCAAAC 
CCCTGCGTCA GTCCCTGGAT TGTTACAATG GAGTTTTATG GAAAGAATGA CTTGACTCTT 
GGTGTGTTTC TGGAGAGATA CTGTTTTAGA CCATCTTACC AGTGCCCCAG CATGTACTGT 
GAGACCCCCA TGGTTCACCA CATCCGGCGC TTTGTGCACG GTAAAGGCTG CGTCCAGATT 
GTACTGAAAG AGCTGGACTC GCCTGTTCCT GGATATCAGC ACACAATCCT CAACTACTCA 
TGGTGTCGCA TCTGCAAACA GGTGACTCCT GTGGTCCCTT TGTCTAATGA CTCTTGGTCC 
ATGTCCTTCG CCAAGTACCT TGAGCTTCGT TTCTATGGTC ATCAGTACAC CCGTCGGGCT 
AACGCTGAGC CTTGTGGCCA CTCTATCCAT AAAGATTATC ACCAGTACTT CTCTTACAAC 
CAGATGGTGG CATCCTTCAG CTACATTCCA GTGAGGCTGT TAGAGATCTG TCTGCCTCCC 
CCGAAGATCA TCATCAGGAA CCAAGGTCCA TCGAAATCCA GCTTGCAGCA GGATCTCAAA 
GACTTCTCCC AGAAGGTGGC TCAGGTGTAC CTGGCTATAG ATGACCGTCT TACCTCCTTA 
AAAACGGACA CATTCAGCAA GACCAGAGAG GAAAAAATGG AGGACATGTT TGCACAGAAA 
GATATGGAGG AATCAGAGCT TCGGGGCTGG ATAGAGAAGT TACAAGTGCG TCTGCAGACG 
AGTGTGATTG ACTCTTCACA ACAACTACAG GCTGTTCTGG AGTCAGTGGT GGTCAAAAAA 
CAGGGCTTGT GTGAAACCCT GCAGTCCTGG AACAACAGAC TTCAAGAACT GTTCCAGCAA 
GAGAAAGGCA GAAAACGTCT ATCTGTTCCT CCTAGCCCAG GCAGACACAG ACAACCTACA 
ACAGATGAGA GCAAGACTAG TGCTTTGGAG TCTTCTCCTC GTAACCCTTC CCCAGTGGTC 
CCAAATGGAG ACAAAGAGGA CCGTCATCTC AATACATTTC CATCAAGTTC AGGGTCCTCG 
TCGTTACTAC AGTTACCATC TCCAGCTGAA CAACCTTCAG ATGTCATTAC AAGCGGACCA 
TCTTTCCCTG ATCAGGACTC TGTTAGCAAT CCAGAGGACA TGTTTGATGG ACACTTGCTT 
GGTTCCAATG ATAGTCAGGT AAAGGAAAAG TCCACCATGA AAACAATCCT GGCCAACCTG 
TTACCTGGAA ACAGCTACAA TCCCATCCCT TTTCCTTTTG ACCCAGACAA GCACTATCTG 
ATGTATGAGC ATGAGAGAGT TCCTATAGCC GTGTGTGAGA GAGAGCCCAG CTCCATAATT 
GCCTTTGCAC TCAGCTGCAA AGAGTATAAA ACTGCCCTTG AAGAGCTCAC AAAGACAACA 
GCAAAGACTG GAGGTGATGA CACTTCTCAG ACCATCAGTG CTGGAGAGAG CAGAGTGAAG 
AACAGTCCAG CCAAACCCAG TGACAACAGC ATGTCACAGC TGAGCCGCAG CAGCGTCGAC 
GCTGATCCAC TCAAGGAGCC TGAAAGTGCA GACAAACAGA AGAAGCAGAC AGGGAACCCA 
CATATTGAGC TGCAGTTCTC CGATGCAAAT GCTAAGTTCT ACTGCCGGAT CTACTATGCT 
GAAGAGTTCC ATAAAATGCG GGAGGAGATC ATGGAGAGCA CAGAGGATGA TTTTGTGCGA 
TCTCTTTCCC ACTGTGTCAA CTGGCAGGCG CGTGGTGGGA AATCTGGTGC AGTTTTCTAT 
GCCACTGAAG ATGATCGGTT TATTCTGAAG CAGATGCCCA GATTAGAGGT CCAGTCCTTT 
TTAGACTTTG CACCCCACTA CTTTACGTAC ATCACAGGAG CGGTTCACCT AAAGCGACCC 
ACAGCACTTG CTAAGATTCT GGGAGTGTAC CGCATCGGCT ACAAGAACTC CCAGAACAAC 
ACTGAGAAGA AGCTTGACCT GCTTGTGATG GAAAACCTTT TCTATGGACG AAAGATGGCA 
CAGGTGTTTG ACCTGAAAGG ATCGCTGAGA AACAGGAACG TGAAGACGGA CCAGGGAAAG 
GAGAGCTGTG AGGTGGTGCT CCTAGATGAG AACCTGCTCA AACTGGTGCA TGACAATCCC 
CTTTATATTC GCTCGCACTG CAAGGCCATC CTGCGTGCTG CCATCCACAG CGACGCGCTC 
TTCCTGTCCA GCCACCTCAT CATTGATTAC TCCCTGCTGG TGGGCCGAGA CGATTCCACA 
GATGAGCTAG TTGTGGGGAT CATAGATTAT ATCCGGACTT TCACTTGGGA TAAAAAGCTG 
GAGATGGTGG TCAAATCTAC TGGGATTCTT GGAGGTCAAG GTAAAATGCC CACAGTGGTG 
TCACCAGAGC TGTATCGAAC ACGTTTCTGT GAGGCCATGG ATAAATACTT CCTTATGGTC 
CCTGATCACT GGAGTGGACT TGGGCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167724.1
CDS	164..6439
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQSHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167667.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167667.4

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGTC ATCAGGAATC TCAGAACAGC 
GGTCTCAATT CCAGACTGAC AGTGGTCCAA GAGGATATTG GAAAGTCACC TGCCAGGAAG 
AGGTCAGCTA GTGTGACCAA TTTGTCTTTG GACCGTTCCG GCTCATCCAT GGTTCCTGCC 
TATGAGAGTT CGGTTAGTCC ACAGAACAGC CGAGCTCTAC CCAAGACCGA CCACAGCGAA 
GAAGAGCGAA AGATCCTTCT AGATTCTTTT CAACTTAAAG ATATATGGAA GAAGATTTGC 
CACAACACTA CTGGGATGGA GTTCCAGGAC CATCGGTACT GGCTGCGCAC TTACCCCAAC 
TGCATTGTGG GTAAAGAGTT GGTCAACTGG CTTTTAAGAA ATGGCACTAT TTCCACTAGG 
GCTCAAGCCA TAGCTATAGG ACAGGCTTTG GTAGATGGCC GCTGGCTTGA CTGTGTAACC 
CATCATGATC AGATCTTCCG TGATGAGTAC GCCCTTTATC GCCCCCTTCA GAGCACAGAG 
TTCTCCGAAA CTCCTTCTCC AGACAGTGAC AGTGTTAATT CCCTGGAGGG ACACTCTGAA 
CCGTCATGGT TTAAAGACAT CAAGTTTGAT GACAGTGACA CCGAGCAGCT AGCAGATGAA 
AATGACTATG TCACACCAAG CTCGTCCAGC CCCAGCAAAA GAACATCTGT CAGCAGTTTC 
CACTCTGCAG TAGACAGTGA CTCGGCCGCC TCCATCAGTC TAAACATGGA GCAGGACAAC 
GTCAATTTCC ACATTAAGAA GCAGGCCAAG TACCCCCATG TGCCTCCCTA CCCAGCTGAG 
CAAAAAATGG AAGTCTTGCT CTCCGAAGAT GGAGGTCAGC ATATATCCAT CAGTGATGCA 
TTCATCAAAG AGTCTTTGTT CAACCGGAGG GTTGAGGAGA AAGCTAAAGA GATGCTCTTC 
ACTCCTCTCG GCTGGCATCA CAGCTCTCTG GATCAGCTGC GGGAAGAGAA TGGGGAAAAG 
AAAGCGATGG AGCGGTTGCT GTCAGCAAAT CACAGCCACA TGATGGCGCT GCTGCAGCAA 
CTGCTGTACA GTGAATCACT GTCTCTGTCG TGGCGTGACA TCATCGTACC TGTGGTGAGG 
CAGGTGGTGC AGACGGTGCG ACCGGATGTG CGCAGCTGTG ACGATGATAT GGACATCCGT 
CAGTTTGTCC ACATCAAGAA GATTCCAGGA GGAAAGAAGT TTGACTCTGC TGTAGTGAAT 
GGTTTTGTTT GCACAAAGAA TATCGCACAC AAAAAGATGA ACTCTTACAT CAAAAACCCC 
AAGATCCTTC TTCTCAAATG CTCCATTGAG TATCTATATA GAGAGGAGAC CAAGTTCACC 
TGCATTGACC CAATTGTTTT ACAGGAGCGT GAGTTTCTTA AGAACTATGT TCAGAGGATT 
GTTGATGTCC GACCAAACTT AGTCCTGGTG GAGAAGACTG TATCGCGAAT TGCTCAGGAC 
ATGCTACTAG AGCACGGCAT TGCCATTGTG ATTAATGTCA AACCTCAAGT ACTGGACCGT 
GTGAGTCGAA TGACTCAGGG AGATTTAGTC ATTTCAATGG ATCAGCTTCT TACAAAACCT 
CGTCTGGGTA CCTGCCACAA GTTTTACTTG CACTCCTTCC AGCTACCAAA TAGTGAAGTG 
AAAACCCTGA TGTTTTTTGA GGGCTGTCCT CCTCAGCTGG GCTGCACTAT TAAGCTCCGC 
GGAGCCTCAG AGTATGAGCT GGCACGGGTA AAAGAGATCA TCATTTTTAT GGTGTGTGTG 
GCATACCACT CGCAGCTAGA GATCTCTTTC CTCATGGATG AGTTCGCTAT GCCTCCCAGT 
CTGGCTGAGA GTTCTTCTTT CCCATGTCTG CTGGAAAGCA CCACTTTACG GGAAGAGGAT 
GAAACTAGTG GGGAACGGGA AAGCCAAGAC AATGATGAGT CTACACTGGG AGATGACAAT 
CTCACACTTG TTCCAGGAGA TGACTTTGAG ACAGGATTTA CAGGGACTAT CAAAGCCCAC 
AGTCGAGAGC CTTCCACCTC TGAATCTTCT CATAAAGATG GAGAAAGCCC CAGAATAAAT 
AAAAATGCTT CAATTACCTC CTTCTCTGGA GGGGAGGAAG AAAATATAAA GACCTCCACG 
CCACTTTCCT CCTTCTCTTC CACCTTTCCT CAGCCGGTGA CACCACCTTT CCTGATATCA 
GACGTAAAAG AAATGTCACA GGAAGTGGTC AAGGGGTCAG GTGAAGGGGA GAAGAACAAA 
TATCTGGAGT CGACTCTGGT CCATCAGGAC AGCACAAGCT CTGAGACCTC CCTTCCCCCA 
ACTCGGCTCT TTAGAGACCC CTTACAGGAC GACACAGGCC TGTTTGTGAC AGAGCATGTA 
GCTTCTTCAG ATGACCGCCT CAAATCCATC TCTGCTTTAT TCAAACAGGA GCTAAAAGAC 
ATCATCCTGT GCATTTCACC CTTTATTACC TTTCGAGAGC CTTTCTTGCT TACCCCAGCT 
GGACTGCGGT GTCCTAGCCG GGATTATTTC CCTGAACAGG TTTACCTTTC ACCTCTGCTG 
AACAAGGATT CAAAAGAACT GGATGGACGC CGCAAGAGGC AGCTGCTGAA AGAGTCCAGC 
CCAGGTTCTG GTAGCCTGAC CAATGGTTCC GTTTCACACC AGAGAGCCAT TCAGATCTTG 
CCCTGTCACA AACTCACTAG TGCCCGAATA GCAGAACAGG TAGGCAGCAG TCAGCAGCTG 
GCACGCATGT TAGCGGACTA CCGTGCCCAA GGAGGACGTA TCCGGCAAAG AGAGGGAACA 
CAATTTCGTG AAGCCCCTCC CCCAAAGGCG CCAATAAAGG CAGATAGTGA AGAAGATAAA 
GGTGCAGGAG TGATTGACAT GACCTGGTCT ACTAAGTTGG ACTGTTTAAA TCCAATCAAC 
CATCAGAGGC TCTGTGTTCT GTTCAGTAGC TCTTCAGCAC AGTCTAATAA TGCCCCAAAC 
CCCTGCGTCA GTCCCTGGAT TGTTACAATG GAGTTTTATG GAAAGAATGA CTTGACTCTT 
GGTGTGTTTC TGGAGAGATA CTGTTTTAGA CCATCTTACC AGTGCCCCAG CATGTACTGT 
GAGACCCCCA TGGTTCACCA CATCCGGCGC TTTGTGCACG GTAAAGGCTG CGTCCAGATT 
GTACTGAAAG AGCTGGACTC GCCTGTTCCT GGATATCAGC ACACAATCCT CAACTACTCA 
TGGTGTCGCA TCTGCAAACA GGTGACTCCT GTGGTCCCTT TGTCTAATGA CTCTTGGTCC 
ATGTCCTTCG CCAAGTACCT TGAGCTTCGT TTCTATGGTC ATCAGTACAC CCGTCGGGCT 
AACGCTGAGC CTTGTGGCCA CTCTATCCAT AAAGATTATC ACCAGTACTT CTCTTACAAC 
CAGATGGTGG CATCCTTCAG CTACATTCCA GTGAGGCTGT TAGAGATCTG TCTGCCTCCC 
CCGAAGATCA TCATCAGGAA CCAAGGTCCA TCGAAATCCA GCTTGCAGCA GGATCTCAAA 
GACTTCTCCC AGAAGGTGGC TCAGGTGTAC CTGGCTATAG ATGACCGTCT TACCTCCTTA 
AAAACGGACA CATTCAGCAA GACCAGAGAG GAAAAAATGG AGGACATGTT TGCACAGAAA 
GATATGGAGG AATCAGAGCT TCGGGGCTGG ATAGAGAAGT TACAAGTGCG TCTGCAGACG 
AGTGTGATTG ACTCTTCACA ACAACTACAG GCTGTTCTGG AGTCAGTGGT GGTCAAAAAA 
CAGGGCTTGT GTGAAACCCT GCAGTCCTGG AACAACAGAC TTCAAGAACT GTTCCAGCAA 
GAGAAAGGCA GAAAACGTCT ATCTGTTCCT CCTAGCCCAG GCAGACACAG ACAACCTACA 
ACAGATGAGA GCAAGACTAG TGCTTTGGAG TCTTCTCCTC GTAACCCTTC CCCAGTGGTC 
CCAAATGGAG ACAAAGAGGA CCGTCATCTC AATACATTTC CATCAAGTTC AGGGTCCTCG 
TCGTTACTAC AGTTACCATC TCCAGCTGAA CAACCTTCAG ATGTCATTAC AAGCGGACCA 
TCTTTCCCTG ATCAGGACTC TGTTAGCAAT CCAGAGGACA TGTTTGATGG ACACTTGCTT 
GGTTCCAATG ATAGTCAGGT AAAGGAAAAG TCCACCATGA AAACAATCCT GGCCAACCTG 
TTACCTGGAA ACAGCTACAA TCCCATCCCT TTTCCTTTTG ACCCAGACAA GCACTATCTG 
ATGTATGAGC ATGAGAGAGT TCCTATAGCC GTGTGTGAGA GAGAGCCCAG CTCCATAATT 
GCCTTTGCAC TCAGCTGCAA AGAGTATAAA ACTGCCCTTG AAGAGCTCAC AAAGACAACA 
GCAAAGACTG GAGGTGATGA CACTTCTCAG ACCATCAGTG CTGGAGAGAG CAGAGTGAAG 
AACAGTCCAG CCAAACCCAG TGACAACAGC ATGTCACAGC TGAGCCGCAG CAGCGTCGAC 
GCTGATCCAC TCAAGGAGCC TGAAAGTGCA GACAAACAGA AGAAGCAGAC AGGGAACCCA 
CATATTGAGC TGCAGTTCTC CGATGCAAAT GCTAAGTTCT ACTGCCGGAT CTACTATGCT 
GAAGAGTTCC ATAAAATGCG GGAGGAGATC ATGGAGAGCA CAGAGGATGA TTTTGTGCGA 
TCTCTTTCCC ACTGTGTCAA CTGGCAGGCG CGTGGTGGGA AATCTGGTGC AGTTTTCTAT 
GCCACTGAAG ATGATCGGTT TATTCTGAAG CAGATGCCCA GATTAGAGGT CCAGTCCTTT 
TTAGACTTTG CACCCCACTA CTTTACGTAC ATCACAGGAG CGGTTCACCT AAAGCGACCC 
ACAGCACTTG CTAAGATTCT GGGAGTGTAC CGCATCGGCT ACAAGAACTC CCAGAACAAC 
ACTGAGAAGA AGCTTGACCT GCTTGTGATG GAAAACCTTT TCTATGGACG AAAGATGGCA 
CAGGTGTTTG ACCTGAAAGG ATCGCTGAGA AACAGGAACG TGAAGACGGA CCAGGGAAAG 
GAGAGCTGTG AGGTGGTGCT CCTAGATGAG AACCTGCTCA AACTGGTGCA TGACAATCCC 
CTTTATATTC GCTCGCACTG CAAGGCCATC CTGCGTGCTG CCATCCACAG CGACGCGCTC 
TTCCTGTCCA GCCACCTCAT CATTGATTAC TCCCTGCTGG TGGGCCGAGA CGATTCCACA 
GATGAGCTAG TTGTGGGGAT CATAGATTAT ATCCGGACTT TCACTTGGGA TAAAAAGCTG 
GAGATGGTGG TCAAATCTAC TGGGATTCTT GGAGGTCAAG GTAAAATGCC CACAGTGGTG 
TCACCAGAGC TGTATCGAAC ACGTTTCTGT GAGGCCATGG ATAAATACTT CCTTATGGTC 
CCTGATCACT GGAGTGGACT TGGGCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167667.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167724.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.

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CloneID	ODa15217
Clone ID Related Accession (Same CDS sequence)	XM_005167667.4 , XM_005167667.3
Accession Version	XM_005167667.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGTC ATCAGGAATC TCAGAACAGC 
GGTCTCAATT CCAGACTGAC AGTGGTCCAA GAGGATATTG GAAAGTCACC TGCCAGGAAG 
AGGTCAGCTA GTGTGACCAA TTTGTCTTTG GACCGTTCCG GCTCATCCAT GGTTCCTGCC 
TATGAGAGTT CGGTTAGTCC ACAGAACAGC CGAGCTCTAC CCAAGACCGA CCACAGCGAA 
GAAGAGCGAA AGATCCTTCT AGATTCTTTT CAACTTAAAG ATATATGGAA GAAGATTTGC 
CACAACACTA CTGGGATGGA GTTCCAGGAC CATCGGTACT GGCTGCGCAC TTACCCCAAC 
TGCATTGTGG GTAAAGAGTT GGTCAACTGG CTTTTAAGAA ATGGCACTAT TTCCACTAGG 
GCTCAAGCCA TAGCTATAGG ACAGGCTTTG GTAGATGGCC GCTGGCTTGA CTGTGTAACC 
CATCATGATC AGATCTTCCG TGATGAGTAC GCCCTTTATC GCCCCCTTCA GAGCACAGAG 
TTCTCCGAAA CTCCTTCTCC AGACAGTGAC AGTGTTAATT CCCTGGAGGG ACACTCTGAA 
CCGTCATGGT TTAAAGACAT CAAGTTTGAT GACAGTGACA CCGAGCAGCT AGCAGATGAA 
AATGACTATG TCACACCAAG CTCGTCCAGC CCCAGCAAAA GAACATCTGT CAGCAGTTTC 
CACTCTGCAG TAGACAGTGA CTCGGCCGCC TCCATCAGTC TAAACATGGA GCAGGACAAC 
GTCAATTTCC ACATTAAGAA GCAGGCCAAG TACCCCCATG TGCCTCCCTA CCCAGCTGAG 
CAAAAAATGG AAGTCTTGCT CTCCGAAGAT GGAGGTCAGC ATATATCCAT CAGTGATGCA 
TTCATCAAAG AGTCTTTGTT CAACCGGAGG GTTGAGGAGA AAGCTAAAGA GATGCTCTTC 
ACTCCTCTCG GCTGGCATCA CAGCTCTCTG GATCAGCTGC GGGAAGAGAA TGGGGAAAAG 
AAAGCGATGG AGCGGTTGCT GTCAGCAAAT CACAGCCACA TGATGGCGCT GCTGCAGCAA 
CTGCTGTACA GTGAATCACT GTCTCTGTCG TGGCGTGACA TCATCGTACC TGTGGTGAGG 
CAGGTGGTGC AGACGGTGCG ACCGGATGTG CGCAGCTGTG ACGATGATAT GGACATCCGT 
CAGTTTGTCC ACATCAAGAA GATTCCAGGA GGAAAGAAGT TTGACTCTGC TGTAGTGAAT 
GGTTTTGTTT GCACAAAGAA TATCGCACAC AAAAAGATGA ACTCTTACAT CAAAAACCCC 
AAGATCCTTC TTCTCAAATG CTCCATTGAG TATCTATATA GAGAGGAGAC CAAGTTCACC 
TGCATTGACC CAATTGTTTT ACAGGAGCGT GAGTTTCTTA AGAACTATGT TCAGAGGATT 
GTTGATGTCC GACCAAACTT AGTCCTGGTG GAGAAGACTG TATCGCGAAT TGCTCAGGAC 
ATGCTACTAG AGCACGGCAT TGCCATTGTG ATTAATGTCA AACCTCAAGT ACTGGACCGT 
GTGAGTCGAA TGACTCAGGG AGATTTAGTC ATTTCAATGG ATCAGCTTCT TACAAAACCT 
CGTCTGGGTA CCTGCCACAA GTTTTACTTG CACTCCTTCC AGCTACCAAA TAGTGAAGTG 
AAAACCCTGA TGTTTTTTGA GGGCTGTCCT CCTCAGCTGG GCTGCACTAT TAAGCTCCGC 
GGAGCCTCAG AGTATGAGCT GGCACGGGTA AAAGAGATCA TCATTTTTAT GGTGTGTGTG 
GCATACCACT CGCAGCTAGA GATCTCTTTC CTCATGGATG AGTTCGCTAT GCCTCCCAGT 
CTGGCTGAGA GTTCTTCTTT CCCATGTCTG CTGGAAAGCA CCACTTTACG GGAAGAGGAT 
GAAACTAGTG GGGAACGGGA AAGCCAAGAC AATGATGAGT CTACACTGGG AGATGACAAT 
CTCACACTTG TTCCAGGAGA TGACTTTGAG ACAGGATTTA CAGGGACTAT CAAAGCCCAC 
AGTCGAGAGC CTTCCACCTC TGAATCTTCT CATAAAGATG GAGAAAGCCC CAGAATAAAT 
AAAAATGCTT CAATTACCTC CTTCTCTGGA GGGGAGGAAG AAAATATAAA GACCTCCACG 
CCACTTTCCT CCTTCTCTTC CACCTTTCCT CAGCCGGTGA CACCACCTTT CCTGATATCA 
GACGTAAAAG AAATGTCACA GGAAGTGGTC AAGGGGTCAG GTGAAGGGGA GAAGAACAAA 
TATCTGGAGT CGACTCTGGT CCATCAGGAC AGCACAAGCT CTGAGACCTC CCTTCCCCCA 
ACTCGGCTCT TTAGAGACCC CTTACAGGAC GACACAGGCC TGTTTGTGAC AGAGCATGTA 
GCTTCTTCAG ATGACCGCCT CAAATCCATC TCTGCTTTAT TCAAACAGGA GCTAAAAGAC 
ATCATCCTGT GCATTTCACC CTTTATTACC TTTCGAGAGC CTTTCTTGCT TACCCCAGCT 
GGACTGCGGT GTCCTAGCCG GGATTATTTC CCTGAACAGG TTTACCTTTC ACCTCTGCTG 
AACAAGGATT CAAAAGAACT GGATGGACGC CGCAAGAGGC AGCTGCTGAA AGAGTCCAGC 
CCAGGTTCTG GTAGCCTGAC CAATGGTTCC GTTTCACACC AGAGAGCCAT TCAGATCTTG 
CCCTGTCACA AACTCACTAG TGCCCGAATA GCAGAACAGG TAGGCAGCAG TCAGCAGCTG 
GCACGCATGT TAGCGGACTA CCGTGCCCAA GGAGGACGTA TCCGGCAAAG AGAGGGAACA 
CAATTTCGTG AAGCCCCTCC CCCAAAGGCG CCAATAAAGG CAGATAGTGA AGAAGATAAA 
GGTGCAGGAG TGATTGACAT GACCTGGTCT ACTAAGTTGG ACTGTTTAAA TCCAATCAAC 
CATCAGAGGC TCTGTGTTCT GTTCAGTAGC TCTTCAGCAC AGTCTAATAA TGCCCCAAAC 
CCCTGCGTCA GTCCCTGGAT TGTTACAATG GAGTTTTATG GAAAGAATGA CTTGACTCTT 
GGTGTGTTTC TGGAGAGATA CTGTTTTAGA CCATCTTACC AGTGCCCCAG CATGTACTGT 
GAGACCCCCA TGGTTCACCA CATCCGGCGC TTTGTGCACG GTAAAGGCTG CGTCCAGATT 
GTACTGAAAG AGCTGGACTC GCCTGTTCCT GGATATCAGC ACACAATCCT CAACTACTCA 
TGGTGTCGCA TCTGCAAACA GGTGACTCCT GTGGTCCCTT TGTCTAATGA CTCTTGGTCC 
ATGTCCTTCG CCAAGTACCT TGAGCTTCGT TTCTATGGTC ATCAGTACAC CCGTCGGGCT 
AACGCTGAGC CTTGTGGCCA CTCTATCCAT AAAGATTATC ACCAGTACTT CTCTTACAAC 
CAGATGGTGG CATCCTTCAG CTACATTCCA GTGAGGCTGT TAGAGATCTG TCTGCCTCCC 
CCGAAGATCA TCATCAGGAA CCAAGGTCCA TCGAAATCCA GCTTGCAGCA GGATCTCAAA 
GACTTCTCCC AGAAGGTGGC TCAGGTGTAC CTGGCTATAG ATGACCGTCT TACCTCCTTA 
AAAACGGACA CATTCAGCAA GACCAGAGAG GAAAAAATGG AGGACATGTT TGCACAGAAA 
GATATGGAGG AATCAGAGCT TCGGGGCTGG ATAGAGAAGT TACAAGTGCG TCTGCAGACG 
AGTGTGATTG ACTCTTCACA ACAACTACAG GCTGTTCTGG AGTCAGTGGT GGTCAAAAAA 
CAGGGCTTGT GTGAAACCCT GCAGTCCTGG AACAACAGAC TTCAAGAACT GTTCCAGCAA 
GAGAAAGGCA GAAAACGTCT ATCTGTTCCT CCTAGCCCAG GCAGACACAG ACAACCTACA 
ACAGATGAGA GCAAGACTAG TGCTTTGGAG TCTTCTCCTC GTAACCCTTC CCCAGTGGTC 
CCAAATGGAG ACAAAGAGGA CCGTCATCTC AATACATTTC CATCAAGTTC AGGGTCCTCG 
TCGTTACTAC AGTTACCATC TCCAGCTGAA CAACCTTCAG ATGTCATTAC AAGCGGACCA 
TCTTTCCCTG ATCAGGACTC TGTTAGCAAT CCAGAGGACA TGTTTGATGG ACACTTGCTT 
GGTTCCAATG ATAGTCAGGT AAAGGAAAAG TCCACCATGA AAACAATCCT GGCCAACCTG 
TTACCTGGAA ACAGCTACAA TCCCATCCCT TTTCCTTTTG ACCCAGACAA GCACTATCTG 
ATGTATGAGC ATGAGAGAGT TCCTATAGCC GTGTGTGAGA GAGAGCCCAG CTCCATAATT 
GCCTTTGCAC TCAGCTGCAA AGAGTATAAA ACTGCCCTTG AAGAGCTCAC AAAGACAACA 
GCAAAGACTG GAGGTGATGA CACTTCTCAG ACCATCAGTG CTGGAGAGAG CAGAGTGAAG 
AACAGTCCAG CCAAACCCAG TGACAACAGC ATGTCACAGC TGAGCCGCAG CAGCGTCGAC 
GCTGATCCAC TCAAGGAGCC TGAAAGTGCA GACAAACAGA AGAAGCAGAC AGGGAACCCA 
CATATTGAGC TGCAGTTCTC CGATGCAAAT GCTAAGTTCT ACTGCCGGAT CTACTATGCT 
GAAGAGTTCC ATAAAATGCG GGAGGAGATC ATGGAGAGCA CAGAGGATGA TTTTGTGCGA 
TCTCTTTCCC ACTGTGTCAA CTGGCAGGCG CGTGGTGGGA AATCTGGTGC AGTTTTCTAT 
GCCACTGAAG ATGATCGGTT TATTCTGAAG CAGATGCCCA GATTAGAGGT CCAGTCCTTT 
TTAGACTTTG CACCCCACTA CTTTACGTAC ATCACAGGAG CGGTTCACCT AAAGCGACCC 
ACAGCACTTG CTAAGATTCT GGGAGTGTAC CGCATCGGCT ACAAGAACTC CCAGAACAAC 
ACTGAGAAGA AGCTTGACCT GCTTGTGATG GAAAACCTTT TCTATGGACG AAAGATGGCA 
CAGGTGTTTG ACCTGAAAGG ATCGCTGAGA AACAGGAACG TGAAGACGGA CCAGGGAAAG 
GAGAGCTGTG AGGTGGTGCT CCTAGATGAG AACCTGCTCA AACTGGTGCA TGACAATCCC 
CTTTATATTC GCTCGCACTG CAAGGCCATC CTGCGTGCTG CCATCCACAG CGACGCGCTC 
TTCCTGTCCA GCCACCTCAT CATTGATTAC TCCCTGCTGG TGGGCCGAGA CGATTCCACA 
GATGAGCTAG TTGTGGGGAT CATAGATTAT ATCCGGACTT TCACTTGGGA TAAAAAGCTG 
GAGATGGTGG TCAAATCTAC TGGGATTCTT GGAGGTCAAG GTAAAATGCC CACAGTGGTG 
TCACCAGAGC TGTATCGAAC ACGTTTCTGT GAGGCCATGG ATAAATACTT CCTTATGGTC 
CCTGATCACT GGAGTGGACT TGGGCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167724.1
CDS	156..6431
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEKTGANIAPAKKLAKLEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQSHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167667.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167667.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAAAAAACAG GTGCAAACAT TGCACCAGCA AAAAAGCTTG CTAAGCTTGA GGAAGGACGG 
CCTCCTTCAG TGACGGAAAA AAGTCAATCG GCCTCATCAT CACCACAGGG GCCGCGACGC 
AACTGGTCTA GTCCCTCTCA TTCAATACAT GGCTCTGAAA CACACAGGAA ACATCCAGAA 
CTACTCAGAA GAACATCCGC TGCTTCAGAG GGTAGACGGA AATCAGAAGC TCCTCTGGGC 
AGCCACGATC CCAGAACAGC TGTCCAGCTT CGCACAGCTC TCAAGAGGCT CAAAGAGATC 
ATGGAAGGGA AAAGTCAGGA CAGTGATCTG AAACAGTACT GGATGCCAGA TAGCCAGTGT 
AAGGAGTGCT ATGACTGCAA TGAGAAATTC ACAACCTTCC GACGCCGTCA CCATTGTCGA 
CTATGTGGAC AAATATTCTG CAGCCGATGC TGCAACCAGG AAATTCCTGG CAAGTTCATG 
GGCTACACGG GCGATTTAAG GGCTTGTACA TACTGTCGTA AGATAGCATT AAGCTATGCT 
CACTCAACAG ACTCAAGCTC CATTGGAGAA GATCTAAGTG CTTTGTCGGA CTCGCCTTGC 
TCCGTCTGTG TTCTGGAGCC CACTGAACCA CGCACACCTG TAGGAGGTCG CAAAGCCAGC 
CGCAATATCT TCCTGGAAGA GGACCTAGCC TGGCAAAGTC ATCAGGAATC TCAGAACAGC 
GGTCTCAATT CCAGACTGAC AGTGGTCCAA GAGGATATTG GAAAGTCACC TGCCAGGAAG 
AGGTCAGCTA GTGTGACCAA TTTGTCTTTG GACCGTTCCG GCTCATCCAT GGTTCCTGCC 
TATGAGAGTT CGGTTAGTCC ACAGAACAGC CGAGCTCTAC CCAAGACCGA CCACAGCGAA 
GAAGAGCGAA AGATCCTTCT AGATTCTTTT CAACTTAAAG ATATATGGAA GAAGATTTGC 
CACAACACTA CTGGGATGGA GTTCCAGGAC CATCGGTACT GGCTGCGCAC TTACCCCAAC 
TGCATTGTGG GTAAAGAGTT GGTCAACTGG CTTTTAAGAA ATGGCACTAT TTCCACTAGG 
GCTCAAGCCA TAGCTATAGG ACAGGCTTTG GTAGATGGCC GCTGGCTTGA CTGTGTAACC 
CATCATGATC AGATCTTCCG TGATGAGTAC GCCCTTTATC GCCCCCTTCA GAGCACAGAG 
TTCTCCGAAA CTCCTTCTCC AGACAGTGAC AGTGTTAATT CCCTGGAGGG ACACTCTGAA 
CCGTCATGGT TTAAAGACAT CAAGTTTGAT GACAGTGACA CCGAGCAGCT AGCAGATGAA 
AATGACTATG TCACACCAAG CTCGTCCAGC CCCAGCAAAA GAACATCTGT CAGCAGTTTC 
CACTCTGCAG TAGACAGTGA CTCGGCCGCC TCCATCAGTC TAAACATGGA GCAGGACAAC 
GTCAATTTCC ACATTAAGAA GCAGGCCAAG TACCCCCATG TGCCTCCCTA CCCAGCTGAG 
CAAAAAATGG AAGTCTTGCT CTCCGAAGAT GGAGGTCAGC ATATATCCAT CAGTGATGCA 
TTCATCAAAG AGTCTTTGTT CAACCGGAGG GTTGAGGAGA AAGCTAAAGA GATGCTCTTC 
ACTCCTCTCG GCTGGCATCA CAGCTCTCTG GATCAGCTGC GGGAAGAGAA TGGGGAAAAG 
AAAGCGATGG AGCGGTTGCT GTCAGCAAAT CACAGCCACA TGATGGCGCT GCTGCAGCAA 
CTGCTGTACA GTGAATCACT GTCTCTGTCG TGGCGTGACA TCATCGTACC TGTGGTGAGG 
CAGGTGGTGC AGACGGTGCG ACCGGATGTG CGCAGCTGTG ACGATGATAT GGACATCCGT 
CAGTTTGTCC ACATCAAGAA GATTCCAGGA GGAAAGAAGT TTGACTCTGC TGTAGTGAAT 
GGTTTTGTTT GCACAAAGAA TATCGCACAC AAAAAGATGA ACTCTTACAT CAAAAACCCC 
AAGATCCTTC TTCTCAAATG CTCCATTGAG TATCTATATA GAGAGGAGAC CAAGTTCACC 
TGCATTGACC CAATTGTTTT ACAGGAGCGT GAGTTTCTTA AGAACTATGT TCAGAGGATT 
GTTGATGTCC GACCAAACTT AGTCCTGGTG GAGAAGACTG TATCGCGAAT TGCTCAGGAC 
ATGCTACTAG AGCACGGCAT TGCCATTGTG ATTAATGTCA AACCTCAAGT ACTGGACCGT 
GTGAGTCGAA TGACTCAGGG AGATTTAGTC ATTTCAATGG ATCAGCTTCT TACAAAACCT 
CGTCTGGGTA CCTGCCACAA GTTTTACTTG CACTCCTTCC AGCTACCAAA TAGTGAAGTG 
AAAACCCTGA TGTTTTTTGA GGGCTGTCCT CCTCAGCTGG GCTGCACTAT TAAGCTCCGC 
GGAGCCTCAG AGTATGAGCT GGCACGGGTA AAAGAGATCA TCATTTTTAT GGTGTGTGTG 
GCATACCACT CGCAGCTAGA GATCTCTTTC CTCATGGATG AGTTCGCTAT GCCTCCCAGT 
CTGGCTGAGA GTTCTTCTTT CCCATGTCTG CTGGAAAGCA CCACTTTACG GGAAGAGGAT 
GAAACTAGTG GGGAACGGGA AAGCCAAGAC AATGATGAGT CTACACTGGG AGATGACAAT 
CTCACACTTG TTCCAGGAGA TGACTTTGAG ACAGGATTTA CAGGGACTAT CAAAGCCCAC 
AGTCGAGAGC CTTCCACCTC TGAATCTTCT CATAAAGATG GAGAAAGCCC CAGAATAAAT 
AAAAATGCTT CAATTACCTC CTTCTCTGGA GGGGAGGAAG AAAATATAAA GACCTCCACG 
CCACTTTCCT CCTTCTCTTC CACCTTTCCT CAGCCGGTGA CACCACCTTT CCTGATATCA 
GACGTAAAAG AAATGTCACA GGAAGTGGTC AAGGGGTCAG GTGAAGGGGA GAAGAACAAA 
TATCTGGAGT CGACTCTGGT CCATCAGGAC AGCACAAGCT CTGAGACCTC CCTTCCCCCA 
ACTCGGCTCT TTAGAGACCC CTTACAGGAC GACACAGGCC TGTTTGTGAC AGAGCATGTA 
GCTTCTTCAG ATGACCGCCT CAAATCCATC TCTGCTTTAT TCAAACAGGA GCTAAAAGAC 
ATCATCCTGT GCATTTCACC CTTTATTACC TTTCGAGAGC CTTTCTTGCT TACCCCAGCT 
GGACTGCGGT GTCCTAGCCG GGATTATTTC CCTGAACAGG TTTACCTTTC ACCTCTGCTG 
AACAAGGATT CAAAAGAACT GGATGGACGC CGCAAGAGGC AGCTGCTGAA AGAGTCCAGC 
CCAGGTTCTG GTAGCCTGAC CAATGGTTCC GTTTCACACC AGAGAGCCAT TCAGATCTTG 
CCCTGTCACA AACTCACTAG TGCCCGAATA GCAGAACAGG TAGGCAGCAG TCAGCAGCTG 
GCACGCATGT TAGCGGACTA CCGTGCCCAA GGAGGACGTA TCCGGCAAAG AGAGGGAACA 
CAATTTCGTG AAGCCCCTCC CCCAAAGGCG CCAATAAAGG CAGATAGTGA AGAAGATAAA 
GGTGCAGGAG TGATTGACAT GACCTGGTCT ACTAAGTTGG ACTGTTTAAA TCCAATCAAC 
CATCAGAGGC TCTGTGTTCT GTTCAGTAGC TCTTCAGCAC AGTCTAATAA TGCCCCAAAC 
CCCTGCGTCA GTCCCTGGAT TGTTACAATG GAGTTTTATG GAAAGAATGA CTTGACTCTT 
GGTGTGTTTC TGGAGAGATA CTGTTTTAGA CCATCTTACC AGTGCCCCAG CATGTACTGT 
GAGACCCCCA TGGTTCACCA CATCCGGCGC TTTGTGCACG GTAAAGGCTG CGTCCAGATT 
GTACTGAAAG AGCTGGACTC GCCTGTTCCT GGATATCAGC ACACAATCCT CAACTACTCA 
TGGTGTCGCA TCTGCAAACA GGTGACTCCT GTGGTCCCTT TGTCTAATGA CTCTTGGTCC 
ATGTCCTTCG CCAAGTACCT TGAGCTTCGT TTCTATGGTC ATCAGTACAC CCGTCGGGCT 
AACGCTGAGC CTTGTGGCCA CTCTATCCAT AAAGATTATC ACCAGTACTT CTCTTACAAC 
CAGATGGTGG CATCCTTCAG CTACATTCCA GTGAGGCTGT TAGAGATCTG TCTGCCTCCC 
CCGAAGATCA TCATCAGGAA CCAAGGTCCA TCGAAATCCA GCTTGCAGCA GGATCTCAAA 
GACTTCTCCC AGAAGGTGGC TCAGGTGTAC CTGGCTATAG ATGACCGTCT TACCTCCTTA 
AAAACGGACA CATTCAGCAA GACCAGAGAG GAAAAAATGG AGGACATGTT TGCACAGAAA 
GATATGGAGG AATCAGAGCT TCGGGGCTGG ATAGAGAAGT TACAAGTGCG TCTGCAGACG 
AGTGTGATTG ACTCTTCACA ACAACTACAG GCTGTTCTGG AGTCAGTGGT GGTCAAAAAA 
CAGGGCTTGT GTGAAACCCT GCAGTCCTGG AACAACAGAC TTCAAGAACT GTTCCAGCAA 
GAGAAAGGCA GAAAACGTCT ATCTGTTCCT CCTAGCCCAG GCAGACACAG ACAACCTACA 
ACAGATGAGA GCAAGACTAG TGCTTTGGAG TCTTCTCCTC GTAACCCTTC CCCAGTGGTC 
CCAAATGGAG ACAAAGAGGA CCGTCATCTC AATACATTTC CATCAAGTTC AGGGTCCTCG 
TCGTTACTAC AGTTACCATC TCCAGCTGAA CAACCTTCAG ATGTCATTAC AAGCGGACCA 
TCTTTCCCTG ATCAGGACTC TGTTAGCAAT CCAGAGGACA TGTTTGATGG ACACTTGCTT 
GGTTCCAATG ATAGTCAGGT AAAGGAAAAG TCCACCATGA AAACAATCCT GGCCAACCTG 
TTACCTGGAA ACAGCTACAA TCCCATCCCT TTTCCTTTTG ACCCAGACAA GCACTATCTG 
ATGTATGAGC ATGAGAGAGT TCCTATAGCC GTGTGTGAGA GAGAGCCCAG CTCCATAATT 
GCCTTTGCAC TCAGCTGCAA AGAGTATAAA ACTGCCCTTG AAGAGCTCAC AAAGACAACA 
GCAAAGACTG GAGGTGATGA CACTTCTCAG ACCATCAGTG CTGGAGAGAG CAGAGTGAAG 
AACAGTCCAG CCAAACCCAG TGACAACAGC ATGTCACAGC TGAGCCGCAG CAGCGTCGAC 
GCTGATCCAC TCAAGGAGCC TGAAAGTGCA GACAAACAGA AGAAGCAGAC AGGGAACCCA 
CATATTGAGC TGCAGTTCTC CGATGCAAAT GCTAAGTTCT ACTGCCGGAT CTACTATGCT 
GAAGAGTTCC ATAAAATGCG GGAGGAGATC ATGGAGAGCA CAGAGGATGA TTTTGTGCGA 
TCTCTTTCCC ACTGTGTCAA CTGGCAGGCG CGTGGTGGGA AATCTGGTGC AGTTTTCTAT 
GCCACTGAAG ATGATCGGTT TATTCTGAAG CAGATGCCCA GATTAGAGGT CCAGTCCTTT 
TTAGACTTTG CACCCCACTA CTTTACGTAC ATCACAGGAG CGGTTCACCT AAAGCGACCC 
ACAGCACTTG CTAAGATTCT GGGAGTGTAC CGCATCGGCT ACAAGAACTC CCAGAACAAC 
ACTGAGAAGA AGCTTGACCT GCTTGTGATG GAAAACCTTT TCTATGGACG AAAGATGGCA 
CAGGTGTTTG ACCTGAAAGG ATCGCTGAGA AACAGGAACG TGAAGACGGA CCAGGGAAAG 
GAGAGCTGTG AGGTGGTGCT CCTAGATGAG AACCTGCTCA AACTGGTGCA TGACAATCCC 
CTTTATATTC GCTCGCACTG CAAGGCCATC CTGCGTGCTG CCATCCACAG CGACGCGCTC 
TTCCTGTCCA GCCACCTCAT CATTGATTAC TCCCTGCTGG TGGGCCGAGA CGATTCCACA 
GATGAGCTAG TTGTGGGGAT CATAGATTAT ATCCGGACTT TCACTTGGGA TAAAAAGCTG 
GAGATGGTGG TCAAATCTAC TGGGATTCTT GGAGGTCAAG GTAAAATGCC CACAGTGGTG 
TCACCAGAGC TGTATCGAAC ACGTTTCTGT GAGGCCATGG ATAAATACTT CCTTATGGTC 
CCTGATCACT GGAGTGGACT TGGGCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167669.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167726.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.

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CloneID	ODa15219
Clone ID Related Accession (Same CDS sequence)	XM_005167669.3 , XM_005167669.4
Accession Version	XM_005167669.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAATGGA AGTCTTGCTC 
TCCGAAGATG GAGGTCAGCA TATATCCATC AGTGATGCAT TCATCAAAGA GTCTTTGTTC 
AACCGGAGGG TTGAGGAGAA AGCTAAAGAG ATGCTCTTCA CTCCTCTCGG CTGGCATCAC 
AGCTCTCTGG ATCAGCTGCG GGAAGAGAAT GGGGAAAAGA AAGCGATGGA GCGGTTGCTG 
TCAGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAAC TGCTGTACAG TGAATCACTG 
TCTCTGTCGT GGCGTGACAT CATCGTACCT GTGGTGAGGC AGGTGGTGCA GACGGTGCGA 
CCGGATGTGC GCAGCTGTGA CGATGATATG GACATCCGTC AGTTTGTCCA CATCAAGAAG 
ATTCCAGGAG GAAAGAAGTT TGACTCTGCT GTAGTGAATG GTTTTGTTTG CACAAAGAAT 
ATCGCACACA AAAAGATGAA CTCTTACATC AAAAACCCCA AGATCCTTCT TCTCAAATGC 
TCCATTGAGT ATCTATATAG AGAGGAGACC AAGTTCACCT GCATTGACCC AATTGTTTTA 
CAGGAGCGTG AGTTTCTTAA GAACTATGTT CAGAGGATTG TTGATGTCCG ACCAAACTTA 
GTCCTGGTGG AGAAGACTGT ATCGCGAATT GCTCAGGACA TGCTACTAGA GCACGGCATT 
GCCATTGTGA TTAATGTCAA ACCTCAAGTA CTGGACCGTG TGAGTCGAAT GACTCAGGGA 
GATTTAGTCA TTTCAATGGA TCAGCTTCTT ACAAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACTTGC ACTCCTTCCA GCTACCAAAT AGTGAAGTGA AAACCCTGAT GTTTTTTGAG 
GGCTGTCCTC CTCAGCTGGG CTGCACTATT AAGCTCCGCG GAGCCTCAGA GTATGAGCTG 
GCACGGGTAA AAGAGATCAT CATTTTTATG GTGTGTGTGG CATACCACTC GCAGCTAGAG 
ATCTCTTTCC TCATGGATGA GTTCGCTATG CCTCCCAGTC TGGCTGAGAG TTCTTCTTTC 
CCATGTCTGC TGGAAAGCAC CACTTTACGG GAAGAGGATG AAACTAGTGG GGAACGGGAA 
AGCCAAGACA ATGATGAGTC TACACTGGGA GATGACAATC TCACACTTGT TCCAGGAGAT 
GACTTTGAGA CAGGATTTAC AGGGACTATC AAAGCCCACA GTCGAGAGCC TTCCACCTCT 
GAATCTTCTC ATAAAGATGG AGAAAGCCCC AGAATAAATA AAAATGCTTC AATTACCTCC 
TTCTCTGGAG GGGAGGAAGA AAATATAAAG ACCTCCACGC CACTTTCCTC CTTCTCTTCC 
ACCTTTCCTC AGCCGGTGAC ACCACCTTTC CTGATATCAG ACGTAAAAGA AATGTCACAG 
GAAGTGGTCA AGGGGTCAGG TGAAGGGGAG AAGAACAAAT ATCTGGAGTC GACTCTGGTC 
CATCAGGACA GCACAAGCTC TGAGACCTCC CTTCCCCCAA CTCGGCTCTT TAGAGACCCC 
TTACAGGACG ACACAGGCCT GTTTGTGACA GAGCATGTAG CTTCTTCAGA TGACCGCCTC 
AAATCCATCT CTGCTTTATT CAAACAGGAG CTAAAAGACA TCATCCTGTG CATTTCACCC 
TTTATTACCT TTCGAGAGCC TTTCTTGCTT ACCCCAGCTG GACTGCGGTG TCCTAGCCGG 
GATTATTTCC CTGAACAGGT TTACCTTTCA CCTCTGCTGA ACAAGGATTC AAAAGAACTG 
GATGGACGCC GCAAGAGGCA GCTGCTGAAA GAGTCCAGCC CAGGTTCTGG TAGCCTGACC 
AATGGTTCCG TTTCACACCA GAGAGCCATT CAGATCTTGC CCTGTCACAA ACTCACTAGT 
GCCCGAATAG CAGAACAGGT AGGCAGCAGT CAGCAGCTGG CACGCATGTT AGCGGACTAC 
CGTGCCCAAG GAGGACGTAT CCGGCAAAGA GAGGGAACAC AATTTCGTGA AGCCCCTCCC 
CCAAAGGCGC CAATAAAGGC AGATAGTGAA GAAGATAAAG GTGCAGGAGT GATTGACATG 
ACCTGGTCTA CTAAGTTGGA CTGTTTAAAT CCAATCAACC ATCAGAGGCT CTGTGTTCTG 
TTCAGTAGCT CTTCAGCACA GTCTAATAAT GCCCCAAACC CCTGCGTCAG TCCCTGGATT 
GTTACAATGG AGTTTTATGG AAAGAATGAC TTGACTCTTG GTGTGTTTCT GGAGAGATAC 
TGTTTTAGAC CATCTTACCA GTGCCCCAGC ATGTACTGTG AGACCCCCAT GGTTCACCAC 
ATCCGGCGCT TTGTGCACGG TAAAGGCTGC GTCCAGATTG TACTGAAAGA GCTGGACTCG 
CCTGTTCCTG GATATCAGCA CACAATCCTC AACTACTCAT GGTGTCGCAT CTGCAAACAG 
GTGACTCCTG TGGTCCCTTT GTCTAATGAC TCTTGGTCCA TGTCCTTCGC CAAGTACCTT 
GAGCTTCGTT TCTATGGTCA TCAGTACACC CGTCGGGCTA ACGCTGAGCC TTGTGGCCAC 
TCTATCCATA AAGATTATCA CCAGTACTTC TCTTACAACC AGATGGTGGC ATCCTTCAGC 
TACATTCCAG TGAGGCTGTT AGAGATCTGT CTGCCTCCCC CGAAGATCAT CATCAGGAAC 
CAAGGTCCAT CGAAATCCAG CTTGCAGCAG GATCTCAAAG ACTTCTCCCA GAAGGTGGCT 
CAGGTGTACC TGGCTATAGA TGACCGTCTT ACCTCCTTAA AAACGGACAC ATTCAGCAAG 
ACCAGAGAGG AAAAAATGGA GGACATGTTT GCACAGAAAG ATATGGAGGA ATCAGAGCTT 
CGGGGCTGGA TAGAGAAGTT ACAAGTGCGT CTGCAGACGA GTGTGATTGA CTCTTCACAA 
CAACTACAGG CTGTTCTGGA GTCAGTGGTG GTCAAAAAAC AGGGCTTGTG TGAAACCCTG 
CAGTCCTGGA ACAACAGACT TCAAGAACTG TTCCAGCAAG AGAAAGGCAG AAAACGTCTA 
TCTGTTCCTC CTAGCCCAGG CAGACACAGA CAACCTACAA CAGATGAGAG CAAGACTAGT 
GCTTTGGAGT CTTCTCCTCG TAACCCTTCC CCAGTGGTCC CAAATGGAGA CAAAGAGGAC 
CGTCATCTCA ATACATTTCC ATCAAGTTCA GGGTCCTCGT CGTTACTACA GTTACCATCT 
CCAGCTGAAC AACCTTCAGA TGTCATTACA AGCGGACCAT CTTTCCCTGA TCAGGACTCT 
GTTAGCAATC CAGAGGACAT GTTTGATGGA CACTTGCTTG GTTCCAATGA TAGTCAGGTA 
AAGGAAAAGT CCACCATGAA AACAATCCTG GCCAACCTGT TACCTGGAAA CAGCTACAAT 
CCCATCCCTT TTCCTTTTGA CCCAGACAAG CACTATCTGA TGTATGAGCA TGAGAGAGTT 
CCTATAGCCG TGTGTGAGAG AGAGCCCAGC TCCATAATTG CCTTTGCACT CAGCTGCAAA 
GAGTATAAAA CTGCCCTTGA AGAGCTCACA AAGACAACAG CAAAGACTGG AGGTGATGAC 
ACTTCTCAGA CCATCAGTGC TGGAGAGAGC AGAGTGAAGA ACAGTCCAGC CAAACCCAGT 
GACAACAGCA TGTCACAGCT GAGCCGCAGC AGCGTCGACG CTGATCCACT CAAGGAGCCT 
GAAAGTGCAG ACAAACAGAA GAAGCAGACA GGGAACCCAC ATATTGAGCT GCAGTTCTCC 
GATGCAAATG CTAAGTTCTA CTGCCGGATC TACTATGCTG AAGAGTTCCA TAAAATGCGG 
GAGGAGATCA TGGAGAGCAC AGAGGATGAT TTTGTGCGAT CTCTTTCCCA CTGTGTCAAC 
TGGCAGGCGC GTGGTGGGAA ATCTGGTGCA GTTTTCTATG CCACTGAAGA TGATCGGTTT 
ATTCTGAAGC AGATGCCCAG ATTAGAGGTC CAGTCCTTTT TAGACTTTGC ACCCCACTAC 
TTTACGTACA TCACAGGAGC GGTTCACCTA AAGCGACCCA CAGCACTTGC TAAGATTCTG 
GGAGTGTACC GCATCGGCTA CAAGAACTCC CAGAACAACA CTGAGAAGAA GCTTGACCTG 
CTTGTGATGG AAAACCTTTT CTATGGACGA AAGATGGCAC AGGTGTTTGA CCTGAAAGGA 
TCGCTGAGAA ACAGGAACGT GAAGACGGAC CAGGGAAAGG AGAGCTGTGA GGTGGTGCTC 
CTAGATGAGA ACCTGCTCAA ACTGGTGCAT GACAATCCCC TTTATATTCG CTCGCACTGC 
AAGGCCATCC TGCGTGCTGC CATCCACAGC GACGCGCTCT TCCTGTCCAG CCACCTCATC 
ATTGATTACT CCCTGCTGGT GGGCCGAGAC GATTCCACAG ATGAGCTAGT TGTGGGGATC 
ATAGATTATA TCCGGACTTT CACTTGGGAT AAAAAGCTGG AGATGGTGGT CAAATCTACT 
GGGATTCTTG GAGGTCAAGG TAAAATGCCC ACAGTGGTGT CACCAGAGCT GTATCGAACA 
CGTTTCTGTG AGGCCATGGA TAAATACTTC CTTATGGTCC CTGATCACTG GAGTGGACTT 
GGGCTCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167726.1
CDS	156..6410
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167669.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167669.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAATGGA AGTCTTGCTC 
TCCGAAGATG GAGGTCAGCA TATATCCATC AGTGATGCAT TCATCAAAGA GTCTTTGTTC 
AACCGGAGGG TTGAGGAGAA AGCTAAAGAG ATGCTCTTCA CTCCTCTCGG CTGGCATCAC 
AGCTCTCTGG ATCAGCTGCG GGAAGAGAAT GGGGAAAAGA AAGCGATGGA GCGGTTGCTG 
TCAGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAAC TGCTGTACAG TGAATCACTG 
TCTCTGTCGT GGCGTGACAT CATCGTACCT GTGGTGAGGC AGGTGGTGCA GACGGTGCGA 
CCGGATGTGC GCAGCTGTGA CGATGATATG GACATCCGTC AGTTTGTCCA CATCAAGAAG 
ATTCCAGGAG GAAAGAAGTT TGACTCTGCT GTAGTGAATG GTTTTGTTTG CACAAAGAAT 
ATCGCACACA AAAAGATGAA CTCTTACATC AAAAACCCCA AGATCCTTCT TCTCAAATGC 
TCCATTGAGT ATCTATATAG AGAGGAGACC AAGTTCACCT GCATTGACCC AATTGTTTTA 
CAGGAGCGTG AGTTTCTTAA GAACTATGTT CAGAGGATTG TTGATGTCCG ACCAAACTTA 
GTCCTGGTGG AGAAGACTGT ATCGCGAATT GCTCAGGACA TGCTACTAGA GCACGGCATT 
GCCATTGTGA TTAATGTCAA ACCTCAAGTA CTGGACCGTG TGAGTCGAAT GACTCAGGGA 
GATTTAGTCA TTTCAATGGA TCAGCTTCTT ACAAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACTTGC ACTCCTTCCA GCTACCAAAT AGTGAAGTGA AAACCCTGAT GTTTTTTGAG 
GGCTGTCCTC CTCAGCTGGG CTGCACTATT AAGCTCCGCG GAGCCTCAGA GTATGAGCTG 
GCACGGGTAA AAGAGATCAT CATTTTTATG GTGTGTGTGG CATACCACTC GCAGCTAGAG 
ATCTCTTTCC TCATGGATGA GTTCGCTATG CCTCCCAGTC TGGCTGAGAG TTCTTCTTTC 
CCATGTCTGC TGGAAAGCAC CACTTTACGG GAAGAGGATG AAACTAGTGG GGAACGGGAA 
AGCCAAGACA ATGATGAGTC TACACTGGGA GATGACAATC TCACACTTGT TCCAGGAGAT 
GACTTTGAGA CAGGATTTAC AGGGACTATC AAAGCCCACA GTCGAGAGCC TTCCACCTCT 
GAATCTTCTC ATAAAGATGG AGAAAGCCCC AGAATAAATA AAAATGCTTC AATTACCTCC 
TTCTCTGGAG GGGAGGAAGA AAATATAAAG ACCTCCACGC CACTTTCCTC CTTCTCTTCC 
ACCTTTCCTC AGCCGGTGAC ACCACCTTTC CTGATATCAG ACGTAAAAGA AATGTCACAG 
GAAGTGGTCA AGGGGTCAGG TGAAGGGGAG AAGAACAAAT ATCTGGAGTC GACTCTGGTC 
CATCAGGACA GCACAAGCTC TGAGACCTCC CTTCCCCCAA CTCGGCTCTT TAGAGACCCC 
TTACAGGACG ACACAGGCCT GTTTGTGACA GAGCATGTAG CTTCTTCAGA TGACCGCCTC 
AAATCCATCT CTGCTTTATT CAAACAGGAG CTAAAAGACA TCATCCTGTG CATTTCACCC 
TTTATTACCT TTCGAGAGCC TTTCTTGCTT ACCCCAGCTG GACTGCGGTG TCCTAGCCGG 
GATTATTTCC CTGAACAGGT TTACCTTTCA CCTCTGCTGA ACAAGGATTC AAAAGAACTG 
GATGGACGCC GCAAGAGGCA GCTGCTGAAA GAGTCCAGCC CAGGTTCTGG TAGCCTGACC 
AATGGTTCCG TTTCACACCA GAGAGCCATT CAGATCTTGC CCTGTCACAA ACTCACTAGT 
GCCCGAATAG CAGAACAGGT AGGCAGCAGT CAGCAGCTGG CACGCATGTT AGCGGACTAC 
CGTGCCCAAG GAGGACGTAT CCGGCAAAGA GAGGGAACAC AATTTCGTGA AGCCCCTCCC 
CCAAAGGCGC CAATAAAGGC AGATAGTGAA GAAGATAAAG GTGCAGGAGT GATTGACATG 
ACCTGGTCTA CTAAGTTGGA CTGTTTAAAT CCAATCAACC ATCAGAGGCT CTGTGTTCTG 
TTCAGTAGCT CTTCAGCACA GTCTAATAAT GCCCCAAACC CCTGCGTCAG TCCCTGGATT 
GTTACAATGG AGTTTTATGG AAAGAATGAC TTGACTCTTG GTGTGTTTCT GGAGAGATAC 
TGTTTTAGAC CATCTTACCA GTGCCCCAGC ATGTACTGTG AGACCCCCAT GGTTCACCAC 
ATCCGGCGCT TTGTGCACGG TAAAGGCTGC GTCCAGATTG TACTGAAAGA GCTGGACTCG 
CCTGTTCCTG GATATCAGCA CACAATCCTC AACTACTCAT GGTGTCGCAT CTGCAAACAG 
GTGACTCCTG TGGTCCCTTT GTCTAATGAC TCTTGGTCCA TGTCCTTCGC CAAGTACCTT 
GAGCTTCGTT TCTATGGTCA TCAGTACACC CGTCGGGCTA ACGCTGAGCC TTGTGGCCAC 
TCTATCCATA AAGATTATCA CCAGTACTTC TCTTACAACC AGATGGTGGC ATCCTTCAGC 
TACATTCCAG TGAGGCTGTT AGAGATCTGT CTGCCTCCCC CGAAGATCAT CATCAGGAAC 
CAAGGTCCAT CGAAATCCAG CTTGCAGCAG GATCTCAAAG ACTTCTCCCA GAAGGTGGCT 
CAGGTGTACC TGGCTATAGA TGACCGTCTT ACCTCCTTAA AAACGGACAC ATTCAGCAAG 
ACCAGAGAGG AAAAAATGGA GGACATGTTT GCACAGAAAG ATATGGAGGA ATCAGAGCTT 
CGGGGCTGGA TAGAGAAGTT ACAAGTGCGT CTGCAGACGA GTGTGATTGA CTCTTCACAA 
CAACTACAGG CTGTTCTGGA GTCAGTGGTG GTCAAAAAAC AGGGCTTGTG TGAAACCCTG 
CAGTCCTGGA ACAACAGACT TCAAGAACTG TTCCAGCAAG AGAAAGGCAG AAAACGTCTA 
TCTGTTCCTC CTAGCCCAGG CAGACACAGA CAACCTACAA CAGATGAGAG CAAGACTAGT 
GCTTTGGAGT CTTCTCCTCG TAACCCTTCC CCAGTGGTCC CAAATGGAGA CAAAGAGGAC 
CGTCATCTCA ATACATTTCC ATCAAGTTCA GGGTCCTCGT CGTTACTACA GTTACCATCT 
CCAGCTGAAC AACCTTCAGA TGTCATTACA AGCGGACCAT CTTTCCCTGA TCAGGACTCT 
GTTAGCAATC CAGAGGACAT GTTTGATGGA CACTTGCTTG GTTCCAATGA TAGTCAGGTA 
AAGGAAAAGT CCACCATGAA AACAATCCTG GCCAACCTGT TACCTGGAAA CAGCTACAAT 
CCCATCCCTT TTCCTTTTGA CCCAGACAAG CACTATCTGA TGTATGAGCA TGAGAGAGTT 
CCTATAGCCG TGTGTGAGAG AGAGCCCAGC TCCATAATTG CCTTTGCACT CAGCTGCAAA 
GAGTATAAAA CTGCCCTTGA AGAGCTCACA AAGACAACAG CAAAGACTGG AGGTGATGAC 
ACTTCTCAGA CCATCAGTGC TGGAGAGAGC AGAGTGAAGA ACAGTCCAGC CAAACCCAGT 
GACAACAGCA TGTCACAGCT GAGCCGCAGC AGCGTCGACG CTGATCCACT CAAGGAGCCT 
GAAAGTGCAG ACAAACAGAA GAAGCAGACA GGGAACCCAC ATATTGAGCT GCAGTTCTCC 
GATGCAAATG CTAAGTTCTA CTGCCGGATC TACTATGCTG AAGAGTTCCA TAAAATGCGG 
GAGGAGATCA TGGAGAGCAC AGAGGATGAT TTTGTGCGAT CTCTTTCCCA CTGTGTCAAC 
TGGCAGGCGC GTGGTGGGAA ATCTGGTGCA GTTTTCTATG CCACTGAAGA TGATCGGTTT 
ATTCTGAAGC AGATGCCCAG ATTAGAGGTC CAGTCCTTTT TAGACTTTGC ACCCCACTAC 
TTTACGTACA TCACAGGAGC GGTTCACCTA AAGCGACCCA CAGCACTTGC TAAGATTCTG 
GGAGTGTACC GCATCGGCTA CAAGAACTCC CAGAACAACA CTGAGAAGAA GCTTGACCTG 
CTTGTGATGG AAAACCTTTT CTATGGACGA AAGATGGCAC AGGTGTTTGA CCTGAAAGGA 
TCGCTGAGAA ACAGGAACGT GAAGACGGAC CAGGGAAAGG AGAGCTGTGA GGTGGTGCTC 
CTAGATGAGA ACCTGCTCAA ACTGGTGCAT GACAATCCCC TTTATATTCG CTCGCACTGC 
AAGGCCATCC TGCGTGCTGC CATCCACAGC GACGCGCTCT TCCTGTCCAG CCACCTCATC 
ATTGATTACT CCCTGCTGGT GGGCCGAGAC GATTCCACAG ATGAGCTAGT TGTGGGGATC 
ATAGATTATA TCCGGACTTT CACTTGGGAT AAAAAGCTGG AGATGGTGGT CAAATCTACT 
GGGATTCTTG GAGGTCAAGG TAAAATGCCC ACAGTGGTGT CACCAGAGCT GTATCGAACA 
CGTTTCTGTG AGGCCATGGA TAAATACTTC CTTATGGTCC CTGATCACTG GAGTGGACTT 
GGGCTCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167669.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167726.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.

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CloneID	ODa15219
Clone ID Related Accession (Same CDS sequence)	XM_005167669.3 , XM_005167669.4
Accession Version	XM_005167669.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167669.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAATGGA AGTCTTGCTC 
TCCGAAGATG GAGGTCAGCA TATATCCATC AGTGATGCAT TCATCAAAGA GTCTTTGTTC 
AACCGGAGGG TTGAGGAGAA AGCTAAAGAG ATGCTCTTCA CTCCTCTCGG CTGGCATCAC 
AGCTCTCTGG ATCAGCTGCG GGAAGAGAAT GGGGAAAAGA AAGCGATGGA GCGGTTGCTG 
TCAGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAAC TGCTGTACAG TGAATCACTG 
TCTCTGTCGT GGCGTGACAT CATCGTACCT GTGGTGAGGC AGGTGGTGCA GACGGTGCGA 
CCGGATGTGC GCAGCTGTGA CGATGATATG GACATCCGTC AGTTTGTCCA CATCAAGAAG 
ATTCCAGGAG GAAAGAAGTT TGACTCTGCT GTAGTGAATG GTTTTGTTTG CACAAAGAAT 
ATCGCACACA AAAAGATGAA CTCTTACATC AAAAACCCCA AGATCCTTCT TCTCAAATGC 
TCCATTGAGT ATCTATATAG AGAGGAGACC AAGTTCACCT GCATTGACCC AATTGTTTTA 
CAGGAGCGTG AGTTTCTTAA GAACTATGTT CAGAGGATTG TTGATGTCCG ACCAAACTTA 
GTCCTGGTGG AGAAGACTGT ATCGCGAATT GCTCAGGACA TGCTACTAGA GCACGGCATT 
GCCATTGTGA TTAATGTCAA ACCTCAAGTA CTGGACCGTG TGAGTCGAAT GACTCAGGGA 
GATTTAGTCA TTTCAATGGA TCAGCTTCTT ACAAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACTTGC ACTCCTTCCA GCTACCAAAT AGTGAAGTGA AAACCCTGAT GTTTTTTGAG 
GGCTGTCCTC CTCAGCTGGG CTGCACTATT AAGCTCCGCG GAGCCTCAGA GTATGAGCTG 
GCACGGGTAA AAGAGATCAT CATTTTTATG GTGTGTGTGG CATACCACTC GCAGCTAGAG 
ATCTCTTTCC TCATGGATGA GTTCGCTATG CCTCCCAGTC TGGCTGAGAG TTCTTCTTTC 
CCATGTCTGC TGGAAAGCAC CACTTTACGG GAAGAGGATG AAACTAGTGG GGAACGGGAA 
AGCCAAGACA ATGATGAGTC TACACTGGGA GATGACAATC TCACACTTGT TCCAGGAGAT 
GACTTTGAGA CAGGATTTAC AGGGACTATC AAAGCCCACA GTCGAGAGCC TTCCACCTCT 
GAATCTTCTC ATAAAGATGG AGAAAGCCCC AGAATAAATA AAAATGCTTC AATTACCTCC 
TTCTCTGGAG GGGAGGAAGA AAATATAAAG ACCTCCACGC CACTTTCCTC CTTCTCTTCC 
ACCTTTCCTC AGCCGGTGAC ACCACCTTTC CTGATATCAG ACGTAAAAGA AATGTCACAG 
GAAGTGGTCA AGGGGTCAGG TGAAGGGGAG AAGAACAAAT ATCTGGAGTC GACTCTGGTC 
CATCAGGACA GCACAAGCTC TGAGACCTCC CTTCCCCCAA CTCGGCTCTT TAGAGACCCC 
TTACAGGACG ACACAGGCCT GTTTGTGACA GAGCATGTAG CTTCTTCAGA TGACCGCCTC 
AAATCCATCT CTGCTTTATT CAAACAGGAG CTAAAAGACA TCATCCTGTG CATTTCACCC 
TTTATTACCT TTCGAGAGCC TTTCTTGCTT ACCCCAGCTG GACTGCGGTG TCCTAGCCGG 
GATTATTTCC CTGAACAGGT TTACCTTTCA CCTCTGCTGA ACAAGGATTC AAAAGAACTG 
GATGGACGCC GCAAGAGGCA GCTGCTGAAA GAGTCCAGCC CAGGTTCTGG TAGCCTGACC 
AATGGTTCCG TTTCACACCA GAGAGCCATT CAGATCTTGC CCTGTCACAA ACTCACTAGT 
GCCCGAATAG CAGAACAGGT AGGCAGCAGT CAGCAGCTGG CACGCATGTT AGCGGACTAC 
CGTGCCCAAG GAGGACGTAT CCGGCAAAGA GAGGGAACAC AATTTCGTGA AGCCCCTCCC 
CCAAAGGCGC CAATAAAGGC AGATAGTGAA GAAGATAAAG GTGCAGGAGT GATTGACATG 
ACCTGGTCTA CTAAGTTGGA CTGTTTAAAT CCAATCAACC ATCAGAGGCT CTGTGTTCTG 
TTCAGTAGCT CTTCAGCACA GTCTAATAAT GCCCCAAACC CCTGCGTCAG TCCCTGGATT 
GTTACAATGG AGTTTTATGG AAAGAATGAC TTGACTCTTG GTGTGTTTCT GGAGAGATAC 
TGTTTTAGAC CATCTTACCA GTGCCCCAGC ATGTACTGTG AGACCCCCAT GGTTCACCAC 
ATCCGGCGCT TTGTGCACGG TAAAGGCTGC GTCCAGATTG TACTGAAAGA GCTGGACTCG 
CCTGTTCCTG GATATCAGCA CACAATCCTC AACTACTCAT GGTGTCGCAT CTGCAAACAG 
GTGACTCCTG TGGTCCCTTT GTCTAATGAC TCTTGGTCCA TGTCCTTCGC CAAGTACCTT 
GAGCTTCGTT TCTATGGTCA TCAGTACACC CGTCGGGCTA ACGCTGAGCC TTGTGGCCAC 
TCTATCCATA AAGATTATCA CCAGTACTTC TCTTACAACC AGATGGTGGC ATCCTTCAGC 
TACATTCCAG TGAGGCTGTT AGAGATCTGT CTGCCTCCCC CGAAGATCAT CATCAGGAAC 
CAAGGTCCAT CGAAATCCAG CTTGCAGCAG GATCTCAAAG ACTTCTCCCA GAAGGTGGCT 
CAGGTGTACC TGGCTATAGA TGACCGTCTT ACCTCCTTAA AAACGGACAC ATTCAGCAAG 
ACCAGAGAGG AAAAAATGGA GGACATGTTT GCACAGAAAG ATATGGAGGA ATCAGAGCTT 
CGGGGCTGGA TAGAGAAGTT ACAAGTGCGT CTGCAGACGA GTGTGATTGA CTCTTCACAA 
CAACTACAGG CTGTTCTGGA GTCAGTGGTG GTCAAAAAAC AGGGCTTGTG TGAAACCCTG 
CAGTCCTGGA ACAACAGACT TCAAGAACTG TTCCAGCAAG AGAAAGGCAG AAAACGTCTA 
TCTGTTCCTC CTAGCCCAGG CAGACACAGA CAACCTACAA CAGATGAGAG CAAGACTAGT 
GCTTTGGAGT CTTCTCCTCG TAACCCTTCC CCAGTGGTCC CAAATGGAGA CAAAGAGGAC 
CGTCATCTCA ATACATTTCC ATCAAGTTCA GGGTCCTCGT CGTTACTACA GTTACCATCT 
CCAGCTGAAC AACCTTCAGA TGTCATTACA AGCGGACCAT CTTTCCCTGA TCAGGACTCT 
GTTAGCAATC CAGAGGACAT GTTTGATGGA CACTTGCTTG GTTCCAATGA TAGTCAGGTA 
AAGGAAAAGT CCACCATGAA AACAATCCTG GCCAACCTGT TACCTGGAAA CAGCTACAAT 
CCCATCCCTT TTCCTTTTGA CCCAGACAAG CACTATCTGA TGTATGAGCA TGAGAGAGTT 
CCTATAGCCG TGTGTGAGAG AGAGCCCAGC TCCATAATTG CCTTTGCACT CAGCTGCAAA 
GAGTATAAAA CTGCCCTTGA AGAGCTCACA AAGACAACAG CAAAGACTGG AGGTGATGAC 
ACTTCTCAGA CCATCAGTGC TGGAGAGAGC AGAGTGAAGA ACAGTCCAGC CAAACCCAGT 
GACAACAGCA TGTCACAGCT GAGCCGCAGC AGCGTCGACG CTGATCCACT CAAGGAGCCT 
GAAAGTGCAG ACAAACAGAA GAAGCAGACA GGGAACCCAC ATATTGAGCT GCAGTTCTCC 
GATGCAAATG CTAAGTTCTA CTGCCGGATC TACTATGCTG AAGAGTTCCA TAAAATGCGG 
GAGGAGATCA TGGAGAGCAC AGAGGATGAT TTTGTGCGAT CTCTTTCCCA CTGTGTCAAC 
TGGCAGGCGC GTGGTGGGAA ATCTGGTGCA GTTTTCTATG CCACTGAAGA TGATCGGTTT 
ATTCTGAAGC AGATGCCCAG ATTAGAGGTC CAGTCCTTTT TAGACTTTGC ACCCCACTAC 
TTTACGTACA TCACAGGAGC GGTTCACCTA AAGCGACCCA CAGCACTTGC TAAGATTCTG 
GGAGTGTACC GCATCGGCTA CAAGAACTCC CAGAACAACA CTGAGAAGAA GCTTGACCTG 
CTTGTGATGG AAAACCTTTT CTATGGACGA AAGATGGCAC AGGTGTTTGA CCTGAAAGGA 
TCGCTGAGAA ACAGGAACGT GAAGACGGAC CAGGGAAAGG AGAGCTGTGA GGTGGTGCTC 
CTAGATGAGA ACCTGCTCAA ACTGGTGCAT GACAATCCCC TTTATATTCG CTCGCACTGC 
AAGGCCATCC TGCGTGCTGC CATCCACAGC GACGCGCTCT TCCTGTCCAG CCACCTCATC 
ATTGATTACT CCCTGCTGGT GGGCCGAGAC GATTCCACAG ATGAGCTAGT TGTGGGGATC 
ATAGATTATA TCCGGACTTT CACTTGGGAT AAAAAGCTGG AGATGGTGGT CAAATCTACT 
GGGATTCTTG GAGGTCAAGG TAAAATGCCC ACAGTGGTGT CACCAGAGCT GTATCGAACA 
CGTTTCTGTG AGGCCATGGA TAAATACTTC CTTATGGTCC CTGATCACTG GAGTGGACTT 
GGGCTCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167726.1
CDS	164..6418
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQRKNSIGMRKNHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167669.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167669.4

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG AAAAAATTCC 
ATTGGGATGA GGAAGAATCA TCAGGAATCT CAGAACAGCG GTCTCAATTC CAGACTGACA 
GTGGTCCAAG AGGATATTGG AAAGTCACCT GCCAGGAAGA GGTCAGCTAG TGTGACCAAT 
TTGTCTTTGG ACCGTTCCGG CTCATCCATG GTTCCTGCCT ATGAGAGTTC GGTTAGTCCA 
CAGAACAGCC GAGCTCTACC CAAGACCGAC CACAGCGAAG AAGAGCGAAA GATCCTTCTA 
GATTCTTTTC AACTTAAAGA TATATGGAAG AAGATTTGCC ACAACACTAC TGGGATGGAG 
TTCCAGGACC ATCGGTACTG GCTGCGCACT TACCCCAACT GCATTGTGGG TAAAGAGTTG 
GTCAACTGGC TTTTAAGAAA TGGCACTATT TCCACTAGGG CTCAAGCCAT AGCTATAGGA 
CAGGCTTTGG TAGATGGCCG CTGGCTTGAC TGTGTAACCC ATCATGATCA GATCTTCCGT 
GATGAGTACG CCCTTTATCG CCCCCTTCAG AGCACAGAGT TCTCCGAAAC TCCTTCTCCA 
GACAGTGACA GTGTTAATTC CCTGGAGGGA CACTCTGAAC CGTCATGGTT TAAAGACATC 
AAGTTTGATG ACAGTGACAC CGAGCAGCTA GCAGATGAAA ATGACTATGT CACACCAAGC 
TCGTCCAGCC CCAGCAAAAG AACATCTGTC AGCAGTTTCC ACTCTGCAGT AGACAGTGAC 
TCGGCCGCCT CCATCAGTCT AAACATGGAG CAGGACAACG TCAATTTCCA CATTAAGAAG 
CAGGCCAAGT ACCCCCATGT GCCTCCCTAC CCAGCTGAGC AAAAAATGGA AGTCTTGCTC 
TCCGAAGATG GAGGTCAGCA TATATCCATC AGTGATGCAT TCATCAAAGA GTCTTTGTTC 
AACCGGAGGG TTGAGGAGAA AGCTAAAGAG ATGCTCTTCA CTCCTCTCGG CTGGCATCAC 
AGCTCTCTGG ATCAGCTGCG GGAAGAGAAT GGGGAAAAGA AAGCGATGGA GCGGTTGCTG 
TCAGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAAC TGCTGTACAG TGAATCACTG 
TCTCTGTCGT GGCGTGACAT CATCGTACCT GTGGTGAGGC AGGTGGTGCA GACGGTGCGA 
CCGGATGTGC GCAGCTGTGA CGATGATATG GACATCCGTC AGTTTGTCCA CATCAAGAAG 
ATTCCAGGAG GAAAGAAGTT TGACTCTGCT GTAGTGAATG GTTTTGTTTG CACAAAGAAT 
ATCGCACACA AAAAGATGAA CTCTTACATC AAAAACCCCA AGATCCTTCT TCTCAAATGC 
TCCATTGAGT ATCTATATAG AGAGGAGACC AAGTTCACCT GCATTGACCC AATTGTTTTA 
CAGGAGCGTG AGTTTCTTAA GAACTATGTT CAGAGGATTG TTGATGTCCG ACCAAACTTA 
GTCCTGGTGG AGAAGACTGT ATCGCGAATT GCTCAGGACA TGCTACTAGA GCACGGCATT 
GCCATTGTGA TTAATGTCAA ACCTCAAGTA CTGGACCGTG TGAGTCGAAT GACTCAGGGA 
GATTTAGTCA TTTCAATGGA TCAGCTTCTT ACAAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACTTGC ACTCCTTCCA GCTACCAAAT AGTGAAGTGA AAACCCTGAT GTTTTTTGAG 
GGCTGTCCTC CTCAGCTGGG CTGCACTATT AAGCTCCGCG GAGCCTCAGA GTATGAGCTG 
GCACGGGTAA AAGAGATCAT CATTTTTATG GTGTGTGTGG CATACCACTC GCAGCTAGAG 
ATCTCTTTCC TCATGGATGA GTTCGCTATG CCTCCCAGTC TGGCTGAGAG TTCTTCTTTC 
CCATGTCTGC TGGAAAGCAC CACTTTACGG GAAGAGGATG AAACTAGTGG GGAACGGGAA 
AGCCAAGACA ATGATGAGTC TACACTGGGA GATGACAATC TCACACTTGT TCCAGGAGAT 
GACTTTGAGA CAGGATTTAC AGGGACTATC AAAGCCCACA GTCGAGAGCC TTCCACCTCT 
GAATCTTCTC ATAAAGATGG AGAAAGCCCC AGAATAAATA AAAATGCTTC AATTACCTCC 
TTCTCTGGAG GGGAGGAAGA AAATATAAAG ACCTCCACGC CACTTTCCTC CTTCTCTTCC 
ACCTTTCCTC AGCCGGTGAC ACCACCTTTC CTGATATCAG ACGTAAAAGA AATGTCACAG 
GAAGTGGTCA AGGGGTCAGG TGAAGGGGAG AAGAACAAAT ATCTGGAGTC GACTCTGGTC 
CATCAGGACA GCACAAGCTC TGAGACCTCC CTTCCCCCAA CTCGGCTCTT TAGAGACCCC 
TTACAGGACG ACACAGGCCT GTTTGTGACA GAGCATGTAG CTTCTTCAGA TGACCGCCTC 
AAATCCATCT CTGCTTTATT CAAACAGGAG CTAAAAGACA TCATCCTGTG CATTTCACCC 
TTTATTACCT TTCGAGAGCC TTTCTTGCTT ACCCCAGCTG GACTGCGGTG TCCTAGCCGG 
GATTATTTCC CTGAACAGGT TTACCTTTCA CCTCTGCTGA ACAAGGATTC AAAAGAACTG 
GATGGACGCC GCAAGAGGCA GCTGCTGAAA GAGTCCAGCC CAGGTTCTGG TAGCCTGACC 
AATGGTTCCG TTTCACACCA GAGAGCCATT CAGATCTTGC CCTGTCACAA ACTCACTAGT 
GCCCGAATAG CAGAACAGGT AGGCAGCAGT CAGCAGCTGG CACGCATGTT AGCGGACTAC 
CGTGCCCAAG GAGGACGTAT CCGGCAAAGA GAGGGAACAC AATTTCGTGA AGCCCCTCCC 
CCAAAGGCGC CAATAAAGGC AGATAGTGAA GAAGATAAAG GTGCAGGAGT GATTGACATG 
ACCTGGTCTA CTAAGTTGGA CTGTTTAAAT CCAATCAACC ATCAGAGGCT CTGTGTTCTG 
TTCAGTAGCT CTTCAGCACA GTCTAATAAT GCCCCAAACC CCTGCGTCAG TCCCTGGATT 
GTTACAATGG AGTTTTATGG AAAGAATGAC TTGACTCTTG GTGTGTTTCT GGAGAGATAC 
TGTTTTAGAC CATCTTACCA GTGCCCCAGC ATGTACTGTG AGACCCCCAT GGTTCACCAC 
ATCCGGCGCT TTGTGCACGG TAAAGGCTGC GTCCAGATTG TACTGAAAGA GCTGGACTCG 
CCTGTTCCTG GATATCAGCA CACAATCCTC AACTACTCAT GGTGTCGCAT CTGCAAACAG 
GTGACTCCTG TGGTCCCTTT GTCTAATGAC TCTTGGTCCA TGTCCTTCGC CAAGTACCTT 
GAGCTTCGTT TCTATGGTCA TCAGTACACC CGTCGGGCTA ACGCTGAGCC TTGTGGCCAC 
TCTATCCATA AAGATTATCA CCAGTACTTC TCTTACAACC AGATGGTGGC ATCCTTCAGC 
TACATTCCAG TGAGGCTGTT AGAGATCTGT CTGCCTCCCC CGAAGATCAT CATCAGGAAC 
CAAGGTCCAT CGAAATCCAG CTTGCAGCAG GATCTCAAAG ACTTCTCCCA GAAGGTGGCT 
CAGGTGTACC TGGCTATAGA TGACCGTCTT ACCTCCTTAA AAACGGACAC ATTCAGCAAG 
ACCAGAGAGG AAAAAATGGA GGACATGTTT GCACAGAAAG ATATGGAGGA ATCAGAGCTT 
CGGGGCTGGA TAGAGAAGTT ACAAGTGCGT CTGCAGACGA GTGTGATTGA CTCTTCACAA 
CAACTACAGG CTGTTCTGGA GTCAGTGGTG GTCAAAAAAC AGGGCTTGTG TGAAACCCTG 
CAGTCCTGGA ACAACAGACT TCAAGAACTG TTCCAGCAAG AGAAAGGCAG AAAACGTCTA 
TCTGTTCCTC CTAGCCCAGG CAGACACAGA CAACCTACAA CAGATGAGAG CAAGACTAGT 
GCTTTGGAGT CTTCTCCTCG TAACCCTTCC CCAGTGGTCC CAAATGGAGA CAAAGAGGAC 
CGTCATCTCA ATACATTTCC ATCAAGTTCA GGGTCCTCGT CGTTACTACA GTTACCATCT 
CCAGCTGAAC AACCTTCAGA TGTCATTACA AGCGGACCAT CTTTCCCTGA TCAGGACTCT 
GTTAGCAATC CAGAGGACAT GTTTGATGGA CACTTGCTTG GTTCCAATGA TAGTCAGGTA 
AAGGAAAAGT CCACCATGAA AACAATCCTG GCCAACCTGT TACCTGGAAA CAGCTACAAT 
CCCATCCCTT TTCCTTTTGA CCCAGACAAG CACTATCTGA TGTATGAGCA TGAGAGAGTT 
CCTATAGCCG TGTGTGAGAG AGAGCCCAGC TCCATAATTG CCTTTGCACT CAGCTGCAAA 
GAGTATAAAA CTGCCCTTGA AGAGCTCACA AAGACAACAG CAAAGACTGG AGGTGATGAC 
ACTTCTCAGA CCATCAGTGC TGGAGAGAGC AGAGTGAAGA ACAGTCCAGC CAAACCCAGT 
GACAACAGCA TGTCACAGCT GAGCCGCAGC AGCGTCGACG CTGATCCACT CAAGGAGCCT 
GAAAGTGCAG ACAAACAGAA GAAGCAGACA GGGAACCCAC ATATTGAGCT GCAGTTCTCC 
GATGCAAATG CTAAGTTCTA CTGCCGGATC TACTATGCTG AAGAGTTCCA TAAAATGCGG 
GAGGAGATCA TGGAGAGCAC AGAGGATGAT TTTGTGCGAT CTCTTTCCCA CTGTGTCAAC 
TGGCAGGCGC GTGGTGGGAA ATCTGGTGCA GTTTTCTATG CCACTGAAGA TGATCGGTTT 
ATTCTGAAGC AGATGCCCAG ATTAGAGGTC CAGTCCTTTT TAGACTTTGC ACCCCACTAC 
TTTACGTACA TCACAGGAGC GGTTCACCTA AAGCGACCCA CAGCACTTGC TAAGATTCTG 
GGAGTGTACC GCATCGGCTA CAAGAACTCC CAGAACAACA CTGAGAAGAA GCTTGACCTG 
CTTGTGATGG AAAACCTTTT CTATGGACGA AAGATGGCAC AGGTGTTTGA CCTGAAAGGA 
TCGCTGAGAA ACAGGAACGT GAAGACGGAC CAGGGAAAGG AGAGCTGTGA GGTGGTGCTC 
CTAGATGAGA ACCTGCTCAA ACTGGTGCAT GACAATCCCC TTTATATTCG CTCGCACTGC 
AAGGCCATCC TGCGTGCTGC CATCCACAGC GACGCGCTCT TCCTGTCCAG CCACCTCATC 
ATTGATTACT CCCTGCTGGT GGGCCGAGAC GATTCCACAG ATGAGCTAGT TGTGGGGATC 
ATAGATTATA TCCGGACTTT CACTTGGGAT AAAAAGCTGG AGATGGTGGT CAAATCTACT 
GGGATTCTTG GAGGTCAAGG TAAAATGCCC ACAGTGGTGT CACCAGAGCT GTATCGAACA 
CGTTTCTGTG AGGCCATGGA TAAATACTTC CTTATGGTCC CTGATCACTG GAGTGGACTT 
GGGCTCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167670.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167727.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X10, mRNA.

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CloneID	ODa15220
Clone ID Related Accession (Same CDS sequence)	XM_005167670.3 , XM_005167670.4
Accession Version	XM_005167670.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAT GGAAGTCTTG CTCTCCGAAG ATGGAGGTCA GCATATATCC 
ATCAGTGATG CATTCATCAA AGAGTCTTTG TTCAACCGGA GGGTTGAGGA GAAAGCTAAA 
GAGATGCTCT TCACTCCTCT CGGCTGGCAT CACAGCTCTC TGGATCAGCT GCGGGAAGAG 
AATGGGGAAA AGAAAGCGAT GGAGCGGTTG CTGTCAGCAA ATCACAGCCA CATGATGGCG 
CTGCTGCAGC AACTGCTGTA CAGTGAATCA CTGTCTCTGT CGTGGCGTGA CATCATCGTA 
CCTGTGGTGA GGCAGGTGGT GCAGACGGTG CGACCGGATG TGCGCAGCTG TGACGATGAT 
ATGGACATCC GTCAGTTTGT CCACATCAAG AAGATTCCAG GAGGAAAGAA GTTTGACTCT 
GCTGTAGTGA ATGGTTTTGT TTGCACAAAG AATATCGCAC ACAAAAAGAT GAACTCTTAC 
ATCAAAAACC CCAAGATCCT TCTTCTCAAA TGCTCCATTG AGTATCTATA TAGAGAGGAG 
ACCAAGTTCA CCTGCATTGA CCCAATTGTT TTACAGGAGC GTGAGTTTCT TAAGAACTAT 
GTTCAGAGGA TTGTTGATGT CCGACCAAAC TTAGTCCTGG TGGAGAAGAC TGTATCGCGA 
ATTGCTCAGG ACATGCTACT AGAGCACGGC ATTGCCATTG TGATTAATGT CAAACCTCAA 
GTACTGGACC GTGTGAGTCG AATGACTCAG GGAGATTTAG TCATTTCAAT GGATCAGCTT 
CTTACAAAAC CTCGTCTGGG TACCTGCCAC AAGTTTTACT TGCACTCCTT CCAGCTACCA 
AATAGTGAAG TGAAAACCCT GATGTTTTTT GAGGGCTGTC CTCCTCAGCT GGGCTGCACT 
ATTAAGCTCC GCGGAGCCTC AGAGTATGAG CTGGCACGGG TAAAAGAGAT CATCATTTTT 
ATGGTGTGTG TGGCATACCA CTCGCAGCTA GAGATCTCTT TCCTCATGGA TGAGTTCGCT 
ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT TTCCCATGTC TGCTGGAAAG CACCACTTTA 
CGGGAAGAGG ATGAAACTAG TGGGGAACGG GAAAGCCAAG ACAATGATGA GTCTACACTG 
GGAGATGACA ATCTCACACT TGTTCCAGGA GATGACTTTG AGACAGGATT TACAGGGACT 
ATCAAAGCCC ACAGTCGAGA GCCTTCCACC TCTGAATCTT CTCATAAAGA TGGAGAAAGC 
CCCAGAATAA ATAAAAATGC TTCAATTACC TCCTTCTCTG GAGGGGAGGA AGAAAATATA 
AAGACCTCCA CGCCACTTTC CTCCTTCTCT TCCACCTTTC CTCAGCCGGT GACACCACCT 
TTCCTGATAT CAGACGTAAA AGAAATGTCA CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG 
GAGAAGAACA AATATCTGGA GTCGACTCTG GTCCATCAGG ACAGCACAAG CTCTGAGACC 
TCCCTTCCCC CAACTCGGCT CTTTAGAGAC CCCTTACAGG ACGACACAGG CCTGTTTGTG 
ACAGAGCATG TAGCTTCTTC AGATGACCGC CTCAAATCCA TCTCTGCTTT ATTCAAACAG 
GAGCTAAAAG ACATCATCCT GTGCATTTCA CCCTTTATTA CCTTTCGAGA GCCTTTCTTG 
CTTACCCCAG CTGGACTGCG GTGTCCTAGC CGGGATTATT TCCCTGAACA GGTTTACCTT 
TCACCTCTGC TGAACAAGGA TTCAAAAGAA CTGGATGGAC GCCGCAAGAG GCAGCTGCTG 
AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG ACCAATGGTT CCGTTTCACA CCAGAGAGCC 
ATTCAGATCT TGCCCTGTCA CAAACTCACT AGTGCCCGAA TAGCAGAACA GGTAGGCAGC 
AGTCAGCAGC TGGCACGCAT GTTAGCGGAC TACCGTGCCC AAGGAGGACG TATCCGGCAA 
AGAGAGGGAA CACAATTTCG TGAAGCCCCT CCCCCAAAGG CGCCAATAAA GGCAGATAGT 
GAAGAAGATA AAGGTGCAGG AGTGATTGAC ATGACCTGGT CTACTAAGTT GGACTGTTTA 
AATCCAATCA ACCATCAGAG GCTCTGTGTT CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT 
AATGCCCCAA ACCCCTGCGT CAGTCCCTGG ATTGTTACAA TGGAGTTTTA TGGAAAGAAT 
GACTTGACTC TTGGTGTGTT TCTGGAGAGA TACTGTTTTA GACCATCTTA CCAGTGCCCC 
AGCATGTACT GTGAGACCCC CATGGTTCAC CACATCCGGC GCTTTGTGCA CGGTAAAGGC 
TGCGTCCAGA TTGTACTGAA AGAGCTGGAC TCGCCTGTTC CTGGATATCA GCACACAATC 
CTCAACTACT CATGGTGTCG CATCTGCAAA CAGGTGACTC CTGTGGTCCC TTTGTCTAAT 
GACTCTTGGT CCATGTCCTT CGCCAAGTAC CTTGAGCTTC GTTTCTATGG TCATCAGTAC 
ACCCGTCGGG CTAACGCTGA GCCTTGTGGC CACTCTATCC ATAAAGATTA TCACCAGTAC 
TTCTCTTACA ACCAGATGGT GGCATCCTTC AGCTACATTC CAGTGAGGCT GTTAGAGATC 
TGTCTGCCTC CCCCGAAGAT CATCATCAGG AACCAAGGTC CATCGAAATC CAGCTTGCAG 
CAGGATCTCA AAGACTTCTC CCAGAAGGTG GCTCAGGTGT ACCTGGCTAT AGATGACCGT 
CTTACCTCCT TAAAAACGGA CACATTCAGC AAGACCAGAG AGGAAAAAAT GGAGGACATG 
TTTGCACAGA AAGATATGGA GGAATCAGAG CTTCGGGGCT GGATAGAGAA GTTACAAGTG 
CGTCTGCAGA CGAGTGTGAT TGACTCTTCA CAACAACTAC AGGCTGTTCT GGAGTCAGTG 
GTGGTCAAAA AACAGGGCTT GTGTGAAACC CTGCAGTCCT GGAACAACAG ACTTCAAGAA 
CTGTTCCAGC AAGAGAAAGG CAGAAAACGT CTATCTGTTC CTCCTAGCCC AGGCAGACAC 
AGACAACCTA CAACAGATGA GAGCAAGACT AGTGCTTTGG AGTCTTCTCC TCGTAACCCT 
TCCCCAGTGG TCCCAAATGG AGACAAAGAG GACCGTCATC TCAATACATT TCCATCAAGT 
TCAGGGTCCT CGTCGTTACT ACAGTTACCA TCTCCAGCTG AACAACCTTC AGATGTCATT 
ACAAGCGGAC CATCTTTCCC TGATCAGGAC TCTGTTAGCA ATCCAGAGGA CATGTTTGAT 
GGACACTTGC TTGGTTCCAA TGATAGTCAG GTAAAGGAAA AGTCCACCAT GAAAACAATC 
CTGGCCAACC TGTTACCTGG AAACAGCTAC AATCCCATCC CTTTTCCTTT TGACCCAGAC 
AAGCACTATC TGATGTATGA GCATGAGAGA GTTCCTATAG CCGTGTGTGA GAGAGAGCCC 
AGCTCCATAA TTGCCTTTGC ACTCAGCTGC AAAGAGTATA AAACTGCCCT TGAAGAGCTC 
ACAAAGACAA CAGCAAAGAC TGGAGGTGAT GACACTTCTC AGACCATCAG TGCTGGAGAG 
AGCAGAGTGA AGAACAGTCC AGCCAAACCC AGTGACAACA GCATGTCACA GCTGAGCCGC 
AGCAGCGTCG ACGCTGATCC ACTCAAGGAG CCTGAAAGTG CAGACAAACA GAAGAAGCAG 
ACAGGGAACC CACATATTGA GCTGCAGTTC TCCGATGCAA ATGCTAAGTT CTACTGCCGG 
ATCTACTATG CTGAAGAGTT CCATAAAATG CGGGAGGAGA TCATGGAGAG CACAGAGGAT 
GATTTTGTGC GATCTCTTTC CCACTGTGTC AACTGGCAGG CGCGTGGTGG GAAATCTGGT 
GCAGTTTTCT ATGCCACTGA AGATGATCGG TTTATTCTGA AGCAGATGCC CAGATTAGAG 
GTCCAGTCCT TTTTAGACTT TGCACCCCAC TACTTTACGT ACATCACAGG AGCGGTTCAC 
CTAAAGCGAC CCACAGCACT TGCTAAGATT CTGGGAGTGT ACCGCATCGG CTACAAGAAC 
TCCCAGAACA ACACTGAGAA GAAGCTTGAC CTGCTTGTGA TGGAAAACCT TTTCTATGGA 
CGAAAGATGG CACAGGTGTT TGACCTGAAA GGATCGCTGA GAAACAGGAA CGTGAAGACG 
GACCAGGGAA AGGAGAGCTG TGAGGTGGTG CTCCTAGATG AGAACCTGCT CAAACTGGTG 
CATGACAATC CCCTTTATAT TCGCTCGCAC TGCAAGGCCA TCCTGCGTGC TGCCATCCAC 
AGCGACGCGC TCTTCCTGTC CAGCCACCTC ATCATTGATT ACTCCCTGCT GGTGGGCCGA 
GACGATTCCA CAGATGAGCT AGTTGTGGGG ATCATAGATT ATATCCGGAC TTTCACTTGG 
GATAAAAAGC TGGAGATGGT GGTCAAATCT ACTGGGATTC TTGGAGGTCA AGGTAAAATG 
CCCACAGTGG TGTCACCAGA GCTGTATCGA ACACGTTTCT GTGAGGCCAT GGATAAATAC 
TTCCTTATGG TCCCTGATCA CTGGAGTGGA CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167727.1
CDS	155..6382
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQSHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167670.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167670.3

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAT GGAAGTCTTG CTCTCCGAAG ATGGAGGTCA GCATATATCC 
ATCAGTGATG CATTCATCAA AGAGTCTTTG TTCAACCGGA GGGTTGAGGA GAAAGCTAAA 
GAGATGCTCT TCACTCCTCT CGGCTGGCAT CACAGCTCTC TGGATCAGCT GCGGGAAGAG 
AATGGGGAAA AGAAAGCGAT GGAGCGGTTG CTGTCAGCAA ATCACAGCCA CATGATGGCG 
CTGCTGCAGC AACTGCTGTA CAGTGAATCA CTGTCTCTGT CGTGGCGTGA CATCATCGTA 
CCTGTGGTGA GGCAGGTGGT GCAGACGGTG CGACCGGATG TGCGCAGCTG TGACGATGAT 
ATGGACATCC GTCAGTTTGT CCACATCAAG AAGATTCCAG GAGGAAAGAA GTTTGACTCT 
GCTGTAGTGA ATGGTTTTGT TTGCACAAAG AATATCGCAC ACAAAAAGAT GAACTCTTAC 
ATCAAAAACC CCAAGATCCT TCTTCTCAAA TGCTCCATTG AGTATCTATA TAGAGAGGAG 
ACCAAGTTCA CCTGCATTGA CCCAATTGTT TTACAGGAGC GTGAGTTTCT TAAGAACTAT 
GTTCAGAGGA TTGTTGATGT CCGACCAAAC TTAGTCCTGG TGGAGAAGAC TGTATCGCGA 
ATTGCTCAGG ACATGCTACT AGAGCACGGC ATTGCCATTG TGATTAATGT CAAACCTCAA 
GTACTGGACC GTGTGAGTCG AATGACTCAG GGAGATTTAG TCATTTCAAT GGATCAGCTT 
CTTACAAAAC CTCGTCTGGG TACCTGCCAC AAGTTTTACT TGCACTCCTT CCAGCTACCA 
AATAGTGAAG TGAAAACCCT GATGTTTTTT GAGGGCTGTC CTCCTCAGCT GGGCTGCACT 
ATTAAGCTCC GCGGAGCCTC AGAGTATGAG CTGGCACGGG TAAAAGAGAT CATCATTTTT 
ATGGTGTGTG TGGCATACCA CTCGCAGCTA GAGATCTCTT TCCTCATGGA TGAGTTCGCT 
ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT TTCCCATGTC TGCTGGAAAG CACCACTTTA 
CGGGAAGAGG ATGAAACTAG TGGGGAACGG GAAAGCCAAG ACAATGATGA GTCTACACTG 
GGAGATGACA ATCTCACACT TGTTCCAGGA GATGACTTTG AGACAGGATT TACAGGGACT 
ATCAAAGCCC ACAGTCGAGA GCCTTCCACC TCTGAATCTT CTCATAAAGA TGGAGAAAGC 
CCCAGAATAA ATAAAAATGC TTCAATTACC TCCTTCTCTG GAGGGGAGGA AGAAAATATA 
AAGACCTCCA CGCCACTTTC CTCCTTCTCT TCCACCTTTC CTCAGCCGGT GACACCACCT 
TTCCTGATAT CAGACGTAAA AGAAATGTCA CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG 
GAGAAGAACA AATATCTGGA GTCGACTCTG GTCCATCAGG ACAGCACAAG CTCTGAGACC 
TCCCTTCCCC CAACTCGGCT CTTTAGAGAC CCCTTACAGG ACGACACAGG CCTGTTTGTG 
ACAGAGCATG TAGCTTCTTC AGATGACCGC CTCAAATCCA TCTCTGCTTT ATTCAAACAG 
GAGCTAAAAG ACATCATCCT GTGCATTTCA CCCTTTATTA CCTTTCGAGA GCCTTTCTTG 
CTTACCCCAG CTGGACTGCG GTGTCCTAGC CGGGATTATT TCCCTGAACA GGTTTACCTT 
TCACCTCTGC TGAACAAGGA TTCAAAAGAA CTGGATGGAC GCCGCAAGAG GCAGCTGCTG 
AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG ACCAATGGTT CCGTTTCACA CCAGAGAGCC 
ATTCAGATCT TGCCCTGTCA CAAACTCACT AGTGCCCGAA TAGCAGAACA GGTAGGCAGC 
AGTCAGCAGC TGGCACGCAT GTTAGCGGAC TACCGTGCCC AAGGAGGACG TATCCGGCAA 
AGAGAGGGAA CACAATTTCG TGAAGCCCCT CCCCCAAAGG CGCCAATAAA GGCAGATAGT 
GAAGAAGATA AAGGTGCAGG AGTGATTGAC ATGACCTGGT CTACTAAGTT GGACTGTTTA 
AATCCAATCA ACCATCAGAG GCTCTGTGTT CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT 
AATGCCCCAA ACCCCTGCGT CAGTCCCTGG ATTGTTACAA TGGAGTTTTA TGGAAAGAAT 
GACTTGACTC TTGGTGTGTT TCTGGAGAGA TACTGTTTTA GACCATCTTA CCAGTGCCCC 
AGCATGTACT GTGAGACCCC CATGGTTCAC CACATCCGGC GCTTTGTGCA CGGTAAAGGC 
TGCGTCCAGA TTGTACTGAA AGAGCTGGAC TCGCCTGTTC CTGGATATCA GCACACAATC 
CTCAACTACT CATGGTGTCG CATCTGCAAA CAGGTGACTC CTGTGGTCCC TTTGTCTAAT 
GACTCTTGGT CCATGTCCTT CGCCAAGTAC CTTGAGCTTC GTTTCTATGG TCATCAGTAC 
ACCCGTCGGG CTAACGCTGA GCCTTGTGGC CACTCTATCC ATAAAGATTA TCACCAGTAC 
TTCTCTTACA ACCAGATGGT GGCATCCTTC AGCTACATTC CAGTGAGGCT GTTAGAGATC 
TGTCTGCCTC CCCCGAAGAT CATCATCAGG AACCAAGGTC CATCGAAATC CAGCTTGCAG 
CAGGATCTCA AAGACTTCTC CCAGAAGGTG GCTCAGGTGT ACCTGGCTAT AGATGACCGT 
CTTACCTCCT TAAAAACGGA CACATTCAGC AAGACCAGAG AGGAAAAAAT GGAGGACATG 
TTTGCACAGA AAGATATGGA GGAATCAGAG CTTCGGGGCT GGATAGAGAA GTTACAAGTG 
CGTCTGCAGA CGAGTGTGAT TGACTCTTCA CAACAACTAC AGGCTGTTCT GGAGTCAGTG 
GTGGTCAAAA AACAGGGCTT GTGTGAAACC CTGCAGTCCT GGAACAACAG ACTTCAAGAA 
CTGTTCCAGC AAGAGAAAGG CAGAAAACGT CTATCTGTTC CTCCTAGCCC AGGCAGACAC 
AGACAACCTA CAACAGATGA GAGCAAGACT AGTGCTTTGG AGTCTTCTCC TCGTAACCCT 
TCCCCAGTGG TCCCAAATGG AGACAAAGAG GACCGTCATC TCAATACATT TCCATCAAGT 
TCAGGGTCCT CGTCGTTACT ACAGTTACCA TCTCCAGCTG AACAACCTTC AGATGTCATT 
ACAAGCGGAC CATCTTTCCC TGATCAGGAC TCTGTTAGCA ATCCAGAGGA CATGTTTGAT 
GGACACTTGC TTGGTTCCAA TGATAGTCAG GTAAAGGAAA AGTCCACCAT GAAAACAATC 
CTGGCCAACC TGTTACCTGG AAACAGCTAC AATCCCATCC CTTTTCCTTT TGACCCAGAC 
AAGCACTATC TGATGTATGA GCATGAGAGA GTTCCTATAG CCGTGTGTGA GAGAGAGCCC 
AGCTCCATAA TTGCCTTTGC ACTCAGCTGC AAAGAGTATA AAACTGCCCT TGAAGAGCTC 
ACAAAGACAA CAGCAAAGAC TGGAGGTGAT GACACTTCTC AGACCATCAG TGCTGGAGAG 
AGCAGAGTGA AGAACAGTCC AGCCAAACCC AGTGACAACA GCATGTCACA GCTGAGCCGC 
AGCAGCGTCG ACGCTGATCC ACTCAAGGAG CCTGAAAGTG CAGACAAACA GAAGAAGCAG 
ACAGGGAACC CACATATTGA GCTGCAGTTC TCCGATGCAA ATGCTAAGTT CTACTGCCGG 
ATCTACTATG CTGAAGAGTT CCATAAAATG CGGGAGGAGA TCATGGAGAG CACAGAGGAT 
GATTTTGTGC GATCTCTTTC CCACTGTGTC AACTGGCAGG CGCGTGGTGG GAAATCTGGT 
GCAGTTTTCT ATGCCACTGA AGATGATCGG TTTATTCTGA AGCAGATGCC CAGATTAGAG 
GTCCAGTCCT TTTTAGACTT TGCACCCCAC TACTTTACGT ACATCACAGG AGCGGTTCAC 
CTAAAGCGAC CCACAGCACT TGCTAAGATT CTGGGAGTGT ACCGCATCGG CTACAAGAAC 
TCCCAGAACA ACACTGAGAA GAAGCTTGAC CTGCTTGTGA TGGAAAACCT TTTCTATGGA 
CGAAAGATGG CACAGGTGTT TGACCTGAAA GGATCGCTGA GAAACAGGAA CGTGAAGACG 
GACCAGGGAA AGGAGAGCTG TGAGGTGGTG CTCCTAGATG AGAACCTGCT CAAACTGGTG 
CATGACAATC CCCTTTATAT TCGCTCGCAC TGCAAGGCCA TCCTGCGTGC TGCCATCCAC 
AGCGACGCGC TCTTCCTGTC CAGCCACCTC ATCATTGATT ACTCCCTGCT GGTGGGCCGA 
GACGATTCCA CAGATGAGCT AGTTGTGGGG ATCATAGATT ATATCCGGAC TTTCACTTGG 
GATAAAAAGC TGGAGATGGT GGTCAAATCT ACTGGGATTC TTGGAGGTCA AGGTAAAATG 
CCCACAGTGG TGTCACCAGA GCTGTATCGA ACACGTTTCT GTGAGGCCAT GGATAAATAC 
TTCCTTATGG TCCCTGATCA CTGGAGTGGA CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_005167670.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167727.1 Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X10, mRNA.

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CloneID	ODa15220
Clone ID Related Accession (Same CDS sequence)	XM_005167670.3 , XM_005167670.4
Accession Version	XM_005167670.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAT GGAAGTCTTG CTCTCCGAAG ATGGAGGTCA GCATATATCC 
ATCAGTGATG CATTCATCAA AGAGTCTTTG TTCAACCGGA GGGTTGAGGA GAAAGCTAAA 
GAGATGCTCT TCACTCCTCT CGGCTGGCAT CACAGCTCTC TGGATCAGCT GCGGGAAGAG 
AATGGGGAAA AGAAAGCGAT GGAGCGGTTG CTGTCAGCAA ATCACAGCCA CATGATGGCG 
CTGCTGCAGC AACTGCTGTA CAGTGAATCA CTGTCTCTGT CGTGGCGTGA CATCATCGTA 
CCTGTGGTGA GGCAGGTGGT GCAGACGGTG CGACCGGATG TGCGCAGCTG TGACGATGAT 
ATGGACATCC GTCAGTTTGT CCACATCAAG AAGATTCCAG GAGGAAAGAA GTTTGACTCT 
GCTGTAGTGA ATGGTTTTGT TTGCACAAAG AATATCGCAC ACAAAAAGAT GAACTCTTAC 
ATCAAAAACC CCAAGATCCT TCTTCTCAAA TGCTCCATTG AGTATCTATA TAGAGAGGAG 
ACCAAGTTCA CCTGCATTGA CCCAATTGTT TTACAGGAGC GTGAGTTTCT TAAGAACTAT 
GTTCAGAGGA TTGTTGATGT CCGACCAAAC TTAGTCCTGG TGGAGAAGAC TGTATCGCGA 
ATTGCTCAGG ACATGCTACT AGAGCACGGC ATTGCCATTG TGATTAATGT CAAACCTCAA 
GTACTGGACC GTGTGAGTCG AATGACTCAG GGAGATTTAG TCATTTCAAT GGATCAGCTT 
CTTACAAAAC CTCGTCTGGG TACCTGCCAC AAGTTTTACT TGCACTCCTT CCAGCTACCA 
AATAGTGAAG TGAAAACCCT GATGTTTTTT GAGGGCTGTC CTCCTCAGCT GGGCTGCACT 
ATTAAGCTCC GCGGAGCCTC AGAGTATGAG CTGGCACGGG TAAAAGAGAT CATCATTTTT 
ATGGTGTGTG TGGCATACCA CTCGCAGCTA GAGATCTCTT TCCTCATGGA TGAGTTCGCT 
ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT TTCCCATGTC TGCTGGAAAG CACCACTTTA 
CGGGAAGAGG ATGAAACTAG TGGGGAACGG GAAAGCCAAG ACAATGATGA GTCTACACTG 
GGAGATGACA ATCTCACACT TGTTCCAGGA GATGACTTTG AGACAGGATT TACAGGGACT 
ATCAAAGCCC ACAGTCGAGA GCCTTCCACC TCTGAATCTT CTCATAAAGA TGGAGAAAGC 
CCCAGAATAA ATAAAAATGC TTCAATTACC TCCTTCTCTG GAGGGGAGGA AGAAAATATA 
AAGACCTCCA CGCCACTTTC CTCCTTCTCT TCCACCTTTC CTCAGCCGGT GACACCACCT 
TTCCTGATAT CAGACGTAAA AGAAATGTCA CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG 
GAGAAGAACA AATATCTGGA GTCGACTCTG GTCCATCAGG ACAGCACAAG CTCTGAGACC 
TCCCTTCCCC CAACTCGGCT CTTTAGAGAC CCCTTACAGG ACGACACAGG CCTGTTTGTG 
ACAGAGCATG TAGCTTCTTC AGATGACCGC CTCAAATCCA TCTCTGCTTT ATTCAAACAG 
GAGCTAAAAG ACATCATCCT GTGCATTTCA CCCTTTATTA CCTTTCGAGA GCCTTTCTTG 
CTTACCCCAG CTGGACTGCG GTGTCCTAGC CGGGATTATT TCCCTGAACA GGTTTACCTT 
TCACCTCTGC TGAACAAGGA TTCAAAAGAA CTGGATGGAC GCCGCAAGAG GCAGCTGCTG 
AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG ACCAATGGTT CCGTTTCACA CCAGAGAGCC 
ATTCAGATCT TGCCCTGTCA CAAACTCACT AGTGCCCGAA TAGCAGAACA GGTAGGCAGC 
AGTCAGCAGC TGGCACGCAT GTTAGCGGAC TACCGTGCCC AAGGAGGACG TATCCGGCAA 
AGAGAGGGAA CACAATTTCG TGAAGCCCCT CCCCCAAAGG CGCCAATAAA GGCAGATAGT 
GAAGAAGATA AAGGTGCAGG AGTGATTGAC ATGACCTGGT CTACTAAGTT GGACTGTTTA 
AATCCAATCA ACCATCAGAG GCTCTGTGTT CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT 
AATGCCCCAA ACCCCTGCGT CAGTCCCTGG ATTGTTACAA TGGAGTTTTA TGGAAAGAAT 
GACTTGACTC TTGGTGTGTT TCTGGAGAGA TACTGTTTTA GACCATCTTA CCAGTGCCCC 
AGCATGTACT GTGAGACCCC CATGGTTCAC CACATCCGGC GCTTTGTGCA CGGTAAAGGC 
TGCGTCCAGA TTGTACTGAA AGAGCTGGAC TCGCCTGTTC CTGGATATCA GCACACAATC 
CTCAACTACT CATGGTGTCG CATCTGCAAA CAGGTGACTC CTGTGGTCCC TTTGTCTAAT 
GACTCTTGGT CCATGTCCTT CGCCAAGTAC CTTGAGCTTC GTTTCTATGG TCATCAGTAC 
ACCCGTCGGG CTAACGCTGA GCCTTGTGGC CACTCTATCC ATAAAGATTA TCACCAGTAC 
TTCTCTTACA ACCAGATGGT GGCATCCTTC AGCTACATTC CAGTGAGGCT GTTAGAGATC 
TGTCTGCCTC CCCCGAAGAT CATCATCAGG AACCAAGGTC CATCGAAATC CAGCTTGCAG 
CAGGATCTCA AAGACTTCTC CCAGAAGGTG GCTCAGGTGT ACCTGGCTAT AGATGACCGT 
CTTACCTCCT TAAAAACGGA CACATTCAGC AAGACCAGAG AGGAAAAAAT GGAGGACATG 
TTTGCACAGA AAGATATGGA GGAATCAGAG CTTCGGGGCT GGATAGAGAA GTTACAAGTG 
CGTCTGCAGA CGAGTGTGAT TGACTCTTCA CAACAACTAC AGGCTGTTCT GGAGTCAGTG 
GTGGTCAAAA AACAGGGCTT GTGTGAAACC CTGCAGTCCT GGAACAACAG ACTTCAAGAA 
CTGTTCCAGC AAGAGAAAGG CAGAAAACGT CTATCTGTTC CTCCTAGCCC AGGCAGACAC 
AGACAACCTA CAACAGATGA GAGCAAGACT AGTGCTTTGG AGTCTTCTCC TCGTAACCCT 
TCCCCAGTGG TCCCAAATGG AGACAAAGAG GACCGTCATC TCAATACATT TCCATCAAGT 
TCAGGGTCCT CGTCGTTACT ACAGTTACCA TCTCCAGCTG AACAACCTTC AGATGTCATT 
ACAAGCGGAC CATCTTTCCC TGATCAGGAC TCTGTTAGCA ATCCAGAGGA CATGTTTGAT 
GGACACTTGC TTGGTTCCAA TGATAGTCAG GTAAAGGAAA AGTCCACCAT GAAAACAATC 
CTGGCCAACC TGTTACCTGG AAACAGCTAC AATCCCATCC CTTTTCCTTT TGACCCAGAC 
AAGCACTATC TGATGTATGA GCATGAGAGA GTTCCTATAG CCGTGTGTGA GAGAGAGCCC 
AGCTCCATAA TTGCCTTTGC ACTCAGCTGC AAAGAGTATA AAACTGCCCT TGAAGAGCTC 
ACAAAGACAA CAGCAAAGAC TGGAGGTGAT GACACTTCTC AGACCATCAG TGCTGGAGAG 
AGCAGAGTGA AGAACAGTCC AGCCAAACCC AGTGACAACA GCATGTCACA GCTGAGCCGC 
AGCAGCGTCG ACGCTGATCC ACTCAAGGAG CCTGAAAGTG CAGACAAACA GAAGAAGCAG 
ACAGGGAACC CACATATTGA GCTGCAGTTC TCCGATGCAA ATGCTAAGTT CTACTGCCGG 
ATCTACTATG CTGAAGAGTT CCATAAAATG CGGGAGGAGA TCATGGAGAG CACAGAGGAT 
GATTTTGTGC GATCTCTTTC CCACTGTGTC AACTGGCAGG CGCGTGGTGG GAAATCTGGT 
GCAGTTTTCT ATGCCACTGA AGATGATCGG TTTATTCTGA AGCAGATGCC CAGATTAGAG 
GTCCAGTCCT TTTTAGACTT TGCACCCCAC TACTTTACGT ACATCACAGG AGCGGTTCAC 
CTAAAGCGAC CCACAGCACT TGCTAAGATT CTGGGAGTGT ACCGCATCGG CTACAAGAAC 
TCCCAGAACA ACACTGAGAA GAAGCTTGAC CTGCTTGTGA TGGAAAACCT TTTCTATGGA 
CGAAAGATGG CACAGGTGTT TGACCTGAAA GGATCGCTGA GAAACAGGAA CGTGAAGACG 
GACCAGGGAA AGGAGAGCTG TGAGGTGGTG CTCCTAGATG AGAACCTGCT CAAACTGGTG 
CATGACAATC CCCTTTATAT TCGCTCGCAC TGCAAGGCCA TCCTGCGTGC TGCCATCCAC 
AGCGACGCGC TCTTCCTGTC CAGCCACCTC ATCATTGATT ACTCCCTGCT GGTGGGCCGA 
GACGATTCCA CAGATGAGCT AGTTGTGGGG ATCATAGATT ATATCCGGAC TTTCACTTGG 
GATAAAAAGC TGGAGATGGT GGTCAAATCT ACTGGGATTC TTGGAGGTCA AGGTAAAATG 
CCCACAGTGG TGTCACCAGA GCTGTATCGA ACACGTTTCT GTGAGGCCAT GGATAAATAC 
TTCCTTATGG TCCCTGATCA CTGGAGTGGA CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167727.1
CDS	164..6391
Translation	MAADDKASSSSSVMDWSSEPPPSPTSPSHLTHFKPLTPEQDEPPLRSAYSSFVSLFRFNKEEGRPPSVTEKSQSASSSPQGPRRNWSSPSHSIHGSETHRKHPELLRRTSAASEGRRKSEAPLGSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSSSIGEDLSALSDSPCSVCVLEPTEPRTPVGGRKASRNIFLEEDLAWQSHQESQNSGLNSRLTVVQEDIGKSPARKRSASVTNLSLDRSGSSMVPAYESSVSPQNSRALPKTDHSEEERKILLDSFQLKDIWKKICHNTTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADENDYVTPSSSSPSKRTSVSSFHSAVDSDSAASISLNMEQDNVNFHIKKQAKYPHVPPYPAEQKMEVLLSEDGGQHISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQFVHIKKIPGGKKFDSAVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPNLVLVEKTVSRIAQDMLLEHGIAIVINVKPQVLDRVSRMTQGDLVISMDQLLTKPRLGTCHKFYLHSFQLPNSEVKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIIIFMVCVAYHSQLEISFLMDEFAMPPSLAESSSFPCLLESTTLREEDETSGERESQDNDESTLGDDNLTLVPGDDFETGFTGTIKAHSREPSTSESSHKDGESPRINKNASITSFSGGEEENIKTSTPLSSFSSTFPQPVTPPFLISDVKEMSQEVVKGSGEGEKNKYLESTLVHQDSTSSETSLPPTRLFRDPLQDDTGLFVTEHVASSDDRLKSISALFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDSKELDGRRKRQLLKESSPGSGSLTNGSVSHQRAIQILPCHKLTSARIAEQVGSSQQLARMLADYRAQGGRIRQREGTQFREAPPPKAPIKADSEEDKGAGVIDMTWSTKLDCLNPINHQRLCVLFSSSSAQSNNAPNPCVSPWIVTMEFYGKNDLTLGVFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGKGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLLEICLPPPKIIIRNQGPSKSSLQQDLKDFSQKVAQVYLAIDDRLTSLKTDTFSKTREEKMEDMFAQKDMEESELRGWIEKLQVRLQTSVIDSSQQLQAVLESVVVKKQGLCETLQSWNNRLQELFQQEKGRKRLSVPPSPGRHRQPTTDESKTSALESSPRNPSPVVPNGDKEDRHLNTFPSSSGSSSLLQLPSPAEQPSDVITSGPSFPDQDSVSNPEDMFDGHLLGSNDSQVKEKSTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKTALEELTKTTAKTGGDDTSQTISAGESRVKNSPAKPSDNSMSQLSRSSVDADPLKEPESADKQKKQTGNPHIELQFSDANAKFYCRIYYAEEFHKMREEIMESTEDDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVHLKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDALFLSSHLIIDYSLLVGRDDSTDELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWSGLGLNC

Target ORF information:

RefSeq Version	XM_005167670.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167670.4

ORF Insert Sequence:

ATGGCTGCTG ATGACAAGGC TTCCTCCTCG TCCTCTGTGA TGGACTGGAG TTCGGAGCCG 
CCTCCTTCGC CCACCAGTCC CTCCCATCTG ACACACTTCA AACCCCTGAC CCCCGAGCAG 
GATGAGCCAC CGCTGCGCTC CGCCTACAGC TCCTTCGTCA GCCTGTTCCG CTTCAACAAA 
GAGGAAGGAC GGCCTCCTTC AGTGACGGAA AAAAGTCAAT CGGCCTCATC ATCACCACAG 
GGGCCGCGAC GCAACTGGTC TAGTCCCTCT CATTCAATAC ATGGCTCTGA AACACACAGG 
AAACATCCAG AACTACTCAG AAGAACATCC GCTGCTTCAG AGGGTAGACG GAAATCAGAA 
GCTCCTCTGG GCAGCCACGA TCCCAGAACA GCTGTCCAGC TTCGCACAGC TCTCAAGAGG 
CTCAAAGAGA TCATGGAAGG GAAAAGTCAG GACAGTGATC TGAAACAGTA CTGGATGCCA 
GATAGCCAGT GTAAGGAGTG CTATGACTGC AATGAGAAAT TCACAACCTT CCGACGCCGT 
CACCATTGTC GACTATGTGG ACAAATATTC TGCAGCCGAT GCTGCAACCA GGAAATTCCT 
GGCAAGTTCA TGGGCTACAC GGGCGATTTA AGGGCTTGTA CATACTGTCG TAAGATAGCA 
TTAAGCTATG CTCACTCAAC AGACTCAAGC TCCATTGGAG AAGATCTAAG TGCTTTGTCG 
GACTCGCCTT GCTCCGTCTG TGTTCTGGAG CCCACTGAAC CACGCACACC TGTAGGAGGT 
CGCAAAGCCA GCCGCAATAT CTTCCTGGAA GAGGACCTAG CCTGGCAAAG TCATCAGGAA 
TCTCAGAACA GCGGTCTCAA TTCCAGACTG ACAGTGGTCC AAGAGGATAT TGGAAAGTCA 
CCTGCCAGGA AGAGGTCAGC TAGTGTGACC AATTTGTCTT TGGACCGTTC CGGCTCATCC 
ATGGTTCCTG CCTATGAGAG TTCGGTTAGT CCACAGAACA GCCGAGCTCT ACCCAAGACC 
GACCACAGCG AAGAAGAGCG AAAGATCCTT CTAGATTCTT TTCAACTTAA AGATATATGG 
AAGAAGATTT GCCACAACAC TACTGGGATG GAGTTCCAGG ACCATCGGTA CTGGCTGCGC 
ACTTACCCCA ACTGCATTGT GGGTAAAGAG TTGGTCAACT GGCTTTTAAG AAATGGCACT 
ATTTCCACTA GGGCTCAAGC CATAGCTATA GGACAGGCTT TGGTAGATGG CCGCTGGCTT 
GACTGTGTAA CCCATCATGA TCAGATCTTC CGTGATGAGT ACGCCCTTTA TCGCCCCCTT 
CAGAGCACAG AGTTCTCCGA AACTCCTTCT CCAGACAGTG ACAGTGTTAA TTCCCTGGAG 
GGACACTCTG AACCGTCATG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACCGAGCAG 
CTAGCAGATG AAAATGACTA TGTCACACCA AGCTCGTCCA GCCCCAGCAA AAGAACATCT 
GTCAGCAGTT TCCACTCTGC AGTAGACAGT GACTCGGCCG CCTCCATCAG TCTAAACATG 
GAGCAGGACA ACGTCAATTT CCACATTAAG AAGCAGGCCA AGTACCCCCA TGTGCCTCCC 
TACCCAGCTG AGCAAAAAAT GGAAGTCTTG CTCTCCGAAG ATGGAGGTCA GCATATATCC 
ATCAGTGATG CATTCATCAA AGAGTCTTTG TTCAACCGGA GGGTTGAGGA GAAAGCTAAA 
GAGATGCTCT TCACTCCTCT CGGCTGGCAT CACAGCTCTC TGGATCAGCT GCGGGAAGAG 
AATGGGGAAA AGAAAGCGAT GGAGCGGTTG CTGTCAGCAA ATCACAGCCA CATGATGGCG 
CTGCTGCAGC AACTGCTGTA CAGTGAATCA CTGTCTCTGT CGTGGCGTGA CATCATCGTA 
CCTGTGGTGA GGCAGGTGGT GCAGACGGTG CGACCGGATG TGCGCAGCTG TGACGATGAT 
ATGGACATCC GTCAGTTTGT CCACATCAAG AAGATTCCAG GAGGAAAGAA GTTTGACTCT 
GCTGTAGTGA ATGGTTTTGT TTGCACAAAG AATATCGCAC ACAAAAAGAT GAACTCTTAC 
ATCAAAAACC CCAAGATCCT TCTTCTCAAA TGCTCCATTG AGTATCTATA TAGAGAGGAG 
ACCAAGTTCA CCTGCATTGA CCCAATTGTT TTACAGGAGC GTGAGTTTCT TAAGAACTAT 
GTTCAGAGGA TTGTTGATGT CCGACCAAAC TTAGTCCTGG TGGAGAAGAC TGTATCGCGA 
ATTGCTCAGG ACATGCTACT AGAGCACGGC ATTGCCATTG TGATTAATGT CAAACCTCAA 
GTACTGGACC GTGTGAGTCG AATGACTCAG GGAGATTTAG TCATTTCAAT GGATCAGCTT 
CTTACAAAAC CTCGTCTGGG TACCTGCCAC AAGTTTTACT TGCACTCCTT CCAGCTACCA 
AATAGTGAAG TGAAAACCCT GATGTTTTTT GAGGGCTGTC CTCCTCAGCT GGGCTGCACT 
ATTAAGCTCC GCGGAGCCTC AGAGTATGAG CTGGCACGGG TAAAAGAGAT CATCATTTTT 
ATGGTGTGTG TGGCATACCA CTCGCAGCTA GAGATCTCTT TCCTCATGGA TGAGTTCGCT 
ATGCCTCCCA GTCTGGCTGA GAGTTCTTCT TTCCCATGTC TGCTGGAAAG CACCACTTTA 
CGGGAAGAGG ATGAAACTAG TGGGGAACGG GAAAGCCAAG ACAATGATGA GTCTACACTG 
GGAGATGACA ATCTCACACT TGTTCCAGGA GATGACTTTG AGACAGGATT TACAGGGACT 
ATCAAAGCCC ACAGTCGAGA GCCTTCCACC TCTGAATCTT CTCATAAAGA TGGAGAAAGC 
CCCAGAATAA ATAAAAATGC TTCAATTACC TCCTTCTCTG GAGGGGAGGA AGAAAATATA 
AAGACCTCCA CGCCACTTTC CTCCTTCTCT TCCACCTTTC CTCAGCCGGT GACACCACCT 
TTCCTGATAT CAGACGTAAA AGAAATGTCA CAGGAAGTGG TCAAGGGGTC AGGTGAAGGG 
GAGAAGAACA AATATCTGGA GTCGACTCTG GTCCATCAGG ACAGCACAAG CTCTGAGACC 
TCCCTTCCCC CAACTCGGCT CTTTAGAGAC CCCTTACAGG ACGACACAGG CCTGTTTGTG 
ACAGAGCATG TAGCTTCTTC AGATGACCGC CTCAAATCCA TCTCTGCTTT ATTCAAACAG 
GAGCTAAAAG ACATCATCCT GTGCATTTCA CCCTTTATTA CCTTTCGAGA GCCTTTCTTG 
CTTACCCCAG CTGGACTGCG GTGTCCTAGC CGGGATTATT TCCCTGAACA GGTTTACCTT 
TCACCTCTGC TGAACAAGGA TTCAAAAGAA CTGGATGGAC GCCGCAAGAG GCAGCTGCTG 
AAAGAGTCCA GCCCAGGTTC TGGTAGCCTG ACCAATGGTT CCGTTTCACA CCAGAGAGCC 
ATTCAGATCT TGCCCTGTCA CAAACTCACT AGTGCCCGAA TAGCAGAACA GGTAGGCAGC 
AGTCAGCAGC TGGCACGCAT GTTAGCGGAC TACCGTGCCC AAGGAGGACG TATCCGGCAA 
AGAGAGGGAA CACAATTTCG TGAAGCCCCT CCCCCAAAGG CGCCAATAAA GGCAGATAGT 
GAAGAAGATA AAGGTGCAGG AGTGATTGAC ATGACCTGGT CTACTAAGTT GGACTGTTTA 
AATCCAATCA ACCATCAGAG GCTCTGTGTT CTGTTCAGTA GCTCTTCAGC ACAGTCTAAT 
AATGCCCCAA ACCCCTGCGT CAGTCCCTGG ATTGTTACAA TGGAGTTTTA TGGAAAGAAT 
GACTTGACTC TTGGTGTGTT TCTGGAGAGA TACTGTTTTA GACCATCTTA CCAGTGCCCC 
AGCATGTACT GTGAGACCCC CATGGTTCAC CACATCCGGC GCTTTGTGCA CGGTAAAGGC 
TGCGTCCAGA TTGTACTGAA AGAGCTGGAC TCGCCTGTTC CTGGATATCA GCACACAATC 
CTCAACTACT CATGGTGTCG CATCTGCAAA CAGGTGACTC CTGTGGTCCC TTTGTCTAAT 
GACTCTTGGT CCATGTCCTT CGCCAAGTAC CTTGAGCTTC GTTTCTATGG TCATCAGTAC 
ACCCGTCGGG CTAACGCTGA GCCTTGTGGC CACTCTATCC ATAAAGATTA TCACCAGTAC 
TTCTCTTACA ACCAGATGGT GGCATCCTTC AGCTACATTC CAGTGAGGCT GTTAGAGATC 
TGTCTGCCTC CCCCGAAGAT CATCATCAGG AACCAAGGTC CATCGAAATC CAGCTTGCAG 
CAGGATCTCA AAGACTTCTC CCAGAAGGTG GCTCAGGTGT ACCTGGCTAT AGATGACCGT 
CTTACCTCCT TAAAAACGGA CACATTCAGC AAGACCAGAG AGGAAAAAAT GGAGGACATG 
TTTGCACAGA AAGATATGGA GGAATCAGAG CTTCGGGGCT GGATAGAGAA GTTACAAGTG 
CGTCTGCAGA CGAGTGTGAT TGACTCTTCA CAACAACTAC AGGCTGTTCT GGAGTCAGTG 
GTGGTCAAAA AACAGGGCTT GTGTGAAACC CTGCAGTCCT GGAACAACAG ACTTCAAGAA 
CTGTTCCAGC AAGAGAAAGG CAGAAAACGT CTATCTGTTC CTCCTAGCCC AGGCAGACAC 
AGACAACCTA CAACAGATGA GAGCAAGACT AGTGCTTTGG AGTCTTCTCC TCGTAACCCT 
TCCCCAGTGG TCCCAAATGG AGACAAAGAG GACCGTCATC TCAATACATT TCCATCAAGT 
TCAGGGTCCT CGTCGTTACT ACAGTTACCA TCTCCAGCTG AACAACCTTC AGATGTCATT 
ACAAGCGGAC CATCTTTCCC TGATCAGGAC TCTGTTAGCA ATCCAGAGGA CATGTTTGAT 
GGACACTTGC TTGGTTCCAA TGATAGTCAG GTAAAGGAAA AGTCCACCAT GAAAACAATC 
CTGGCCAACC TGTTACCTGG AAACAGCTAC AATCCCATCC CTTTTCCTTT TGACCCAGAC 
AAGCACTATC TGATGTATGA GCATGAGAGA GTTCCTATAG CCGTGTGTGA GAGAGAGCCC 
AGCTCCATAA TTGCCTTTGC ACTCAGCTGC AAAGAGTATA AAACTGCCCT TGAAGAGCTC 
ACAAAGACAA CAGCAAAGAC TGGAGGTGAT GACACTTCTC AGACCATCAG TGCTGGAGAG 
AGCAGAGTGA AGAACAGTCC AGCCAAACCC AGTGACAACA GCATGTCACA GCTGAGCCGC 
AGCAGCGTCG ACGCTGATCC ACTCAAGGAG CCTGAAAGTG CAGACAAACA GAAGAAGCAG 
ACAGGGAACC CACATATTGA GCTGCAGTTC TCCGATGCAA ATGCTAAGTT CTACTGCCGG 
ATCTACTATG CTGAAGAGTT CCATAAAATG CGGGAGGAGA TCATGGAGAG CACAGAGGAT 
GATTTTGTGC GATCTCTTTC CCACTGTGTC AACTGGCAGG CGCGTGGTGG GAAATCTGGT 
GCAGTTTTCT ATGCCACTGA AGATGATCGG TTTATTCTGA AGCAGATGCC CAGATTAGAG 
GTCCAGTCCT TTTTAGACTT TGCACCCCAC TACTTTACGT ACATCACAGG AGCGGTTCAC 
CTAAAGCGAC CCACAGCACT TGCTAAGATT CTGGGAGTGT ACCGCATCGG CTACAAGAAC 
TCCCAGAACA ACACTGAGAA GAAGCTTGAC CTGCTTGTGA TGGAAAACCT TTTCTATGGA 
CGAAAGATGG CACAGGTGTT TGACCTGAAA GGATCGCTGA GAAACAGGAA CGTGAAGACG 
GACCAGGGAA AGGAGAGCTG TGAGGTGGTG CTCCTAGATG AGAACCTGCT CAAACTGGTG 
CATGACAATC CCCTTTATAT TCGCTCGCAC TGCAAGGCCA TCCTGCGTGC TGCCATCCAC 
AGCGACGCGC TCTTCCTGTC CAGCCACCTC ATCATTGATT ACTCCCTGCT GGTGGGCCGA 
GACGATTCCA CAGATGAGCT AGTTGTGGGG ATCATAGATT ATATCCGGAC TTTCACTTGG 
GATAAAAAGC TGGAGATGGT GGTCAAATCT ACTGGGATTC TTGGAGGTCA AGGTAAAATG 
CCCACAGTGG TGTCACCAGA GCTGTATCGA ACACGTTTCT GTGAGGCCAT GGATAAATAC 
TTCCTTATGG TCCCTGATCA CTGGAGTGGA CTTGGGCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	pikfyve
Entrez Gene ID	337690
Full Name	phosphoinositide kinase, FYVE finger containing
Synonyms	pip5k3,wu:fo93c03
General protein information	Preferred Names phosphoinositide kinase, FYVE finger containing Names 1-phosphatidylinositol 3-phosphate 5-kinase phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Mus musculus (house mouse)	Pikfyve	NP_035216.2
Canis lupus familiaris (dog)	PIKFYVE	XP_005640650.1
Rattus norvegicus (Norway rat)	Pikfyve	XP_006245139.1
Gallus gallus (chicken)	PIKFYVE	XP_004942735.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005216	XP_314118.4
Drosophila melanogaster (fruit fly)	fab1	NP_995880.1
Homo sapiens (human)	PIKFYVE	NP_055855.2
Bos taurus (cattle)	PIKFYVE	NP_001192396.1
Pan troglodytes (chimpanzee)	PIKFYVE	XP_516057.2
	PIKFYVE	XP_001108540.1
Danio rerio (zebrafish)	pikfyve	NP_001120777.1

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pikfyve cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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