cnot1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cnot1
Entrez Gene ID	448949
Full Name	CCR4-NOT transcription complex, subunit 1
Synonyms	cdc39-like,im:7141761,kiaa1007l,wu:fc58h04,zgc:152902
General protein information	Preferred Names CCR4-NOT transcription complex, subunit 1 Names CCR4-NOT transcription complex subunit 1 CCR4-associated factor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001079951.2	NP_001073420.1	CCR4-NOT transcription complex subunit 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CNOT1	NP_057368.3
Mus musculus (house mouse)	Cnot1	NP_835179.1
Danio rerio (zebrafish)	cnot1	NP_001073420.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009057	XP_319808.4
Drosophila melanogaster (fruit fly)	Not1	NP_724798.3
Caenorhabditis elegans (roundworm)	let-711	NP_498516.2
Oryza sativa (rice)	Os10g0556801	NP_001176268.1
Pan troglodytes (chimpanzee)	CNOT1	XP_511007.2
Bos taurus (cattle)	CNOT1	NP_001192968.1
Gallus gallus (chicken)	CNOT1	NP_001264062.1
Rattus norvegicus (Norway rat)	Cnot1	NP_001128312.1
	CNOT1	XP_001102008.2
Canis lupus familiaris (dog)	CNOT1	XP_535279.4
Arabidopsis thaliana (thale cress)	AT1G02080	NP_171710.4
Xenopus tropicalis (tropical clawed frog)	cnot1	NP_001090658.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Large-scale discovery of novel genetic causes of developmental disorders.

Nature519(7542)223-8(2015 Mar)

Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA).
Sander JD, Dahlborg EJ, Goodwin MJ, Cade L, Zhang F, Cifuentes D, Curtin SJ, Blackburn JS, Thibodeau-Beganny S, Qi Y, Pierick CJ, Hoffman E, Maeder ML, Khayter C, Reyon D, Dobbs D, Langenau DM, Stupar RM, Giraldez AJ, Voytas DF, Peterson RT, Yeh JR, Joung JK
Nature methods8(1)67-9(2011 Jan)

Identification of 315 genes essential for early zebrafish development.
Amsterdam A, Nissen RM, Sun Z, Swindell EC, Farrington S, Hopkins N
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cnot1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cnot1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00117	NM_001079951.2 Latest version!	Danio rerio CCR4-NOT transcription complex, subunit 1 (cnot1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cnot1 ( NM_001079951.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073420.1 Danio rerio CCR4-NOT transcription complex, subunit 1 (cnot1), mRNA.

Price & Availability↑

CloneID	ODa00117
Clone ID Related Accession (Same CDS sequence)	NM_001079951.2
Accession Version	NM_001079951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	CCR4-NOT transcription complex subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC128655.1. On Aug 31, 2012 this sequence version replaced NM_001079951.1. ##Evidence-Data-START## Transcript exon combination :: BC128655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCTTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACTAAGAAAA ACTACAGAGC CAGCCAGCAG GAAATACAAC ATATTGTGAA TCGTCACGGC 
CCTGAGGCAG ACCGGCATCT ATTACGCTGT CTCTTTTCCC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCACCAG ACACAGTTTC TGATCCAGGA GTGCGTGTCT 
CTTATTACGA AACCAAATTT TATTTCAACG CTTTGCTACG CCATTGACAA TCCCCTGCAC 
TATCAAAAGA GTTTGAAACC GTCACCTCAT TTATTTACTC AGCTGAGTAA AGTTCTCAAG 
CTAAGCAAGG TTCAGGAGGT GATTTTGGGC CTTGCTCTGA GCAACTCCAG CAACGCAGAC 
CTTCGAGGGT TTGCTGCTCA GTTTGTGAAG CAGAAGCTCC CTGACCTCCT CCGCTCGTAC 
GTGGACGCCG ACCTCGGAGG GAATCAGGAA GGTGGCTTCC AGGACATTGC CATAGAGGTT 
CTGCATCTGC TCCTCTCCCA TCTTCTGTTC GGTCAAAAGG GCTCGAGCGG AGTCGGACAA 
GAGCAGATTG ACGCGTTCCT CAAAACACTG TGCAGAGATT TCCCGCAGGA GCGCTGTCCT 
GTGGTGCTTG CACCACTGCT GTATCCTGAC AAACGGGACA TTCTCATGGA CAGGATCCTG 
CCAGACTCTG GAGATTTAAA TAAGACCATG ATGGAGAGTT CTCTTGCTGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTCTT GAAGAGTGCA GGAACATAAT CCTGCAGTAT 
GGGGTGCGAG AGGTGACAGC CAGTCAGGTG GCCAGAGTGC TGGGCATGAT GGCCCGCACT 
CACTCAGGGC TGTCTGACGG CATCAGCCTT CAGACCATCA CCAATCCAGT GGGCGGTGGT 
GGGATCTGGA GTGACGGGAA GGACAAGAGT GACAGCTCTC AGGCCTGGAA TGTTGAAGTT 
CTAATTGATG TGGTCAAAGA AGTGAACCCT AATCTGAATT TCAAAGAGGT GACCTATGAG 
CTGGATCACC CAGGCTTCCT GATCCGGGAC AGTAAGGGGC TTCAGATAGT TGTTTATGGG 
ATCCAGAGGG GTCTGGGAAT GGAAGTGTTC CCGGTGGATC TCATTTACAG ACCATGGAAG 
CATGCAGAAG GACAGTTGTC GTTCATCCAG CACTCTCTAT TGAGTCCTGA AGTGTTCTGC 
TTTGCTGACA ACCCTTGCCA TACTGTTGCT ATTGACACCT TGAAGGCTCC ACCTGAGGAT 
GACAACAGGG AGATAGCCAC ATGGAAGAGC TTGGACCTTG TAGAGAGCCT CTTGCGCCTC 
TCTGAAGTTG GGCATTATGA ACAGGTGAAG CAGCTTTTTA GCTTCCCCAT CAAACACTGC 
CCGGACATGC TGGTGTTGGC GCTCCTGCAG ATCAGTACCT CCTGGCACAC CCTACGTCAT 
GAGCTCATCT CCACCCTCAT GCCCATCTTC CTGGGCAACC ACCCTAACTC TGCTATCATC 
TTGCACTATG CCTGGCATGG ACAGGGCCAG TCCCCATCCA TTCGCCAGCT GATCATGCAC 
TCTATGGCCG AGTGGTACAT GAGAGGGGAG CAATATGACC AGGCCAAGCT ATCTCGCATC 
CTGGATGTGG CTCAGGACTT GAAGTCTCTA TCGATGCTGC TGAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTAGCTGC ACTTGCCTCT CGCCGTGAAT ACCTCAAACT TGACAAATGG 
CTCACTGACA AAATACGGGA GCATGGGGAG CCATTCATCC AAGCGTGTGT CACATTTCTG 
AAGAGACGCT GCCCCTCTAT TATGGGTGGT TTGGCCCCTG AGAAAGACCA GCCGAAAAGT 
GCTCAACTTC CTCCTGAAAC ACTGGCTACC ATGCTTGCAT GTTTGCAGTC TTGTGCTGGG 
AGTGTGTCTC AAGAGCTGTC AGAGACAATC TTGACCATGG TGGCCAACTG TAGCAATGTG 
ATGAACAAGG CTCGGCAGCC GCCACCAGGA GTCCTGCCAA AGGGACGAGC GCCCAGCACC 
AGCAGTTTAG ATGCTATCTC ACCTGTGCAG ATGGACCCTC TCTCTGCAAT GGGCTCTTTA 
AGTCTAGGTG TCTCGTCCAC CTCTCACACC CCGAGCATGC AAGGATTCCC CAGCCTGCAG 
GGCTCTGCCT TCAGTAACCC CCAGTCCCCA GCCAAAGCCT TCTCAAACCT GCCCAACCCA 
AACCCCAGCA CAGCTTTCCC AGGAATCAAC CCCCTCTCTT CACAGCTCCA AGGTCCTCTG 
AGCACAAGCT TGTCTGGGAT TGGCTCAGGT TTGGGCATGC CCACCGTCAG CAGTGATGTC 
TTCAGCGCCA GGAAGATGAG CACACCGGGG CTAAACCCCC CAACCTTCCA GCAGACTGAC 
CTCTCTCAGG TGTGGCCCGA GGCAAACCAG CACTTTAGTA AGGAGATTGA CGATGAAGCT 
AACAGCTACT TCCAGCGCAT CTACAACCAT CCGCCACATC CCACAATGTC TGTGGACGAG 
GTGCTGGAGA TGCTGCAAAG ATTTAAGGAC TCAAACATCA AGCGGGAACG AGAAGTTTTT 
AACTGCATGC TGAGGAATCT GTTTGAAGAG TACCGATTCT TTCCTCAGTA TCCAGACAAA 
GAGCTGCACA TCACCGCCTG CCTCTTTGGG GGAATCATTG AGAAGGGCCT GGTCACATAC 
ATGGCGCTGG GCTTGGCCTT AAGATACGTG CTGGAAGCCT TACGCAAACC TTTCGGATCA 
AAGATGTATT ACTTTGGGAT CGCTGCACTA GATAGATTTA AAAATAGACT GAAGGACTAT 
CCCCAGTACT GTCAGCACTT GGCATCAATA GCCCATTTCC TACAGTTTCC ACACCATTTA 
CAAGAGTATA TAGAGTATGG CCAACAGTCA AGAGACCCTC CTGTGAAGAT GCAAGGCTCC 
ATTACCACCC CAGGCAGCCT GGCTCTAGCC CAAGCGCAAG CTCAGTCTCA GCCCCCTAAA 
GCCCCCCAAC CTGGCCAAGC CAGCACCTTA GTGACAACAG CCACCACGAC CACCACCGCT 
GCCAAAACCA CAACCATCAC AAGACCTACA GCTGTTGGAC CCAAGAAGGA TGTGCCACCT 
TCAATCAACA CCACAAACAT TGACACCTTG TTAGTGGCCA CAGATCAGAC CGAAAGGATC 
GTGGAGCCCC CAGAAAATGT TCAGGAGAAA ATTGCTTTTA TTTTCAATAA CTTGTCCCAG 
TCAAACATGT CCCAAAAGGT GGAGGAGCTA AAAGAGACTG TGAAAGAAGA ATTCATGCCC 
TGGGTTTCTC AGTACCTGGT AATGAAGCGT GTCAGCATTG AGCCCAATTT CCACAGCCTG 
TATTCTAACT TCTTGGACAC ACTGAAAAAC CCAGAGTTTG TCAAGATGGT TCTCAATGAG 
ACTTACAGAA ACATCAAGGT CCTCCTTACC TCGGATAAGG CAGCTGCTAA TTTCTCAGAT 
CGATCCCTGC TGAAGAATTT GGGTCACTGG CTTGGAATGA TCACCCTAGC AAAAAACAAA 
CCCATCCTGT ATACAGACCT GGAGCTGAAA TCTCTTTTGT TGGAGGCTTA TGTGAAAGGC 
CAGCAGGAGT TACTGTATGT TGTCCCTTTT GTGGCCAAAG TCTTGGAATC AAGTCTGCGG 
AGTGTGATCT TCAGACCGCA GAACCCTTGG ACAATGGGCA TCATGAATGT ACTGGCTGAA 
CTCCACCAGG AGCATGACTT AAAGCTTAAC CTCAAATTTG AGATTGAGGT ACTCTGCAAG 
AATCTGTCTA TGGACATCAC TGATCTTAAG CCAGGGAACC TGCTGAGAGA CAAAGATAAG 
CTGAAGACCT TGGAGGAGCA GCTCTCCGCA CCAAAGAAAG AGACCAAGCC TCCTGAAGAA 
TTGCTGCCTA TAGTTACAAC AGACTCTGTT CCTTTCACAG CTGCTCCCTC AACTCCGGCC 
ACCACCACGG CCTGTACAGC CACAGGGCCA CCTACACCTC AGTTCAGCTA TCATGACATC 
AATGTGTATG CATTGGCTGG ACTGGCCCCA CACATAAACA TCAATGTTAA CATCCCACTG 
CTACAGGCCC ACCCTCAGCT GAAGCAGTGC GTGAGACCAG CAATCGAACG TGCCGTACAG 
GAGCTTGTGC ACCCAGTGGT GGACCGATCC ATCAAGATCG CCATGACAAC CTGTGAGCAG 
ATAGTCAGGA AGGACTTTGC TCTAGACTCA GAGGAGTCTC ATATGCGTGT GGCTGCTCAC 
CACATGATGC GTAACCTGAC TGCCGGCATG GCCATGATCA CCTGCAGAGA GCCCCTGCTG 
ATGAGCATTG CCACCAATCT GAAGAACAGC TTTGCTGCAG CTCTTAGGGC TCCCACACCC 
CAGCAGAGAG AGATGATGGA GGAGGCGGCT GCTCGCATCG CACAGGACAA CTGTGAGCTT 
GCATGTTGCT TTATCCAGAA AACAGCAGTG GAGAAAGCTG GCCCGGAGAT GGATAAGAGA 
CTGGCAACTG AATTTGAGCT GAGGAAGCAT GCTCGCCAAG AAGGAAGACG CTACTGTGAC 
CCCATGGTGC TCACCTATCA GGCAGAGCGC ATGCCTGAAC AGATCAGACT GAAGGTTGGT 
GGAGTGGATC CTAAACAGCT GGCTGTTTAT GAGGAGTTCG CCCGCAACGT TCCAGGCTTC 
CTACCAAGTA ATGACCTGTC TCAACCAACT GGTTTTCTTG CTCAACCAAT GAAGCAACAA 
GCATGGCCCA CTGATGATGT GGCTCACATC TATGAGAAGT GCATTTCTGA CCTGGAACAG 
CACCTGCATG CAATTCCACC TGCACTGGCC ATGAACCCAC AGACCCAGGC CATACGTAGC 
CTGCTGGAGG CAGTTGTCAT GGCAAGGAAC TCCCGTGATG GCATTGCTGC CCTGGGACTT 
TTGCAGAAGG CTGTGGAGGG TCTTCTTGAT GCCACCAGTG GTGCTGATCC TGAATTGTTA 
CTAAGCTACA GGGAGTGCCA TCTGTTGGTG CTGAAAGCCT TACAGGATGG CCGTGCCTAC 
GGTCCACAGT GGTGCAACAA GCAAATCACC AGATGCCTGA TTGAGTGTAG AGATGAATAC 
AAGTACAATG TTGAGGCTGT GGAGCTTCTC ATCAGAAACC ACCTGGTCAA CATGCAGCAG 
TATGATTTGC ACCTAGCTCA GTCCATGGAG AATGGCCTCA ACTACATGGC AGTGGCATTT 
GCCATGCAGC TGGTGAAACT CTTGCTGGTT GATGAGCGCA GCGTGAGCCA CATCACTGAA 
GCAGATCTTT TCCACACTAT CGAGACACTG ATGAGGACCA GCGCCCACTC CAGAGCCAAC 
GCTCCTGAAG GTTTGCCACA GCTTATGGAT GTTGTCCGCT CCAACTATGA GGCCATGATT 
GATCGTCACC ATGGTGGACC AAATTTCATG ATGCACTCTG GAATTTCTCA AGCATCGGAG 
TACGATGACC CACCAGGCCT TAGAGAAAAG GCAGAGTACC TGCTGAGAGA ATGGGTCAAC 
CTGTACCACT CAGCAGCAGC AGGAAGGGAC AGCACCAAGG CCTTCTCTGC ATTTGTTGGC 
CAGATGCACC AGCAGGGTAT CCTAAAGACT GATGACTTGA TCACTCGCTT TTTCCGGCTA 
TGCACAGAAA TGTGTGTAGA GATCAGTTAT CGCGCTCAAG CTGAACAGCA GCACCCAACC 
ACCAGTCCTG CCATTATTAG GGCCAAATGC TACCATAACT TGGATGCTTT CGTTCGGCTT 
ATTGCCCTTC TGGTCAAACA CTCTGGAGAG GCCACCAACA CAGTCACTAA AATTAACCTG 
CTCAACAAGG TTTTGGGCAT TGTGGTGGGT GTGTTAATCC AGGATCATGA TGTGAGGCAG 
ACAGAGTTCC AGCAGCTTCC ATACCACAGA ATCTTCATCA TGCTCTTACT GGAGCTAAAC 
GCACCAGAGC ATGTGCTGGA GACCATCAAC TTCCAGACTC TCACTGCCTT CTGCAACACC 
TTCCACATCC TGAGGCCGAC TAAAGCACCT GGTTTTGTGT ATGCTTGGCT AGAGCTCATC 
TCTCACCGCA TCTTCATTGC CAGGATGCTT GCTCACACCC CACAACAGAA GGGTTGGCCT 
ATGTATGCCC AGCTGCTGAT AGACTTGTTT AAATATCTTG CACCCTTCCT AAGGAATGTT 
GAACTCAACA AACCTATGCA AATTCTCTAC AAGGGAACAC TTCGTGTGCT GCTTGTCCTG 
CTACATGACT TCCCAGAATT CCTGTGTGAC TACCATTACG GATTCTGTGA TGTCATCCCA 
CCCAACTGCA TCCAGCTGAG GAATCTGATC CTGAGTGCCT TCCCCCGCAA CATGAGGCTT 
CCGGACCCCT TCACCCCTAA TTTAAAGGTG GATATGCTGA GTGAGATCAA CATCGCTCCC 
CGCATCCTCA CAAACTTCAC TGGTGTGATG CCGTCCCAGT TTAAGAAGGA TCTTGACTCC 
TACCTCAAGA CCCGCTCTCC TGTCACCTTC CTCTCTGAGC TGCGCAGCAA CCTGCAGGTG 
TCCAATGAGC CAGGCAACCG CTACAACATT CAGCTGATCA ACGCTCTGGT GCCGTACGTT 
GGAACCCAGG CCATTGCCCA CATTCACAAC AAGGGCAGCA CTCCCTCCAT GAGCACCATC 
ACACACTCGG CACACATGGA CATCTTCCAG AACCTCGCAG TCGATCTAGA CACTGAGGGT 
CGCTATCTGT TCCTGAATGC GATAGCCAAT CAGCTGCGTT ATCCAAACAG CCACACGCAC 
TACTTCAGCT GTACCATGCT GTATCTGTTT GCCGAGGCCA ATGCTGAAGC CATTCAAGAG 
CAGATCACCA GGGTCTTGCT GGAGAGGCTG ATTGTGAACA GGCCACACCC GTGGGGTCTG 
CTCATCACCT TCATAGAGCT GATCAAGAAC CCTGCCTTCA AGTTCTGGAG CCATGATTTT 
GTGCACTGCG CCCCAGAGAT CGAGAAGTTG TTCCAGTCAG TGGCTCAGTG CTGTATGGGG 
CAGAAGCAGG CCCAGCAGGT GATGGAGGGA ACCGGTGCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073420.1
CDS	308..7432
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECVSLITKPNFISTLCYAIDNPLHYQKSLKPSPHLFTQLSKVLKLSKVQEVILGLALSNSSNADLRGFAAQFVKQKLPDLLRSYVDADLGGNQEGGFQDIAIEVLHLLLSHLLFGQKGSSGVGQEQIDAFLKTLCRDFPQERCPVVLAPLLYPDKRDILMDRILPDSGDLNKTMMESSLADFMQEVGYGFCASLEECRNIILQYGVREVTASQVARVLGMMARTHSGLSDGISLQTITNPVGGGGIWSDGKDKSDSSQAWNVEVLIDVVKEVNPNLNFKEVTYELDHPGFLIRDSKGLQIVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLLSPEVFCFADNPCHTVAIDTLKAPPEDDNREIATWKSLDLVESLLRLSEVGHYEQVKQLFSFPIKHCPDMLVLALLQISTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHSMAEWYMRGEQYDQAKLSRILDVAQDLKSLSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACVTFLKRRCPSIMGGLAPEKDQPKSAQLPPETLATMLACLQSCAGSVSQELSETILTMVANCSNVMNKARQPPPGVLPKGRAPSTSSLDAISPVQMDPLSAMGSLSLGVSSTSHTPSMQGFPSLQGSAFSNPQSPAKAFSNLPNPNPSTAFPGINPLSSQLQGPLSTSLSGIGSGLGMPTVSSDVFSARKMSTPGLNPPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASIAHFLQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSLALAQAQAQSQPPKAPQPGQASTLVTTATTTTTAAKTTTITRPTAVGPKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMSQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFVKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILYTDLELKSLLLEAYVKGQQELLYVVPFVAKVLESSLRSVIFRPQNPWTMGIMNVLAELHQEHDLKLNLKFEIEVLCKNLSMDITDLKPGNLLRDKDKLKTLEEQLSAPKKETKPPEELLPIVTTDSVPFTAAPSTPATTTACTATGPPTPQFSYHDINVYALAGLAPHININVNIPLLQAHPQLKQCVRPAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESHMRVAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFAAALRAPTPQQREMMEEAAARIAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPMVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPSNDLSQPTGFLAQPMKQQAWPTDDVAHIYEKCISDLEQHLHAIPPALAMNPQTQAIRSLLEAVVMARNSRDGIAALGLLQKAVEGLLDATSGADPELLLSYRECHLLVLKALQDGRAYGPQWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKLLLVDERSVSHITEADLFHTIETLMRTSAHSRANAPEGLPQLMDVVRSNYEAMIDRHHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHPTTSPAIIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLIQDHDVRQTEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELNKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPSQFKKDLDSYLKTRSPVTFLSELRSNLQVSNEPGNRYNIQLINALVPYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANAEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWSHDFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	NM_001079951.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CCR4-NOT transcription complex, subunit 1 (cnot1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079951.2

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCTTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACTAAGAAAA ACTACAGAGC CAGCCAGCAG GAAATACAAC ATATTGTGAA TCGTCACGGC 
CCTGAGGCAG ACCGGCATCT ATTACGCTGT CTCTTTTCCC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCACCAG ACACAGTTTC TGATCCAGGA GTGCGTGTCT 
CTTATTACGA AACCAAATTT TATTTCAACG CTTTGCTACG CCATTGACAA TCCCCTGCAC 
TATCAAAAGA GTTTGAAACC GTCACCTCAT TTATTTACTC AGCTGAGTAA AGTTCTCAAG 
CTAAGCAAGG TTCAGGAGGT GATTTTGGGC CTTGCTCTGA GCAACTCCAG CAACGCAGAC 
CTTCGAGGGT TTGCTGCTCA GTTTGTGAAG CAGAAGCTCC CTGACCTCCT CCGCTCGTAC 
GTGGACGCCG ACCTCGGAGG GAATCAGGAA GGTGGCTTCC AGGACATTGC CATAGAGGTT 
CTGCATCTGC TCCTCTCCCA TCTTCTGTTC GGTCAAAAGG GCTCGAGCGG AGTCGGACAA 
GAGCAGATTG ACGCGTTCCT CAAAACACTG TGCAGAGATT TCCCGCAGGA GCGCTGTCCT 
GTGGTGCTTG CACCACTGCT GTATCCTGAC AAACGGGACA TTCTCATGGA CAGGATCCTG 
CCAGACTCTG GAGATTTAAA TAAGACCATG ATGGAGAGTT CTCTTGCTGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTCTT GAAGAGTGCA GGAACATAAT CCTGCAGTAT 
GGGGTGCGAG AGGTGACAGC CAGTCAGGTG GCCAGAGTGC TGGGCATGAT GGCCCGCACT 
CACTCAGGGC TGTCTGACGG CATCAGCCTT CAGACCATCA CCAATCCAGT GGGCGGTGGT 
GGGATCTGGA GTGACGGGAA GGACAAGAGT GACAGCTCTC AGGCCTGGAA TGTTGAAGTT 
CTAATTGATG TGGTCAAAGA AGTGAACCCT AATCTGAATT TCAAAGAGGT GACCTATGAG 
CTGGATCACC CAGGCTTCCT GATCCGGGAC AGTAAGGGGC TTCAGATAGT TGTTTATGGG 
ATCCAGAGGG GTCTGGGAAT GGAAGTGTTC CCGGTGGATC TCATTTACAG ACCATGGAAG 
CATGCAGAAG GACAGTTGTC GTTCATCCAG CACTCTCTAT TGAGTCCTGA AGTGTTCTGC 
TTTGCTGACA ACCCTTGCCA TACTGTTGCT ATTGACACCT TGAAGGCTCC ACCTGAGGAT 
GACAACAGGG AGATAGCCAC ATGGAAGAGC TTGGACCTTG TAGAGAGCCT CTTGCGCCTC 
TCTGAAGTTG GGCATTATGA ACAGGTGAAG CAGCTTTTTA GCTTCCCCAT CAAACACTGC 
CCGGACATGC TGGTGTTGGC GCTCCTGCAG ATCAGTACCT CCTGGCACAC CCTACGTCAT 
GAGCTCATCT CCACCCTCAT GCCCATCTTC CTGGGCAACC ACCCTAACTC TGCTATCATC 
TTGCACTATG CCTGGCATGG ACAGGGCCAG TCCCCATCCA TTCGCCAGCT GATCATGCAC 
TCTATGGCCG AGTGGTACAT GAGAGGGGAG CAATATGACC AGGCCAAGCT ATCTCGCATC 
CTGGATGTGG CTCAGGACTT GAAGTCTCTA TCGATGCTGC TGAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTAGCTGC ACTTGCCTCT CGCCGTGAAT ACCTCAAACT TGACAAATGG 
CTCACTGACA AAATACGGGA GCATGGGGAG CCATTCATCC AAGCGTGTGT CACATTTCTG 
AAGAGACGCT GCCCCTCTAT TATGGGTGGT TTGGCCCCTG AGAAAGACCA GCCGAAAAGT 
GCTCAACTTC CTCCTGAAAC ACTGGCTACC ATGCTTGCAT GTTTGCAGTC TTGTGCTGGG 
AGTGTGTCTC AAGAGCTGTC AGAGACAATC TTGACCATGG TGGCCAACTG TAGCAATGTG 
ATGAACAAGG CTCGGCAGCC GCCACCAGGA GTCCTGCCAA AGGGACGAGC GCCCAGCACC 
AGCAGTTTAG ATGCTATCTC ACCTGTGCAG ATGGACCCTC TCTCTGCAAT GGGCTCTTTA 
AGTCTAGGTG TCTCGTCCAC CTCTCACACC CCGAGCATGC AAGGATTCCC CAGCCTGCAG 
GGCTCTGCCT TCAGTAACCC CCAGTCCCCA GCCAAAGCCT TCTCAAACCT GCCCAACCCA 
AACCCCAGCA CAGCTTTCCC AGGAATCAAC CCCCTCTCTT CACAGCTCCA AGGTCCTCTG 
AGCACAAGCT TGTCTGGGAT TGGCTCAGGT TTGGGCATGC CCACCGTCAG CAGTGATGTC 
TTCAGCGCCA GGAAGATGAG CACACCGGGG CTAAACCCCC CAACCTTCCA GCAGACTGAC 
CTCTCTCAGG TGTGGCCCGA GGCAAACCAG CACTTTAGTA AGGAGATTGA CGATGAAGCT 
AACAGCTACT TCCAGCGCAT CTACAACCAT CCGCCACATC CCACAATGTC TGTGGACGAG 
GTGCTGGAGA TGCTGCAAAG ATTTAAGGAC TCAAACATCA AGCGGGAACG AGAAGTTTTT 
AACTGCATGC TGAGGAATCT GTTTGAAGAG TACCGATTCT TTCCTCAGTA TCCAGACAAA 
GAGCTGCACA TCACCGCCTG CCTCTTTGGG GGAATCATTG AGAAGGGCCT GGTCACATAC 
ATGGCGCTGG GCTTGGCCTT AAGATACGTG CTGGAAGCCT TACGCAAACC TTTCGGATCA 
AAGATGTATT ACTTTGGGAT CGCTGCACTA GATAGATTTA AAAATAGACT GAAGGACTAT 
CCCCAGTACT GTCAGCACTT GGCATCAATA GCCCATTTCC TACAGTTTCC ACACCATTTA 
CAAGAGTATA TAGAGTATGG CCAACAGTCA AGAGACCCTC CTGTGAAGAT GCAAGGCTCC 
ATTACCACCC CAGGCAGCCT GGCTCTAGCC CAAGCGCAAG CTCAGTCTCA GCCCCCTAAA 
GCCCCCCAAC CTGGCCAAGC CAGCACCTTA GTGACAACAG CCACCACGAC CACCACCGCT 
GCCAAAACCA CAACCATCAC AAGACCTACA GCTGTTGGAC CCAAGAAGGA TGTGCCACCT 
TCAATCAACA CCACAAACAT TGACACCTTG TTAGTGGCCA CAGATCAGAC CGAAAGGATC 
GTGGAGCCCC CAGAAAATGT TCAGGAGAAA ATTGCTTTTA TTTTCAATAA CTTGTCCCAG 
TCAAACATGT CCCAAAAGGT GGAGGAGCTA AAAGAGACTG TGAAAGAAGA ATTCATGCCC 
TGGGTTTCTC AGTACCTGGT AATGAAGCGT GTCAGCATTG AGCCCAATTT CCACAGCCTG 
TATTCTAACT TCTTGGACAC ACTGAAAAAC CCAGAGTTTG TCAAGATGGT TCTCAATGAG 
ACTTACAGAA ACATCAAGGT CCTCCTTACC TCGGATAAGG CAGCTGCTAA TTTCTCAGAT 
CGATCCCTGC TGAAGAATTT GGGTCACTGG CTTGGAATGA TCACCCTAGC AAAAAACAAA 
CCCATCCTGT ATACAGACCT GGAGCTGAAA TCTCTTTTGT TGGAGGCTTA TGTGAAAGGC 
CAGCAGGAGT TACTGTATGT TGTCCCTTTT GTGGCCAAAG TCTTGGAATC AAGTCTGCGG 
AGTGTGATCT TCAGACCGCA GAACCCTTGG ACAATGGGCA TCATGAATGT ACTGGCTGAA 
CTCCACCAGG AGCATGACTT AAAGCTTAAC CTCAAATTTG AGATTGAGGT ACTCTGCAAG 
AATCTGTCTA TGGACATCAC TGATCTTAAG CCAGGGAACC TGCTGAGAGA CAAAGATAAG 
CTGAAGACCT TGGAGGAGCA GCTCTCCGCA CCAAAGAAAG AGACCAAGCC TCCTGAAGAA 
TTGCTGCCTA TAGTTACAAC AGACTCTGTT CCTTTCACAG CTGCTCCCTC AACTCCGGCC 
ACCACCACGG CCTGTACAGC CACAGGGCCA CCTACACCTC AGTTCAGCTA TCATGACATC 
AATGTGTATG CATTGGCTGG ACTGGCCCCA CACATAAACA TCAATGTTAA CATCCCACTG 
CTACAGGCCC ACCCTCAGCT GAAGCAGTGC GTGAGACCAG CAATCGAACG TGCCGTACAG 
GAGCTTGTGC ACCCAGTGGT GGACCGATCC ATCAAGATCG CCATGACAAC CTGTGAGCAG 
ATAGTCAGGA AGGACTTTGC TCTAGACTCA GAGGAGTCTC ATATGCGTGT GGCTGCTCAC 
CACATGATGC GTAACCTGAC TGCCGGCATG GCCATGATCA CCTGCAGAGA GCCCCTGCTG 
ATGAGCATTG CCACCAATCT GAAGAACAGC TTTGCTGCAG CTCTTAGGGC TCCCACACCC 
CAGCAGAGAG AGATGATGGA GGAGGCGGCT GCTCGCATCG CACAGGACAA CTGTGAGCTT 
GCATGTTGCT TTATCCAGAA AACAGCAGTG GAGAAAGCTG GCCCGGAGAT GGATAAGAGA 
CTGGCAACTG AATTTGAGCT GAGGAAGCAT GCTCGCCAAG AAGGAAGACG CTACTGTGAC 
CCCATGGTGC TCACCTATCA GGCAGAGCGC ATGCCTGAAC AGATCAGACT GAAGGTTGGT 
GGAGTGGATC CTAAACAGCT GGCTGTTTAT GAGGAGTTCG CCCGCAACGT TCCAGGCTTC 
CTACCAAGTA ATGACCTGTC TCAACCAACT GGTTTTCTTG CTCAACCAAT GAAGCAACAA 
GCATGGCCCA CTGATGATGT GGCTCACATC TATGAGAAGT GCATTTCTGA CCTGGAACAG 
CACCTGCATG CAATTCCACC TGCACTGGCC ATGAACCCAC AGACCCAGGC CATACGTAGC 
CTGCTGGAGG CAGTTGTCAT GGCAAGGAAC TCCCGTGATG GCATTGCTGC CCTGGGACTT 
TTGCAGAAGG CTGTGGAGGG TCTTCTTGAT GCCACCAGTG GTGCTGATCC TGAATTGTTA 
CTAAGCTACA GGGAGTGCCA TCTGTTGGTG CTGAAAGCCT TACAGGATGG CCGTGCCTAC 
GGTCCACAGT GGTGCAACAA GCAAATCACC AGATGCCTGA TTGAGTGTAG AGATGAATAC 
AAGTACAATG TTGAGGCTGT GGAGCTTCTC ATCAGAAACC ACCTGGTCAA CATGCAGCAG 
TATGATTTGC ACCTAGCTCA GTCCATGGAG AATGGCCTCA ACTACATGGC AGTGGCATTT 
GCCATGCAGC TGGTGAAACT CTTGCTGGTT GATGAGCGCA GCGTGAGCCA CATCACTGAA 
GCAGATCTTT TCCACACTAT CGAGACACTG ATGAGGACCA GCGCCCACTC CAGAGCCAAC 
GCTCCTGAAG GTTTGCCACA GCTTATGGAT GTTGTCCGCT CCAACTATGA GGCCATGATT 
GATCGTCACC ATGGTGGACC AAATTTCATG ATGCACTCTG GAATTTCTCA AGCATCGGAG 
TACGATGACC CACCAGGCCT TAGAGAAAAG GCAGAGTACC TGCTGAGAGA ATGGGTCAAC 
CTGTACCACT CAGCAGCAGC AGGAAGGGAC AGCACCAAGG CCTTCTCTGC ATTTGTTGGC 
CAGATGCACC AGCAGGGTAT CCTAAAGACT GATGACTTGA TCACTCGCTT TTTCCGGCTA 
TGCACAGAAA TGTGTGTAGA GATCAGTTAT CGCGCTCAAG CTGAACAGCA GCACCCAACC 
ACCAGTCCTG CCATTATTAG GGCCAAATGC TACCATAACT TGGATGCTTT CGTTCGGCTT 
ATTGCCCTTC TGGTCAAACA CTCTGGAGAG GCCACCAACA CAGTCACTAA AATTAACCTG 
CTCAACAAGG TTTTGGGCAT TGTGGTGGGT GTGTTAATCC AGGATCATGA TGTGAGGCAG 
ACAGAGTTCC AGCAGCTTCC ATACCACAGA ATCTTCATCA TGCTCTTACT GGAGCTAAAC 
GCACCAGAGC ATGTGCTGGA GACCATCAAC TTCCAGACTC TCACTGCCTT CTGCAACACC 
TTCCACATCC TGAGGCCGAC TAAAGCACCT GGTTTTGTGT ATGCTTGGCT AGAGCTCATC 
TCTCACCGCA TCTTCATTGC CAGGATGCTT GCTCACACCC CACAACAGAA GGGTTGGCCT 
ATGTATGCCC AGCTGCTGAT AGACTTGTTT AAATATCTTG CACCCTTCCT AAGGAATGTT 
GAACTCAACA AACCTATGCA AATTCTCTAC AAGGGAACAC TTCGTGTGCT GCTTGTCCTG 
CTACATGACT TCCCAGAATT CCTGTGTGAC TACCATTACG GATTCTGTGA TGTCATCCCA 
CCCAACTGCA TCCAGCTGAG GAATCTGATC CTGAGTGCCT TCCCCCGCAA CATGAGGCTT 
CCGGACCCCT TCACCCCTAA TTTAAAGGTG GATATGCTGA GTGAGATCAA CATCGCTCCC 
CGCATCCTCA CAAACTTCAC TGGTGTGATG CCGTCCCAGT TTAAGAAGGA TCTTGACTCC 
TACCTCAAGA CCCGCTCTCC TGTCACCTTC CTCTCTGAGC TGCGCAGCAA CCTGCAGGTG 
TCCAATGAGC CAGGCAACCG CTACAACATT CAGCTGATCA ACGCTCTGGT GCCGTACGTT 
GGAACCCAGG CCATTGCCCA CATTCACAAC AAGGGCAGCA CTCCCTCCAT GAGCACCATC 
ACACACTCGG CACACATGGA CATCTTCCAG AACCTCGCAG TCGATCTAGA CACTGAGGGT 
CGCTATCTGT TCCTGAATGC GATAGCCAAT CAGCTGCGTT ATCCAAACAG CCACACGCAC 
TACTTCAGCT GTACCATGCT GTATCTGTTT GCCGAGGCCA ATGCTGAAGC CATTCAAGAG 
CAGATCACCA GGGTCTTGCT GGAGAGGCTG ATTGTGAACA GGCCACACCC GTGGGGTCTG 
CTCATCACCT TCATAGAGCT GATCAAGAAC CCTGCCTTCA AGTTCTGGAG CCATGATTTT 
GTGCACTGCG CCCCAGAGAT CGAGAAGTTG TTCCAGTCAG TGGCTCAGTG CTGTATGGGG 
CAGAAGCAGG CCCAGCAGGT GATGGAGGGA ACCGGTGCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Large-scale discovery of novel genetic causes of developmental disorders.
Nature519(7542)223-8(2015 Mar)

Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA).
Nature methods8(1)67-9(2011 Jan)
Sander JD,Dahlborg EJ,Goodwin MJ,Cade L,Zhang F,Cifuentes D,Curtin SJ,Blackburn JS,Thibodeau-Beganny S,Qi Y,Pierick CJ,Hoffman E,Maeder ML,Khayter C,Reyon D,Dobbs D,Langenau DM,Stupar RM,Giraldez AJ,Voytas DF,Peterson RT,Yeh JR,Joung JK

Identification of 315 genes essential for early zebrafish development.
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)
Amsterdam A,Nissen RM,Sun Z,Swindell EC,Farrington S,Hopkins N

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cnot1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

ORF Insert Sequence:

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