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HCN4L cDNA ORF clone, Danio rerio(Zebrafish)

Summary

Gene Symbol HCN4L
Entrez Gene ID 556759
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 4l
Synonyms si:dkeyp-74b4.2
General protein information
Preferred Names

hyperpolarization activated cyclic nucleotide-gated potassium channel 4l

Names

potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3-like
hcn3
Gene Type protein-coding
Organism Danio rerio(Zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_679638.8 XP_684730.5 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3-like
XM_679638.7 XP_684730.5 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3-like

Pathways from BioSystems


Homologies


HCN4 XP_002804905.1
Mus musculus (house mouse) Hcn4 NP_001074661.1
Danio rerio (zebrafish) hcn4 XP_686078.4
Xenopus tropicalis (tropical clawed frog) hcn4 XP_004916024.1
Pan troglodytes (chimpanzee) HCN4 XP_003952750.1
Bos taurus (cattle) HCN4 XP_002690539.1
Gallus gallus (chicken) HCN4 XP_425050.3
Homo sapiens (human) HCN4 NP_005468.1
Danio rerio (zebrafish) hcn4l XP_684730.5
Rattus norvegicus (Norway rat) Hcn4 NP_067690.1

Gene Ontology

The following HCN4L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCN4L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa29570 XM_679638.8
Latest version!		 Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4l (hcn4l), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa29570 XM_679638.7 Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4l (hcn4l), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa29570
Clone ID Related Accession (Same CDS sequence) XM_679638.8 , XM_679638.7
Accession Version XM_679638.8 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3-like
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_679638.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGCATAGGG TGCATTCTTC CATGCGCAAA CGGCTCTATA GCTTGCCACA TCAGTTTGGA 
CACAAGGGTG CTATGACTCG AGACGGCGAA GATAGCGACA AGGACACTCG GAGGAAAAGT 
GTCAAGATGA AGCATTTTCC TTCACCTTCC TCCACGGGGA GCTCGAAAGG TGTCTGCGAG 
GCCAAGAGCG GGGACGCGGA GACCGATGTG GGGAGACCCT TCAGAACCAA CGTGAACGGC 
GACTGCCGGA GGTTCAGGGG CAGCCTATCG TCCCTCACCA GTCGGCATGC TGCTCCAGAC 
GCGGATCTGG CCGAGCAAAG GCGTCTTATC GGGGATGCGA GCCCGGAGGA CGACGGCCCC 
TCGGAGCAAC CGGGACCCGG AGAGAGCCCG GGCGCGAAGG GCGGTCGCGG CGATGGGGCA 
CAACAGGCGC CCTCTGAAGA GCAGTCGACT TTCGTAAAGT TGGAGGGTAT CGATCAGATC 
CTGCCGGACG ATGAGAGGAT GTACCAGGCG GGCTTCATGC ACAGGCAGTT TGGAGCGATG 
CTTCAGCCCG GGGTCAACAA GTTCTCGCTT CGAATGTTCG GGAGCGAGAA GGCAGTGGAG 
CACGAGCAAG AACGGGTCAA ATCTGCAGGC TTTTGGATCA TCCACCCGTA CAGTGACTTC 
AGGTTTTACT GGGACCTGAT CATGCTTCTT CTAATGGTGG GCAACCTGAT CATCATTCCC 
GTGGGCATCA CCTTCTTCAA AGACGAACAC ACACCCGCCT GGATAGTGTT TAACGTGGTC 
TCCGACACCT TCTTCCTGGT CGACCTGGTG CTGAACTTCC GCACGGGGAT TGTGAAAGAG 
GACAGTACAG AAATCATCCT GGACCCTCAG CAGATCAAGG TCAGGTACTT GCGCAGCTGG 
TTTGCCGTGG ATTTCCTCTC CTCCATCCCT GTGGATTACA TCTTCCTCAT TGTGGAGACA 
CGCATCGACT CGGATTTCTA CAAGACGGCA AGAGCGTTGA GGATTGTGCG TTTCACCAAA 
ATACTGAGTC TGCTTCGGTT GTTGAGATTG TCCAGGCTCA TTAGATACAT CCACCAATGG 
GAGGAGATTT TTCACATGAC CTATGACCTG GCTAGTGCCA TGGTGCGCAT TGTAAATCTC 
ATTGGGATGA TGTTGTTGCT GTGCCACTGG GACGGGTGTT TGCAGTTCCT GGTGCCCATG 
TTGCAGGATT TCCCTTCAGA CTGCTGGGTG GCCAAAAACA AAATGGTGAA CGACACATGG 
GGTCAGCAGT ACTCTTATGC CCTGTTTAAG GCCATGAGTC ACATGCTGTG TATCGGGTAT 
GGCATGTACC CTCCTGTGGG CATGACGGAT GTGTGGTTGA CCATCCTCAG CATGATCGTG 
GGCGCAACCT GCTATGCCAT GTTTGTAGGC CACGCCACCG CCCTCATTCA GTCACTGGAC 
TCATCACGCA GACAGTACCA GGAGAAGTAC AAGCAAGTGG AGCAGTACAT GTCCTTCCAC 
AAACTTCCAG CAGACATGCG GCAAAGGATC CATGATTATT ATGAGCATCG CTATCAGGGC 
AAGATGTTTG ATGAGGAGAG CATTCTGGGA GAACTGAACG AGCCGCTCAG AGAGGAAATA 
ATCAGCTTTA ACTGTCGAAA GCTTGTTGCC ACAATGCCGT TGTTCGCCAA TGCGGATCCA 
AATTTTGTGA CCTCCATGCT CACCAAGCTG CGTTTCGAGG TGTTCCAGCC AGGAGATTAC 
ATTATCAGAG AAGGGACCGT CGGCAAGAAG ATGTACTTTA TACAACACGG TGTACTAACT 
GTCCTGACGA AGGGCAGCAA AGAGACAAAG ATCTCGGACG GGTCTTATTT TGGAGAGATC 
TGCTTGCTGA CTCGAGGCAG AAGAACGGCC AGTGTGAGAG CGGACACATA TTGCCGTCTG 
TACTCGCTCT CTGTGGACCA TTTTAACGAG GTGTTGGAGG AGTACCCCAT GATGCGACGA 
GCATTTGAGA CCGTAGCCCT CGATCGCCTT GACCGCATAG GAAAGAGGAA CTCTGTACTC 
CAGCACAAAG TCCAACGGGA TCTTAATTCT GGAGTTTTGA ACTACCAAGA AAATGAGATC 
ATCCAGCAGA TCGTGCAGCA TGACAGGGAT ATGGCCCACT GTGCACACCT CATGCAGACA 
CCTCCCCCAC CTCCTCATCC AGCACCGACT CCATCCTCCC ACACCCCTGT CATATGGGCA 
CCTCTTATTC AGGCTCCTCT GCAAGCTGCT GCAGCCACTA CTTCAGTTGC CATCGCCCTG 
ACTCGTCACC CCCATTTGCC CCACACCCTC TTCCGACCTC CTGTTCCTCG GCTTGGCTCA 
CTGAAAGACC AGCCAGGCTA TTTTAAAAGG TTTCCAACTG CACTTTCGGG TGCTGATACA 
TTTGGTGGCT CCTCATCAAC CAGCTCGCAG TTCAGCTCTT GTATAGAAAC CCCAATTCTG 
GACACACTCA GGGCCGAGAG ATCATCTACC TCCACACCGC CACCATCCTC CAGCTCTCTG 
CCATCCACCA TAACAACAAC TGTCACCACT ACCACTTCAA GCATCACAGA GTCTTCCGTC 
TACCACCATA GACCCATTAT CTCTCATGGA TCCAAAGACT TTAGCGTTGC CCAACTTCAT 
TCTCAACCGC AACTTTCTCA GGCCTCTTTC TCAAGCATTG CTGCATCACT TCCTGGATTC 
TTCCAACAAG GAGCAGCTGG AGGAGAGGCT GTTCATCTAG CGGTTCCCCC TGCATCCACC 
CTTACTCCTT TGATAGCGCA CTCTGTAAGT CCCATCCTGA CCCAGCTACA TGCAACTCAT 
TCAAATCCTC AACCAACAAA ACCAATTCAA GACTTAAATA AAGCTGGAAG GACAGCCCCC 
CAATCTCCAG TAACAGAGTC CCCAGTCCAC TCCAAGAGCA TTCTTGAACA AGTACAGAGT 
CCAACAAGTG TCCAGTCCCA GCATCCTCAG ATTGGTATAC AGCCGGATGT TCTTTCCCAG 
CTTGCTCAAG AACCATCTGC TCTTGCTTCC CTTGCACAAT ATGGATCTGG AAATGCGTCC 
CCATGTAGCA CACCTTCAGC TTGGAGCCCC ACGTGCCAGA GTCCAACAGC AGAGAAAATG 
AGAATGTCAG CGCATAGCCA ACCTTCTAGC ATCTCTGGAT CCCAAACGTC ACTACTGACC 
CCTCAGACAC TCTTTCCACC ACCACCTCAA CCAAGAGAAA GGGGTGGATC TCTAGTTGAT 
CTACCTTCAC AAGACTTAAG CACCCTTTCC ATGTCCCTTC CTATTCCTGG ACTAGCCGAA 
AAATCCAGGG CTGTGGGAAG AGGACCACAA CCACCAGACT TATCTCATCA CCGAGGATCC 
ACTTCAGGTT ACTCACCACT CCCATCCCCC CCACCCCTTG TGAATCCATT TAGCTCAATA 
CCTGGTCATG TTGTCTTATC GAGACAAACT TCCAAGTTGT CCATAACTCA GACTGGGTCC 
TCAGGTGGAT CACCAGCCCA TGGGTCAAGT GACAGGTCCA CCCCTGGGAC ACCAAGCTTG 
CACCCCAAAC TCCCATCAAA CTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_684730.5
CDS1444..5010
Translation

Target ORF information:

RefSeq Version XM_679638.8
Organism Danio rerio(zebrafish)
Definition Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4l (hcn4l), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_679638.8

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGCATAGGG TGCATTCTTC CATGCGCAAA CGGCTCTATA GCTTGCCACA TCAGTTTGGA 
CACAAGGGTG CTATGACTCG AGACGGCGAA GATAGCGACA AGGACACTCG GAGGAAAAGT 
GTCAAGATGA AGCATTTTCC TTCACCTTCC TCCACGGGGA GCTCGAAAGG TGTCTGCGAG 
GCCAAGAGCG GGGACGCGGA GACCGATGTG GGGAGACCCT TCAGAACCAA CGTGAACGGC 
GACTGCCGGA GGTTCAGGGG CAGCCTATCG TCCCTCACCA GTCGGCATGC TGCTCCAGAC 
GCGGATCTGG CCGAGCAAAG GCGTCTTATC GGGGATGCGA GCCCGGAGGA CGACGGCCCC 
TCGGAGCAAC CGGGACCCGG AGAGAGCCCG GGCGCGAAGG GCGGTCGCGG CGATGGGGCA 
CAACAGGCGC CCTCTGAAGA GCAGTCGACT TTCGTAAAGT TGGAGGGTAT CGATCAGATC 
CTGCCGGACG ATGAGAGGAT GTACCAGGCG GGCTTCATGC ACAGGCAGTT TGGAGCGATG 
CTTCAGCCCG GGGTCAACAA GTTCTCGCTT CGAATGTTCG GGAGCGAGAA GGCAGTGGAG 
CACGAGCAAG AACGGGTCAA ATCTGCAGGC TTTTGGATCA TCCACCCGTA CAGTGACTTC 
AGGTTTTACT GGGACCTGAT CATGCTTCTT CTAATGGTGG GCAACCTGAT CATCATTCCC 
GTGGGCATCA CCTTCTTCAA AGACGAACAC ACACCCGCCT GGATAGTGTT TAACGTGGTC 
TCCGACACCT TCTTCCTGGT CGACCTGGTG CTGAACTTCC GCACGGGGAT TGTGAAAGAG 
GACAGTACAG AAATCATCCT GGACCCTCAG CAGATCAAGG TCAGGTACTT GCGCAGCTGG 
TTTGCCGTGG ATTTCCTCTC CTCCATCCCT GTGGATTACA TCTTCCTCAT TGTGGAGACA 
CGCATCGACT CGGATTTCTA CAAGACGGCA AGAGCGTTGA GGATTGTGCG TTTCACCAAA 
ATACTGAGTC TGCTTCGGTT GTTGAGATTG TCCAGGCTCA TTAGATACAT CCACCAATGG 
GAGGAGATTT TTCACATGAC CTATGACCTG GCTAGTGCCA TGGTGCGCAT TGTAAATCTC 
ATTGGGATGA TGTTGTTGCT GTGCCACTGG GACGGGTGTT TGCAGTTCCT GGTGCCCATG 
TTGCAGGATT TCCCTTCAGA CTGCTGGGTG GCCAAAAACA AAATGGTGAA CGACACATGG 
GGTCAGCAGT ACTCTTATGC CCTGTTTAAG GCCATGAGTC ACATGCTGTG TATCGGGTAT 
GGCATGTACC CTCCTGTGGG CATGACGGAT GTGTGGTTGA CCATCCTCAG CATGATCGTG 
GGCGCAACCT GCTATGCCAT GTTTGTAGGC CACGCCACCG CCCTCATTCA GTCACTGGAC 
TCATCACGCA GACAGTACCA GGAGAAGTAC AAGCAAGTGG AGCAGTACAT GTCCTTCCAC 
AAACTTCCAG CAGACATGCG GCAAAGGATC CATGATTATT ATGAGCATCG CTATCAGGGC 
AAGATGTTTG ATGAGGAGAG CATTCTGGGA GAACTGAACG AGCCGCTCAG AGAGGAAATA 
ATCAGCTTTA ACTGTCGAAA GCTTGTTGCC ACAATGCCGT TGTTCGCCAA TGCGGATCCA 
AATTTTGTGA CCTCCATGCT CACCAAGCTG CGTTTCGAGG TGTTCCAGCC AGGAGATTAC 
ATTATCAGAG AAGGGACCGT CGGCAAGAAG ATGTACTTTA TACAACACGG TGTACTAACT 
GTCCTGACGA AGGGCAGCAA AGAGACAAAG ATCTCGGACG GGTCTTATTT TGGAGAGATC 
TGCTTGCTGA CTCGAGGCAG AAGAACGGCC AGTGTGAGAG CGGACACATA TTGCCGTCTG 
TACTCGCTCT CTGTGGACCA TTTTAACGAG GTGTTGGAGG AGTACCCCAT GATGCGACGA 
GCATTTGAGA CCGTAGCCCT CGATCGCCTT GACCGCATAG GAAAGAGGAA CTCTGTACTC 
CAGCACAAAG TCCAACGGGA TCTTAATTCT GGAGTTTTGA ACTACCAAGA AAATGAGATC 
ATCCAGCAGA TCGTGCAGCA TGACAGGGAT ATGGCCCACT GTGCACACCT CATGCAGACA 
CCTCCCCCAC CTCCTCATCC AGCACCGACT CCATCCTCCC ACACCCCTGT CATATGGGCA 
CCTCTTATTC AGGCTCCTCT GCAAGCTGCT GCAGCCACTA CTTCAGTTGC CATCGCCCTG 
ACTCGTCACC CCCATTTGCC CCACACCCTC TTCCGACCTC CTGTTCCTCG GCTTGGCTCA 
CTGAAAGACC AGCCAGGCTA TTTTAAAAGG TTTCCAACTG CACTTTCGGG TGCTGATACA 
TTTGGTGGCT CCTCATCAAC CAGCTCGCAG TTCAGCTCTT GTATAGAAAC CCCAATTCTG 
GACACACTCA GGGCCGAGAG ATCATCTACC TCCACACCGC CACCATCCTC CAGCTCTCTG 
CCATCCACCA TAACAACAAC TGTCACCACT ACCACTTCAA GCATCACAGA GTCTTCCGTC 
TACCACCATA GACCCATTAT CTCTCATGGA TCCAAAGACT TTAGCGTTGC CCAACTTCAT 
TCTCAACCGC AACTTTCTCA GGCCTCTTTC TCAAGCATTG CTGCATCACT TCCTGGATTC 
TTCCAACAAG GAGCAGCTGG AGGAGAGGCT GTTCATCTAG CGGTTCCCCC TGCATCCACC 
CTTACTCCTT TGATAGCGCA CTCTGTAAGT CCCATCCTGA CCCAGCTACA TGCAACTCAT 
TCAAATCCTC AACCAACAAA ACCAATTCAA GACTTAAATA AAGCTGGAAG GACAGCCCCC 
CAATCTCCAG TAACAGAGTC CCCAGTCCAC TCCAAGAGCA TTCTTGAACA AGTACAGAGT 
CCAACAAGTG TCCAGTCCCA GCATCCTCAG ATTGGTATAC AGCCGGATGT TCTTTCCCAG 
CTTGCTCAAG AACCATCTGC TCTTGCTTCC CTTGCACAAT ATGGATCTGG AAATGCGTCC 
CCATGTAGCA CACCTTCAGC TTGGAGCCCC ACGTGCCAGA GTCCAACAGC AGAGAAAATG 
AGAATGTCAG CGCATAGCCA ACCTTCTAGC ATCTCTGGAT CCCAAACGTC ACTACTGACC 
CCTCAGACAC TCTTTCCACC ACCACCTCAA CCAAGAGAAA GGGGTGGATC TCTAGTTGAT 
CTACCTTCAC AAGACTTAAG CACCCTTTCC ATGTCCCTTC CTATTCCTGG ACTAGCCGAA 
AAATCCAGGG CTGTGGGAAG AGGACCACAA CCACCAGACT TATCTCATCA CCGAGGATCC 
ACTTCAGGTT ACTCACCACT CCCATCCCCC CCACCCCTTG TGAATCCATT TAGCTCAATA 
CCTGGTCATG TTGTCTTATC GAGACAAACT TCCAAGTTGT CCATAACTCA GACTGGGTCC 
TCAGGTGGAT CACCAGCCCA TGGGTCAAGT GACAGGTCCA CCCCTGGGAC ACCAAGCTTG 
CACCCCAAAC TCCCATCAAA CTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa29570
Clone ID Related Accession (Same CDS sequence) XM_679638.8 , XM_679638.7
Accession Version XM_679638.7 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3-like
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_679638.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGCATAGGG TGCATTCTTC CATGCGCAAA CGGCTCTATA GCTTGCCACA TCAGTTTGGA 
CACAAGGGTG CTATGACTCG AGACGGCGAA GATAGCGACA AGGACACTCG GAGGAAAAGT 
GTCAAGATGA AGCATTTTCC TTCACCTTCC TCCACGGGGA GCTCGAAAGG TGTCTGCGAG 
GCCAAGAGCG GGGACGCGGA GACCGATGTG GGGAGACCCT TCAGAACCAA CGTGAACGGC 
GACTGCCGGA GGTTCAGGGG CAGCCTATCG TCCCTCACCA GTCGGCATGC TGCTCCAGAC 
GCGGATCTGG CCGAGCAAAG GCGTCTTATC GGGGATGCGA GCCCGGAGGA CGACGGCCCC 
TCGGAGCAAC CGGGACCCGG AGAGAGCCCG GGCGCGAAGG GCGGTCGCGG CGATGGGGCA 
CAACAGGCGC CCTCTGAAGA GCAGTCGACT TTCGTAAAGT TGGAGGGTAT CGATCAGATC 
CTGCCGGACG ATGAGAGGAT GTACCAGGCG GGCTTCATGC ACAGGCAGTT TGGAGCGATG 
CTTCAGCCCG GGGTCAACAA GTTCTCGCTT CGAATGTTCG GGAGCGAGAA GGCAGTGGAG 
CACGAGCAAG AACGGGTCAA ATCTGCAGGC TTTTGGATCA TCCACCCGTA CAGTGACTTC 
AGGTTTTACT GGGACCTGAT CATGCTTCTT CTAATGGTGG GCAACCTGAT CATCATTCCC 
GTGGGCATCA CCTTCTTCAA AGACGAACAC ACACCCGCCT GGATAGTGTT TAACGTGGTC 
TCCGACACCT TCTTCCTGGT CGACCTGGTG CTGAACTTCC GCACGGGGAT TGTGAAAGAG 
GACAGTACAG AAATCATCCT GGACCCTCAG CAGATCAAGG TCAGGTACTT GCGCAGCTGG 
TTTGCCGTGG ATTTCCTCTC CTCCATCCCT GTGGATTACA TCTTCCTCAT TGTGGAGACA 
CGCATCGACT CGGATTTCTA CAAGACGGCA AGAGCGTTGA GGATTGTGCG TTTCACCAAA 
ATACTGAGTC TGCTTCGGTT GTTGAGATTG TCCAGGCTCA TTAGATACAT CCACCAATGG 
GAGGAGATTT TTCACATGAC CTATGACCTG GCTAGTGCCA TGGTGCGCAT TGTAAATCTC 
ATTGGGATGA TGTTGTTGCT GTGCCACTGG GACGGGTGTT TGCAGTTCCT GGTGCCCATG 
TTGCAGGATT TCCCTTCAGA CTGCTGGGTG GCCAAAAACA AAATGGTGAA CGACACATGG 
GGTCAGCAGT ACTCTTATGC CCTGTTTAAG GCCATGAGTC ACATGCTGTG TATCGGGTAT 
GGCATGTACC CTCCTGTGGG CATGACGGAT GTGTGGTTGA CCATCCTCAG CATGATCGTG 
GGCGCAACCT GCTATGCCAT GTTTGTAGGC CACGCCACCG CCCTCATTCA GTCACTGGAC 
TCATCACGCA GACAGTACCA GGAGAAGTAC AAGCAAGTGG AGCAGTACAT GTCCTTCCAC 
AAACTTCCAG CAGACATGCG GCAAAGGATC CATGATTATT ATGAGCATCG CTATCAGGGC 
AAGATGTTTG ATGAGGAGAG CATTCTGGGA GAACTGAACG AGCCGCTCAG AGAGGAAATA 
ATCAGCTTTA ACTGTCGAAA GCTTGTTGCC ACAATGCCGT TGTTCGCCAA TGCGGATCCA 
AATTTTGTGA CCTCCATGCT CACCAAGCTG CGTTTCGAGG TGTTCCAGCC AGGAGATTAC 
ATTATCAGAG AAGGGACCGT CGGCAAGAAG ATGTACTTTA TACAACACGG TGTACTAACT 
GTCCTGACGA AGGGCAGCAA AGAGACAAAG ATCTCGGACG GGTCTTATTT TGGAGAGATC 
TGCTTGCTGA CTCGAGGCAG AAGAACGGCC AGTGTGAGAG CGGACACATA TTGCCGTCTG 
TACTCGCTCT CTGTGGACCA TTTTAACGAG GTGTTGGAGG AGTACCCCAT GATGCGACGA 
GCATTTGAGA CCGTAGCCCT CGATCGCCTT GACCGCATAG GAAAGAGGAA CTCTGTACTC 
CAGCACAAAG TCCAACGGGA TCTTAATTCT GGAGTTTTGA ACTACCAAGA AAATGAGATC 
ATCCAGCAGA TCGTGCAGCA TGACAGGGAT ATGGCCCACT GTGCACACCT CATGCAGACA 
CCTCCCCCAC CTCCTCATCC AGCACCGACT CCATCCTCCC ACACCCCTGT CATATGGGCA 
CCTCTTATTC AGGCTCCTCT GCAAGCTGCT GCAGCCACTA CTTCAGTTGC CATCGCCCTG 
ACTCGTCACC CCCATTTGCC CCACACCCTC TTCCGACCTC CTGTTCCTCG GCTTGGCTCA 
CTGAAAGACC AGCCAGGCTA TTTTAAAAGG TTTCCAACTG CACTTTCGGG TGCTGATACA 
TTTGGTGGCT CCTCATCAAC CAGCTCGCAG TTCAGCTCTT GTATAGAAAC CCCAATTCTG 
GACACACTCA GGGCCGAGAG ATCATCTACC TCCACACCGC CACCATCCTC CAGCTCTCTG 
CCATCCACCA TAACAACAAC TGTCACCACT ACCACTTCAA GCATCACAGA GTCTTCCGTC 
TACCACCATA GACCCATTAT CTCTCATGGA TCCAAAGACT TTAGCGTTGC CCAACTTCAT 
TCTCAACCGC AACTTTCTCA GGCCTCTTTC TCAAGCATTG CTGCATCACT TCCTGGATTC 
TTCCAACAAG GAGCAGCTGG AGGAGAGGCT GTTCATCTAG CGGTTCCCCC TGCATCCACC 
CTTACTCCTT TGATAGCGCA CTCTGTAAGT CCCATCCTGA CCCAGCTACA TGCAACTCAT 
TCAAATCCTC AACCAACAAA ACCAATTCAA GACTTAAATA AAGCTGGAAG GACAGCCCCC 
CAATCTCCAG TAACAGAGTC CCCAGTCCAC TCCAAGAGCA TTCTTGAACA AGTACAGAGT 
CCAACAAGTG TCCAGTCCCA GCATCCTCAG ATTGGTATAC AGCCGGATGT TCTTTCCCAG 
CTTGCTCAAG AACCATCTGC TCTTGCTTCC CTTGCACAAT ATGGATCTGG AAATGCGTCC 
CCATGTAGCA CACCTTCAGC TTGGAGCCCC ACGTGCCAGA GTCCAACAGC AGAGAAAATG 
AGAATGTCAG CGCATAGCCA ACCTTCTAGC ATCTCTGGAT CCCAAACGTC ACTACTGACC 
CCTCAGACAC TCTTTCCACC ACCACCTCAA CCAAGAGAAA GGGGTGGATC TCTAGTTGAT 
CTACCTTCAC AAGACTTAAG CACCCTTTCC ATGTCCCTTC CTATTCCTGG ACTAGCCGAA 
AAATCCAGGG CTGTGGGAAG AGGACCACAA CCACCAGACT TATCTCATCA CCGAGGATCC 
ACTTCAGGTT ACTCACCACT CCCATCCCCC CCACCCCTTG TGAATCCATT TAGCTCAATA 
CCTGGTCATG TTGTCTTATC GAGACAAACT TCCAAGTTGT CCATAACTCA GACTGGGTCC 
TCAGGTGGAT CACCAGCCCA TGGGTCAAGT GACAGGTCCA CCCCTGGGAC ACCAAGCTTG 
CACCCCAAAC TCCCATCAAA CTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_684730.5
CDS1444..5010
Translation

Target ORF information:

RefSeq Version XM_679638.7
Organism Danio rerio(Zebrafish)
Definition Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4l (hcn4l), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_679638.7

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGCATAGGG TGCATTCTTC CATGCGCAAA CGGCTCTATA GCTTGCCACA TCAGTTTGGA 
CACAAGGGTG CTATGACTCG AGACGGCGAA GATAGCGACA AGGACACTCG GAGGAAAAGT 
GTCAAGATGA AGCATTTTCC TTCACCTTCC TCCACGGGGA GCTCGAAAGG TGTCTGCGAG 
GCCAAGAGCG GGGACGCGGA GACCGATGTG GGGAGACCCT TCAGAACCAA CGTGAACGGC 
GACTGCCGGA GGTTCAGGGG CAGCCTATCG TCCCTCACCA GTCGGCATGC TGCTCCAGAC 
GCGGATCTGG CCGAGCAAAG GCGTCTTATC GGGGATGCGA GCCCGGAGGA CGACGGCCCC 
TCGGAGCAAC CGGGACCCGG AGAGAGCCCG GGCGCGAAGG GCGGTCGCGG CGATGGGGCA 
CAACAGGCGC CCTCTGAAGA GCAGTCGACT TTCGTAAAGT TGGAGGGTAT CGATCAGATC 
CTGCCGGACG ATGAGAGGAT GTACCAGGCG GGCTTCATGC ACAGGCAGTT TGGAGCGATG 
CTTCAGCCCG GGGTCAACAA GTTCTCGCTT CGAATGTTCG GGAGCGAGAA GGCAGTGGAG 
CACGAGCAAG AACGGGTCAA ATCTGCAGGC TTTTGGATCA TCCACCCGTA CAGTGACTTC 
AGGTTTTACT GGGACCTGAT CATGCTTCTT CTAATGGTGG GCAACCTGAT CATCATTCCC 
GTGGGCATCA CCTTCTTCAA AGACGAACAC ACACCCGCCT GGATAGTGTT TAACGTGGTC 
TCCGACACCT TCTTCCTGGT CGACCTGGTG CTGAACTTCC GCACGGGGAT TGTGAAAGAG 
GACAGTACAG AAATCATCCT GGACCCTCAG CAGATCAAGG TCAGGTACTT GCGCAGCTGG 
TTTGCCGTGG ATTTCCTCTC CTCCATCCCT GTGGATTACA TCTTCCTCAT TGTGGAGACA 
CGCATCGACT CGGATTTCTA CAAGACGGCA AGAGCGTTGA GGATTGTGCG TTTCACCAAA 
ATACTGAGTC TGCTTCGGTT GTTGAGATTG TCCAGGCTCA TTAGATACAT CCACCAATGG 
GAGGAGATTT TTCACATGAC CTATGACCTG GCTAGTGCCA TGGTGCGCAT TGTAAATCTC 
ATTGGGATGA TGTTGTTGCT GTGCCACTGG GACGGGTGTT TGCAGTTCCT GGTGCCCATG 
TTGCAGGATT TCCCTTCAGA CTGCTGGGTG GCCAAAAACA AAATGGTGAA CGACACATGG 
GGTCAGCAGT ACTCTTATGC CCTGTTTAAG GCCATGAGTC ACATGCTGTG TATCGGGTAT 
GGCATGTACC CTCCTGTGGG CATGACGGAT GTGTGGTTGA CCATCCTCAG CATGATCGTG 
GGCGCAACCT GCTATGCCAT GTTTGTAGGC CACGCCACCG CCCTCATTCA GTCACTGGAC 
TCATCACGCA GACAGTACCA GGAGAAGTAC AAGCAAGTGG AGCAGTACAT GTCCTTCCAC 
AAACTTCCAG CAGACATGCG GCAAAGGATC CATGATTATT ATGAGCATCG CTATCAGGGC 
AAGATGTTTG ATGAGGAGAG CATTCTGGGA GAACTGAACG AGCCGCTCAG AGAGGAAATA 
ATCAGCTTTA ACTGTCGAAA GCTTGTTGCC ACAATGCCGT TGTTCGCCAA TGCGGATCCA 
AATTTTGTGA CCTCCATGCT CACCAAGCTG CGTTTCGAGG TGTTCCAGCC AGGAGATTAC 
ATTATCAGAG AAGGGACCGT CGGCAAGAAG ATGTACTTTA TACAACACGG TGTACTAACT 
GTCCTGACGA AGGGCAGCAA AGAGACAAAG ATCTCGGACG GGTCTTATTT TGGAGAGATC 
TGCTTGCTGA CTCGAGGCAG AAGAACGGCC AGTGTGAGAG CGGACACATA TTGCCGTCTG 
TACTCGCTCT CTGTGGACCA TTTTAACGAG GTGTTGGAGG AGTACCCCAT GATGCGACGA 
GCATTTGAGA CCGTAGCCCT CGATCGCCTT GACCGCATAG GAAAGAGGAA CTCTGTACTC 
CAGCACAAAG TCCAACGGGA TCTTAATTCT GGAGTTTTGA ACTACCAAGA AAATGAGATC 
ATCCAGCAGA TCGTGCAGCA TGACAGGGAT ATGGCCCACT GTGCACACCT CATGCAGACA 
CCTCCCCCAC CTCCTCATCC AGCACCGACT CCATCCTCCC ACACCCCTGT CATATGGGCA 
CCTCTTATTC AGGCTCCTCT GCAAGCTGCT GCAGCCACTA CTTCAGTTGC CATCGCCCTG 
ACTCGTCACC CCCATTTGCC CCACACCCTC TTCCGACCTC CTGTTCCTCG GCTTGGCTCA 
CTGAAAGACC AGCCAGGCTA TTTTAAAAGG TTTCCAACTG CACTTTCGGG TGCTGATACA 
TTTGGTGGCT CCTCATCAAC CAGCTCGCAG TTCAGCTCTT GTATAGAAAC CCCAATTCTG 
GACACACTCA GGGCCGAGAG ATCATCTACC TCCACACCGC CACCATCCTC CAGCTCTCTG 
CCATCCACCA TAACAACAAC TGTCACCACT ACCACTTCAA GCATCACAGA GTCTTCCGTC 
TACCACCATA GACCCATTAT CTCTCATGGA TCCAAAGACT TTAGCGTTGC CCAACTTCAT 
TCTCAACCGC AACTTTCTCA GGCCTCTTTC TCAAGCATTG CTGCATCACT TCCTGGATTC 
TTCCAACAAG GAGCAGCTGG AGGAGAGGCT GTTCATCTAG CGGTTCCCCC TGCATCCACC 
CTTACTCCTT TGATAGCGCA CTCTGTAAGT CCCATCCTGA CCCAGCTACA TGCAACTCAT 
TCAAATCCTC AACCAACAAA ACCAATTCAA GACTTAAATA AAGCTGGAAG GACAGCCCCC 
CAATCTCCAG TAACAGAGTC CCCAGTCCAC TCCAAGAGCA TTCTTGAACA AGTACAGAGT 
CCAACAAGTG TCCAGTCCCA GCATCCTCAG ATTGGTATAC AGCCGGATGT TCTTTCCCAG 
CTTGCTCAAG AACCATCTGC TCTTGCTTCC CTTGCACAAT ATGGATCTGG AAATGCGTCC 
CCATGTAGCA CACCTTCAGC TTGGAGCCCC ACGTGCCAGA GTCCAACAGC AGAGAAAATG 
AGAATGTCAG CGCATAGCCA ACCTTCTAGC ATCTCTGGAT CCCAAACGTC ACTACTGACC 
CCTCAGACAC TCTTTCCACC ACCACCTCAA CCAAGAGAAA GGGGTGGATC TCTAGTTGAT 
CTACCTTCAC AAGACTTAAG CACCCTTTCC ATGTCCCTTC CTATTCCTGG ACTAGCCGAA 
AAATCCAGGG CTGTGGGAAG AGGACCACAA CCACCAGACT TATCTCATCA CCGAGGATCC 
ACTTCAGGTT ACTCACCACT CCCATCCCCC CCACCCCTTG TGAATCCATT TAGCTCAATA 
CCTGGTCATG TTGTCTTATC GAGACAAACT TCCAAGTTGT CCATAACTCA GACTGGGTCC 
TCAGGTGGAT CACCAGCCCA TGGGTCAAGT GACAGGTCCA CCCCTGGGAC ACCAAGCTTG 
CACCCCAAAC TCCCATCAAA CTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed