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LOC100495521 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol LOC100495521
Entrez Gene ID 100495521
Full Name potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_018092393.2 XP_017947882.2 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
XM_018092393.1 XP_017947882.1 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

Pathways from BioSystems


Homologies


HCN4 XP_002804905.1
Mus musculus (house mouse) Hcn4 NP_001074661.1
Danio rerio (zebrafish) hcn4 XP_686078.4
Xenopus tropicalis (tropical clawed frog) hcn4 XP_004916024.1
Pan troglodytes (chimpanzee) HCN4 XP_003952750.1
Bos taurus (cattle) HCN4 XP_002690539.1
Gallus gallus (chicken) HCN4 XP_425050.3
Homo sapiens (human) HCN4 NP_005468.1
Danio rerio (zebrafish) hcn4l XP_684730.5
Rattus norvegicus (Norway rat) Hcn4 NP_067690.1

Gene Ontology

The following LOC100495521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100495521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa43344 XM_018092393.1
Latest version!		 Xenopus tropicalis potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (LOC100495521), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $426.30
$609.00
OXa55001 XM_018092393.2
Latest version!		 Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 4 (LOC100495521), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa43344
Clone ID Related Accession (Same CDS sequence) XM_018092393.1
Accession Version XM_018092393.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTGCTGC TCATGGTGGG AAACCTCATT ATCATCCCAG TAGGCATCAC TTTTTTCAAG 
GAGGAGAATA CACCAGCATG GATTGTGTTC AATGTGGTGT CGGACACATT TTTCCTTGTG 
GACCTAGTGC TAAATTTCCG TACAGGAATT GTGTTGGAAG ATAATACTGA GATCATCTTG 
GAGCCTCAAC GTATTAAGAT ACGTTACTTA AAGAGTTGGT TCCTGGTAGA CTTTGTGTCC 
TCCATCCCCG TTGACTATAT TTTCCTGATT GTGGAGACAA GAATCGATTC TGAAGTCTAC 
AAGACTGCAA GGGCCCTGCG CATCGTCCGC TTTGCTAAGG TTCTGAGCCT GCTGCGTCTT 
CTGAGACTCT CCAGGCTCAT TCGATACATT CACCAGTGGG AAGAGATATT TCATATGACC 
TATGATCTGG CAAGTGCTGT AGTGCGAATT GTAAACCTGA TAGGCATGAT GCTCCTGCTT 
TGCCATTGGG ATGGCTGCTT ACAGTTCCTA GTGCCCATGC TGCAAGATTT CCCTGAAGAC 
TGCTGGGTTT CTCTTAACCA CATGCAGAAT GCCTCCTGGG GAAAACAGTA CTCTTATGCC 
CTGTTCAAGG CTATGAGCCA TATGCTCTGC ATCGGGTATG GCCAACAGGC ACCAGTGGGC 
ATGGGGGATG TCTGGCTCAC TATGCTCAGT ATGATTGTTG GTGCTACTTG CTACGCCATG 
TTTATTGGTC ATGCCACAGC CCTGATCCAG TCACTGGACT CTTCAAGGCG CCAGTATCAA 
GAGAAGTACA AACAGGTGGA GCAATATATG TCGTTTCACA AACTCCCTGC TGATTTGCGC 
CAGCGAATCC ATGACTACTA TGAGCATCGC TACCAAGGCA AGATGTTTGA TGAAGAGAGC 
ATTCTGGGGG AACTGAGTGA GCCACTTAGA GAGGAAATTA TTAACTTTAA TTGCCGCAAG 
CTGGTGGCAT CCATGCCGCT TTTTGCCAAT GCCGACCCGA ACTTTGTGAC TTCAATGTTG 
ACAAAACTGA GGTTTGAGGT ATTTCAGCCC GGTGATTACT TAATCCGGGA AGGAACTGTG 
GGTAAAAAAA TGTTCTTCAT CCAGCATGGG GTGGTTAGCA TCCTGAGCAA GGGCAGCAAG 
GAGACCAAGC TTTCAGATGG ATCCTACTTT GGAGAGATTT GCCTCCTGAC CCGTGGCAGG 
CGTACTGCGA GCGTCAGAGC TGACACTTAT TGCCGACTCT ATTCCCTGAG TGTCGACCAC 
TTCAACGAGG TTCTTGAAGA GTACCCCATG ATGAGACGTG CCTTTGAAAG TGTTGCGCTA 
GACCGTTTGG ACAGAATTGG TAAAAAGAAC TCCCTTCTTC TGCACAAAGT ACAGCACGAC 
CTACGCTCTG GGGTGCTCAA CTACCATGAG AATGAAATTA TCCAGCAGAT AGTGCAGCAT 
GACCGGGAGA TGGCTCAAGG ACCCCGCTGC AGTCAGACTG AAGAGGAATC TACTTCATTA 
CCAGCCACAC CAGTTATCTG GACCCCACTG GTACAGGCAC CCCTACGAGC CGCGGCTGCT 
ACCACATCAG TGGCTATACC CCTCACCCAC CAGCGCATAC CAGCCACCAT ATTTCGACCA 
GCCCCCAGTG CACTAGGTGT AACAGCCCGT GGATTACGAC AAGCTCAGAG AACTGCTCTC 
CCTACCCACC TAATAGGCAT TGAGGGAGCA GTGTCTCAGC CATCTTCTTT TCATGTTCAC 
CAGCTGGCAG GGTTCTCAGC AGCTGCTTGT GTGAGTCCTG CTCCTCCGCC ATCTGTGCCA 
CCTCTTCAGA GACATATAGT AAAGCACCTG TCTACATATT CCCCACCAGC TGAGCCTCTG 
CAGTTGGTAA CAGGTGAAGC CAACTCTTCC ATATCCCTTT CACCTGAACT GTCACAATCA 
GACCAGCAGT TTCGACCTCT CTCATCTGGA CAGTGCCAAT ACCCTGTTAG TCATTCATTA 
GATACCCATT TACCAGGAGC GTCAGAGCCA CAATGGGTGC TTCCCCCTCC TCCACATAAA 
GCAGACTCTC CCGGGAAAGC CACTGCAGTA CTCAGTCCTG GACCAGTCCG GACCGCTCCT 
ATAGGTGGTT CTTCACGATC TCTTCTCCTT CCTGAAATTA CTGCACTCTC AAATCTCCCC 
AACAGTTGTA CAGCCCTTCC CCTCAACCGG CTAAGGCAGG ATGGTCGCCC CATCTCTGTG 
TCACAGCCCT CTTTGCCTCA AGCACCAGCA TCTGGTTCCC CAAACTTTCC ACAGCAATTT 
ATTCCTGATT TTTCTTCACT TCCCAGAAGT GAGAAATCAA ATATAGGACA TGTAACGCTG 
CCACGAAAAG GCCCCTCAGT GTTAGGTTTG GGAGTACAGA GTATTTGTGG GGAAGACCTT 
TCCCATACCA AACTCCCTAG TAACCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017947882.1
CDS14..2443
Translation

Target ORF information:

RefSeq Version XM_018092393.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (LOC100495521), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_018092393.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTGCTGC TCATGGTGGG AAACCTCATT ATCATCCCAG TAGGCATCAC TTTTTTCAAG 
GAGGAGAATA CACCAGCATG GATTGTGTTC AATGTGGTGT CGGACACATT TTTCCTTGTG 
GACCTAGTGC TAAATTTCCG TACAGGAATT GTGTTGGAAG ATAATACTGA GATCATCTTG 
GAGCCTCAAC GTATTAAGAT ACGTTACTTA AAGAGTTGGT TCCTGGTAGA CTTTGTGTCC 
TCCATCCCCG TTGACTATAT TTTCCTGATT GTGGAGACAA GAATCGATTC TGAAGTCTAC 
AAGACTGCAA GGGCCCTGCG CATCGTCCGC TTTGCTAAGG TTCTGAGCCT GCTGCGTCTT 
CTGAGACTCT CCAGGCTCAT TCGATACATT CACCAGTGGG AAGAGATATT TCATATGACC 
TATGATCTGG CAAGTGCTGT AGTGCGAATT GTAAACCTGA TAGGCATGAT GCTCCTGCTT 
TGCCATTGGG ATGGCTGCTT ACAGTTCCTA GTGCCCATGC TGCAAGATTT CCCTGAAGAC 
TGCTGGGTTT CTCTTAACCA CATGCAGAAT GCCTCCTGGG GAAAACAGTA CTCTTATGCC 
CTGTTCAAGG CTATGAGCCA TATGCTCTGC ATCGGGTATG GCCAACAGGC ACCAGTGGGC 
ATGGGGGATG TCTGGCTCAC TATGCTCAGT ATGATTGTTG GTGCTACTTG CTACGCCATG 
TTTATTGGTC ATGCCACAGC CCTGATCCAG TCACTGGACT CTTCAAGGCG CCAGTATCAA 
GAGAAGTACA AACAGGTGGA GCAATATATG TCGTTTCACA AACTCCCTGC TGATTTGCGC 
CAGCGAATCC ATGACTACTA TGAGCATCGC TACCAAGGCA AGATGTTTGA TGAAGAGAGC 
ATTCTGGGGG AACTGAGTGA GCCACTTAGA GAGGAAATTA TTAACTTTAA TTGCCGCAAG 
CTGGTGGCAT CCATGCCGCT TTTTGCCAAT GCCGACCCGA ACTTTGTGAC TTCAATGTTG 
ACAAAACTGA GGTTTGAGGT ATTTCAGCCC GGTGATTACT TAATCCGGGA AGGAACTGTG 
GGTAAAAAAA TGTTCTTCAT CCAGCATGGG GTGGTTAGCA TCCTGAGCAA GGGCAGCAAG 
GAGACCAAGC TTTCAGATGG ATCCTACTTT GGAGAGATTT GCCTCCTGAC CCGTGGCAGG 
CGTACTGCGA GCGTCAGAGC TGACACTTAT TGCCGACTCT ATTCCCTGAG TGTCGACCAC 
TTCAACGAGG TTCTTGAAGA GTACCCCATG ATGAGACGTG CCTTTGAAAG TGTTGCGCTA 
GACCGTTTGG ACAGAATTGG TAAAAAGAAC TCCCTTCTTC TGCACAAAGT ACAGCACGAC 
CTACGCTCTG GGGTGCTCAA CTACCATGAG AATGAAATTA TCCAGCAGAT AGTGCAGCAT 
GACCGGGAGA TGGCTCAAGG ACCCCGCTGC AGTCAGACTG AAGAGGAATC TACTTCATTA 
CCAGCCACAC CAGTTATCTG GACCCCACTG GTACAGGCAC CCCTACGAGC CGCGGCTGCT 
ACCACATCAG TGGCTATACC CCTCACCCAC CAGCGCATAC CAGCCACCAT ATTTCGACCA 
GCCCCCAGTG CACTAGGTGT AACAGCCCGT GGATTACGAC AAGCTCAGAG AACTGCTCTC 
CCTACCCACC TAATAGGCAT TGAGGGAGCA GTGTCTCAGC CATCTTCTTT TCATGTTCAC 
CAGCTGGCAG GGTTCTCAGC AGCTGCTTGT GTGAGTCCTG CTCCTCCGCC ATCTGTGCCA 
CCTCTTCAGA GACATATAGT AAAGCACCTG TCTACATATT CCCCACCAGC TGAGCCTCTG 
CAGTTGGTAA CAGGTGAAGC CAACTCTTCC ATATCCCTTT CACCTGAACT GTCACAATCA 
GACCAGCAGT TTCGACCTCT CTCATCTGGA CAGTGCCAAT ACCCTGTTAG TCATTCATTA 
GATACCCATT TACCAGGAGC GTCAGAGCCA CAATGGGTGC TTCCCCCTCC TCCACATAAA 
GCAGACTCTC CCGGGAAAGC CACTGCAGTA CTCAGTCCTG GACCAGTCCG GACCGCTCCT 
ATAGGTGGTT CTTCACGATC TCTTCTCCTT CCTGAAATTA CTGCACTCTC AAATCTCCCC 
AACAGTTGTA CAGCCCTTCC CCTCAACCGG CTAAGGCAGG ATGGTCGCCC CATCTCTGTG 
TCACAGCCCT CTTTGCCTCA AGCACCAGCA TCTGGTTCCC CAAACTTTCC ACAGCAATTT 
ATTCCTGATT TTTCTTCACT TCCCAGAAGT GAGAAATCAA ATATAGGACA TGTAACGCTG 
CCACGAAAAG GCCCCTCAGT GTTAGGTTTG GGAGTACAGA GTATTTGTGG GGAAGACCTT 
TCCCATACCA AACTCCCTAG TAACCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa55001
Clone ID Related Accession (Same CDS sequence) XM_018092393.2
Accession Version XM_018092393.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGACAGGT TGCACCCCTC TATGAGGAAA CGTTTGTACA GCCTCCCCCA GCACATCGGA 
TTCAAAGCCT CTATAATGGA TGAAGCAGAA TGTGAAGAAA GGGACACCTG GAGAAAAAGC 
ATCAAACTCA AGCCATTGCC CTCACCTTCT GCCCTTAGAG TCCTTGACCC CCGCCATGGA 
GCAAAGGAAA CCCATATAGT TATGGAAACT GAGGTGGGCA AAGCCCAAAG AACCAGCAGC 
AATGGAGATT GCAGGAGATT CACTGGCAGC CTTTCTTCTA TCACCAGCTG TGGCCGGCAC 
CTTCATGATG CAGATCCCAG TGAGGGGAGA AGGCTTATTT CTGATGGTGA CATTAGTCCC 
ACTGAAGAGT GCTCCCCCAC CCTAGATACA GCTGTAGGTG AATCAAATAT TCATCTCTCC 
CAAAACACTC AGACATCCTC CCAATGTCAG GGAGCTGAGC AACTCAGCAG TCCTGGGAAG 
CTGGATGGAA GGGAGCAGAC TTTGTCTGGT GGGGAAGATG AGCAGGGAAG CTTTATGCAC 
AGACAGTTTG GGGCACTACT GCAACCCGGG GTGAACAAAT TCTCCTTGAG GATGTTTGGC 
AGTCAGAAGG CAGTAGAAAG AGAACAGGAT CGTGTAAAGT CTGCAGGATT TTGGATTATT 
CACCCTTACA GTGACTTCAG GTTTTATTGG GACCTTACCA TGTTGCTGCT CATGGTGGGA 
AACCTCATTA TCATCCCAGT AGGCATCACT TTTTTCAAGG AGGAGAATAC ACCAGCATGG 
ATTGTGTTCA ATGTGGTGTC GGACACATTT TTCCTTGTGG ACCTAGTGCT AAATTTCCGT 
ACAGGAATTG TGTTGGAAGA TAATACTGAG ATCATCTTGG AGCCTCAACG TATTAAGATA 
CGTTACTTAA AGAGTTGGTT CCTGGTAGAC TTTGTGTCCT CCATCCCCGT TGACTATATT 
TTCCTGATTG TGGAGACAAG AATCGATTCT GAAGTCTACA AGACTGCAAG GGCCCTGCGC 
ATTGTCCGCT TTGCTAAGGT TCTGAGCCTG CTGCGTCTTC TGAGACTCTC CAGGCTCATT 
CGATACATTC ACCAGTGGGA AGAGATATTT CATATGACCT ATGATCTGGC AAGTGCTGTA 
GTGCGAATTG TAAACCTGAT AGGCATGATG CTCCTGCTTT GCCATTGGGA TGGCTGCTTA 
CAGTTCCTAG TGCCCATGCT GCAAGATTTC CCTGAAGACT GCTGGGTTTC TCTTAACCAC 
ATGCAGAATG CCTCCTGGGG AAAACAGTAC TCTTATGCCC TGTTCAAGGC TATGAGCCAT 
ATGCTCTGCA TCGGGTATGG CCAACAGGCA CCAGTGGGCA TGGGGGATGT CTGGCTCACT 
ATGCTCAGTA TGATTGTTGG TGCTACTTGC TACGCCATGT TTATTGGTCA TGCCACAGCC 
CTGATCCAGT CACTGGACTC TTCAAGGCGC CAGTATCAAG AGAAGTACAA ACAGGTGGAG 
CAATATATGT CGTTTCACAA ACTCCCTGCT GATTTGCGCC AGCGAATCCA TGACTACTAT 
GAGCATCGCT ACCAAGGCAA GATGTTTGAT GAAGAGAGCA TTCTGGGGGA ACTGAGTGAG 
CCACTTAGAG AGGAAATTAT TAACTTTAAT TGCCGCAAGC TGGTGGCATC CATGCCGCTT 
TTTGCCAATG CCGACCCGAA CTTTGTGACT TCAATGTTGA CAAAACTGAG GTTTGAGGTA 
TTTCAGCCCG GTGATTACTT AATCCGGGAA GGAACTGTGG GTAAAAAAAT GTTCTTCATC 
CAGCATGGGG TGGTTAGCAT CCTGAGCAAG GGCAGCAAGG AGACCAAGCT TTCAGATGGA 
TCCTACTTTG GAGAGATTTG CCTCCTGACC CGTGGCAGGC GTACTGCGAG CGTCAGAGCT 
GACACTTATT GCCGACTCTA TTCCCTGAGT GTCGACCACT TCAACGAGGT TCTTGAAGAG 
TACCCCATGA TGAGACGTGC CTTTGAAAGT GTTGCGCTAG ACCGTTTGGA CAGAATTGGT 
AAAAAGAACT CCCTTCTTCT GCACAAAGTA CAGCACGACC TACGCTCTGG GGTGCTCAAC 
TACCATGAGA ATGAAATTAT CCAGCAGATA GTGCAGCATG ACCGGGAGAT GGCTCAAGGA 
CCCCGCTGCA GTCAGACTGA AGAGGAATCT ACTTCATTAC CAGCCACACC AGTTATCTGG 
ACCCCACTGG TACAGGCACC CCTACGAGCC GCGGCTGCTA CCACATCAGT GGCTATACCC 
CTCACCCACC AGCGCATACC AGCCACCATA TTTCGACCAG CCCCCAGTGC ACTAGGTGTA 
ACAGCCCGTG GATTACGACA AGCTCAGAGA ACTGCTCTCC CTACCCACCT AATAGGCATT 
GAGGGAGCAG TGTCTCAGCC ATCTTCTTTT CATGTACACC AGCTGGCAGG GTTCTCAGCA 
GCTGCTTGTG TGAGTCCTGC TCCTCCGCCA TCTGTGCCAC CTCTTCAGAG ACATATAGTA 
AAGCACCTGT CTACATATTC CCCACCAGCT GAGCCTCTGC AGTTGGTAAC AGGTGAAGCC 
AACTCTTCCA TATCCCTTTC ACCTGAACTG TCACAATCAG ACCAGCAGTT TCGACCTCTC 
TCATCTGGAC AGTGCCAATA CCCTGTTAGT CATTCATTAG ATACCCATTT ACCAGGAGCG 
TCAGAGCCAC AATGGGTGCT TCCCCCTCCT CCACATAAAG CAGACTCTCC CGGGAAAGCC 
ACTGCAGTAC TCAGTCCTGG ACCAGTCCGG ACCGCTCCTA TAGGTGGTTC TTCACGATCT 
CTTCTCCTTC CTGAAATTAC TGCACTCTCA AATCTCCCCA ACAGTTGTAC AGCCCTTCCC 
CTCAACCGGC TAAGGCAGGA TGGTCGCCCC ATCTCTGTGT CACAGCCCTC TTTGCCTCAA 
GCACCAGCAT CTGGTTCCCC AAACTTTCCA CAGCAATTTA TTCCTGATTT TTCTTCACTT 
CCCAGAAGTG AGAAATCAAA TATAGGACAT GTAACGCTAC CACGAAAAGG CCCCTCAGTG 
TTAGGTTTGG GAGTACAGAG TATTTGTGGG GAAGACCTTT CCCATACCAA ACTCCCTAGT 
AACCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017947882.2
CDS342..3470
Translation

Target ORF information:

RefSeq Version XM_018092393.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 4 (LOC100495521), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_018092393.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGACAGGT TGCACCCCTC TATGAGGAAA CGTTTGTACA GCCTCCCCCA GCACATCGGA 
TTCAAAGCCT CTATAATGGA TGAAGCAGAA TGTGAAGAAA GGGACACCTG GAGAAAAAGC 
ATCAAACTCA AGCCATTGCC CTCACCTTCT GCCCTTAGAG TCCTTGACCC CCGCCATGGA 
GCAAAGGAAA CCCATATAGT TATGGAAACT GAGGTGGGCA AAGCCCAAAG AACCAGCAGC 
AATGGAGATT GCAGGAGATT CACTGGCAGC CTTTCTTCTA TCACCAGCTG TGGCCGGCAC 
CTTCATGATG CAGATCCCAG TGAGGGGAGA AGGCTTATTT CTGATGGTGA CATTAGTCCC 
ACTGAAGAGT GCTCCCCCAC CCTAGATACA GCTGTAGGTG AATCAAATAT TCATCTCTCC 
CAAAACACTC AGACATCCTC CCAATGTCAG GGAGCTGAGC AACTCAGCAG TCCTGGGAAG 
CTGGATGGAA GGGAGCAGAC TTTGTCTGGT GGGGAAGATG AGCAGGGAAG CTTTATGCAC 
AGACAGTTTG GGGCACTACT GCAACCCGGG GTGAACAAAT TCTCCTTGAG GATGTTTGGC 
AGTCAGAAGG CAGTAGAAAG AGAACAGGAT CGTGTAAAGT CTGCAGGATT TTGGATTATT 
CACCCTTACA GTGACTTCAG GTTTTATTGG GACCTTACCA TGTTGCTGCT CATGGTGGGA 
AACCTCATTA TCATCCCAGT AGGCATCACT TTTTTCAAGG AGGAGAATAC ACCAGCATGG 
ATTGTGTTCA ATGTGGTGTC GGACACATTT TTCCTTGTGG ACCTAGTGCT AAATTTCCGT 
ACAGGAATTG TGTTGGAAGA TAATACTGAG ATCATCTTGG AGCCTCAACG TATTAAGATA 
CGTTACTTAA AGAGTTGGTT CCTGGTAGAC TTTGTGTCCT CCATCCCCGT TGACTATATT 
TTCCTGATTG TGGAGACAAG AATCGATTCT GAAGTCTACA AGACTGCAAG GGCCCTGCGC 
ATTGTCCGCT TTGCTAAGGT TCTGAGCCTG CTGCGTCTTC TGAGACTCTC CAGGCTCATT 
CGATACATTC ACCAGTGGGA AGAGATATTT CATATGACCT ATGATCTGGC AAGTGCTGTA 
GTGCGAATTG TAAACCTGAT AGGCATGATG CTCCTGCTTT GCCATTGGGA TGGCTGCTTA 
CAGTTCCTAG TGCCCATGCT GCAAGATTTC CCTGAAGACT GCTGGGTTTC TCTTAACCAC 
ATGCAGAATG CCTCCTGGGG AAAACAGTAC TCTTATGCCC TGTTCAAGGC TATGAGCCAT 
ATGCTCTGCA TCGGGTATGG CCAACAGGCA CCAGTGGGCA TGGGGGATGT CTGGCTCACT 
ATGCTCAGTA TGATTGTTGG TGCTACTTGC TACGCCATGT TTATTGGTCA TGCCACAGCC 
CTGATCCAGT CACTGGACTC TTCAAGGCGC CAGTATCAAG AGAAGTACAA ACAGGTGGAG 
CAATATATGT CGTTTCACAA ACTCCCTGCT GATTTGCGCC AGCGAATCCA TGACTACTAT 
GAGCATCGCT ACCAAGGCAA GATGTTTGAT GAAGAGAGCA TTCTGGGGGA ACTGAGTGAG 
CCACTTAGAG AGGAAATTAT TAACTTTAAT TGCCGCAAGC TGGTGGCATC CATGCCGCTT 
TTTGCCAATG CCGACCCGAA CTTTGTGACT TCAATGTTGA CAAAACTGAG GTTTGAGGTA 
TTTCAGCCCG GTGATTACTT AATCCGGGAA GGAACTGTGG GTAAAAAAAT GTTCTTCATC 
CAGCATGGGG TGGTTAGCAT CCTGAGCAAG GGCAGCAAGG AGACCAAGCT TTCAGATGGA 
TCCTACTTTG GAGAGATTTG CCTCCTGACC CGTGGCAGGC GTACTGCGAG CGTCAGAGCT 
GACACTTATT GCCGACTCTA TTCCCTGAGT GTCGACCACT TCAACGAGGT TCTTGAAGAG 
TACCCCATGA TGAGACGTGC CTTTGAAAGT GTTGCGCTAG ACCGTTTGGA CAGAATTGGT 
AAAAAGAACT CCCTTCTTCT GCACAAAGTA CAGCACGACC TACGCTCTGG GGTGCTCAAC 
TACCATGAGA ATGAAATTAT CCAGCAGATA GTGCAGCATG ACCGGGAGAT GGCTCAAGGA 
CCCCGCTGCA GTCAGACTGA AGAGGAATCT ACTTCATTAC CAGCCACACC AGTTATCTGG 
ACCCCACTGG TACAGGCACC CCTACGAGCC GCGGCTGCTA CCACATCAGT GGCTATACCC 
CTCACCCACC AGCGCATACC AGCCACCATA TTTCGACCAG CCCCCAGTGC ACTAGGTGTA 
ACAGCCCGTG GATTACGACA AGCTCAGAGA ACTGCTCTCC CTACCCACCT AATAGGCATT 
GAGGGAGCAG TGTCTCAGCC ATCTTCTTTT CATGTACACC AGCTGGCAGG GTTCTCAGCA 
GCTGCTTGTG TGAGTCCTGC TCCTCCGCCA TCTGTGCCAC CTCTTCAGAG ACATATAGTA 
AAGCACCTGT CTACATATTC CCCACCAGCT GAGCCTCTGC AGTTGGTAAC AGGTGAAGCC 
AACTCTTCCA TATCCCTTTC ACCTGAACTG TCACAATCAG ACCAGCAGTT TCGACCTCTC 
TCATCTGGAC AGTGCCAATA CCCTGTTAGT CATTCATTAG ATACCCATTT ACCAGGAGCG 
TCAGAGCCAC AATGGGTGCT TCCCCCTCCT CCACATAAAG CAGACTCTCC CGGGAAAGCC 
ACTGCAGTAC TCAGTCCTGG ACCAGTCCGG ACCGCTCCTA TAGGTGGTTC TTCACGATCT 
CTTCTCCTTC CTGAAATTAC TGCACTCTCA AATCTCCCCA ACAGTTGTAC AGCCCTTCCC 
CTCAACCGGC TAAGGCAGGA TGGTCGCCCC ATCTCTGTGT CACAGCCCTC TTTGCCTCAA 
GCACCAGCAT CTGGTTCCCC AAACTTTCCA CAGCAATTTA TTCCTGATTT TTCTTCACTT 
CCCAGAAGTG AGAAATCAAA TATAGGACAT GTAACGCTAC CACGAAAAGG CCCCTCAGTG 
TTAGGTTTGG GAGTACAGAG TATTTGTGGG GAAGACCTTT CCCATACCAA ACTCCCTAGT 
AACCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed