ORF » Species Summary » Danio rerio » ntn4 cDNA ORF clone

ntn4 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following ntn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ntn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa04710 NM_001037686.1
Latest version!		 Danio rerio netrin 4 (ntn4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa43511 XM_009303070.3
Latest version!		 Danio rerio netrin 4 (ntn4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa43511 XM_009303070.2 Danio rerio netrin 4 (ntn4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ntn4 ( NM_001037686.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032775.1 Danio rerio netrin 4 (ntn4), mRNA.
    Price & Availability↑
    CloneID ODa04710
    Clone ID Related Accession (Same CDS sequence) NM_001037686.1
    Accession Version NM_001037686.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-30
    Organism Danio rerio(zebrafish)
    Product netrin-4 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY928519.1. On Dec 10, 2005 this sequence version replaced XM_684845.1. ##Evidence-Data-START## Transcript exon combination :: AY928519.1 [ECO:0000332] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAGCTGA CGGGGAGATG TGTGTTGTTG GCGGCGCTGG TGATGAGCGT TCCTCACCAC 
    GCGTGTTCAG GATGTGTGAA CCGAGTGTGT AACCCACGCA TGGGAAATCT GGCAGTCGGA 
    CGTCCCATAC AGACAATCTC TCAGTGCGGC TCCACCTCCC CAGAACAATT CTGTATGTAC 
    AGACCGGACA GTTGTGTGCT AGATTGTGAG ATATGTGATG CTTCAGTATC TCAGCATGCA 
    CACTCCCCTT CCTCCATGAC TGACTCTCCT TTCAAGCAGC CACTGACCTG GTGGCAGTCG 
    GCACCGGGGA CTGCCATAGA GACTCTGCAG CTGGATCTGG AGGTGGAGTT TTACTTCACC 
    CACCTCATTA TCATTTTCAT GTCGCCACGC CCTGCTGCCA TGGCAATCGA TCGCTCTCGG 
    GACTTTGGCC AGACATGGAG CTTGCTGATG CTGTTTTCCC ACAACTGCAG TGAAGTGTCC 
    AGCTTGGAGG ACGGACAACG CTGCACAGCG AAATACTCCT CCGCCGAGCC ATGCACAAAT 
    GGGGAGGTGC CACACTCCTG CCCCTGTCCG ATTGCTGATG CCAATGCAGC AACTCCCCGA 
    TATGCCATCT CTGACCTCAT TTTAAAAGGA GGCTGCCTGT GCCATGGACA TGCAGACCAG 
    TGTGTGCCCA CTGGAGGCAA ACACAACTCA AAAACATTTG GAAAACACGT GGTGCACGGT 
    AAATGCTCAT GCCGGCATCA CACGGCTGGA GATCACTGTG AACGCTGCCA TCGCCTTTAC 
    AATGACAGAC CATGGAGAGC AGCTGACGGC TTAACAGGAG AAGCAAATCA GTGTGTTGAG 
    TGTAAGTGTA ATGGCCATGC GGAGAGCTGT CACTTTGATG AAAGCGTATG GCTGCGGTCG 
    GGTCAGCAGC ACGGTGGCGT TTGTGACTGT CTTCACAACA CCACCGGCCA ACACTGCCAA 
    CACTGTCAGA GCGGCTTCTA CAGAGATCCG AAGAAACCCT CCACCGCCCC AGATTCCTGC 
    ACACCTTGTA TGTGTAACCA TGTCGGTTCC TCATATTGTA AACCTGATAA CGGAGAGTGT 
    GTCTGTAAGC CAGGGGTGGC TGGGAGGCAC TGTGAGCAGT GCATGCTGGG ATACTGGGGT 
    TTGGATGAGT ACGGTTGCAA GCCATGTCAG TGTGCTGGGG ATTGTGACCC TTACACCGGA 
    GACTGTATGA TTAGGTCTGA GTCAGATGAC AACAGCACAG CAGAAAATCA TCTGTTTCGG 
    ACAGAAGAGC TGTTCTCCGC CCTCAGTCAT CCAGACAAAT GCATTTGCAA ACAGCTAGCT 
    CTCGGGAACA GCAAAGTCTT CTGCAATGCC AAATATGCTT ATGCAGTGAA AGTGCGAGTG 
    TTGGGTGCTC ATGATAAAGG CTCTCATGCA GAGGTGGATG TGAAGATCCT AAAGGTATTG 
    TGGGACAACA CTCGCCTCAG ACTCTCTCGA GGAAGCCGGA CACTTTATCC CGAGTCCTGG 
    ATGTCTCGAG GCTGCACCTG CCCCGTGCTC TTTCCAGGTG GATTGGAGTA TCTTGTGGTG 
    GGACACGAGG ATGTGAGAAA AGGTCGCCCT GTTGTTAACA TGAAGAGCAT AGTGAAGCCA 
    TGGAGAGCCA GCTTGAGTCG GAAAGTCCAT CAGCTGCTGA AAACCAAATG TAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001032775.1
    CDS379..2055
    Translation

    Target ORF information:

    RefSeq Version NM_001037686.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin 4 (ntn4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001037686.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAGCTGA CGGGGAGATG TGTGTTGTTG GCGGCGCTGG TGATGAGCGT TCCTCACCAC 
    GCGTGTTCAG GATGTGTGAA CCGAGTGTGT AACCCACGCA TGGGAAATCT GGCAGTCGGA 
    CGTCCCATAC AGACAATCTC TCAGTGCGGC TCCACCTCCC CAGAACAATT CTGTATGTAC 
    AGACCGGACA GTTGTGTGCT AGATTGTGAG ATATGTGATG CTTCAGTATC TCAGCATGCA 
    CACTCCCCTT CCTCCATGAC TGACTCTCCT TTCAAGCAGC CACTGACCTG GTGGCAGTCG 
    GCACCGGGGA CTGCCATAGA GACTCTGCAG CTGGATCTGG AGGTGGAGTT TTACTTCACC 
    CACCTCATTA TCATTTTCAT GTCGCCACGC CCTGCTGCCA TGGCAATCGA TCGCTCTCGG 
    GACTTTGGCC AGACATGGAG CTTGCTGATG CTGTTTTCCC ACAACTGCAG TGAAGTGTCC 
    AGCTTGGAGG ACGGACAACG CTGCACAGCG AAATACTCCT CCGCCGAGCC ATGCACAAAT 
    GGGGAGGTGC CACACTCCTG CCCCTGTCCG ATTGCTGATG CCAATGCAGC AACTCCCCGA 
    TATGCCATCT CTGACCTCAT TTTAAAAGGA GGCTGCCTGT GCCATGGACA TGCAGACCAG 
    TGTGTGCCCA CTGGAGGCAA ACACAACTCA AAAACATTTG GAAAACACGT GGTGCACGGT 
    AAATGCTCAT GCCGGCATCA CACGGCTGGA GATCACTGTG AACGCTGCCA TCGCCTTTAC 
    AATGACAGAC CATGGAGAGC AGCTGACGGC TTAACAGGAG AAGCAAATCA GTGTGTTGAG 
    TGTAAGTGTA ATGGCCATGC GGAGAGCTGT CACTTTGATG AAAGCGTATG GCTGCGGTCG 
    GGTCAGCAGC ACGGTGGCGT TTGTGACTGT CTTCACAACA CCACCGGCCA ACACTGCCAA 
    CACTGTCAGA GCGGCTTCTA CAGAGATCCG AAGAAACCCT CCACCGCCCC AGATTCCTGC 
    ACACCTTGTA TGTGTAACCA TGTCGGTTCC TCATATTGTA AACCTGATAA CGGAGAGTGT 
    GTCTGTAAGC CAGGGGTGGC TGGGAGGCAC TGTGAGCAGT GCATGCTGGG ATACTGGGGT 
    TTGGATGAGT ACGGTTGCAA GCCATGTCAG TGTGCTGGGG ATTGTGACCC TTACACCGGA 
    GACTGTATGA TTAGGTCTGA GTCAGATGAC AACAGCACAG CAGAAAATCA TCTGTTTCGG 
    ACAGAAGAGC TGTTCTCCGC CCTCAGTCAT CCAGACAAAT GCATTTGCAA ACAGCTAGCT 
    CTCGGGAACA GCAAAGTCTT CTGCAATGCC AAATATGCTT ATGCAGTGAA AGTGCGAGTG 
    TTGGGTGCTC ATGATAAAGG CTCTCATGCA GAGGTGGATG TGAAGATCCT AAAGGTATTG 
    TGGGACAACA CTCGCCTCAG ACTCTCTCGA GGAAGCCGGA CACTTTATCC CGAGTCCTGG 
    ATGTCTCGAG GCTGCACCTG CCCCGTGCTC TTTCCAGGTG GATTGGAGTA TCTTGTGGTG 
    GGACACGAGG ATGTGAGAAA AGGTCGCCCT GTTGTTAACA TGAAGAGCAT AGTGAAGCCA 
    TGGAGAGCCA GCTTGAGTCG GAAAGTCCAT CAGCTGCTGA AAACCAAATG TAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ntn4 ( XM_009303070.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301345.1 Danio rerio netrin 4 (ntn4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa43511
    Clone ID Related Accession (Same CDS sequence) XM_009303070.2 , XM_009303070.3
    Accession Version XM_009303070.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product netrin-4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303070.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAGTCGT GCTCCTACAT GCAGTCTGAA GGGCTCCGGA GCGGAGTCGG GTTCCCGTGT 
    TTATTGTGGA TTAAACCACT TATGCTGCTT GATACGCGTA ATTTGTGGAT TTGTGTGTGT 
    TTTTCTTCTC CTGGATTGAT GAGCACTATG GAGCTGACGG GGAGATGTGT GTTGTTGGCG 
    GCGCTGGTGA TGAGCGTTCC TCACCACGCG TGTTCAGGAT GTGTGAACCG AGTGTGTAAT 
    CCACGCATGG GAAATCTGGC AGTCGGACGT CCCATACAGA CAGTCTCTCA GTGTGGCTCC 
    ACCTCCCCAG AACAATTCTG TATGTACAGA CCGGACAGTT GTGTGCTAGA TTGTGAGATA 
    TGTGATGCTT CAGTATCTCA GCATGCACAC TCCCCTTCCT CCATGACTGA CTCTCCTTTC 
    AAGCAGCCAC TGACCTGGTG GCAGTCGGCA CCGGGGACTG CCATAGAGAC TCTGCAGCTG 
    GATCTGGAGG TGGAGTTTTA CTTCACCCAC CTCATTATCA TTTTCATGTC GCCACGCCCT 
    GCTGCCATGG CAATCGATCG CTCTCGGGAC TTTGGCCAGA CATGGAGCTT GCTGATGCTG 
    TTTTCCCACA ACTGCAGTGA AGTGTCCAGC TTGGAGGACG GACAACGCTG CACAGCGAAA 
    TACTCCTCCG CCGAGCCATG CACAAATGGG GAGGTGATTT ACCGTGCACT GTCACCTTAT 
    GAGAGGCTGG ATCCATACAG CACTGATGCC CGCTCACTGC TGGGCGTCAC TAATGTCAGG 
    ATTCGTCTGC TTCAGCCACA CTCCTGCCCC TGTCCGATTG CTGATGCCAA TGCAGCAACT 
    CCCCGATATG CCATCTCTGA CCTCATTTTA AAAGGAGGCT GCCTGTGCCA TGGACATGCA 
    GACCAGTGTG TGCCCACTGG AGGCAAACAC AACTCAAAAA CATTTGGAAA ACACGTGGTG 
    CACGGTAAAT GCTTATGCCG GCATCACACG GCTGGAGATC ACTGTGAACG CTGCCATCGC 
    CTTTACAATG ACAGACCATG GAGAGCAGCT GACGGCTTAA CAGGAGAAGC AAATCAGTGT 
    GTTGAGTGTA AGTGTAATGG CCATGCGGAG AGCTGTCACT TTGATGAAAG CGTATGGCTG 
    CGGTCGGGTC AGCAGCACGG TGGCGTTTGT GACTGTCTTC ACAACACCAC CGGCCAACAC 
    TGCCAACACT GTCAGAGCGG CTTCTACAGA GATCCGGAGA AACCCTCCAC CGCCCCAGAT 
    TCCTGCACAC CTTGTATGTG TAACCATGTC GGTTCCTCAT ATTGTAAACC TGATAACGGA 
    GAGTGTGTCT GTAAGCCAGG GGTGGCTGGG AGGCACTGTG AGCAGTGCAT GCTGGGATAC 
    TGGGGTTTGG ATGAGTACGG TTGCAAGCCA TGTCAGTGTG CTGGGGATTG TGACCCTTAC 
    ACCGGAGACT GTATGATTAG GTCTGAGTCA GATGACAACA GCACAGCAGA AAATCATCTG 
    TTTCGGACAG AAGAGCTGTT CTCCGCCCTC AGTCATCCAG ACAAATGCAT TTGCAAACAG 
    CAAGCTCTCG GGAACAGCAA AGTCTTCTGC AATGCCAAAT ATGCTTATGC AGTGAAAGTG 
    CGAGTGTTGG GCGCTCATGA TAAAGGCTCT CATGCAGAGG TGGATGTGAA GATCCTAAAG 
    GTATTGTGGG ACAACACTCG CCTCAGACTC TCTCGAGGAA GCCGGACACT TTATCCCGAG 
    TCCTGGATGT CTCGAGGCTG CACCTGCCCC GTGCTCTTTC CAGGATTGGA GTATCTTGTG 
    GTGGGACACG AGGATGTGAG AAAAGGTCGC CCTGTTGTTA ACATGAAGAG CATAGTGAAG 
    CCATGGAGAG CCAGCTTGAG TCGGAAAGTC CATCAGCTGC TGAAAACCAA ATGTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301345.1
    CDS1001..2920
    Translation

    Target ORF information:

    RefSeq Version XM_009303070.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio netrin 4 (ntn4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009303070.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAGTCGT GCTCCTACAT GCAGTCTGAA GGGCTCCGGA GCGGAGTCGG GTTCCCGTGT 
    TTATTGTGGA TTAAACCACT TATGCTGCTT GATACGCGTA ATTTGTGGAT TTGTGTGTGT 
    TTTTCTTCTC CTGGATTGAT GAGCACTATG GAGCTGACGG GGAGATGTGT GTTGTTGGCG 
    GCGCTGGTGA TGAGCGTTCC TCACCACGCG TGTTCAGGAT GTGTGAACCG AGTGTGTAAT 
    CCACGCATGG GAAATCTGGC AGTCGGACGT CCCATACAGA CAGTCTCTCA GTGTGGCTCC 
    ACCTCCCCAG AACAATTCTG TATGTACAGA CCGGACAGTT GTGTGCTAGA TTGTGAGATA 
    TGTGATGCTT CAGTATCTCA GCATGCACAC TCCCCTTCCT CCATGACTGA CTCTCCTTTC 
    AAGCAGCCAC TGACCTGGTG GCAGTCGGCA CCGGGGACTG CCATAGAGAC TCTGCAGCTG 
    GATCTGGAGG TGGAGTTTTA CTTCACCCAC CTCATTATCA TTTTCATGTC GCCACGCCCT 
    GCTGCCATGG CAATCGATCG CTCTCGGGAC TTTGGCCAGA CATGGAGCTT GCTGATGCTG 
    TTTTCCCACA ACTGCAGTGA AGTGTCCAGC TTGGAGGACG GACAACGCTG CACAGCGAAA 
    TACTCCTCCG CCGAGCCATG CACAAATGGG GAGGTGATTT ACCGTGCACT GTCACCTTAT 
    GAGAGGCTGG ATCCATACAG CACTGATGCC CGCTCACTGC TGGGCGTCAC TAATGTCAGG 
    ATTCGTCTGC TTCAGCCACA CTCCTGCCCC TGTCCGATTG CTGATGCCAA TGCAGCAACT 
    CCCCGATATG CCATCTCTGA CCTCATTTTA AAAGGAGGCT GCCTGTGCCA TGGACATGCA 
    GACCAGTGTG TGCCCACTGG AGGCAAACAC AACTCAAAAA CATTTGGAAA ACACGTGGTG 
    CACGGTAAAT GCTTATGCCG GCATCACACG GCTGGAGATC ACTGTGAACG CTGCCATCGC 
    CTTTACAATG ACAGACCATG GAGAGCAGCT GACGGCTTAA CAGGAGAAGC AAATCAGTGT 
    GTTGAGTGTA AGTGTAATGG CCATGCGGAG AGCTGTCACT TTGATGAAAG CGTATGGCTG 
    CGGTCGGGTC AGCAGCACGG TGGCGTTTGT GACTGTCTTC ACAACACCAC CGGCCAACAC 
    TGCCAACACT GTCAGAGCGG CTTCTACAGA GATCCGGAGA AACCCTCCAC CGCCCCAGAT 
    TCCTGCACAC CTTGTATGTG TAACCATGTC GGTTCCTCAT ATTGTAAACC TGATAACGGA 
    GAGTGTGTCT GTAAGCCAGG GGTGGCTGGG AGGCACTGTG AGCAGTGCAT GCTGGGATAC 
    TGGGGTTTGG ATGAGTACGG TTGCAAGCCA TGTCAGTGTG CTGGGGATTG TGACCCTTAC 
    ACCGGAGACT GTATGATTAG GTCTGAGTCA GATGACAACA GCACAGCAGA AAATCATCTG 
    TTTCGGACAG AAGAGCTGTT CTCCGCCCTC AGTCATCCAG ACAAATGCAT TTGCAAACAG 
    CAAGCTCTCG GGAACAGCAA AGTCTTCTGC AATGCCAAAT ATGCTTATGC AGTGAAAGTG 
    CGAGTGTTGG GCGCTCATGA TAAAGGCTCT CATGCAGAGG TGGATGTGAA GATCCTAAAG 
    GTATTGTGGG ACAACACTCG CCTCAGACTC TCTCGAGGAA GCCGGACACT TTATCCCGAG 
    TCCTGGATGT CTCGAGGCTG CACCTGCCCC GTGCTCTTTC CAGGATTGGA GTATCTTGTG 
    GTGGGACACG AGGATGTGAG AAAAGGTCGC CCTGTTGTTA ACATGAAGAG CATAGTGAAG 
    CCATGGAGAG CCAGCTTGAG TCGGAAAGTC CATCAGCTGC TGAAAACCAA ATGTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ntn4 ( XM_009303070.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301345.1 Danio rerio netrin 4 (ntn4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa43511
    Clone ID Related Accession (Same CDS sequence) XM_009303070.2 , XM_009303070.3
    Accession Version XM_009303070.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product netrin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) and transcript sequence (GFIL01028825.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303070.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 446 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAGTCGT GCTCCTACAT GCAGTCTGAA GGGCTCCGGA GCGGAGTCGG GTTCCCGTGT 
    TTATTGTGGA TTAAACCACT TATGCTGCTT GATACGCGTA ATTTGTGGAT TTGTGTGTGT 
    TTTTCTTCTC CTGGATTGAT GAGCACTATG GAGCTGACGG GGAGATGTGT GTTGTTGGCG 
    GCGCTGGTGA TGAGCGTTCC TCACCACGCG TGTTCAGGAT GTGTGAACCG AGTGTGTAAT 
    CCACGCATGG GAAATCTGGC AGTCGGACGT CCCATACAGA CAGTCTCTCA GTGTGGCTCC 
    ACCTCCCCAG AACAATTCTG TATGTACAGA CCGGACAGTT GTGTGCTAGA TTGTGAGATA 
    TGTGATGCTT CAGTATCTCA GCATGCACAC TCCCCTTCCT CCATGACTGA CTCTCCTTTC 
    AAGCAGCCAC TGACCTGGTG GCAGTCGGCA CCGGGGACTG CCATAGAGAC TCTGCAGCTG 
    GATCTGGAGG TGGAGTTTTA CTTCACCCAC CTCATTATCA TTTTCATGTC GCCACGCCCT 
    GCTGCCATGG CAATCGATCG CTCTCGGGAC TTTGGCCAGA CATGGAGCTT GCTGATGCTG 
    TTTTCCCACA ACTGCAGTGA AGTGTCCAGC TTGGAGGACG GACAACGCTG CACAGCGAAA 
    TACTCCTCCG CCGAGCCATG CACAAATGGG GAGGTGATTT ACCGTGCACT GTCACCTTAT 
    GAGAGGCTGG ATCCATACAG CACTGATGCC CGCTCACTGC TGGGCGTCAC TAATGTCAGG 
    ATTCGTCTGC TTCAGCCACA CTCCTGCCCC TGTCCGATTG CTGATGCCAA TGCAGCAACT 
    CCCCGATATG CCATCTCTGA CCTCATTTTA AAAGGAGGCT GCCTGTGCCA TGGACATGCA 
    GACCAGTGTG TGCCCACTGG AGGCAAACAC AACTCAAAAA CATTTGGAAA ACACGTGGTG 
    CACGGTAAAT GCTTATGCCG GCATCACACG GCTGGAGATC ACTGTGAACG CTGCCATCGC 
    CTTTACAATG ACAGACCATG GAGAGCAGCT GACGGCTTAA CAGGAGAAGC AAATCAGTGT 
    GTTGAGTGTA AGTGTAATGG CCATGCGGAG AGCTGTCACT TTGATGAAAG CGTATGGCTG 
    CGGTCGGGTC AGCAGCACGG TGGCGTTTGT GACTGTCTTC ACAACACCAC CGGCCAACAC 
    TGCCAACACT GTCAGAGCGG CTTCTACAGA GATCCGGAGA AACCCTCCAC CGCCCCAGAT 
    TCCTGCACAC CTTGTATGTG TAACCATGTC GGTTCCTCAT ATTGTAAACC TGATAACGGA 
    GAGTGTGTCT GTAAGCCAGG GGTGGCTGGG AGGCACTGTG AGCAGTGCAT GCTGGGATAC 
    TGGGGTTTGG ATGAGTACGG TTGCAAGCCA TGTCAGTGTG CTGGGGATTG TGACCCTTAC 
    ACCGGAGACT GTATGATTAG GTCTGAGTCA GATGACAACA GCACAGCAGA AAATCATCTG 
    TTTCGGACAG AAGAGCTGTT CTCCGCCCTC AGTCATCCAG ACAAATGCAT TTGCAAACAG 
    CAAGCTCTCG GGAACAGCAA AGTCTTCTGC AATGCCAAAT ATGCTTATGC AGTGAAAGTG 
    CGAGTGTTGG GCGCTCATGA TAAAGGCTCT CATGCAGAGG TGGATGTGAA GATCCTAAAG 
    GTATTGTGGG ACAACACTCG CCTCAGACTC TCTCGAGGAA GCCGGACACT TTATCCCGAG 
    TCCTGGATGT CTCGAGGCTG CACCTGCCCC GTGCTCTTTC CAGGATTGGA GTATCTTGTG 
    GTGGGACACG AGGATGTGAG AAAAGGTCGC CCTGTTGTTA ACATGAAGAG CATAGTGAAG 
    CCATGGAGAG CCAGCTTGAG TCGGAAAGTC CATCAGCTGC TGAAAACCAA ATGTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301345.1
    CDS2365..4284
    Translation

    Target ORF information:

    RefSeq Version XM_009303070.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin 4 (ntn4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009303070.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAGTCGT GCTCCTACAT GCAGTCTGAA GGGCTCCGGA GCGGAGTCGG GTTCCCGTGT 
    TTATTGTGGA TTAAACCACT TATGCTGCTT GATACGCGTA ATTTGTGGAT TTGTGTGTGT 
    TTTTCTTCTC CTGGATTGAT GAGCACTATG GAGCTGACGG GGAGATGTGT GTTGTTGGCG 
    GCGCTGGTGA TGAGCGTTCC TCACCACGCG TGTTCAGGAT GTGTGAACCG AGTGTGTAAT 
    CCACGCATGG GAAATCTGGC AGTCGGACGT CCCATACAGA CAGTCTCTCA GTGTGGCTCC 
    ACCTCCCCAG AACAATTCTG TATGTACAGA CCGGACAGTT GTGTGCTAGA TTGTGAGATA 
    TGTGATGCTT CAGTATCTCA GCATGCACAC TCCCCTTCCT CCATGACTGA CTCTCCTTTC 
    AAGCAGCCAC TGACCTGGTG GCAGTCGGCA CCGGGGACTG CCATAGAGAC TCTGCAGCTG 
    GATCTGGAGG TGGAGTTTTA CTTCACCCAC CTCATTATCA TTTTCATGTC GCCACGCCCT 
    GCTGCCATGG CAATCGATCG CTCTCGGGAC TTTGGCCAGA CATGGAGCTT GCTGATGCTG 
    TTTTCCCACA ACTGCAGTGA AGTGTCCAGC TTGGAGGACG GACAACGCTG CACAGCGAAA 
    TACTCCTCCG CCGAGCCATG CACAAATGGG GAGGTGATTT ACCGTGCACT GTCACCTTAT 
    GAGAGGCTGG ATCCATACAG CACTGATGCC CGCTCACTGC TGGGCGTCAC TAATGTCAGG 
    ATTCGTCTGC TTCAGCCACA CTCCTGCCCC TGTCCGATTG CTGATGCCAA TGCAGCAACT 
    CCCCGATATG CCATCTCTGA CCTCATTTTA AAAGGAGGCT GCCTGTGCCA TGGACATGCA 
    GACCAGTGTG TGCCCACTGG AGGCAAACAC AACTCAAAAA CATTTGGAAA ACACGTGGTG 
    CACGGTAAAT GCTTATGCCG GCATCACACG GCTGGAGATC ACTGTGAACG CTGCCATCGC 
    CTTTACAATG ACAGACCATG GAGAGCAGCT GACGGCTTAA CAGGAGAAGC AAATCAGTGT 
    GTTGAGTGTA AGTGTAATGG CCATGCGGAG AGCTGTCACT TTGATGAAAG CGTATGGCTG 
    CGGTCGGGTC AGCAGCACGG TGGCGTTTGT GACTGTCTTC ACAACACCAC CGGCCAACAC 
    TGCCAACACT GTCAGAGCGG CTTCTACAGA GATCCGGAGA AACCCTCCAC CGCCCCAGAT 
    TCCTGCACAC CTTGTATGTG TAACCATGTC GGTTCCTCAT ATTGTAAACC TGATAACGGA 
    GAGTGTGTCT GTAAGCCAGG GGTGGCTGGG AGGCACTGTG AGCAGTGCAT GCTGGGATAC 
    TGGGGTTTGG ATGAGTACGG TTGCAAGCCA TGTCAGTGTG CTGGGGATTG TGACCCTTAC 
    ACCGGAGACT GTATGATTAG GTCTGAGTCA GATGACAACA GCACAGCAGA AAATCATCTG 
    TTTCGGACAG AAGAGCTGTT CTCCGCCCTC AGTCATCCAG ACAAATGCAT TTGCAAACAG 
    CAAGCTCTCG GGAACAGCAA AGTCTTCTGC AATGCCAAAT ATGCTTATGC AGTGAAAGTG 
    CGAGTGTTGG GCGCTCATGA TAAAGGCTCT CATGCAGAGG TGGATGTGAA GATCCTAAAG 
    GTATTGTGGG ACAACACTCG CCTCAGACTC TCTCGAGGAA GCCGGACACT TTATCCCGAG 
    TCCTGGATGT CTCGAGGCTG CACCTGCCCC GTGCTCTTTC CAGGATTGGA GTATCTTGTG 
    GTGGGACACG AGGATGTGAG AAAAGGTCGC CCTGTTGTTA ACATGAAGAG CATAGTGAAG 
    CCATGGAGAG CCAGCTTGAG TCGGAAAGTC CATCAGCTGC TGAAAACCAA ATGTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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