Ntn4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Ntn4
Entrez Gene ID	57764
Full Name	netrin 4
General protein information	Preferred Names netrin 4 Names netrin-4 beta-netrin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C2

mRNA and Protein(s)

mRNA Protein Name

NM_021320.3 NP_067295.2 netrin-4 precursor
Pathways from BioSystems

mRNA	Protein	Name
NM_021320.3	NP_067295.2	netrin-4 precursor

Homologies

Bos taurus (cattle)	NTN4	NP_001095673.1
Mus musculus (house mouse)	Ntn4	NP_067295.2
Danio rerio (zebrafish)	ntn4	NP_001032775.1
Homo sapiens (human)	NTN4	NP_067052.2
Gallus gallus (chicken)	NTN4	XP_004937705.1
Rattus norvegicus (Norway rat)	Ntn4	NP_001100250.1
Pan troglodytes (chimpanzee)	NTN4	XP_003313918.1
	NTN4	XP_001107505.1
Canis lupus familiaris (dog)	NTN4	XP_532655.2

Gene Ontology
Bibliography

Related articles in PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Memic F, Knoflach V, Morarach K, Sadler R, Laranjeira C, Hjerling-Leffler J, Sundstr?m E, Pachnis V, Marklund U
Gastroenterology(2017 Oct)

Netrin-4 Promotes Differentiation and Migration of Osteoblasts.
Enoki Y, Sato T, Kokabu S, Hayashi N, Iwata T, Yamato M, Usui M, Matsumoto M, Tomoda T, Ariyoshi W, Nishihara T, Yoda T
In vivo (Athens, Greece)31(5)793-799(2017 Sep-Oct)

Netrin-4 Mediates Corneal Hemangiogenesis but Not Lymphangiogenesis in the Mouse-Model of Suture-Induced Neovascularization.
Maier AB, Klein S, Kociok N, Riechardt AI, Gundlach E, Reichhart N, Strau? O, Joussen AM
Investigative ophthalmology & visual science58(3)1387-1396(2017 03)

Structural decoding of netrin-4 reveals a regulatory function towards mature basement membranes.
Reuten R, Patel TR, McDougall M, Rama N, Nikodemus D, Gibert B, Delcros JG, Prein C, Meier M, Metzger S, Zhou Z, Kaltenberg J, McKee KK, Bald T, T?ting T, Zigrino P, Djonov V, Bloch W, Clausen-Schaumann H, Poschl E, Yurchenco PD, Ehrbar M, Mehlen P, Stetefeld J, Koch M
Nature communications713515(2016 Nov)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Netrin-4 acted as an antiangiogenic factor in the cornea, with which the healthy cornea is enriched via its expression by corneal epithelial cells.
Title: Netrin-4 Mediates Corneal Hemangiogenesis but Not Lymphangiogenesis in the Mouse-Model of Suture-Induced Neovascularization.

Our results indicate a role for netrin-4 as an angiogenesis modulating factor in O2-dependent vascular homeostasis while being less important during normal retinal developmental angiogenesis or during inflammatory neovascularization.
Title: Lack of netrin-4 modulates pathologic neovascularization in the eye.

These results indicate that netrin-4 shed new light on its potential roles in treatmenting for angiogenic diseases that affect the ocular surface, as well as other tissues
Title: Therapeutic effects of topical netrin-4 inhibits corneal neovascularization in alkali-burn rats.

Vascular endothelial cells interact with bone in suppressing bone through netrin-4.
Title: Netrin-4 derived from murine vascular endothelial cells inhibits osteoclast differentiation in vitro and prevents bone loss in vivo.

Loss of Netrin-4 is associated with metastatic colorectal tumor progression.
Title: Netrin-4 overexpression suppresses primary and metastatic colorectal tumor progression.

The following Ntn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ntn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12172	NM_021320.3 Latest version!	Mus musculus netrin 4 (Ntn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ntn4 ( NM_021320.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067295.2 Mus musculus netrin 4 (Ntn4), mRNA.

Price & Availability↑

CloneID	OMu12172
Clone ID Related Accession (Same CDS sequence)	NM_021320.3
Accession Version	NM_021320.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	netrin-4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ068258.1, BB609851.1, BC119182.1, CX205916.1, CF537120.1 and AC151976.3. On Apr 9, 2009 this sequence version replaced NM_021320.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF281278.1, BC119182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAGCT GCGCACGGCT GCTGCTGCTC TGGGGCTGCT CCGCGGTGGC CGCAGGCTTG 
AATGGAGTAG CCGGAGCGAA CTCCCGCTGT GAGAAAGCAT GCAACCCTCG CATGGGAAAC 
TTGGCTTTGG GAAGAAAGCT CCGGGCAGAC ACAATGTGTG GCCAGAACGC CACCGAACTC 
TTCTGCTTCT ACAGTGAGAA TGCTGACCTC ACTTGCCGGC AGCCCAAGTG TGATAAATGC 
AACGCTGCCC ATTCTCACCT GGCTCACCCA CCCTCTGCCA TGGCAGACTC ATCCTTCAGG 
TTTCCCCGGA CATGGTGGCA GTCTGCAGAG GATGTGCACA GGGAAAAGAT TCAGCTAGAC 
CTGGAAGCAG AATTCTACTT CACTCACCTA ATTATGGTGT TCAAGTCTCC CAGGCCTGCA 
GCCATGGTGC TGGACCGGTC CCAGGACTTT GGGAAGACCT GGAAGCCTTA CAAGTACTTT 
GCAACAAACT GCTCGGCTAC TTTTGGCCTG GAAGATGATG TTGTCAAGAA GGGAGCTATT 
TGCACGTCTA GATACTCAAA TCCTTTCCCG TGCACCGGAG GAGAGGTTAT TTTCAGAGCC 
CTGTCACCAC CATACGACAT AGAAAACCCT TACAGTGCCA AAGTGCAGGA GCAGCTGAAG 
ATCACCAACC TCCGAGTGCG GCTGCTCAAG CGACAGTCCT GCCCTTGTCA GATAAACGAC 
CTGAACGCAA AACCTCACCA TTTTATGCAC TACGCAGTCT ATGACTTCAT CGTCAAGGGC 
AGCTGCTTCT GCAACGGCCA CGCTGACCAG TGCTTACCTG TGGAGGGCTT CAGACCCATC 
AAGGCCCCGG GAGCGTTCCA CGTGGTCCAC GGGAGGTGTA TGTGTAAGCA CAACACAGCA 
GGCAGCCACT GCCAGCACTG TGCACCATTG TACAATGACC GGCCCTGGGA GGCAGCGGAC 
GGCAGAACAG GGGCTCCTAA CGAATGCAGA ACTTGCAAGT GCAATGGGCA CGCGGACACC 
TGTCACTTCG ACGTCAACGT GTGGGAGGCG TCGGGGAACC GCAGCGGCGG TGTCTGCAAC 
AACTGTCAGC ACAACACTGA GGGTCAGCAC TGTCAACGCT GTAAGCCCGG TTTCTACCGC 
GACCTCAGAA GACCCTTCTC TGCCCCTGAC GCTTGCAAAG CGTGTTCCTG CCACCCGGTT 
GGATCGGCGA TCCTTCCTTT CAGCTCAGTG ACCTTCTGCG ACCCCAGCAA TGGTGACTGC 
CCCTGCAAGC CTGGGGTGGC GGGGCCACAT TGCGACAGAT GCATGGTGGG ATACTGGGGC 
TTTGGAGACT ACGGCTGCAG ACCTTGCGAT TGTGCGGGGA GCTGCGACCC GCTCACGGGA 
GACTGCATCA GCAGTAACGC TGATGTAGAC TGGTACCACG AAGTCCCCGC CTTTCACTCG 
ATGCACAATA AGAGTGAGCC CAGCTGGGAA TGGGAGGATG AGCAAGGATT TTCTGCCCTC 
CGACACTCAG GTAAATGTGA ATGTAAGGAA CAGGTGTTAG GAAACCCCAA AGCCTTCTGT 
GGAATGAAGT ATTCATATGT GTTAAAAATC AAGATCTTAT CAGCCCACGA CAAAGGCTCC 
CATGCCGAAG TCAATGTGAA GATTAAGAAA GTCTTAAAGT CCACCAAACT GAAGATCTTA 
CGAGGCAAGA GAACGCTATA CCCAGAGTCC TGGACTAACA GAGGCTGCAC CTGTCCAATC 
CTCAATCCAG GATTGGAGTA CCTGGTCGCT GGCCACGAGG ACGTAAGAAC AGGCAAATTA 
ATTGTGAATA TGAAAAGCTT TGTCCAGCAC TGGAAACCAG CTCTTGGCAG AAGAGTCATG 
CACATCTTAA AAAGAGACTG CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067295.2
CDS	406..2292
Translation	MGSCARLLLLWGCSAVAAGLNGVAGANSRCEKACNPRMGNLALGRKLRADTMCGQNATELFCFYSENADLTCRQPKCDKCNAAHSHLAHPPSAMADSSFRFPRTWWQSAEDVHREKIQLDLEAEFYFTHLIMVFKSPRPAAMVLDRSQDFGKTWKPYKYFATNCSATFGLEDDVVKKGAICTSRYSNPFPCTGGEVIFRALSPPYDIENPYSAKVQEQLKITNLRVRLLKRQSCPCQINDLNAKPHHFMHYAVYDFIVKGSCFCNGHADQCLPVEGFRPIKAPGAFHVVHGRCMCKHNTAGSHCQHCAPLYNDRPWEAADGRTGAPNECRTCKCNGHADTCHFDVNVWEASGNRSGGVCNNCQHNTEGQHCQRCKPGFYRDLRRPFSAPDACKACSCHPVGSAILPFSSVTFCDPSNGDCPCKPGVAGPHCDRCMVGYWGFGDYGCRPCDCAGSCDPLTGDCISSNADVDWYHEVPAFHSMHNKSEPSWEWEDEQGFSALRHSGKCECKEQVLGNPKAFCGMKYSYVLKIKILSAHDKGSHAEVNVKIKKVLKSTKLKILRGKRTLYPESWTNRGCTCPILNPGLEYLVAGHEDVRTGKLIVNMKSFVQHWKPALGRRVMHILKRDCV

Target ORF information:

RefSeq Version	NM_021320.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus netrin 4 (Ntn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021320.3

ORF Insert Sequence:

ATGGGGAGCT GCGCACGGCT GCTGCTGCTC TGGGGCTGCT CCGCGGTGGC CGCAGGCTTG 
AATGGAGTAG CCGGAGCGAA CTCCCGCTGT GAGAAAGCAT GCAACCCTCG CATGGGAAAC 
TTGGCTTTGG GAAGAAAGCT CCGGGCAGAC ACAATGTGTG GCCAGAACGC CACCGAACTC 
TTCTGCTTCT ACAGTGAGAA TGCTGACCTC ACTTGCCGGC AGCCCAAGTG TGATAAATGC 
AACGCTGCCC ATTCTCACCT GGCTCACCCA CCCTCTGCCA TGGCAGACTC ATCCTTCAGG 
TTTCCCCGGA CATGGTGGCA GTCTGCAGAG GATGTGCACA GGGAAAAGAT TCAGCTAGAC 
CTGGAAGCAG AATTCTACTT CACTCACCTA ATTATGGTGT TCAAGTCTCC CAGGCCTGCA 
GCCATGGTGC TGGACCGGTC CCAGGACTTT GGGAAGACCT GGAAGCCTTA CAAGTACTTT 
GCAACAAACT GCTCGGCTAC TTTTGGCCTG GAAGATGATG TTGTCAAGAA GGGAGCTATT 
TGCACGTCTA GATACTCAAA TCCTTTCCCG TGCACCGGAG GAGAGGTTAT TTTCAGAGCC 
CTGTCACCAC CATACGACAT AGAAAACCCT TACAGTGCCA AAGTGCAGGA GCAGCTGAAG 
ATCACCAACC TCCGAGTGCG GCTGCTCAAG CGACAGTCCT GCCCTTGTCA GATAAACGAC 
CTGAACGCAA AACCTCACCA TTTTATGCAC TACGCAGTCT ATGACTTCAT CGTCAAGGGC 
AGCTGCTTCT GCAACGGCCA CGCTGACCAG TGCTTACCTG TGGAGGGCTT CAGACCCATC 
AAGGCCCCGG GAGCGTTCCA CGTGGTCCAC GGGAGGTGTA TGTGTAAGCA CAACACAGCA 
GGCAGCCACT GCCAGCACTG TGCACCATTG TACAATGACC GGCCCTGGGA GGCAGCGGAC 
GGCAGAACAG GGGCTCCTAA CGAATGCAGA ACTTGCAAGT GCAATGGGCA CGCGGACACC 
TGTCACTTCG ACGTCAACGT GTGGGAGGCG TCGGGGAACC GCAGCGGCGG TGTCTGCAAC 
AACTGTCAGC ACAACACTGA GGGTCAGCAC TGTCAACGCT GTAAGCCCGG TTTCTACCGC 
GACCTCAGAA GACCCTTCTC TGCCCCTGAC GCTTGCAAAG CGTGTTCCTG CCACCCGGTT 
GGATCGGCGA TCCTTCCTTT CAGCTCAGTG ACCTTCTGCG ACCCCAGCAA TGGTGACTGC 
CCCTGCAAGC CTGGGGTGGC GGGGCCACAT TGCGACAGAT GCATGGTGGG ATACTGGGGC 
TTTGGAGACT ACGGCTGCAG ACCTTGCGAT TGTGCGGGGA GCTGCGACCC GCTCACGGGA 
GACTGCATCA GCAGTAACGC TGATGTAGAC TGGTACCACG AAGTCCCCGC CTTTCACTCG 
ATGCACAATA AGAGTGAGCC CAGCTGGGAA TGGGAGGATG AGCAAGGATT TTCTGCCCTC 
CGACACTCAG GTAAATGTGA ATGTAAGGAA CAGGTGTTAG GAAACCCCAA AGCCTTCTGT 
GGAATGAAGT ATTCATATGT GTTAAAAATC AAGATCTTAT CAGCCCACGA CAAAGGCTCC 
CATGCCGAAG TCAATGTGAA GATTAAGAAA GTCTTAAAGT CCACCAAACT GAAGATCTTA 
CGAGGCAAGA GAACGCTATA CCCAGAGTCC TGGACTAACA GAGGCTGCAC CTGTCCAATC 
CTCAATCCAG GATTGGAGTA CCTGGTCGCT GGCCACGAGG ACGTAAGAAC AGGCAAATTA 
ATTGTGAATA TGAAAAGCTT TGTCCAGCAC TGGAAACCAG CTCTTGGCAG AAGAGTCATG 
CACATCTTAA AAAGAGACTG CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Gastroenterology(2017 Oct)
Memic F,Knoflach V,Morarach K,Sadler R,Laranjeira C,Hjerling-Leffler J,Sundstr?m E,Pachnis V,Marklund U

Netrin-4 Promotes Differentiation and Migration of Osteoblasts.
In vivo (Athens, Greece)31(5)793-799(2017 Sep-Oct)
Enoki Y,Sato T,Kokabu S,Hayashi N,Iwata T,Yamato M,Usui M,Matsumoto M,Tomoda T,Ariyoshi W,Nishihara T,Yoda T

Netrin-4 Mediates Corneal Hemangiogenesis but Not Lymphangiogenesis in the Mouse-Model of Suture-Induced Neovascularization.
Investigative ophthalmology & visual science58(3)1387-1396(2017 03)
Maier AB,Klein S,Kociok N,Riechardt AI,Gundlach E,Reichhart N,Strau? O,Joussen AM

Structural decoding of netrin-4 reveals a regulatory function towards mature basement membranes.
Nature communications713515(2016 Nov)
Reuten R,Patel TR,McDougall M,Rama N,Nikodemus D,Gibert B,Delcros JG,Prein C,Meier M,Metzger S,Zhou Z,Kaltenberg J,McKee KK,Bald T,T?ting T,Zigrino P,Djonov V,Bloch W,Clausen-Schaumann H,Poschl E,Yurchenco PD,Ehrbar M,Mehlen P,Stetefeld J,Koch M

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Ntn4 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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