kiss1ra cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kiss1ra
Entrez Gene ID	561898
Full Name	KISS1 receptor a
Synonyms	gpr54,gpr54-1,gpr54a,kiss1r,kiss1rl,kissr2,si:dkey-31m5.9
General protein information	Preferred Names KISS1 receptor a Names KISS1 receptor a G protein-coupled receptor 54 KISS1 receptor like kisspeptin receptor 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105679.2	NP_001099149.2	KISS1 receptor a

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	kissr2	NP_001165296.1
Danio rerio (zebrafish)	kiss1ra	NP_001099149.1
Drosophila melanogaster (fruit fly)	AR-2	NP_524544.1

Gene Ontology
Bibliography

Related articles in PubMed

The gonadotropin-inhibitory hormone (Lpxrfa) system's regulation of reproduction in the brain-pituitary axis of the zebrafish (Danio rerio).
Spicer OS, Zmora N, Wong TT, Golan M, Levavi-Sivan B, Gothilf Y, Zohar Y
Biology of reproduction96(5)1031-1042(2017 May)

Linking the response of endocrine regulated genes to adverse effects on sex differentiation improves comprehension of aromatase inhibition in a Fish Sexual Development Test.
Muth-K?hne E, Westphal-Settele K, Br?ckner J, Konradi S, Schiller V, Sch?fers C, Teigeler M, Fenske M
Aquatic toxicology (Amsterdam, Netherlands)176116-27(2016 Jul)

Early-life stress changes expression of GnRH and kisspeptin genes and DNA methylation of GnRH3 promoter in the adult zebrafish brain.
Khor YM, Soga T, Parhar IS
General and comparative endocrinology22784-93(2016 Feb)

Actions of Bisphenol A and Bisphenol S on the Reproductive Neuroendocrine System During Early Development in Zebrafish.
Qiu W, Zhao Y, Yang M, Farajzadeh M, Pan C, Wayne NL
Endocrinology157(2)636-47(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

not absolutely required for zebrafish reproduction
Title: The kiss/kissr systems are dispensable for zebrafish reproduction: evidence from gene knockout studies.

duplicated kiss1r genes evolved a distinct gene expression pattern, different ligand selectivity, and novel nuclear isoforms. While a single kiss1ra mRNA was detected exclusively in the brain, 5 kiss1rb transcripts were found in many peripheral tissues.
Title: Duplicated Kiss1 receptor genes in zebrafish: distinct gene expression patterns, different ligand selectivity, and a novel nuclear isoform with transactivating activity.

The only site of expression of kiss1r mRNA cells was the ventromedial habenula, showing an expression pattern very similar to that of the kiss1 mRNA or proKiss1 peptide.
Title: Organization of two independent kisspeptin systems derived from evolutionary-ancient kiss genes in the brain of zebrafish.

Kiss1/Kiss1r system may participate in puberty initiation in fish as well.
Title: Molecular identification and functional characterization of the kisspeptin/kisspeptin receptor system in lower vertebrates.

Zebrafish kiss1r was expressed predominantly in the brain, with a minor level of expression in the eye.
Title: Evidence for the existence of a functional Kiss1/Kiss1 receptor pathway in fish.

The following kiss1ra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kiss1ra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa07153	NM_001105679.2 Latest version!	Danio rerio KISS1 receptor a (kiss1ra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kiss1ra ( NM_001105679.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001099149.2 Danio rerio KISS1 receptor a (kiss1ra), mRNA.

Price & Availability↑

CloneID	ODa07153
Clone ID Related Accession (Same CDS sequence)	NM_001105679.2
Accession Version	NM_001105679.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	KISS1 receptor a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649508.10 and EF672263.1. On or before May 29, 2015 this sequence version replaced XM_005163499.2, NM_001105679.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB623211.1, EU047917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476770, SAMEA4476776 [ECO:0000348] ##Evidence-Data-END##

ATGTTTTCTG GTGAAGACTG GAATTCTAGT GAGCTTCTGA ATGGCTCTTT CAGAAACTCT 
TCTATGGAGG ATTCGGAGGA CGGGGAGCAC CCGTTCCTGA CAGATGCATG GCTTGTGCCG 
CTCTTTTTCT CTCTCATCAT GCTAGTGGGG CTGATCGGAA ACTCACTGGT GATTTATGTC 
ATCTCCAAAC ACAGACAGAT GAGGACGGCT ACTAACTTTT ACATAGCTAA CTTGGCTGCC 
ACCGACATCA TTTTTCTGCT GTGCTGTGTG CCGTTCACCG CCACCCTCTA CCCTCTGCCC 
GGCTGGATAT TTGGGGACTT CATGTGCAAA TTTGTTGCTT TTCTTCAACA GGTGACGGTA 
CAGGCGACGT GCATCACTCT CACGGCCATG AGTGGAGACC GATGTTATGT GACTGTGTAT 
CCTCTCAAAT CCCTGCACCA TCGCACCCCT CGTGTTGCTA TGATTGTTAG CATATGCATA 
TGGATTGGTT CCTTCATTCT TTCCATACCG ATCTTCCTGT ACCAGAGGCT GGAAGACGGC 
TATTGGTATG GACCAAGAAA ATACTGCATG GAAAGGTTTC CATCAAAGAC CCATGAGAAA 
GCTTTCATCC TCTATCAGTT CATAGCTGTG TATCTACTGC CTGTCATTAC CATCTCTTTC 
TGTTATTCCT TCATGTTGAA AAGAGTGGGA CAGGCTTCGG TGGAACCAGT GGATAACAAC 
CATCAGGTCC ATCTTCTCTC AGAGAGAACC ATCTCCATCC GGAGCAAGAT ATCCAAAATG 
GTAGTGGTCA TAGTTGTCCT CTTCACCATC TGCTGGGGCC CCATTCAGAT CTTTGTTCTG 
TTCCAATCCT TCTATCCTAA CTTCAAGGCC AACTACGCAA CATATAAGAT CAAAACGTGG 
GCCAACTGCA TGTCCTATGC CAATTCCTCT ATCAACCCCA TTGTCTACGG CTTTATGGGC 
GCCAGCTTCC GCAAGTCTTT CAGGAAGACC TTTCCCTTCC TCTTCAGACA CAAAGTGCGG 
GACAGCAGCG TGGCCTCCCG CACGGCCAAT GCAGAAATCA AATTTGTAGC CACAGAGGAA 
AGCAACACTG AGAGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099149.2
CDS	1..1101
Translation	MFSGEDWNSSELLNGSFRNSSMEDSEDGEHPFLTDAWLVPLFFSLIMLVGLIGNSLVIYVISKHRQMRTATNFYIANLAATDIIFLLCCVPFTATLYPLPGWIFGDFMCKFVAFLQQVTVQATCITLTAMSGDRCYVTVYPLKSLHHRTPRVAMIVSICIWIGSFILSIPIFLYQRLEDGYWYGPRKYCMERFPSKTHEKAFILYQFIAVYLLPVITISFCYSFMLKRVGQASVEPVDNNHQVHLLSERTISIRSKISKMVVVIVVLFTICWGPIQIFVLFQSFYPNFKANYATYKIKTWANCMSYANSSINPIVYGFMGASFRKSFRKTFPFLFRHKVRDSSVASRTANAEIKFVATEESNTERK

Target ORF information:

RefSeq Version	NM_001105679.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio KISS1 receptor a (kiss1ra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105679.2

ORF Insert Sequence:

ATGTTTTCTG GTGAAGACTG GAATTCTAGT GAGCTTCTGA ATGGCTCTTT CAGAAACTCT 
TCTATGGAGG ATTCGGAGGA CGGGGAGCAC CCGTTCCTGA CAGATGCATG GCTTGTGCCG 
CTCTTTTTCT CTCTCATCAT GCTAGTGGGG CTGATCGGAA ACTCACTGGT GATTTATGTC 
ATCTCCAAAC ACAGACAGAT GAGGACGGCT ACTAACTTTT ACATAGCTAA CTTGGCTGCC 
ACCGACATCA TTTTTCTGCT GTGCTGTGTG CCGTTCACCG CCACCCTCTA CCCTCTGCCC 
GGCTGGATAT TTGGGGACTT CATGTGCAAA TTTGTTGCTT TTCTTCAACA GGTGACGGTA 
CAGGCGACGT GCATCACTCT CACGGCCATG AGTGGAGACC GATGTTATGT GACTGTGTAT 
CCTCTCAAAT CCCTGCACCA TCGCACCCCT CGTGTTGCTA TGATTGTTAG CATATGCATA 
TGGATTGGTT CCTTCATTCT TTCCATACCG ATCTTCCTGT ACCAGAGGCT GGAAGACGGC 
TATTGGTATG GACCAAGAAA ATACTGCATG GAAAGGTTTC CATCAAAGAC CCATGAGAAA 
GCTTTCATCC TCTATCAGTT CATAGCTGTG TATCTACTGC CTGTCATTAC CATCTCTTTC 
TGTTATTCCT TCATGTTGAA AAGAGTGGGA CAGGCTTCGG TGGAACCAGT GGATAACAAC 
CATCAGGTCC ATCTTCTCTC AGAGAGAACC ATCTCCATCC GGAGCAAGAT ATCCAAAATG 
GTAGTGGTCA TAGTTGTCCT CTTCACCATC TGCTGGGGCC CCATTCAGAT CTTTGTTCTG 
TTCCAATCCT TCTATCCTAA CTTCAAGGCC AACTACGCAA CATATAAGAT CAAAACGTGG 
GCCAACTGCA TGTCCTATGC CAATTCCTCT ATCAACCCCA TTGTCTACGG CTTTATGGGC 
GCCAGCTTCC GCAAGTCTTT CAGGAAGACC TTTCCCTTCC TCTTCAGACA CAAAGTGCGG 
GACAGCAGCG TGGCCTCCCG CACGGCCAAT GCAGAAATCA AATTTGTAGC CACAGAGGAA 
AGCAACACTG AGAGGAAATG A