vhl cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	vhl
Entrez Gene ID	791202
Full Name	von Hippel-Lindau tumor suppressor
Synonyms	zgc:158722
General protein information	Preferred Names von Hippel-Lindau tumor suppressor Names von Hippel-Lindau disease tumor suppressor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA Protein Name

NM_001080684.1 NP_001074153.1 von Hippel-Lindau disease tumor suppressor
Pathways from BioSystems

mRNA	Protein	Name
NM_001080684.1	NP_001074153.1	von Hippel-Lindau disease tumor suppressor

Homologies

	LOC701870	XP_001090152.2
Bos taurus (cattle)	VHL	NP_001103489.1
Mus musculus (house mouse)	Vhl	NP_033533.1
Xenopus tropicalis (tropical clawed frog)	vhl	NP_001016367.1
Gallus gallus (chicken)	VHL	XP_414447.3
Homo sapiens (human)	VHL	NP_000542.1
Canis lupus familiaris (dog)	VHL	NP_001008552.1
Rattus norvegicus (Norway rat)	Vhl	NP_434688.1
Danio rerio (zebrafish)	vhl	NP_001074153.1

Gene Ontology
Bibliography

Related articles in PubMed

The von Hippel-Lindau Gene Is Required to Maintain Renal Proximal Tubule and Glomerulus Integrity in Zebrafish Larvae.
van Rooijen E, van de Hoek G, Logister I, Ajzenberg H, Knoers NVAM, van Eeden F, Voest EE, Schulte-Merker S, Giles RH
Nephron(2018 Jan)

HIF1?-induced PDGFR? signaling promotes developmental HSC production via IL-6 activation.
Lim SE, Esain V, Kwan W, Theodore LN, Cortes M, Frost IM, Liu SY, North TE
Experimental hematology4683-95.e6(2017 Feb)

Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization.
Wild R, Klems A, Takamiya M, Hayashi Y, Str?hle U, Ando K, Mochizuki N, van Impel A, Schulte-Merker S, Krueger J, Preau L, le Noble F
Nature communications813991(2017 Jan)

Zebrafish as a model for von Hippel Lindau and hypoxia-inducible factor signaling.
Kim HR, Greenald D, Vettori A, Markham E, Santhakumar K, Argenton F, van Eeden F
Methods in cell biology138497-523(2017)

Forkhead Transcription Factor 3a (FOXO3a) Modulates Hypoxia Signaling via Up-regulation of the von Hippel-Lindau Gene (VHL).
Liu X, Cai X, Hu B, Mei Z, Zhang D, Ouyang G, Wang J, Zhang W, Xiao W
The Journal of biological chemistry291(49)25692-25705(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this review, we have tried to bring together knowledge on the HIF/hypoxic signaling pathway in zebrafish, including what is known on VHL functions.
Title: Zebrafish as a model for von Hippel Lindau and hypoxia-inducible factor signaling.

by modulating hypoxia-inducible factor activity via up-regulation of VHL, FOXO3a (foxo3b) plays an important role in survival in response to hypoxic stress.
Title: Forkhead Transcription Factor 3a (FOXO3a) Modulates Hypoxia Signaling via Up-regulation of the von Hippel-Lindau Gene (VHL).

the vhl(-/-) zebrafish kidney is characterized by an increased tubule diameter, disorganized cilia, the dramatic formation of cytoplasmic lipid vesicles, glycogen accumulation, aberrant cell proliferation and abnormal apoptosis.
Title: Loss of vhl in the zebrafish pronephros recapitulates early stages of human clear cell renal cell carcinoma.

Zebrafish embryos lacking endogenous vhl lead to defective phenotypes in the renal system that were characterized by the curved and cystic pronephric tubule or/and a cystic and malformed glomerulus.
Title: von Hippel-Lindau gene plays a role during zebrafish pronephros development.

Inhibiting PHD or knocking down VHL rescues Methyl tert-butyl ether induced vascular lesions.
Title: Manipulation of the HIF-Vegf pathway rescues methyl tert-butyl ether (MTBE)-induced vascular lesions.

The following vhl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vhl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00072	NM_001080684.1 Latest version!	Danio rerio von Hippel-Lindau tumor suppressor (vhl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vhl ( NM_001080684.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001074153.1 Danio rerio von Hippel-Lindau tumor suppressor (vhl), mRNA.

Price & Availability↑

CloneID	ODa00072
Clone ID Related Accession (Same CDS sequence)	NM_001080684.1
Accession Version	NM_001080684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Danio rerio(zebrafish)
Product	von Hippel-Lindau disease tumor suppressor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129410.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC129410.1, CO917290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCCCAGG ACTCTCAGGA GGGGCAGCAG CCGCTCCCGC TGGTCCGGTC TCTGATCAGC 
CGGATACAGG TCAACGTTCT GTTCTGTAAC TGCAGCCCGC GCGTCGTCAA GCCCGTCTGG 
ATCAACTTCC TCGGAGAGCC GCAGCCGTAC GTGAACATTC AGCCGTACAC CGGACGAAGA 
ATAACAACTT TTGTGGGACA TCCATGGATG TTTCGGGATG CAGAAACTGA TGATCCAATG 
GTAGTCAACA ATAAAGAGAT GTATTTGCCA GCATCTCTTG AAAATGGGCA AGTCGCAAAT 
GCCAAGATCA CATTGCCTGT GCTGACGTTG CGAGATCGCT GTCTGCAAGT TGTGAGACGG 
TTGGTGCGCA GGGAAGATGT CGGCCGTCTG GAGATTGCCC GCTGCCTTCA AGAGGATCTA 
GCTCAGAGAC CCAGCATTCA GGCTGATCTA CGACGCATTA GCCAGCGGGT GGAACAGAAG 
TTACTGGAGA ACAGAGAACT GCAAAACAGA CGAAAACATC AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074153.1
CDS	253..780
Translation	MPQDSQEGQQPLPLVRSLISRIQVNVLFCNCSPRVVKPVWINFLGEPQPYVNIQPYTGRRITTFVGHPWMFRDAETDDPMVVNNKEMYLPASLENGQVANAKITLPVLTLRDRCLQVVRRLVRREDVGRLEIARCLQEDLAQRPSIQADLRRISQRVEQKLLENRELQNRRKHQH

Target ORF information:

RefSeq Version	NM_001080684.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio von Hippel-Lindau tumor suppressor (vhl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080684.1

ORF Insert Sequence:

ATGCCCCAGG ACTCTCAGGA GGGGCAGCAG CCGCTCCCGC TGGTCCGGTC TCTGATCAGC 
CGGATACAGG TCAACGTTCT GTTCTGTAAC TGCAGCCCGC GCGTCGTCAA GCCCGTCTGG 
ATCAACTTCC TCGGAGAGCC GCAGCCGTAC GTGAACATTC AGCCGTACAC CGGACGAAGA 
ATAACAACTT TTGTGGGACA TCCATGGATG TTTCGGGATG CAGAAACTGA TGATCCAATG 
GTAGTCAACA ATAAAGAGAT GTATTTGCCA GCATCTCTTG AAAATGGGCA AGTCGCAAAT 
GCCAAGATCA CATTGCCTGT GCTGACGTTG CGAGATCGCT GTCTGCAAGT TGTGAGACGG 
TTGGTGCGCA GGGAAGATGT CGGCCGTCTG GAGATTGCCC GCTGCCTTCA AGAGGATCTA 
GCTCAGAGAC CCAGCATTCA GGCTGATCTA CGACGCATTA GCCAGCGGGT GGAACAGAAG 
TTACTGGAGA ACAGAGAACT GCAAAACAGA CGAAAACATC AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The von Hippel-Lindau Gene Is Required to Maintain Renal Proximal Tubule and Glomerulus Integrity in Zebrafish Larvae.
Nephron(2018 Jan)
van Rooijen E,van de Hoek G,Logister I,Ajzenberg H,Knoers NVAM,van Eeden F,Voest EE,Schulte-Merker S,Giles RH

HIF1?-induced PDGFR? signaling promotes developmental HSC production via IL-6 activation.
Experimental hematology4683-95.e6(2017 Feb)
Lim SE,Esain V,Kwan W,Theodore LN,Cortes M,Frost IM,Liu SY,North TE

Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization.
Nature communications813991(2017 Jan)
Wild R,Klems A,Takamiya M,Hayashi Y,Str?hle U,Ando K,Mochizuki N,van Impel A,Schulte-Merker S,Krueger J,Preau L,le Noble F

Zebrafish as a model for von Hippel Lindau and hypoxia-inducible factor signaling.
Methods in cell biology138497-523(2017)
Kim HR,Greenald D,Vettori A,Markham E,Santhakumar K,Argenton F,van Eeden F

Forkhead Transcription Factor 3a (FOXO3a) Modulates Hypoxia Signaling via Up-regulation of the von Hippel-Lindau Gene (VHL).
The Journal of biological chemistry291(49)25692-25705(2016 Dec)
Liu X,Cai X,Hu B,Mei Z,Zhang D,Ouyang G,Wang J,Zhang W,Xiao W

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vhl cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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