cacna1fa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1fa
Entrez Gene ID	793708
Full Name	calcium channel, voltage-dependent, L type, alpha 1F subunit a
Synonyms	si:ch73-289n4.1
General protein information	Preferred Names calcium channel, voltage-dependent, L type, alpha 1F subunit a Names voltage-dependent L-type calcium channel subunit alpha-1D calcium channel, voltage-dependent, L type, alpha 1F subunit-like wait until dark wud
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357598.1	XP_017213087.1	voltage-dependent L-type calcium channel subunit alpha-1D
XM_021478483.1	XP_021334158.1	voltage-dependent L-type calcium channel subunit alpha-1D

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1F	NP_005174.2
	CACNA1F	XP_001106163.2
Canis lupus familiaris (dog)	CACNA1F	XP_005642146.1
Danio rerio (zebrafish)	LOC559964	XP_003199134.2
Bos taurus (cattle)	CACNA1F	NP_001192592.1
Mus musculus (house mouse)	Cacna1f	NP_062528.2
Danio rerio (zebrafish)	LOC793708	XP_001333514.5
Xenopus tropicalis (tropical clawed frog)	cacna1f	XP_002941018.2
Rattus norvegicus (Norway rat)	Cacna1f	NP_446153.1

Gene Ontology

The following cacna1fa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1fa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa46831	XM_021478483.1 Latest version!	Danio rerio calcium channel, voltage-dependent, L type, alpha 1F subunit a (cacna1fa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa60181	XM_017357598.1 Latest version!	Danio rerio calcium channel, voltage-dependent, L type, alpha 1F subunit a (cacna1fa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1fa ( XM_021478483.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334158.1 Danio rerio calcium channel, voltage-dependent, L type, alpha 1F subunit a (cacna1fa), mRNA.

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CloneID	ODa46831
Clone ID Related Accession (Same CDS sequence)	XM_021478483.1
Accession Version	XM_021478483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent L-type calcium channel subunit alpha-1D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGG CAACGAAAGA TGGGGTTGGC TTGGACCCCA GCAAAACGGA CACACTCAAC 
TCAACAGGTT CTGCAGGACA AAAAAGAGCT GGAGGAGCCA AGAAGCACAC CCAAGCTAAC 
AAATCAGCCT TGCGTGCACC CAGAGCTCTG TGCTGCCTTA CTCTCAGCAA TCCTATTCGC 
ATGGCAGCTC TGGCTCTAGT GGAGTGGAAG CCATTTGATA TATTCATTTT ACTGGCCATC 
TTCGCAAATT GTGTTGCTCT TGGTGTGTCC AAACCGTTTC CTGAGGATGA TTCCAATGCC 
ACCAACCACA ATCTGGAACA AGTGGAGTAT GTGTTTCTGG TCATTTTCAC CATCGAAACT 
TTCACAAAGA TTCTGGCATA TGGTCTGGTC ATGCATCCCA GTGCCTACAT CCGCAGCGGG 
TGGAACTTGC TGGATTTTAT CATTGTCATT GTTGGTCTTT TCAGTGTGAT AGCTGAAATG 
GGAGGAGACG GTCAAAAAGC AGACAGCCAT CATGCAGCCG GTAAACCAGG TGGTCTGGAT 
GTCAAAGCCC TGAGAGCTTT TAGAGTTTTG CGACCACTTC GTCTAGTGTC TGGTGTGCCG 
AGTCTGCAGA TTGTGTTAAA CTCGATCATG AAGGCCATGG TGCCTCTGCT CCATATTGGT 
CTGCTCGTCA TGTTTGTCAT CATCATATAT GCAATCATTG GTCTGGAGCT TTTTATAGGC 
AGGATGCACA AGACCTGCTT TTATGTAGGG ACAGAACTTA TGGTGGAGGA TGATCCAACA 
CCATGTGCTT TTGCCGGTCA CGGACGCGTC TGTATAGGCA ATAACACAGA TTGTCGTGGA 
GGATGGGAGG GACCAAATGG AGGCATCACC AACTTTGATA ATATCTTCTT TGCCATGCTC 
ACTGTGTTCC AGTGTATTAC AATGGAGGGC TGGACTGATG TCCTTTACTG GATGAACGAT 
TCTATTGGGT TTGAGTTGCC TTGGGTTTAT TTTGTGTCTC TGGTCATTTT TGGTTCATTC 
TTTGTCCTGA ATCTTGTATT GGGTGTGCTG AGCGGCGAGT TCTCTAAAGA GAGAGAGAAG 
GCAGTGTGTC GCGGTGAGCT GCAGAAGGCT CAGGAGAAAC AGCAGATGGA GGAGGACATG 
ATCGGCTACA TGGACTGGCT CATTGAAGCT GAGGATATGG ATGAAGATGG AAATAAGCGT 
GCTGTGGTGG CCAAGAAAAA GATGATGAAG AAATATGGCT GGTACAAGCA TAGTGAAGAT 
GGAGAATCTG ATTCTGATTC GGAGTTTGAA GCATTCCTGG ATGATGACAA TGGCTGCTGT 
GCTTCCATAA TGGCACGACT GATGGCTATA AGTTTCTGTG AGCAACTGTA CCACTTGAAT 
GTAGTTATGA GGAAGAACTG TCGGGTTGCA GTCAAATCCA CCAATTTCTA CTGGCTGGTG 
CTACTACTAG TGTTCCTCAA CACTGCTGCC AGCGCTTCTG AACATTATGG CCAGCCCAAG 
TGGCTCACTG ACATACAGGA ACGAGCAAAT AAGATCCTTC TGGCTCTGTT TACGCTGGAG 
ATGCTGATGA AGATTTACAG CTTCGGGTTT CAGATCTACT TCATGGCACT CTTCAATCGT 
TTTGATTGTT TCGTGGTGTG TGGTGGAATT CTGGAAACTG TGCTTGTGGA GATGGAGGTG 
ATCCCACCCA TTGGAATCTC TGTGCTGCGC TGTGTCCGTC TGCTCCGTAT CTTCAAAGTC 
ACAAGACACT GGGCTGCCCT CTCAGACCTG GTGGGCTCTT TGCTGAACTC TATGAAGGCC 
ATTTGCTCCC TGCTGTTGCT GCTATTCCTC TTCCTCATTA TTTTTGCTCT CCTGGGAATG 
CAGCTTTTCG GTGGAAAGTT CAACTTTGAT GAGACTCAAA TGAAAAGAAG CACCTTCGAC 
TCTTTTCCAT CTGCCTTACT CACCTGTTTC CAGATCCTAA CAGGAGAGGA CTGGAACTCC 
GTCATGTATG ATGGCATCAT GGCTTATGGT GGACCAGTGT TTCCCAATAT GATTGTCTGC 
ATTTACTTTG TCATTCTTTT CGTCTGTGGT AACTACATCT TACTGAACGT CTTCTTGGCT 
ATTGCTGTGG ATAACTTGGC TGGAGATGGT GGCAAAAAGA AAAAAGAAGA GAAAAAGGAA 
GAGGAGGAGG AATGGGAAGA TGAAGATGAA AGAGAGGAAG ATGATGCTGG GAATGAGATG 
GATGATTGGG AAGAGAATGA AGAGCTGAGA GCAATCGAAG GGCTTGAGGG TATAGGCACT 
CCAATGAAGG TCGAAAGTTT CCAACCCAAA GAGAAAATTG TGCCTATTCC TGATGGCAGT 
TCCTTTTTTG TCCTTGGAAA GAAAAACTGT CTCAGAGTCG CCTGCCACAA CCTCATTCAT 
CATCATTACT TCACCAACTT CATCCTCATC TTCATTATAC TTAGTAGTTT TTCCTTGGCT 
GCTGAAGACC CAATCAAAAC ACATTCTGTC AGGAATGTTA TGCTGGGCTA TGCTGACTAC 
GTCTTTACCA CAGTTTTCAC TATTGAGATC ATGTTGAAGA TGACTGTGTA TGGAGCTTTC 
CTTCATCAAG GTTCCTTCTG CAGAAATGCC TTCAACCTTC TGGACCTTCT TGTCGTCAGT 
GTGTCCCTCA CCTCTTTCTT TTTGAATTCG AGTGCCATTT CTGTGGTGAA GATTCTTAGA 
GTTTTGCGAG TGCTTAGGCC TCTTCGAGCC ATCAACAGGG CAAAGGGGCT CAAGAGCGTG 
ATTCAGTGTG TATTTGTGGC CATCCGCACT ATTGGCAACA TCCTCATTGT CACCACTCTT 
CTCCAGTTCA TGTTCGCTTG TATTGGTGTC CAGCTTTTTA AGGGCCGGTT CTATAGATGC 
ACAGATGAAG CGAAGCATAC ACCTGAAGAG TGCAAGGGCA CTTTTGTTGT GTTCAAAGAT 
GGTGATATGA ACCATCCAAT GGTGAGGGAG AGAGTTTGGG AAAACAGCGA GTTTAACTTT 
GACAATGTTC TGATGGGCAT GCTTGCGCTC TTCACTGTCT CTACATTTGA GGGCTGGCCA 
CAGCTCTTGT ACAAAGCCAT TGATGCCAAT GCAGAGAACA GAGGACCCAT CTATAACTAC 
CGTGTAGAGA TTTCCATCTT CTTCATCATC TACATTATCA TCATTGCCTT CTTCATGATG 
AACATCTTTG TGGGTTTTGT CATTATCACA TTTCGTGAAC AAGGAGAGGC AGAGTTTAAA 
AACTGTGAAC TAAATAAAAA TCAGCGTCAG TGCGTGTATT ATGCACTGAA AGCTCAACCA 
ATAAAGATTT ACATTCCCAA AAACCCTTCC CAACTTAAAT TTTGGAAGAT CATAAATTCC 
TCTCAGTTCG AGTATATCAT GTTTGTCCTC ATTCTGCTCA ACACACTCAC GTTGGCTGTA 
CAGCATTATG AGCAATCCAA ACTCTTCAAC TCTGTGATGG ACATACTGAA CATGATCTTC 
ACGACGCTCT TTACTGTTGA GATGATCATC AAACTCATGG CTCTGAGACC TTATCATTAT 
TTTATAGATG CCTGGAATTC TTTTGATGCA CTGATAGTGG TGGGCAGTTT GGTGGACATC 
ATGATTGCAG AGCTGAGCGG TGGTGGAGGC CATGGTGAGG GAAAAGAAGG CGAAAGTGCC 
AAAGTATCCA TCACTTTTTT CCGTCTTTTC CGTGTTATGC GACTGGTCAA GCTGCTCAGC 
AAGGGTGAAG GCATCCGTAC ACTCCTTTGG ACTTTCGTTA AATCACTACA GGCTTTACCA 
TACGTTGGCC TACTCATTGC GATGATCTTT TTTATTTATG GTGTGATTGG CATGCAGATA 
TTTGGGAAGA TAGCTATAGA CGACGATACA GAGCTTAATC GGAACAACAA TTTCCAAACC 
TTTTTCATGG CTGTGCTTTT GCTGTTCAGG TGTGCAACAG GTGAACAGTG GCAGCAGATC 
ATGCTTGCAG CACTGCCGGG GCACCGGTGT GACCCAGAGT CTGATTTTGA GGCGGGGGAG 
GAGTTTACCT GCGGAAGCAA CCTGGCATAC CTCTATTTCA TCAGTTTCTT TGCACTCTGT 
GCCTTTTTGA TTATTAACTT GTTCATTGCT GTCATCATGG ATAACTTTGA GTATCTGACT 
AGAGACTGGA CTGTGCTTGG CACTCATCAC CTCGATGAGT TTAAACGAGT CTGGTCAGAT 
TACGATCCAG AGGCCACGGG ACGGATTAAA CATTTAGATG TAGTTACCAT GCTGCGCAGA 
ATTCAGCCTC CACTGGGGTT TGGAAAACTC TGTCCACATC GAGTGGCCTG TAGGAGACTG 
GTTGCTATGA ATGTTCCTCT CTATCCTGAT GGCACTGTGT CCTTCAACGC CACCCTATTT 
GCTCTTGTCA GAACTTCACT CAAGATTAAA ACAGATGGCC CCATTGATCA ACAGAATGAA 
GAGCTGAGAG CTATTATTAA AAAGATCTGG AAACGCACCA AACCCAAACT CCTAGATGAA 
GTTATACCTC CCCCTTCAGG CAATGAAGTG ACTGCAGGGA AGTTTTATGC CAGCTTTCTA 
ATTCAGGACT ACTTTAAAAA GTTTCGCAAG AGAAAGGAGA AAGAGAGGAA GAAGAAAGGC 
AAAGACAAAT CAGCCTCTCT CCAGGCTGGG TTACGCACAT TACAGGATCT TGCCCCAGAG 
ATGCGACTAG CTATGGCGAT GGAGGATGAG GAAGCACTGG AGGGGGAGAT GATGGAAGAT 
GAGGAGTTCT TTAGGAGTGA CAGCGTCTTC AGTGATGTTC CTCCTACACC AACTATGTCC 
ACCCCTAGAA GCACGCCTAT GCCCCCACAT CTGGATCAGA CCACAGTAAA CATCGAGCTT 
CCCCCAAGAA TACGCAATGG AAGCTTAATG CTGGAGGAAG CCCTGTTGGA CTCCCGTCCA 
GCCTCTGCCC TATCTGAAAA TGAAGTGATA GTTGAACAGC CTAACAGGTT GTCTCCCTTA 
CCCAGCAAGT GGACCTCCAG TCACAGTAAA CTTACCCCAT TGTGGATACT GAATACCAAC 
CCAAGACAAG GAGCACAGAA TGACAGCACT GGAGTTTGTG TCTATCCACC TTCCCCATCT 
CCGGATGTAG CCATGCAAAA TGGACAAGTC ACAGAAGGGG ATGTCGTTCC AGGAGGACAT 
GGAGGAGCAA CTATGGGAGG AGTCATGGAA GCGGGAGTTG TCCATGGGGT AGCAACAGAT 
GGAGTAGTCC ATGGGGGATC AATCATAGGA GAAGAAGTAG TCATGGGAGG AGGAGTCACA 
GAATTGACAT ACACTGCAGA CCAAGGTGGT GTGGGCAGTG GGGACCCTCA GACTGCAGTC 
TTACCTGAAC AACAGTGGTT TCCTGAAGTT CCGCCAACAC AAGCTGTAGT ACCTGATAGG 
ACCACTGAAT GTGTGGCTCC AGTTCAGCCC ATCAACCTTA ACGGCAATGG TTACCAAGGA 
AACATCCAAG ATGCTGGCAA CTACAATGGC AGTTTCCCTG GAAATGGCTA CAATGGGAAT 
GGTACCAATG GAACTGATTA TCCAGGCAAT CGTTGCTCCA GTAACGGTTA TAGCACCAAT 
GGCTACAATG GTAATGGTTA CAATAGTAAT GGATTCAATG GTAATGGATA CAACAATAAT 
GAATACAGTG GCAATGGTTT TGCTGCACGG CGAAGAATGC TTCCCCCAAC ACCATTAGGC 
CGGAAGCCAA ACTTTAACAT TCAGTGTTTG CGAAGGCAGG GCAGTAATGA GGATCTCCCA 
GTCCCTGGCA CATATCATCA AAACTCCCCA CCCTGCAGGG CTCGCGCTCA GATAACACAG 
ACACACAACA GCTTTGACTC ACGACGGAGC AGCATTTCCT CTGGGATTTC TTCAGCTTCT 
TGGGCTAACA ACCAGGCTAG ACGGGGTCGC CTCCTCTATG CGCCCCTCAT TTTGGTGAAC 
GAGGTGGGGG GCACTCAGGC AATGTGGAGT GACACCAATG GCAGCACCAC TCTTCCAGCA 
TCAGCACGTC CGGGGTGGCT TGGTAGTGCA ATGGCAGGTG TGCCCCAGCA GCCTCGAGCA 
TACACTACTC TCAGGGTGCC ATCGCAAAAC AGCCCTGGAT ATATAGAGAG AGGAAGCGCT 
GACAGTCTTG TGGAATCGAT TCTGATTTCT GAAGGCTTGG GTCTATATGC CAAAGACCGC 
AAATTTGTTG ACTTCGCAAA ACGGGAGATT GCAGATGCCT GTCAAATGAC TGTTGATGAA 
ATGGAGAACG CCGCAACTGA CCTTTTATGT AGAGGAAGGG CAGGGCAACC AACCGGACGC 
TTCGATGAGG ATCTGTCTGA TGAGATGAAC TGTGTGATCT CGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334158.1
CDS	367..6714
Translation	MEAATKDGVGLDPSKTDTLNSTGSAGQKRAGGAKKHTQANKSALRAPRALCCLTLSNPIRMAALALVEWKPFDIFILLAIFANCVALGVSKPFPEDDSNATNHNLEQVEYVFLVIFTIETFTKILAYGLVMHPSAYIRSGWNLLDFIIVIVGLFSVIAEMGGDGQKADSHHAAGKPGGLDVKALRAFRVLRPLRLVSGVPSLQIVLNSIMKAMVPLLHIGLLVMFVIIIYAIIGLELFIGRMHKTCFYVGTELMVEDDPTPCAFAGHGRVCIGNNTDCRGGWEGPNGGITNFDNIFFAMLTVFQCITMEGWTDVLYWMNDSIGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAVCRGELQKAQEKQQMEEDMIGYMDWLIEAEDMDEDGNKRAVVAKKKMMKKYGWYKHSEDGESDSDSEFEAFLDDDNGCCASIMARLMAISFCEQLYHLNVVMRKNCRVAVKSTNFYWLVLLLVFLNTAASASEHYGQPKWLTDIQERANKILLALFTLEMLMKIYSFGFQIYFMALFNRFDCFVVCGGILETVLVEMEVIPPIGISVLRCVRLLRIFKVTRHWAALSDLVGSLLNSMKAICSLLLLLFLFLIIFALLGMQLFGGKFNFDETQMKRSTFDSFPSALLTCFQILTGEDWNSVMYDGIMAYGGPVFPNMIVCIYFVILFVCGNYILLNVFLAIAVDNLAGDGGKKKKEEKKEEEEEWEDEDEREEDDAGNEMDDWEENEELRAIEGLEGIGTPMKVESFQPKEKIVPIPDGSSFFVLGKKNCLRVACHNLIHHHYFTNFILIFIILSSFSLAAEDPIKTHSVRNVMLGYADYVFTTVFTIEIMLKMTVYGAFLHQGSFCRNAFNLLDLLVVSVSLTSFFLNSSAISVVKILRVLRVLRPLRAINRAKGLKSVIQCVFVAIRTIGNILIVTTLLQFMFACIGVQLFKGRFYRCTDEAKHTPEECKGTFVVFKDGDMNHPMVRERVWENSEFNFDNVLMGMLALFTVSTFEGWPQLLYKAIDANAENRGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIITFREQGEAEFKNCELNKNQRQCVYYALKAQPIKIYIPKNPSQLKFWKIINSSQFEYIMFVLILLNTLTLAVQHYEQSKLFNSVMDILNMIFTTLFTVEMIIKLMALRPYHYFIDAWNSFDALIVVGSLVDIMIAELSGGGGHGEGKEGESAKVSITFFRLFRVMRLVKLLSKGEGIRTLLWTFVKSLQALPYVGLLIAMIFFIYGVIGMQIFGKIAIDDDTELNRNNNFQTFFMAVLLLFRCATGEQWQQIMLAALPGHRCDPESDFEAGEEFTCGSNLAYLYFISFFALCAFLIINLFIAVIMDNFEYLTRDWTVLGTHHLDEFKRVWSDYDPEATGRIKHLDVVTMLRRIQPPLGFGKLCPHRVACRRLVAMNVPLYPDGTVSFNATLFALVRTSLKIKTDGPIDQQNEELRAIIKKIWKRTKPKLLDEVIPPPSGNEVTAGKFYASFLIQDYFKKFRKRKEKERKKKGKDKSASLQAGLRTLQDLAPEMRLAMAMEDEEALEGEMMEDEEFFRSDSVFSDVPPTPTMSTPRSTPMPPHLDQTTVNIELPPRIRNGSLMLEEALLDSRPASALSENEVIVEQPNRLSPLPSKWTSSHSKLTPLWILNTNPRQGAQNDSTGVCVYPPSPSPDVAMQNGQVTEGDVVPGGHGGATMGGVMEAGVVHGVATDGVVHGGSIIGEEVVMGGGVTELTYTADQGGVGSGDPQTAVLPEQQWFPEVPPTQAVVPDRTTECVAPVQPINLNGNGYQGNIQDAGNYNGSFPGNGYNGNGTNGTDYPGNRCSSNGYSTNGYNGNGYNSNGFNGNGYNNNEYSGNGFAARRRMLPPTPLGRKPNFNIQCLRRQGSNEDLPVPGTYHQNSPPCRARAQITQTHNSFDSRRSSISSGISSASWANNQARRGRLLYAPLILVNEVGGTQAMWSDTNGSTTLPASARPGWLGSAMAGVPQQPRAYTTLRVPSQNSPGYIERGSADSLVESILISEGLGLYAKDRKFVDFAKREIADACQMTVDEMENAATDLLCRGRAGQPTGRFDEDLSDEMNCVISY

Target ORF information:

RefSeq Version	XM_021478483.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, L type, alpha 1F subunit a (cacna1fa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478483.1

ORF Insert Sequence:

ATGGAAGCGG CAACGAAAGA TGGGGTTGGC TTGGACCCCA GCAAAACGGA CACACTCAAC 
TCAACAGGTT CTGCAGGACA AAAAAGAGCT GGAGGAGCCA AGAAGCACAC CCAAGCTAAC 
AAATCAGCCT TGCGTGCACC CAGAGCTCTG TGCTGCCTTA CTCTCAGCAA TCCTATTCGC 
ATGGCAGCTC TGGCTCTAGT GGAGTGGAAG CCATTTGATA TATTCATTTT ACTGGCCATC 
TTCGCAAATT GTGTTGCTCT TGGTGTGTCC AAACCGTTTC CTGAGGATGA TTCCAATGCC 
ACCAACCACA ATCTGGAACA AGTGGAGTAT GTGTTTCTGG TCATTTTCAC CATCGAAACT 
TTCACAAAGA TTCTGGCATA TGGTCTGGTC ATGCATCCCA GTGCCTACAT CCGCAGCGGG 
TGGAACTTGC TGGATTTTAT CATTGTCATT GTTGGTCTTT TCAGTGTGAT AGCTGAAATG 
GGAGGAGACG GTCAAAAAGC AGACAGCCAT CATGCAGCCG GTAAACCAGG TGGTCTGGAT 
GTCAAAGCCC TGAGAGCTTT TAGAGTTTTG CGACCACTTC GTCTAGTGTC TGGTGTGCCG 
AGTCTGCAGA TTGTGTTAAA CTCGATCATG AAGGCCATGG TGCCTCTGCT CCATATTGGT 
CTGCTCGTCA TGTTTGTCAT CATCATATAT GCAATCATTG GTCTGGAGCT TTTTATAGGC 
AGGATGCACA AGACCTGCTT TTATGTAGGG ACAGAACTTA TGGTGGAGGA TGATCCAACA 
CCATGTGCTT TTGCCGGTCA CGGACGCGTC TGTATAGGCA ATAACACAGA TTGTCGTGGA 
GGATGGGAGG GACCAAATGG AGGCATCACC AACTTTGATA ATATCTTCTT TGCCATGCTC 
ACTGTGTTCC AGTGTATTAC AATGGAGGGC TGGACTGATG TCCTTTACTG GATGAACGAT 
TCTATTGGGT TTGAGTTGCC TTGGGTTTAT TTTGTGTCTC TGGTCATTTT TGGTTCATTC 
TTTGTCCTGA ATCTTGTATT GGGTGTGCTG AGCGGCGAGT TCTCTAAAGA GAGAGAGAAG 
GCAGTGTGTC GCGGTGAGCT GCAGAAGGCT CAGGAGAAAC AGCAGATGGA GGAGGACATG 
ATCGGCTACA TGGACTGGCT CATTGAAGCT GAGGATATGG ATGAAGATGG AAATAAGCGT 
GCTGTGGTGG CCAAGAAAAA GATGATGAAG AAATATGGCT GGTACAAGCA TAGTGAAGAT 
GGAGAATCTG ATTCTGATTC GGAGTTTGAA GCATTCCTGG ATGATGACAA TGGCTGCTGT 
GCTTCCATAA TGGCACGACT GATGGCTATA AGTTTCTGTG AGCAACTGTA CCACTTGAAT 
GTAGTTATGA GGAAGAACTG TCGGGTTGCA GTCAAATCCA CCAATTTCTA CTGGCTGGTG 
CTACTACTAG TGTTCCTCAA CACTGCTGCC AGCGCTTCTG AACATTATGG CCAGCCCAAG 
TGGCTCACTG ACATACAGGA ACGAGCAAAT AAGATCCTTC TGGCTCTGTT TACGCTGGAG 
ATGCTGATGA AGATTTACAG CTTCGGGTTT CAGATCTACT TCATGGCACT CTTCAATCGT 
TTTGATTGTT TCGTGGTGTG TGGTGGAATT CTGGAAACTG TGCTTGTGGA GATGGAGGTG 
ATCCCACCCA TTGGAATCTC TGTGCTGCGC TGTGTCCGTC TGCTCCGTAT CTTCAAAGTC 
ACAAGACACT GGGCTGCCCT CTCAGACCTG GTGGGCTCTT TGCTGAACTC TATGAAGGCC 
ATTTGCTCCC TGCTGTTGCT GCTATTCCTC TTCCTCATTA TTTTTGCTCT CCTGGGAATG 
CAGCTTTTCG GTGGAAAGTT CAACTTTGAT GAGACTCAAA TGAAAAGAAG CACCTTCGAC 
TCTTTTCCAT CTGCCTTACT CACCTGTTTC CAGATCCTAA CAGGAGAGGA CTGGAACTCC 
GTCATGTATG ATGGCATCAT GGCTTATGGT GGACCAGTGT TTCCCAATAT GATTGTCTGC 
ATTTACTTTG TCATTCTTTT CGTCTGTGGT AACTACATCT TACTGAACGT CTTCTTGGCT 
ATTGCTGTGG ATAACTTGGC TGGAGATGGT GGCAAAAAGA AAAAAGAAGA GAAAAAGGAA 
GAGGAGGAGG AATGGGAAGA TGAAGATGAA AGAGAGGAAG ATGATGCTGG GAATGAGATG 
GATGATTGGG AAGAGAATGA AGAGCTGAGA GCAATCGAAG GGCTTGAGGG TATAGGCACT 
CCAATGAAGG TCGAAAGTTT CCAACCCAAA GAGAAAATTG TGCCTATTCC TGATGGCAGT 
TCCTTTTTTG TCCTTGGAAA GAAAAACTGT CTCAGAGTCG CCTGCCACAA CCTCATTCAT 
CATCATTACT TCACCAACTT CATCCTCATC TTCATTATAC TTAGTAGTTT TTCCTTGGCT 
GCTGAAGACC CAATCAAAAC ACATTCTGTC AGGAATGTTA TGCTGGGCTA TGCTGACTAC 
GTCTTTACCA CAGTTTTCAC TATTGAGATC ATGTTGAAGA TGACTGTGTA TGGAGCTTTC 
CTTCATCAAG GTTCCTTCTG CAGAAATGCC TTCAACCTTC TGGACCTTCT TGTCGTCAGT 
GTGTCCCTCA CCTCTTTCTT TTTGAATTCG AGTGCCATTT CTGTGGTGAA GATTCTTAGA 
GTTTTGCGAG TGCTTAGGCC TCTTCGAGCC ATCAACAGGG CAAAGGGGCT CAAGAGCGTG 
ATTCAGTGTG TATTTGTGGC CATCCGCACT ATTGGCAACA TCCTCATTGT CACCACTCTT 
CTCCAGTTCA TGTTCGCTTG TATTGGTGTC CAGCTTTTTA AGGGCCGGTT CTATAGATGC 
ACAGATGAAG CGAAGCATAC ACCTGAAGAG TGCAAGGGCA CTTTTGTTGT GTTCAAAGAT 
GGTGATATGA ACCATCCAAT GGTGAGGGAG AGAGTTTGGG AAAACAGCGA GTTTAACTTT 
GACAATGTTC TGATGGGCAT GCTTGCGCTC TTCACTGTCT CTACATTTGA GGGCTGGCCA 
CAGCTCTTGT ACAAAGCCAT TGATGCCAAT GCAGAGAACA GAGGACCCAT CTATAACTAC 
CGTGTAGAGA TTTCCATCTT CTTCATCATC TACATTATCA TCATTGCCTT CTTCATGATG 
AACATCTTTG TGGGTTTTGT CATTATCACA TTTCGTGAAC AAGGAGAGGC AGAGTTTAAA 
AACTGTGAAC TAAATAAAAA TCAGCGTCAG TGCGTGTATT ATGCACTGAA AGCTCAACCA 
ATAAAGATTT ACATTCCCAA AAACCCTTCC CAACTTAAAT TTTGGAAGAT CATAAATTCC 
TCTCAGTTCG AGTATATCAT GTTTGTCCTC ATTCTGCTCA ACACACTCAC GTTGGCTGTA 
CAGCATTATG AGCAATCCAA ACTCTTCAAC TCTGTGATGG ACATACTGAA CATGATCTTC 
ACGACGCTCT TTACTGTTGA GATGATCATC AAACTCATGG CTCTGAGACC TTATCATTAT 
TTTATAGATG CCTGGAATTC TTTTGATGCA CTGATAGTGG TGGGCAGTTT GGTGGACATC 
ATGATTGCAG AGCTGAGCGG TGGTGGAGGC CATGGTGAGG GAAAAGAAGG CGAAAGTGCC 
AAAGTATCCA TCACTTTTTT CCGTCTTTTC CGTGTTATGC GACTGGTCAA GCTGCTCAGC 
AAGGGTGAAG GCATCCGTAC ACTCCTTTGG ACTTTCGTTA AATCACTACA GGCTTTACCA 
TACGTTGGCC TACTCATTGC GATGATCTTT TTTATTTATG GTGTGATTGG CATGCAGATA 
TTTGGGAAGA TAGCTATAGA CGACGATACA GAGCTTAATC GGAACAACAA TTTCCAAACC 
TTTTTCATGG CTGTGCTTTT GCTGTTCAGG TGTGCAACAG GTGAACAGTG GCAGCAGATC 
ATGCTTGCAG CACTGCCGGG GCACCGGTGT GACCCAGAGT CTGATTTTGA GGCGGGGGAG 
GAGTTTACCT GCGGAAGCAA CCTGGCATAC CTCTATTTCA TCAGTTTCTT TGCACTCTGT 
GCCTTTTTGA TTATTAACTT GTTCATTGCT GTCATCATGG ATAACTTTGA GTATCTGACT 
AGAGACTGGA CTGTGCTTGG CACTCATCAC CTCGATGAGT TTAAACGAGT CTGGTCAGAT 
TACGATCCAG AGGCCACGGG ACGGATTAAA CATTTAGATG TAGTTACCAT GCTGCGCAGA 
ATTCAGCCTC CACTGGGGTT TGGAAAACTC TGTCCACATC GAGTGGCCTG TAGGAGACTG 
GTTGCTATGA ATGTTCCTCT CTATCCTGAT GGCACTGTGT CCTTCAACGC CACCCTATTT 
GCTCTTGTCA GAACTTCACT CAAGATTAAA ACAGATGGCC CCATTGATCA ACAGAATGAA 
GAGCTGAGAG CTATTATTAA AAAGATCTGG AAACGCACCA AACCCAAACT CCTAGATGAA 
GTTATACCTC CCCCTTCAGG CAATGAAGTG ACTGCAGGGA AGTTTTATGC CAGCTTTCTA 
ATTCAGGACT ACTTTAAAAA GTTTCGCAAG AGAAAGGAGA AAGAGAGGAA GAAGAAAGGC 
AAAGACAAAT CAGCCTCTCT CCAGGCTGGG TTACGCACAT TACAGGATCT TGCCCCAGAG 
ATGCGACTAG CTATGGCGAT GGAGGATGAG GAAGCACTGG AGGGGGAGAT GATGGAAGAT 
GAGGAGTTCT TTAGGAGTGA CAGCGTCTTC AGTGATGTTC CTCCTACACC AACTATGTCC 
ACCCCTAGAA GCACGCCTAT GCCCCCACAT CTGGATCAGA CCACAGTAAA CATCGAGCTT 
CCCCCAAGAA TACGCAATGG AAGCTTAATG CTGGAGGAAG CCCTGTTGGA CTCCCGTCCA 
GCCTCTGCCC TATCTGAAAA TGAAGTGATA GTTGAACAGC CTAACAGGTT GTCTCCCTTA 
CCCAGCAAGT GGACCTCCAG TCACAGTAAA CTTACCCCAT TGTGGATACT GAATACCAAC 
CCAAGACAAG GAGCACAGAA TGACAGCACT GGAGTTTGTG TCTATCCACC TTCCCCATCT 
CCGGATGTAG CCATGCAAAA TGGACAAGTC ACAGAAGGGG ATGTCGTTCC AGGAGGACAT 
GGAGGAGCAA CTATGGGAGG AGTCATGGAA GCGGGAGTTG TCCATGGGGT AGCAACAGAT 
GGAGTAGTCC ATGGGGGATC AATCATAGGA GAAGAAGTAG TCATGGGAGG AGGAGTCACA 
GAATTGACAT ACACTGCAGA CCAAGGTGGT GTGGGCAGTG GGGACCCTCA GACTGCAGTC 
TTACCTGAAC AACAGTGGTT TCCTGAAGTT CCGCCAACAC AAGCTGTAGT ACCTGATAGG 
ACCACTGAAT GTGTGGCTCC AGTTCAGCCC ATCAACCTTA ACGGCAATGG TTACCAAGGA 
AACATCCAAG ATGCTGGCAA CTACAATGGC AGTTTCCCTG GAAATGGCTA CAATGGGAAT 
GGTACCAATG GAACTGATTA TCCAGGCAAT CGTTGCTCCA GTAACGGTTA TAGCACCAAT 
GGCTACAATG GTAATGGTTA CAATAGTAAT GGATTCAATG GTAATGGATA CAACAATAAT 
GAATACAGTG GCAATGGTTT TGCTGCACGG CGAAGAATGC TTCCCCCAAC ACCATTAGGC 
CGGAAGCCAA ACTTTAACAT TCAGTGTTTG CGAAGGCAGG GCAGTAATGA GGATCTCCCA 
GTCCCTGGCA CATATCATCA AAACTCCCCA CCCTGCAGGG CTCGCGCTCA GATAACACAG 
ACACACAACA GCTTTGACTC ACGACGGAGC AGCATTTCCT CTGGGATTTC TTCAGCTTCT 
TGGGCTAACA ACCAGGCTAG ACGGGGTCGC CTCCTCTATG CGCCCCTCAT TTTGGTGAAC 
GAGGTGGGGG GCACTCAGGC AATGTGGAGT GACACCAATG GCAGCACCAC TCTTCCAGCA 
TCAGCACGTC CGGGGTGGCT TGGTAGTGCA ATGGCAGGTG TGCCCCAGCA GCCTCGAGCA 
TACACTACTC TCAGGGTGCC ATCGCAAAAC AGCCCTGGAT ATATAGAGAG AGGAAGCGCT 
GACAGTCTTG TGGAATCGAT TCTGATTTCT GAAGGCTTGG GTCTATATGC CAAAGACCGC 
AAATTTGTTG ACTTCGCAAA ACGGGAGATT GCAGATGCCT GTCAAATGAC TGTTGATGAA 
ATGGAGAACG CCGCAACTGA CCTTTTATGT AGAGGAAGGG CAGGGCAACC AACCGGACGC 
TTCGATGAGG ATCTGTCTGA TGAGATGAAC TGTGTGATCT CGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1fa ( XM_017357598.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213087.1 Danio rerio calcium channel, voltage-dependent, L type, alpha 1F subunit a (cacna1fa), mRNA.

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CloneID	ODa60181
Clone ID Related Accession (Same CDS sequence)	XM_017357598.1
Accession Version	XM_017357598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent L-type calcium channel subunit alpha-1D
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTGTACCC TTAAGCCATT TGATATATTC ATTTTACTGG CCATCTTCGC AAATTGTGTT 
GCTCTTGGTG TGTCCAAACC GTTTCCTGAG GATGATTCCA ATGCCACCAA CCACAATCTG 
GAACAAGTGG AGTATGTGTT TCTGGTCATT TTCACCATCG AAACTTTCAC AAAGATTCTG 
GCATATGGTC TGGTCATGCA TCCCAGTGCC TACATCCGCA GCGGGTGGAA CTTGCTGGAT 
TTTATCATTG TCATTGTTGG TCTTTTCAGT GTGATAGCTG AAATGGGAGG AGACGGTCAA 
AAAGCAGACA GCCATCATGC AGCCGGTAAA CCAGGTGGTC TGGATGTCAA AGCCCTGAGA 
GCTTTTAGAG TTTTGCGACC ACTTCGTCTA GTGTCTGGTG TGCCGAGTCT GCAGATTGTG 
TTAAACTCGA TCATGAAGGC CATGGTGCCT CTGCTCCATA TTGGTCTGCT CGTCATGTTT 
GTCATCATCA TATATGCAAT CATTGGTCTG GAGCTTTTTA TAGGCAGGAT GCACAAGACC 
TGCTTTTATG TAGGGACAGA ACTTATGGTG GAGGATGATC CAACACCATG TGCTTTTGCC 
GGTCACGGAC GCGTCTGTAT AGGCAATAAC ACAGATTGTC GTGGAGGATG GGAGGGACCA 
AATGGAGGCA TCACCAACTT TGATAATATC TTCTTTGCCA TGCTCACTGT GTTCCAGTGT 
ATTACAATGG AGGGCTGGAC TGATGTCCTT TACTGGATGA ACGATTCTAT TGGGTTTGAG 
TTGCCTTGGG TTTATTTTGT GTCTCTGGTC ATTTTTGGTT CATTCTTTGT CCTGAATCTT 
GTATTGGGTG TGCTGAGCGG CGAGTTCTCT AAAGAGAGAG AGAAGGCAGT GTGTCGCGGT 
GAGCTGCAGA AGGCTCAGGA GAAACAGCAG ATGGAGGAGG ACATGATCGG CTACATGGAC 
TGGCTCATTG AAGCTGAGGA TATGGATGAA GATGGAAATA AGCGTGCTGT GGTGGCCAAG 
AAAAAGATGA TGAAGAAATA TGGCTGGTAC AAGCATAGTG AAGATGGAGA ATCTGATTCT 
GATTCGGAGT TTGAAGCATT CCTGGATGAT GACAATGGCT GCTGTGCTTC CATAATGGCA 
CGACTGATGG CTATAAGTTT CTGTGAGCAA CTGTACCACT TGAATGTAGT TATGAGGAAG 
AACTGTCGGG TTGCAGTCAA ATCCACCAAT TTCTACTGGC TGGTGCTACT ACTAGTGTTC 
CTCAACACTG CTGCCAGCGC TTCTGAACAT TATGGCCAGC CCAAGTGGCT CACTGACATA 
CAGGAACGAG CAAATAAGAT CCTTCTGGCT CTGTTTACGC TGGAGATGCT GATGAAGATT 
TACAGCTTCG GGTTTCAGAT CTACTTCATG GCACTCTTCA ATCGTTTTGA TTGTTTCGTG 
GTGTGTGGTG GAATTCTGGA AACTGTGCTT GTGGAGATGG AGGTGATCCC ACCCATTGGA 
ATCTCTGTGC TGCGCTGTGT CCGTCTGCTC CGTATCTTCA AAGTCACAAG ACACTGGGCT 
GCCCTCTCAG ACCTGGTGGG CTCTTTGCTG AACTCTATGA AGGCCATTTG CTCCCTGCTG 
TTGCTGCTAT TCCTCTTCCT CATTATTTTT GCTCTCCTGG GAATGCAGCT TTTCGGTGGA 
AAGTTCAACT TTGATGAGAC TCAAATGAAA AGAAGCACCT TCGACTCTTT TCCATCTGCC 
TTACTCACCT GTTTCCAGAT CCTAACAGGA GAGGACTGGA ACTCCGTCAT GTATGATGGC 
ATCATGGCTT ATGGTGGACC AGTGTTTCCC AATATGATTG TCTGCATTTA CTTTGTCATT 
CTTTTCGTCT GTGGTAACTA CATCTTACTG AACGTCTTCT TGGCTATTGC TGTGGATAAC 
TTGGCTGGAG ATGGTGGCAA AAAGAAAAAA GAAGAGAAAA AGGAAGAGGA GGAGGAATGG 
GAAGATGAAG ATGAAAGAGA GGAAGATGAT GCTGGGAATG AGATGGATGA TTGGGAAGAG 
AATGAAGAGC TGAGAGCAAT CGAAGGGCTT GAGGGTATAG GCACTCCAAT GAAGGTCGAA 
AGTTTCCAAC CCAAAGAGAA AATTGTGCCT ATTCCTGATG GCAGTTCCTT TTTTGTCCTT 
GGAAAGAAAA ACTGTCTCAG AGTCGCCTGC CACAACCTCA TTCATCATCA TTACTTCACC 
AACTTCATCC TCATCTTCAT TATACTTAGT AGTTTTTCCT TGGCTGCTGA AGACCCAATC 
AAAACACATT CTGTCAGGAA TGTTATGCTG GGCTATGCTG ACTACGTCTT TACCACAGTT 
TTCACTATTG AGATCATGTT GAAGATGACT GTGTATGGAG CTTTCCTTCA TCAAGGTTCC 
TTCTGCAGAA ATGCCTTCAA CCTTCTGGAC CTTCTTGTCG TCAGTGTGTC CCTCACCTCT 
TTCTTTTTGA ATTCGAGTGC CATTTCTGTG GTGAAGATTC TTAGAGTTTT GCGAGTGCTT 
AGGCCTCTTC GAGCCATCAA CAGGGCAAAG GGGCTCAAGA GCGTGATTCA GTGTGTATTT 
GTGGCCATCC GCACTATTGG CAACATCCTC ATTGTCACCA CTCTTCTCCA GTTCATGTTC 
GCTTGTATTG GTGTCCAGCT TTTTAAGGGC CGGTTCTATA GATGCACAGA TGAAGCGAAG 
CATACACCTG AAGAGTGCAA GGGCACTTTT GTTGTGTTCA AAGATGGTGA TATGAACCAT 
CCAATGGTGA GGGAGAGAGT TTGGGAAAAC AGCGAGTTTA ACTTTGACAA TGTTCTGATG 
GGCATGCTTG CGCTCTTCAC TGTCTCTACA TTTGAGGGCT GGCCACAGCT CTTGTACAAA 
GCCATTGATG CCAATGCAGA GAACAGAGGA CCCATCTATA ACTACCGTGT AGAGATTTCC 
ATCTTCTTCA TCATCTACAT TATCATCATT GCCTTCTTCA TGATGAACAT CTTTGTGGGT 
TTTGTCATTA TCACATTTCG TGAACAAGGA GAGGCAGAGT TTAAAAACTG TGAACTAAAT 
AAAAATCAGC GTCAGTGCGT GTATTATGCA CTGAAAGCTC AACCAATAAA GATTTACATT 
CCCAAAAACC CTTCCCAACT TAAATTTTGG AAGATCATAA ATTCCTCTCA GTTCGAGTAT 
ATCATGTTTG TCCTCATTCT GCTCAACACA CTCACGTTGG CTGTACAGCA TTATGAGCAA 
TCCAAACTCT TCAACTCTGT GATGGACATA CTGAACATGA TCTTCACGAC GCTCTTTACT 
GTTGAGATGA TCATCAAACT CATGGCTCTG AGACCTTATC ATTATTTTAT AGATGCCTGG 
AATTCTTTTG ATGCACTGAT AGTGGTGGGC AGTTTGGTGG ACATCATGAT TGCAGAGCTG 
AGCGGTGGTG GAGGCCATGG TGAGGGAAAA GAAGGCGAAA GTGCCAAAGT ATCCATCACT 
TTTTTCCGTC TTTTCCGTGT TATGCGACTG GTCAAGCTGC TCAGCAAGGG TGAAGGCATC 
CGTACACTCC TTTGGACTTT CGTTAAATCA CTACAGGCTT TACCATACGT TGGCCTACTC 
ATTGCGATGA TCTTTTTTAT TTATGGTGTG ATTGGCATGC AGATATTTGG GAAGATAGCT 
ATAGACGACG ATACAGAGCT TAATCGGAAC AACAATTTCC AAACCTTTTT CATGGCTGTG 
CTTTTGCTGT TCAGGTGTGC AACAGGTGAA CAGTGGCAGC AGATCATGCT TGCAGCACTG 
CCGGGGCACC GGTGTGACCC AGAGTCTGAT TTTGAGGCGG GGGAGGAGTT TACCTGCGGA 
AGCAACCTGG CATACCTCTA TTTCATCAGT TTCTTTGCAC TCTGTGCCTT TTTGATTATT 
AACTTGTTCA TTGCTGTCAT CATGGATAAC TTTGAGTATC TGACTAGAGA CTGGACTGTG 
CTTGGCACTC ATCACCTCGA TGAGTTTAAA CGAGTCTGGT CAGATTACGA TCCAGAGGCC 
ACGGGACGGA TTAAACATTT AGATGTAGTT ACCATGCTGC GCAGAATTCA GCCTCCACTG 
GGGTTTGGAA AACTCTGTCC ACATCGAGTG GCCTGTAGGA GACTGGTTGC TATGAATGTT 
CCTCTCTATC CTGATGGCAC TGTGTCCTTC AACGCCACCC TATTTGCTCT TGTCAGAACT 
TCACTCAAGA TTAAAACAGA TGGCCCCATT GATCAACAGA ATGAAGAGCT GAGAGCTATT 
ATTAAAAAGA TCTGGAAACG CACCAAACCC AAACTCCTAG ATGAAGTTAT ACCTCCCCCT 
TCAGGCAATG AAGTGACTGC AGGGAAGTTT TATGCCAGCT TTCTAATTCA GGACTACTTT 
AAAAAGTTTC GCAAGAGAAA GGAGAAAGAG AGGAAGAAGA AAGGCAAAGA CAAATCAGCC 
TCTCTCCAGG CTGGGTTACG CACATTACAG GATCTTGCCC CAGAGATGCG ACTAGCTATG 
GCGATGGAGG ATGAGGAAGC ACTGGAGGGG GAGATGATGG AAGATGAGGA GTTCTTTAGG 
AGTGACAGCG TCTTCAGTGA TGTTCCTCCT ACACCAACTA TGTCCACCCC TAGAAGCACG 
CCTATGCCCC CACATCTGGA TCAGACCACA GTAAACATCG AGCTTCCCCC AAGAATACGC 
AATGGAAGCT TAATGCTGGA GGAAGCCCTG TTGGACTCCC GTCCAGCCTC TGCCCTATCT 
GAAAATGAAG TGATAGTTGA ACAGCCTAAC AGGTTGTCTC CCTTACCCAG CAAGTGGACC 
TCCAGTCACA GTAAACTTAC CCCATTGTGG ATACTGAATA CCAACCCAAG ACAAGGAGCA 
CAGAATGACA GCACTGGAGT TTGTGTCTAT CCACCTTCCC CATCTCCGGA TGTAGCCATG 
CAAAATGGAC AAGTCACAGA AGGGGATGTC GTTCCAGGAG GACATGGAGG AGCAACTATG 
GGAGGAGTCA TGGAAGCGGG AGTTGTCCAT GGGGTAGCAA CAGATGGAGT AGTCCATGGG 
GGATCAATCA TAGGAGAAGA AGTAGTCATG GGAGGAGGAG TCACAGAATT GACATACACT 
GCAGACCAAG GTGGTGTGGG CAGTGGGGAC CCTCAGACTG CAGTCTTACC TGAACAACAG 
TGGTTTCCTG AAGTTCCGCC AACACAAGCT GTAGTACCTG ATAGGACCAC TGAATGTGTG 
GCTCCAGTTC AGCCCATCAA CCTTAACGGC AATGGTTACC AAGGAAACAT CCAAGATGCT 
GGCAACTACA ATGGCAGTTT CCCTGGAAAT GGCTACAATG GGAATGGTAC CAATGGAACT 
GATTATCCAG GCAATCGTTG CTCCAGTAAC GGTTATAGCA CCAATGGCTA CAATGGTAAT 
GGTTACAATA GTAATGGATT CAATGGTAAT GGATACAACA ATAATGAATA CAGTGGCAAT 
GGTTTTGCTG CACGGCGAAG AATGCTTCCC CCAACACCAT TAGGCCGGAA GCCAAACTTT 
AACATTCAGT GTTTGCGAAG GCAGGGCAGT AATGAGGATC TCCCAGTCCC TGGCACATAT 
CATCAAAACT CCCCACCCTG CAGGGCTCGC GCTCAGATAA CACAGACACA CAACAGCTTT 
GACTCACGAC GGAGCAGCAT TTCCTCTGGG ATTTCTTCAG CTTCTTGGGC TAACAACCAG 
GCTAGACGGG GTCGCCTCCT CTATGCGCCC CTCATTTTGG TGAACGAGGT GGGGGGCACT 
CAGGCAATGT GGAGTGACAC CAATGGCAGC ACCACTCTTC CAGCATCAGC ACGTCCGGGG 
TGGCTTGGTA GTGCAATGGC AGGTGTGCCC CAGCAGCCTC GAGCATACAC TACTCTCAGG 
GTGCCATCGC AAAACAGCCC TGGATATATA GAGAGAGGAA GCGCTGACAG TCTTGTGGAA 
TCGATTCTGA TTTCTGAAGG CTTGGGTCTA TATGCCAAAG ACCGCAAATT TGTTGACTTC 
GCAAAACGGG AGATTGCAGA TGCCTGTCAA ATGACTGTTG ATGAAATGGA GAACGCCGCA 
ACTGACCTTT TATGTAGAGG AAGGGCAGGG CAACCAACCG GACGCTTCGA TGAGGATCTG 
TCTGATGAGA TGAACTGTGT GATCTCGTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213087.1
CDS	1..6153
Translation	MCTLKPFDIFILLAIFANCVALGVSKPFPEDDSNATNHNLEQVEYVFLVIFTIETFTKILAYGLVMHPSAYIRSGWNLLDFIIVIVGLFSVIAEMGGDGQKADSHHAAGKPGGLDVKALRAFRVLRPLRLVSGVPSLQIVLNSIMKAMVPLLHIGLLVMFVIIIYAIIGLELFIGRMHKTCFYVGTELMVEDDPTPCAFAGHGRVCIGNNTDCRGGWEGPNGGITNFDNIFFAMLTVFQCITMEGWTDVLYWMNDSIGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAVCRGELQKAQEKQQMEEDMIGYMDWLIEAEDMDEDGNKRAVVAKKKMMKKYGWYKHSEDGESDSDSEFEAFLDDDNGCCASIMARLMAISFCEQLYHLNVVMRKNCRVAVKSTNFYWLVLLLVFLNTAASASEHYGQPKWLTDIQERANKILLALFTLEMLMKIYSFGFQIYFMALFNRFDCFVVCGGILETVLVEMEVIPPIGISVLRCVRLLRIFKVTRHWAALSDLVGSLLNSMKAICSLLLLLFLFLIIFALLGMQLFGGKFNFDETQMKRSTFDSFPSALLTCFQILTGEDWNSVMYDGIMAYGGPVFPNMIVCIYFVILFVCGNYILLNVFLAIAVDNLAGDGGKKKKEEKKEEEEEWEDEDEREEDDAGNEMDDWEENEELRAIEGLEGIGTPMKVESFQPKEKIVPIPDGSSFFVLGKKNCLRVACHNLIHHHYFTNFILIFIILSSFSLAAEDPIKTHSVRNVMLGYADYVFTTVFTIEIMLKMTVYGAFLHQGSFCRNAFNLLDLLVVSVSLTSFFLNSSAISVVKILRVLRVLRPLRAINRAKGLKSVIQCVFVAIRTIGNILIVTTLLQFMFACIGVQLFKGRFYRCTDEAKHTPEECKGTFVVFKDGDMNHPMVRERVWENSEFNFDNVLMGMLALFTVSTFEGWPQLLYKAIDANAENRGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIITFREQGEAEFKNCELNKNQRQCVYYALKAQPIKIYIPKNPSQLKFWKIINSSQFEYIMFVLILLNTLTLAVQHYEQSKLFNSVMDILNMIFTTLFTVEMIIKLMALRPYHYFIDAWNSFDALIVVGSLVDIMIAELSGGGGHGEGKEGESAKVSITFFRLFRVMRLVKLLSKGEGIRTLLWTFVKSLQALPYVGLLIAMIFFIYGVIGMQIFGKIAIDDDTELNRNNNFQTFFMAVLLLFRCATGEQWQQIMLAALPGHRCDPESDFEAGEEFTCGSNLAYLYFISFFALCAFLIINLFIAVIMDNFEYLTRDWTVLGTHHLDEFKRVWSDYDPEATGRIKHLDVVTMLRRIQPPLGFGKLCPHRVACRRLVAMNVPLYPDGTVSFNATLFALVRTSLKIKTDGPIDQQNEELRAIIKKIWKRTKPKLLDEVIPPPSGNEVTAGKFYASFLIQDYFKKFRKRKEKERKKKGKDKSASLQAGLRTLQDLAPEMRLAMAMEDEEALEGEMMEDEEFFRSDSVFSDVPPTPTMSTPRSTPMPPHLDQTTVNIELPPRIRNGSLMLEEALLDSRPASALSENEVIVEQPNRLSPLPSKWTSSHSKLTPLWILNTNPRQGAQNDSTGVCVYPPSPSPDVAMQNGQVTEGDVVPGGHGGATMGGVMEAGVVHGVATDGVVHGGSIIGEEVVMGGGVTELTYTADQGGVGSGDPQTAVLPEQQWFPEVPPTQAVVPDRTTECVAPVQPINLNGNGYQGNIQDAGNYNGSFPGNGYNGNGTNGTDYPGNRCSSNGYSTNGYNGNGYNSNGFNGNGYNNNEYSGNGFAARRRMLPPTPLGRKPNFNIQCLRRQGSNEDLPVPGTYHQNSPPCRARAQITQTHNSFDSRRSSISSGISSASWANNQARRGRLLYAPLILVNEVGGTQAMWSDTNGSTTLPASARPGWLGSAMAGVPQQPRAYTTLRVPSQNSPGYIERGSADSLVESILISEGLGLYAKDRKFVDFAKREIADACQMTVDEMENAATDLLCRGRAGQPTGRFDEDLSDEMNCVISY

Target ORF information:

RefSeq Version	XM_017357598.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, L type, alpha 1F subunit a (cacna1fa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357598.1

ORF Insert Sequence:

ATGTGTACCC TTAAGCCATT TGATATATTC ATTTTACTGG CCATCTTCGC AAATTGTGTT 
GCTCTTGGTG TGTCCAAACC GTTTCCTGAG GATGATTCCA ATGCCACCAA CCACAATCTG 
GAACAAGTGG AGTATGTGTT TCTGGTCATT TTCACCATCG AAACTTTCAC AAAGATTCTG 
GCATATGGTC TGGTCATGCA TCCCAGTGCC TACATCCGCA GCGGGTGGAA CTTGCTGGAT 
TTTATCATTG TCATTGTTGG TCTTTTCAGT GTGATAGCTG AAATGGGAGG AGACGGTCAA 
AAAGCAGACA GCCATCATGC AGCCGGTAAA CCAGGTGGTC TGGATGTCAA AGCCCTGAGA 
GCTTTTAGAG TTTTGCGACC ACTTCGTCTA GTGTCTGGTG TGCCGAGTCT GCAGATTGTG 
TTAAACTCGA TCATGAAGGC CATGGTGCCT CTGCTCCATA TTGGTCTGCT CGTCATGTTT 
GTCATCATCA TATATGCAAT CATTGGTCTG GAGCTTTTTA TAGGCAGGAT GCACAAGACC 
TGCTTTTATG TAGGGACAGA ACTTATGGTG GAGGATGATC CAACACCATG TGCTTTTGCC 
GGTCACGGAC GCGTCTGTAT AGGCAATAAC ACAGATTGTC GTGGAGGATG GGAGGGACCA 
AATGGAGGCA TCACCAACTT TGATAATATC TTCTTTGCCA TGCTCACTGT GTTCCAGTGT 
ATTACAATGG AGGGCTGGAC TGATGTCCTT TACTGGATGA ACGATTCTAT TGGGTTTGAG 
TTGCCTTGGG TTTATTTTGT GTCTCTGGTC ATTTTTGGTT CATTCTTTGT CCTGAATCTT 
GTATTGGGTG TGCTGAGCGG CGAGTTCTCT AAAGAGAGAG AGAAGGCAGT GTGTCGCGGT 
GAGCTGCAGA AGGCTCAGGA GAAACAGCAG ATGGAGGAGG ACATGATCGG CTACATGGAC 
TGGCTCATTG AAGCTGAGGA TATGGATGAA GATGGAAATA AGCGTGCTGT GGTGGCCAAG 
AAAAAGATGA TGAAGAAATA TGGCTGGTAC AAGCATAGTG AAGATGGAGA ATCTGATTCT 
GATTCGGAGT TTGAAGCATT CCTGGATGAT GACAATGGCT GCTGTGCTTC CATAATGGCA 
CGACTGATGG CTATAAGTTT CTGTGAGCAA CTGTACCACT TGAATGTAGT TATGAGGAAG 
AACTGTCGGG TTGCAGTCAA ATCCACCAAT TTCTACTGGC TGGTGCTACT ACTAGTGTTC 
CTCAACACTG CTGCCAGCGC TTCTGAACAT TATGGCCAGC CCAAGTGGCT CACTGACATA 
CAGGAACGAG CAAATAAGAT CCTTCTGGCT CTGTTTACGC TGGAGATGCT GATGAAGATT 
TACAGCTTCG GGTTTCAGAT CTACTTCATG GCACTCTTCA ATCGTTTTGA TTGTTTCGTG 
GTGTGTGGTG GAATTCTGGA AACTGTGCTT GTGGAGATGG AGGTGATCCC ACCCATTGGA 
ATCTCTGTGC TGCGCTGTGT CCGTCTGCTC CGTATCTTCA AAGTCACAAG ACACTGGGCT 
GCCCTCTCAG ACCTGGTGGG CTCTTTGCTG AACTCTATGA AGGCCATTTG CTCCCTGCTG 
TTGCTGCTAT TCCTCTTCCT CATTATTTTT GCTCTCCTGG GAATGCAGCT TTTCGGTGGA 
AAGTTCAACT TTGATGAGAC TCAAATGAAA AGAAGCACCT TCGACTCTTT TCCATCTGCC 
TTACTCACCT GTTTCCAGAT CCTAACAGGA GAGGACTGGA ACTCCGTCAT GTATGATGGC 
ATCATGGCTT ATGGTGGACC AGTGTTTCCC AATATGATTG TCTGCATTTA CTTTGTCATT 
CTTTTCGTCT GTGGTAACTA CATCTTACTG AACGTCTTCT TGGCTATTGC TGTGGATAAC 
TTGGCTGGAG ATGGTGGCAA AAAGAAAAAA GAAGAGAAAA AGGAAGAGGA GGAGGAATGG 
GAAGATGAAG ATGAAAGAGA GGAAGATGAT GCTGGGAATG AGATGGATGA TTGGGAAGAG 
AATGAAGAGC TGAGAGCAAT CGAAGGGCTT GAGGGTATAG GCACTCCAAT GAAGGTCGAA 
AGTTTCCAAC CCAAAGAGAA AATTGTGCCT ATTCCTGATG GCAGTTCCTT TTTTGTCCTT 
GGAAAGAAAA ACTGTCTCAG AGTCGCCTGC CACAACCTCA TTCATCATCA TTACTTCACC 
AACTTCATCC TCATCTTCAT TATACTTAGT AGTTTTTCCT TGGCTGCTGA AGACCCAATC 
AAAACACATT CTGTCAGGAA TGTTATGCTG GGCTATGCTG ACTACGTCTT TACCACAGTT 
TTCACTATTG AGATCATGTT GAAGATGACT GTGTATGGAG CTTTCCTTCA TCAAGGTTCC 
TTCTGCAGAA ATGCCTTCAA CCTTCTGGAC CTTCTTGTCG TCAGTGTGTC CCTCACCTCT 
TTCTTTTTGA ATTCGAGTGC CATTTCTGTG GTGAAGATTC TTAGAGTTTT GCGAGTGCTT 
AGGCCTCTTC GAGCCATCAA CAGGGCAAAG GGGCTCAAGA GCGTGATTCA GTGTGTATTT 
GTGGCCATCC GCACTATTGG CAACATCCTC ATTGTCACCA CTCTTCTCCA GTTCATGTTC 
GCTTGTATTG GTGTCCAGCT TTTTAAGGGC CGGTTCTATA GATGCACAGA TGAAGCGAAG 
CATACACCTG AAGAGTGCAA GGGCACTTTT GTTGTGTTCA AAGATGGTGA TATGAACCAT 
CCAATGGTGA GGGAGAGAGT TTGGGAAAAC AGCGAGTTTA ACTTTGACAA TGTTCTGATG 
GGCATGCTTG CGCTCTTCAC TGTCTCTACA TTTGAGGGCT GGCCACAGCT CTTGTACAAA 
GCCATTGATG CCAATGCAGA GAACAGAGGA CCCATCTATA ACTACCGTGT AGAGATTTCC 
ATCTTCTTCA TCATCTACAT TATCATCATT GCCTTCTTCA TGATGAACAT CTTTGTGGGT 
TTTGTCATTA TCACATTTCG TGAACAAGGA GAGGCAGAGT TTAAAAACTG TGAACTAAAT 
AAAAATCAGC GTCAGTGCGT GTATTATGCA CTGAAAGCTC AACCAATAAA GATTTACATT 
CCCAAAAACC CTTCCCAACT TAAATTTTGG AAGATCATAA ATTCCTCTCA GTTCGAGTAT 
ATCATGTTTG TCCTCATTCT GCTCAACACA CTCACGTTGG CTGTACAGCA TTATGAGCAA 
TCCAAACTCT TCAACTCTGT GATGGACATA CTGAACATGA TCTTCACGAC GCTCTTTACT 
GTTGAGATGA TCATCAAACT CATGGCTCTG AGACCTTATC ATTATTTTAT AGATGCCTGG 
AATTCTTTTG ATGCACTGAT AGTGGTGGGC AGTTTGGTGG ACATCATGAT TGCAGAGCTG 
AGCGGTGGTG GAGGCCATGG TGAGGGAAAA GAAGGCGAAA GTGCCAAAGT ATCCATCACT 
TTTTTCCGTC TTTTCCGTGT TATGCGACTG GTCAAGCTGC TCAGCAAGGG TGAAGGCATC 
CGTACACTCC TTTGGACTTT CGTTAAATCA CTACAGGCTT TACCATACGT TGGCCTACTC 
ATTGCGATGA TCTTTTTTAT TTATGGTGTG ATTGGCATGC AGATATTTGG GAAGATAGCT 
ATAGACGACG ATACAGAGCT TAATCGGAAC AACAATTTCC AAACCTTTTT CATGGCTGTG 
CTTTTGCTGT TCAGGTGTGC AACAGGTGAA CAGTGGCAGC AGATCATGCT TGCAGCACTG 
CCGGGGCACC GGTGTGACCC AGAGTCTGAT TTTGAGGCGG GGGAGGAGTT TACCTGCGGA 
AGCAACCTGG CATACCTCTA TTTCATCAGT TTCTTTGCAC TCTGTGCCTT TTTGATTATT 
AACTTGTTCA TTGCTGTCAT CATGGATAAC TTTGAGTATC TGACTAGAGA CTGGACTGTG 
CTTGGCACTC ATCACCTCGA TGAGTTTAAA CGAGTCTGGT CAGATTACGA TCCAGAGGCC 
ACGGGACGGA TTAAACATTT AGATGTAGTT ACCATGCTGC GCAGAATTCA GCCTCCACTG 
GGGTTTGGAA AACTCTGTCC ACATCGAGTG GCCTGTAGGA GACTGGTTGC TATGAATGTT 
CCTCTCTATC CTGATGGCAC TGTGTCCTTC AACGCCACCC TATTTGCTCT TGTCAGAACT 
TCACTCAAGA TTAAAACAGA TGGCCCCATT GATCAACAGA ATGAAGAGCT GAGAGCTATT 
ATTAAAAAGA TCTGGAAACG CACCAAACCC AAACTCCTAG ATGAAGTTAT ACCTCCCCCT 
TCAGGCAATG AAGTGACTGC AGGGAAGTTT TATGCCAGCT TTCTAATTCA GGACTACTTT 
AAAAAGTTTC GCAAGAGAAA GGAGAAAGAG AGGAAGAAGA AAGGCAAAGA CAAATCAGCC 
TCTCTCCAGG CTGGGTTACG CACATTACAG GATCTTGCCC CAGAGATGCG ACTAGCTATG 
GCGATGGAGG ATGAGGAAGC ACTGGAGGGG GAGATGATGG AAGATGAGGA GTTCTTTAGG 
AGTGACAGCG TCTTCAGTGA TGTTCCTCCT ACACCAACTA TGTCCACCCC TAGAAGCACG 
CCTATGCCCC CACATCTGGA TCAGACCACA GTAAACATCG AGCTTCCCCC AAGAATACGC 
AATGGAAGCT TAATGCTGGA GGAAGCCCTG TTGGACTCCC GTCCAGCCTC TGCCCTATCT 
GAAAATGAAG TGATAGTTGA ACAGCCTAAC AGGTTGTCTC CCTTACCCAG CAAGTGGACC 
TCCAGTCACA GTAAACTTAC CCCATTGTGG ATACTGAATA CCAACCCAAG ACAAGGAGCA 
CAGAATGACA GCACTGGAGT TTGTGTCTAT CCACCTTCCC CATCTCCGGA TGTAGCCATG 
CAAAATGGAC AAGTCACAGA AGGGGATGTC GTTCCAGGAG GACATGGAGG AGCAACTATG 
GGAGGAGTCA TGGAAGCGGG AGTTGTCCAT GGGGTAGCAA CAGATGGAGT AGTCCATGGG 
GGATCAATCA TAGGAGAAGA AGTAGTCATG GGAGGAGGAG TCACAGAATT GACATACACT 
GCAGACCAAG GTGGTGTGGG CAGTGGGGAC CCTCAGACTG CAGTCTTACC TGAACAACAG 
TGGTTTCCTG AAGTTCCGCC AACACAAGCT GTAGTACCTG ATAGGACCAC TGAATGTGTG 
GCTCCAGTTC AGCCCATCAA CCTTAACGGC AATGGTTACC AAGGAAACAT CCAAGATGCT 
GGCAACTACA ATGGCAGTTT CCCTGGAAAT GGCTACAATG GGAATGGTAC CAATGGAACT 
GATTATCCAG GCAATCGTTG CTCCAGTAAC GGTTATAGCA CCAATGGCTA CAATGGTAAT 
GGTTACAATA GTAATGGATT CAATGGTAAT GGATACAACA ATAATGAATA CAGTGGCAAT 
GGTTTTGCTG CACGGCGAAG AATGCTTCCC CCAACACCAT TAGGCCGGAA GCCAAACTTT 
AACATTCAGT GTTTGCGAAG GCAGGGCAGT AATGAGGATC TCCCAGTCCC TGGCACATAT 
CATCAAAACT CCCCACCCTG CAGGGCTCGC GCTCAGATAA CACAGACACA CAACAGCTTT 
GACTCACGAC GGAGCAGCAT TTCCTCTGGG ATTTCTTCAG CTTCTTGGGC TAACAACCAG 
GCTAGACGGG GTCGCCTCCT CTATGCGCCC CTCATTTTGG TGAACGAGGT GGGGGGCACT 
CAGGCAATGT GGAGTGACAC CAATGGCAGC ACCACTCTTC CAGCATCAGC ACGTCCGGGG 
TGGCTTGGTA GTGCAATGGC AGGTGTGCCC CAGCAGCCTC GAGCATACAC TACTCTCAGG 
GTGCCATCGC AAAACAGCCC TGGATATATA GAGAGAGGAA GCGCTGACAG TCTTGTGGAA 
TCGATTCTGA TTTCTGAAGG CTTGGGTCTA TATGCCAAAG ACCGCAAATT TGTTGACTTC 
GCAAAACGGG AGATTGCAGA TGCCTGTCAA ATGACTGTTG ATGAAATGGA GAACGCCGCA 
ACTGACCTTT TATGTAGAGG AAGGGCAGGG CAACCAACCG GACGCTTCGA TGAGGATCTG 
TCTGATGAGA TGAACTGTGT GATCTCGTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cacna1fa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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