CACNA1F cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following CACNA1F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21305 NM_005183.4
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu99472 XM_017029836.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu21327 NM_001256790.3
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu21801 NM_001256789.3
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu56583 XM_011543983.2
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
View Old Accession Versions >>
OHu21801 NM_001256789.2 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu21305 NM_005183.3 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu21327 NM_001256790.2 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu21305
    Clone ID Related Accession (Same CDS sequence) NM_005183.4 , NM_005183.3
    Accession Version NM_005183.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF201304.1 and AC232271.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_005183.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF201304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
    GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT
    GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG
    GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT
    CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG
    ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG
    GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC
    GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC
    CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT
    GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT
    GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA
    TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC
    GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG
    CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA
    GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC
    TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG
    AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC
    CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC
    GCCGATGACA ACCTTGGTTC TATGGCTGAA GAGGGCCGGG CGGGCCATCG GCCACAGCTG
    GCCGAGCTGA CCAATAGGAG GCGTGGACGT CTGCGCTGGT TCAGTCATTC TACTCGCTCC
    ACACACTCCA CCAGCAGCCA TGCCAGCCTC CCAGCCAGTG ACACCGGTTC CATGACAGAG
    ACCCAAGGCG ATGAGGATGA GGAGGAGGGG GCTCTGGCCA GCTGTACACG CTGCCTAAAC
    AAGATCATGA AAACCAGAGT CTGCCGCCGC CTCCGCCGAG CCAACCGGGT CCTTCGGGCA
    CGCTGCCGTC GGGCAGTGAA GTCCAATGCC TGCTACTGGG CTGTGCTGTT GCTCGTCTTC
    CTCAACACGT TGACCATCGC CTCTGAGCAC CACGGGCAGC CTGTGTGGCT CACCCAGATC
    CAGGAGTATG CCAACAAAGT GTTGCTCTGT CTGTTCACGG TGGAGATGCT TCTCAAATTG
    TACGGTCTGG GCCCCTCTGC CTATGTGTCT TCCTTCTTCA ACCGCTTTGA CTGCTTTGTG
    GTCTGTGGGG GCATCCTAGA GACCACCTTG GTGGAGGTGG GTGCCATGCA GCCCTTGGGC
    ATCTCAGTGC TCCGATGTGT GCGCCTCCTC AGGATCTTTA AGGTCACCAG ACACTGGGCT
    TCTCTGAGCA ATCTGGTGGC ATCCCTGCTC AATTCAATGA AATCCATCGC ATCCTTGCTG
    CTTCTCCTCT TCCTCTTCAT CATTATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC
    AAGTTCAACT TTGACCAGAC CCACACCAAG CGAAGCACCT TTGACACGTT CCCCCAGGCC
    CTCCTCACTG TCTTTCAGAT CCTGACAGGT GAGGACTGGA ACGTGGTCAT GTATGATGGT
    ATCATGGCAT ATGGTGGCCC CTTCTTCCCA GGAATGTTGG TGTGCATCTA TTTCATCATT
    CTCTTCATCT GTGGCAACTA CATCCTGTTG AACGTGTTTC TTGCCATTGC TGTGGACAAC
    CTGGCCAGTG GAGATGCAGG CACTGCCAAG GACAAGGGCG GGGAGAAGAG CAATGAGAAG
    GATCTCCCAC AGGAGAATGA AGGCCTGGTG CCTGGTGTGG AGAAAGAGGA AGAGGAGGGT
    GCAAGGAGGG AAGGAGCAGA CATGGAGGAG GAGGAGGAGG AGGAAGAAGA GGAAGAAGAG
    GAAGAAGAGG AAGAGGGTGC AGGGGGTGTG GAACTCCTGC AGGAAGTTGT ACCCAAGGAG
    AAGGTGGTAC CCATCCCTGA GGGCAGCGCC TTCTTCTGCC TCAGCCAAAC CAACCCGCTG
    AGGAAGGGCT GCCACACCCT CATCCACCAT CATGTCTTCA CCAATCTTAT CCTGGTGTTC
    ATCATCCTCA GCAGTGTGTC CCTGGCCGCT GAGGACCCCA TCCGAGCCCA CTCCTTCCGC
    AACCATATTC TGGGTTACTT CGATTATGCC TTCACCTCCA TTTTCACTGT GGAGATTCTA
    CTAAAGATGA CAGTGTTTGG GGCCTTCCTG CACCGCGGCT CCTTCTGCCG TAGCTGGTTT
    AATATGTTGG ATCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTTGGCAT CCACTCCAGC
    GCCATCTCGG TGGTGAAGAT TCTGCGAGTA CTCCGAGTAC TGCGGCCCCT CCGAGCCATC
    AACAGGGCCA AGGGACTCAA GCATGTGGTG CAGTGTGTAT TTGTGGCCAT CCGGACCATC
    GGAAACATCA TGATTGTCAC CACACTTCTG CAATTTATGT TCGCCTGCAT CGGGGTGCAG
    CTCTTCAAGG GGAAATTCTA CACCTGCACG GACGAGGCCA AACACACCCC TCAAGAATGC
    AAGGGCTCCT TCCTGGTATA CCCAGATGGA GACGTGTCAC GGCCCCTGGT CCGGGAGCGG
    CTCTGGGTCA ACAGTGATTT CAACTTTGAC AATGTCCTTT CAGCCATGAT GGCCCTGTTC
    ACTGTCTCCA CCTTTGAAGG CTGGCCTGCA CTGCTATACA AGGCCATCGA TGCATATGCA
    GAGGACCACG GCCCCATCTA TAATTACCGT GTGGAGATCT CAGTGTTCTT CATTGTCTAC
    ATCATCATCA TTGCGTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CATCACTTTC
    CGTGCCCAGG GCGAGCAGGA GTACCAAAAC TGTGAGCTGG ACAAGAACCA GCGTCAATGT
    GTGGAATATG CCCTCAAGGC CCAGCCACTC CGCCGTTACA TCCCCAAGAA CCCGCATCAG
    TATCGTGTGT GGGCCACTGT GAACTCTGCT GCCTTTGAGT ACCTGATGTT CCTGCTCATC
    CTGCTCAACA CAGTTGCCCT AGCCATGCAG CACTATGAGC AGACTGCTCC CTTCAACTAT
    GCCATGGACA TCCTCAACAT GGTCTTCACT GGCCTCTTCA CTATTGAGAT GGTGCTCAAA
    ATCATCGCCT TCAAGCCCAA GCATTACTTC ACTGATGCCT GGAACACGTT TGACGCTCTT
    ATTGTGGTGG GCAGCATAGT GGATATTGCC GTCACTGAAG TCAATAATGG TGGCCACCTT
    GGCGAGAGCT CTGAGGACAG CTCCCGCATT TCCATTACCT TCTTTCGCCT CTTCCGAGTT
    ATGCGGCTGG TCAAGCTTCT CAGTAAGGGT GAAGGGATCC GCACATTGCT CTGGACATTC
    ATCAAGTCCT TCCAGGCCTT GCCCTATGTG GCTCTTCTCA TCGCAATGAT ATTCTTCATC
    TATGCCGTCA TTGGCATGCA GATGTTCGGC AAGGTGGCTC TTCAGGATGG CACACAGATA
    AACCGAAACA ACAACTTCCA GACCTTTCCA CAGGCTGTGC TGCTTCTGTT CAGGTGTGCC
    ACTGGTGAGG CATGGCAGGA GATAATGCTT GCCAGCCTTC CCGGAAATCG GTGTGATCCT
    GAGTCTGACT TCGGCCCTGG TGAAGAGTTT ACCTGTGGTA GCAATTTTGC CATCGCCTAT
    TTCATCAGCT TCTTCATGCT CTGTGCCTTC CTGATCATAA ATCTCTTTGT GGCTGTGATC
    ATGGACAACT TTGATTATCT CACCAGAGAT TGGTCCATCC TGGGCCCCCA TCACCTTGAT
    GAATTCAAGA GGATCTGGTC TGAATATGAC CCTGGGGCCA AGGGCCGCAT CAAACACTTG
    GATGTGGTTG CCCTGCTGAG ACGTATCCAG CCCCCTCTGG GATTTGGGAA GCTGTGCCCA
    CACCGAGTGG CCTGCAAGAG ACTTGTGGCA ATGAACATGC CCCTCAACTC AGATGGGACG
    GTGACATTCA ACGCCACACT CTTTGCCCTG GTCCGGACAT CCCTGAAGAT CAAAACAGAA
    GGGAACCTGG AGCAAGCCAA CCAGGAGCTG CGGATTGTCA TCAAAAAGAT CTGGAAGCGG
    ATGAAACAGA AGCTGCTAGA TGAGGTCATC CCCCCACCAG ACGAGGAGGA GGTCACCGTG
    GGCAAATTCT ACGCCACATT TCTGATCCAG GACTATTTCC GCAAATTCCG GCGGAGGAAA
    GAAAAAGGGC TACTAGGCAA CGACGCCGCC CCTAGCACCT CTTCCGCCCT TCAGGCTGGT
    CTGCGGAGCC TGCAGGACTT GGGTCCTGAG ATGCGGCAGG CCCTCACCTG TGACACAGAG
    GAGGAGGAAG AAGAGGGGCA GGAGGGAGTG GAGGAGGAAG ATGAAAAGGA CTTGGAAACT
    AACAAAGCCA CGATGGTCTC CCAGCCCTCA GCTCGCCGGG GCTCCGGGAT TTCTGTGTCT
    CTGCCTGTCG GGGACAGACT TCCAGATTCA CTCTCCTTTG GGCCCAGTGA TGATGACAGG
    GGGACTCCCA CCTCCAGTCA GCCCAGTGTG CCCCAGGCTG GATCCAACAC CCACAGGAGA
    GGCTCTGGGG CTCTCATTTT CACCATCCCA GAAGAAGGAA ATTCTCAGCC CAAGGGAACC
    AAAGGGCAAA ACAAGCAAGA TGAGGATGAG GAAGTCCCTG ATCGGCTTTC CTACCTAGAT
    GAGCAGGCAG GGACTCCCCC GTGCTCAGTC CTTTTGCCAC CTCACAGAGC TCAGAGATAC
    ATGGATGGGC ACCTGGTACC ACGCCGCCGT CTGCTGCCCC CCACACCTGC AGGTCGGAAG
    CCCTCCTTCA CCATCCAGTG TCTGCAGCGC CAGGGCAGTT GTGAGGATTT ACCCATCCCA
    GGCACCTATC ATCGTGGGCG AAATTCAGGG CCCAATAGGG CTCAGGGTTC CTGGGCAACA
    CCACCTCAGC GGGGTCGGCT CCTGTATGCC CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG
    GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC CCACTGCGCA CCTTCACCTG TCTGCACGTG
    CCTGGAACCC ACTCGGACCC CAGCCATGGG AAGAGGGGCA GTGCCGACAG CTTGGTGGAG
    GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC TTTGCTCGAG ACCCACGTTT CGTGGCCCTG
    GCCAAGCAGG AGATTGCAGA TGCGTGTCGC CTGACGCTGG ATGAGATGGA CAATGCTGCC
    AGTGACCTGC TGGCACAGGG AACCAGCTCT CTCTATAGCG ACGAGGAGTC CATCCTCTCC
    CGCTTCGATG AGGAGGACTT GGGAGACGAG ATGGCCTGCG TCCACGCCCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005174.2
    CDS32..5965
    Translation

    Target ORF information:

    RefSeq Version NM_005183.4
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005183.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
    GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT
    GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG
    GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT
    CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG
    ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG
    GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC
    GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC
    CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT
    GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT
    GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA
    TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC
    GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG
    CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA
    GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC
    TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG
    AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC
    CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC
    GCCGATGACA ACCTTGGTTC TATGGCTGAA GAGGGCCGGG CGGGCCATCG GCCACAGCTG
    GCCGAGCTGA CCAATAGGAG GCGTGGACGT CTGCGCTGGT TCAGTCATTC TACTCGCTCC
    ACACACTCCA CCAGCAGCCA TGCCAGCCTC CCAGCCAGTG ACACCGGTTC CATGACAGAG
    ACCCAAGGCG ATGAGGATGA GGAGGAGGGG GCTCTGGCCA GCTGTACACG CTGCCTAAAC
    AAGATCATGA AAACCAGAGT CTGCCGCCGC CTCCGCCGAG CCAACCGGGT CCTTCGGGCA
    CGCTGCCGTC GGGCAGTGAA GTCCAATGCC TGCTACTGGG CTGTGCTGTT GCTCGTCTTC
    CTCAACACGT TGACCATCGC CTCTGAGCAC CACGGGCAGC CTGTGTGGCT CACCCAGATC
    CAGGAGTATG CCAACAAAGT GTTGCTCTGT CTGTTCACGG TGGAGATGCT TCTCAAATTG
    TACGGTCTGG GCCCCTCTGC CTATGTGTCT TCCTTCTTCA ACCGCTTTGA CTGCTTTGTG
    GTCTGTGGGG GCATCCTAGA GACCACCTTG GTGGAGGTGG GTGCCATGCA GCCCTTGGGC
    ATCTCAGTGC TCCGATGTGT GCGCCTCCTC AGGATCTTTA AGGTCACCAG ACACTGGGCT
    TCTCTGAGCA ATCTGGTGGC ATCCCTGCTC AATTCAATGA AATCCATCGC ATCCTTGCTG
    CTTCTCCTCT TCCTCTTCAT CATTATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC
    AAGTTCAACT TTGACCAGAC CCACACCAAG CGAAGCACCT TTGACACGTT CCCCCAGGCC
    CTCCTCACTG TCTTTCAGAT CCTGACAGGT GAGGACTGGA ACGTGGTCAT GTATGATGGT
    ATCATGGCAT ATGGTGGCCC CTTCTTCCCA GGAATGTTGG TGTGCATCTA TTTCATCATT
    CTCTTCATCT GTGGCAACTA CATCCTGTTG AACGTGTTTC TTGCCATTGC TGTGGACAAC
    CTGGCCAGTG GAGATGCAGG CACTGCCAAG GACAAGGGCG GGGAGAAGAG CAATGAGAAG
    GATCTCCCAC AGGAGAATGA AGGCCTGGTG CCTGGTGTGG AGAAAGAGGA AGAGGAGGGT
    GCAAGGAGGG AAGGAGCAGA CATGGAGGAG GAGGAGGAGG AGGAAGAAGA GGAAGAAGAG
    GAAGAAGAGG AAGAGGGTGC AGGGGGTGTG GAACTCCTGC AGGAAGTTGT ACCCAAGGAG
    AAGGTGGTAC CCATCCCTGA GGGCAGCGCC TTCTTCTGCC TCAGCCAAAC CAACCCGCTG
    AGGAAGGGCT GCCACACCCT CATCCACCAT CATGTCTTCA CCAATCTTAT CCTGGTGTTC
    ATCATCCTCA GCAGTGTGTC CCTGGCCGCT GAGGACCCCA TCCGAGCCCA CTCCTTCCGC
    AACCATATTC TGGGTTACTT CGATTATGCC TTCACCTCCA TTTTCACTGT GGAGATTCTA
    CTAAAGATGA CAGTGTTTGG GGCCTTCCTG CACCGCGGCT CCTTCTGCCG TAGCTGGTTT
    AATATGTTGG ATCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTTGGCAT CCACTCCAGC
    GCCATCTCGG TGGTGAAGAT TCTGCGAGTA CTCCGAGTAC TGCGGCCCCT CCGAGCCATC
    AACAGGGCCA AGGGACTCAA GCATGTGGTG CAGTGTGTAT TTGTGGCCAT CCGGACCATC
    GGAAACATCA TGATTGTCAC CACACTTCTG CAATTTATGT TCGCCTGCAT CGGGGTGCAG
    CTCTTCAAGG GGAAATTCTA CACCTGCACG GACGAGGCCA AACACACCCC TCAAGAATGC
    AAGGGCTCCT TCCTGGTATA CCCAGATGGA GACGTGTCAC GGCCCCTGGT CCGGGAGCGG
    CTCTGGGTCA ACAGTGATTT CAACTTTGAC AATGTCCTTT CAGCCATGAT GGCCCTGTTC
    ACTGTCTCCA CCTTTGAAGG CTGGCCTGCA CTGCTATACA AGGCCATCGA TGCATATGCA
    GAGGACCACG GCCCCATCTA TAATTACCGT GTGGAGATCT CAGTGTTCTT CATTGTCTAC
    ATCATCATCA TTGCGTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CATCACTTTC
    CGTGCCCAGG GCGAGCAGGA GTACCAAAAC TGTGAGCTGG ACAAGAACCA GCGTCAATGT
    GTGGAATATG CCCTCAAGGC CCAGCCACTC CGCCGTTACA TCCCCAAGAA CCCGCATCAG
    TATCGTGTGT GGGCCACTGT GAACTCTGCT GCCTTTGAGT ACCTGATGTT CCTGCTCATC
    CTGCTCAACA CAGTTGCCCT AGCCATGCAG CACTATGAGC AGACTGCTCC CTTCAACTAT
    GCCATGGACA TCCTCAACAT GGTCTTCACT GGCCTCTTCA CTATTGAGAT GGTGCTCAAA
    ATCATCGCCT TCAAGCCCAA GCATTACTTC ACTGATGCCT GGAACACGTT TGACGCTCTT
    ATTGTGGTGG GCAGCATAGT GGATATTGCC GTCACTGAAG TCAATAATGG TGGCCACCTT
    GGCGAGAGCT CTGAGGACAG CTCCCGCATT TCCATTACCT TCTTTCGCCT CTTCCGAGTT
    ATGCGGCTGG TCAAGCTTCT CAGTAAGGGT GAAGGGATCC GCACATTGCT CTGGACATTC
    ATCAAGTCCT TCCAGGCCTT GCCCTATGTG GCTCTTCTCA TCGCAATGAT ATTCTTCATC
    TATGCCGTCA TTGGCATGCA GATGTTCGGC AAGGTGGCTC TTCAGGATGG CACACAGATA
    AACCGAAACA ACAACTTCCA GACCTTTCCA CAGGCTGTGC TGCTTCTGTT CAGGTGTGCC
    ACTGGTGAGG CATGGCAGGA GATAATGCTT GCCAGCCTTC CCGGAAATCG GTGTGATCCT
    GAGTCTGACT TCGGCCCTGG TGAAGAGTTT ACCTGTGGTA GCAATTTTGC CATCGCCTAT
    TTCATCAGCT TCTTCATGCT CTGTGCCTTC CTGATCATAA ATCTCTTTGT GGCTGTGATC
    ATGGACAACT TTGATTATCT CACCAGAGAT TGGTCCATCC TGGGCCCCCA TCACCTTGAT
    GAATTCAAGA GGATCTGGTC TGAATATGAC CCTGGGGCCA AGGGCCGCAT CAAACACTTG
    GATGTGGTTG CCCTGCTGAG ACGTATCCAG CCCCCTCTGG GATTTGGGAA GCTGTGCCCA
    CACCGAGTGG CCTGCAAGAG ACTTGTGGCA ATGAACATGC CCCTCAACTC AGATGGGACG
    GTGACATTCA ACGCCACACT CTTTGCCCTG GTCCGGACAT CCCTGAAGAT CAAAACAGAA
    GGGAACCTGG AGCAAGCCAA CCAGGAGCTG CGGATTGTCA TCAAAAAGAT CTGGAAGCGG
    ATGAAACAGA AGCTGCTAGA TGAGGTCATC CCCCCACCAG ACGAGGAGGA GGTCACCGTG
    GGCAAATTCT ACGCCACATT TCTGATCCAG GACTATTTCC GCAAATTCCG GCGGAGGAAA
    GAAAAAGGGC TACTAGGCAA CGACGCCGCC CCTAGCACCT CTTCCGCCCT TCAGGCTGGT
    CTGCGGAGCC TGCAGGACTT GGGTCCTGAG ATGCGGCAGG CCCTCACCTG TGACACAGAG
    GAGGAGGAAG AAGAGGGGCA GGAGGGAGTG GAGGAGGAAG ATGAAAAGGA CTTGGAAACT
    AACAAAGCCA CGATGGTCTC CCAGCCCTCA GCTCGCCGGG GCTCCGGGAT TTCTGTGTCT
    CTGCCTGTCG GGGACAGACT TCCAGATTCA CTCTCCTTTG GGCCCAGTGA TGATGACAGG
    GGGACTCCCA CCTCCAGTCA GCCCAGTGTG CCCCAGGCTG GATCCAACAC CCACAGGAGA
    GGCTCTGGGG CTCTCATTTT CACCATCCCA GAAGAAGGAA ATTCTCAGCC CAAGGGAACC
    AAAGGGCAAA ACAAGCAAGA TGAGGATGAG GAAGTCCCTG ATCGGCTTTC CTACCTAGAT
    GAGCAGGCAG GGACTCCCCC GTGCTCAGTC CTTTTGCCAC CTCACAGAGC TCAGAGATAC
    ATGGATGGGC ACCTGGTACC ACGCCGCCGT CTGCTGCCCC CCACACCTGC AGGTCGGAAG
    CCCTCCTTCA CCATCCAGTG TCTGCAGCGC CAGGGCAGTT GTGAGGATTT ACCCATCCCA
    GGCACCTATC ATCGTGGGCG AAATTCAGGG CCCAATAGGG CTCAGGGTTC CTGGGCAACA
    CCACCTCAGC GGGGTCGGCT CCTGTATGCC CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG
    GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC CCACTGCGCA CCTTCACCTG TCTGCACGTG
    CCTGGAACCC ACTCGGACCC CAGCCATGGG AAGAGGGGCA GTGCCGACAG CTTGGTGGAG
    GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC TTTGCTCGAG ACCCACGTTT CGTGGCCCTG
    GCCAAGCAGG AGATTGCAGA TGCGTGTCGC CTGACGCTGG ATGAGATGGA CAATGCTGCC
    AGTGACCTGC TGGCACAGGG AACCAGCTCT CTCTATAGCG ACGAGGAGTC CATCCTCTCC
    CGCTTCGATG AGGAGGACTT GGGAGACGAG ATGGCCTGCG TCCACGCCCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21305
    Clone ID Related Accession (Same CDS sequence) NM_005183.4 , NM_005183.3
    Accession Version NM_005183.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF201304.1 and AC232271.2. This sequence is a reference standard in the RefSeqGene project. On Aug 21, 2013 this sequence version replaced NM_005183.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF201304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
    GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT
    GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG
    GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT
    CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG
    ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG
    GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC
    GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC
    CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT
    GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT
    GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA
    TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC
    GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG
    CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA
    GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC
    TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG
    AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC
    CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC
    GCCGATGACA ACCTTGGTTC TATGGCTGAA GAGGGCCGGG CGGGCCATCG GCCACAGCTG
    GCCGAGCTGA CCAATAGGAG GCGTGGACGT CTGCGCTGGT TCAGTCATTC TACTCGCTCC
    ACACACTCCA CCAGCAGCCA TGCCAGCCTC CCAGCCAGTG ACACCGGTTC CATGACAGAG
    ACCCAAGGCG ATGAGGATGA GGAGGAGGGG GCTCTGGCCA GCTGTACACG CTGCCTAAAC
    AAGATCATGA AAACCAGAGT CTGCCGCCGC CTCCGCCGAG CCAACCGGGT CCTTCGGGCA
    CGCTGCCGTC GGGCAGTGAA GTCCAATGCC TGCTACTGGG CTGTGCTGTT GCTCGTCTTC
    CTCAACACGT TGACCATCGC CTCTGAGCAC CACGGGCAGC CTGTGTGGCT CACCCAGATC
    CAGGAGTATG CCAACAAAGT GTTGCTCTGT CTGTTCACGG TGGAGATGCT TCTCAAATTG
    TACGGTCTGG GCCCCTCTGC CTATGTGTCT TCCTTCTTCA ACCGCTTTGA CTGCTTTGTG
    GTCTGTGGGG GCATCCTAGA GACCACCTTG GTGGAGGTGG GTGCCATGCA GCCCTTGGGC
    ATCTCAGTGC TCCGATGTGT GCGCCTCCTC AGGATCTTTA AGGTCACCAG ACACTGGGCT
    TCTCTGAGCA ATCTGGTGGC ATCCCTGCTC AATTCAATGA AATCCATCGC ATCCTTGCTG
    CTTCTCCTCT TCCTCTTCAT CATTATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC
    AAGTTCAACT TTGACCAGAC CCACACCAAG CGAAGCACCT TTGACACGTT CCCCCAGGCC
    CTCCTCACTG TCTTTCAGAT CCTGACAGGT GAGGACTGGA ACGTGGTCAT GTATGATGGT
    ATCATGGCAT ATGGTGGCCC CTTCTTCCCA GGAATGTTGG TGTGCATCTA TTTCATCATT
    CTCTTCATCT GTGGCAACTA CATCCTGTTG AACGTGTTTC TTGCCATTGC TGTGGACAAC
    CTGGCCAGTG GAGATGCAGG CACTGCCAAG GACAAGGGCG GGGAGAAGAG CAATGAGAAG
    GATCTCCCAC AGGAGAATGA AGGCCTGGTG CCTGGTGTGG AGAAAGAGGA AGAGGAGGGT
    GCAAGGAGGG AAGGAGCAGA CATGGAGGAG GAGGAGGAGG AGGAAGAAGA GGAAGAAGAG
    GAAGAAGAGG AAGAGGGTGC AGGGGGTGTG GAACTCCTGC AGGAAGTTGT ACCCAAGGAG
    AAGGTGGTAC CCATCCCTGA GGGCAGCGCC TTCTTCTGCC TCAGCCAAAC CAACCCGCTG
    AGGAAGGGCT GCCACACCCT CATCCACCAT CATGTCTTCA CCAATCTTAT CCTGGTGTTC
    ATCATCCTCA GCAGTGTGTC CCTGGCCGCT GAGGACCCCA TCCGAGCCCA CTCCTTCCGC
    AACCATATTC TGGGTTACTT CGATTATGCC TTCACCTCCA TTTTCACTGT GGAGATTCTA
    CTAAAGATGA CAGTGTTTGG GGCCTTCCTG CACCGCGGCT CCTTCTGCCG TAGCTGGTTT
    AATATGTTGG ATCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTTGGCAT CCACTCCAGC
    GCCATCTCGG TGGTGAAGAT TCTGCGAGTA CTCCGAGTAC TGCGGCCCCT CCGAGCCATC
    AACAGGGCCA AGGGACTCAA GCATGTGGTG CAGTGTGTAT TTGTGGCCAT CCGGACCATC
    GGAAACATCA TGATTGTCAC CACACTTCTG CAATTTATGT TCGCCTGCAT CGGGGTGCAG
    CTCTTCAAGG GGAAATTCTA CACCTGCACG GACGAGGCCA AACACACCCC TCAAGAATGC
    AAGGGCTCCT TCCTGGTATA CCCAGATGGA GACGTGTCAC GGCCCCTGGT CCGGGAGCGG
    CTCTGGGTCA ACAGTGATTT CAACTTTGAC AATGTCCTTT CAGCCATGAT GGCCCTGTTC
    ACTGTCTCCA CCTTTGAAGG CTGGCCTGCA CTGCTATACA AGGCCATCGA TGCATATGCA
    GAGGACCACG GCCCCATCTA TAATTACCGT GTGGAGATCT CAGTGTTCTT CATTGTCTAC
    ATCATCATCA TTGCGTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CATCACTTTC
    CGTGCCCAGG GCGAGCAGGA GTACCAAAAC TGTGAGCTGG ACAAGAACCA GCGTCAATGT
    GTGGAATATG CCCTCAAGGC CCAGCCACTC CGCCGTTACA TCCCCAAGAA CCCGCATCAG
    TATCGTGTGT GGGCCACTGT GAACTCTGCT GCCTTTGAGT ACCTGATGTT CCTGCTCATC
    CTGCTCAACA CAGTTGCCCT AGCCATGCAG CACTATGAGC AGACTGCTCC CTTCAACTAT
    GCCATGGACA TCCTCAACAT GGTCTTCACT GGCCTCTTCA CTATTGAGAT GGTGCTCAAA
    ATCATCGCCT TCAAGCCCAA GCATTACTTC ACTGATGCCT GGAACACGTT TGACGCTCTT
    ATTGTGGTGG GCAGCATAGT GGATATTGCC GTCACTGAAG TCAATAATGG TGGCCACCTT
    GGCGAGAGCT CTGAGGACAG CTCCCGCATT TCCATTACCT TCTTTCGCCT CTTCCGAGTT
    ATGCGGCTGG TCAAGCTTCT CAGTAAGGGT GAAGGGATCC GCACATTGCT CTGGACATTC
    ATCAAGTCCT TCCAGGCCTT GCCCTATGTG GCTCTTCTCA TCGCAATGAT ATTCTTCATC
    TATGCCGTCA TTGGCATGCA GATGTTCGGC AAGGTGGCTC TTCAGGATGG CACACAGATA
    AACCGAAACA ACAACTTCCA GACCTTTCCA CAGGCTGTGC TGCTTCTGTT CAGGTGTGCC
    ACTGGTGAGG CATGGCAGGA GATAATGCTT GCCAGCCTTC CCGGAAATCG GTGTGATCCT
    GAGTCTGACT TCGGCCCTGG TGAAGAGTTT ACCTGTGGTA GCAATTTTGC CATCGCCTAT
    TTCATCAGCT TCTTCATGCT CTGTGCCTTC CTGATCATAA ATCTCTTTGT GGCTGTGATC
    ATGGACAACT TTGATTATCT CACCAGAGAT TGGTCCATCC TGGGCCCCCA TCACCTTGAT
    GAATTCAAGA GGATCTGGTC TGAATATGAC CCTGGGGCCA AGGGCCGCAT CAAACACTTG
    GATGTGGTTG CCCTGCTGAG ACGTATCCAG CCCCCTCTGG GATTTGGGAA GCTGTGCCCA
    CACCGAGTGG CCTGCAAGAG ACTTGTGGCA ATGAACATGC CCCTCAACTC AGATGGGACG
    GTGACATTCA ACGCCACACT CTTTGCCCTG GTCCGGACAT CCCTGAAGAT CAAAACAGAA
    GGGAACCTGG AGCAAGCCAA CCAGGAGCTG CGGATTGTCA TCAAAAAGAT CTGGAAGCGG
    ATGAAACAGA AGCTGCTAGA TGAGGTCATC CCCCCACCAG ACGAGGAGGA GGTCACCGTG
    GGCAAATTCT ACGCCACATT TCTGATCCAG GACTATTTCC GCAAATTCCG GCGGAGGAAA
    GAAAAAGGGC TACTAGGCAA CGACGCCGCC CCTAGCACCT CTTCCGCCCT TCAGGCTGGT
    CTGCGGAGCC TGCAGGACTT GGGTCCTGAG ATGCGGCAGG CCCTCACCTG TGACACAGAG
    GAGGAGGAAG AAGAGGGGCA GGAGGGAGTG GAGGAGGAAG ATGAAAAGGA CTTGGAAACT
    AACAAAGCCA CGATGGTCTC CCAGCCCTCA GCTCGCCGGG GCTCCGGGAT TTCTGTGTCT
    CTGCCTGTCG GGGACAGACT TCCAGATTCA CTCTCCTTTG GGCCCAGTGA TGATGACAGG
    GGGACTCCCA CCTCCAGTCA GCCCAGTGTG CCCCAGGCTG GATCCAACAC CCACAGGAGA
    GGCTCTGGGG CTCTCATTTT CACCATCCCA GAAGAAGGAA ATTCTCAGCC CAAGGGAACC
    AAAGGGCAAA ACAAGCAAGA TGAGGATGAG GAAGTCCCTG ATCGGCTTTC CTACCTAGAT
    GAGCAGGCAG GGACTCCCCC GTGCTCAGTC CTTTTGCCAC CTCACAGAGC TCAGAGATAC
    ATGGATGGGC ACCTGGTACC ACGCCGCCGT CTGCTGCCCC CCACACCTGC AGGTCGGAAG
    CCCTCCTTCA CCATCCAGTG TCTGCAGCGC CAGGGCAGTT GTGAGGATTT ACCCATCCCA
    GGCACCTATC ATCGTGGGCG AAATTCAGGG CCCAATAGGG CTCAGGGTTC CTGGGCAACA
    CCACCTCAGC GGGGTCGGCT CCTGTATGCC CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG
    GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC CCACTGCGCA CCTTCACCTG TCTGCACGTG
    CCTGGAACCC ACTCGGACCC CAGCCATGGG AAGAGGGGCA GTGCCGACAG CTTGGTGGAG
    GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC TTTGCTCGAG ACCCACGTTT CGTGGCCCTG
    GCCAAGCAGG AGATTGCAGA TGCGTGTCGC CTGACGCTGG ATGAGATGGA CAATGCTGCC
    AGTGACCTGC TGGCACAGGG AACCAGCTCT CTCTATAGCG ACGAGGAGTC CATCCTCTCC
    CGCTTCGATG AGGAGGACTT GGGAGACGAG ATGGCCTGCG TCCACGCCCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005174.2
    CDS63..5996
    Translation

    Target ORF information:

    RefSeq Version NM_005183.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005183.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
    GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT
    GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG
    GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT
    CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG
    ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG
    GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC
    GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC
    CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT
    GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT
    GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA
    TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC
    GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG
    CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA
    GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC
    TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG
    AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC
    CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC
    GCCGATGACA ACCTTGGTTC TATGGCTGAA GAGGGCCGGG CGGGCCATCG GCCACAGCTG
    GCCGAGCTGA CCAATAGGAG GCGTGGACGT CTGCGCTGGT TCAGTCATTC TACTCGCTCC
    ACACACTCCA CCAGCAGCCA TGCCAGCCTC CCAGCCAGTG ACACCGGTTC CATGACAGAG
    ACCCAAGGCG ATGAGGATGA GGAGGAGGGG GCTCTGGCCA GCTGTACACG CTGCCTAAAC
    AAGATCATGA AAACCAGAGT CTGCCGCCGC CTCCGCCGAG CCAACCGGGT CCTTCGGGCA
    CGCTGCCGTC GGGCAGTGAA GTCCAATGCC TGCTACTGGG CTGTGCTGTT GCTCGTCTTC
    CTCAACACGT TGACCATCGC CTCTGAGCAC CACGGGCAGC CTGTGTGGCT CACCCAGATC
    CAGGAGTATG CCAACAAAGT GTTGCTCTGT CTGTTCACGG TGGAGATGCT TCTCAAATTG
    TACGGTCTGG GCCCCTCTGC CTATGTGTCT TCCTTCTTCA ACCGCTTTGA CTGCTTTGTG
    GTCTGTGGGG GCATCCTAGA GACCACCTTG GTGGAGGTGG GTGCCATGCA GCCCTTGGGC
    ATCTCAGTGC TCCGATGTGT GCGCCTCCTC AGGATCTTTA AGGTCACCAG ACACTGGGCT
    TCTCTGAGCA ATCTGGTGGC ATCCCTGCTC AATTCAATGA AATCCATCGC ATCCTTGCTG
    CTTCTCCTCT TCCTCTTCAT CATTATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC
    AAGTTCAACT TTGACCAGAC CCACACCAAG CGAAGCACCT TTGACACGTT CCCCCAGGCC
    CTCCTCACTG TCTTTCAGAT CCTGACAGGT GAGGACTGGA ACGTGGTCAT GTATGATGGT
    ATCATGGCAT ATGGTGGCCC CTTCTTCCCA GGAATGTTGG TGTGCATCTA TTTCATCATT
    CTCTTCATCT GTGGCAACTA CATCCTGTTG AACGTGTTTC TTGCCATTGC TGTGGACAAC
    CTGGCCAGTG GAGATGCAGG CACTGCCAAG GACAAGGGCG GGGAGAAGAG CAATGAGAAG
    GATCTCCCAC AGGAGAATGA AGGCCTGGTG CCTGGTGTGG AGAAAGAGGA AGAGGAGGGT
    GCAAGGAGGG AAGGAGCAGA CATGGAGGAG GAGGAGGAGG AGGAAGAAGA GGAAGAAGAG
    GAAGAAGAGG AAGAGGGTGC AGGGGGTGTG GAACTCCTGC AGGAAGTTGT ACCCAAGGAG
    AAGGTGGTAC CCATCCCTGA GGGCAGCGCC TTCTTCTGCC TCAGCCAAAC CAACCCGCTG
    AGGAAGGGCT GCCACACCCT CATCCACCAT CATGTCTTCA CCAATCTTAT CCTGGTGTTC
    ATCATCCTCA GCAGTGTGTC CCTGGCCGCT GAGGACCCCA TCCGAGCCCA CTCCTTCCGC
    AACCATATTC TGGGTTACTT CGATTATGCC TTCACCTCCA TTTTCACTGT GGAGATTCTA
    CTAAAGATGA CAGTGTTTGG GGCCTTCCTG CACCGCGGCT CCTTCTGCCG TAGCTGGTTT
    AATATGTTGG ATCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTTGGCAT CCACTCCAGC
    GCCATCTCGG TGGTGAAGAT TCTGCGAGTA CTCCGAGTAC TGCGGCCCCT CCGAGCCATC
    AACAGGGCCA AGGGACTCAA GCATGTGGTG CAGTGTGTAT TTGTGGCCAT CCGGACCATC
    GGAAACATCA TGATTGTCAC CACACTTCTG CAATTTATGT TCGCCTGCAT CGGGGTGCAG
    CTCTTCAAGG GGAAATTCTA CACCTGCACG GACGAGGCCA AACACACCCC TCAAGAATGC
    AAGGGCTCCT TCCTGGTATA CCCAGATGGA GACGTGTCAC GGCCCCTGGT CCGGGAGCGG
    CTCTGGGTCA ACAGTGATTT CAACTTTGAC AATGTCCTTT CAGCCATGAT GGCCCTGTTC
    ACTGTCTCCA CCTTTGAAGG CTGGCCTGCA CTGCTATACA AGGCCATCGA TGCATATGCA
    GAGGACCACG GCCCCATCTA TAATTACCGT GTGGAGATCT CAGTGTTCTT CATTGTCTAC
    ATCATCATCA TTGCGTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CATCACTTTC
    CGTGCCCAGG GCGAGCAGGA GTACCAAAAC TGTGAGCTGG ACAAGAACCA GCGTCAATGT
    GTGGAATATG CCCTCAAGGC CCAGCCACTC CGCCGTTACA TCCCCAAGAA CCCGCATCAG
    TATCGTGTGT GGGCCACTGT GAACTCTGCT GCCTTTGAGT ACCTGATGTT CCTGCTCATC
    CTGCTCAACA CAGTTGCCCT AGCCATGCAG CACTATGAGC AGACTGCTCC CTTCAACTAT
    GCCATGGACA TCCTCAACAT GGTCTTCACT GGCCTCTTCA CTATTGAGAT GGTGCTCAAA
    ATCATCGCCT TCAAGCCCAA GCATTACTTC ACTGATGCCT GGAACACGTT TGACGCTCTT
    ATTGTGGTGG GCAGCATAGT GGATATTGCC GTCACTGAAG TCAATAATGG TGGCCACCTT
    GGCGAGAGCT CTGAGGACAG CTCCCGCATT TCCATTACCT TCTTTCGCCT CTTCCGAGTT
    ATGCGGCTGG TCAAGCTTCT CAGTAAGGGT GAAGGGATCC GCACATTGCT CTGGACATTC
    ATCAAGTCCT TCCAGGCCTT GCCCTATGTG GCTCTTCTCA TCGCAATGAT ATTCTTCATC
    TATGCCGTCA TTGGCATGCA GATGTTCGGC AAGGTGGCTC TTCAGGATGG CACACAGATA
    AACCGAAACA ACAACTTCCA GACCTTTCCA CAGGCTGTGC TGCTTCTGTT CAGGTGTGCC
    ACTGGTGAGG CATGGCAGGA GATAATGCTT GCCAGCCTTC CCGGAAATCG GTGTGATCCT
    GAGTCTGACT TCGGCCCTGG TGAAGAGTTT ACCTGTGGTA GCAATTTTGC CATCGCCTAT
    TTCATCAGCT TCTTCATGCT CTGTGCCTTC CTGATCATAA ATCTCTTTGT GGCTGTGATC
    ATGGACAACT TTGATTATCT CACCAGAGAT TGGTCCATCC TGGGCCCCCA TCACCTTGAT
    GAATTCAAGA GGATCTGGTC TGAATATGAC CCTGGGGCCA AGGGCCGCAT CAAACACTTG
    GATGTGGTTG CCCTGCTGAG ACGTATCCAG CCCCCTCTGG GATTTGGGAA GCTGTGCCCA
    CACCGAGTGG CCTGCAAGAG ACTTGTGGCA ATGAACATGC CCCTCAACTC AGATGGGACG
    GTGACATTCA ACGCCACACT CTTTGCCCTG GTCCGGACAT CCCTGAAGAT CAAAACAGAA
    GGGAACCTGG AGCAAGCCAA CCAGGAGCTG CGGATTGTCA TCAAAAAGAT CTGGAAGCGG
    ATGAAACAGA AGCTGCTAGA TGAGGTCATC CCCCCACCAG ACGAGGAGGA GGTCACCGTG
    GGCAAATTCT ACGCCACATT TCTGATCCAG GACTATTTCC GCAAATTCCG GCGGAGGAAA
    GAAAAAGGGC TACTAGGCAA CGACGCCGCC CCTAGCACCT CTTCCGCCCT TCAGGCTGGT
    CTGCGGAGCC TGCAGGACTT GGGTCCTGAG ATGCGGCAGG CCCTCACCTG TGACACAGAG
    GAGGAGGAAG AAGAGGGGCA GGAGGGAGTG GAGGAGGAAG ATGAAAAGGA CTTGGAAACT
    AACAAAGCCA CGATGGTCTC CCAGCCCTCA GCTCGCCGGG GCTCCGGGAT TTCTGTGTCT
    CTGCCTGTCG GGGACAGACT TCCAGATTCA CTCTCCTTTG GGCCCAGTGA TGATGACAGG
    GGGACTCCCA CCTCCAGTCA GCCCAGTGTG CCCCAGGCTG GATCCAACAC CCACAGGAGA
    GGCTCTGGGG CTCTCATTTT CACCATCCCA GAAGAAGGAA ATTCTCAGCC CAAGGGAACC
    AAAGGGCAAA ACAAGCAAGA TGAGGATGAG GAAGTCCCTG ATCGGCTTTC CTACCTAGAT
    GAGCAGGCAG GGACTCCCCC GTGCTCAGTC CTTTTGCCAC CTCACAGAGC TCAGAGATAC
    ATGGATGGGC ACCTGGTACC ACGCCGCCGT CTGCTGCCCC CCACACCTGC AGGTCGGAAG
    CCCTCCTTCA CCATCCAGTG TCTGCAGCGC CAGGGCAGTT GTGAGGATTT ACCCATCCCA
    GGCACCTATC ATCGTGGGCG AAATTCAGGG CCCAATAGGG CTCAGGGTTC CTGGGCAACA
    CCACCTCAGC GGGGTCGGCT CCTGTATGCC CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG
    GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC CCACTGCGCA CCTTCACCTG TCTGCACGTG
    CCTGGAACCC ACTCGGACCC CAGCCATGGG AAGAGGGGCA GTGCCGACAG CTTGGTGGAG
    GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC TTTGCTCGAG ACCCACGTTT CGTGGCCCTG
    GCCAAGCAGG AGATTGCAGA TGCGTGTCGC CTGACGCTGG ATGAGATGGA CAATGCTGCC
    AGTGACCTGC TGGCACAGGG AACCAGCTCT CTCTATAGCG ACGAGGAGTC CATCCTCTCC
    CGCTTCGATG AGGAGGACTT GGGAGACGAG ATGGCCTGCG TCCACGCCCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99472
    Clone ID Related Accession (Same CDS sequence) XM_017029836.1
    Accession Version XM_017029836.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3084bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACAGTGT TTGGGGCCTT CCTGCACCGC GGCTCCTTCT GCCGTAGCTG GTTTAATATG 
    TTGGATCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTTG GCATCCACTC CAGCGCCATC
    TCGGTGGTGA AGATTCTGCG AGTACTCCGA GTACTGCGGC CCCTCCGAGC CATCAACAGG
    GCCAAGGGAC TCAAGCATGT GGTGCAGTGT GTATTTGTGG CCATCCGGAC CATCGGAAAC
    ATCATGATTG TCACCACACT TCTGCAATTT ATGTTCGCCT GCATCGGGGT GCAGCTCTTC
    AAGGGGAAAT TCTACACCTG CACGGACGAG GCCAAACACA CCCCTCAAGA ATGCAAGGGC
    TCCTTCCTGG TATACCCAGA TGGAGACGTG TCACGGCCCC TGGTCCGGGA GCGGCTCTGG
    GTCAACAGTG ATTTCAACTT TGACAATGTC CTTTCAGCCA TGATGGCCCT GTTCACTGTC
    TCCACCTTTG AAGGCTGGCC TGCACTGCTA TACAAGGCCA TCGATGCATA TGCAGAGGAC
    CACGGCCCCA TCTATAATTA CCGTGTGGAG ATCTCAGTGT TCTTCATTGT CTACATCATC
    ATCATTGCGT TCTTCATGAT GAACATCTTC GTGGGCTTCG TCATCATCAC TTTCCGTGCC
    CAGGGCGAGC AGGAGTACCA AAACTGTGAG CTGGACAAGA ACCAGCGTCA ATGTGTGGAA
    TATGCCCTCA AGGCCCAGCC ACTCCGCCGT TACATCCCCA AGAACCCGCA TCAGTATCGT
    GTGTGGGCCA CTGTGAACTC TGCTGCCTTT GAGTACCTGA TGTTCCTGCT CATCCTGCTC
    AACACAGTTG CCCTAGCCAT GCAGCACTAT GAGCAGACTG CTCCCTTCAA CTATGCCATG
    GACATCCTCA ACATGGTCTT CACTGGCCTC TTCACTATTG AGATGGTGCT CAAAATCATC
    GCCTTCAAGC CCAAGCATTA CTTCACTGAT GCCTGGAACA CGTTTGACGC TCTTATTGTG
    GTGGGCAGCA TAGTGGATAT TGCCGTCACT GAAGTCAATA GCTCTGAGGA CAGCTCCCGC
    ATTTCCATTA CCTTCTTTCG CCTCTTCCGA GTTATGCGGC TGGTCAAGCT TCTCAGTAAG
    GGTGAAGGGA TCCGCACATT GCTCTGGACA TTCATCAAGT CCTTCCAGGC CTTGCCCTAT
    GTGGCTCTTC TCATCGCAAT GATATTCTTC ATCTATGCCG TCATTGGCAT GCAGATGTTC
    GGCAAGGTGG CTCTTCAGGA TGGCACACAG ATAAACCGAA ACAACAACTT CCAGACCTTT
    CCACAGGCTG TGCTGCTTCT GTTCAGGTGT GCCACTGGTG AGGCATGGCA GGAGATAATG
    CTTGCCAGCC TTCCCGGAAA TCGGTGTGAT CCTGAGTCTG ACTTCGGCCC TGGTGAAGAG
    TTTACCTGTG GTAGCAATTT TGCCATCGCC TATTTCATCA GCTTCTTCAT GCTCTGTGCC
    TTCCTGATCA TAAATCTCTT TGTGGCTGTG ATCATGGACA ACTTTGATTA TCTCACCAGA
    GATTGGTCCA TCCTGGGCCC CCATCACCTT GATGAATTCA AGAGGATCTG GTCTGAATAT
    GACCCTGGGG CCAAGGGCCG CATCAAACAC TTGGATGTGG TTGCCCTGCT GAGACGTATC
    CAGCCCCCTC TGGGATTTGG GAAGCTGTGC CCACACCGAG TGGCCTGCAA GAGACTTGTG
    GCAATGAACA TGCCCCTCAA CTCAGATGGG ACGGTGACAT TCAACGCCAC ACTCTTTGCC
    CTGGTCCGGA CATCCCTGAA GATCAAAACA GAAGGGAACC TGGAGCAAGC CAACCAGGAG
    CTGCGGATTG TCATCAAAAA GATCTGGAAG CGGATGAAAC AGAAGCTGCT AGATGAGGTC
    ATCCCCCCAC CAGACGAGGA GGAGGTCACC GTGGGCAAAT TCTACGCCAC ATTTCTGATC
    CAGGACTATT TCCGCAAATT CCGGCGGAGG AAAGAAAAAG GGCTACTAGG CAACGACGCC
    GCCCCTAGCA CCTCTTCCGC CCTTCAGGCT GGTCTGCGGA GCCTGCAGGA CTTGGGTCCT
    GAGATGCGGC AGGCCCTCAC CTGTGACACA GAGGAGGAGG AAGAAGAGGG GCAGGAGGGA
    GTGGAGGAGG AAGATGAAAA GGACTTGGAA ACTAACAAAG CCACGATGGT CTCCCAGCCC
    TCAGCTCGCC GGGGCTCCGG GATTTCTGTG TCTCTGCCTG TCGGGGACAG ACTTCCAGAT
    TCACTCTCCT TTGGGCCCAG TGATGATGAC AGGGGGACTC CCACCTCCAG TCAGCCCAGT
    GTGCCCCAGG CTGGATCCAA CACCCACAGG AGAGGCTCTG GGGCTCTCAT TTTCACCATC
    CCAGAAGAAG GAAATTCTCA GCCCAAGGGA ACCAAAGGGC AAAACAAGCA AGATGAGGAT
    GAGGAAGTCC CTGATCGAGC TCAGAGATAC ATGGATGGGC ACCTGGTACC ACGCCGCCGT
    CTGCTGCCCC CCACACCTGC AGGTCGGAAG CCCTCCTTCA CCATCCAGTG TCTGCAGCGC
    CAGGGCAGTT GTGAGGATTT ACCCATCCCA GGCACCTATC ATCGTGGGCG AAATTCAGGG
    CCCAATAGGG CTCAGGGTTC CTGGGCAACA CCACCTCAGC GGGGTCGGCT CCTGTATGCC
    CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC
    CCACTGCGCA CCTTCACCTG TCTGCACGTG CCTGGAACCC ACTCGGACCC CAGCCATGGG
    AAGAGGGGCA GTGCCGACAG CTTGGTGGAG GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC
    TTTGCTCGAG ACCCACGTTT CGTGGCCCTG GCCAAGCAGG AGATTGCAGA TGCGTGTCGC
    CTGACGCTGG ATGAGATGGA CAATGCTGCC AGTGACCTGC TGGCACAGGG AACCAGCTCT
    CTCTATAGCG ACGAGGAGTC CATCCTCTCC CGCTTCGATG AGGAGGACTT GGGAGACGAG
    ATGGCCTGCG TCCACGCCCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885325.1
    CDS86..3169
    Translation

    Target ORF information:

    RefSeq Version XM_017029836.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029836.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGACAGTGT TTGGGGCCTT CCTGCACCGC GGCTCCTTCT GCCGTAGCTG GTTTAATATG 
    TTGGATCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTTG GCATCCACTC CAGCGCCATC
    TCGGTGGTGA AGATTCTGCG AGTACTCCGA GTACTGCGGC CCCTCCGAGC CATCAACAGG
    GCCAAGGGAC TCAAGCATGT GGTGCAGTGT GTATTTGTGG CCATCCGGAC CATCGGAAAC
    ATCATGATTG TCACCACACT TCTGCAATTT ATGTTCGCCT GCATCGGGGT GCAGCTCTTC
    AAGGGGAAAT TCTACACCTG CACGGACGAG GCCAAACACA CCCCTCAAGA ATGCAAGGGC
    TCCTTCCTGG TATACCCAGA TGGAGACGTG TCACGGCCCC TGGTCCGGGA GCGGCTCTGG
    GTCAACAGTG ATTTCAACTT TGACAATGTC CTTTCAGCCA TGATGGCCCT GTTCACTGTC
    TCCACCTTTG AAGGCTGGCC TGCACTGCTA TACAAGGCCA TCGATGCATA TGCAGAGGAC
    CACGGCCCCA TCTATAATTA CCGTGTGGAG ATCTCAGTGT TCTTCATTGT CTACATCATC
    ATCATTGCGT TCTTCATGAT GAACATCTTC GTGGGCTTCG TCATCATCAC TTTCCGTGCC
    CAGGGCGAGC AGGAGTACCA AAACTGTGAG CTGGACAAGA ACCAGCGTCA ATGTGTGGAA
    TATGCCCTCA AGGCCCAGCC ACTCCGCCGT TACATCCCCA AGAACCCGCA TCAGTATCGT
    GTGTGGGCCA CTGTGAACTC TGCTGCCTTT GAGTACCTGA TGTTCCTGCT CATCCTGCTC
    AACACAGTTG CCCTAGCCAT GCAGCACTAT GAGCAGACTG CTCCCTTCAA CTATGCCATG
    GACATCCTCA ACATGGTCTT CACTGGCCTC TTCACTATTG AGATGGTGCT CAAAATCATC
    GCCTTCAAGC CCAAGCATTA CTTCACTGAT GCCTGGAACA CGTTTGACGC TCTTATTGTG
    GTGGGCAGCA TAGTGGATAT TGCCGTCACT GAAGTCAATA GCTCTGAGGA CAGCTCCCGC
    ATTTCCATTA CCTTCTTTCG CCTCTTCCGA GTTATGCGGC TGGTCAAGCT TCTCAGTAAG
    GGTGAAGGGA TCCGCACATT GCTCTGGACA TTCATCAAGT CCTTCCAGGC CTTGCCCTAT
    GTGGCTCTTC TCATCGCAAT GATATTCTTC ATCTATGCCG TCATTGGCAT GCAGATGTTC
    GGCAAGGTGG CTCTTCAGGA TGGCACACAG ATAAACCGAA ACAACAACTT CCAGACCTTT
    CCACAGGCTG TGCTGCTTCT GTTCAGGTGT GCCACTGGTG AGGCATGGCA GGAGATAATG
    CTTGCCAGCC TTCCCGGAAA TCGGTGTGAT CCTGAGTCTG ACTTCGGCCC TGGTGAAGAG
    TTTACCTGTG GTAGCAATTT TGCCATCGCC TATTTCATCA GCTTCTTCAT GCTCTGTGCC
    TTCCTGATCA TAAATCTCTT TGTGGCTGTG ATCATGGACA ACTTTGATTA TCTCACCAGA
    GATTGGTCCA TCCTGGGCCC CCATCACCTT GATGAATTCA AGAGGATCTG GTCTGAATAT
    GACCCTGGGG CCAAGGGCCG CATCAAACAC TTGGATGTGG TTGCCCTGCT GAGACGTATC
    CAGCCCCCTC TGGGATTTGG GAAGCTGTGC CCACACCGAG TGGCCTGCAA GAGACTTGTG
    GCAATGAACA TGCCCCTCAA CTCAGATGGG ACGGTGACAT TCAACGCCAC ACTCTTTGCC
    CTGGTCCGGA CATCCCTGAA GATCAAAACA GAAGGGAACC TGGAGCAAGC CAACCAGGAG
    CTGCGGATTG TCATCAAAAA GATCTGGAAG CGGATGAAAC AGAAGCTGCT AGATGAGGTC
    ATCCCCCCAC CAGACGAGGA GGAGGTCACC GTGGGCAAAT TCTACGCCAC ATTTCTGATC
    CAGGACTATT TCCGCAAATT CCGGCGGAGG AAAGAAAAAG GGCTACTAGG CAACGACGCC
    GCCCCTAGCA CCTCTTCCGC CCTTCAGGCT GGTCTGCGGA GCCTGCAGGA CTTGGGTCCT
    GAGATGCGGC AGGCCCTCAC CTGTGACACA GAGGAGGAGG AAGAAGAGGG GCAGGAGGGA
    GTGGAGGAGG AAGATGAAAA GGACTTGGAA ACTAACAAAG CCACGATGGT CTCCCAGCCC
    TCAGCTCGCC GGGGCTCCGG GATTTCTGTG TCTCTGCCTG TCGGGGACAG ACTTCCAGAT
    TCACTCTCCT TTGGGCCCAG TGATGATGAC AGGGGGACTC CCACCTCCAG TCAGCCCAGT
    GTGCCCCAGG CTGGATCCAA CACCCACAGG AGAGGCTCTG GGGCTCTCAT TTTCACCATC
    CCAGAAGAAG GAAATTCTCA GCCCAAGGGA ACCAAAGGGC AAAACAAGCA AGATGAGGAT
    GAGGAAGTCC CTGATCGAGC TCAGAGATAC ATGGATGGGC ACCTGGTACC ACGCCGCCGT
    CTGCTGCCCC CCACACCTGC AGGTCGGAAG CCCTCCTTCA CCATCCAGTG TCTGCAGCGC
    CAGGGCAGTT GTGAGGATTT ACCCATCCCA GGCACCTATC ATCGTGGGCG AAATTCAGGG
    CCCAATAGGG CTCAGGGTTC CTGGGCAACA CCACCTCAGC GGGGTCGGCT CCTGTATGCC
    CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC
    CCACTGCGCA CCTTCACCTG TCTGCACGTG CCTGGAACCC ACTCGGACCC CAGCCATGGG
    AAGAGGGGCA GTGCCGACAG CTTGGTGGAG GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC
    TTTGCTCGAG ACCCACGTTT CGTGGCCCTG GCCAAGCAGG AGATTGCAGA TGCGTGTCGC
    CTGACGCTGG ATGAGATGGA CAATGCTGCC AGTGACCTGC TGGCACAGGG AACCAGCTCT
    CTCTATAGCG ACGAGGAGTC CATCCTCTCC CGCTTCGATG AGGAGGACTT GGGAGACGAG
    ATGGCCTGCG TCCACGCCCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21327
    Clone ID Related Accession (Same CDS sequence) NM_001256790.2 , NM_001256790.3
    Accession Version NM_001256790.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF201304.1, AF067227.1 and AC232271.2. On Aug 21, 2013 this sequence version replaced NM_001256790.1. Transcript Variant: This variant (3) uses two alternate splice sites in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF067227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
    GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC
    TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC
    AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG
    ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA
    CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA
    CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG
    AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA
    GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC
    TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG
    ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT
    TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG
    CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG
    TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT
    GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC
    CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC
    GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG
    GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT
    GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT
    AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC
    AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG
    GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC
    AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA
    GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC
    ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC
    AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC
    TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC
    CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA
    TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG
    GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC
    TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC
    CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT
    CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT
    GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC
    AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT
    GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG
    AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA
    GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG
    GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC
    CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC
    ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT
    GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT
    TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG
    TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG
    AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA
    CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT
    GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA
    TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG
    GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT
    GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT
    GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC
    ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG
    TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG
    CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC
    AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC
    ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT
    GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC
    AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG
    CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC
    ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AATGGTGGCC ACCTTGGCGA GAGCTCTGAG
    GACAGCTCCC GCATTTCCAT TACCTTCTTT CGCCTCTTCC GAGTTATGCG GCTGGTCAAG
    CTTCTCAGTA AGGGTGAAGG GATCCGCACA TTGCTCTGGA CATTCATCAA GTCCTTCCAG
    GCCTTGCCCT ATGTGGCTCT TCTCATCGCA ATGATATTCT TCATCTATGC CGTCATTGGC
    ATGCAGATGT TCGGCAAGGT GGCTCTTCAG GATGGCACAC AGATAAACCG AAACAACAAC
    TTCCAGACCT TTCCACAGGC TGTGCTGCTT CTGTTCAGGT GTGCCACTGG TGAGGCATGG
    CAGGAGATAA TGCTTGCCAG CCTTCCCGGA AATCGGTGTG ATCCTGAGTC TGACTTCGGC
    CCTGGTGAAG AGTTTACCTG TGGTAGCAAT TTTGCCATCG CCTATTTCAT CAGCTTCTTC
    ATGCTCTGTG CCTTCCTGAT CATAAATCTC TTTGTGGCTG TGATCATGGA CAACTTTGAT
    TATCTCACCA GAGATTGGTC CATCCTGGGC CCCCATCACC TTGATGAATT CAAGAGGATC
    TGGTCTGAAT ATGACCCTGG GGCCAAGGGC CGCATCAAAC ACTTGGATGT GGTTGCCCTG
    CTGAGACGTA TCCAGCCCCC TCTGGGATTT GGGAAGCTGT GCCCACACCG AGTGGCCTGC
    AAGAGACTTG TGGCAATGAA CATGCCCCTC AACTCAGATG GGACGGTGAC ATTCAACGCC
    ACACTCTTTG CCCTGGTCCG GACATCCCTG AAGATCAAAA CAGAAGGGAA CCTGGAGCAA
    GCCAACCAGG AGCTGCGGAT TGTCATCAAA AAGATCTGGA AGCGGATGAA ACAGAAGCTG
    CTAGATGAGG TCATCCCCCC ACCAGACGAG GAGGAGGTCA CCGTGGGCAA ATTCTACGCC
    ACATTTCTGA TCCAGGACTA TTTCCGCAAA TTCCGGCGGA GGAAAGAAAA AGGGCTACTA
    GGCAACGACG CCGCCCCTAG CACCTCTTCC GCCCTTCAGG CTGGTCTGCG GAGCCTGCAG
    GACTTGGGTC CTGAGATGCG GCAGGCCCTC ACCTGTGACA CAGAGGAGGA GGAAGAAGAG
    GGGCAGGAGG GAGTGGAGGA GGAAGATGAA AAGGACTTGG AAACTAACAA AGCCACGATG
    GTCTCCCAGC CCTCAGCTCG CCGGGGCTCC GGGATTTCTG TGTCTCTGCC TGTCGGGGAC
    AGACTTCCAG ATTCACTCTC CTTTGGGCCC AGTGATGATG ACAGGGGGAC TCCCACCTCC
    AGTCAGCCCA GTGTGCCCCA GGCTGGATCC AACACCCACA GGAGAGGCTC TGGGGCTCTC
    ATTTTCACCA TCCCAGAAGA AGGAAATTCT CAGCCCAAGG GAACCAAAGG GCAAAACAAG
    CAAGATGAGG ATGAGGAAGT CCCTGATCGG CTTTCCTACC TAGATGAGCA GGCAGGGACT
    CCCCCGTGCT CAGTCCTTTT GCCACCTCAC AGAGCTCAGA GATACATGGA TGGGCACCTG
    GTACCACGCC GCCGTCTGCT GCCCCCCACA CCTGCAGGTC GGAAGCCCTC CTTCACCATC
    CAGTGTCTGC AGCGCCAGGG CAGTTGTGAG GATTTACCCA TCCCAGGCAC CTATCATCGT
    GGGCGAAATT CAGGGCCCAA TAGGGCTCAG GGTTCCTGGG CAACACCACC TCAGCGGGGT
    CGGCTCCTGT ATGCCCCGCT GTTGTTGGTG GAAGAGGGCG CAGCGGGGGA GGGGTACCTC
    GGCAGATCCA GTGGCCCACT GCGCACCTTC ACCTGTCTGC ACGTGCCTGG AACCCACTCG
    GACCCCAGCC ATGGGAAGAG GGGCAGTGCC GACAGCTTGG TGGAGGCTGT GCTTATCTCA
    GAGGGTCTGG GCCTCTTTGC TCGAGACCCA CGTTTCGTGG CCCTGGCCAA GCAGGAGATT
    GCAGATGCGT GTCGCCTGAC GCTGGATGAG ATGGACAATG CTGCCAGTGA CCTGCTGGCA
    CAGGGAACCA GCTCTCTCTA TAGCGACGAG GAGTCCATCC TCTCCCGCTT CGATGAGGAG
    GACTTGGGAG ACGAGATGGC CTGCGTCCAC GCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243719.1
    CDS63..5801
    Translation

    Target ORF information:

    RefSeq Version NM_001256790.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256790.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
    GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC
    TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC
    AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG
    ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA
    CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA
    CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG
    AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA
    GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC
    TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG
    ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT
    TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG
    CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG
    TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT
    GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC
    CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC
    GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG
    GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT
    GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT
    AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC
    AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG
    GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC
    AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA
    GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC
    ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC
    AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC
    TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC
    CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA
    TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG
    GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC
    TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC
    CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT
    CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT
    GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC
    AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT
    GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG
    AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA
    GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG
    GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC
    CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC
    ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT
    GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT
    TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG
    TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG
    AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA
    CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT
    GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA
    TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG
    GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT
    GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT
    GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC
    ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG
    TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG
    CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC
    AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC
    ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT
    GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC
    AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG
    CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC
    ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AATGGTGGCC ACCTTGGCGA GAGCTCTGAG
    GACAGCTCCC GCATTTCCAT TACCTTCTTT CGCCTCTTCC GAGTTATGCG GCTGGTCAAG
    CTTCTCAGTA AGGGTGAAGG GATCCGCACA TTGCTCTGGA CATTCATCAA GTCCTTCCAG
    GCCTTGCCCT ATGTGGCTCT TCTCATCGCA ATGATATTCT TCATCTATGC CGTCATTGGC
    ATGCAGATGT TCGGCAAGGT GGCTCTTCAG GATGGCACAC AGATAAACCG AAACAACAAC
    TTCCAGACCT TTCCACAGGC TGTGCTGCTT CTGTTCAGGT GTGCCACTGG TGAGGCATGG
    CAGGAGATAA TGCTTGCCAG CCTTCCCGGA AATCGGTGTG ATCCTGAGTC TGACTTCGGC
    CCTGGTGAAG AGTTTACCTG TGGTAGCAAT TTTGCCATCG CCTATTTCAT CAGCTTCTTC
    ATGCTCTGTG CCTTCCTGAT CATAAATCTC TTTGTGGCTG TGATCATGGA CAACTTTGAT
    TATCTCACCA GAGATTGGTC CATCCTGGGC CCCCATCACC TTGATGAATT CAAGAGGATC
    TGGTCTGAAT ATGACCCTGG GGCCAAGGGC CGCATCAAAC ACTTGGATGT GGTTGCCCTG
    CTGAGACGTA TCCAGCCCCC TCTGGGATTT GGGAAGCTGT GCCCACACCG AGTGGCCTGC
    AAGAGACTTG TGGCAATGAA CATGCCCCTC AACTCAGATG GGACGGTGAC ATTCAACGCC
    ACACTCTTTG CCCTGGTCCG GACATCCCTG AAGATCAAAA CAGAAGGGAA CCTGGAGCAA
    GCCAACCAGG AGCTGCGGAT TGTCATCAAA AAGATCTGGA AGCGGATGAA ACAGAAGCTG
    CTAGATGAGG TCATCCCCCC ACCAGACGAG GAGGAGGTCA CCGTGGGCAA ATTCTACGCC
    ACATTTCTGA TCCAGGACTA TTTCCGCAAA TTCCGGCGGA GGAAAGAAAA AGGGCTACTA
    GGCAACGACG CCGCCCCTAG CACCTCTTCC GCCCTTCAGG CTGGTCTGCG GAGCCTGCAG
    GACTTGGGTC CTGAGATGCG GCAGGCCCTC ACCTGTGACA CAGAGGAGGA GGAAGAAGAG
    GGGCAGGAGG GAGTGGAGGA GGAAGATGAA AAGGACTTGG AAACTAACAA AGCCACGATG
    GTCTCCCAGC CCTCAGCTCG CCGGGGCTCC GGGATTTCTG TGTCTCTGCC TGTCGGGGAC
    AGACTTCCAG ATTCACTCTC CTTTGGGCCC AGTGATGATG ACAGGGGGAC TCCCACCTCC
    AGTCAGCCCA GTGTGCCCCA GGCTGGATCC AACACCCACA GGAGAGGCTC TGGGGCTCTC
    ATTTTCACCA TCCCAGAAGA AGGAAATTCT CAGCCCAAGG GAACCAAAGG GCAAAACAAG
    CAAGATGAGG ATGAGGAAGT CCCTGATCGG CTTTCCTACC TAGATGAGCA GGCAGGGACT
    CCCCCGTGCT CAGTCCTTTT GCCACCTCAC AGAGCTCAGA GATACATGGA TGGGCACCTG
    GTACCACGCC GCCGTCTGCT GCCCCCCACA CCTGCAGGTC GGAAGCCCTC CTTCACCATC
    CAGTGTCTGC AGCGCCAGGG CAGTTGTGAG GATTTACCCA TCCCAGGCAC CTATCATCGT
    GGGCGAAATT CAGGGCCCAA TAGGGCTCAG GGTTCCTGGG CAACACCACC TCAGCGGGGT
    CGGCTCCTGT ATGCCCCGCT GTTGTTGGTG GAAGAGGGCG CAGCGGGGGA GGGGTACCTC
    GGCAGATCCA GTGGCCCACT GCGCACCTTC ACCTGTCTGC ACGTGCCTGG AACCCACTCG
    GACCCCAGCC ATGGGAAGAG GGGCAGTGCC GACAGCTTGG TGGAGGCTGT GCTTATCTCA
    GAGGGTCTGG GCCTCTTTGC TCGAGACCCA CGTTTCGTGG CCCTGGCCAA GCAGGAGATT
    GCAGATGCGT GTCGCCTGAC GCTGGATGAG ATGGACAATG CTGCCAGTGA CCTGCTGGCA
    CAGGGAACCA GCTCTCTCTA TAGCGACGAG GAGTCCATCC TCTCCCGCTT CGATGAGGAG
    GACTTGGGAG ACGAGATGGC CTGCGTCCAC GCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21327
    Clone ID Related Accession (Same CDS sequence) NM_001256790.2 , NM_001256790.3
    Accession Version NM_001256790.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF201304.1, AF067227.1 and AC232271.2. On Jun 2, 2019 this sequence version replaced NM_001256790.2. Transcript Variant: This variant (3) uses two alternate splice sites in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF067227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
    GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC
    TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC
    AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG
    ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA
    CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA
    CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG
    AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA
    GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC
    TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG
    ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT
    TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG
    CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG
    TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT
    GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC
    CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC
    GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG
    GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT
    GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT
    AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC
    AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG
    GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC
    AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA
    GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC
    ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC
    AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC
    TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC
    CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA
    TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG
    GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC
    TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC
    CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT
    CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT
    GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC
    AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT
    GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG
    AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA
    GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG
    GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC
    CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC
    ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT
    GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT
    TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG
    TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG
    AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA
    CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT
    GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA
    TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG
    GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT
    GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT
    GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC
    ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG
    TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG
    CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC
    AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC
    ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT
    GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC
    AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG
    CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC
    ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AATGGTGGCC ACCTTGGCGA GAGCTCTGAG
    GACAGCTCCC GCATTTCCAT TACCTTCTTT CGCCTCTTCC GAGTTATGCG GCTGGTCAAG
    CTTCTCAGTA AGGGTGAAGG GATCCGCACA TTGCTCTGGA CATTCATCAA GTCCTTCCAG
    GCCTTGCCCT ATGTGGCTCT TCTCATCGCA ATGATATTCT TCATCTATGC CGTCATTGGC
    ATGCAGATGT TCGGCAAGGT GGCTCTTCAG GATGGCACAC AGATAAACCG AAACAACAAC
    TTCCAGACCT TTCCACAGGC TGTGCTGCTT CTGTTCAGGT GTGCCACTGG TGAGGCATGG
    CAGGAGATAA TGCTTGCCAG CCTTCCCGGA AATCGGTGTG ATCCTGAGTC TGACTTCGGC
    CCTGGTGAAG AGTTTACCTG TGGTAGCAAT TTTGCCATCG CCTATTTCAT CAGCTTCTTC
    ATGCTCTGTG CCTTCCTGAT CATAAATCTC TTTGTGGCTG TGATCATGGA CAACTTTGAT
    TATCTCACCA GAGATTGGTC CATCCTGGGC CCCCATCACC TTGATGAATT CAAGAGGATC
    TGGTCTGAAT ATGACCCTGG GGCCAAGGGC CGCATCAAAC ACTTGGATGT GGTTGCCCTG
    CTGAGACGTA TCCAGCCCCC TCTGGGATTT GGGAAGCTGT GCCCACACCG AGTGGCCTGC
    AAGAGACTTG TGGCAATGAA CATGCCCCTC AACTCAGATG GGACGGTGAC ATTCAACGCC
    ACACTCTTTG CCCTGGTCCG GACATCCCTG AAGATCAAAA CAGAAGGGAA CCTGGAGCAA
    GCCAACCAGG AGCTGCGGAT TGTCATCAAA AAGATCTGGA AGCGGATGAA ACAGAAGCTG
    CTAGATGAGG TCATCCCCCC ACCAGACGAG GAGGAGGTCA CCGTGGGCAA ATTCTACGCC
    ACATTTCTGA TCCAGGACTA TTTCCGCAAA TTCCGGCGGA GGAAAGAAAA AGGGCTACTA
    GGCAACGACG CCGCCCCTAG CACCTCTTCC GCCCTTCAGG CTGGTCTGCG GAGCCTGCAG
    GACTTGGGTC CTGAGATGCG GCAGGCCCTC ACCTGTGACA CAGAGGAGGA GGAAGAAGAG
    GGGCAGGAGG GAGTGGAGGA GGAAGATGAA AAGGACTTGG AAACTAACAA AGCCACGATG
    GTCTCCCAGC CCTCAGCTCG CCGGGGCTCC GGGATTTCTG TGTCTCTGCC TGTCGGGGAC
    AGACTTCCAG ATTCACTCTC CTTTGGGCCC AGTGATGATG ACAGGGGGAC TCCCACCTCC
    AGTCAGCCCA GTGTGCCCCA GGCTGGATCC AACACCCACA GGAGAGGCTC TGGGGCTCTC
    ATTTTCACCA TCCCAGAAGA AGGAAATTCT CAGCCCAAGG GAACCAAAGG GCAAAACAAG
    CAAGATGAGG ATGAGGAAGT CCCTGATCGG CTTTCCTACC TAGATGAGCA GGCAGGGACT
    CCCCCGTGCT CAGTCCTTTT GCCACCTCAC AGAGCTCAGA GATACATGGA TGGGCACCTG
    GTACCACGCC GCCGTCTGCT GCCCCCCACA CCTGCAGGTC GGAAGCCCTC CTTCACCATC
    CAGTGTCTGC AGCGCCAGGG CAGTTGTGAG GATTTACCCA TCCCAGGCAC CTATCATCGT
    GGGCGAAATT CAGGGCCCAA TAGGGCTCAG GGTTCCTGGG CAACACCACC TCAGCGGGGT
    CGGCTCCTGT ATGCCCCGCT GTTGTTGGTG GAAGAGGGCG CAGCGGGGGA GGGGTACCTC
    GGCAGATCCA GTGGCCCACT GCGCACCTTC ACCTGTCTGC ACGTGCCTGG AACCCACTCG
    GACCCCAGCC ATGGGAAGAG GGGCAGTGCC GACAGCTTGG TGGAGGCTGT GCTTATCTCA
    GAGGGTCTGG GCCTCTTTGC TCGAGACCCA CGTTTCGTGG CCCTGGCCAA GCAGGAGATT
    GCAGATGCGT GTCGCCTGAC GCTGGATGAG ATGGACAATG CTGCCAGTGA CCTGCTGGCA
    CAGGGAACCA GCTCTCTCTA TAGCGACGAG GAGTCCATCC TCTCCCGCTT CGATGAGGAG
    GACTTGGGAG ACGAGATGGC CTGCGTCCAC GCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243719.1
    CDS32..5770
    Translation

    Target ORF information:

    RefSeq Version NM_001256790.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256790.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
    GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC
    TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC
    AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG
    ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA
    CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA
    CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG
    AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA
    GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC
    TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG
    ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT
    TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG
    CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG
    TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT
    GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC
    CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC
    GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG
    GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT
    GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT
    AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC
    AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG
    GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC
    AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA
    GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC
    ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC
    AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC
    TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC
    CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA
    TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG
    GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC
    TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC
    CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT
    CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT
    GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC
    AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT
    GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG
    AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA
    GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG
    GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC
    CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC
    ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT
    GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT
    TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG
    TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG
    AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA
    CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT
    GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA
    TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG
    GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT
    GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT
    GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC
    ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG
    TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG
    CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC
    AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC
    ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT
    GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC
    AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG
    CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC
    ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AATGGTGGCC ACCTTGGCGA GAGCTCTGAG
    GACAGCTCCC GCATTTCCAT TACCTTCTTT CGCCTCTTCC GAGTTATGCG GCTGGTCAAG
    CTTCTCAGTA AGGGTGAAGG GATCCGCACA TTGCTCTGGA CATTCATCAA GTCCTTCCAG
    GCCTTGCCCT ATGTGGCTCT TCTCATCGCA ATGATATTCT TCATCTATGC CGTCATTGGC
    ATGCAGATGT TCGGCAAGGT GGCTCTTCAG GATGGCACAC AGATAAACCG AAACAACAAC
    TTCCAGACCT TTCCACAGGC TGTGCTGCTT CTGTTCAGGT GTGCCACTGG TGAGGCATGG
    CAGGAGATAA TGCTTGCCAG CCTTCCCGGA AATCGGTGTG ATCCTGAGTC TGACTTCGGC
    CCTGGTGAAG AGTTTACCTG TGGTAGCAAT TTTGCCATCG CCTATTTCAT CAGCTTCTTC
    ATGCTCTGTG CCTTCCTGAT CATAAATCTC TTTGTGGCTG TGATCATGGA CAACTTTGAT
    TATCTCACCA GAGATTGGTC CATCCTGGGC CCCCATCACC TTGATGAATT CAAGAGGATC
    TGGTCTGAAT ATGACCCTGG GGCCAAGGGC CGCATCAAAC ACTTGGATGT GGTTGCCCTG
    CTGAGACGTA TCCAGCCCCC TCTGGGATTT GGGAAGCTGT GCCCACACCG AGTGGCCTGC
    AAGAGACTTG TGGCAATGAA CATGCCCCTC AACTCAGATG GGACGGTGAC ATTCAACGCC
    ACACTCTTTG CCCTGGTCCG GACATCCCTG AAGATCAAAA CAGAAGGGAA CCTGGAGCAA
    GCCAACCAGG AGCTGCGGAT TGTCATCAAA AAGATCTGGA AGCGGATGAA ACAGAAGCTG
    CTAGATGAGG TCATCCCCCC ACCAGACGAG GAGGAGGTCA CCGTGGGCAA ATTCTACGCC
    ACATTTCTGA TCCAGGACTA TTTCCGCAAA TTCCGGCGGA GGAAAGAAAA AGGGCTACTA
    GGCAACGACG CCGCCCCTAG CACCTCTTCC GCCCTTCAGG CTGGTCTGCG GAGCCTGCAG
    GACTTGGGTC CTGAGATGCG GCAGGCCCTC ACCTGTGACA CAGAGGAGGA GGAAGAAGAG
    GGGCAGGAGG GAGTGGAGGA GGAAGATGAA AAGGACTTGG AAACTAACAA AGCCACGATG
    GTCTCCCAGC CCTCAGCTCG CCGGGGCTCC GGGATTTCTG TGTCTCTGCC TGTCGGGGAC
    AGACTTCCAG ATTCACTCTC CTTTGGGCCC AGTGATGATG ACAGGGGGAC TCCCACCTCC
    AGTCAGCCCA GTGTGCCCCA GGCTGGATCC AACACCCACA GGAGAGGCTC TGGGGCTCTC
    ATTTTCACCA TCCCAGAAGA AGGAAATTCT CAGCCCAAGG GAACCAAAGG GCAAAACAAG
    CAAGATGAGG ATGAGGAAGT CCCTGATCGG CTTTCCTACC TAGATGAGCA GGCAGGGACT
    CCCCCGTGCT CAGTCCTTTT GCCACCTCAC AGAGCTCAGA GATACATGGA TGGGCACCTG
    GTACCACGCC GCCGTCTGCT GCCCCCCACA CCTGCAGGTC GGAAGCCCTC CTTCACCATC
    CAGTGTCTGC AGCGCCAGGG CAGTTGTGAG GATTTACCCA TCCCAGGCAC CTATCATCGT
    GGGCGAAATT CAGGGCCCAA TAGGGCTCAG GGTTCCTGGG CAACACCACC TCAGCGGGGT
    CGGCTCCTGT ATGCCCCGCT GTTGTTGGTG GAAGAGGGCG CAGCGGGGGA GGGGTACCTC
    GGCAGATCCA GTGGCCCACT GCGCACCTTC ACCTGTCTGC ACGTGCCTGG AACCCACTCG
    GACCCCAGCC ATGGGAAGAG GGGCAGTGCC GACAGCTTGG TGGAGGCTGT GCTTATCTCA
    GAGGGTCTGG GCCTCTTTGC TCGAGACCCA CGTTTCGTGG CCCTGGCCAA GCAGGAGATT
    GCAGATGCGT GTCGCCTGAC GCTGGATGAG ATGGACAATG CTGCCAGTGA CCTGCTGGCA
    CAGGGAACCA GCTCTCTCTA TAGCGACGAG GAGTCCATCC TCTCCCGCTT CGATGAGGAG
    GACTTGGGAG ACGAGATGGC CTGCGTCCAC GCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21801
    Clone ID Related Accession (Same CDS sequence) NM_001256789.3 , NM_001256789.2
    Accession Version NM_001256789.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5901bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ224874.1, AC232271.2 and AF201304.1. On Jun 2, 2019 this sequence version replaced NM_001256789.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ224874.1, JF701915.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
    GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT
    GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG
    GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT
    CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG
    ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG
    GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC
    GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC
    CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT
    GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT
    GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA
    TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC
    GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG
    CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA
    GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC
    TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG
    AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC
    CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC
    GCCGATGACA ACCTTGGGCC ACAGCTGGCC GAGCTGACCA ATAGGAGGCG TGGACGTCTG
    CGCTGGTTCA GTCATTCTAC TCGCTCCACA CACTCCACCA GCAGCCATGC CAGCCTCCCA
    GCCAGTGACA CCGGTTCCAT GACAGAGACC CAAGGCGATG AGGATGAGGA GGAGGGGGCT
    CTGGCCAGCT GTACACGCTG CCTAAACAAG ATCATGAAAA CCAGAGTCTG CCGCCGCCTC
    CGCCGAGCCA ACCGGGTCCT TCGGGCACGC TGCCGTCGGG CAGTGAAGTC CAATGCCTGC
    TACTGGGCTG TGCTGTTGCT CGTCTTCCTC AACACGTTGA CCATCGCCTC TGAGCACCAC
    GGGCAGCCTG TGTGGCTCAC CCAGATCCAG GAGTATGCCA ACAAAGTGTT GCTCTGTCTG
    TTCACGGTGG AGATGCTTCT CAAATTGTAC GGTCTGGGCC CCTCTGCCTA TGTGTCTTCC
    TTCTTCAACC GCTTTGACTG CTTTGTGGTC TGTGGGGGCA TCCTAGAGAC CACCTTGGTG
    GAGGTGGGTG CCATGCAGCC CTTGGGCATC TCAGTGCTCC GATGTGTGCG CCTCCTCAGG
    ATCTTTAAGG TCACCAGACA CTGGGCTTCT CTGAGCAATC TGGTGGCATC CCTGCTCAAT
    TCAATGAAAT CCATCGCATC CTTGCTGCTT CTCCTCTTCC TCTTCATCAT TATCTTCTCC
    CTGCTTGGCA TGCAGCTGTT TGGGGGCAAG TTCAACTTTG ACCAGACCCA CACCAAGCGA
    AGCACCTTTG ACACGTTCCC CCAGGCCCTC CTCACTGTCT TTCAGATCCT GACAGGTGAG
    GACTGGAACG TGGTCATGTA TGATGGTATC ATGGCATATG GTGGCCCCTT CTTCCCAGGA
    ATGTTGGTGT GCATCTATTT CATCATTCTC TTCATCTGTG GCAACTACAT CCTGTTGAAC
    GTGTTTCTTG CCATTGCTGT GGACAACCTG GCCAGTGGAG ATGCAGGCAC TGCCAAGGAC
    AAGGGCGGGG AGAAGAGCAA TGAGAAGGAT CTCCCACAGG AGAATGAAGG CCTGGTGCCT
    GGTGTGGAGA AAGAGGAAGA GGAGGGTGCA AGGAGGGAAG GAGCAGACAT GGAGGAGGAG
    GAGGAGGAGG AAGAAGAGGA AGAAGAGGAA GAAGAGGAAG AGGGTGCAGG GGGTGTGGAA
    CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAGGG CAGCGCCTTC
    TTCTGCCTCA GCCAAACCAA CCCGCTGAGG AAGGGCTGCC ACACCCTCAT CCACCATCAT
    GTCTTCACCA ATCTTATCCT GGTGTTCATC ATCCTCAGCA GTGTGTCCCT GGCCGCTGAG
    GACCCCATCC GAGCCCACTC CTTCCGCAAC CATATTCTGG GTTACTTCGA TTATGCCTTC
    ACCTCCATTT TCACTGTGGA GATTCTACTA AAGATGACAG TGTTTGGGGC CTTCCTGCAC
    CGCGGCTCCT TCTGCCGTAG CTGGTTTAAT ATGTTGGATC TGCTGGTGGT CAGTGTGTCC
    CTCATCTCCT TTGGCATCCA CTCCAGCGCC ATCTCGGTGG TGAAGATTCT GCGAGTACTC
    CGAGTACTGC GGCCCCTCCG AGCCATCAAC AGGGCCAAGG GACTCAAGCA TGTGGTGCAG
    TGTGTATTTG TGGCCATCCG GACCATCGGA AACATCATGA TTGTCACCAC ACTTCTGCAA
    TTTATGTTCG CCTGCATCGG GGTGCAGCTC TTCAAGGGGA AATTCTACAC CTGCACGGAC
    GAGGCCAAAC ACACCCCTCA AGAATGCAAG GGCTCCTTCC TGGTATACCC AGATGGAGAC
    GTGTCACGGC CCCTGGTCCG GGAGCGGCTC TGGGTCAACA GTGATTTCAA CTTTGACAAT
    GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCCACCT TTGAAGGCTG GCCTGCACTG
    CTATACAAGG CCATCGATGC ATATGCAGAG GACCACGGCC CCATCTATAA TTACCGTGTG
    GAGATCTCAG TGTTCTTCAT TGTCTACATC ATCATCATTG CGTTCTTCAT GATGAACATC
    TTCGTGGGCT TCGTCATCAT CACTTTCCGT GCCCAGGGCG AGCAGGAGTA CCAAAACTGT
    GAGCTGGACA AGAACCAGCG TCAATGTGTG GAATATGCCC TCAAGGCCCA GCCACTCCGC
    CGTTACATCC CCAAGAACCC GCATCAGTAT CGTGTGTGGG CCACTGTGAA CTCTGCTGCC
    TTTGAGTACC TGATGTTCCT GCTCATCCTG CTCAACACAG TTGCCCTAGC CATGCAGCAC
    TATGAGCAGA CTGCTCCCTT CAACTATGCC ATGGACATCC TCAACATGGT CTTCACTGGC
    CTCTTCACTA TTGAGATGGT GCTCAAAATC ATCGCCTTCA AGCCCAAGCA TTACTTCACT
    GATGCCTGGA ACACGTTTGA CGCTCTTATT GTGGTGGGCA GCATAGTGGA TATTGCCGTC
    ACTGAAGTCA ATAATGGTGG CCACCTTGGC GAGAGCTCTG AGGACAGCTC CCGCATTTCC
    ATTACCTTCT TTCGCCTCTT CCGAGTTATG CGGCTGGTCA AGCTTCTCAG TAAGGGTGAA
    GGGATCCGCA CATTGCTCTG GACATTCATC AAGTCCTTCC AGGCCTTGCC CTATGTGGCT
    CTTCTCATCG CAATGATATT CTTCATCTAT GCCGTCATTG GCATGCAGAT GTTCGGCAAG
    GTGGCTCTTC AGGATGGCAC ACAGATAAAC CGAAACAACA ACTTCCAGAC CTTTCCACAG
    GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAGGAGAT AATGCTTGCC
    AGCCTTCCCG GAAATCGGTG TGATCCTGAG TCTGACTTCG GCCCTGGTGA AGAGTTTACC
    TGTGGTAGCA ATTTTGCCAT CGCCTATTTC ATCAGCTTCT TCATGCTCTG TGCCTTCCTG
    ATCATAAATC TCTTTGTGGC TGTGATCATG GACAACTTTG ATTATCTCAC CAGAGATTGG
    TCCATCCTGG GCCCCCATCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCT
    GGGGCCAAGG GCCGCATCAA ACACTTGGAT GTGGTTGCCC TGCTGAGACG TATCCAGCCC
    CCTCTGGGAT TTGGGAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT TGTGGCAATG
    AACATGCCCC TCAACTCAGA TGGGACGGTG ACATTCAACG CCACACTCTT TGCCCTGGTC
    CGGACATCCC TGAAGATCAA AACAGAAGGG AACCTGGAGC AAGCCAACCA GGAGCTGCGG
    ATTGTCATCA AAAAGATCTG GAAGCGGATG AAACAGAAGC TGCTAGATGA GGTCATCCCC
    CCACCAGACG AGGAGGAGGT CACCGTGGGC AAATTCTACG CCACATTTCT GATCCAGGAC
    TATTTCCGCA AATTCCGGCG GAGGAAAGAA AAAGGGCTAC TAGGCAACGA CGCCGCCCCT
    AGCACCTCTT CCGCCCTTCA GGCTGGTCTG CGGAGCCTGC AGGACTTGGG TCCTGAGATG
    CGGCAGGCCC TCACCTGTGA CACAGAGGAG GAGGAAGAAG AGGGGCAGGA GGGAGTGGAG
    GAGGAAGATG AAAAGGACTT GGAAACTAAC AAAGCCACGA TGGTCTCCCA GCCCTCAGCT
    CGCCGGGGCT CCGGGATTTC TGTGTCTCTG CCTGTCGGGG ACAGACTTCC AGATTCACTC
    TCCTTTGGGC CCAGTGATGA TGACAGGGGG ACTCCCACCT CCAGTCAGCC CAGTGTGCCC
    CAGGCTGGAT CCAACACCCA CAGGAGAGGC TCTGGGGCTC TCATTTTCAC CATCCCAGAA
    GAAGGAAATT CTCAGCCCAA GGGAACCAAA GGGCAAAACA AGCAAGATGA GGATGAGGAA
    GTCCCTGATC GGCTTTCCTA CCTAGATGAG CAGGCAGGGA CTCCCCCGTG CTCAGTCCTT
    TTGCCACCTC ACAGAGCTCA GAGATACATG GATGGGCACC TGGTACCACG CCGCCGTCTG
    CTGCCCCCCA CACCTGCAGG TCGGAAGCCC TCCTTCACCA TCCAGTGTCT GCAGCGCCAG
    GGCAGTTGTG AGGATTTACC CATCCCAGGC ACCTATCATC GTGGGCGAAA TTCAGGGCCC
    AATAGGGCTC AGGGTTCCTG GGCAACACCA CCTCAGCGGG GTCGGCTCCT GTATGCCCCG
    CTGTTGTTGG TGGAAGAGGG CGCAGCGGGG GAGGGGTACC TCGGCAGATC CAGTGGCCCA
    CTGCGCACCT TCACCTGTCT GCACGTGCCT GGAACCCACT CGGACCCCAG CCATGGGAAG
    AGGGGCAGTG CCGACAGCTT GGTGGAGGCT GTGCTTATCT CAGAGGGTCT GGGCCTCTTT
    GCTCGAGACC CACGTTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC GTGTCGCCTG
    ACGCTGGATG AGATGGACAA TGCTGCCAGT GACCTGCTGG CACAGGGAAC CAGCTCTCTC
    TATAGCGACG AGGAGTCCAT CCTCTCCCGC TTCGATGAGG AGGACTTGGG AGACGAGATG
    GCCTGCGTCC ACGCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243718.1
    CDS32..5932
    Translation

    Target ORF information:

    RefSeq Version NM_001256789.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256789.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
    GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT
    GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG
    GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT
    CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG
    ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG
    GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC
    GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC
    CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT
    GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT
    GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA
    TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC
    GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG
    CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA
    GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC
    TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG
    AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC
    CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC
    GCCGATGACA ACCTTGGGCC ACAGCTGGCC GAGCTGACCA ATAGGAGGCG TGGACGTCTG
    CGCTGGTTCA GTCATTCTAC TCGCTCCACA CACTCCACCA GCAGCCATGC CAGCCTCCCA
    GCCAGTGACA CCGGTTCCAT GACAGAGACC CAAGGCGATG AGGATGAGGA GGAGGGGGCT
    CTGGCCAGCT GTACACGCTG CCTAAACAAG ATCATGAAAA CCAGAGTCTG CCGCCGCCTC
    CGCCGAGCCA ACCGGGTCCT TCGGGCACGC TGCCGTCGGG CAGTGAAGTC CAATGCCTGC
    TACTGGGCTG TGCTGTTGCT CGTCTTCCTC AACACGTTGA CCATCGCCTC TGAGCACCAC
    GGGCAGCCTG TGTGGCTCAC CCAGATCCAG GAGTATGCCA ACAAAGTGTT GCTCTGTCTG
    TTCACGGTGG AGATGCTTCT CAAATTGTAC GGTCTGGGCC CCTCTGCCTA TGTGTCTTCC
    TTCTTCAACC GCTTTGACTG CTTTGTGGTC TGTGGGGGCA TCCTAGAGAC CACCTTGGTG
    GAGGTGGGTG CCATGCAGCC CTTGGGCATC TCAGTGCTCC GATGTGTGCG CCTCCTCAGG
    ATCTTTAAGG TCACCAGACA CTGGGCTTCT CTGAGCAATC TGGTGGCATC CCTGCTCAAT
    TCAATGAAAT CCATCGCATC CTTGCTGCTT CTCCTCTTCC TCTTCATCAT TATCTTCTCC
    CTGCTTGGCA TGCAGCTGTT TGGGGGCAAG TTCAACTTTG ACCAGACCCA CACCAAGCGA
    AGCACCTTTG ACACGTTCCC CCAGGCCCTC CTCACTGTCT TTCAGATCCT GACAGGTGAG
    GACTGGAACG TGGTCATGTA TGATGGTATC ATGGCATATG GTGGCCCCTT CTTCCCAGGA
    ATGTTGGTGT GCATCTATTT CATCATTCTC TTCATCTGTG GCAACTACAT CCTGTTGAAC
    GTGTTTCTTG CCATTGCTGT GGACAACCTG GCCAGTGGAG ATGCAGGCAC TGCCAAGGAC
    AAGGGCGGGG AGAAGAGCAA TGAGAAGGAT CTCCCACAGG AGAATGAAGG CCTGGTGCCT
    GGTGTGGAGA AAGAGGAAGA GGAGGGTGCA AGGAGGGAAG GAGCAGACAT GGAGGAGGAG
    GAGGAGGAGG AAGAAGAGGA AGAAGAGGAA GAAGAGGAAG AGGGTGCAGG GGGTGTGGAA
    CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAGGG CAGCGCCTTC
    TTCTGCCTCA GCCAAACCAA CCCGCTGAGG AAGGGCTGCC ACACCCTCAT CCACCATCAT
    GTCTTCACCA ATCTTATCCT GGTGTTCATC ATCCTCAGCA GTGTGTCCCT GGCCGCTGAG
    GACCCCATCC GAGCCCACTC CTTCCGCAAC CATATTCTGG GTTACTTCGA TTATGCCTTC
    ACCTCCATTT TCACTGTGGA GATTCTACTA AAGATGACAG TGTTTGGGGC CTTCCTGCAC
    CGCGGCTCCT TCTGCCGTAG CTGGTTTAAT ATGTTGGATC TGCTGGTGGT CAGTGTGTCC
    CTCATCTCCT TTGGCATCCA CTCCAGCGCC ATCTCGGTGG TGAAGATTCT GCGAGTACTC
    CGAGTACTGC GGCCCCTCCG AGCCATCAAC AGGGCCAAGG GACTCAAGCA TGTGGTGCAG
    TGTGTATTTG TGGCCATCCG GACCATCGGA AACATCATGA TTGTCACCAC ACTTCTGCAA
    TTTATGTTCG CCTGCATCGG GGTGCAGCTC TTCAAGGGGA AATTCTACAC CTGCACGGAC
    GAGGCCAAAC ACACCCCTCA AGAATGCAAG GGCTCCTTCC TGGTATACCC AGATGGAGAC
    GTGTCACGGC CCCTGGTCCG GGAGCGGCTC TGGGTCAACA GTGATTTCAA CTTTGACAAT
    GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCCACCT TTGAAGGCTG GCCTGCACTG
    CTATACAAGG CCATCGATGC ATATGCAGAG GACCACGGCC CCATCTATAA TTACCGTGTG
    GAGATCTCAG TGTTCTTCAT TGTCTACATC ATCATCATTG CGTTCTTCAT GATGAACATC
    TTCGTGGGCT TCGTCATCAT CACTTTCCGT GCCCAGGGCG AGCAGGAGTA CCAAAACTGT
    GAGCTGGACA AGAACCAGCG TCAATGTGTG GAATATGCCC TCAAGGCCCA GCCACTCCGC
    CGTTACATCC CCAAGAACCC GCATCAGTAT CGTGTGTGGG CCACTGTGAA CTCTGCTGCC
    TTTGAGTACC TGATGTTCCT GCTCATCCTG CTCAACACAG TTGCCCTAGC CATGCAGCAC
    TATGAGCAGA CTGCTCCCTT CAACTATGCC ATGGACATCC TCAACATGGT CTTCACTGGC
    CTCTTCACTA TTGAGATGGT GCTCAAAATC ATCGCCTTCA AGCCCAAGCA TTACTTCACT
    GATGCCTGGA ACACGTTTGA CGCTCTTATT GTGGTGGGCA GCATAGTGGA TATTGCCGTC
    ACTGAAGTCA ATAATGGTGG CCACCTTGGC GAGAGCTCTG AGGACAGCTC CCGCATTTCC
    ATTACCTTCT TTCGCCTCTT CCGAGTTATG CGGCTGGTCA AGCTTCTCAG TAAGGGTGAA
    GGGATCCGCA CATTGCTCTG GACATTCATC AAGTCCTTCC AGGCCTTGCC CTATGTGGCT
    CTTCTCATCG CAATGATATT CTTCATCTAT GCCGTCATTG GCATGCAGAT GTTCGGCAAG
    GTGGCTCTTC AGGATGGCAC ACAGATAAAC CGAAACAACA ACTTCCAGAC CTTTCCACAG
    GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAGGAGAT AATGCTTGCC
    AGCCTTCCCG GAAATCGGTG TGATCCTGAG TCTGACTTCG GCCCTGGTGA AGAGTTTACC
    TGTGGTAGCA ATTTTGCCAT CGCCTATTTC ATCAGCTTCT TCATGCTCTG TGCCTTCCTG
    ATCATAAATC TCTTTGTGGC TGTGATCATG GACAACTTTG ATTATCTCAC CAGAGATTGG
    TCCATCCTGG GCCCCCATCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCT
    GGGGCCAAGG GCCGCATCAA ACACTTGGAT GTGGTTGCCC TGCTGAGACG TATCCAGCCC
    CCTCTGGGAT TTGGGAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT TGTGGCAATG
    AACATGCCCC TCAACTCAGA TGGGACGGTG ACATTCAACG CCACACTCTT TGCCCTGGTC
    CGGACATCCC TGAAGATCAA AACAGAAGGG AACCTGGAGC AAGCCAACCA GGAGCTGCGG
    ATTGTCATCA AAAAGATCTG GAAGCGGATG AAACAGAAGC TGCTAGATGA GGTCATCCCC
    CCACCAGACG AGGAGGAGGT CACCGTGGGC AAATTCTACG CCACATTTCT GATCCAGGAC
    TATTTCCGCA AATTCCGGCG GAGGAAAGAA AAAGGGCTAC TAGGCAACGA CGCCGCCCCT
    AGCACCTCTT CCGCCCTTCA GGCTGGTCTG CGGAGCCTGC AGGACTTGGG TCCTGAGATG
    CGGCAGGCCC TCACCTGTGA CACAGAGGAG GAGGAAGAAG AGGGGCAGGA GGGAGTGGAG
    GAGGAAGATG AAAAGGACTT GGAAACTAAC AAAGCCACGA TGGTCTCCCA GCCCTCAGCT
    CGCCGGGGCT CCGGGATTTC TGTGTCTCTG CCTGTCGGGG ACAGACTTCC AGATTCACTC
    TCCTTTGGGC CCAGTGATGA TGACAGGGGG ACTCCCACCT CCAGTCAGCC CAGTGTGCCC
    CAGGCTGGAT CCAACACCCA CAGGAGAGGC TCTGGGGCTC TCATTTTCAC CATCCCAGAA
    GAAGGAAATT CTCAGCCCAA GGGAACCAAA GGGCAAAACA AGCAAGATGA GGATGAGGAA
    GTCCCTGATC GGCTTTCCTA CCTAGATGAG CAGGCAGGGA CTCCCCCGTG CTCAGTCCTT
    TTGCCACCTC ACAGAGCTCA GAGATACATG GATGGGCACC TGGTACCACG CCGCCGTCTG
    CTGCCCCCCA CACCTGCAGG TCGGAAGCCC TCCTTCACCA TCCAGTGTCT GCAGCGCCAG
    GGCAGTTGTG AGGATTTACC CATCCCAGGC ACCTATCATC GTGGGCGAAA TTCAGGGCCC
    AATAGGGCTC AGGGTTCCTG GGCAACACCA CCTCAGCGGG GTCGGCTCCT GTATGCCCCG
    CTGTTGTTGG TGGAAGAGGG CGCAGCGGGG GAGGGGTACC TCGGCAGATC CAGTGGCCCA
    CTGCGCACCT TCACCTGTCT GCACGTGCCT GGAACCCACT CGGACCCCAG CCATGGGAAG
    AGGGGCAGTG CCGACAGCTT GGTGGAGGCT GTGCTTATCT CAGAGGGTCT GGGCCTCTTT
    GCTCGAGACC CACGTTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC GTGTCGCCTG
    ACGCTGGATG AGATGGACAA TGCTGCCAGT GACCTGCTGG CACAGGGAAC CAGCTCTCTC
    TATAGCGACG AGGAGTCCAT CCTCTCCCGC TTCGATGAGG AGGACTTGGG AGACGAGATG
    GCCTGCGTCC ACGCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21801
    Clone ID Related Accession (Same CDS sequence) NM_001256789.3 , NM_001256789.2
    Accession Version NM_001256789.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5901bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ224874.1, AC232271.2 and AF201304.1. On Aug 21, 2013 this sequence version replaced NM_001256789.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ224874.1, JF701915.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
    GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT
    GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG
    GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT
    CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG
    ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG
    GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC
    GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC
    CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT
    GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT
    GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA
    TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC
    GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG
    CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA
    GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC
    TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG
    AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC
    CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC
    GCCGATGACA ACCTTGGGCC ACAGCTGGCC GAGCTGACCA ATAGGAGGCG TGGACGTCTG
    CGCTGGTTCA GTCATTCTAC TCGCTCCACA CACTCCACCA GCAGCCATGC CAGCCTCCCA
    GCCAGTGACA CCGGTTCCAT GACAGAGACC CAAGGCGATG AGGATGAGGA GGAGGGGGCT
    CTGGCCAGCT GTACACGCTG CCTAAACAAG ATCATGAAAA CCAGAGTCTG CCGCCGCCTC
    CGCCGAGCCA ACCGGGTCCT TCGGGCACGC TGCCGTCGGG CAGTGAAGTC CAATGCCTGC
    TACTGGGCTG TGCTGTTGCT CGTCTTCCTC AACACGTTGA CCATCGCCTC TGAGCACCAC
    GGGCAGCCTG TGTGGCTCAC CCAGATCCAG GAGTATGCCA ACAAAGTGTT GCTCTGTCTG
    TTCACGGTGG AGATGCTTCT CAAATTGTAC GGTCTGGGCC CCTCTGCCTA TGTGTCTTCC
    TTCTTCAACC GCTTTGACTG CTTTGTGGTC TGTGGGGGCA TCCTAGAGAC CACCTTGGTG
    GAGGTGGGTG CCATGCAGCC CTTGGGCATC TCAGTGCTCC GATGTGTGCG CCTCCTCAGG
    ATCTTTAAGG TCACCAGACA CTGGGCTTCT CTGAGCAATC TGGTGGCATC CCTGCTCAAT
    TCAATGAAAT CCATCGCATC CTTGCTGCTT CTCCTCTTCC TCTTCATCAT TATCTTCTCC
    CTGCTTGGCA TGCAGCTGTT TGGGGGCAAG TTCAACTTTG ACCAGACCCA CACCAAGCGA
    AGCACCTTTG ACACGTTCCC CCAGGCCCTC CTCACTGTCT TTCAGATCCT GACAGGTGAG
    GACTGGAACG TGGTCATGTA TGATGGTATC ATGGCATATG GTGGCCCCTT CTTCCCAGGA
    ATGTTGGTGT GCATCTATTT CATCATTCTC TTCATCTGTG GCAACTACAT CCTGTTGAAC
    GTGTTTCTTG CCATTGCTGT GGACAACCTG GCCAGTGGAG ATGCAGGCAC TGCCAAGGAC
    AAGGGCGGGG AGAAGAGCAA TGAGAAGGAT CTCCCACAGG AGAATGAAGG CCTGGTGCCT
    GGTGTGGAGA AAGAGGAAGA GGAGGGTGCA AGGAGGGAAG GAGCAGACAT GGAGGAGGAG
    GAGGAGGAGG AAGAAGAGGA AGAAGAGGAA GAAGAGGAAG AGGGTGCAGG GGGTGTGGAA
    CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAGGG CAGCGCCTTC
    TTCTGCCTCA GCCAAACCAA CCCGCTGAGG AAGGGCTGCC ACACCCTCAT CCACCATCAT
    GTCTTCACCA ATCTTATCCT GGTGTTCATC ATCCTCAGCA GTGTGTCCCT GGCCGCTGAG
    GACCCCATCC GAGCCCACTC CTTCCGCAAC CATATTCTGG GTTACTTCGA TTATGCCTTC
    ACCTCCATTT TCACTGTGGA GATTCTACTA AAGATGACAG TGTTTGGGGC CTTCCTGCAC
    CGCGGCTCCT TCTGCCGTAG CTGGTTTAAT ATGTTGGATC TGCTGGTGGT CAGTGTGTCC
    CTCATCTCCT TTGGCATCCA CTCCAGCGCC ATCTCGGTGG TGAAGATTCT GCGAGTACTC
    CGAGTACTGC GGCCCCTCCG AGCCATCAAC AGGGCCAAGG GACTCAAGCA TGTGGTGCAG
    TGTGTATTTG TGGCCATCCG GACCATCGGA AACATCATGA TTGTCACCAC ACTTCTGCAA
    TTTATGTTCG CCTGCATCGG GGTGCAGCTC TTCAAGGGGA AATTCTACAC CTGCACGGAC
    GAGGCCAAAC ACACCCCTCA AGAATGCAAG GGCTCCTTCC TGGTATACCC AGATGGAGAC
    GTGTCACGGC CCCTGGTCCG GGAGCGGCTC TGGGTCAACA GTGATTTCAA CTTTGACAAT
    GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCCACCT TTGAAGGCTG GCCTGCACTG
    CTATACAAGG CCATCGATGC ATATGCAGAG GACCACGGCC CCATCTATAA TTACCGTGTG
    GAGATCTCAG TGTTCTTCAT TGTCTACATC ATCATCATTG CGTTCTTCAT GATGAACATC
    TTCGTGGGCT TCGTCATCAT CACTTTCCGT GCCCAGGGCG AGCAGGAGTA CCAAAACTGT
    GAGCTGGACA AGAACCAGCG TCAATGTGTG GAATATGCCC TCAAGGCCCA GCCACTCCGC
    CGTTACATCC CCAAGAACCC GCATCAGTAT CGTGTGTGGG CCACTGTGAA CTCTGCTGCC
    TTTGAGTACC TGATGTTCCT GCTCATCCTG CTCAACACAG TTGCCCTAGC CATGCAGCAC
    TATGAGCAGA CTGCTCCCTT CAACTATGCC ATGGACATCC TCAACATGGT CTTCACTGGC
    CTCTTCACTA TTGAGATGGT GCTCAAAATC ATCGCCTTCA AGCCCAAGCA TTACTTCACT
    GATGCCTGGA ACACGTTTGA CGCTCTTATT GTGGTGGGCA GCATAGTGGA TATTGCCGTC
    ACTGAAGTCA ATAATGGTGG CCACCTTGGC GAGAGCTCTG AGGACAGCTC CCGCATTTCC
    ATTACCTTCT TTCGCCTCTT CCGAGTTATG CGGCTGGTCA AGCTTCTCAG TAAGGGTGAA
    GGGATCCGCA CATTGCTCTG GACATTCATC AAGTCCTTCC AGGCCTTGCC CTATGTGGCT
    CTTCTCATCG CAATGATATT CTTCATCTAT GCCGTCATTG GCATGCAGAT GTTCGGCAAG
    GTGGCTCTTC AGGATGGCAC ACAGATAAAC CGAAACAACA ACTTCCAGAC CTTTCCACAG
    GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAGGAGAT AATGCTTGCC
    AGCCTTCCCG GAAATCGGTG TGATCCTGAG TCTGACTTCG GCCCTGGTGA AGAGTTTACC
    TGTGGTAGCA ATTTTGCCAT CGCCTATTTC ATCAGCTTCT TCATGCTCTG TGCCTTCCTG
    ATCATAAATC TCTTTGTGGC TGTGATCATG GACAACTTTG ATTATCTCAC CAGAGATTGG
    TCCATCCTGG GCCCCCATCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCT
    GGGGCCAAGG GCCGCATCAA ACACTTGGAT GTGGTTGCCC TGCTGAGACG TATCCAGCCC
    CCTCTGGGAT TTGGGAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT TGTGGCAATG
    AACATGCCCC TCAACTCAGA TGGGACGGTG ACATTCAACG CCACACTCTT TGCCCTGGTC
    CGGACATCCC TGAAGATCAA AACAGAAGGG AACCTGGAGC AAGCCAACCA GGAGCTGCGG
    ATTGTCATCA AAAAGATCTG GAAGCGGATG AAACAGAAGC TGCTAGATGA GGTCATCCCC
    CCACCAGACG AGGAGGAGGT CACCGTGGGC AAATTCTACG CCACATTTCT GATCCAGGAC
    TATTTCCGCA AATTCCGGCG GAGGAAAGAA AAAGGGCTAC TAGGCAACGA CGCCGCCCCT
    AGCACCTCTT CCGCCCTTCA GGCTGGTCTG CGGAGCCTGC AGGACTTGGG TCCTGAGATG
    CGGCAGGCCC TCACCTGTGA CACAGAGGAG GAGGAAGAAG AGGGGCAGGA GGGAGTGGAG
    GAGGAAGATG AAAAGGACTT GGAAACTAAC AAAGCCACGA TGGTCTCCCA GCCCTCAGCT
    CGCCGGGGCT CCGGGATTTC TGTGTCTCTG CCTGTCGGGG ACAGACTTCC AGATTCACTC
    TCCTTTGGGC CCAGTGATGA TGACAGGGGG ACTCCCACCT CCAGTCAGCC CAGTGTGCCC
    CAGGCTGGAT CCAACACCCA CAGGAGAGGC TCTGGGGCTC TCATTTTCAC CATCCCAGAA
    GAAGGAAATT CTCAGCCCAA GGGAACCAAA GGGCAAAACA AGCAAGATGA GGATGAGGAA
    GTCCCTGATC GGCTTTCCTA CCTAGATGAG CAGGCAGGGA CTCCCCCGTG CTCAGTCCTT
    TTGCCACCTC ACAGAGCTCA GAGATACATG GATGGGCACC TGGTACCACG CCGCCGTCTG
    CTGCCCCCCA CACCTGCAGG TCGGAAGCCC TCCTTCACCA TCCAGTGTCT GCAGCGCCAG
    GGCAGTTGTG AGGATTTACC CATCCCAGGC ACCTATCATC GTGGGCGAAA TTCAGGGCCC
    AATAGGGCTC AGGGTTCCTG GGCAACACCA CCTCAGCGGG GTCGGCTCCT GTATGCCCCG
    CTGTTGTTGG TGGAAGAGGG CGCAGCGGGG GAGGGGTACC TCGGCAGATC CAGTGGCCCA
    CTGCGCACCT TCACCTGTCT GCACGTGCCT GGAACCCACT CGGACCCCAG CCATGGGAAG
    AGGGGCAGTG CCGACAGCTT GGTGGAGGCT GTGCTTATCT CAGAGGGTCT GGGCCTCTTT
    GCTCGAGACC CACGTTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC GTGTCGCCTG
    ACGCTGGATG AGATGGACAA TGCTGCCAGT GACCTGCTGG CACAGGGAAC CAGCTCTCTC
    TATAGCGACG AGGAGTCCAT CCTCTCCCGC TTCGATGAGG AGGACTTGGG AGACGAGATG
    GCCTGCGTCC ACGCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243718.1
    CDS63..5963
    Translation

    Target ORF information:

    RefSeq Version NM_001256789.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256789.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
    GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT
    GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG
    GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT
    CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG
    ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG
    GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC
    GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC
    CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT
    GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT
    GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA
    TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC
    GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG
    CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA
    GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC
    TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG
    AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC
    CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC
    GCCGATGACA ACCTTGGGCC ACAGCTGGCC GAGCTGACCA ATAGGAGGCG TGGACGTCTG
    CGCTGGTTCA GTCATTCTAC TCGCTCCACA CACTCCACCA GCAGCCATGC CAGCCTCCCA
    GCCAGTGACA CCGGTTCCAT GACAGAGACC CAAGGCGATG AGGATGAGGA GGAGGGGGCT
    CTGGCCAGCT GTACACGCTG CCTAAACAAG ATCATGAAAA CCAGAGTCTG CCGCCGCCTC
    CGCCGAGCCA ACCGGGTCCT TCGGGCACGC TGCCGTCGGG CAGTGAAGTC CAATGCCTGC
    TACTGGGCTG TGCTGTTGCT CGTCTTCCTC AACACGTTGA CCATCGCCTC TGAGCACCAC
    GGGCAGCCTG TGTGGCTCAC CCAGATCCAG GAGTATGCCA ACAAAGTGTT GCTCTGTCTG
    TTCACGGTGG AGATGCTTCT CAAATTGTAC GGTCTGGGCC CCTCTGCCTA TGTGTCTTCC
    TTCTTCAACC GCTTTGACTG CTTTGTGGTC TGTGGGGGCA TCCTAGAGAC CACCTTGGTG
    GAGGTGGGTG CCATGCAGCC CTTGGGCATC TCAGTGCTCC GATGTGTGCG CCTCCTCAGG
    ATCTTTAAGG TCACCAGACA CTGGGCTTCT CTGAGCAATC TGGTGGCATC CCTGCTCAAT
    TCAATGAAAT CCATCGCATC CTTGCTGCTT CTCCTCTTCC TCTTCATCAT TATCTTCTCC
    CTGCTTGGCA TGCAGCTGTT TGGGGGCAAG TTCAACTTTG ACCAGACCCA CACCAAGCGA
    AGCACCTTTG ACACGTTCCC CCAGGCCCTC CTCACTGTCT TTCAGATCCT GACAGGTGAG
    GACTGGAACG TGGTCATGTA TGATGGTATC ATGGCATATG GTGGCCCCTT CTTCCCAGGA
    ATGTTGGTGT GCATCTATTT CATCATTCTC TTCATCTGTG GCAACTACAT CCTGTTGAAC
    GTGTTTCTTG CCATTGCTGT GGACAACCTG GCCAGTGGAG ATGCAGGCAC TGCCAAGGAC
    AAGGGCGGGG AGAAGAGCAA TGAGAAGGAT CTCCCACAGG AGAATGAAGG CCTGGTGCCT
    GGTGTGGAGA AAGAGGAAGA GGAGGGTGCA AGGAGGGAAG GAGCAGACAT GGAGGAGGAG
    GAGGAGGAGG AAGAAGAGGA AGAAGAGGAA GAAGAGGAAG AGGGTGCAGG GGGTGTGGAA
    CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAGGG CAGCGCCTTC
    TTCTGCCTCA GCCAAACCAA CCCGCTGAGG AAGGGCTGCC ACACCCTCAT CCACCATCAT
    GTCTTCACCA ATCTTATCCT GGTGTTCATC ATCCTCAGCA GTGTGTCCCT GGCCGCTGAG
    GACCCCATCC GAGCCCACTC CTTCCGCAAC CATATTCTGG GTTACTTCGA TTATGCCTTC
    ACCTCCATTT TCACTGTGGA GATTCTACTA AAGATGACAG TGTTTGGGGC CTTCCTGCAC
    CGCGGCTCCT TCTGCCGTAG CTGGTTTAAT ATGTTGGATC TGCTGGTGGT CAGTGTGTCC
    CTCATCTCCT TTGGCATCCA CTCCAGCGCC ATCTCGGTGG TGAAGATTCT GCGAGTACTC
    CGAGTACTGC GGCCCCTCCG AGCCATCAAC AGGGCCAAGG GACTCAAGCA TGTGGTGCAG
    TGTGTATTTG TGGCCATCCG GACCATCGGA AACATCATGA TTGTCACCAC ACTTCTGCAA
    TTTATGTTCG CCTGCATCGG GGTGCAGCTC TTCAAGGGGA AATTCTACAC CTGCACGGAC
    GAGGCCAAAC ACACCCCTCA AGAATGCAAG GGCTCCTTCC TGGTATACCC AGATGGAGAC
    GTGTCACGGC CCCTGGTCCG GGAGCGGCTC TGGGTCAACA GTGATTTCAA CTTTGACAAT
    GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCCACCT TTGAAGGCTG GCCTGCACTG
    CTATACAAGG CCATCGATGC ATATGCAGAG GACCACGGCC CCATCTATAA TTACCGTGTG
    GAGATCTCAG TGTTCTTCAT TGTCTACATC ATCATCATTG CGTTCTTCAT GATGAACATC
    TTCGTGGGCT TCGTCATCAT CACTTTCCGT GCCCAGGGCG AGCAGGAGTA CCAAAACTGT
    GAGCTGGACA AGAACCAGCG TCAATGTGTG GAATATGCCC TCAAGGCCCA GCCACTCCGC
    CGTTACATCC CCAAGAACCC GCATCAGTAT CGTGTGTGGG CCACTGTGAA CTCTGCTGCC
    TTTGAGTACC TGATGTTCCT GCTCATCCTG CTCAACACAG TTGCCCTAGC CATGCAGCAC
    TATGAGCAGA CTGCTCCCTT CAACTATGCC ATGGACATCC TCAACATGGT CTTCACTGGC
    CTCTTCACTA TTGAGATGGT GCTCAAAATC ATCGCCTTCA AGCCCAAGCA TTACTTCACT
    GATGCCTGGA ACACGTTTGA CGCTCTTATT GTGGTGGGCA GCATAGTGGA TATTGCCGTC
    ACTGAAGTCA ATAATGGTGG CCACCTTGGC GAGAGCTCTG AGGACAGCTC CCGCATTTCC
    ATTACCTTCT TTCGCCTCTT CCGAGTTATG CGGCTGGTCA AGCTTCTCAG TAAGGGTGAA
    GGGATCCGCA CATTGCTCTG GACATTCATC AAGTCCTTCC AGGCCTTGCC CTATGTGGCT
    CTTCTCATCG CAATGATATT CTTCATCTAT GCCGTCATTG GCATGCAGAT GTTCGGCAAG
    GTGGCTCTTC AGGATGGCAC ACAGATAAAC CGAAACAACA ACTTCCAGAC CTTTCCACAG
    GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAGGAGAT AATGCTTGCC
    AGCCTTCCCG GAAATCGGTG TGATCCTGAG TCTGACTTCG GCCCTGGTGA AGAGTTTACC
    TGTGGTAGCA ATTTTGCCAT CGCCTATTTC ATCAGCTTCT TCATGCTCTG TGCCTTCCTG
    ATCATAAATC TCTTTGTGGC TGTGATCATG GACAACTTTG ATTATCTCAC CAGAGATTGG
    TCCATCCTGG GCCCCCATCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCT
    GGGGCCAAGG GCCGCATCAA ACACTTGGAT GTGGTTGCCC TGCTGAGACG TATCCAGCCC
    CCTCTGGGAT TTGGGAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT TGTGGCAATG
    AACATGCCCC TCAACTCAGA TGGGACGGTG ACATTCAACG CCACACTCTT TGCCCTGGTC
    CGGACATCCC TGAAGATCAA AACAGAAGGG AACCTGGAGC AAGCCAACCA GGAGCTGCGG
    ATTGTCATCA AAAAGATCTG GAAGCGGATG AAACAGAAGC TGCTAGATGA GGTCATCCCC
    CCACCAGACG AGGAGGAGGT CACCGTGGGC AAATTCTACG CCACATTTCT GATCCAGGAC
    TATTTCCGCA AATTCCGGCG GAGGAAAGAA AAAGGGCTAC TAGGCAACGA CGCCGCCCCT
    AGCACCTCTT CCGCCCTTCA GGCTGGTCTG CGGAGCCTGC AGGACTTGGG TCCTGAGATG
    CGGCAGGCCC TCACCTGTGA CACAGAGGAG GAGGAAGAAG AGGGGCAGGA GGGAGTGGAG
    GAGGAAGATG AAAAGGACTT GGAAACTAAC AAAGCCACGA TGGTCTCCCA GCCCTCAGCT
    CGCCGGGGCT CCGGGATTTC TGTGTCTCTG CCTGTCGGGG ACAGACTTCC AGATTCACTC
    TCCTTTGGGC CCAGTGATGA TGACAGGGGG ACTCCCACCT CCAGTCAGCC CAGTGTGCCC
    CAGGCTGGAT CCAACACCCA CAGGAGAGGC TCTGGGGCTC TCATTTTCAC CATCCCAGAA
    GAAGGAAATT CTCAGCCCAA GGGAACCAAA GGGCAAAACA AGCAAGATGA GGATGAGGAA
    GTCCCTGATC GGCTTTCCTA CCTAGATGAG CAGGCAGGGA CTCCCCCGTG CTCAGTCCTT
    TTGCCACCTC ACAGAGCTCA GAGATACATG GATGGGCACC TGGTACCACG CCGCCGTCTG
    CTGCCCCCCA CACCTGCAGG TCGGAAGCCC TCCTTCACCA TCCAGTGTCT GCAGCGCCAG
    GGCAGTTGTG AGGATTTACC CATCCCAGGC ACCTATCATC GTGGGCGAAA TTCAGGGCCC
    AATAGGGCTC AGGGTTCCTG GGCAACACCA CCTCAGCGGG GTCGGCTCCT GTATGCCCCG
    CTGTTGTTGG TGGAAGAGGG CGCAGCGGGG GAGGGGTACC TCGGCAGATC CAGTGGCCCA
    CTGCGCACCT TCACCTGTCT GCACGTGCCT GGAACCCACT CGGACCCCAG CCATGGGAAG
    AGGGGCAGTG CCGACAGCTT GGTGGAGGCT GTGCTTATCT CAGAGGGTCT GGGCCTCTTT
    GCTCGAGACC CACGTTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC GTGTCGCCTG
    ACGCTGGATG AGATGGACAA TGCTGCCAGT GACCTGCTGG CACAGGGAAC CAGCTCTCTC
    TATAGCGACG AGGAGTCCAT CCTCTCCCGC TTCGATGAGG AGGACTTGGG AGACGAGATG
    GCCTGCGTCC ACGCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56583
    Clone ID Related Accession (Same CDS sequence) XM_011543983.2
    Accession Version XM_011543983.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
    GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC
    TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC
    AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG
    ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA
    CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA
    CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG
    AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA
    GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC
    TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG
    ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT
    TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG
    CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG
    TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT
    GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC
    CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC
    GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG
    GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT
    GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT
    AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC
    AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG
    GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC
    AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA
    GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC
    ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC
    AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC
    TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC
    CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA
    TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG
    GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC
    TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC
    CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT
    CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT
    GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC
    AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT
    GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG
    AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA
    GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG
    GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC
    CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC
    ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT
    GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT
    TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG
    TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG
    AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA
    CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT
    GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA
    TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG
    GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT
    GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT
    GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC
    ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG
    TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG
    CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC
    AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC
    ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT
    GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC
    AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG
    CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC
    ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AGCTCTGAGG ACAGCTCCCG CATTTCCATT
    ACCTTCTTTC GCCTCTTCCG AGTTATGCGG CTGGTCAAGC TTCTCAGTAA GGGTGAAGGG
    ATCCGCACAT TGCTCTGGAC ATTCATCAAG TCCTTCCAGG CCTTGCCCTA TGTGGCTCTT
    CTCATCGCAA TGATATTCTT CATCTATGCC GTCATTGGCA TGCAGATGTT CGGCAAGGTG
    GCTCTTCAGG ATGGCACACA GATAAACCGA AACAACAACT TCCAGACCTT TCCACAGGCT
    GTGCTGCTTC TGTTCAGGTG TGCCACTGGT GAGGCATGGC AGGAGATAAT GCTTGCCAGC
    CTTCCCGGAA ATCGGTGTGA TCCTGAGTCT GACTTCGGCC CTGGTGAAGA GTTTACCTGT
    GGTAGCAATT TTGCCATCGC CTATTTCATC AGCTTCTTCA TGCTCTGTGC CTTCCTGATC
    ATAAATCTCT TTGTGGCTGT GATCATGGAC AACTTTGATT ATCTCACCAG AGATTGGTCC
    ATCCTGGGCC CCCATCACCT TGATGAATTC AAGAGGATCT GGTCTGAATA TGACCCTGGG
    GCCAAGGGCC GCATCAAACA CTTGGATGTG GTTGCCCTGC TGAGACGTAT CCAGCCCCCT
    CTGGGATTTG GGAAGCTGTG CCCACACCGA GTGGCCTGCA AGAGACTTGT GGCAATGAAC
    ATGCCCCTCA ACTCAGATGG GACGGTGACA TTCAACGCCA CACTCTTTGC CCTGGTCCGG
    ACATCCCTGA AGATCAAAAC AGAAGGGAAC CTGGAGCAAG CCAACCAGGA GCTGCGGATT
    GTCATCAAAA AGATCTGGAA GCGGATGAAA CAGAAGCTGC TAGATGAGGT CATCCCCCCA
    CCAGACGAGG AGGAGGTCAC CGTGGGCAAA TTCTACGCCA CATTTCTGAT CCAGGACTAT
    TTCCGCAAAT TCCGGCGGAG GAAAGAAAAA GGGCTACTAG GCAACGACGC CGCCCCTAGC
    ACCTCTTCCG CCCTTCAGGC TGGTCTGCGG AGCCTGCAGG ACTTGGGTCC TGAGATGCGG
    CAGGCCCTCA CCTGTGACAC AGAGGAGGAG GAAGAAGAGG GGCAGGAGGG AGTGGAGGAG
    GAAGATGAAA AGGACTTGGA AACTAACAAA GCCACGATGG TCTCCCAGCC CTCAGCTCGC
    CGGGGCTCCG GGATTTCTGT GTCTCTGCCT GTCGGGGACA GACTTCCAGA TTCACTCTCC
    TTTGGGCCCA GTGATGATGA CAGGGGGACT CCCACCTCCA GTCAGCCCAG TGTGCCCCAG
    GCTGGATCCA ACACCCACAG GAGAGGCTCT GGGGCTCTCA TTTTCACCAT CCCAGAAGAA
    GGAAATTCTC AGCCCAAGGG AACCAAAGGG CAAAACAAGC AAGATGAGGA TGAGGAAGTC
    CCTGATCGGC TTTCCTACCT AGATGAGCAG GCAGGGACTC CCCCGTGCTC AGTCCTTTTG
    CCACCTCACA GAGCTCAGAG ATACATGGAT GGGCACCTGG TACCACGCCG CCGTCTGCTG
    CCCCCCACAC CTGCAGGTCG GAAGCCCTCC TTCACCATCC AGTGTCTGCA GCGCCAGGGC
    AGTTGTGAGG ATTTACCCAT CCCAGGCACC TATCATCGTG GGCGAAATTC AGGGCCCAAT
    AGGGCTCAGG GTTCCTGGGC AACACCACCT CAGCGGGGTC GGCTCCTGTA TGCCCCGCTG
    TTGTTGGTGG AAGAGGGCGC AGCGGGGGAG GGGTACCTCG GCAGATCCAG TGGCCCACTG
    CGCACCTTCA CCTGTCTGCA CGTGCCTGGA ACCCACTCGG ACCCCAGCCA TGGGAAGAGG
    GGCAGTGCCG ACAGCTTGGT GGAGGCTGTG CTTATCTCAG AGGGTCTGGG CCTCTTTGCT
    CGAGACCCAC GTTTCGTGGC CCTGGCCAAG CAGGAGATTG CAGATGCGTG TCGCCTGACG
    CTGGATGAGA TGGACAATGC TGCCAGTGAC CTGCTGGCAC AGGGAACCAG CTCTCTCTAT
    AGCGACGAGG AGTCCATCCT CTCCCGCTTC GATGAGGAGG ACTTGGGAGA CGAGATGGCC
    TGCGTCCACG CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011542285.1
    CDS96..5813
    Translation

    Target ORF information:

    RefSeq Version XM_011543983.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011543983.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
    GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC
    TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC
    AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG
    ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA
    CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA
    CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG
    AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA
    GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC
    TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG
    ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT
    TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG
    CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG
    TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT
    GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC
    CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC
    GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG
    GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT
    GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT
    AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC
    AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG
    GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC
    AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA
    GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC
    ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC
    AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC
    TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC
    CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA
    TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG
    GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC
    TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC
    CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT
    CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT
    GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC
    AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT
    GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG
    AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA
    GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG
    GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC
    CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC
    ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT
    GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT
    TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG
    TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG
    AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA
    CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT
    GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA
    TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG
    GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT
    GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT
    GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC
    ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG
    TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG
    CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC
    AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC
    ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT
    GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC
    AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG
    CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC
    ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AGCTCTGAGG ACAGCTCCCG CATTTCCATT
    ACCTTCTTTC GCCTCTTCCG AGTTATGCGG CTGGTCAAGC TTCTCAGTAA GGGTGAAGGG
    ATCCGCACAT TGCTCTGGAC ATTCATCAAG TCCTTCCAGG CCTTGCCCTA TGTGGCTCTT
    CTCATCGCAA TGATATTCTT CATCTATGCC GTCATTGGCA TGCAGATGTT CGGCAAGGTG
    GCTCTTCAGG ATGGCACACA GATAAACCGA AACAACAACT TCCAGACCTT TCCACAGGCT
    GTGCTGCTTC TGTTCAGGTG TGCCACTGGT GAGGCATGGC AGGAGATAAT GCTTGCCAGC
    CTTCCCGGAA ATCGGTGTGA TCCTGAGTCT GACTTCGGCC CTGGTGAAGA GTTTACCTGT
    GGTAGCAATT TTGCCATCGC CTATTTCATC AGCTTCTTCA TGCTCTGTGC CTTCCTGATC
    ATAAATCTCT TTGTGGCTGT GATCATGGAC AACTTTGATT ATCTCACCAG AGATTGGTCC
    ATCCTGGGCC CCCATCACCT TGATGAATTC AAGAGGATCT GGTCTGAATA TGACCCTGGG
    GCCAAGGGCC GCATCAAACA CTTGGATGTG GTTGCCCTGC TGAGACGTAT CCAGCCCCCT
    CTGGGATTTG GGAAGCTGTG CCCACACCGA GTGGCCTGCA AGAGACTTGT GGCAATGAAC
    ATGCCCCTCA ACTCAGATGG GACGGTGACA TTCAACGCCA CACTCTTTGC CCTGGTCCGG
    ACATCCCTGA AGATCAAAAC AGAAGGGAAC CTGGAGCAAG CCAACCAGGA GCTGCGGATT
    GTCATCAAAA AGATCTGGAA GCGGATGAAA CAGAAGCTGC TAGATGAGGT CATCCCCCCA
    CCAGACGAGG AGGAGGTCAC CGTGGGCAAA TTCTACGCCA CATTTCTGAT CCAGGACTAT
    TTCCGCAAAT TCCGGCGGAG GAAAGAAAAA GGGCTACTAG GCAACGACGC CGCCCCTAGC
    ACCTCTTCCG CCCTTCAGGC TGGTCTGCGG AGCCTGCAGG ACTTGGGTCC TGAGATGCGG
    CAGGCCCTCA CCTGTGACAC AGAGGAGGAG GAAGAAGAGG GGCAGGAGGG AGTGGAGGAG
    GAAGATGAAA AGGACTTGGA AACTAACAAA GCCACGATGG TCTCCCAGCC CTCAGCTCGC
    CGGGGCTCCG GGATTTCTGT GTCTCTGCCT GTCGGGGACA GACTTCCAGA TTCACTCTCC
    TTTGGGCCCA GTGATGATGA CAGGGGGACT CCCACCTCCA GTCAGCCCAG TGTGCCCCAG
    GCTGGATCCA ACACCCACAG GAGAGGCTCT GGGGCTCTCA TTTTCACCAT CCCAGAAGAA
    GGAAATTCTC AGCCCAAGGG AACCAAAGGG CAAAACAAGC AAGATGAGGA TGAGGAAGTC
    CCTGATCGGC TTTCCTACCT AGATGAGCAG GCAGGGACTC CCCCGTGCTC AGTCCTTTTG
    CCACCTCACA GAGCTCAGAG ATACATGGAT GGGCACCTGG TACCACGCCG CCGTCTGCTG
    CCCCCCACAC CTGCAGGTCG GAAGCCCTCC TTCACCATCC AGTGTCTGCA GCGCCAGGGC
    AGTTGTGAGG ATTTACCCAT CCCAGGCACC TATCATCGTG GGCGAAATTC AGGGCCCAAT
    AGGGCTCAGG GTTCCTGGGC AACACCACCT CAGCGGGGTC GGCTCCTGTA TGCCCCGCTG
    TTGTTGGTGG AAGAGGGCGC AGCGGGGGAG GGGTACCTCG GCAGATCCAG TGGCCCACTG
    CGCACCTTCA CCTGTCTGCA CGTGCCTGGA ACCCACTCGG ACCCCAGCCA TGGGAAGAGG
    GGCAGTGCCG ACAGCTTGGT GGAGGCTGTG CTTATCTCAG AGGGTCTGGG CCTCTTTGCT
    CGAGACCCAC GTTTCGTGGC CCTGGCCAAG CAGGAGATTG CAGATGCGTG TCGCCTGACG
    CTGGATGAGA TGGACAATGC TGCCAGTGAC CTGCTGGCAC AGGGAACCAG CTCTCTCTAT
    AGCGACGAGG AGTCCATCCT CTCCCGCTTC GATGAGGAGG ACTTGGGAGA CGAGATGGCC
    TGCGTCCACG CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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