cacna1f cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1f
Entrez Gene ID	100485906
Full Name	calcium channel, voltage-dependent, L type, alpha 1F subunit
Synonyms	ca(v)1.4,cav1.4
General protein information	Preferred Names calcium channel, voltage-dependent, L type, alpha 1F subunit Names voltage-dependent L-type calcium channel subunit alpha-1F
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031891455.1	XP_031747315.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
XM_012968727.2	XP_012824181.1	voltage-dependent L-type calcium channel subunit alpha-1F
XM_031891456.1	XP_031747316.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1F	NP_005174.2
	CACNA1F	XP_001106163.2
Canis lupus familiaris (dog)	CACNA1F	XP_005642146.1
Danio rerio (zebrafish)	LOC559964	XP_003199134.2
Bos taurus (cattle)	CACNA1F	NP_001192592.1
Mus musculus (house mouse)	Cacna1f	NP_062528.2
Danio rerio (zebrafish)	LOC793708	XP_001333514.5
Xenopus tropicalis (tropical clawed frog)	cacna1f	XP_002941018.2
Rattus norvegicus (Norway rat)	Cacna1f	NP_446153.1

Gene Ontology

The following cacna1f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa33472	XM_012968727.2 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa64765	XM_031891455.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64766	XM_031891456.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1f ( XM_012968727.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824181.1 Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), mRNA.

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CloneID	OXa33472
Clone ID Related Accession (Same CDS sequence)	XM_012968727.2
Accession Version	XM_012968727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent L-type calcium channel subunit alpha-1F
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT 
GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG 
AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC 
AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA 
GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG 
GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA 
GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC 
GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC 
ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT 
GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT 
GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC 
ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC 
ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC 
ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA 
AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA 
ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG 
GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG 
GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT 
GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA 
AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC 
CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCG CCTGTCTCTG 
GCTGACATCA CTGGCAGGAG GAAGCGGAGA CTTCAATGGC TTTTGCATGC TAACCATTCC 
ACAGAGACCC ACACTAGCCT TCCAGCAAGT GAGACCACTT CTGTGAACAC CGAGAATATA 
GACGAGGAGC ATGCTAACTG CTGTGCCCGC TGTTTGAGCA AGCTGTCACA GACTGGGTGC 
TGCCGTAAGA TAAGGCGGTG GAACCGAGCC TTTCGACGGA AATGCCGCCT GGCTGTTAAA 
TCGGTCACCT TCTACTGGAT GGTTCTGCTC CTCGTCTTCC TCAACACGCT GACCATTGCA 
TCAGAGCATT ACCAACAGCC AGATTGGCTG ACTGAGATTC AAGCGTATGC CAACAAGGTG 
CTTCTGTCTC TATTCACACT GGAGATGCTT CTCAAGATGT ACAGCCTTGG GTTACACGCC 
TACTTTGTGT CCTTCTTTAA CCGCTTTGAT TGCTTCGTGG TTTGTGGTGG GATCTTGGAG 
ACTGTCCTTG TAGAATTTGA CATCATGTCT CCTCTGGGAA TCTCTGTTCT TCGTTGTGTA 
CGACTATTGA GGATCTTTAA GGTCACCAGA CACTGGGCAT CACTAAGTAA TTTGGTGGCT 
TCCCTTTTGA ACTCCATGAA ATCCATTGCT TCTTTGCTGC TGCTTCTCTT CCTCTTCATT 
ATCATCTTCT CCTTGCTCGG AATGCAGCTC TTTGGGGGCA AATTCAACTT TGATGAGACC 
CAGACCAAGA GGAGCACATT TGACACCTTC CCTCAGGCCC TGCTTACTGT CTTCCAGATA 
CTCACAGGTG AGGACTGGAA TGCAGTCATG TACGATGGGA TAATGGCGTA TGGGGGACCA 
TTTTTCCCTG GAATGCTGGT CTGTGTGTAT TTTATCATCC TCTTCATATG TGGTAACTAT 
ATCCTACTCA ATGTCTTTTT GGCCATCGCT GTGGACAATC TGGCTGATGG TGACAACATC 
AATAGCAAAG ATAAAAAGGT GAACAACAAA TCAGAGAAGA AGGATGAAAA CTCTGTCAAG 
GTTCCGTGTG AGGAGGAGAA TGAAAACCAG GAGGAGGAAG GGAGTGAAGC CTCTGAAGAA 
GAGGAAGATG ATGAGGGCAG CCTCCCAGAA TCACACTTGG ATAAAATTGC TGACTTTGCT 
CCAAAGGAGA AGGTTCTTCC CATTCCCGAG GGCAGTGCAT TCTTCATCCT CAGTAACACC 
AACCTCCTCC GCGTTGGGTG CCACAAGCTG ATTCACCACC ACATTTTCAC CAACCTCATT 
TTAGTCTTCA TCATCCTCAG CAGCATTTCC CTGGCAGCCG AAGATCCAAT CCGCGCCCAC 
TCTTTTCGTA ATCATATCCT GGGGTATTTT GATTATGCAT TCACGTCTAT CTTCACAGTG 
GAGATTTTGC TGAAGATGAC AGCATATGGT GCATTCTTGC ACAAGGGCTC CTTCTGCAGG 
AACTGGTTCA ATCTGCTGGA TCTCCTTGTT GTCAGCGTGT CTCTCATCAG TTTTGGAATC 
CATTCTAGCG CTATCTCCGT TGTAAAGATT CTACGTGTTC TGAGAGTACT GCGTCCATTA 
AGAGCCATCA ACAGAGCCAA GGGGCTTAAG CACGTGGTCC AGTGCGTCTT TGTTGCAATC 
CGCACTATCG GTAACATTAT GATAGTGACT ACTTTGCTGC AGTTCATGTT CGCCTGCATA 
GGTGTTCAGC TTTTCAAGGG CAAGTTTTAC AGTTGCACTG ATGAGGCCAA ACGCAGCCCA 
GAGGAGTGCA AGTTACTATA CAAAGCCATT GATGCCTATG GGGAGAACGT AGGACCTATT 
TACAACTACC GTGTGGAGAT CTCCATCTTT TTTATTGTCT ACATTATCAT CATTGCCTTC 
TTCATGATGA ACATCTTTGT GGGTTTTGTG ATTATCACTT TCCGAGCACA AGGAGAGCAG 
GAGTACAAGA ACTGTGAACT GGACAAGAAC CAGCGCCAGT GTGTGGAATA TGCATTGAAG 
GCTCAGCCTC TGCGGCTGTA CATCCCCAAG GCAAAGTATC AGTACAAAGT CTGGTACATG 
GTCAACTCAA CTGGCTTTGA GTACATCATG TTTGTCCTTA TCCTGCTCAA CACTGTTGCT 
TTAGCCATGC AGCATTATGA GCAGTCCAAG CCCTTTAATT ATGCAATGGA TATCCTGAAT 
ATGGTCTTCA CTGGACTCTT CACCGTGGAA ATGGTGCTAA AGCTGATGGC ATTTCGACCC 
AAGCATTATT TCTGCGATGC CTGGAACACA TTTGATGCTC TCATTGTAGT GGGCAGCCTT 
GTGGATATTG CAGTCACCGA GGTCAATAAT GGATGGCATG TTGGTGAGAG TTCAGAGGAT 
AGCTCTCGTA TTTCCATCAC ATTCTTCCGG CTCTTCCGCG TCATGCGTTT GGTAAAGCTT 
CTTAGTAAAG GAGAGGGAAT CAGGACACTG CTGTGGACAT TCATCAAATC CTTCCAGGCT 
TTGCCATACG TTGCTCTGCT CATCGCTATG ATATTTTTTA TCTACGCTGT CATCGGCATG 
CAGACATTTG GGAAAATTGC AATGCAAGAT GCGAGTCAAA TCAACAGAAA TAACAACTTT 
CAGACCTTTC CTCAGGCTGT GCTTTTACTG TTCAGGTGTG CAACCGGGGA GGCCTGGCAA 
GACATCATGT TGGCAAGCCT GCCTGGGAAG CGGTGTGACA GCGAGTCCGA CTTTGGTCCT 
GGGGAGGAAT TTACCTGTGG GAGTAACTTC GCTATTGTGT ATTTCATCAG TTTCTTCATG 
CTCTGCGCCT TCCTTATCAT TAACCTGTTT GTGGCTGTCA TTATGGACAA CTTTGACTAC 
CTCACTAGGG ATTGGTCAAT TCTGGGCCCA CATCATTTAG ATGAGTTTAA GAGGATCTGG 
TCGGAATACG ATCCCGAAGC TAAGGGTCGC ATCAAGCACC TGGATGTTGT GACCTTGCTT 
CGCAGGATCC AGCCTCCACT GGGATTTGGG AAGCTGTGCC CCCATCGTGT AGCATGTAAG 
AGGCTGGTGG CCATGAATAT GCCTTTAAAT GCAGATGGGA CTGTCACCTT TAATGCTACA 
CTTTTTGCCC TTGTTCGAAC ATCACTCAAG ATTAAAACTG AAGGTAACCT AGAGACTGCC 
AATGAGGAAC TGAGAGCTGT CATCAAAAAG ATCTGGAAAA GAACCAAGCA GAAGATACTA 
GATGAGGTGA TTCCCCCTCC TGAAGAGGAG GAGGTGACGG TTGGGAAATT TTATGCCACA 
TTCCTGATCC AGGATTACTT TCGCAAGTTT CGCCGAAGAA AAGAAAAGGG GCTCCTGGGA 
ACAGAGAATT TGCCTTGTAA TTCCACAACA CTGCAGGCAG GGCTGAGGAG CCTACAGGAT 
CTTGGTTCAG AGATACGACG AGTGATATCC ACTGAGCTAG AGGAGGAAGA AGATATCTCT 
GGATTCAGAG CACTGGGGCT TCAGGTTTCA GAAGACAAAT CATTGTGCGA AAACTTAACT 
TTGGTGCAGG CAACTGAAAA TAAGATTTCT GGATCTGTAG TATCTCTTAC TAACTCCATT 
GGAGGAGGAA GAGAAGAGCA GCTACCAATG TCTCGAAGAT CATCACTAAT AAATCGGTCT 
GTAGGAGGAT CTAAGGACAG TTTGTTGGTC CCACCCACAC CACCACCCAA TGTGTCGTCC 
AGCATGAAGA GGAGGCAGAT GACCACACGG AGGAGAAGCT CAGAAGGCAG CGGTATCACT 
CCCACTGTGC ATGAAGAGGA AGAGGAGAGT GCAGCAGAAC ATGAGCTGAG ATCTAGACCT 
CCCTCACCGG GGTACGACCA AAGCAATGAG ATACAGCGCC AGTCTAGTTT GGAGGAGTCT 
CAGCAGCGCT CAGCATCTCC TCAGGAAAGA GTGAAAGTTC TGCCTCCTCC GGCTGCCCCT 
CCATCTAGTT TCAGAGCGAA TGGCACCTTG GAAGGAAGAG CAACCCAAAG AAGGCGCCTG 
CTGCCTCCTA CCCCAGGTAC CAAGTCTGCA TTTAGCATCC AGTGCCTGAA GAGACAGGGG 
AGCTGTGATG ATATACCCAT CCCAGGAACA TACCATCAGA ATGCACCCCC ATGCAGAACA 
AGAGGCCAGG TTTATGGAAG TACCGATTCT TGGCAAAGAA ATAGTTACTC CACCAATGGC 
ACTCACTCAT GGGCCAACCC ACCAAAGCGT GGGCGCCTTC TTTATGCACC CCTTATATTG 
GTGGAGGAAG ACAGCGCCCT GCCTACTATT CCATGCACTC GCTGGTATCA GGAAGAGGAT 
AGCAAAGCAC CCTATTGCCA CTACACCCAG CTGCGTGTGC CGGGCCAGAG GCTTGCCGAG 
AAGAGAGGCA GCGCTGACAG TCTAGTGGAG GCGGTTTTGA TATCAGAAGG CCTAGGGCTT 
TATGCACGGG ACCCCAAGTT TGTCGCTTTC GCCAAACGTG AGATCGCAGA TGCCTGTCAG 
ATGACCATTG ATGAGATGGA GAGTGCCGCC AGTGACTTGC TAAGCCAGAG CAGGAAAGGC 
AGTTGGACTG GAAATAATGG ACACTCCTAC CAAGATGAAA CGGCATGGAA TGGACTTTTG 
TACAGTGATG AAGACTCTAT ACACTGCCGA GAAGAAGAGG AATTAAGAGA TGAGATGGCC 
TGCGTGTCTT CATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824181.1
CDS	286..6243
Translation	MSEPEENDKGPAPPYSSEEAEPFMASAEAALACWRYSMDGGPMMNGTDTLNSTASSQAQKKRQYHSKHKNQGTGGVHRSPRALFCLYLSNPIRRACISIVEWKPFDILILMTIFANCVALGVYIPFPEDDSNIANHNLEQVEYIFLIIFTVESFLKIIAYGLVLHPSAYIRNGWNLLDFVIVVIGLFSVILEQFSHKQGDAHHMSGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKAMVPLLHIALLVLFVIIIYAIIGLELFIGRMHKTCFYIGSDLVSEEEPAPCAFSGHGRECLLNNTECRGKWEGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLKGYLDWITQAEDLEPDQEIRDERHRLSLADITGRRKRRLQWLLHANHSTETHTSLPASETTSVNTENIDEEHANCCARCLSKLSQTGCCRKIRRWNRAFRRKCRLAVKSVTFYWMVLLLVFLNTLTIASEHYQQPDWLTEIQAYANKVLLSLFTLEMLLKMYSLGLHAYFVSFFNRFDCFVVCGGILETVLVEFDIMSPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDTFPQALLTVFQILTGEDWNAVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLADGDNINSKDKKVNNKSEKKDENSVKVPCEEENENQEEEGSEASEEEEDDEGSLPESHLDKIADFAPKEKVLPIPEGSAFFILSNTNLLRVGCHKLIHHHIFTNLILVFIILSSISLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTAYGAFLHKGSFCRNWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKRSPEECKLLYKAIDAYGENVGPIYNYRVEISIFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYKNCELDKNQRQCVEYALKAQPLRLYIPKAKYQYKVWYMVNSTGFEYIMFVLILLNTVALAMQHYEQSKPFNYAMDILNMVFTGLFTVEMVLKLMAFRPKHYFCDAWNTFDALIVVGSLVDIAVTEVNNGWHVGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQTFGKIAMQDASQINRNNNFQTFPQAVLLLFRCATGEAWQDIMLASLPGKRCDSESDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNADGTVTFNATLFALVRTSLKIKTEGNLETANEELRAVIKKIWKRTKQKILDEVIPPPEEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGTENLPCNSTTLQAGLRSLQDLGSEIRRVISTELEEEEDISGFRALGLQVSEDKSLCENLTLVQATENKISGSVVSLTNSIGGGREEQLPMSRRSSLINRSVGGSKDSLLVPPTPPPNVSSSMKRRQMTTRRRSSEGSGITPTVHEEEEESAAEHELRSRPPSPGYDQSNEIQRQSSLEESQQRSASPQERVKVLPPPAAPPSSFRANGTLEGRATQRRRLLPPTPGTKSAFSIQCLKRQGSCDDIPIPGTYHQNAPPCRTRGQVYGSTDSWQRNSYSTNGTHSWANPPKRGRLLYAPLILVEEDSALPTIPCTRWYQEEDSKAPYCHYTQLRVPGQRLAEKRGSADSLVEAVLISEGLGLYARDPKFVAFAKREIADACQMTIDEMESAASDLLSQSRKGSWTGNNGHSYQDETAWNGLLYSDEDSIHCREEEELRDEMACVSSF

Target ORF information:

RefSeq Version	XM_012968727.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968727.2

ORF Insert Sequence:

ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT 
GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG 
AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC 
AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA 
GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG 
GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA 
GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC 
GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC 
ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT 
GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT 
GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC 
ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC 
ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC 
ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA 
AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA 
ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG 
GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG 
GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT 
GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA 
AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC 
CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCG CCTGTCTCTG 
GCTGACATCA CTGGCAGGAG GAAGCGGAGA CTTCAATGGC TTTTGCATGC TAACCATTCC 
ACAGAGACCC ACACTAGCCT TCCAGCAAGT GAGACCACTT CTGTGAACAC CGAGAATATA 
GACGAGGAGC ATGCTAACTG CTGTGCCCGC TGTTTGAGCA AGCTGTCACA GACTGGGTGC 
TGCCGTAAGA TAAGGCGGTG GAACCGAGCC TTTCGACGGA AATGCCGCCT GGCTGTTAAA 
TCGGTCACCT TCTACTGGAT GGTTCTGCTC CTCGTCTTCC TCAACACGCT GACCATTGCA 
TCAGAGCATT ACCAACAGCC AGATTGGCTG ACTGAGATTC AAGCGTATGC CAACAAGGTG 
CTTCTGTCTC TATTCACACT GGAGATGCTT CTCAAGATGT ACAGCCTTGG GTTACACGCC 
TACTTTGTGT CCTTCTTTAA CCGCTTTGAT TGCTTCGTGG TTTGTGGTGG GATCTTGGAG 
ACTGTCCTTG TAGAATTTGA CATCATGTCT CCTCTGGGAA TCTCTGTTCT TCGTTGTGTA 
CGACTATTGA GGATCTTTAA GGTCACCAGA CACTGGGCAT CACTAAGTAA TTTGGTGGCT 
TCCCTTTTGA ACTCCATGAA ATCCATTGCT TCTTTGCTGC TGCTTCTCTT CCTCTTCATT 
ATCATCTTCT CCTTGCTCGG AATGCAGCTC TTTGGGGGCA AATTCAACTT TGATGAGACC 
CAGACCAAGA GGAGCACATT TGACACCTTC CCTCAGGCCC TGCTTACTGT CTTCCAGATA 
CTCACAGGTG AGGACTGGAA TGCAGTCATG TACGATGGGA TAATGGCGTA TGGGGGACCA 
TTTTTCCCTG GAATGCTGGT CTGTGTGTAT TTTATCATCC TCTTCATATG TGGTAACTAT 
ATCCTACTCA ATGTCTTTTT GGCCATCGCT GTGGACAATC TGGCTGATGG TGACAACATC 
AATAGCAAAG ATAAAAAGGT GAACAACAAA TCAGAGAAGA AGGATGAAAA CTCTGTCAAG 
GTTCCGTGTG AGGAGGAGAA TGAAAACCAG GAGGAGGAAG GGAGTGAAGC CTCTGAAGAA 
GAGGAAGATG ATGAGGGCAG CCTCCCAGAA TCACACTTGG ATAAAATTGC TGACTTTGCT 
CCAAAGGAGA AGGTTCTTCC CATTCCCGAG GGCAGTGCAT TCTTCATCCT CAGTAACACC 
AACCTCCTCC GCGTTGGGTG CCACAAGCTG ATTCACCACC ACATTTTCAC CAACCTCATT 
TTAGTCTTCA TCATCCTCAG CAGCATTTCC CTGGCAGCCG AAGATCCAAT CCGCGCCCAC 
TCTTTTCGTA ATCATATCCT GGGGTATTTT GATTATGCAT TCACGTCTAT CTTCACAGTG 
GAGATTTTGC TGAAGATGAC AGCATATGGT GCATTCTTGC ACAAGGGCTC CTTCTGCAGG 
AACTGGTTCA ATCTGCTGGA TCTCCTTGTT GTCAGCGTGT CTCTCATCAG TTTTGGAATC 
CATTCTAGCG CTATCTCCGT TGTAAAGATT CTACGTGTTC TGAGAGTACT GCGTCCATTA 
AGAGCCATCA ACAGAGCCAA GGGGCTTAAG CACGTGGTCC AGTGCGTCTT TGTTGCAATC 
CGCACTATCG GTAACATTAT GATAGTGACT ACTTTGCTGC AGTTCATGTT CGCCTGCATA 
GGTGTTCAGC TTTTCAAGGG CAAGTTTTAC AGTTGCACTG ATGAGGCCAA ACGCAGCCCA 
GAGGAGTGCA AGTTACTATA CAAAGCCATT GATGCCTATG GGGAGAACGT AGGACCTATT 
TACAACTACC GTGTGGAGAT CTCCATCTTT TTTATTGTCT ACATTATCAT CATTGCCTTC 
TTCATGATGA ACATCTTTGT GGGTTTTGTG ATTATCACTT TCCGAGCACA AGGAGAGCAG 
GAGTACAAGA ACTGTGAACT GGACAAGAAC CAGCGCCAGT GTGTGGAATA TGCATTGAAG 
GCTCAGCCTC TGCGGCTGTA CATCCCCAAG GCAAAGTATC AGTACAAAGT CTGGTACATG 
GTCAACTCAA CTGGCTTTGA GTACATCATG TTTGTCCTTA TCCTGCTCAA CACTGTTGCT 
TTAGCCATGC AGCATTATGA GCAGTCCAAG CCCTTTAATT ATGCAATGGA TATCCTGAAT 
ATGGTCTTCA CTGGACTCTT CACCGTGGAA ATGGTGCTAA AGCTGATGGC ATTTCGACCC 
AAGCATTATT TCTGCGATGC CTGGAACACA TTTGATGCTC TCATTGTAGT GGGCAGCCTT 
GTGGATATTG CAGTCACCGA GGTCAATAAT GGATGGCATG TTGGTGAGAG TTCAGAGGAT 
AGCTCTCGTA TTTCCATCAC ATTCTTCCGG CTCTTCCGCG TCATGCGTTT GGTAAAGCTT 
CTTAGTAAAG GAGAGGGAAT CAGGACACTG CTGTGGACAT TCATCAAATC CTTCCAGGCT 
TTGCCATACG TTGCTCTGCT CATCGCTATG ATATTTTTTA TCTACGCTGT CATCGGCATG 
CAGACATTTG GGAAAATTGC AATGCAAGAT GCGAGTCAAA TCAACAGAAA TAACAACTTT 
CAGACCTTTC CTCAGGCTGT GCTTTTACTG TTCAGGTGTG CAACCGGGGA GGCCTGGCAA 
GACATCATGT TGGCAAGCCT GCCTGGGAAG CGGTGTGACA GCGAGTCCGA CTTTGGTCCT 
GGGGAGGAAT TTACCTGTGG GAGTAACTTC GCTATTGTGT ATTTCATCAG TTTCTTCATG 
CTCTGCGCCT TCCTTATCAT TAACCTGTTT GTGGCTGTCA TTATGGACAA CTTTGACTAC 
CTCACTAGGG ATTGGTCAAT TCTGGGCCCA CATCATTTAG ATGAGTTTAA GAGGATCTGG 
TCGGAATACG ATCCCGAAGC TAAGGGTCGC ATCAAGCACC TGGATGTTGT GACCTTGCTT 
CGCAGGATCC AGCCTCCACT GGGATTTGGG AAGCTGTGCC CCCATCGTGT AGCATGTAAG 
AGGCTGGTGG CCATGAATAT GCCTTTAAAT GCAGATGGGA CTGTCACCTT TAATGCTACA 
CTTTTTGCCC TTGTTCGAAC ATCACTCAAG ATTAAAACTG AAGGTAACCT AGAGACTGCC 
AATGAGGAAC TGAGAGCTGT CATCAAAAAG ATCTGGAAAA GAACCAAGCA GAAGATACTA 
GATGAGGTGA TTCCCCCTCC TGAAGAGGAG GAGGTGACGG TTGGGAAATT TTATGCCACA 
TTCCTGATCC AGGATTACTT TCGCAAGTTT CGCCGAAGAA AAGAAAAGGG GCTCCTGGGA 
ACAGAGAATT TGCCTTGTAA TTCCACAACA CTGCAGGCAG GGCTGAGGAG CCTACAGGAT 
CTTGGTTCAG AGATACGACG AGTGATATCC ACTGAGCTAG AGGAGGAAGA AGATATCTCT 
GGATTCAGAG CACTGGGGCT TCAGGTTTCA GAAGACAAAT CATTGTGCGA AAACTTAACT 
TTGGTGCAGG CAACTGAAAA TAAGATTTCT GGATCTGTAG TATCTCTTAC TAACTCCATT 
GGAGGAGGAA GAGAAGAGCA GCTACCAATG TCTCGAAGAT CATCACTAAT AAATCGGTCT 
GTAGGAGGAT CTAAGGACAG TTTGTTGGTC CCACCCACAC CACCACCCAA TGTGTCGTCC 
AGCATGAAGA GGAGGCAGAT GACCACACGG AGGAGAAGCT CAGAAGGCAG CGGTATCACT 
CCCACTGTGC ATGAAGAGGA AGAGGAGAGT GCAGCAGAAC ATGAGCTGAG ATCTAGACCT 
CCCTCACCGG GGTACGACCA AAGCAATGAG ATACAGCGCC AGTCTAGTTT GGAGGAGTCT 
CAGCAGCGCT CAGCATCTCC TCAGGAAAGA GTGAAAGTTC TGCCTCCTCC GGCTGCCCCT 
CCATCTAGTT TCAGAGCGAA TGGCACCTTG GAAGGAAGAG CAACCCAAAG AAGGCGCCTG 
CTGCCTCCTA CCCCAGGTAC CAAGTCTGCA TTTAGCATCC AGTGCCTGAA GAGACAGGGG 
AGCTGTGATG ATATACCCAT CCCAGGAACA TACCATCAGA ATGCACCCCC ATGCAGAACA 
AGAGGCCAGG TTTATGGAAG TACCGATTCT TGGCAAAGAA ATAGTTACTC CACCAATGGC 
ACTCACTCAT GGGCCAACCC ACCAAAGCGT GGGCGCCTTC TTTATGCACC CCTTATATTG 
GTGGAGGAAG ACAGCGCCCT GCCTACTATT CCATGCACTC GCTGGTATCA GGAAGAGGAT 
AGCAAAGCAC CCTATTGCCA CTACACCCAG CTGCGTGTGC CGGGCCAGAG GCTTGCCGAG 
AAGAGAGGCA GCGCTGACAG TCTAGTGGAG GCGGTTTTGA TATCAGAAGG CCTAGGGCTT 
TATGCACGGG ACCCCAAGTT TGTCGCTTTC GCCAAACGTG AGATCGCAGA TGCCTGTCAG 
ATGACCATTG ATGAGATGGA GAGTGCCGCC AGTGACTTGC TAAGCCAGAG CAGGAAAGGC 
AGTTGGACTG GAAATAATGG ACACTCCTAC CAAGATGAAA CGGCATGGAA TGGACTTTTG 
TACAGTGATG AAGACTCTAT ACACTGCCGA GAAGAAGAGG AATTAAGAGA TGAGATGGCC 
TGCGTGTCTT CATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1f ( XM_031891455.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747315.1 Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X1, mRNA.

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CloneID	OXa64765
Clone ID Related Accession (Same CDS sequence)	XM_031891455.1
Accession Version	XM_031891455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT 
GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG 
AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC 
AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA 
GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG 
GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA 
GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC 
GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC 
ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT 
GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT 
GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC 
ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC 
ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC 
ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA 
AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA 
ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG 
GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG 
GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT 
GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA 
AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC 
CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCG CCTGTCTCTG 
GCTGACATCA CTGGCAGGAG GAAGCGGAGA CTTCAATGGC TTTTGCATGC TAACCATTCC 
ACAGAGACCC ACACTAGCCT TCCAGCAAGT GAGACCACTT CTGTGAACAC CGAGAATATA 
GACGAGGAGC ATGCTAACTG CTGTGCCCGC TGTTTGAGCA AGCTGTCACA GACTGGGTGC 
TGCCGTAAGA TAAGGCGGTG GAACCGAGCC TTTCGACGGA AATGCCGCCT GGCTGTTAAA 
TCGGTCACCT TCTACTGGAT GGTTCTGCTC CTCGTCTTCC TCAACACGCT GACCATTGCA 
TCAGAGCATT ACCAACAGCC AGATTGGCTG ACTGAGATTC AAGCGTATGC CAACAAGGTG 
CTTCTGTCTC TATTCACACT GGAGATGCTT CTCAAGATGT ACAGCCTTGG GTTACACGCC 
TACTTTGTGT CCTTCTTTAA CCGCTTTGAT TGCTTCGTGG TTTGTGGTGG GATCTTGGAG 
ACTGTCCTTG TAGAATTTGA CATCATGTCT CCTCTGGGAA TCTCTGTTCT TCGTTGTGTA 
CGACTATTGA GGATCTTTAA GGTCACCAGA CACTGGGCAT CACTAAGTAA TTTGGTGGCT 
TCCCTTTTGA ACTCCATGAA ATCCATTGCT TCTTTGCTGC TGCTTCTCTT CCTCTTCATT 
ATCATCTTCT CCTTGCTCGG AATGCAGCTC TTTGGGGGCA AATTCAACTT TGATGAGACC 
CAGACCAAGA GGAGCACATT TGACACCTTC CCTCAGGCCC TGCTTACTGT CTTCCAGATA 
CTCACAGGTG AGGACTGGAA TGCAGTCATG TACGATGGGA TAATGGCGTA TGGGGGACCA 
TTTTTCCCTG GAATGCTGGT CTGTGTGTAT TTTATCATCC TCTTCATATG TGGTAACTAT 
ATCCTACTCA ATGTCTTTTT GGCCATCGCT GTGGACAATC TGGCTGATGG TGACAACATC 
AATAGCAAAG ATAAAAAGGT GAACAACAAA TCAGAGAAGA AGGATGAAAA CTCTGTCAAG 
GTTCCGTGTG AGGAGGAGAA TGAAAACCAG GAGGAGGAAG GGAGTGAAGC CTCTGAAGAA 
GAGGAAGATG ATGAGGGCAG CCTCCCAGAA TCACACTTGG ATAAAATTGC TGACTTTGCT 
CCAAAGGAGA AGGTTCTTCC CATTCCCGAG GGCAGTGCAT TCTTCATCCT CAGTAACACC 
AACCTCCTCC GCGTTGGGTG CCACAAGCTG ATTCACCACC ACATTTTCAC CAACCTCATT 
TTAGTCTTCA TCATCCTCAG CAGCATTTCC CTGGCAGCCG AAGATCCAAT CCGCGCCCAC 
TCTTTTCGTA ATCATATCCT GGGGTATTTT GATTATGCAT TCACGTCTAT CTTCACAGTG 
GAGATTTTGC TGAAGATGAC AGCATATGGT GCATTCTTGC ACAAGGGCTC CTTCTGCAGG 
AACTGGTTCA ATCTGCTGGA TCTCCTTGTT GTCAGCGTGT CTCTCATCAG TTTTGGAATC 
CATTCTAGCG CTATCTCCGT TGTAAAGATT CTACGTGTTC TGAGAGTACT GCGTCCATTA 
AGAGCCATCA ACAGAGCCAA GGGGCTTAAG CACGTGGTCC AGTGCGTCTT TGTTGCAATC 
CGCACTATCG GTAACATTAT GATAGTGACT ACTTTGCTGC AGTTCATGTT CGCCTGCATA 
GGTGTTCAGC TTTTCAAGGG CAAGTTTTAC AGTTGCACTG ATGAGGCCAA ACGCAGCCCA 
GAGGAGTGCA AGGGCACATT CATTATTTAT AAGGAAGGGG ACGTTTCACA CCCCATGGTG 
CGGGAAAGAA TTTGGCACAA CAGTGACTTC AACTTTGACA ACGTCCTGTC AGGCATGATG 
GCTCTGTTTA CAGTGTCCAC ATTTGAAGGT TGGCCAGCGT TACTATACAA AGCCATTGAT 
GCCTATGGGG AGAACGTAGG ACCTATTTAC AACTACCGTG TGGAGATCTC CATCTTTTTT 
ATTGTCTACA TTATCATCAT TGCCTTCTTC ATGATGAACA TCTTTGTGGG TTTTGTGATT 
ATCACTTTCC GAGCACAAGG AGAGCAGGAG TACAAGAACT GTGAACTGGA CAAGAACCAG 
CGCCAGTGTG TGGAATATGC ATTGAAGGCT CAGCCTCTGC GGCTGTACAT CCCCAAGGCA 
AAGTATCAGT ACAAAGTCTG GTACATGGTC AACTCAACTG GCTTTGAGTA CATCATGTTT 
GTCCTTATCC TGCTCAACAC TGTTGCTTTA GCCATGCAGC ATTATGAGCA GTCCAAGCCC 
TTTAATTATG CAATGGATAT CCTGAATATG GTCTTCACTG GACTCTTCAC CGTGGAAATG 
GTGCTAAAGC TGATGGCATT TCGACCCAAG CATTATTTCT GCGATGCCTG GAACACATTT 
GATGCTCTCA TTGTAGTGGG CAGCCTTGTG GATATTGCAG TCACCGAGGT CAATAATGGA 
TGGCATGTTG GTGAGAGTTC AGAGGATAGC TCTCGTATTT CCATCACATT CTTCCGGCTC 
TTCCGCGTCA TGCGTTTGGT AAAGCTTCTT AGTAAAGGAG AGGGAATCAG GACACTGCTG 
TGGACATTCA TCAAATCCTT CCAGGCTTTG CCATACGTTG CTCTGCTCAT CGCTATGATA 
TTTTTTATCT ACGCTGTCAT CGGCATGCAG ACATTTGGGA AAATTGCAAT GCAAGATGCG 
AGTCAAATCA ACAGAAATAA CAACTTTCAG ACCTTTCCTC AGGCTGTGCT TTTACTGTTC 
AGGTGTGCAA CCGGGGAGGC CTGGCAAGAC ATCATGTTGG CAAGCCTGCC TGGGAAGCGG 
TGTGACAGCG AGTCCGACTT TGGTCCTGGG GAGGAATTTA CCTGTGGGAG TAACTTCGCT 
ATTGTGTATT TCATCAGTTT CTTCATGCTC TGCGCCTTCC TTATCATTAA CCTGTTTGTG 
GCTGTCATTA TGGACAACTT TGACTACCTC ACTAGGGATT GGTCAATTCT GGGCCCACAT 
CATTTAGATG AGTTTAAGAG GATCTGGTCG GAATACGATC CCGAAGCTAA GGGTCGCATC 
AAGCACCTGG ATGTTGTGAC CTTGCTTCGC AGGATCCAGC CTCCACTGGG ATTTGGGAAG 
CTGTGCCCCC ATCGTGTAGC ATGTAAGAGG CTGGTGGCCA TGAATATGCC TTTAAATGCA 
GATGGGACTG TCACCTTTAA TGCTACACTT TTTGCCCTTG TTCGAACATC ACTCAAGATT 
AAAACTGAAG GTAACCTAGA GACTGCCAAT GAGGAACTGA GAGCTGTCAT CAAAAAGATC 
TGGAAAAGAA CCAAGCAGAA GATACTAGAT GAGGTGATTC CCCCTCCTGA AGAGGAGGAG 
GTGACGGTTG GGAAATTTTA TGCCACATTC CTGATCCAGG ATTACTTTCG CAAGTTTCGC 
CGAAGAAAAG AAAAGGGGCT CCTGGGAACA GAGAATTTGC CTTGTAATTC CACAACACTG 
CAGGCAGGGC TGAGGAGCCT ACAGGATCTT GGTTCAGAGA TACGACGAGT GATATCCACT 
GAGCTAGAGG AGGAAGAAGA TATCTCTGGA TTCAGAGCAC TGGGGCTTCA GGTTTCAGAA 
GACAAATCAT TGTGCGAAAA CTTAACTTTG GTGCAGGCAA CTGAAAATAA GATTTCTGGA 
TCTGTAGTAT CTCTTACTAA CTCCATTGGA GGAGGAAGAG AAGAGCAGCT ACCAATGTCT 
CGAAGATCAT CACTAATAAA TCGGTCTGTA GGAGGATCTA AGGACAGTTT GTTGGTCCCA 
CCCACACCAC CACCCAATGT GTCGTCCAGC ATGAAGAGGA GGCAGATGAC CACACGGAGG 
AGAAGCTCAG AAGGCAGCGG TATCACTCCC ACTGTGCATG AAGAGGAAGA GGAGAGTGCA 
GCAGAACATG AGCTGAGATC TAGACCTCCC TCACCGGGGT ACGACCAAAG CAATGAGATA 
CAGCGCCAGT CTAGTTTGGA GGAGTCTCAG CAGCGCTCAG CATCTCCTCA GGAAAGAGTG 
AAAGTTCTGC CTCCTCCGGC TGCCCCTCCA TCTAGTTTCA GAGCGAATGG CACCTTGGAA 
GGAAGAGCAA CCCAAAGAAG GCGCCTGCTG CCTCCTACCC CAGGTACCAA GTCTGCATTT 
AGCATCCAGT GCCTGAAGAG ACAGGGGAGC TGTGATGATA TACCCATCCC AGGAACATAC 
CATCAGAATG CACCCCCATG CAGAACAAGA GGCCAGGTTT ATGGAAGTAC CGATTCTTGG 
CAAAGAAATA GTTACTCCAC CAATGGCACT CACTCATGGG CCAACCCACC AAAGCGTGGG 
CGCCTTCTTT ATGCACCCCT TATATTGGTG GAGGAAGACA GCGCCCTGCC TACTATTCCA 
TGCACTCGCT GGTATCAGGA AGAGGATAGC AAAGCACCCT ATTGCCACTA CACCCAGCTG 
CGTGTGCCGG GCCAGAGGCT TGCCGAGAAG AGAGGCAGCG CTGACAGTCT AGTGGAGGCG 
GTTTTGATAT CAGAAGGCCT AGGGCTTTAT GCACGGGACC CCAAGTTTGT CGCTTTCGCC 
AAACGTGAGA TCGCAGATGC CTGTCAGATG ACCATTGATG AGATGGAGAG TGCCGCCAGT 
GACTTGCTAA GCCAGAGCAG GAAAGGCAGT TGGACTGGAA ATAATGGACA CTCCTACCAA 
GATGAAACGG CATGGAATGG ACTTTTGTAC AGTGATGAAG ACTCTATACA CTGCCGAGAA 
GAAGAGGAAT TAAGAGATGA GATGGCCTGC GTGTCTTCAT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747315.1
CDS	348..6452
Translation	MSEPEENDKGPAPPYSSEEAEPFMASAEAALACWRYSMDGGPMMNGTDTLNSTASSQAQKKRQYHSKHKNQGTGGVHRSPRALFCLYLSNPIRRACISIVEWKPFDILILMTIFANCVALGVYIPFPEDDSNIANHNLEQVEYIFLIIFTVESFLKIIAYGLVLHPSAYIRNGWNLLDFVIVVIGLFSVILEQFSHKQGDAHHMSGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKAMVPLLHIALLVLFVIIIYAIIGLELFIGRMHKTCFYIGSDLVSEEEPAPCAFSGHGRECLLNNTECRGKWEGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLKGYLDWITQAEDLEPDQEIRDERHRLSLADITGRRKRRLQWLLHANHSTETHTSLPASETTSVNTENIDEEHANCCARCLSKLSQTGCCRKIRRWNRAFRRKCRLAVKSVTFYWMVLLLVFLNTLTIASEHYQQPDWLTEIQAYANKVLLSLFTLEMLLKMYSLGLHAYFVSFFNRFDCFVVCGGILETVLVEFDIMSPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDTFPQALLTVFQILTGEDWNAVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLADGDNINSKDKKVNNKSEKKDENSVKVPCEEENENQEEEGSEASEEEEDDEGSLPESHLDKIADFAPKEKVLPIPEGSAFFILSNTNLLRVGCHKLIHHHIFTNLILVFIILSSISLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTAYGAFLHKGSFCRNWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKRSPEECKGTFIIYKEGDVSHPMVRERIWHNSDFNFDNVLSGMMALFTVSTFEGWPALLYKAIDAYGENVGPIYNYRVEISIFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYKNCELDKNQRQCVEYALKAQPLRLYIPKAKYQYKVWYMVNSTGFEYIMFVLILLNTVALAMQHYEQSKPFNYAMDILNMVFTGLFTVEMVLKLMAFRPKHYFCDAWNTFDALIVVGSLVDIAVTEVNNGWHVGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQTFGKIAMQDASQINRNNNFQTFPQAVLLLFRCATGEAWQDIMLASLPGKRCDSESDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNADGTVTFNATLFALVRTSLKIKTEGNLETANEELRAVIKKIWKRTKQKILDEVIPPPEEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGTENLPCNSTTLQAGLRSLQDLGSEIRRVISTELEEEEDISGFRALGLQVSEDKSLCENLTLVQATENKISGSVVSLTNSIGGGREEQLPMSRRSSLINRSVGGSKDSLLVPPTPPPNVSSSMKRRQMTTRRRSSEGSGITPTVHEEEEESAAEHELRSRPPSPGYDQSNEIQRQSSLEESQQRSASPQERVKVLPPPAAPPSSFRANGTLEGRATQRRRLLPPTPGTKSAFSIQCLKRQGSCDDIPIPGTYHQNAPPCRTRGQVYGSTDSWQRNSYSTNGTHSWANPPKRGRLLYAPLILVEEDSALPTIPCTRWYQEEDSKAPYCHYTQLRVPGQRLAEKRGSADSLVEAVLISEGLGLYARDPKFVAFAKREIADACQMTIDEMESAASDLLSQSRKGSWTGNNGHSYQDETAWNGLLYSDEDSIHCREEEELRDEMACVSSF

Target ORF information:

RefSeq Version	XM_031891455.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891455.1

ORF Insert Sequence:

ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT 
GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG 
AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC 
AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA 
GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG 
GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA 
GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC 
GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC 
ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT 
GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT 
GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC 
ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC 
ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC 
ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA 
AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA 
ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG 
GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG 
GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT 
GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA 
AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC 
CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCG CCTGTCTCTG 
GCTGACATCA CTGGCAGGAG GAAGCGGAGA CTTCAATGGC TTTTGCATGC TAACCATTCC 
ACAGAGACCC ACACTAGCCT TCCAGCAAGT GAGACCACTT CTGTGAACAC CGAGAATATA 
GACGAGGAGC ATGCTAACTG CTGTGCCCGC TGTTTGAGCA AGCTGTCACA GACTGGGTGC 
TGCCGTAAGA TAAGGCGGTG GAACCGAGCC TTTCGACGGA AATGCCGCCT GGCTGTTAAA 
TCGGTCACCT TCTACTGGAT GGTTCTGCTC CTCGTCTTCC TCAACACGCT GACCATTGCA 
TCAGAGCATT ACCAACAGCC AGATTGGCTG ACTGAGATTC AAGCGTATGC CAACAAGGTG 
CTTCTGTCTC TATTCACACT GGAGATGCTT CTCAAGATGT ACAGCCTTGG GTTACACGCC 
TACTTTGTGT CCTTCTTTAA CCGCTTTGAT TGCTTCGTGG TTTGTGGTGG GATCTTGGAG 
ACTGTCCTTG TAGAATTTGA CATCATGTCT CCTCTGGGAA TCTCTGTTCT TCGTTGTGTA 
CGACTATTGA GGATCTTTAA GGTCACCAGA CACTGGGCAT CACTAAGTAA TTTGGTGGCT 
TCCCTTTTGA ACTCCATGAA ATCCATTGCT TCTTTGCTGC TGCTTCTCTT CCTCTTCATT 
ATCATCTTCT CCTTGCTCGG AATGCAGCTC TTTGGGGGCA AATTCAACTT TGATGAGACC 
CAGACCAAGA GGAGCACATT TGACACCTTC CCTCAGGCCC TGCTTACTGT CTTCCAGATA 
CTCACAGGTG AGGACTGGAA TGCAGTCATG TACGATGGGA TAATGGCGTA TGGGGGACCA 
TTTTTCCCTG GAATGCTGGT CTGTGTGTAT TTTATCATCC TCTTCATATG TGGTAACTAT 
ATCCTACTCA ATGTCTTTTT GGCCATCGCT GTGGACAATC TGGCTGATGG TGACAACATC 
AATAGCAAAG ATAAAAAGGT GAACAACAAA TCAGAGAAGA AGGATGAAAA CTCTGTCAAG 
GTTCCGTGTG AGGAGGAGAA TGAAAACCAG GAGGAGGAAG GGAGTGAAGC CTCTGAAGAA 
GAGGAAGATG ATGAGGGCAG CCTCCCAGAA TCACACTTGG ATAAAATTGC TGACTTTGCT 
CCAAAGGAGA AGGTTCTTCC CATTCCCGAG GGCAGTGCAT TCTTCATCCT CAGTAACACC 
AACCTCCTCC GCGTTGGGTG CCACAAGCTG ATTCACCACC ACATTTTCAC CAACCTCATT 
TTAGTCTTCA TCATCCTCAG CAGCATTTCC CTGGCAGCCG AAGATCCAAT CCGCGCCCAC 
TCTTTTCGTA ATCATATCCT GGGGTATTTT GATTATGCAT TCACGTCTAT CTTCACAGTG 
GAGATTTTGC TGAAGATGAC AGCATATGGT GCATTCTTGC ACAAGGGCTC CTTCTGCAGG 
AACTGGTTCA ATCTGCTGGA TCTCCTTGTT GTCAGCGTGT CTCTCATCAG TTTTGGAATC 
CATTCTAGCG CTATCTCCGT TGTAAAGATT CTACGTGTTC TGAGAGTACT GCGTCCATTA 
AGAGCCATCA ACAGAGCCAA GGGGCTTAAG CACGTGGTCC AGTGCGTCTT TGTTGCAATC 
CGCACTATCG GTAACATTAT GATAGTGACT ACTTTGCTGC AGTTCATGTT CGCCTGCATA 
GGTGTTCAGC TTTTCAAGGG CAAGTTTTAC AGTTGCACTG ATGAGGCCAA ACGCAGCCCA 
GAGGAGTGCA AGGGCACATT CATTATTTAT AAGGAAGGGG ACGTTTCACA CCCCATGGTG 
CGGGAAAGAA TTTGGCACAA CAGTGACTTC AACTTTGACA ACGTCCTGTC AGGCATGATG 
GCTCTGTTTA CAGTGTCCAC ATTTGAAGGT TGGCCAGCGT TACTATACAA AGCCATTGAT 
GCCTATGGGG AGAACGTAGG ACCTATTTAC AACTACCGTG TGGAGATCTC CATCTTTTTT 
ATTGTCTACA TTATCATCAT TGCCTTCTTC ATGATGAACA TCTTTGTGGG TTTTGTGATT 
ATCACTTTCC GAGCACAAGG AGAGCAGGAG TACAAGAACT GTGAACTGGA CAAGAACCAG 
CGCCAGTGTG TGGAATATGC ATTGAAGGCT CAGCCTCTGC GGCTGTACAT CCCCAAGGCA 
AAGTATCAGT ACAAAGTCTG GTACATGGTC AACTCAACTG GCTTTGAGTA CATCATGTTT 
GTCCTTATCC TGCTCAACAC TGTTGCTTTA GCCATGCAGC ATTATGAGCA GTCCAAGCCC 
TTTAATTATG CAATGGATAT CCTGAATATG GTCTTCACTG GACTCTTCAC CGTGGAAATG 
GTGCTAAAGC TGATGGCATT TCGACCCAAG CATTATTTCT GCGATGCCTG GAACACATTT 
GATGCTCTCA TTGTAGTGGG CAGCCTTGTG GATATTGCAG TCACCGAGGT CAATAATGGA 
TGGCATGTTG GTGAGAGTTC AGAGGATAGC TCTCGTATTT CCATCACATT CTTCCGGCTC 
TTCCGCGTCA TGCGTTTGGT AAAGCTTCTT AGTAAAGGAG AGGGAATCAG GACACTGCTG 
TGGACATTCA TCAAATCCTT CCAGGCTTTG CCATACGTTG CTCTGCTCAT CGCTATGATA 
TTTTTTATCT ACGCTGTCAT CGGCATGCAG ACATTTGGGA AAATTGCAAT GCAAGATGCG 
AGTCAAATCA ACAGAAATAA CAACTTTCAG ACCTTTCCTC AGGCTGTGCT TTTACTGTTC 
AGGTGTGCAA CCGGGGAGGC CTGGCAAGAC ATCATGTTGG CAAGCCTGCC TGGGAAGCGG 
TGTGACAGCG AGTCCGACTT TGGTCCTGGG GAGGAATTTA CCTGTGGGAG TAACTTCGCT 
ATTGTGTATT TCATCAGTTT CTTCATGCTC TGCGCCTTCC TTATCATTAA CCTGTTTGTG 
GCTGTCATTA TGGACAACTT TGACTACCTC ACTAGGGATT GGTCAATTCT GGGCCCACAT 
CATTTAGATG AGTTTAAGAG GATCTGGTCG GAATACGATC CCGAAGCTAA GGGTCGCATC 
AAGCACCTGG ATGTTGTGAC CTTGCTTCGC AGGATCCAGC CTCCACTGGG ATTTGGGAAG 
CTGTGCCCCC ATCGTGTAGC ATGTAAGAGG CTGGTGGCCA TGAATATGCC TTTAAATGCA 
GATGGGACTG TCACCTTTAA TGCTACACTT TTTGCCCTTG TTCGAACATC ACTCAAGATT 
AAAACTGAAG GTAACCTAGA GACTGCCAAT GAGGAACTGA GAGCTGTCAT CAAAAAGATC 
TGGAAAAGAA CCAAGCAGAA GATACTAGAT GAGGTGATTC CCCCTCCTGA AGAGGAGGAG 
GTGACGGTTG GGAAATTTTA TGCCACATTC CTGATCCAGG ATTACTTTCG CAAGTTTCGC 
CGAAGAAAAG AAAAGGGGCT CCTGGGAACA GAGAATTTGC CTTGTAATTC CACAACACTG 
CAGGCAGGGC TGAGGAGCCT ACAGGATCTT GGTTCAGAGA TACGACGAGT GATATCCACT 
GAGCTAGAGG AGGAAGAAGA TATCTCTGGA TTCAGAGCAC TGGGGCTTCA GGTTTCAGAA 
GACAAATCAT TGTGCGAAAA CTTAACTTTG GTGCAGGCAA CTGAAAATAA GATTTCTGGA 
TCTGTAGTAT CTCTTACTAA CTCCATTGGA GGAGGAAGAG AAGAGCAGCT ACCAATGTCT 
CGAAGATCAT CACTAATAAA TCGGTCTGTA GGAGGATCTA AGGACAGTTT GTTGGTCCCA 
CCCACACCAC CACCCAATGT GTCGTCCAGC ATGAAGAGGA GGCAGATGAC CACACGGAGG 
AGAAGCTCAG AAGGCAGCGG TATCACTCCC ACTGTGCATG AAGAGGAAGA GGAGAGTGCA 
GCAGAACATG AGCTGAGATC TAGACCTCCC TCACCGGGGT ACGACCAAAG CAATGAGATA 
CAGCGCCAGT CTAGTTTGGA GGAGTCTCAG CAGCGCTCAG CATCTCCTCA GGAAAGAGTG 
AAAGTTCTGC CTCCTCCGGC TGCCCCTCCA TCTAGTTTCA GAGCGAATGG CACCTTGGAA 
GGAAGAGCAA CCCAAAGAAG GCGCCTGCTG CCTCCTACCC CAGGTACCAA GTCTGCATTT 
AGCATCCAGT GCCTGAAGAG ACAGGGGAGC TGTGATGATA TACCCATCCC AGGAACATAC 
CATCAGAATG CACCCCCATG CAGAACAAGA GGCCAGGTTT ATGGAAGTAC CGATTCTTGG 
CAAAGAAATA GTTACTCCAC CAATGGCACT CACTCATGGG CCAACCCACC AAAGCGTGGG 
CGCCTTCTTT ATGCACCCCT TATATTGGTG GAGGAAGACA GCGCCCTGCC TACTATTCCA 
TGCACTCGCT GGTATCAGGA AGAGGATAGC AAAGCACCCT ATTGCCACTA CACCCAGCTG 
CGTGTGCCGG GCCAGAGGCT TGCCGAGAAG AGAGGCAGCG CTGACAGTCT AGTGGAGGCG 
GTTTTGATAT CAGAAGGCCT AGGGCTTTAT GCACGGGACC CCAAGTTTGT CGCTTTCGCC 
AAACGTGAGA TCGCAGATGC CTGTCAGATG ACCATTGATG AGATGGAGAG TGCCGCCAGT 
GACTTGCTAA GCCAGAGCAG GAAAGGCAGT TGGACTGGAA ATAATGGACA CTCCTACCAA 
GATGAAACGG CATGGAATGG ACTTTTGTAC AGTGATGAAG ACTCTATACA CTGCCGAGAA 
GAAGAGGAAT TAAGAGATGA GATGGCCTGC GTGTCTTCAT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1f ( XM_031891456.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747316.1 Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X2, mRNA.

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CloneID	OXa64766
Clone ID Related Accession (Same CDS sequence)	XM_031891456.1
Accession Version	XM_031891456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT 
GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG 
AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC 
AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA 
GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG 
GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA 
GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC 
GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC 
ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT 
GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT 
GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC 
ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC 
ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC 
ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA 
AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA 
ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG 
GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG 
GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT 
GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA 
AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC 
CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCC TAGCCTTCCA 
GCAAGTGAGA CCACTTCTGT GAACACCGAG AATATAGACG AGGAGCATGC TAACTGCTGT 
GCCCGCTGTT TGAGCAAGCT GTCACAGACT GGGTGCTGCC GTAAGATAAG GCGGTGGAAC 
CGAGCCTTTC GACGGAAATG CCGCCTGGCT GTTAAATCGG TCACCTTCTA CTGGATGGTT 
CTGCTCCTCG TCTTCCTCAA CACGCTGACC ATTGCATCAG AGCATTACCA ACAGCCAGAT 
TGGCTGACTG AGATTCAAGC GTATGCCAAC AAGGTGCTTC TGTCTCTATT CACACTGGAG 
ATGCTTCTCA AGATGTACAG CCTTGGGTTA CACGCCTACT TTGTGTCCTT CTTTAACCGC 
TTTGATTGCT TCGTGGTTTG TGGTGGGATC TTGGAGACTG TCCTTGTAGA ATTTGACATC 
ATGTCTCCTC TGGGAATCTC TGTTCTTCGT TGTGTACGAC TATTGAGGAT CTTTAAGGTC 
ACCAGACACT GGGCATCACT AAGTAATTTG GTGGCTTCCC TTTTGAACTC CATGAAATCC 
ATTGCTTCTT TGCTGCTGCT TCTCTTCCTC TTCATTATCA TCTTCTCCTT GCTCGGAATG 
CAGCTCTTTG GGGGCAAATT CAACTTTGAT GAGACCCAGA CCAAGAGGAG CACATTTGAC 
ACCTTCCCTC AGGCCCTGCT TACTGTCTTC CAGATACTCA CAGGTGAGGA CTGGAATGCA 
GTCATGTACG ATGGGATAAT GGCGTATGGG GGACCATTTT TCCCTGGAAT GCTGGTCTGT 
GTGTATTTTA TCATCCTCTT CATATGTGGT AACTATATCC TACTCAATGT CTTTTTGGCC 
ATCGCTGTGG ACAATCTGGC TGATGGTGAC AACATCAATA GCAAAGATAA AAAGGTGAAC 
AACAAATCAG AGAAGAAGGA TGAAAACTCT GTCAAGGTTC CGTGTGAGGA GGAGAATGAA 
AACCAGGAGG AGGAAGGGAG TGAAGCCTCT GAAGAAGAGG AAGATGATGA GGGCAGCCTC 
CCAGAATCAC ACTTGGATAA AATTGCTGAC TTTGCTCCAA AGGAGAAGGT TCTTCCCATT 
CCCGAGGGCA GTGCATTCTT CATCCTCAGT AACACCAACC TCCTCCGCGT TGGGTGCCAC 
AAGCTGATTC ACCACCACAT TTTCACCAAC CTCATTTTAG TCTTCATCAT CCTCAGCAGC 
ATTTCCCTGG CAGCCGAAGA TCCAATCCGC GCCCACTCTT TTCGTAATCA TATCCTGGGG 
TATTTTGATT ATGCATTCAC GTCTATCTTC ACAGTGGAGA TTTTGCTGAA GATGACAGCA 
TATGGTGCAT TCTTGCACAA GGGCTCCTTC TGCAGGAACT GGTTCAATCT GCTGGATCTC 
CTTGTTGTCA GCGTGTCTCT CATCAGTTTT GGAATCCATT CTAGCGCTAT CTCCGTTGTA 
AAGATTCTAC GTGTTCTGAG AGTACTGCGT CCATTAAGAG CCATCAACAG AGCCAAGGGG 
CTTAAGCACG TGGTCCAGTG CGTCTTTGTT GCAATCCGCA CTATCGGTAA CATTATGATA 
GTGACTACTT TGCTGCAGTT CATGTTCGCC TGCATAGGTG TTCAGCTTTT CAAGGGCAAG 
TTTTACAGTT GCACTGATGA GGCCAAACGC AGCCCAGAGG AGTGCAAGGG CACATTCATT 
ATTTATAAGG AAGGGGACGT TTCACACCCC ATGGTGCGGG AAAGAATTTG GCACAACAGT 
GACTTCAACT TTGACAACGT CCTGTCAGGC ATGATGGCTC TGTTTACAGT GTCCACATTT 
GAAGGTTGGC CAGCGTTACT ATACAAAGCC ATTGATGCCT ATGGGGAGAA CGTAGGACCT 
ATTTACAACT ACCGTGTGGA GATCTCCATC TTTTTTATTG TCTACATTAT CATCATTGCC 
TTCTTCATGA TGAACATCTT TGTGGGTTTT GTGATTATCA CTTTCCGAGC ACAAGGAGAG 
CAGGAGTACA AGAACTGTGA ACTGGACAAG AACCAGCGCC AGTGTGTGGA ATATGCATTG 
AAGGCTCAGC CTCTGCGGCT GTACATCCCC AAGGCAAAGT ATCAGTACAA AGTCTGGTAC 
ATGGTCAACT CAACTGGCTT TGAGTACATC ATGTTTGTCC TTATCCTGCT CAACACTGTT 
GCTTTAGCCA TGCAGCATTA TGAGCAGTCC AAGCCCTTTA ATTATGCAAT GGATATCCTG 
AATATGGTCT TCACTGGACT CTTCACCGTG GAAATGGTGC TAAAGCTGAT GGCATTTCGA 
CCCAAGCATT ATTTCTGCGA TGCCTGGAAC ACATTTGATG CTCTCATTGT AGTGGGCAGC 
CTTGTGGATA TTGCAGTCAC CGAGGTCAAT AATGGATGGC ATGTTGGTGA GAGTTCAGAG 
GATAGCTCTC GTATTTCCAT CACATTCTTC CGGCTCTTCC GCGTCATGCG TTTGGTAAAG 
CTTCTTAGTA AAGGAGAGGG AATCAGGACA CTGCTGTGGA CATTCATCAA ATCCTTCCAG 
GCTTTGCCAT ACGTTGCTCT GCTCATCGCT ATGATATTTT TTATCTACGC TGTCATCGGC 
ATGCAGACAT TTGGGAAAAT TGCAATGCAA GATGCGAGTC AAATCAACAG AAATAACAAC 
TTTCAGACCT TTCCTCAGGC TGTGCTTTTA CTGTTCAGGT GTGCAACCGG GGAGGCCTGG 
CAAGACATCA TGTTGGCAAG CCTGCCTGGG AAGCGGTGTG ACAGCGAGTC CGACTTTGGT 
CCTGGGGAGG AATTTACCTG TGGGAGTAAC TTCGCTATTG TGTATTTCAT CAGTTTCTTC 
ATGCTCTGCG CCTTCCTTAT CATTAACCTG TTTGTGGCTG TCATTATGGA CAACTTTGAC 
TACCTCACTA GGGATTGGTC AATTCTGGGC CCACATCATT TAGATGAGTT TAAGAGGATC 
TGGTCGGAAT ACGATCCCGA AGCTAAGGGT CGCATCAAGC ACCTGGATGT TGTGACCTTG 
CTTCGCAGGA TCCAGCCTCC ACTGGGATTT GGGAAGCTGT GCCCCCATCG TGTAGCATGT 
AAGAGGCTGG TGGCCATGAA TATGCCTTTA AATGCAGATG GGACTGTCAC CTTTAATGCT 
ACACTTTTTG CCCTTGTTCG AACATCACTC AAGATTAAAA CTGAAGGTAA CCTAGAGACT 
GCCAATGAGG AACTGAGAGC TGTCATCAAA AAGATCTGGA AAAGAACCAA GCAGAAGATA 
CTAGATGAGG TGATTCCCCC TCCTGAAGAG GAGGAGGTGA CGGTTGGGAA ATTTTATGCC 
ACATTCCTGA TCCAGGATTA CTTTCGCAAG TTTCGCCGAA GAAAAGAAAA GGGGCTCCTG 
GGAACAGAGA ATTTGCCTTG TAATTCCACA ACACTGCAGG CAGGGCTGAG GAGCCTACAG 
GATCTTGGTT CAGAGATACG ACGAGTGATA TCCACTGAGC TAGAGGAGGA AGAAGATATC 
TCTGGATTCA GAGCACTGGG GCTTCAGGTT TCAGAAGACA AATCATTGTG CGAAAACTTA 
ACTTTGGTGC AGGCAACTGA AAATAAGATT TCTGGATCTG TAGTATCTCT TACTAACTCC 
ATTGGAGGAG GAAGAGAAGA GCAGCTACCA ATGTCTCGAA GATCATCACT AATAAATCGG 
TCTGTAGGAG GATCTAAGGA CAGTTTGTTG GTCCCACCCA CACCACCACC CAATGTGTCG 
TCCAGCATGA AGAGGAGGCA GATGACCACA CGGAGGAGAA GCTCAGAAGG CAGCGGTATC 
ACTCCCACTG TGCATGAAGA GGAAGAGGAG AGTGCAGCAG AACATGAGCT GAGATCTAGA 
CCTCCCTCAC CGGGGTACGA CCAAAGCAAT GAGATACAGC GCCAGTCTAG TTTGGAGGAG 
TCTCAGCAGC GCTCAGCATC TCCTCAGGAA AGAGTGAAAG TTCTGCCTCC TCCGGCTGCC 
CCTCCATCTA GTTTCAGAGC GAATGGCACC TTGGAAGGAA GAGCAACCCA AAGAAGGCGC 
CTGCTGCCTC CTACCCCAGG TACCAAGTCT GCATTTAGCA TCCAGTGCCT GAAGAGACAG 
GGGAGCTGTG ATGATATACC CATCCCAGGA ACATACCATC AGAATGCACC CCCATGCAGA 
ACAAGAGGCC AGGTTTATGG AAGTACCGAT TCTTGGCAAA GAAATAGTTA CTCCACCAAT 
GGCACTCACT CATGGGCCAA CCCACCAAAG CGTGGGCGCC TTCTTTATGC ACCCCTTATA 
TTGGTGGAGG AAGACAGCGC CCTGCCTACT ATTCCATGCA CTCGCTGGTA TCAGGAAGAG 
GATAGCAAAG CACCCTATTG CCACTACACC CAGCTGCGTG TGCCGGGCCA GAGGCTTGCC 
GAGAAGAGAG GCAGCGCTGA CAGTCTAGTG GAGGCGGTTT TGATATCAGA AGGCCTAGGG 
CTTTATGCAC GGGACCCCAA GTTTGTCGCT TTCGCCAAAC GTGAGATCGC AGATGCCTGT 
CAGATGACCA TTGATGAGAT GGAGAGTGCC GCCAGTGACT TGCTAAGCCA GAGCAGGAAA 
GGCAGTTGGA CTGGAAATAA TGGACACTCC TACCAAGATG AAACGGCATG GAATGGACTT 
TTGTACAGTG ATGAAGACTC TATACACTGC CGAGAAGAAG AGGAATTAAG AGATGAGATG 
GCCTGCGTGT CTTCATTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747316.1
CDS	348..6368
Translation	MSEPEENDKGPAPPYSSEEAEPFMASAEAALACWRYSMDGGPMMNGTDTLNSTASSQAQKKRQYHSKHKNQGTGGVHRSPRALFCLYLSNPIRRACISIVEWKPFDILILMTIFANCVALGVYIPFPEDDSNIANHNLEQVEYIFLIIFTVESFLKIIAYGLVLHPSAYIRNGWNLLDFVIVVIGLFSVILEQFSHKQGDAHHMSGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKAMVPLLHIALLVLFVIIIYAIIGLELFIGRMHKTCFYIGSDLVSEEEPAPCAFSGHGRECLLNNTECRGKWEGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLKGYLDWITQAEDLEPDQEIRDERHPSLPASETTSVNTENIDEEHANCCARCLSKLSQTGCCRKIRRWNRAFRRKCRLAVKSVTFYWMVLLLVFLNTLTIASEHYQQPDWLTEIQAYANKVLLSLFTLEMLLKMYSLGLHAYFVSFFNRFDCFVVCGGILETVLVEFDIMSPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDTFPQALLTVFQILTGEDWNAVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLADGDNINSKDKKVNNKSEKKDENSVKVPCEEENENQEEEGSEASEEEEDDEGSLPESHLDKIADFAPKEKVLPIPEGSAFFILSNTNLLRVGCHKLIHHHIFTNLILVFIILSSISLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTAYGAFLHKGSFCRNWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKRSPEECKGTFIIYKEGDVSHPMVRERIWHNSDFNFDNVLSGMMALFTVSTFEGWPALLYKAIDAYGENVGPIYNYRVEISIFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYKNCELDKNQRQCVEYALKAQPLRLYIPKAKYQYKVWYMVNSTGFEYIMFVLILLNTVALAMQHYEQSKPFNYAMDILNMVFTGLFTVEMVLKLMAFRPKHYFCDAWNTFDALIVVGSLVDIAVTEVNNGWHVGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQTFGKIAMQDASQINRNNNFQTFPQAVLLLFRCATGEAWQDIMLASLPGKRCDSESDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNADGTVTFNATLFALVRTSLKIKTEGNLETANEELRAVIKKIWKRTKQKILDEVIPPPEEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGTENLPCNSTTLQAGLRSLQDLGSEIRRVISTELEEEEDISGFRALGLQVSEDKSLCENLTLVQATENKISGSVVSLTNSIGGGREEQLPMSRRSSLINRSVGGSKDSLLVPPTPPPNVSSSMKRRQMTTRRRSSEGSGITPTVHEEEEESAAEHELRSRPPSPGYDQSNEIQRQSSLEESQQRSASPQERVKVLPPPAAPPSSFRANGTLEGRATQRRRLLPPTPGTKSAFSIQCLKRQGSCDDIPIPGTYHQNAPPCRTRGQVYGSTDSWQRNSYSTNGTHSWANPPKRGRLLYAPLILVEEDSALPTIPCTRWYQEEDSKAPYCHYTQLRVPGQRLAEKRGSADSLVEAVLISEGLGLYARDPKFVAFAKREIADACQMTIDEMESAASDLLSQSRKGSWTGNNGHSYQDETAWNGLLYSDEDSIHCREEEELRDEMACVSSF

Target ORF information:

RefSeq Version	XM_031891456.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, L type, alpha 1F subunit (cacna1f), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891456.1

ORF Insert Sequence:

ATGTCAGAAC CAGAGGAGAA TGACAAAGGC CCTGCTCCTC CATACTCCAG CGAGGAGGCT 
GAGCCGTTCA TGGCAAGTGC AGAGGCAGCG CTGGCCTGTT GGCGATACTC CATGGATGGT 
GGGCCAATGA TGAATGGCAC CGACACCTTA AACTCCACTG CCAGCTCCCA GGCGCAGAAG 
AAACGGCAGT ACCACAGCAA GCACAAGAAC CAGGGAACAG GGGGAGTGCA TAGGAGCCCC 
AGGGCTCTCT TTTGTCTGTA CCTCAGCAAC CCCATTCGAA GGGCCTGCAT CAGCATTGTA 
GAATGGAAAC CATTTGATAT TCTCATTCTC ATGACTATTT TCGCCAACTG CGTGGCGTTG 
GGTGTCTACA TTCCGTTTCC CGAGGACGAT TCCAACATTG CCAATCACAA CCTGGAACAA 
GTGGAGTATA TTTTCCTCAT TATCTTCACC GTGGAGTCCT TCCTAAAGAT AATTGCATAC 
GGGCTGGTCT TGCACCCTAG TGCCTACATC AGAAACGGCT GGAACCTGCT GGACTTTGTC 
ATTGTTGTTA TTGGGTTATT CAGTGTTATT TTGGAACAGT TCTCTCACAA GCAAGGAGAT 
GCCCACCACA TGAGTGGAAA GCCAGGAGGG TTTGATGTGA AAGCTCTGCG TGCATTTCGT 
GTGTTAAGAC CTCTCAGGCT GGTATCTGGA GTCCCAAGCT TGCACATTGT ATTAAATTCC 
ATAATGAAAG CCATGGTTCC CTTATTGCAC ATTGCCTTGC TTGTCCTCTT TGTTATCATC 
ATTTATGCCA TTATTGGGCT GGAGCTCTTC ATTGGTCGGA TGCACAAGAC ATGTTTCTAC 
ATTGGTTCAG ATCTAGTTTC AGAGGAGGAA CCAGCCCCCT GTGCTTTCTC AGGACACGGA 
AGAGAATGTC TCCTCAACAA TACAGAGTGC AGAGGAAAGT GGGAAGGGCC TAACGGGGGA 
ATCACAAACT TTGATAACTT CTTCTTTGCC ATGCTGACTG TGTTCCAATG TATCACTATG 
GAAGGTTGGA CAGATGTTCT CTACTGGATG CAGGATGCCA TGGGTTATGA GCTTCCCTGG 
GTATATTTCG TCAGCCTTGT TATTTTTGGA TCATTCTTTG TTTTGAACCT TGTATTGGGT 
GTGTTGAGTG GAGAATTTTC TAAAGAAAGA GAAAAGGCCA AGGCCCGAGG GGACTTCCAA 
AAGCTGAGGG AGAAACAGCA GATGGAAGAG GATCTCAAGG GATACCTAGA CTGGATCACC 
CAGGCCGAAG ATCTTGAACC TGATCAAGAA ATCAGAGATG AGCGGCACCC TAGCCTTCCA 
GCAAGTGAGA CCACTTCTGT GAACACCGAG AATATAGACG AGGAGCATGC TAACTGCTGT 
GCCCGCTGTT TGAGCAAGCT GTCACAGACT GGGTGCTGCC GTAAGATAAG GCGGTGGAAC 
CGAGCCTTTC GACGGAAATG CCGCCTGGCT GTTAAATCGG TCACCTTCTA CTGGATGGTT 
CTGCTCCTCG TCTTCCTCAA CACGCTGACC ATTGCATCAG AGCATTACCA ACAGCCAGAT 
TGGCTGACTG AGATTCAAGC GTATGCCAAC AAGGTGCTTC TGTCTCTATT CACACTGGAG 
ATGCTTCTCA AGATGTACAG CCTTGGGTTA CACGCCTACT TTGTGTCCTT CTTTAACCGC 
TTTGATTGCT TCGTGGTTTG TGGTGGGATC TTGGAGACTG TCCTTGTAGA ATTTGACATC 
ATGTCTCCTC TGGGAATCTC TGTTCTTCGT TGTGTACGAC TATTGAGGAT CTTTAAGGTC 
ACCAGACACT GGGCATCACT AAGTAATTTG GTGGCTTCCC TTTTGAACTC CATGAAATCC 
ATTGCTTCTT TGCTGCTGCT TCTCTTCCTC TTCATTATCA TCTTCTCCTT GCTCGGAATG 
CAGCTCTTTG GGGGCAAATT CAACTTTGAT GAGACCCAGA CCAAGAGGAG CACATTTGAC 
ACCTTCCCTC AGGCCCTGCT TACTGTCTTC CAGATACTCA CAGGTGAGGA CTGGAATGCA 
GTCATGTACG ATGGGATAAT GGCGTATGGG GGACCATTTT TCCCTGGAAT GCTGGTCTGT 
GTGTATTTTA TCATCCTCTT CATATGTGGT AACTATATCC TACTCAATGT CTTTTTGGCC 
ATCGCTGTGG ACAATCTGGC TGATGGTGAC AACATCAATA GCAAAGATAA AAAGGTGAAC 
AACAAATCAG AGAAGAAGGA TGAAAACTCT GTCAAGGTTC CGTGTGAGGA GGAGAATGAA 
AACCAGGAGG AGGAAGGGAG TGAAGCCTCT GAAGAAGAGG AAGATGATGA GGGCAGCCTC 
CCAGAATCAC ACTTGGATAA AATTGCTGAC TTTGCTCCAA AGGAGAAGGT TCTTCCCATT 
CCCGAGGGCA GTGCATTCTT CATCCTCAGT AACACCAACC TCCTCCGCGT TGGGTGCCAC 
AAGCTGATTC ACCACCACAT TTTCACCAAC CTCATTTTAG TCTTCATCAT CCTCAGCAGC 
ATTTCCCTGG CAGCCGAAGA TCCAATCCGC GCCCACTCTT TTCGTAATCA TATCCTGGGG 
TATTTTGATT ATGCATTCAC GTCTATCTTC ACAGTGGAGA TTTTGCTGAA GATGACAGCA 
TATGGTGCAT TCTTGCACAA GGGCTCCTTC TGCAGGAACT GGTTCAATCT GCTGGATCTC 
CTTGTTGTCA GCGTGTCTCT CATCAGTTTT GGAATCCATT CTAGCGCTAT CTCCGTTGTA 
AAGATTCTAC GTGTTCTGAG AGTACTGCGT CCATTAAGAG CCATCAACAG AGCCAAGGGG 
CTTAAGCACG TGGTCCAGTG CGTCTTTGTT GCAATCCGCA CTATCGGTAA CATTATGATA 
GTGACTACTT TGCTGCAGTT CATGTTCGCC TGCATAGGTG TTCAGCTTTT CAAGGGCAAG 
TTTTACAGTT GCACTGATGA GGCCAAACGC AGCCCAGAGG AGTGCAAGGG CACATTCATT 
ATTTATAAGG AAGGGGACGT TTCACACCCC ATGGTGCGGG AAAGAATTTG GCACAACAGT 
GACTTCAACT TTGACAACGT CCTGTCAGGC ATGATGGCTC TGTTTACAGT GTCCACATTT 
GAAGGTTGGC CAGCGTTACT ATACAAAGCC ATTGATGCCT ATGGGGAGAA CGTAGGACCT 
ATTTACAACT ACCGTGTGGA GATCTCCATC TTTTTTATTG TCTACATTAT CATCATTGCC 
TTCTTCATGA TGAACATCTT TGTGGGTTTT GTGATTATCA CTTTCCGAGC ACAAGGAGAG 
CAGGAGTACA AGAACTGTGA ACTGGACAAG AACCAGCGCC AGTGTGTGGA ATATGCATTG 
AAGGCTCAGC CTCTGCGGCT GTACATCCCC AAGGCAAAGT ATCAGTACAA AGTCTGGTAC 
ATGGTCAACT CAACTGGCTT TGAGTACATC ATGTTTGTCC TTATCCTGCT CAACACTGTT 
GCTTTAGCCA TGCAGCATTA TGAGCAGTCC AAGCCCTTTA ATTATGCAAT GGATATCCTG 
AATATGGTCT TCACTGGACT CTTCACCGTG GAAATGGTGC TAAAGCTGAT GGCATTTCGA 
CCCAAGCATT ATTTCTGCGA TGCCTGGAAC ACATTTGATG CTCTCATTGT AGTGGGCAGC 
CTTGTGGATA TTGCAGTCAC CGAGGTCAAT AATGGATGGC ATGTTGGTGA GAGTTCAGAG 
GATAGCTCTC GTATTTCCAT CACATTCTTC CGGCTCTTCC GCGTCATGCG TTTGGTAAAG 
CTTCTTAGTA AAGGAGAGGG AATCAGGACA CTGCTGTGGA CATTCATCAA ATCCTTCCAG 
GCTTTGCCAT ACGTTGCTCT GCTCATCGCT ATGATATTTT TTATCTACGC TGTCATCGGC 
ATGCAGACAT TTGGGAAAAT TGCAATGCAA GATGCGAGTC AAATCAACAG AAATAACAAC 
TTTCAGACCT TTCCTCAGGC TGTGCTTTTA CTGTTCAGGT GTGCAACCGG GGAGGCCTGG 
CAAGACATCA TGTTGGCAAG CCTGCCTGGG AAGCGGTGTG ACAGCGAGTC CGACTTTGGT 
CCTGGGGAGG AATTTACCTG TGGGAGTAAC TTCGCTATTG TGTATTTCAT CAGTTTCTTC 
ATGCTCTGCG CCTTCCTTAT CATTAACCTG TTTGTGGCTG TCATTATGGA CAACTTTGAC 
TACCTCACTA GGGATTGGTC AATTCTGGGC CCACATCATT TAGATGAGTT TAAGAGGATC 
TGGTCGGAAT ACGATCCCGA AGCTAAGGGT CGCATCAAGC ACCTGGATGT TGTGACCTTG 
CTTCGCAGGA TCCAGCCTCC ACTGGGATTT GGGAAGCTGT GCCCCCATCG TGTAGCATGT 
AAGAGGCTGG TGGCCATGAA TATGCCTTTA AATGCAGATG GGACTGTCAC CTTTAATGCT 
ACACTTTTTG CCCTTGTTCG AACATCACTC AAGATTAAAA CTGAAGGTAA CCTAGAGACT 
GCCAATGAGG AACTGAGAGC TGTCATCAAA AAGATCTGGA AAAGAACCAA GCAGAAGATA 
CTAGATGAGG TGATTCCCCC TCCTGAAGAG GAGGAGGTGA CGGTTGGGAA ATTTTATGCC 
ACATTCCTGA TCCAGGATTA CTTTCGCAAG TTTCGCCGAA GAAAAGAAAA GGGGCTCCTG 
GGAACAGAGA ATTTGCCTTG TAATTCCACA ACACTGCAGG CAGGGCTGAG GAGCCTACAG 
GATCTTGGTT CAGAGATACG ACGAGTGATA TCCACTGAGC TAGAGGAGGA AGAAGATATC 
TCTGGATTCA GAGCACTGGG GCTTCAGGTT TCAGAAGACA AATCATTGTG CGAAAACTTA 
ACTTTGGTGC AGGCAACTGA AAATAAGATT TCTGGATCTG TAGTATCTCT TACTAACTCC 
ATTGGAGGAG GAAGAGAAGA GCAGCTACCA ATGTCTCGAA GATCATCACT AATAAATCGG 
TCTGTAGGAG GATCTAAGGA CAGTTTGTTG GTCCCACCCA CACCACCACC CAATGTGTCG 
TCCAGCATGA AGAGGAGGCA GATGACCACA CGGAGGAGAA GCTCAGAAGG CAGCGGTATC 
ACTCCCACTG TGCATGAAGA GGAAGAGGAG AGTGCAGCAG AACATGAGCT GAGATCTAGA 
CCTCCCTCAC CGGGGTACGA CCAAAGCAAT GAGATACAGC GCCAGTCTAG TTTGGAGGAG 
TCTCAGCAGC GCTCAGCATC TCCTCAGGAA AGAGTGAAAG TTCTGCCTCC TCCGGCTGCC 
CCTCCATCTA GTTTCAGAGC GAATGGCACC TTGGAAGGAA GAGCAACCCA AAGAAGGCGC 
CTGCTGCCTC CTACCCCAGG TACCAAGTCT GCATTTAGCA TCCAGTGCCT GAAGAGACAG 
GGGAGCTGTG ATGATATACC CATCCCAGGA ACATACCATC AGAATGCACC CCCATGCAGA 
ACAAGAGGCC AGGTTTATGG AAGTACCGAT TCTTGGCAAA GAAATAGTTA CTCCACCAAT 
GGCACTCACT CATGGGCCAA CCCACCAAAG CGTGGGCGCC TTCTTTATGC ACCCCTTATA 
TTGGTGGAGG AAGACAGCGC CCTGCCTACT ATTCCATGCA CTCGCTGGTA TCAGGAAGAG 
GATAGCAAAG CACCCTATTG CCACTACACC CAGCTGCGTG TGCCGGGCCA GAGGCTTGCC 
GAGAAGAGAG GCAGCGCTGA CAGTCTAGTG GAGGCGGTTT TGATATCAGA AGGCCTAGGG 
CTTTATGCAC GGGACCCCAA GTTTGTCGCT TTCGCCAAAC GTGAGATCGC AGATGCCTGT 
CAGATGACCA TTGATGAGAT GGAGAGTGCC GCCAGTGACT TGCTAAGCCA GAGCAGGAAA 
GGCAGTTGGA CTGGAAATAA TGGACACTCC TACCAAGATG AAACGGCATG GAATGGACTT 
TTGTACAGTG ATGAAGACTC TATACACTGC CGAGAAGAAG AGGAATTAAG AGATGAGATG 
GCCTGCGTGT CTTCATTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cacna1f cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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