mio cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mio
Entrez Gene ID	33399
Full Name	missing oocyte
Synonyms	CG15384,CG7074,Dmel\CG7074,FBtr0077835,Mio,SEA4,Sea4
General protein information	Preferred Names missing oocyte Names CG7074 gene product from transcript CG7074-RA CG7074-PA mio-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 22D4-22D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_134812.3	NP_608656.1	missing oocyte

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MIOS	XP_001144783.1
Drosophila melanogaster (fruit fly)	mio	NP_608656.1
Xenopus tropicalis (tropical clawed frog)	mios	XP_004915476.1
	MIOS	XP_001087456.1
Canis lupus familiaris (dog)	MIOS	XP_005628601.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007984	XP_317482.4
Homo sapiens (human)	MIOS	NP_061878.3
Bos taurus (cattle)	MIOS	NP_001179247.1
Gallus gallus (chicken)	MIOS	XP_418678.2
Rattus norvegicus (Norway rat)	Mios	NP_001167025.1
Mus musculus (house mouse)	Mios	NP_663349.2
Danio rerio (zebrafish)	mios	NP_958490.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Like mio, seh1 has a crucial germline function during oogenesis. In both mio and seh1 mutant ovaries, a fraction of oocytes fail to maintain the meiotic cycle and develop as pseudo-nurse cells.
Title: The nucleoporin Seh1 forms a complex with Mio and serves an essential tissue-specific function in Drosophila oogenesis.

product of the missing oocyte gene acts in the oocyte nucleus to facilitate the execution of the unique cell cycle and developmental programs that produce the mature haploid gamete
Title: missing oocyte encodes a highly conserved nuclear protein required for the maintenance of the meiotic cycle and oocyte identity in Drosophila.

The following mio gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mio cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16115	NM_134812.3 Latest version!	Drosophila melanogaster missing oocyte (mio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mio ( NM_134812.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608656.1 Drosophila melanogaster missing oocyte (mio), mRNA.

Price & Availability↑

CloneID	OFa16115
Clone ID Related Accession (Same CDS sequence)	NM_134812.3
Accession Version	NM_134812.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	missing oocyte
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134812.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGCA ATACACACGG ACTCAGCTGG TTTCCACATT TTCCCGACAA ATTCGTATCC 
TGGGGCCAGG AAATCCATTT ATACGAAGTG CGCCGCAAAG ATGATCACAG CCAAAAAAGT 
CGCCTGCCTT ATATATCCGT GAACTACCTC GCCAACGAGT CGCGCTATCA ATATGCCCGC 
TGCGTGGCCG CCTCCTACCA CAGTGACCAG CCCATAATTG CAGTGGGTCT GGCGGATGGC 
AAAGTCGGGA TTTGCAACTT CCGGGACACC TACGACAGCA GCTGGGAGTA CACACCGCGT 
CAGCAGCGCA TGTGCACCTG CCTCGCCTGG AACGAGCTGG ATGCCAATAT CCTGGCCATT 
GGACACGATC GCCATCGAAA TGACACCTGC ATCACCATCT GGGACATTGA GCGTGGAGTT 
CCCAAGGAGA CGGCCAACTT CTTCGGCGTC GGCGAGTCGG CCAACTCCAT TTGCTGGGAT 
CGAAATCATC GCACGGTCAT TGCGGGAATG AGCCAGAAGA TGATAAAGCT GTTCGATCTG 
AGGCAGAGTA ACGCCACCTG TCAAAGCATT CAAACAAAGA CAGTGCAGGG ACTTTCCGTA 
TCCCCCAATG GCAACTATCT CTGCAGCTAT GTGGACTCGG TGATCACTCT GTGGGATCCG 
CGAAATATCA AGAGTCCCCT CAGGCAGATT CAGTCCTCGA AAAACCATTT GCAGATCGCT 
TGGTGTCCGA CCAGGACAAG CCTGCTTTCC TCGCTGCAAC GCGATTCCTC CTACATCACT 
CTCTACGACA TCCGGAGTGT GGACACGGAC AATTCCGGAG AGATCTACCA CGTAAAACGA 
CAGATTAGCC CCTTTCCGGC TAGATACCAG CACAGTGGAA AGTTTTCGTT TGTTAATTGC 
CTGTCGTGGC ATTCGAGGGA TTTCGAGAGA GCTCTGCTTT TGGCAGATGC GCTGAACATC 
CTGGACTTTC GACTGCCAGC CACCTTGCAC ACGGCGCACA GCAATCGCCG GAAATTGCCA 
CTTCTGATGC AGCGTCCGCT CTACACACCC GCCTCGCCGA CTTCCACTGC GGCCACGCCC 
ACTCAGCAGC AGCCCACGAG CAGCTGCAGC ACGAATAGCG GGAGTTCCCT GGACTTTAGC 
ACTCCTGGTG GTTCGCCCTT CAATGTGGAC CTGCTGAAAC CGGAACTCTT TGAGCTGGAT 
CTGGTGGACG AGACGCGTCA GCGCGCTCTA GAGGATTACG GCATCAAACC CGATAACAAG 
CGATTCGGCG AACTCCACTT GACACCGTAT CTGCGAAATG TGTGGTCCAC TTTGAACAAT 
GTCTACAGCG AGGATCGACT GACGGGCTTA AAGGCCACTT TGGGCATTAA CTTGGGACAC 
ACCTCGGAGG CCTTGATGGC CAGCTCCCGC ATTGAATCTC AGGTGCTCCA GTGGCCAGAG 
GGCATCAACA ACTCCAACAA ACTGATATGC TACAGGAGTG AGCAGCGCGA CCTGGCCCTC 
CAACTTTGCG GTTGGGCCTT TGAGCAGGAG CTGGATCGCT TTATAGACCA GTTGTATGCA 
AACAAGGAGT ACAGCCGGGC AGCCATGATC TGCGTGTTCC ACTTGAAAAT TTTCCATGCC 
TGCAACATCC TATCGTCGGC GGCGGACAAC ATGAGGGATC CCAGCATGTA CAGGATCACC 
GTCATCGCCT TGTCCAGTTT CAATGCAGAT CGCTGCAGTT CCACGTGGCG GAATCAGCGA 
TCCAGCGCCA ACATGCAGAT ACACGATCCA CACTTAAGGG CTGTCTTCTC CTTTCTAACG 
ATGGAGAAGG ACAATTTTGA TGCAGTGCTC AAAGAGGAAG GCGTTTCGTT ATCAGACCGC 
ATGGCCTTCG CCTGCAAATA TCTATCGGAG ACCAAGCTGG CTGACTATGT TGCGCAGCAA 
ATCCAGGCGG CCATAGATGG TGGCGATCTA AATGGCTTGT TGCTCACTGG AGAGTCGCAG 
GACGGAATCG ATATACTGCA GTCCTATATG GACACCAGCT TCGATGTGCA GACGGTAGCC 
CTGGTGGCCA TTAACTACTT CCGGCAAGAG CTTTTCGAGG ACAAGCGCAT CCAGTACTGG 
ATCGCCAGCT ACTTGGATCA CCTCAATAGC TGGGGCTTGT GGGAGAAGCG GGCCGAGCTG 
GACATCAAGA TCGAGAGCAT CCGCCCATCC TCCCGCAGTT CCCGCACCGT ATTTCTGTCG 
TGCAACTTTT GCGGCAAGTC TGTGTCCAAC GCCTTGCTGG ACGAGCCGCG TCCACGGAGC 
ACCACCACCA GCACCAACAG GCTCTCCTCA TGCCCCAGCT GCCGGAAACC CCTGCCCCGC 
TGCTCGCTCT GCCTAATGCA CATGGGCACA ATGGTGAACA TGAGCAATGG GGAGACCCCG 
ACCACCACGC CCGACGTGCC CGGCTGGCAG ACCAAGCCCT TCTCAAAGTG GTTTTCCTGG 
TGTCAGACCT GTCGCCACGG CGGACACACC GAGCACATCA TGCAGTGGTT CAAGCAAAAT 
TCAGAGTGCC CCGTGTCGTC GTGCAACTGC CGCTGCTTCG ACATGGACGG CACCAAGCCG 
AACACATTGA GAGACATTTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608656.1
CDS	138..2741
Translation	MSGNTHGLSWFPHFPDKFVSWGQEIHLYEVRRKDDHSQKSRLPYISVNYLANESRYQYARCVAASYHSDQPIIAVGLADGKVGICNFRDTYDSSWEYTPRQQRMCTCLAWNELDANILAIGHDRHRNDTCITIWDIERGVPKETANFFGVGESANSICWDRNHRTVIAGMSQKMIKLFDLRQSNATCQSIQTKTVQGLSVSPNGNYLCSYVDSVITLWDPRNIKSPLRQIQSSKNHLQIAWCPTRTSLLSSLQRDSSYITLYDIRSVDTDNSGEIYHVKRQISPFPARYQHSGKFSFVNCLSWHSRDFERALLLADALNILDFRLPATLHTAHSNRRKLPLLMQRPLYTPASPTSTAATPTQQQPTSSCSTNSGSSLDFSTPGGSPFNVDLLKPELFELDLVDETRQRALEDYGIKPDNKRFGELHLTPYLRNVWSTLNNVYSEDRLTGLKATLGINLGHTSEALMASSRIESQVLQWPEGINNSNKLICYRSEQRDLALQLCGWAFEQELDRFIDQLYANKEYSRAAMICVFHLKIFHACNILSSAADNMRDPSMYRITVIALSSFNADRCSSTWRNQRSSANMQIHDPHLRAVFSFLTMEKDNFDAVLKEEGVSLSDRMAFACKYLSETKLADYVAQQIQAAIDGGDLNGLLLTGESQDGIDILQSYMDTSFDVQTVALVAINYFRQELFEDKRIQYWIASYLDHLNSWGLWEKRAELDIKIESIRPSSRSSRTVFLSCNFCGKSVSNALLDEPRPRSTTTSTNRLSSCPSCRKPLPRCSLCLMHMGTMVNMSNGETPTTTPDVPGWQTKPFSKWFSWCQTCRHGGHTEHIMQWFKQNSECPVSSCNCRCFDMDGTKPNTLRDIS

Target ORF information:

RefSeq Version	NM_134812.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster missing oocyte (mio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134812.3

ORF Insert Sequence:

ATGAGCGGCA ATACACACGG ACTCAGCTGG TTTCCACATT TTCCCGACAA ATTCGTATCC 
TGGGGCCAGG AAATCCATTT ATACGAAGTG CGCCGCAAAG ATGATCACAG CCAAAAAAGT 
CGCCTGCCTT ATATATCCGT GAACTACCTC GCCAACGAGT CGCGCTATCA ATATGCCCGC 
TGCGTGGCCG CCTCCTACCA CAGTGACCAG CCCATAATTG CAGTGGGTCT GGCGGATGGC 
AAAGTCGGGA TTTGCAACTT CCGGGACACC TACGACAGCA GCTGGGAGTA CACACCGCGT 
CAGCAGCGCA TGTGCACCTG CCTCGCCTGG AACGAGCTGG ATGCCAATAT CCTGGCCATT 
GGACACGATC GCCATCGAAA TGACACCTGC ATCACCATCT GGGACATTGA GCGTGGAGTT 
CCCAAGGAGA CGGCCAACTT CTTCGGCGTC GGCGAGTCGG CCAACTCCAT TTGCTGGGAT 
CGAAATCATC GCACGGTCAT TGCGGGAATG AGCCAGAAGA TGATAAAGCT GTTCGATCTG 
AGGCAGAGTA ACGCCACCTG TCAAAGCATT CAAACAAAGA CAGTGCAGGG ACTTTCCGTA 
TCCCCCAATG GCAACTATCT CTGCAGCTAT GTGGACTCGG TGATCACTCT GTGGGATCCG 
CGAAATATCA AGAGTCCCCT CAGGCAGATT CAGTCCTCGA AAAACCATTT GCAGATCGCT 
TGGTGTCCGA CCAGGACAAG CCTGCTTTCC TCGCTGCAAC GCGATTCCTC CTACATCACT 
CTCTACGACA TCCGGAGTGT GGACACGGAC AATTCCGGAG AGATCTACCA CGTAAAACGA 
CAGATTAGCC CCTTTCCGGC TAGATACCAG CACAGTGGAA AGTTTTCGTT TGTTAATTGC 
CTGTCGTGGC ATTCGAGGGA TTTCGAGAGA GCTCTGCTTT TGGCAGATGC GCTGAACATC 
CTGGACTTTC GACTGCCAGC CACCTTGCAC ACGGCGCACA GCAATCGCCG GAAATTGCCA 
CTTCTGATGC AGCGTCCGCT CTACACACCC GCCTCGCCGA CTTCCACTGC GGCCACGCCC 
ACTCAGCAGC AGCCCACGAG CAGCTGCAGC ACGAATAGCG GGAGTTCCCT GGACTTTAGC 
ACTCCTGGTG GTTCGCCCTT CAATGTGGAC CTGCTGAAAC CGGAACTCTT TGAGCTGGAT 
CTGGTGGACG AGACGCGTCA GCGCGCTCTA GAGGATTACG GCATCAAACC CGATAACAAG 
CGATTCGGCG AACTCCACTT GACACCGTAT CTGCGAAATG TGTGGTCCAC TTTGAACAAT 
GTCTACAGCG AGGATCGACT GACGGGCTTA AAGGCCACTT TGGGCATTAA CTTGGGACAC 
ACCTCGGAGG CCTTGATGGC CAGCTCCCGC ATTGAATCTC AGGTGCTCCA GTGGCCAGAG 
GGCATCAACA ACTCCAACAA ACTGATATGC TACAGGAGTG AGCAGCGCGA CCTGGCCCTC 
CAACTTTGCG GTTGGGCCTT TGAGCAGGAG CTGGATCGCT TTATAGACCA GTTGTATGCA 
AACAAGGAGT ACAGCCGGGC AGCCATGATC TGCGTGTTCC ACTTGAAAAT TTTCCATGCC 
TGCAACATCC TATCGTCGGC GGCGGACAAC ATGAGGGATC CCAGCATGTA CAGGATCACC 
GTCATCGCCT TGTCCAGTTT CAATGCAGAT CGCTGCAGTT CCACGTGGCG GAATCAGCGA 
TCCAGCGCCA ACATGCAGAT ACACGATCCA CACTTAAGGG CTGTCTTCTC CTTTCTAACG 
ATGGAGAAGG ACAATTTTGA TGCAGTGCTC AAAGAGGAAG GCGTTTCGTT ATCAGACCGC 
ATGGCCTTCG CCTGCAAATA TCTATCGGAG ACCAAGCTGG CTGACTATGT TGCGCAGCAA 
ATCCAGGCGG CCATAGATGG TGGCGATCTA AATGGCTTGT TGCTCACTGG AGAGTCGCAG 
GACGGAATCG ATATACTGCA GTCCTATATG GACACCAGCT TCGATGTGCA GACGGTAGCC 
CTGGTGGCCA TTAACTACTT CCGGCAAGAG CTTTTCGAGG ACAAGCGCAT CCAGTACTGG 
ATCGCCAGCT ACTTGGATCA CCTCAATAGC TGGGGCTTGT GGGAGAAGCG GGCCGAGCTG 
GACATCAAGA TCGAGAGCAT CCGCCCATCC TCCCGCAGTT CCCGCACCGT ATTTCTGTCG 
TGCAACTTTT GCGGCAAGTC TGTGTCCAAC GCCTTGCTGG ACGAGCCGCG TCCACGGAGC 
ACCACCACCA GCACCAACAG GCTCTCCTCA TGCCCCAGCT GCCGGAAACC CCTGCCCCGC 
TGCTCGCTCT GCCTAATGCA CATGGGCACA ATGGTGAACA TGAGCAATGG GGAGACCCCG 
ACCACCACGC CCGACGTGCC CGGCTGGCAG ACCAAGCCCT TCTCAAAGTG GTTTTCCTGG 
TGTCAGACCT GTCGCCACGG CGGACACACC GAGCACATCA TGCAGTGGTT CAAGCAAAAT 
TCAGAGTGCC CCGTGTCGTC GTGCAACTGC CGCTGCTTCG ACATGGACGG CACCAAGCCG 
AACACATTGA GAGACATTTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mio cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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