FASN1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	FASN1
Entrez Gene ID	33524
Full Name	Fatty acid synthase 1
Synonyms	BcDNA:GH07626,BcDNA:gh07626,CG3523,DM_7295848,Dmel\CG3523,FAS,FASN,FASN[CG3523],FAS[CG3523],Fas,Fatty acid synthase,anon-EST:Posey235,anon-EST:Posey43,anon-WO0138581.1,dFAS,fas
General protein information	Preferred Names Fatty acid synthase 1 Names CG3523 gene product from transcript CG3523-RA CG3523-PA CG3523-PC FASN1-PA FASN1-PC fatty acid synthase fatty acid synthase 1 fatty acid synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23C5-23D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_134904.3	NP_608748.1	fatty acid synthase 1, isoform A
NM_001144306.2	NP_001137778.2	fatty acid synthase 1, isoform C

Pathways from BioSystems

Homologies

Neurospora crassa	NCU06013	XP_958135.2
Danio rerio (zebrafish)	LOC559001	XP_687387.4
Danio rerio (zebrafish)	fasn	XP_005169535.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009176	XP_319941.4
Magnaporthe oryzae (rice blast fungus)	MGG_05589	XP_003710466.1
Drosophila melanogaster (fruit fly)	CG3523	NP_608748.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic interactions at the cell-autonomous level, where glycolytic enzymes or Glo1 were manipulated in FASN mutant cells, revealed that this sugar-dependent size reduction is a direct consequence of MG-derived-AGE accumulation
Title: Fatty acid synthase cooperates with glyoxalase 1 to protect against sugar toxicity.

The following FASN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FASN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13600	NM_134904.3 Latest version!	Drosophila melanogaster fatty acid synthase 1, transcript variant A (FASN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa13601	NM_001144306.2 Latest version!	Drosophila melanogaster fatty acid synthase 1, transcript variant C (FASN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FASN1 ( NM_134904.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608748.1 Drosophila melanogaster fatty acid synthase 1, transcript variant A (FASN1), mRNA.

Price & Availability↑

CloneID	OFa13600
Clone ID Related Accession (Same CDS sequence)	NM_134904.3
Accession Version	NM_134904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fatty acid synthase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134904.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGCCC GATTCGCCGA GGAAGTCATC ACCGCTGAGC CCGCACAACG TGCCGCCCCC 
CAATTGGACC TGGGTGGTGG TCACTATGTG CCCCGTCAGC AGCACCTCAA CGATGAGATC 
GCCATCACCG GTTTCTCCGG CCGTCTGCCG GAGAGCTCCA CCATCGAGGA GTTCAAGCAG 
AATCTTTTCG ACGGCGTCGA CATGGTCAAC GATGATCCCC GCCGTTGGGA GCGTGGTCTG 
TATGGACTTC CCGACAGGAT TGGCAAGCTC AAGGATAGCG ATCTGGAGAA CTTTGACCAA 
CAGTTCTTCG GTGTGCACCA AAAGCAGGCT GAGTGCATGG ACCCACTGCT GCGCATGCTG 
CTCGAGCTCA CCCATGAAGC TATTATTGAC GCTGGCCTAA ACCCAAGTGA TCTGCGCGGT 
TCCCGCACTG GTGTGTACAT CGGTGTGTCC AACTCGGAGA CTGAGCAGCA CTGGTGCAGC 
GATGCTGACC GGGTTAACGG CTACGGCTTG ACAGGATGTG CCCGTGCCAT GTTTGCCAAC 
CGCATCTCCT TCACCTTTGA TTTCAAGGGA CCCAGCTACA GCATTGACAC CGCTTGCTCC 
AGTTCTCTGT ACGCCTTGGA ACAGGCTTTC TCCGATATGC GCGAAGGAAA GGTCGACAAC 
GCCCTGGTCG CTGGAGCTGG ACTGATCCTC AAGCCCACCA TGTCGCTGCA GTTCAAGCGA 
CTGAATATGT TGAGCCCGGA CGGCAGCTGC AAGGCCTTCG ATGAGTCTGG CAATGGATAC 
GTCCGTTCCG ATGGATGTGT GGTGCTGCTC CTGCAGCGCA CCTCTGCAGC CAGGCGTGTG 
TATGCCTCCA TCCTCAATGT GCGCACCAAT ACGGATGGTT TCAAGGAGCA GGGCATCACA 
TACCCTATTG GCAAGATGCA AAATCGCCTG ATCCGCGAGA CCTACGAGGA GATTGGTCTT 
AACCCCGCCG ATGTGGTTTA CGTGGAGGCA CACGGTACCG GAACCAAGGT GGGCGATCCC 
CAGGAGGTGA ACTCTATCAC TGACTTCTTC TGCAAGGACC GTACGACCCC TCTGTTGATC 
GGATCGGTCA AGTCCAACAT GGGTCACTCG GAACCCGCTT CCGGTGTCTG CTCTGTGGCC 
AAGATTCTGA TCGCCATGGA GGAGGGCGTC ATTCCCGGTA ACTTGCACTA CAACAAGCCG 
AACCCAGATC TTTATGGACT CGTTGATGGA CGTCTCAAGG TGGTCGACAG GAATCTGCCT 
TGGAACGGTG GCATCATCGG CCTGAACTCC TTTGGTTTTG GAGGAGCCAA CGCTCACGTT 
ATCTTAAAGT CGAACCCCAA GCCCAAGGCT TTGACCCCCA AGGATGGAGC ACTGAAAGTG 
GTCCTCGCCT CTGGACGCAC CTTTGAGGCT GTGGAGCAGT TGCTCGAGTC CGCCTCTACT 
AATGCCGATG ATGATGAGTA CCTGCAACTG ATCAACGAGA TCCACTCAAA GGCTATTCCG 
AATCACTTCT TCCGTGGCTA CGGAGTGGTG AGCAGCAAGG GCACCCACCA ACGTGAGGTG 
ATCGAGTCCA ACGACGATAA ACGCCCTATT TGGTACATCT ACTCCGGCAT GGGCAGCCAG 
TGGGCTAGCA TGGCCAAGGA TCTGATGAAG ATCGAGGCCT TCGCCAAGAC CATTCAGAGG 
TGTGCCGATG TCCTAAAGCC CGAGGGAGTG GATCTAATCG ATGTCCTGAC CCGTTCCACG 
GACAAGAGCT TCGAAAACAT TCTGAACTCT TTCATCTCGA TTGCTGCCAT GCAAGTGGCT 
CTTACCGATT TGCTCAGCTC GCTGGGCATC CACCCCGACG GAATTGTGGG ACACTCGGTG 
GGAGAGCTTG GATGCGCCTA CGCCGATGGC TGCTTCACCC CGGAGCAGAC CGTGCTGGCT 
GCTTACTGGC GAGGAAAGAG CATTCTGGAC ACTCAACTGG CCAAGGGCAA GATGGCCGCT 
GTGGGATTGA GCTGGGAAGA TGCGCACAGC CGTGTGCCCA GCGATTGCTT CCCGGTGTGC 
CACAACAGCG AGGACAACTG CACCATCTCT GGCCCGGAGG CTTCCATCGA GGCTCTGGTC 
GCTAAACTGA ACGCGGAGGG AGTGTTCGCC AAGGCAGTGA ACTCATCCGG CTACGCTTTC 
CACAGCAAGT ACATCGCTGA AGCTGGACCC AAACTACGCA AGAGTCTGGA GAAGATCATT 
CCCAACGCTA AGAACCGTAC CGCCCGCTGG ATCAGTACCA GCATCCCAGA ATCCGCTTGG 
AACACACCGG TGGCCAAGCA ATCCTCGGCC GCCTACCACG TGAACAATCT GCTGTCGCCA 
GTGCTCTTCC ACGAGGCCCT TCAGCACGTT CCCAAGAACG CCATTTCCGT GGAGATTGCT 
CCCCACGGCC TGCTCCAGGC TATCCTCAAG CGCGCCCTGG GTCCTGATGC CACCAATCTG 
AGCTTGGTGA AGCGCGGACA CGAGAACAAC GTTGAGTTCT TCCTCACCAA TGTGGGCAAA 
CTCTTTGCCG CCGGAGCCCA GCCTCAGGTA CTTACTCTAG TGCGTCCTAT CAGCTACCCG 
GTGGGTCGTG GAACCCCCAT GTTGAACTCC AAGGTCGGAT GGGACCACAC CCAGAAGTGG 
CTGGTGGCCA AGTTCGGCAA GGAAACTTCT TCTGGTGAGA CCATCGTGGA AGTCGATCTC 
TCCAAGGAGG ATGATGCTTT CCTGGCTGGA CACACGATCG ACGGACGTAT CCTCTTCCCG 
GCCACTGGCT ACATGACTCT GGCTTGGCAG ACATTCGCTA AGATGCAGGG AAGTGAGTTC 
CACAAGACCC CGGTGGTAAT GGAGAACCTC GTGTTCCACC GTGCCACTAT TCTTAACAAG 
AACGCAGTGG TCAAGTTTGG CATTAACTTC TTCGATGGAA CTGGCGCTTT TGAGATTTGT 
GAGAGCGGTA GCTTGGCGGT TTCTGGAAAA ATAACCATTC CAGAGTCTAT TGACAACGAG 
GAGTTGCCCC TGGAGGAGCA AACTCCCAGC GCTGTTGCCA AGGAGCTAGG CACCAACGAT 
GTCTACAAGG AGCTGCGCCT CCGAGGCTAC GACTATGGTG GAATTTTCCG CGGCATTGTT 
CGTTCTGACA CCGTGGCCTC CACTGGAAAG CTTCAGTGGG TGGACAACTG GATTAGTTTC 
ATGGACACCA TGTTGCAGTT CAGTATCCTT AGCAAGAACC TTCGTGAGCT GTACCTGCCC 
ACTCGCATCG AGCGAGCGGT GATTAATCCC GCCAAGCACT TTGAATTGCT GAGCGCCCTC 
ACCAAGGAGG AACAGGTTGA GACTGGACTA CCTGTGCAGT GGTACAGCGA CATTAACGTG 
ATCAAGAGTG CGGGCGTGGA GCTGCGCGGT CTAAAGGCCA ATCTCGCCCA GCGTCGTCCT 
GGCACACAGG CACCTCCCAC TCTGGAGCGC TACCAGTTTG TGCCCAACAT CAACACCACG 
GATCTAAATG AAAACTCTGA GAAGGCTCGC CTGCACGCCT TGGATGTGGC CATCCAGGTG 
ATCATCGAGA ACTCCAGCGG AGCCGTCAAG CTAAAGGGCG TGGAATTGGC CAATGGACGC 
AACCCCGACG TTTTGGTTGC CAACCGCCTG CTGCAGATTA TCGAGGGTGA GCCCGTGCTT 
ACTGGTGATG TGGCTGTGGT CACCTCGAAC AACAATGAAG AGACCATAAC CGCCGCCCTA 
GGAGATTCAG GAGTTCGAGT GGTGTCCAAG GACGTGCTGA AGGAACCGGT AGAGCAGAAC 
TGTCACTTCG TCTTTGGCAT CGATGTCCTT TCCCGACCGG ACACCAAGAC CCTGGAGAAC 
TCCATTGCCA GCATTCGCGA GAACGGTTTC CTGATCCTTG AGGAGACGCT GCCCACTTAC 
ACCAAGACAG GCCGTGCCCT GCTGACCAAA TTTGGATTCG TTGCCGTTCA GGAACAGAGT 
CTGGGAGCCA CCCGTGTCCT GGTCCTCGCC CGCAAGGCTG TTGATCTGAA AACTCGCAAG 
TCCGTTGTGG TTGTGGCCAC CGAGCAGAAC TTTAACTGGG TGGATGACTT GAAGGCCGCT 
CTGGCTACTG CTGCTACGGA GGAGCAGTAT GTGTACGTGG TTTGCCAGGG AGAGGAGCTG 
TTTGGAGCTG TGGGTCTGAT GACGTGCATC AAGAACGAGA ATGGCGGCAA ACTGGCTCGC 
CTGGTCTTCG TGCAAGATGC CAAGGCTGAG AAGTTCTCCC TTACATCGAC CCTGTACCGC 
CAGCAGCTGG AGAAGGACCT CATCTCGAAC GTGCTGAAGA ACGGCGCTTG GGGCACTTTC 
CGTCACCTGA AACTGGAGAC CCAGCAGGCC ACTCTCCAAG TGGAGCATGC CTATGTAAAC 
GCTCTGGTCA AGGGTGATCT TGCTTCCCTC AAGTGGATCG AGGCTGCCCA GGCGGATACC 
GCTGCAACTG TCGACAAGAA CCTGGAAACT TGCACCGTCT ACTATGCGCC CATCAACTTC 
CGTGACGTTA TGTTGACCTC TGGTAAGCTG GCCGCGGATG CCTTGCCTGG AGATCTGGCT 
GAGCAAGATT GTGTGCTGGG TCTGGAGTTC GCTGGTCGCG ATACCCAGGG TCGTCGTGTA 
ATGGCCATGG TGCCGGCTAA ATCACTTGCT ACCACCTGCG TGGCAAGCAA GCGCATGATG 
TGGCAAATCC CTGAGAAGTG GACCATGGAA GAGGCTTCGA CCGTTCCTTG CGTGTACTCC 
ACCGTCTACT ACGCTCTGGT TGTGCGAGGA CAAATGAAGA AGGGTGAGAA GATCCTCATT 
CACGCTGGCT CCGGAGGAGT TGGTCAGGCG GCCATCTCTG TGGCTCTGGC CCATGGCTTG 
ACCGTGTTCA CCACGGTGGG CAGCAAGGAA AAGCGCGAGT TCCTGCTGAA GCGATTCCCC 
AAACTGCAGG AGCGTAACAT CGGCAACTCC CGAGACACCT CCTTCGAGCA GTTGGTCCTG 
CGCGAGACCA AGGGACGCGG AGTCGATCTG GTGCTGAACT CGTTATCTGA AGAGAAACTG 
CAGGCCTCGA TCCGCTGTTT GGGTCTGAAT GGACGCTTCC TGGAGATTGG CAAGTTCGAT 
TTGAGCAACA ACAGCCCACT TGGAATGTCC GTTTTCCTCA AGAACACCTC CTTCCACGGC 
ATTTTGCTTG ACAGTGTGAT GGAGGGCGAG GAGGAGATGC AAAACCAGGT TGTCTCCCTG 
GTCGCTGAGG GCATCAAGAC CGGAGCCGTT GTACCCCTTC CGACCTCGGT GTTTAACGAC 
CAGCAGGTGG AGCAGGCCTT CCGTTTCATG GCCTCTGGCA AGCACATCGG CAAGGTAGTG 
ATCAAGGTGC GTGATGAGGA GGCAGGCAAG AAGGCGCTGC AGCCCAAGCC GCGCCTGATC 
AACGCCATTC CACGCACATA CATGCACCCG GAGAAGAGTT ACATCCTGGT TGGAGGTCTT 
GGCGGATTCG GTTTGGAGCT GACCAACTGG TTGGTGACGC GTGGAGCCCG CTACATTGTG 
CTGACCTCTC GTTCCGGAGT TAAGACCGGC TACCAGGGTC TGATGATCCG TCGCTGGCAG 
GAACGTGGCG TAAAGGTGGT GATTGACACC AGTGATGTGA CCACCGCCGC TGGAGCTAAA 
AAGCTGCTGG AGAATAGTAA CAAGCTGGCT TTGGTCGGAG GCATCTTCAA CCTGGCGGCT 
GTTCTCCGCG ATGCTCTGAT CGAGGATCAG ACCGCCAAGG ACTTCAAGAC AGTGGCTGAT 
CCCAAGGTGA CGGCCACCAA GTACCTGGAT CAGTTCTCTC GCGATATCTG CACTGAGCTG 
GACTACTTCA TCTGCTTCTC CAGTGTTTCC TGCGGTCGTG GTAACATTGG TCAGACCAAC 
TACGGATTGG CCAACTCTGC AATGGAGCGA ATTTGCGAGC AGCGCCAGGT GAGCGGATTC 
CCAGGCACCG CCATCCAATG GGGAGCCATC GGAGACACCG GTCTGGTGCT GGAGAACTTG 
GGTGACAATG ACACCGTGAT TGGAGGAACC CTGCCCCAGA GGATGCCCTC TTGCCTGCAG 
ACCATCGACC TGTTCCTGCA ACAGCCCCAT CCGGTTGTTG CCTCCATGGT TGTGGCCGAG 
AAGCGCAAGT CGGATCAGTC GGCTGGAGTC AGCCTAATTG CCACCATTGC CAACATCCTT 
GGTCTCCGGG ACACCAAGAA TATCCAGGAC GGAGCATCGC TTGCTGATCT CGGAATGGAC 
TCCCTGATGA GCGCTGAGAT CAAACAGACT TTGGAGCGAA ACTTTGACAT TGTTCTGTCC 
GCCCAGGAGA TCCGTCAGCT CACCTTTGGA GCCCTCAAGG CCATGGATGG TGGTGCTGAT 
GTCAAGCCAG CCGCAGCTGC TCCCGCAGCC GCTGCTGGAG TCCCAGAGGC AAACATTACC 
TCTGGTGGAT CCTCGCGCAC AGCTAGTCCC ATGGGCGATG GAACGCAGGT GGTGTTCACC 
ACGTCCCTCA TCCCCACCGA GGCAATTGTA CAACTGGACA CAAAGGCACC TGCAAACAGC 
AAACAGAGCC CCATCTTCTT CATTTCCCCG ATCGAGGGGT TCGCCTCCGC ACTGGAGCCG 
CTGGCCAAGC GATTGGAGGT GCCCGCCTAT GGACTGCAGT ACACGGAAGC CGTGCCCTCC 
GATTCTCTGG AATCAGCAGC CAAATTCTTC ATCAAGCAGC TGCGCACCGT CCAGCCAAAG 
GGTCCCTACA AACTAGCCGG ATACTCCTTC GGTTGCCTGC TCACCTACGT GATGGCCGGT 
ATTCTGGAAG AGACCAACGA GGTGGCCAAT GTGATCATGC TGGACGGAGC CCCAAGCTAC 
GTCAACTGGT ACACGAGCAG CTTCAAGCAG CGTTACACTG ACGGCACCAA CGCCGATAAC 
GACAACCAGA GCTACGGCTT GGCCTACTTT GGAATCGTGT TGGCCAACAT TGACTACAAA 
GCGCTGGTGC GTCTGCTAAT AGTGATCCCC ACATGGGAGG AGAAACTCGA GAGGTTCGCT 
GAGCTGATGA GCAATGAGAT CACCCAGCCC GTGGAAACGA TCAAGAAATC CGCCACTCTG 
TTCTACAAGA AACTGGAGCT GGCCGATGGC TACCAGCCCA CTCTGAAGCT CAAGACGAAT 
GTGACCTTGG TCAAGCCCAC AGACAACTCT GCCAAGCTGG ACGAGGACTA TCGCCTTAAG 
GAGGTCTGCA CAAAGCCCGT GGAAGTACAC ACTGTTGAGG GCAACCACCG CACCTTCCTC 
ATCGAGGATC AGTCCCTGAA GACCATCCAG AGCATACTGA AGCGTCTGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608748.1
CDS	631..7947
Translation	MPARFAEEVITAEPAQRAAPQLDLGGGHYVPRQQHLNDEIAITGFSGRLPESSTIEEFKQNLFDGVDMVNDDPRRWERGLYGLPDRIGKLKDSDLENFDQQFFGVHQKQAECMDPLLRMLLELTHEAIIDAGLNPSDLRGSRTGVYIGVSNSETEQHWCSDADRVNGYGLTGCARAMFANRISFTFDFKGPSYSIDTACSSSLYALEQAFSDMREGKVDNALVAGAGLILKPTMSLQFKRLNMLSPDGSCKAFDESGNGYVRSDGCVVLLLQRTSAARRVYASILNVRTNTDGFKEQGITYPIGKMQNRLIRETYEEIGLNPADVVYVEAHGTGTKVGDPQEVNSITDFFCKDRTTPLLIGSVKSNMGHSEPASGVCSVAKILIAMEEGVIPGNLHYNKPNPDLYGLVDGRLKVVDRNLPWNGGIIGLNSFGFGGANAHVILKSNPKPKALTPKDGALKVVLASGRTFEAVEQLLESASTNADDDEYLQLINEIHSKAIPNHFFRGYGVVSSKGTHQREVIESNDDKRPIWYIYSGMGSQWASMAKDLMKIEAFAKTIQRCADVLKPEGVDLIDVLTRSTDKSFENILNSFISIAAMQVALTDLLSSLGIHPDGIVGHSVGELGCAYADGCFTPEQTVLAAYWRGKSILDTQLAKGKMAAVGLSWEDAHSRVPSDCFPVCHNSEDNCTISGPEASIEALVAKLNAEGVFAKAVNSSGYAFHSKYIAEAGPKLRKSLEKIIPNAKNRTARWISTSIPESAWNTPVAKQSSAAYHVNNLLSPVLFHEALQHVPKNAISVEIAPHGLLQAILKRALGPDATNLSLVKRGHENNVEFFLTNVGKLFAAGAQPQVLTLVRPISYPVGRGTPMLNSKVGWDHTQKWLVAKFGKETSSGETIVEVDLSKEDDAFLAGHTIDGRILFPATGYMTLAWQTFAKMQGSEFHKTPVVMENLVFHRATILNKNAVVKFGINFFDGTGAFEICESGSLAVSGKITIPESIDNEELPLEEQTPSAVAKELGTNDVYKELRLRGYDYGGIFRGIVRSDTVASTGKLQWVDNWISFMDTMLQFSILSKNLRELYLPTRIERAVINPAKHFELLSALTKEEQVETGLPVQWYSDINVIKSAGVELRGLKANLAQRRPGTQAPPTLERYQFVPNINTTDLNENSEKARLHALDVAIQVIIENSSGAVKLKGVELANGRNPDVLVANRLLQIIEGEPVLTGDVAVVTSNNNEETITAALGDSGVRVVSKDVLKEPVEQNCHFVFGIDVLSRPDTKTLENSIASIRENGFLILEETLPTYTKTGRALLTKFGFVAVQEQSLGATRVLVLARKAVDLKTRKSVVVVATEQNFNWVDDLKAALATAATEEQYVYVVCQGEELFGAVGLMTCIKNENGGKLARLVFVQDAKAEKFSLTSTLYRQQLEKDLISNVLKNGAWGTFRHLKLETQQATLQVEHAYVNALVKGDLASLKWIEAAQADTAATVDKNLETCTVYYAPINFRDVMLTSGKLAADALPGDLAEQDCVLGLEFAGRDTQGRRVMAMVPAKSLATTCVASKRMMWQIPEKWTMEEASTVPCVYSTVYYALVVRGQMKKGEKILIHAGSGGVGQAAISVALAHGLTVFTTVGSKEKREFLLKRFPKLQERNIGNSRDTSFEQLVLRETKGRGVDLVLNSLSEEKLQASIRCLGLNGRFLEIGKFDLSNNSPLGMSVFLKNTSFHGILLDSVMEGEEEMQNQVVSLVAEGIKTGAVVPLPTSVFNDQQVEQAFRFMASGKHIGKVVIKVRDEEAGKKALQPKPRLINAIPRTYMHPEKSYILVGGLGGFGLELTNWLVTRGARYIVLTSRSGVKTGYQGLMIRRWQERGVKVVIDTSDVTTAAGAKKLLENSNKLALVGGIFNLAAVLRDALIEDQTAKDFKTVADPKVTATKYLDQFSRDICTELDYFICFSSVSCGRGNIGQTNYGLANSAMERICEQRQVSGFPGTAIQWGAIGDTGLVLENLGDNDTVIGGTLPQRMPSCLQTIDLFLQQPHPVVASMVVAEKRKSDQSAGVSLIATIANILGLRDTKNIQDGASLADLGMDSLMSAEIKQTLERNFDIVLSAQEIRQLTFGALKAMDGGADVKPAAAAPAAAAGVPEANITSGGSSRTASPMGDGTQVVFTTSLIPTEAIVQLDTKAPANSKQSPIFFISPIEGFASALEPLAKRLEVPAYGLQYTEAVPSDSLESAAKFFIKQLRTVQPKGPYKLAGYSFGCLLTYVMAGILEETNEVANVIMLDGAPSYVNWYTSSFKQRYTDGTNADNDNQSYGLAYFGIVLANIDYKALVRLLIVIPTWEEKLERFAELMSNEITQPVETIKKSATLFYKKLELADGYQPTLKLKTNVTLVKPTDNSAKLDEDYRLKEVCTKPVEVHTVEGNHRTFLIEDQSLKTIQSILKRLFN

Target ORF information:

RefSeq Version	NM_134904.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fatty acid synthase 1, transcript variant A (FASN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134904.3

ORF Insert Sequence:

ATGCCCGCCC GATTCGCCGA GGAAGTCATC ACCGCTGAGC CCGCACAACG TGCCGCCCCC 
CAATTGGACC TGGGTGGTGG TCACTATGTG CCCCGTCAGC AGCACCTCAA CGATGAGATC 
GCCATCACCG GTTTCTCCGG CCGTCTGCCG GAGAGCTCCA CCATCGAGGA GTTCAAGCAG 
AATCTTTTCG ACGGCGTCGA CATGGTCAAC GATGATCCCC GCCGTTGGGA GCGTGGTCTG 
TATGGACTTC CCGACAGGAT TGGCAAGCTC AAGGATAGCG ATCTGGAGAA CTTTGACCAA 
CAGTTCTTCG GTGTGCACCA AAAGCAGGCT GAGTGCATGG ACCCACTGCT GCGCATGCTG 
CTCGAGCTCA CCCATGAAGC TATTATTGAC GCTGGCCTAA ACCCAAGTGA TCTGCGCGGT 
TCCCGCACTG GTGTGTACAT CGGTGTGTCC AACTCGGAGA CTGAGCAGCA CTGGTGCAGC 
GATGCTGACC GGGTTAACGG CTACGGCTTG ACAGGATGTG CCCGTGCCAT GTTTGCCAAC 
CGCATCTCCT TCACCTTTGA TTTCAAGGGA CCCAGCTACA GCATTGACAC CGCTTGCTCC 
AGTTCTCTGT ACGCCTTGGA ACAGGCTTTC TCCGATATGC GCGAAGGAAA GGTCGACAAC 
GCCCTGGTCG CTGGAGCTGG ACTGATCCTC AAGCCCACCA TGTCGCTGCA GTTCAAGCGA 
CTGAATATGT TGAGCCCGGA CGGCAGCTGC AAGGCCTTCG ATGAGTCTGG CAATGGATAC 
GTCCGTTCCG ATGGATGTGT GGTGCTGCTC CTGCAGCGCA CCTCTGCAGC CAGGCGTGTG 
TATGCCTCCA TCCTCAATGT GCGCACCAAT ACGGATGGTT TCAAGGAGCA GGGCATCACA 
TACCCTATTG GCAAGATGCA AAATCGCCTG ATCCGCGAGA CCTACGAGGA GATTGGTCTT 
AACCCCGCCG ATGTGGTTTA CGTGGAGGCA CACGGTACCG GAACCAAGGT GGGCGATCCC 
CAGGAGGTGA ACTCTATCAC TGACTTCTTC TGCAAGGACC GTACGACCCC TCTGTTGATC 
GGATCGGTCA AGTCCAACAT GGGTCACTCG GAACCCGCTT CCGGTGTCTG CTCTGTGGCC 
AAGATTCTGA TCGCCATGGA GGAGGGCGTC ATTCCCGGTA ACTTGCACTA CAACAAGCCG 
AACCCAGATC TTTATGGACT CGTTGATGGA CGTCTCAAGG TGGTCGACAG GAATCTGCCT 
TGGAACGGTG GCATCATCGG CCTGAACTCC TTTGGTTTTG GAGGAGCCAA CGCTCACGTT 
ATCTTAAAGT CGAACCCCAA GCCCAAGGCT TTGACCCCCA AGGATGGAGC ACTGAAAGTG 
GTCCTCGCCT CTGGACGCAC CTTTGAGGCT GTGGAGCAGT TGCTCGAGTC CGCCTCTACT 
AATGCCGATG ATGATGAGTA CCTGCAACTG ATCAACGAGA TCCACTCAAA GGCTATTCCG 
AATCACTTCT TCCGTGGCTA CGGAGTGGTG AGCAGCAAGG GCACCCACCA ACGTGAGGTG 
ATCGAGTCCA ACGACGATAA ACGCCCTATT TGGTACATCT ACTCCGGCAT GGGCAGCCAG 
TGGGCTAGCA TGGCCAAGGA TCTGATGAAG ATCGAGGCCT TCGCCAAGAC CATTCAGAGG 
TGTGCCGATG TCCTAAAGCC CGAGGGAGTG GATCTAATCG ATGTCCTGAC CCGTTCCACG 
GACAAGAGCT TCGAAAACAT TCTGAACTCT TTCATCTCGA TTGCTGCCAT GCAAGTGGCT 
CTTACCGATT TGCTCAGCTC GCTGGGCATC CACCCCGACG GAATTGTGGG ACACTCGGTG 
GGAGAGCTTG GATGCGCCTA CGCCGATGGC TGCTTCACCC CGGAGCAGAC CGTGCTGGCT 
GCTTACTGGC GAGGAAAGAG CATTCTGGAC ACTCAACTGG CCAAGGGCAA GATGGCCGCT 
GTGGGATTGA GCTGGGAAGA TGCGCACAGC CGTGTGCCCA GCGATTGCTT CCCGGTGTGC 
CACAACAGCG AGGACAACTG CACCATCTCT GGCCCGGAGG CTTCCATCGA GGCTCTGGTC 
GCTAAACTGA ACGCGGAGGG AGTGTTCGCC AAGGCAGTGA ACTCATCCGG CTACGCTTTC 
CACAGCAAGT ACATCGCTGA AGCTGGACCC AAACTACGCA AGAGTCTGGA GAAGATCATT 
CCCAACGCTA AGAACCGTAC CGCCCGCTGG ATCAGTACCA GCATCCCAGA ATCCGCTTGG 
AACACACCGG TGGCCAAGCA ATCCTCGGCC GCCTACCACG TGAACAATCT GCTGTCGCCA 
GTGCTCTTCC ACGAGGCCCT TCAGCACGTT CCCAAGAACG CCATTTCCGT GGAGATTGCT 
CCCCACGGCC TGCTCCAGGC TATCCTCAAG CGCGCCCTGG GTCCTGATGC CACCAATCTG 
AGCTTGGTGA AGCGCGGACA CGAGAACAAC GTTGAGTTCT TCCTCACCAA TGTGGGCAAA 
CTCTTTGCCG CCGGAGCCCA GCCTCAGGTA CTTACTCTAG TGCGTCCTAT CAGCTACCCG 
GTGGGTCGTG GAACCCCCAT GTTGAACTCC AAGGTCGGAT GGGACCACAC CCAGAAGTGG 
CTGGTGGCCA AGTTCGGCAA GGAAACTTCT TCTGGTGAGA CCATCGTGGA AGTCGATCTC 
TCCAAGGAGG ATGATGCTTT CCTGGCTGGA CACACGATCG ACGGACGTAT CCTCTTCCCG 
GCCACTGGCT ACATGACTCT GGCTTGGCAG ACATTCGCTA AGATGCAGGG AAGTGAGTTC 
CACAAGACCC CGGTGGTAAT GGAGAACCTC GTGTTCCACC GTGCCACTAT TCTTAACAAG 
AACGCAGTGG TCAAGTTTGG CATTAACTTC TTCGATGGAA CTGGCGCTTT TGAGATTTGT 
GAGAGCGGTA GCTTGGCGGT TTCTGGAAAA ATAACCATTC CAGAGTCTAT TGACAACGAG 
GAGTTGCCCC TGGAGGAGCA AACTCCCAGC GCTGTTGCCA AGGAGCTAGG CACCAACGAT 
GTCTACAAGG AGCTGCGCCT CCGAGGCTAC GACTATGGTG GAATTTTCCG CGGCATTGTT 
CGTTCTGACA CCGTGGCCTC CACTGGAAAG CTTCAGTGGG TGGACAACTG GATTAGTTTC 
ATGGACACCA TGTTGCAGTT CAGTATCCTT AGCAAGAACC TTCGTGAGCT GTACCTGCCC 
ACTCGCATCG AGCGAGCGGT GATTAATCCC GCCAAGCACT TTGAATTGCT GAGCGCCCTC 
ACCAAGGAGG AACAGGTTGA GACTGGACTA CCTGTGCAGT GGTACAGCGA CATTAACGTG 
ATCAAGAGTG CGGGCGTGGA GCTGCGCGGT CTAAAGGCCA ATCTCGCCCA GCGTCGTCCT 
GGCACACAGG CACCTCCCAC TCTGGAGCGC TACCAGTTTG TGCCCAACAT CAACACCACG 
GATCTAAATG AAAACTCTGA GAAGGCTCGC CTGCACGCCT TGGATGTGGC CATCCAGGTG 
ATCATCGAGA ACTCCAGCGG AGCCGTCAAG CTAAAGGGCG TGGAATTGGC CAATGGACGC 
AACCCCGACG TTTTGGTTGC CAACCGCCTG CTGCAGATTA TCGAGGGTGA GCCCGTGCTT 
ACTGGTGATG TGGCTGTGGT CACCTCGAAC AACAATGAAG AGACCATAAC CGCCGCCCTA 
GGAGATTCAG GAGTTCGAGT GGTGTCCAAG GACGTGCTGA AGGAACCGGT AGAGCAGAAC 
TGTCACTTCG TCTTTGGCAT CGATGTCCTT TCCCGACCGG ACACCAAGAC CCTGGAGAAC 
TCCATTGCCA GCATTCGCGA GAACGGTTTC CTGATCCTTG AGGAGACGCT GCCCACTTAC 
ACCAAGACAG GCCGTGCCCT GCTGACCAAA TTTGGATTCG TTGCCGTTCA GGAACAGAGT 
CTGGGAGCCA CCCGTGTCCT GGTCCTCGCC CGCAAGGCTG TTGATCTGAA AACTCGCAAG 
TCCGTTGTGG TTGTGGCCAC CGAGCAGAAC TTTAACTGGG TGGATGACTT GAAGGCCGCT 
CTGGCTACTG CTGCTACGGA GGAGCAGTAT GTGTACGTGG TTTGCCAGGG AGAGGAGCTG 
TTTGGAGCTG TGGGTCTGAT GACGTGCATC AAGAACGAGA ATGGCGGCAA ACTGGCTCGC 
CTGGTCTTCG TGCAAGATGC CAAGGCTGAG AAGTTCTCCC TTACATCGAC CCTGTACCGC 
CAGCAGCTGG AGAAGGACCT CATCTCGAAC GTGCTGAAGA ACGGCGCTTG GGGCACTTTC 
CGTCACCTGA AACTGGAGAC CCAGCAGGCC ACTCTCCAAG TGGAGCATGC CTATGTAAAC 
GCTCTGGTCA AGGGTGATCT TGCTTCCCTC AAGTGGATCG AGGCTGCCCA GGCGGATACC 
GCTGCAACTG TCGACAAGAA CCTGGAAACT TGCACCGTCT ACTATGCGCC CATCAACTTC 
CGTGACGTTA TGTTGACCTC TGGTAAGCTG GCCGCGGATG CCTTGCCTGG AGATCTGGCT 
GAGCAAGATT GTGTGCTGGG TCTGGAGTTC GCTGGTCGCG ATACCCAGGG TCGTCGTGTA 
ATGGCCATGG TGCCGGCTAA ATCACTTGCT ACCACCTGCG TGGCAAGCAA GCGCATGATG 
TGGCAAATCC CTGAGAAGTG GACCATGGAA GAGGCTTCGA CCGTTCCTTG CGTGTACTCC 
ACCGTCTACT ACGCTCTGGT TGTGCGAGGA CAAATGAAGA AGGGTGAGAA GATCCTCATT 
CACGCTGGCT CCGGAGGAGT TGGTCAGGCG GCCATCTCTG TGGCTCTGGC CCATGGCTTG 
ACCGTGTTCA CCACGGTGGG CAGCAAGGAA AAGCGCGAGT TCCTGCTGAA GCGATTCCCC 
AAACTGCAGG AGCGTAACAT CGGCAACTCC CGAGACACCT CCTTCGAGCA GTTGGTCCTG 
CGCGAGACCA AGGGACGCGG AGTCGATCTG GTGCTGAACT CGTTATCTGA AGAGAAACTG 
CAGGCCTCGA TCCGCTGTTT GGGTCTGAAT GGACGCTTCC TGGAGATTGG CAAGTTCGAT 
TTGAGCAACA ACAGCCCACT TGGAATGTCC GTTTTCCTCA AGAACACCTC CTTCCACGGC 
ATTTTGCTTG ACAGTGTGAT GGAGGGCGAG GAGGAGATGC AAAACCAGGT TGTCTCCCTG 
GTCGCTGAGG GCATCAAGAC CGGAGCCGTT GTACCCCTTC CGACCTCGGT GTTTAACGAC 
CAGCAGGTGG AGCAGGCCTT CCGTTTCATG GCCTCTGGCA AGCACATCGG CAAGGTAGTG 
ATCAAGGTGC GTGATGAGGA GGCAGGCAAG AAGGCGCTGC AGCCCAAGCC GCGCCTGATC 
AACGCCATTC CACGCACATA CATGCACCCG GAGAAGAGTT ACATCCTGGT TGGAGGTCTT 
GGCGGATTCG GTTTGGAGCT GACCAACTGG TTGGTGACGC GTGGAGCCCG CTACATTGTG 
CTGACCTCTC GTTCCGGAGT TAAGACCGGC TACCAGGGTC TGATGATCCG TCGCTGGCAG 
GAACGTGGCG TAAAGGTGGT GATTGACACC AGTGATGTGA CCACCGCCGC TGGAGCTAAA 
AAGCTGCTGG AGAATAGTAA CAAGCTGGCT TTGGTCGGAG GCATCTTCAA CCTGGCGGCT 
GTTCTCCGCG ATGCTCTGAT CGAGGATCAG ACCGCCAAGG ACTTCAAGAC AGTGGCTGAT 
CCCAAGGTGA CGGCCACCAA GTACCTGGAT CAGTTCTCTC GCGATATCTG CACTGAGCTG 
GACTACTTCA TCTGCTTCTC CAGTGTTTCC TGCGGTCGTG GTAACATTGG TCAGACCAAC 
TACGGATTGG CCAACTCTGC AATGGAGCGA ATTTGCGAGC AGCGCCAGGT GAGCGGATTC 
CCAGGCACCG CCATCCAATG GGGAGCCATC GGAGACACCG GTCTGGTGCT GGAGAACTTG 
GGTGACAATG ACACCGTGAT TGGAGGAACC CTGCCCCAGA GGATGCCCTC TTGCCTGCAG 
ACCATCGACC TGTTCCTGCA ACAGCCCCAT CCGGTTGTTG CCTCCATGGT TGTGGCCGAG 
AAGCGCAAGT CGGATCAGTC GGCTGGAGTC AGCCTAATTG CCACCATTGC CAACATCCTT 
GGTCTCCGGG ACACCAAGAA TATCCAGGAC GGAGCATCGC TTGCTGATCT CGGAATGGAC 
TCCCTGATGA GCGCTGAGAT CAAACAGACT TTGGAGCGAA ACTTTGACAT TGTTCTGTCC 
GCCCAGGAGA TCCGTCAGCT CACCTTTGGA GCCCTCAAGG CCATGGATGG TGGTGCTGAT 
GTCAAGCCAG CCGCAGCTGC TCCCGCAGCC GCTGCTGGAG TCCCAGAGGC AAACATTACC 
TCTGGTGGAT CCTCGCGCAC AGCTAGTCCC ATGGGCGATG GAACGCAGGT GGTGTTCACC 
ACGTCCCTCA TCCCCACCGA GGCAATTGTA CAACTGGACA CAAAGGCACC TGCAAACAGC 
AAACAGAGCC CCATCTTCTT CATTTCCCCG ATCGAGGGGT TCGCCTCCGC ACTGGAGCCG 
CTGGCCAAGC GATTGGAGGT GCCCGCCTAT GGACTGCAGT ACACGGAAGC CGTGCCCTCC 
GATTCTCTGG AATCAGCAGC CAAATTCTTC ATCAAGCAGC TGCGCACCGT CCAGCCAAAG 
GGTCCCTACA AACTAGCCGG ATACTCCTTC GGTTGCCTGC TCACCTACGT GATGGCCGGT 
ATTCTGGAAG AGACCAACGA GGTGGCCAAT GTGATCATGC TGGACGGAGC CCCAAGCTAC 
GTCAACTGGT ACACGAGCAG CTTCAAGCAG CGTTACACTG ACGGCACCAA CGCCGATAAC 
GACAACCAGA GCTACGGCTT GGCCTACTTT GGAATCGTGT TGGCCAACAT TGACTACAAA 
GCGCTGGTGC GTCTGCTAAT AGTGATCCCC ACATGGGAGG AGAAACTCGA GAGGTTCGCT 
GAGCTGATGA GCAATGAGAT CACCCAGCCC GTGGAAACGA TCAAGAAATC CGCCACTCTG 
TTCTACAAGA AACTGGAGCT GGCCGATGGC TACCAGCCCA CTCTGAAGCT CAAGACGAAT 
GTGACCTTGG TCAAGCCCAC AGACAACTCT GCCAAGCTGG ACGAGGACTA TCGCCTTAAG 
GAGGTCTGCA CAAAGCCCGT GGAAGTACAC ACTGTTGAGG GCAACCACCG CACCTTCCTC 
ATCGAGGATC AGTCCCTGAA GACCATCCAG AGCATACTGA AGCGTCTGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FASN1 ( NM_001144306.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137778.2 Drosophila melanogaster fatty acid synthase 1, transcript variant C (FASN1), mRNA.

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CloneID	OFa13601
Clone ID Related Accession (Same CDS sequence)	NM_001144306.2
Accession Version	NM_001144306.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fatty acid synthase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144306.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCTAC AACTAAACCT GTTGCAAGCC GTGGACCAGC CGCATCCTGT TGCTTTCAAG 
CCCAACAATT GCAGCACCCA TCAGCCCCAA CATTCACCCA GCACCCAGAA CAACGGAAAG 
AGCGCTCGCG CCAATCGTCG TCGACGCCGC AGACGCAACA AGCAGCAGCA GAATTCGCAG 
CAACCTGACG CCTTCCGTGA TACCGACGCG CAGCTAACAC CGAGCACCTC CTCGTCCACC 
CCGCACTCTG CACCCAACGA TAGACAGGAC CACCTAAACT CCCAATTCAA TCCCGAATCC 
ATCAATATGC CCGCCCGATT CGCCGAGGAA GTCATCACCG CTGAGCCCGC ACAACGTGCC 
GCCCCCCAAT TGGACCTGGG TGGTGGTCAC TATGTGCCCC GTCAGCAGCA CCTCAACGAT 
GAGATCGCCA TCACCGGTTT CTCCGGCCGT CTGCCGGAGA GCTCCACCAT CGAGGAGTTC 
AAGCAGAATC TTTTCGACGG CGTCGACATG GTCAACGATG ATCCCCGCCG TTGGGAGCGT 
GGTCTGTATG GACTTCCCGA CAGGATTGGC AAGCTCAAGG ATAGCGATCT GGAGAACTTT 
GACCAACAGT TCTTCGGTGT GCACCAAAAG CAGGCTGAGT GCATGGACCC ACTGCTGCGC 
ATGCTGCTCG AGCTCACCCA TGAAGCTATT ATTGACGCTG GCCTAAACCC AAGTGATCTG 
CGCGGTTCCC GCACTGGTGT GTACATCGGT GTGTCCAACT CGGAGACTGA GCAGCACTGG 
TGCAGCGATG CTGACCGGGT TAACGGCTAC GGCTTGACAG GATGTGCCCG TGCCATGTTT 
GCCAACCGCA TCTCCTTCAC CTTTGATTTC AAGGGACCCA GCTACAGCAT TGACACCGCT 
TGCTCCAGTT CTCTGTACGC CTTGGAACAG GCTTTCTCCG ATATGCGCGA AGGAAAGGTC 
GACAACGCCC TGGTCGCTGG AGCTGGACTG ATCCTCAAGC CCACCATGTC GCTGCAGTTC 
AAGCGACTGA ATATGTTGAG CCCGGACGGC AGCTGCAAGG CCTTCGATGA GTCTGGCAAT 
GGATACGTCC GTTCCGATGG ATGTGTGGTG CTGCTCCTGC AGCGCACCTC TGCAGCCAGG 
CGTGTGTATG CCTCCATCCT CAATGTGCGC ACCAATACGG ATGGTTTCAA GGAGCAGGGC 
ATCACATACC CTATTGGCAA GATGCAAAAT CGCCTGATCC GCGAGACCTA CGAGGAGATT 
GGTCTTAACC CCGCCGATGT GGTTTACGTG GAGGCACACG GTACCGGAAC CAAGGTGGGC 
GATCCCCAGG AGGTGAACTC TATCACTGAC TTCTTCTGCA AGGACCGTAC GACCCCTCTG 
TTGATCGGAT CGGTCAAGTC CAACATGGGT CACTCGGAAC CCGCTTCCGG TGTCTGCTCT 
GTGGCCAAGA TTCTGATCGC CATGGAGGAG GGCGTCATTC CCGGTAACTT GCACTACAAC 
AAGCCGAACC CAGATCTTTA TGGACTCGTT GATGGACGTC TCAAGGTGGT CGACAGGAAT 
CTGCCTTGGA ACGGTGGCAT CATCGGCCTG AACTCCTTTG GTTTTGGAGG AGCCAACGCT 
CACGTTATCT TAAAGTCGAA CCCCAAGCCC AAGGCTTTGA CCCCCAAGGA TGGAGCACTG 
AAAGTGGTCC TCGCCTCTGG ACGCACCTTT GAGGCTGTGG AGCAGTTGCT CGAGTCCGCC 
TCTACTAATG CCGATGATGA TGAGTACCTG CAACTGATCA ACGAGATCCA CTCAAAGGCT 
ATTCCGAATC ACTTCTTCCG TGGCTACGGA GTGGTGAGCA GCAAGGGCAC CCACCAACGT 
GAGGTGATCG AGTCCAACGA CGATAAACGC CCTATTTGGT ACATCTACTC CGGCATGGGC 
AGCCAGTGGG CTAGCATGGC CAAGGATCTG ATGAAGATCG AGGCCTTCGC CAAGACCATT 
CAGAGGTGTG CCGATGTCCT AAAGCCCGAG GGAGTGGATC TAATCGATGT CCTGACCCGT 
TCCACGGACA AGAGCTTCGA AAACATTCTG AACTCTTTCA TCTCGATTGC TGCCATGCAA 
GTGGCTCTTA CCGATTTGCT CAGCTCGCTG GGCATCCACC CCGACGGAAT TGTGGGACAC 
TCGGTGGGAG AGCTTGGATG CGCCTACGCC GATGGCTGCT TCACCCCGGA GCAGACCGTG 
CTGGCTGCTT ACTGGCGAGG AAAGAGCATT CTGGACACTC AACTGGCCAA GGGCAAGATG 
GCCGCTGTGG GATTGAGCTG GGAAGATGCG CACAGCCGTG TGCCCAGCGA TTGCTTCCCG 
GTGTGCCACA ACAGCGAGGA CAACTGCACC ATCTCTGGCC CGGAGGCTTC CATCGAGGCT 
CTGGTCGCTA AACTGAACGC GGAGGGAGTG TTCGCCAAGG CAGTGAACTC ATCCGGCTAC 
GCTTTCCACA GCAAGTACAT CGCTGAAGCT GGACCCAAAC TACGCAAGAG TCTGGAGAAG 
ATCATTCCCA ACGCTAAGAA CCGTACCGCC CGCTGGATCA GTACCAGCAT CCCAGAATCC 
GCTTGGAACA CACCGGTGGC CAAGCAATCC TCGGCCGCCT ACCACGTGAA CAATCTGCTG 
TCGCCAGTGC TCTTCCACGA GGCCCTTCAG CACGTTCCCA AGAACGCCAT TTCCGTGGAG 
ATTGCTCCCC ACGGCCTGCT CCAGGCTATC CTCAAGCGCG CCCTGGGTCC TGATGCCACC 
AATCTGAGCT TGGTGAAGCG CGGACACGAG AACAACGTTG AGTTCTTCCT CACCAATGTG 
GGCAAACTCT TTGCCGCCGG AGCCCAGCCT CAGGTACTTA CTCTAGTGCG TCCTATCAGC 
TACCCGGTGG GTCGTGGAAC CCCCATGTTG AACTCCAAGG TCGGATGGGA CCACACCCAG 
AAGTGGCTGG TGGCCAAGTT CGGCAAGGAA ACTTCTTCTG GTGAGACCAT CGTGGAAGTC 
GATCTCTCCA AGGAGGATGA TGCTTTCCTG GCTGGACACA CGATCGACGG ACGTATCCTC 
TTCCCGGCCA CTGGCTACAT GACTCTGGCT TGGCAGACAT TCGCTAAGAT GCAGGGAAGT 
GAGTTCCACA AGACCCCGGT GGTAATGGAG AACCTCGTGT TCCACCGTGC CACTATTCTT 
AACAAGAACG CAGTGGTCAA GTTTGGCATT AACTTCTTCG ATGGAACTGG CGCTTTTGAG 
ATTTGTGAGA GCGGTAGCTT GGCGGTTTCT GGAAAAATAA CCATTCCAGA GTCTATTGAC 
AACGAGGAGT TGCCCCTGGA GGAGCAAACT CCCAGCGCTG TTGCCAAGGA GCTAGGCACC 
AACGATGTCT ACAAGGAGCT GCGCCTCCGA GGCTACGACT ATGGTGGAAT TTTCCGCGGC 
ATTGTTCGTT CTGACACCGT GGCCTCCACT GGAAAGCTTC AGTGGGTGGA CAACTGGATT 
AGTTTCATGG ACACCATGTT GCAGTTCAGT ATCCTTAGCA AGAACCTTCG TGAGCTGTAC 
CTGCCCACTC GCATCGAGCG AGCGGTGATT AATCCCGCCA AGCACTTTGA ATTGCTGAGC 
GCCCTCACCA AGGAGGAACA GGTTGAGACT GGACTACCTG TGCAGTGGTA CAGCGACATT 
AACGTGATCA AGAGTGCGGG CGTGGAGCTG CGCGGTCTAA AGGCCAATCT CGCCCAGCGT 
CGTCCTGGCA CACAGGCACC TCCCACTCTG GAGCGCTACC AGTTTGTGCC CAACATCAAC 
ACCACGGATC TAAATGAAAA CTCTGAGAAG GCTCGCCTGC ACGCCTTGGA TGTGGCCATC 
CAGGTGATCA TCGAGAACTC CAGCGGAGCC GTCAAGCTAA AGGGCGTGGA ATTGGCCAAT 
GGACGCAACC CCGACGTTTT GGTTGCCAAC CGCCTGCTGC AGATTATCGA GGGTGAGCCC 
GTGCTTACTG GTGATGTGGC TGTGGTCACC TCGAACAACA ATGAAGAGAC CATAACCGCC 
GCCCTAGGAG ATTCAGGAGT TCGAGTGGTG TCCAAGGACG TGCTGAAGGA ACCGGTAGAG 
CAGAACTGTC ACTTCGTCTT TGGCATCGAT GTCCTTTCCC GACCGGACAC CAAGACCCTG 
GAGAACTCCA TTGCCAGCAT TCGCGAGAAC GGTTTCCTGA TCCTTGAGGA GACGCTGCCC 
ACTTACACCA AGACAGGCCG TGCCCTGCTG ACCAAATTTG GATTCGTTGC CGTTCAGGAA 
CAGAGTCTGG GAGCCACCCG TGTCCTGGTC CTCGCCCGCA AGGCTGTTGA TCTGAAAACT 
CGCAAGTCCG TTGTGGTTGT GGCCACCGAG CAGAACTTTA ACTGGGTGGA TGACTTGAAG 
GCCGCTCTGG CTACTGCTGC TACGGAGGAG CAGTATGTGT ACGTGGTTTG CCAGGGAGAG 
GAGCTGTTTG GAGCTGTGGG TCTGATGACG TGCATCAAGA ACGAGAATGG CGGCAAACTG 
GCTCGCCTGG TCTTCGTGCA AGATGCCAAG GCTGAGAAGT TCTCCCTTAC ATCGACCCTG 
TACCGCCAGC AGCTGGAGAA GGACCTCATC TCGAACGTGC TGAAGAACGG CGCTTGGGGC 
ACTTTCCGTC ACCTGAAACT GGAGACCCAG CAGGCCACTC TCCAAGTGGA GCATGCCTAT 
GTAAACGCTC TGGTCAAGGG TGATCTTGCT TCCCTCAAGT GGATCGAGGC TGCCCAGGCG 
GATACCGCTG CAACTGTCGA CAAGAACCTG GAAACTTGCA CCGTCTACTA TGCGCCCATC 
AACTTCCGTG ACGTTATGTT GACCTCTGGT AAGCTGGCCG CGGATGCCTT GCCTGGAGAT 
CTGGCTGAGC AAGATTGTGT GCTGGGTCTG GAGTTCGCTG GTCGCGATAC CCAGGGTCGT 
CGTGTAATGG CCATGGTGCC GGCTAAATCA CTTGCTACCA CCTGCGTGGC AAGCAAGCGC 
ATGATGTGGC AAATCCCTGA GAAGTGGACC ATGGAAGAGG CTTCGACCGT TCCTTGCGTG 
TACTCCACCG TCTACTACGC TCTGGTTGTG CGAGGACAAA TGAAGAAGGG TGAGAAGATC 
CTCATTCACG CTGGCTCCGG AGGAGTTGGT CAGGCGGCCA TCTCTGTGGC TCTGGCCCAT 
GGCTTGACCG TGTTCACCAC GGTGGGCAGC AAGGAAAAGC GCGAGTTCCT GCTGAAGCGA 
TTCCCCAAAC TGCAGGAGCG TAACATCGGC AACTCCCGAG ACACCTCCTT CGAGCAGTTG 
GTCCTGCGCG AGACCAAGGG ACGCGGAGTC GATCTGGTGC TGAACTCGTT ATCTGAAGAG 
AAACTGCAGG CCTCGATCCG CTGTTTGGGT CTGAATGGAC GCTTCCTGGA GATTGGCAAG 
TTCGATTTGA GCAACAACAG CCCACTTGGA ATGTCCGTTT TCCTCAAGAA CACCTCCTTC 
CACGGCATTT TGCTTGACAG TGTGATGGAG GGCGAGGAGG AGATGCAAAA CCAGGTTGTC 
TCCCTGGTCG CTGAGGGCAT CAAGACCGGA GCCGTTGTAC CCCTTCCGAC CTCGGTGTTT 
AACGACCAGC AGGTGGAGCA GGCCTTCCGT TTCATGGCCT CTGGCAAGCA CATCGGCAAG 
GTAGTGATCA AGGTGCGTGA TGAGGAGGCA GGCAAGAAGG CGCTGCAGCC CAAGCCGCGC 
CTGATCAACG CCATTCCACG CACATACATG CACCCGGAGA AGAGTTACAT CCTGGTTGGA 
GGTCTTGGCG GATTCGGTTT GGAGCTGACC AACTGGTTGG TGACGCGTGG AGCCCGCTAC 
ATTGTGCTGA CCTCTCGTTC CGGAGTTAAG ACCGGCTACC AGGGTCTGAT GATCCGTCGC 
TGGCAGGAAC GTGGCGTAAA GGTGGTGATT GACACCAGTG ATGTGACCAC CGCCGCTGGA 
GCTAAAAAGC TGCTGGAGAA TAGTAACAAG CTGGCTTTGG TCGGAGGCAT CTTCAACCTG 
GCGGCTGTTC TCCGCGATGC TCTGATCGAG GATCAGACCG CCAAGGACTT CAAGACAGTG 
GCTGATCCCA AGGTGACGGC CACCAAGTAC CTGGATCAGT TCTCTCGCGA TATCTGCACT 
GAGCTGGACT ACTTCATCTG CTTCTCCAGT GTTTCCTGCG GTCGTGGTAA CATTGGTCAG 
ACCAACTACG GATTGGCCAA CTCTGCAATG GAGCGAATTT GCGAGCAGCG CCAGGTGAGC 
GGATTCCCAG GCACCGCCAT CCAATGGGGA GCCATCGGAG ACACCGGTCT GGTGCTGGAG 
AACTTGGGTG ACAATGACAC CGTGATTGGA GGAACCCTGC CCCAGAGGAT GCCCTCTTGC 
CTGCAGACCA TCGACCTGTT CCTGCAACAG CCCCATCCGG TTGTTGCCTC CATGGTTGTG 
GCCGAGAAGC GCAAGTCGGA TCAGTCGGCT GGAGTCAGCC TAATTGCCAC CATTGCCAAC 
ATCCTTGGTC TCCGGGACAC CAAGAATATC CAGGACGGAG CATCGCTTGC TGATCTCGGA 
ATGGACTCCC TGATGAGCGC TGAGATCAAA CAGACTTTGG AGCGAAACTT TGACATTGTT 
CTGTCCGCCC AGGAGATCCG TCAGCTCACC TTTGGAGCCC TCAAGGCCAT GGATGGTGGT 
GCTGATGTCA AGCCAGCCGC AGCTGCTCCC GCAGCCGCTG CTGGAGTCCC AGAGGCAAAC 
ATTACCTCTG GTGGATCCTC GCGCACAGCT AGTCCCATGG GCGATGGAAC GCAGGTGGTG 
TTCACCACGT CCCTCATCCC CACCGAGGCA ATTGTACAAC TGGACACAAA GGCACCTGCA 
AACAGCAAAC AGAGCCCCAT CTTCTTCATT TCCCCGATCG AGGGGTTCGC CTCCGCACTG 
GAGCCGCTGG CCAAGCGATT GGAGGTGCCC GCCTATGGAC TGCAGTACAC GGAAGCCGTG 
CCCTCCGATT CTCTGGAATC AGCAGCCAAA TTCTTCATCA AGCAGCTGCG CACCGTCCAG 
CCAAAGGGTC CCTACAAACT AGCCGGATAC TCCTTCGGTT GCCTGCTCAC CTACGTGATG 
GCCGGTATTC TGGAAGAGAC CAACGAGGTG GCCAATGTGA TCATGCTGGA CGGAGCCCCA 
AGCTACGTCA ACTGGTACAC GAGCAGCTTC AAGCAGCGTT ACACTGACGG CACCAACGCC 
GATAACGACA ACCAGAGCTA CGGCTTGGCC TACTTTGGAA TCGTGTTGGC CAACATTGAC 
TACAAAGCGC TGGTGCGTCT GCTAATAGTG ATCCCCACAT GGGAGGAGAA ACTCGAGAGG 
TTCGCTGAGC TGATGAGCAA TGAGATCACC CAGCCCGTGG AAACGATCAA GAAATCCGCC 
ACTCTGTTCT ACAAGAAACT GGAGCTGGCC GATGGCTACC AGCCCACTCT GAAGCTCAAG 
ACGAATGTGA CCTTGGTCAA GCCCACAGAC AACTCTGCCA AGCTGGACGA GGACTATCGC 
CTTAAGGAGG TCTGCACAAA GCCCGTGGAA GTACACACTG TTGAGGGCAA CCACCGCACC 
TTCCTCATCG AGGATCAGTC CCTGAAGACC ATCCAGAGCA TACTGAAGCG TCTGTTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137778.2
CDS	120..7742
Translation	MPLQLNLLQAVDQPHPVAFKPNNCSTHQPQHSPSTQNNGKSARANRRRRRRRNKQQQNSQQPDAFRDTDAQLTPSTSSSTPHSAPNDRQDHLNSQFNPESINMPARFAEEVITAEPAQRAAPQLDLGGGHYVPRQQHLNDEIAITGFSGRLPESSTIEEFKQNLFDGVDMVNDDPRRWERGLYGLPDRIGKLKDSDLENFDQQFFGVHQKQAECMDPLLRMLLELTHEAIIDAGLNPSDLRGSRTGVYIGVSNSETEQHWCSDADRVNGYGLTGCARAMFANRISFTFDFKGPSYSIDTACSSSLYALEQAFSDMREGKVDNALVAGAGLILKPTMSLQFKRLNMLSPDGSCKAFDESGNGYVRSDGCVVLLLQRTSAARRVYASILNVRTNTDGFKEQGITYPIGKMQNRLIRETYEEIGLNPADVVYVEAHGTGTKVGDPQEVNSITDFFCKDRTTPLLIGSVKSNMGHSEPASGVCSVAKILIAMEEGVIPGNLHYNKPNPDLYGLVDGRLKVVDRNLPWNGGIIGLNSFGFGGANAHVILKSNPKPKALTPKDGALKVVLASGRTFEAVEQLLESASTNADDDEYLQLINEIHSKAIPNHFFRGYGVVSSKGTHQREVIESNDDKRPIWYIYSGMGSQWASMAKDLMKIEAFAKTIQRCADVLKPEGVDLIDVLTRSTDKSFENILNSFISIAAMQVALTDLLSSLGIHPDGIVGHSVGELGCAYADGCFTPEQTVLAAYWRGKSILDTQLAKGKMAAVGLSWEDAHSRVPSDCFPVCHNSEDNCTISGPEASIEALVAKLNAEGVFAKAVNSSGYAFHSKYIAEAGPKLRKSLEKIIPNAKNRTARWISTSIPESAWNTPVAKQSSAAYHVNNLLSPVLFHEALQHVPKNAISVEIAPHGLLQAILKRALGPDATNLSLVKRGHENNVEFFLTNVGKLFAAGAQPQVLTLVRPISYPVGRGTPMLNSKVGWDHTQKWLVAKFGKETSSGETIVEVDLSKEDDAFLAGHTIDGRILFPATGYMTLAWQTFAKMQGSEFHKTPVVMENLVFHRATILNKNAVVKFGINFFDGTGAFEICESGSLAVSGKITIPESIDNEELPLEEQTPSAVAKELGTNDVYKELRLRGYDYGGIFRGIVRSDTVASTGKLQWVDNWISFMDTMLQFSILSKNLRELYLPTRIERAVINPAKHFELLSALTKEEQVETGLPVQWYSDINVIKSAGVELRGLKANLAQRRPGTQAPPTLERYQFVPNINTTDLNENSEKARLHALDVAIQVIIENSSGAVKLKGVELANGRNPDVLVANRLLQIIEGEPVLTGDVAVVTSNNNEETITAALGDSGVRVVSKDVLKEPVEQNCHFVFGIDVLSRPDTKTLENSIASIRENGFLILEETLPTYTKTGRALLTKFGFVAVQEQSLGATRVLVLARKAVDLKTRKSVVVVATEQNFNWVDDLKAALATAATEEQYVYVVCQGEELFGAVGLMTCIKNENGGKLARLVFVQDAKAEKFSLTSTLYRQQLEKDLISNVLKNGAWGTFRHLKLETQQATLQVEHAYVNALVKGDLASLKWIEAAQADTAATVDKNLETCTVYYAPINFRDVMLTSGKLAADALPGDLAEQDCVLGLEFAGRDTQGRRVMAMVPAKSLATTCVASKRMMWQIPEKWTMEEASTVPCVYSTVYYALVVRGQMKKGEKILIHAGSGGVGQAAISVALAHGLTVFTTVGSKEKREFLLKRFPKLQERNIGNSRDTSFEQLVLRETKGRGVDLVLNSLSEEKLQASIRCLGLNGRFLEIGKFDLSNNSPLGMSVFLKNTSFHGILLDSVMEGEEEMQNQVVSLVAEGIKTGAVVPLPTSVFNDQQVEQAFRFMASGKHIGKVVIKVRDEEAGKKALQPKPRLINAIPRTYMHPEKSYILVGGLGGFGLELTNWLVTRGARYIVLTSRSGVKTGYQGLMIRRWQERGVKVVIDTSDVTTAAGAKKLLENSNKLALVGGIFNLAAVLRDALIEDQTAKDFKTVADPKVTATKYLDQFSRDICTELDYFICFSSVSCGRGNIGQTNYGLANSAMERICEQRQVSGFPGTAIQWGAIGDTGLVLENLGDNDTVIGGTLPQRMPSCLQTIDLFLQQPHPVVASMVVAEKRKSDQSAGVSLIATIANILGLRDTKNIQDGASLADLGMDSLMSAEIKQTLERNFDIVLSAQEIRQLTFGALKAMDGGADVKPAAAAPAAAAGVPEANITSGGSSRTASPMGDGTQVVFTTSLIPTEAIVQLDTKAPANSKQSPIFFISPIEGFASALEPLAKRLEVPAYGLQYTEAVPSDSLESAAKFFIKQLRTVQPKGPYKLAGYSFGCLLTYVMAGILEETNEVANVIMLDGAPSYVNWYTSSFKQRYTDGTNADNDNQSYGLAYFGIVLANIDYKALVRLLIVIPTWEEKLERFAELMSNEITQPVETIKKSATLFYKKLELADGYQPTLKLKTNVTLVKPTDNSAKLDEDYRLKEVCTKPVEVHTVEGNHRTFLIEDQSLKTIQSILKRLFN

Target ORF information:

RefSeq Version	NM_001144306.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fatty acid synthase 1, transcript variant C (FASN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144306.2

ORF Insert Sequence:

ATGCCCCTAC AACTAAACCT GTTGCAAGCC GTGGACCAGC CGCATCCTGT TGCTTTCAAG 
CCCAACAATT GCAGCACCCA TCAGCCCCAA CATTCACCCA GCACCCAGAA CAACGGAAAG 
AGCGCTCGCG CCAATCGTCG TCGACGCCGC AGACGCAACA AGCAGCAGCA GAATTCGCAG 
CAACCTGACG CCTTCCGTGA TACCGACGCG CAGCTAACAC CGAGCACCTC CTCGTCCACC 
CCGCACTCTG CACCCAACGA TAGACAGGAC CACCTAAACT CCCAATTCAA TCCCGAATCC 
ATCAATATGC CCGCCCGATT CGCCGAGGAA GTCATCACCG CTGAGCCCGC ACAACGTGCC 
GCCCCCCAAT TGGACCTGGG TGGTGGTCAC TATGTGCCCC GTCAGCAGCA CCTCAACGAT 
GAGATCGCCA TCACCGGTTT CTCCGGCCGT CTGCCGGAGA GCTCCACCAT CGAGGAGTTC 
AAGCAGAATC TTTTCGACGG CGTCGACATG GTCAACGATG ATCCCCGCCG TTGGGAGCGT 
GGTCTGTATG GACTTCCCGA CAGGATTGGC AAGCTCAAGG ATAGCGATCT GGAGAACTTT 
GACCAACAGT TCTTCGGTGT GCACCAAAAG CAGGCTGAGT GCATGGACCC ACTGCTGCGC 
ATGCTGCTCG AGCTCACCCA TGAAGCTATT ATTGACGCTG GCCTAAACCC AAGTGATCTG 
CGCGGTTCCC GCACTGGTGT GTACATCGGT GTGTCCAACT CGGAGACTGA GCAGCACTGG 
TGCAGCGATG CTGACCGGGT TAACGGCTAC GGCTTGACAG GATGTGCCCG TGCCATGTTT 
GCCAACCGCA TCTCCTTCAC CTTTGATTTC AAGGGACCCA GCTACAGCAT TGACACCGCT 
TGCTCCAGTT CTCTGTACGC CTTGGAACAG GCTTTCTCCG ATATGCGCGA AGGAAAGGTC 
GACAACGCCC TGGTCGCTGG AGCTGGACTG ATCCTCAAGC CCACCATGTC GCTGCAGTTC 
AAGCGACTGA ATATGTTGAG CCCGGACGGC AGCTGCAAGG CCTTCGATGA GTCTGGCAAT 
GGATACGTCC GTTCCGATGG ATGTGTGGTG CTGCTCCTGC AGCGCACCTC TGCAGCCAGG 
CGTGTGTATG CCTCCATCCT CAATGTGCGC ACCAATACGG ATGGTTTCAA GGAGCAGGGC 
ATCACATACC CTATTGGCAA GATGCAAAAT CGCCTGATCC GCGAGACCTA CGAGGAGATT 
GGTCTTAACC CCGCCGATGT GGTTTACGTG GAGGCACACG GTACCGGAAC CAAGGTGGGC 
GATCCCCAGG AGGTGAACTC TATCACTGAC TTCTTCTGCA AGGACCGTAC GACCCCTCTG 
TTGATCGGAT CGGTCAAGTC CAACATGGGT CACTCGGAAC CCGCTTCCGG TGTCTGCTCT 
GTGGCCAAGA TTCTGATCGC CATGGAGGAG GGCGTCATTC CCGGTAACTT GCACTACAAC 
AAGCCGAACC CAGATCTTTA TGGACTCGTT GATGGACGTC TCAAGGTGGT CGACAGGAAT 
CTGCCTTGGA ACGGTGGCAT CATCGGCCTG AACTCCTTTG GTTTTGGAGG AGCCAACGCT 
CACGTTATCT TAAAGTCGAA CCCCAAGCCC AAGGCTTTGA CCCCCAAGGA TGGAGCACTG 
AAAGTGGTCC TCGCCTCTGG ACGCACCTTT GAGGCTGTGG AGCAGTTGCT CGAGTCCGCC 
TCTACTAATG CCGATGATGA TGAGTACCTG CAACTGATCA ACGAGATCCA CTCAAAGGCT 
ATTCCGAATC ACTTCTTCCG TGGCTACGGA GTGGTGAGCA GCAAGGGCAC CCACCAACGT 
GAGGTGATCG AGTCCAACGA CGATAAACGC CCTATTTGGT ACATCTACTC CGGCATGGGC 
AGCCAGTGGG CTAGCATGGC CAAGGATCTG ATGAAGATCG AGGCCTTCGC CAAGACCATT 
CAGAGGTGTG CCGATGTCCT AAAGCCCGAG GGAGTGGATC TAATCGATGT CCTGACCCGT 
TCCACGGACA AGAGCTTCGA AAACATTCTG AACTCTTTCA TCTCGATTGC TGCCATGCAA 
GTGGCTCTTA CCGATTTGCT CAGCTCGCTG GGCATCCACC CCGACGGAAT TGTGGGACAC 
TCGGTGGGAG AGCTTGGATG CGCCTACGCC GATGGCTGCT TCACCCCGGA GCAGACCGTG 
CTGGCTGCTT ACTGGCGAGG AAAGAGCATT CTGGACACTC AACTGGCCAA GGGCAAGATG 
GCCGCTGTGG GATTGAGCTG GGAAGATGCG CACAGCCGTG TGCCCAGCGA TTGCTTCCCG 
GTGTGCCACA ACAGCGAGGA CAACTGCACC ATCTCTGGCC CGGAGGCTTC CATCGAGGCT 
CTGGTCGCTA AACTGAACGC GGAGGGAGTG TTCGCCAAGG CAGTGAACTC ATCCGGCTAC 
GCTTTCCACA GCAAGTACAT CGCTGAAGCT GGACCCAAAC TACGCAAGAG TCTGGAGAAG 
ATCATTCCCA ACGCTAAGAA CCGTACCGCC CGCTGGATCA GTACCAGCAT CCCAGAATCC 
GCTTGGAACA CACCGGTGGC CAAGCAATCC TCGGCCGCCT ACCACGTGAA CAATCTGCTG 
TCGCCAGTGC TCTTCCACGA GGCCCTTCAG CACGTTCCCA AGAACGCCAT TTCCGTGGAG 
ATTGCTCCCC ACGGCCTGCT CCAGGCTATC CTCAAGCGCG CCCTGGGTCC TGATGCCACC 
AATCTGAGCT TGGTGAAGCG CGGACACGAG AACAACGTTG AGTTCTTCCT CACCAATGTG 
GGCAAACTCT TTGCCGCCGG AGCCCAGCCT CAGGTACTTA CTCTAGTGCG TCCTATCAGC 
TACCCGGTGG GTCGTGGAAC CCCCATGTTG AACTCCAAGG TCGGATGGGA CCACACCCAG 
AAGTGGCTGG TGGCCAAGTT CGGCAAGGAA ACTTCTTCTG GTGAGACCAT CGTGGAAGTC 
GATCTCTCCA AGGAGGATGA TGCTTTCCTG GCTGGACACA CGATCGACGG ACGTATCCTC 
TTCCCGGCCA CTGGCTACAT GACTCTGGCT TGGCAGACAT TCGCTAAGAT GCAGGGAAGT 
GAGTTCCACA AGACCCCGGT GGTAATGGAG AACCTCGTGT TCCACCGTGC CACTATTCTT 
AACAAGAACG CAGTGGTCAA GTTTGGCATT AACTTCTTCG ATGGAACTGG CGCTTTTGAG 
ATTTGTGAGA GCGGTAGCTT GGCGGTTTCT GGAAAAATAA CCATTCCAGA GTCTATTGAC 
AACGAGGAGT TGCCCCTGGA GGAGCAAACT CCCAGCGCTG TTGCCAAGGA GCTAGGCACC 
AACGATGTCT ACAAGGAGCT GCGCCTCCGA GGCTACGACT ATGGTGGAAT TTTCCGCGGC 
ATTGTTCGTT CTGACACCGT GGCCTCCACT GGAAAGCTTC AGTGGGTGGA CAACTGGATT 
AGTTTCATGG ACACCATGTT GCAGTTCAGT ATCCTTAGCA AGAACCTTCG TGAGCTGTAC 
CTGCCCACTC GCATCGAGCG AGCGGTGATT AATCCCGCCA AGCACTTTGA ATTGCTGAGC 
GCCCTCACCA AGGAGGAACA GGTTGAGACT GGACTACCTG TGCAGTGGTA CAGCGACATT 
AACGTGATCA AGAGTGCGGG CGTGGAGCTG CGCGGTCTAA AGGCCAATCT CGCCCAGCGT 
CGTCCTGGCA CACAGGCACC TCCCACTCTG GAGCGCTACC AGTTTGTGCC CAACATCAAC 
ACCACGGATC TAAATGAAAA CTCTGAGAAG GCTCGCCTGC ACGCCTTGGA TGTGGCCATC 
CAGGTGATCA TCGAGAACTC CAGCGGAGCC GTCAAGCTAA AGGGCGTGGA ATTGGCCAAT 
GGACGCAACC CCGACGTTTT GGTTGCCAAC CGCCTGCTGC AGATTATCGA GGGTGAGCCC 
GTGCTTACTG GTGATGTGGC TGTGGTCACC TCGAACAACA ATGAAGAGAC CATAACCGCC 
GCCCTAGGAG ATTCAGGAGT TCGAGTGGTG TCCAAGGACG TGCTGAAGGA ACCGGTAGAG 
CAGAACTGTC ACTTCGTCTT TGGCATCGAT GTCCTTTCCC GACCGGACAC CAAGACCCTG 
GAGAACTCCA TTGCCAGCAT TCGCGAGAAC GGTTTCCTGA TCCTTGAGGA GACGCTGCCC 
ACTTACACCA AGACAGGCCG TGCCCTGCTG ACCAAATTTG GATTCGTTGC CGTTCAGGAA 
CAGAGTCTGG GAGCCACCCG TGTCCTGGTC CTCGCCCGCA AGGCTGTTGA TCTGAAAACT 
CGCAAGTCCG TTGTGGTTGT GGCCACCGAG CAGAACTTTA ACTGGGTGGA TGACTTGAAG 
GCCGCTCTGG CTACTGCTGC TACGGAGGAG CAGTATGTGT ACGTGGTTTG CCAGGGAGAG 
GAGCTGTTTG GAGCTGTGGG TCTGATGACG TGCATCAAGA ACGAGAATGG CGGCAAACTG 
GCTCGCCTGG TCTTCGTGCA AGATGCCAAG GCTGAGAAGT TCTCCCTTAC ATCGACCCTG 
TACCGCCAGC AGCTGGAGAA GGACCTCATC TCGAACGTGC TGAAGAACGG CGCTTGGGGC 
ACTTTCCGTC ACCTGAAACT GGAGACCCAG CAGGCCACTC TCCAAGTGGA GCATGCCTAT 
GTAAACGCTC TGGTCAAGGG TGATCTTGCT TCCCTCAAGT GGATCGAGGC TGCCCAGGCG 
GATACCGCTG CAACTGTCGA CAAGAACCTG GAAACTTGCA CCGTCTACTA TGCGCCCATC 
AACTTCCGTG ACGTTATGTT GACCTCTGGT AAGCTGGCCG CGGATGCCTT GCCTGGAGAT 
CTGGCTGAGC AAGATTGTGT GCTGGGTCTG GAGTTCGCTG GTCGCGATAC CCAGGGTCGT 
CGTGTAATGG CCATGGTGCC GGCTAAATCA CTTGCTACCA CCTGCGTGGC AAGCAAGCGC 
ATGATGTGGC AAATCCCTGA GAAGTGGACC ATGGAAGAGG CTTCGACCGT TCCTTGCGTG 
TACTCCACCG TCTACTACGC TCTGGTTGTG CGAGGACAAA TGAAGAAGGG TGAGAAGATC 
CTCATTCACG CTGGCTCCGG AGGAGTTGGT CAGGCGGCCA TCTCTGTGGC TCTGGCCCAT 
GGCTTGACCG TGTTCACCAC GGTGGGCAGC AAGGAAAAGC GCGAGTTCCT GCTGAAGCGA 
TTCCCCAAAC TGCAGGAGCG TAACATCGGC AACTCCCGAG ACACCTCCTT CGAGCAGTTG 
GTCCTGCGCG AGACCAAGGG ACGCGGAGTC GATCTGGTGC TGAACTCGTT ATCTGAAGAG 
AAACTGCAGG CCTCGATCCG CTGTTTGGGT CTGAATGGAC GCTTCCTGGA GATTGGCAAG 
TTCGATTTGA GCAACAACAG CCCACTTGGA ATGTCCGTTT TCCTCAAGAA CACCTCCTTC 
CACGGCATTT TGCTTGACAG TGTGATGGAG GGCGAGGAGG AGATGCAAAA CCAGGTTGTC 
TCCCTGGTCG CTGAGGGCAT CAAGACCGGA GCCGTTGTAC CCCTTCCGAC CTCGGTGTTT 
AACGACCAGC AGGTGGAGCA GGCCTTCCGT TTCATGGCCT CTGGCAAGCA CATCGGCAAG 
GTAGTGATCA AGGTGCGTGA TGAGGAGGCA GGCAAGAAGG CGCTGCAGCC CAAGCCGCGC 
CTGATCAACG CCATTCCACG CACATACATG CACCCGGAGA AGAGTTACAT CCTGGTTGGA 
GGTCTTGGCG GATTCGGTTT GGAGCTGACC AACTGGTTGG TGACGCGTGG AGCCCGCTAC 
ATTGTGCTGA CCTCTCGTTC CGGAGTTAAG ACCGGCTACC AGGGTCTGAT GATCCGTCGC 
TGGCAGGAAC GTGGCGTAAA GGTGGTGATT GACACCAGTG ATGTGACCAC CGCCGCTGGA 
GCTAAAAAGC TGCTGGAGAA TAGTAACAAG CTGGCTTTGG TCGGAGGCAT CTTCAACCTG 
GCGGCTGTTC TCCGCGATGC TCTGATCGAG GATCAGACCG CCAAGGACTT CAAGACAGTG 
GCTGATCCCA AGGTGACGGC CACCAAGTAC CTGGATCAGT TCTCTCGCGA TATCTGCACT 
GAGCTGGACT ACTTCATCTG CTTCTCCAGT GTTTCCTGCG GTCGTGGTAA CATTGGTCAG 
ACCAACTACG GATTGGCCAA CTCTGCAATG GAGCGAATTT GCGAGCAGCG CCAGGTGAGC 
GGATTCCCAG GCACCGCCAT CCAATGGGGA GCCATCGGAG ACACCGGTCT GGTGCTGGAG 
AACTTGGGTG ACAATGACAC CGTGATTGGA GGAACCCTGC CCCAGAGGAT GCCCTCTTGC 
CTGCAGACCA TCGACCTGTT CCTGCAACAG CCCCATCCGG TTGTTGCCTC CATGGTTGTG 
GCCGAGAAGC GCAAGTCGGA TCAGTCGGCT GGAGTCAGCC TAATTGCCAC CATTGCCAAC 
ATCCTTGGTC TCCGGGACAC CAAGAATATC CAGGACGGAG CATCGCTTGC TGATCTCGGA 
ATGGACTCCC TGATGAGCGC TGAGATCAAA CAGACTTTGG AGCGAAACTT TGACATTGTT 
CTGTCCGCCC AGGAGATCCG TCAGCTCACC TTTGGAGCCC TCAAGGCCAT GGATGGTGGT 
GCTGATGTCA AGCCAGCCGC AGCTGCTCCC GCAGCCGCTG CTGGAGTCCC AGAGGCAAAC 
ATTACCTCTG GTGGATCCTC GCGCACAGCT AGTCCCATGG GCGATGGAAC GCAGGTGGTG 
TTCACCACGT CCCTCATCCC CACCGAGGCA ATTGTACAAC TGGACACAAA GGCACCTGCA 
AACAGCAAAC AGAGCCCCAT CTTCTTCATT TCCCCGATCG AGGGGTTCGC CTCCGCACTG 
GAGCCGCTGG CCAAGCGATT GGAGGTGCCC GCCTATGGAC TGCAGTACAC GGAAGCCGTG 
CCCTCCGATT CTCTGGAATC AGCAGCCAAA TTCTTCATCA AGCAGCTGCG CACCGTCCAG 
CCAAAGGGTC CCTACAAACT AGCCGGATAC TCCTTCGGTT GCCTGCTCAC CTACGTGATG 
GCCGGTATTC TGGAAGAGAC CAACGAGGTG GCCAATGTGA TCATGCTGGA CGGAGCCCCA 
AGCTACGTCA ACTGGTACAC GAGCAGCTTC AAGCAGCGTT ACACTGACGG CACCAACGCC 
GATAACGACA ACCAGAGCTA CGGCTTGGCC TACTTTGGAA TCGTGTTGGC CAACATTGAC 
TACAAAGCGC TGGTGCGTCT GCTAATAGTG ATCCCCACAT GGGAGGAGAA ACTCGAGAGG 
TTCGCTGAGC TGATGAGCAA TGAGATCACC CAGCCCGTGG AAACGATCAA GAAATCCGCC 
ACTCTGTTCT ACAAGAAACT GGAGCTGGCC GATGGCTACC AGCCCACTCT GAAGCTCAAG 
ACGAATGTGA CCTTGGTCAA GCCCACAGAC AACTCTGCCA AGCTGGACGA GGACTATCGC 
CTTAAGGAGG TCTGCACAAA GCCCGTGGAA GTACACACTG TTGAGGGCAA CCACCGCACC 
TTCCTCATCG AGGATCAGTC CCTGAAGACC ATCCAGAGCA TACTGAAGCG TCTGTTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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FASN1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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