Pbp95 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pbp95
Entrez Gene ID	40949
Full Name	PSEA-binding protein 95kD
Synonyms	BEST:LD28511,CG2702,DmPBP,DmPBP95,DmSNAP190,DmSNAPc,Dmel\CG2702,anon-WO0118547.359
General protein information	Preferred Names PSEA-binding protein 95kD Names CG2702 gene product from transcript CG2702-RA CG2702-PA PSEA-binding protein Pbp95-PA drosophila melanogaster PSE-binding protein 95-kDa subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84E8-84E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_141503.4	NP_649760.1	PSEA-binding protein 95kD

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001156	XP_001689386.2
Drosophila melanogaster (fruit fly)	Pbp95	NP_649760.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of the study are consistent with a model in which the specific DNA sequences of the U1 and U6 Promoter differentially alter the conformation of DmSNAPc, leading to the subsequent recruitment of different RNA polymerases to the U1 and U6 gene promoters.
Title: The Myb domain of the largest subunit of SNAPc adopts different architectural configurations on U1 and U6 snRNA gene promoter sequences.

The results reveal the topological arrangement of the 4.5 SNAP190 Myb repeats relative to the DNA and to each other when SNAP190 is bound to a U1 promoter as a subunit of SNAPc.
Title: Architectural arrangement of the small nuclear RNA (snRNA)-activating protein complex 190 subunit (SNAP190) on U1 snRNA gene promoter DNA.

The results indicated that the C-terminal domain of DmSNAP190 is essential for DmSNAPc to bind efficiently to the PSEA, even though it is not required for assembly with either DmSNAP43 or DmSNAP50 .
Title: A map of Drosophila melanogaster small nuclear RNA-activating protein complex (DmSNAPc) domains involved in subunit assembly and DNA binding.

Results support a model in which conformational differences in proximal sequence element binding protein (DmPBP)-DNA complexes lead to selective RNA polymerase recruitment to U1 and U6 promoters.
Title: Architectural arrangement of cloned proximal sequence element-binding protein subunits on Drosophila U1 and U6 snRNA gene promoters.

The following Pbp95 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pbp95 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07981	NM_141503.4 Latest version!	Drosophila melanogaster PSEA-binding protein 95kD (Pbp95), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pbp95 ( NM_141503.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649760.1 Drosophila melanogaster PSEA-binding protein 95kD (Pbp95), mRNA.

Price & Availability↑

CloneID	OFa07981
Clone ID Related Accession (Same CDS sequence)	NM_141503.4
Accession Version	NM_141503.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PSEA-binding protein 95kD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141503.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGATG CCGATGATCT GCGCATAAAC CAGCAGCAGC TTATGGACGA GATTTCGGCC 
CTCTTGCAGA ACACAGCGAA GCCGAGCGCC GCCAACGCAC TGCAGCTGAA TCAGGAGCTC 
CAGCGCCGGC TCATGCAAGT CCGGACCAAA ATTCTGGCCA TGTTACAAGT AGTAAGGGCC 
CGCTTCTCTC GGAACGAGGA CATCCTGGTG CGCCGGCTGC GACCTCGTTC CCATTTCGGC 
CCGAATTCCC TCAATCTTAG CGGGGCGATC CTGCGGAAGG GCACGTTTCG CTTCAAGGGC 
AACCTGTTTT TCCGAGACAT CGATGGTCGA AGCTGTCCTA ACAACGAGGA TTACGAGGCG 
CGGTGCCACA CGGAGATGTT TCCCACCGAC TTTGACATGC ACTCCAGACA TGTATGGACA 
TTGCTAGACA AAAAGAATGT GATAATGGGT ATAAAACAAC AGTTACTGGA GCATAGGGCC 
CACAGTACGA ATGCGCTGCC CAGCGGAAGC CTGAAGCGCA AGCCAATCGA GCGACACTTA 
AGTACACTGG TAAGTCTGCT GGCAACTGCG GATAGTAGCT TCAGCATCGA CTGGAACCAG 
ATCAGCACCC TGGACTTGGA ATATCGGCAC TCACCATATA GCTGTGAGGC AATGTGGAGG 
GTCTACTTAA CACCAGATCT CAGGCGAGAC GATTGGTCAC CCGAGGAGGA CGAAACTTTA 
CTGGCGGTGG CCACTGCGAA CAGGATGCAG AACTGGGAGT TGATAGCCGC GTCGCTAGAC 
CGACGTTCCG ACTATCAGTG CTTCGTACGC TTTCATACCG CCCTACGTTT TCTGCTGGAG 
CCAAAGAACA GCCACCGCTG GAGCGAAGAG GACAACGATA AACTAAGGGC GATCGTTGAT 
AGGAATACGG CAAACAGTGT AATAAACTGG AAAAAAGTCG TGGAATACTT CCCGGACAAG 
TCCAAATCAA CGCTGATTGG TCGTTACTAC TACGTGTTGC ATCCTAGCAT CAGTCACGAA 
CCCTTCACTA CCAAGGAGGA CATGATGCTG TTCGCCGCCG TTGAAGAGTA CAATGGAAAG 
TTTCACTGTT TTCCGCGTTC TCTTTTCCCA AACCGGTCGT TAACCCAACT CCGAACTCGC 
TACCACAATG TACTGGCACA ACGCAACAAG ACCGACTCCT GGTCCGTTCA GGACGACACA 
CGGCTGATGA GCTTCGTAAC GCAATACGGG GCTTCCCAGT GGCTTAACTG TGCCACGTTC 
TTGGGCAATC ACACGCGGAC CAGCTGTCGA ACCCGCTTCC TAGTCATCAA GAAATTCCTT 
GAACAAAATC CCAATGCAAA AGTGGAAGAC CTGCCACGCC GCCGATCGAA GAAGGTGTCC 
TTGGTTAACT CTGATAACTG GGCGCAGCGT TTGCAAGAGT GGCAAGAGGA CCCAGAGTCA 
TTAGTTAATG ATAACCCCCC GAAAGGAACT CGGGTGCGCG GTCCCAAATC GAAAAAGGCT 
AGGATCGAGC GACAGGCAGA GAGTTTCTCA AGACTTTCGA AAGTTGATAT AGAGTTCTGC 
AACTTCTTTA AGTTTAGTTA TAATCTGACG CTGAGCACTC CAAAAACTTT TCCAGTTCCA 
AAAGACGTTT ATAACCTTGC CTACGTGATC AGAGCTCTTG CATATAAGCC ACCAATTCGA 
CCCTCGCTCT TACAAAACAT ATTTATGCCA AATGATGTGC TTAAGTGCTA CAACAGCATG 
ATCCGAAACC TTCCGGACGA GGAAGGTGAT ATGAAGAGCC CACTTTTGCC ACCCAACTGG 
TCAACTATGA TGGGGTTTCG AGCCTTATGC ATTCTTTCCG GAGATTGTCG CAAGGATACG 
GAAACACGTA GCTTCGAATA CAATGAATCT TTACCACCCA TCCAGCTTTT CCGCAAGCGG 
TTGCAAGCTC TTTTCTATCG TACCACTCTT CTCAGCCGCT TGGAATCACA ACTCTTTACG 
GACTTACCAT CAGCATTAGT TTCTTTACCA CGCCCCAAAC ACGATTATGC AAAAATGGGT 
ACACATGTTG AATTAATCGA TCCAGAGCCG GCACCACAGA ATGATCTAAA ATCCGAGCCG 
CTTAGCGAGA ATGAAGTGAT TAATACAGTC AAGCAGGAAT TGGAAATGGA ATATACAGTT 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649760.1
CDS	48..2213
Translation	MDDADDLRINQQQLMDEISALLQNTAKPSAANALQLNQELQRRLMQVRTKILAMLQVVRARFSRNEDILVRRLRPRSHFGPNSLNLSGAILRKGTFRFKGNLFFRDIDGRSCPNNEDYEARCHTEMFPTDFDMHSRHVWTLLDKKNVIMGIKQQLLEHRAHSTNALPSGSLKRKPIERHLSTLVSLLATADSSFSIDWNQISTLDLEYRHSPYSCEAMWRVYLTPDLRRDDWSPEEDETLLAVATANRMQNWELIAASLDRRSDYQCFVRFHTALRFLLEPKNSHRWSEEDNDKLRAIVDRNTANSVINWKKVVEYFPDKSKSTLIGRYYYVLHPSISHEPFTTKEDMMLFAAVEEYNGKFHCFPRSLFPNRSLTQLRTRYHNVLAQRNKTDSWSVQDDTRLMSFVTQYGASQWLNCATFLGNHTRTSCRTRFLVIKKFLEQNPNAKVEDLPRRRSKKVSLVNSDNWAQRLQEWQEDPESLVNDNPPKGTRVRGPKSKKARIERQAESFSRLSKVDIEFCNFFKFSYNLTLSTPKTFPVPKDVYNLAYVIRALAYKPPIRPSLLQNIFMPNDVLKCYNSMIRNLPDEEGDMKSPLLPPNWSTMMGFRALCILSGDCRKDTETRSFEYNESLPPIQLFRKRLQALFYRTTLLSRLESQLFTDLPSALVSLPRPKHDYAKMGTHVELIDPEPAPQNDLKSEPLSENEVINTVKQELEMEYTVP

Target ORF information:

RefSeq Version	NM_141503.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PSEA-binding protein 95kD (Pbp95), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141503.4

ORF Insert Sequence:

ATGGACGATG CCGATGATCT GCGCATAAAC CAGCAGCAGC TTATGGACGA GATTTCGGCC 
CTCTTGCAGA ACACAGCGAA GCCGAGCGCC GCCAACGCAC TGCAGCTGAA TCAGGAGCTC 
CAGCGCCGGC TCATGCAAGT CCGGACCAAA ATTCTGGCCA TGTTACAAGT AGTAAGGGCC 
CGCTTCTCTC GGAACGAGGA CATCCTGGTG CGCCGGCTGC GACCTCGTTC CCATTTCGGC 
CCGAATTCCC TCAATCTTAG CGGGGCGATC CTGCGGAAGG GCACGTTTCG CTTCAAGGGC 
AACCTGTTTT TCCGAGACAT CGATGGTCGA AGCTGTCCTA ACAACGAGGA TTACGAGGCG 
CGGTGCCACA CGGAGATGTT TCCCACCGAC TTTGACATGC ACTCCAGACA TGTATGGACA 
TTGCTAGACA AAAAGAATGT GATAATGGGT ATAAAACAAC AGTTACTGGA GCATAGGGCC 
CACAGTACGA ATGCGCTGCC CAGCGGAAGC CTGAAGCGCA AGCCAATCGA GCGACACTTA 
AGTACACTGG TAAGTCTGCT GGCAACTGCG GATAGTAGCT TCAGCATCGA CTGGAACCAG 
ATCAGCACCC TGGACTTGGA ATATCGGCAC TCACCATATA GCTGTGAGGC AATGTGGAGG 
GTCTACTTAA CACCAGATCT CAGGCGAGAC GATTGGTCAC CCGAGGAGGA CGAAACTTTA 
CTGGCGGTGG CCACTGCGAA CAGGATGCAG AACTGGGAGT TGATAGCCGC GTCGCTAGAC 
CGACGTTCCG ACTATCAGTG CTTCGTACGC TTTCATACCG CCCTACGTTT TCTGCTGGAG 
CCAAAGAACA GCCACCGCTG GAGCGAAGAG GACAACGATA AACTAAGGGC GATCGTTGAT 
AGGAATACGG CAAACAGTGT AATAAACTGG AAAAAAGTCG TGGAATACTT CCCGGACAAG 
TCCAAATCAA CGCTGATTGG TCGTTACTAC TACGTGTTGC ATCCTAGCAT CAGTCACGAA 
CCCTTCACTA CCAAGGAGGA CATGATGCTG TTCGCCGCCG TTGAAGAGTA CAATGGAAAG 
TTTCACTGTT TTCCGCGTTC TCTTTTCCCA AACCGGTCGT TAACCCAACT CCGAACTCGC 
TACCACAATG TACTGGCACA ACGCAACAAG ACCGACTCCT GGTCCGTTCA GGACGACACA 
CGGCTGATGA GCTTCGTAAC GCAATACGGG GCTTCCCAGT GGCTTAACTG TGCCACGTTC 
TTGGGCAATC ACACGCGGAC CAGCTGTCGA ACCCGCTTCC TAGTCATCAA GAAATTCCTT 
GAACAAAATC CCAATGCAAA AGTGGAAGAC CTGCCACGCC GCCGATCGAA GAAGGTGTCC 
TTGGTTAACT CTGATAACTG GGCGCAGCGT TTGCAAGAGT GGCAAGAGGA CCCAGAGTCA 
TTAGTTAATG ATAACCCCCC GAAAGGAACT CGGGTGCGCG GTCCCAAATC GAAAAAGGCT 
AGGATCGAGC GACAGGCAGA GAGTTTCTCA AGACTTTCGA AAGTTGATAT AGAGTTCTGC 
AACTTCTTTA AGTTTAGTTA TAATCTGACG CTGAGCACTC CAAAAACTTT TCCAGTTCCA 
AAAGACGTTT ATAACCTTGC CTACGTGATC AGAGCTCTTG CATATAAGCC ACCAATTCGA 
CCCTCGCTCT TACAAAACAT ATTTATGCCA AATGATGTGC TTAAGTGCTA CAACAGCATG 
ATCCGAAACC TTCCGGACGA GGAAGGTGAT ATGAAGAGCC CACTTTTGCC ACCCAACTGG 
TCAACTATGA TGGGGTTTCG AGCCTTATGC ATTCTTTCCG GAGATTGTCG CAAGGATACG 
GAAACACGTA GCTTCGAATA CAATGAATCT TTACCACCCA TCCAGCTTTT CCGCAAGCGG 
TTGCAAGCTC TTTTCTATCG TACCACTCTT CTCAGCCGCT TGGAATCACA ACTCTTTACG 
GACTTACCAT CAGCATTAGT TTCTTTACCA CGCCCCAAAC ACGATTATGC AAAAATGGGT 
ACACATGTTG AATTAATCGA TCCAGAGCCG GCACCACAGA ATGATCTAAA ATCCGAGCCG 
CTTAGCGAGA ATGAAGTGAT TAATACAGTC AAGCAGGAAT TGGAAATGGA ATATACAGTT 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pbp95 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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