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neur cDNA ORF clone, Drosophila melanogaster(fruit fly)

Summary

Gene Symbol neur
Entrez Gene ID 41085
Full Name neuralized
Synonyms 0328/12,0971/15,1293/09,1315/05,BEST:LD21494,CG11988,Dmel\CG11988,Neur,l(3)j6B12,l(3)j9B8,l(3)j9B9,l(3)j9C8,l(3)neo37,neu
General protein information
Preferred Names

neuralized

Names

CG11988 gene product from transcript CG11988-RD
CG11988-PA
CG11988-PB
CG11988-PC
CG11988-PD
CG11988-PE
neur-PA
neur-PB
neur-PC
neur-PD
neur-PE
neuralised
Gene Type protein-coding
Organism Drosophila melanogaster(fruit fly)
Genome

3R

85C2-85C3|3-48.5 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_169255.3 NP_731309.1 neuralized, isoform D
NM_169256.3 NP_731310.1 neuralized, isoform C
NM_001170092.3 NP_001163563.1 neuralized, isoform E
NM_057304.5 NP_476652.1 neuralized, isoform A
NM_169257.3 NP_731311.1 neuralized, isoform B

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) NEURL1B XP_003311007.1
Mus musculus (house mouse) Neurl1b NP_001075125.1
Rattus norvegicus (Norway rat) Neurl1b NP_001136124.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001999 XP_003435991.1
Bos taurus (cattle) NEURL1B XP_002696317.2
Homo sapiens (human) NEURL1B NP_001136123.1
NEURL1B XP_002804683.1
Canis lupus familiaris (dog) NEURL1B XP_005619504.1
Gallus gallus (chicken) NEURL1B XP_425198.2
Drosophila melanogaster (fruit fly) neur NP_731311.1
Caenorhabditis elegans (roundworm) F10D7.5 NP_510818.3
Xenopus tropicalis (tropical clawed frog) neurl1b XP_002941593.1

Gene Ontology


Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the NHR2 domain is required for Neur function, and a point mutation in the NHR2 domain (Gly430) abolishes Neur ubiquitination activity and affects ligand internalization
Title: Functional analysis of the NHR2 domain indicates that oligomerization of Neuralized regulates ubiquitination and endocytosis of Delta during Notch signaling.

The ubiquitylation of Delta by both Neuralized and Mindbomb1 was detected, with the two enzymes using distinct docking sites and having different acceptor lysine preferences.
Title: Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Neuralized.

results tie Numb and Notch-signaling through a single player, Neur, thus giving us a more complete picture of the events surrounding asymmetric division of precursor cells
Title: Neuralized mediates asymmetric division of neural precursors by two distinct and sequential events: promoting asymmetric localization of Numb and enhancing activation of Notch-signaling.

inhibition and activation of Notch signaling rely on a conserved Neuralized-binding motif in Bearded proteins and the Notch ligand Delta
Title: Both inhibition and activation of Notch signaling rely on a conserved Neuralized-binding motif in Bearded proteins and the Notch ligand Delta.

the highly conserved ubiquitin ligase Neuralized (Neur) is expressed in the adult Drosophila mushroom body (MB) alpha/beta lobe peripheral neurons and is a limiting factor for the formation of long-term memory
Title: Neuralized is expressed in the alpha/beta lobes of adult Drosophila mushroom bodies and facilitates olfactory long-term memory formation.

The following neur gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the neur cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa04310 NM_169255.3
Latest version!		 Drosophila melanogaster neuralized (neur), transcript variant D, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa04311 NM_169256.3
Latest version!		 Drosophila melanogaster neuralized (neur), transcript variant C, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa04311 NM_001170092.3
Latest version!		 Drosophila melanogaster neuralized (neur), transcript variant E, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa04312 NM_169257.3
Latest version!		 Drosophila melanogaster neuralized (neur), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa04313 NM_057304.5
Latest version!		 Drosophila melanogaster neuralized (neur), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OFa04310
Clone ID Related Accession (Same CDS sequence) NM_169255.3
Accession Version NM_169255.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product neuralized, isoform D
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169255.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGTCAGT CGGCTGGTAA AATAGTTCGC CGCTCACCCT CTTCATGTCC TGGCCCCAAC 
AACCTGCCTC CGCTGCAATT CCACTCCGTA CATGGCGACA ACATCCGAAT TTCAAGGGAC 
GGAACCCTGG CCCGTCGCTT TGAGAGCTTC TGCAGGGCCA TCACCTTTTC AGCGCGTCCG 
GTTCGCATCA ACGAGAGGAT CTGCGTGAAG TTTGCTGAGA TCTCGAACAA CTGGAACGGT 
GGAATTCGCT TTGGATTCAC CAGCAACGAT CCTGTAACGC TGGAGGGAAC GCTGCCCAAG 
TACGCCTGTC CGGATCTGAC CAACAGGCCT GGCTTCTGGG CCAAGGCTCT GCACGAGCAG 
TACTGCGAGA AGGACAACAT ACTTTACTAC TATGTCAATG GAGCTGGCGA TGTGATCTAT 
GGTATAAACA ACGAGGAGAA GGGTGTGATA CTGACCGGCA TCGATACGAG AAGCCTGCTG 
TGGACGGTGA TCGATATCTA TGGCAACTGC ACGGGCATTG AGTTTCTCGA TTCTCGCATT 
TACATGTACC AGCAGCAGCC AGCGGCTATA CCAATGGCCA CTGTGCCCGC CCAGCAACAG 
CAAATGCCCC AGCCAGCTGC CAATGCCTCC TCCGCCCTGA ATTCCCATCA CCCGCATCAG 
CAGTCGAGGA GATCCCTGCC AGGACACACT GCGGCCATCG AGCACGATCT GGAGCGCCAT 
GTGATGCCGT CCCTGCAATC CCTGCACTTG GCAGGAAACG GTGGAAGCGT GGCTAGCGTA 
GAACAGGCGG CCATTGCTCA TGATTTGGCC AATGGACTGC CTCCTCTGCG CTACAATGCC 
AACGGACGAC TGATTCCAGT GCCCTTCCAC AACACCAAGG GCCGGAATGT GCGGCTCTCG 
CAGGATCGCT TTGTGGCTTC TCGTACGGAA TCTGACTTCT GCCAGGGATA TGTGTTCACT 
GCCCGTCCCA TTCGCATTGG TGAGAAGCTG ATTGTGCAGG TCCTGAAAAC GGAACAGATG 
TACGTGGGTG CCTTGGCTTT GGGTCTGACC TCCTGCAATC CGGCCATGTT GCAGCCCAAT 
GATTTGCCCA ATGATTCGGA TTTCCTGCTC GATCGCCCCG AGTACTGGGT GGTGAGCAAG 
GATATTGCAG CTGCTCCTCA GCGCGGCGAT GAGATTGCCT TCTTCGTGGC GCCCAACGGT 
GAAGTGAGCA TCAGCAAGAA CAACGGACCA GCTGTGGTGG TGATGCATGT GGACCAGTCC 
CTCCAGCTGT GGGCCTTCCT GGATGTCTAC GGCTCGACGC AGTCTCTGAG GATGTTCCGC 
CAGCAGTTAC CCAACATGGT TGCGTATCCA TCGCAGCCGC AGGTCAATGT GAATGCCAGC 
AGCTCGTCCG CCTGCAACGC TGCCTCCACG TCCCGCATGC TGCCCATGAC CGAGTCGATG 
AGCAGTCTGA ATGCCGGAGC CACTGCCAAG CTGTTGCATC ATCCCTCGCA GCTGAGCGTA 
GCCCAGAGTA CGAGCACTTT GGCCTCAGCT GGCGGAGTCA ATGGCAGCCG CATGATCAGC 
ATGCCCTCCA ACGGAGATAT TCTCCAGATC CAGCCGAATG GTGGTGGCAC CGTTCTGGTG 
GTCAACCTGC CACCTGCTAG CAGCTCGCAC GATATAAACG GCCAACTCGC GGCCAGGCCA 
ACGGCCACGG TAACCTCCAG CGGTGTTCTG GCCGGAGCCT GCTCCAGCGG CACCCTTATC 
AGCACCACCA GCAGCCAGTA CATCGAGCCC ATTGCCAATA GCACAAATAA TGCGGCCAAC 
AAGTGGAAGG ACAGCCTGAG CGACCAGCAG AGCACCGATT CGAGTGCCGA ATGCACCATC 
TGCTACGAGA ATCCCATCGA CTCGGTGCTG TACATGTGCG GACACATGTG CATGTGCTAC 
GACTGCGCCA TCGAGCAGTG GCGCGGAGTG GGTGGTGGCC AGTGCCCACT GTGCCGGGCG 
GTGATCCGGG ACGTCATCCG CACCTACACC ACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_731309.1
CDS293..2308
Translation

Target ORF information:

RefSeq Version NM_169255.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster neuralized (neur), transcript variant D, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_169255.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGTCAGT CGGCTGGTAA AATAGTTCGC CGCTCACCCT CTTCATGTCC TGGCCCCAAC 
AACCTGCCTC CGCTGCAATT CCACTCCGTA CATGGCGACA ACATCCGAAT TTCAAGGGAC 
GGAACCCTGG CCCGTCGCTT TGAGAGCTTC TGCAGGGCCA TCACCTTTTC AGCGCGTCCG 
GTTCGCATCA ACGAGAGGAT CTGCGTGAAG TTTGCTGAGA TCTCGAACAA CTGGAACGGT 
GGAATTCGCT TTGGATTCAC CAGCAACGAT CCTGTAACGC TGGAGGGAAC GCTGCCCAAG 
TACGCCTGTC CGGATCTGAC CAACAGGCCT GGCTTCTGGG CCAAGGCTCT GCACGAGCAG 
TACTGCGAGA AGGACAACAT ACTTTACTAC TATGTCAATG GAGCTGGCGA TGTGATCTAT 
GGTATAAACA ACGAGGAGAA GGGTGTGATA CTGACCGGCA TCGATACGAG AAGCCTGCTG 
TGGACGGTGA TCGATATCTA TGGCAACTGC ACGGGCATTG AGTTTCTCGA TTCTCGCATT 
TACATGTACC AGCAGCAGCC AGCGGCTATA CCAATGGCCA CTGTGCCCGC CCAGCAACAG 
CAAATGCCCC AGCCAGCTGC CAATGCCTCC TCCGCCCTGA ATTCCCATCA CCCGCATCAG 
CAGTCGAGGA GATCCCTGCC AGGACACACT GCGGCCATCG AGCACGATCT GGAGCGCCAT 
GTGATGCCGT CCCTGCAATC CCTGCACTTG GCAGGAAACG GTGGAAGCGT GGCTAGCGTA 
GAACAGGCGG CCATTGCTCA TGATTTGGCC AATGGACTGC CTCCTCTGCG CTACAATGCC 
AACGGACGAC TGATTCCAGT GCCCTTCCAC AACACCAAGG GCCGGAATGT GCGGCTCTCG 
CAGGATCGCT TTGTGGCTTC TCGTACGGAA TCTGACTTCT GCCAGGGATA TGTGTTCACT 
GCCCGTCCCA TTCGCATTGG TGAGAAGCTG ATTGTGCAGG TCCTGAAAAC GGAACAGATG 
TACGTGGGTG CCTTGGCTTT GGGTCTGACC TCCTGCAATC CGGCCATGTT GCAGCCCAAT 
GATTTGCCCA ATGATTCGGA TTTCCTGCTC GATCGCCCCG AGTACTGGGT GGTGAGCAAG 
GATATTGCAG CTGCTCCTCA GCGCGGCGAT GAGATTGCCT TCTTCGTGGC GCCCAACGGT 
GAAGTGAGCA TCAGCAAGAA CAACGGACCA GCTGTGGTGG TGATGCATGT GGACCAGTCC 
CTCCAGCTGT GGGCCTTCCT GGATGTCTAC GGCTCGACGC AGTCTCTGAG GATGTTCCGC 
CAGCAGTTAC CCAACATGGT TGCGTATCCA TCGCAGCCGC AGGTCAATGT GAATGCCAGC 
AGCTCGTCCG CCTGCAACGC TGCCTCCACG TCCCGCATGC TGCCCATGAC CGAGTCGATG 
AGCAGTCTGA ATGCCGGAGC CACTGCCAAG CTGTTGCATC ATCCCTCGCA GCTGAGCGTA 
GCCCAGAGTA CGAGCACTTT GGCCTCAGCT GGCGGAGTCA ATGGCAGCCG CATGATCAGC 
ATGCCCTCCA ACGGAGATAT TCTCCAGATC CAGCCGAATG GTGGTGGCAC CGTTCTGGTG 
GTCAACCTGC CACCTGCTAG CAGCTCGCAC GATATAAACG GCCAACTCGC GGCCAGGCCA 
ACGGCCACGG TAACCTCCAG CGGTGTTCTG GCCGGAGCCT GCTCCAGCGG CACCCTTATC 
AGCACCACCA GCAGCCAGTA CATCGAGCCC ATTGCCAATA GCACAAATAA TGCGGCCAAC 
AAGTGGAAGG ACAGCCTGAG CGACCAGCAG AGCACCGATT CGAGTGCCGA ATGCACCATC 
TGCTACGAGA ATCCCATCGA CTCGGTGCTG TACATGTGCG GACACATGTG CATGTGCTAC 
GACTGCGCCA TCGAGCAGTG GCGCGGAGTG GGTGGTGGCC AGTGCCCACT GTGCCGGGCG 
GTGATCCGGG ACGTCATCCG CACCTACACC ACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa04311
Clone ID Related Accession (Same CDS sequence) NM_169256.3 , NM_001170092.3
Accession Version NM_169256.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product neuralized, isoform C
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169256.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGTCAGT CGGCTGGTAA AATAGTTCGC CGCTCACCCT CTTCATGTCC TGGCCCCAAC 
AACCTGCCTC CGCTGCAATT CCACTCCGTA CATGGCGACA ACATCCGAAT TTCAAGGGAC 
GGAACCCTGG CCCGTCGCTT TGAGAGCTTC TGCAGGGCCA TCACCTTTTC AGCGCGTCCG 
GTTCGCATCA ACGAGAGGAT CTGCGTGAAG TTTGCTGAGA TCTCGAACAA CTGGAACGGT 
GGAATTCGCT TTGGATTCAC CAGCAACGAT CCTGTAACGC TGGAGGGAAC GCTGCCCAAG 
TACGCCTGTC CGGATCTGAC CAACAGGCCT GGCTTCTGGG CCAAGGCTCT GCACGAGCAG 
TACTGCGAGA AGGACAACAT ACTTTACTAC TATGTCAATG GAGCTGGCGA TGTGATCTAT 
GGTATAAACA ACGAGGAGAA GGGTGTGATA CTGACCGGCA TCGATACGAG AAGCCTGCTG 
TGGACGGTGA TCGATATCTA TGGCAACTGC ACGGGCATTG AGTTTCTCGA TTCTCGCATT 
TACATGTACC AGCAGCAGCC AGCGGCTATA CCAATGGCCA CTGTGCCCGC CCAGCAACAG 
CAAATGCCCC AGCCAGCTGC CAATGCCTCC TCCGCCCTGA ATTCCCATCA CCCGCATCAG 
CAGTCGAGGA GATCCCTGCC AGGACACACT GCGGCCATCG AGCACGATCT GGAGCGCCAT 
GTGATGCCGT CCCTGCAATC CCTGCACTTG GCAGGAAACG GTGGAAGCGT GGCTAGCGTA 
GAACAGGCGG CCATTGCTCA TGATTTGGCC AATGGACTGC CTCCTCTGCG CTACAATGCC 
AACGGACGAC TGATTCCAGT GCCCTTCCAC AACACCAAGG GCCGGAATGT GCGGCTCTCG 
CAGGATCGCT TTGTGGCTTC TCGTACGGAA TCTGACTTCT GCCAGGGATA TGTGTTCACT 
GCCCGTCCCA TTCGCATTGG TGAGAAGCTG ATTGTGCAGG TCCTGAAAAC GGAACAGATG 
TACGTGGGTG CCTTGGCTTT GGGTCTGACC TCCTGCAATC CGGCCATGTT GCAGCCCAAT 
GATTTGCCCA ATGATTCGGA TTTCCTGCTC GATCGCCCCG AGTACTGGGT GGTGAGCAAG 
GATATTGCAG CTGCTCCTCA GCGCGGCGAT GAGATTGCCT TCTTCGTGGC GCCCAACGGT 
GAAGTGAGCA TCAGCAAGAA CAACGGACCA GCTGTGGTGG TGATGCATGT GGACCAGTCC 
CTCCAGCTGT GGGCCTTCCT GGATGTCTAC GGCTCGACGC AGTCTCTGAG GATGTTCCGC 
CAGCAGTTAC CCAACATGGT TGCGTATCCA TCGCAGCCGC AGGTCAATGT GAATGCCAGC 
AGCTCGTCCG CCTGCAACGC TGCCTCCACG TCCCGCATGC TGCCCATGAC CGAGTCGATG 
AGCAGTCTGA ATGCCGGAGC CACTGCCAAG CTGTTGCATC ATCCCTCGCA GCTGAGCGTA 
GCCCAGAGTA CGAGCACTTT GGCCTCAGCT GGCGGAGTCA ATGGCAGCCG CATGATCAGC 
ATGCCCTCCA ACGGAGATAT TCTCCAGATC CAGCCGAATG GTGGTGGCAC CGTTCTGGTG 
GTCAACCTGC CACCTGCTAG CAGCTCGCAC GATATAAACG GCCAACTCGC GGCCAGGCCA 
ACGGCCACGG TAACCTCCAG CGGTGTTCTG GCCGGAGCCT GCTCCAGCGG CACCCTTATC 
AGCACCACCA GCAGCCAGTA CATCGAGCAG CCCATTGCCA ATAGCACAAA TAATGCGGCC 
AACAAGTGGA AGGACAGCCT GAGCGACCAG CAGAGCACCG ATTCGAGTGC CGAATGCACC 
ATCTGCTACG AGAATCCCAT CGACTCGGTG CTGTACATGT GCGGACACAT GTGCATGTGC 
TACGACTGCG CCATCGAGCA GTGGCGCGGA GTGGGTGGTG GCCAGTGCCC ACTGTGCCGG 
GCGGTGATCC GGGACGTCAT CCGCACCTAC ACCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_731310.1
CDS293..2311
Translation

Target ORF information:

RefSeq Version NM_169256.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster neuralized (neur), transcript variant C, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_169256.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGTCAGT CGGCTGGTAA AATAGTTCGC CGCTCACCCT CTTCATGTCC TGGCCCCAAC 
AACCTGCCTC CGCTGCAATT CCACTCCGTA CATGGCGACA ACATCCGAAT TTCAAGGGAC 
GGAACCCTGG CCCGTCGCTT TGAGAGCTTC TGCAGGGCCA TCACCTTTTC AGCGCGTCCG 
GTTCGCATCA ACGAGAGGAT CTGCGTGAAG TTTGCTGAGA TCTCGAACAA CTGGAACGGT 
GGAATTCGCT TTGGATTCAC CAGCAACGAT CCTGTAACGC TGGAGGGAAC GCTGCCCAAG 
TACGCCTGTC CGGATCTGAC CAACAGGCCT GGCTTCTGGG CCAAGGCTCT GCACGAGCAG 
TACTGCGAGA AGGACAACAT ACTTTACTAC TATGTCAATG GAGCTGGCGA TGTGATCTAT 
GGTATAAACA ACGAGGAGAA GGGTGTGATA CTGACCGGCA TCGATACGAG AAGCCTGCTG 
TGGACGGTGA TCGATATCTA TGGCAACTGC ACGGGCATTG AGTTTCTCGA TTCTCGCATT 
TACATGTACC AGCAGCAGCC AGCGGCTATA CCAATGGCCA CTGTGCCCGC CCAGCAACAG 
CAAATGCCCC AGCCAGCTGC CAATGCCTCC TCCGCCCTGA ATTCCCATCA CCCGCATCAG 
CAGTCGAGGA GATCCCTGCC AGGACACACT GCGGCCATCG AGCACGATCT GGAGCGCCAT 
GTGATGCCGT CCCTGCAATC CCTGCACTTG GCAGGAAACG GTGGAAGCGT GGCTAGCGTA 
GAACAGGCGG CCATTGCTCA TGATTTGGCC AATGGACTGC CTCCTCTGCG CTACAATGCC 
AACGGACGAC TGATTCCAGT GCCCTTCCAC AACACCAAGG GCCGGAATGT GCGGCTCTCG 
CAGGATCGCT TTGTGGCTTC TCGTACGGAA TCTGACTTCT GCCAGGGATA TGTGTTCACT 
GCCCGTCCCA TTCGCATTGG TGAGAAGCTG ATTGTGCAGG TCCTGAAAAC GGAACAGATG 
TACGTGGGTG CCTTGGCTTT GGGTCTGACC TCCTGCAATC CGGCCATGTT GCAGCCCAAT 
GATTTGCCCA ATGATTCGGA TTTCCTGCTC GATCGCCCCG AGTACTGGGT GGTGAGCAAG 
GATATTGCAG CTGCTCCTCA GCGCGGCGAT GAGATTGCCT TCTTCGTGGC GCCCAACGGT 
GAAGTGAGCA TCAGCAAGAA CAACGGACCA GCTGTGGTGG TGATGCATGT GGACCAGTCC 
CTCCAGCTGT GGGCCTTCCT GGATGTCTAC GGCTCGACGC AGTCTCTGAG GATGTTCCGC 
CAGCAGTTAC CCAACATGGT TGCGTATCCA TCGCAGCCGC AGGTCAATGT GAATGCCAGC 
AGCTCGTCCG CCTGCAACGC TGCCTCCACG TCCCGCATGC TGCCCATGAC CGAGTCGATG 
AGCAGTCTGA ATGCCGGAGC CACTGCCAAG CTGTTGCATC ATCCCTCGCA GCTGAGCGTA 
GCCCAGAGTA CGAGCACTTT GGCCTCAGCT GGCGGAGTCA ATGGCAGCCG CATGATCAGC 
ATGCCCTCCA ACGGAGATAT TCTCCAGATC CAGCCGAATG GTGGTGGCAC CGTTCTGGTG 
GTCAACCTGC CACCTGCTAG CAGCTCGCAC GATATAAACG GCCAACTCGC GGCCAGGCCA 
ACGGCCACGG TAACCTCCAG CGGTGTTCTG GCCGGAGCCT GCTCCAGCGG CACCCTTATC 
AGCACCACCA GCAGCCAGTA CATCGAGCAG CCCATTGCCA ATAGCACAAA TAATGCGGCC 
AACAAGTGGA AGGACAGCCT GAGCGACCAG CAGAGCACCG ATTCGAGTGC CGAATGCACC 
ATCTGCTACG AGAATCCCAT CGACTCGGTG CTGTACATGT GCGGACACAT GTGCATGTGC 
TACGACTGCG CCATCGAGCA GTGGCGCGGA GTGGGTGGTG GCCAGTGCCC ACTGTGCCGG 
GCGGTGATCC GGGACGTCAT CCGCACCTAC ACCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa04311
Clone ID Related Accession (Same CDS sequence) NM_169256.3 , NM_001170092.3
Accession Version NM_001170092.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product neuralized, isoform E
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170092.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGTCAGT CGGCTGGTAA AATAGTTCGC CGCTCACCCT CTTCATGTCC TGGCCCCAAC 
AACCTGCCTC CGCTGCAATT CCACTCCGTA CATGGCGACA ACATCCGAAT TTCAAGGGAC 
GGAACCCTGG CCCGTCGCTT TGAGAGCTTC TGCAGGGCCA TCACCTTTTC AGCGCGTCCG 
GTTCGCATCA ACGAGAGGAT CTGCGTGAAG TTTGCTGAGA TCTCGAACAA CTGGAACGGT 
GGAATTCGCT TTGGATTCAC CAGCAACGAT CCTGTAACGC TGGAGGGAAC GCTGCCCAAG 
TACGCCTGTC CGGATCTGAC CAACAGGCCT GGCTTCTGGG CCAAGGCTCT GCACGAGCAG 
TACTGCGAGA AGGACAACAT ACTTTACTAC TATGTCAATG GAGCTGGCGA TGTGATCTAT 
GGTATAAACA ACGAGGAGAA GGGTGTGATA CTGACCGGCA TCGATACGAG AAGCCTGCTG 
TGGACGGTGA TCGATATCTA TGGCAACTGC ACGGGCATTG AGTTTCTCGA TTCTCGCATT 
TACATGTACC AGCAGCAGCC AGCGGCTATA CCAATGGCCA CTGTGCCCGC CCAGCAACAG 
CAAATGCCCC AGCCAGCTGC CAATGCCTCC TCCGCCCTGA ATTCCCATCA CCCGCATCAG 
CAGTCGAGGA GATCCCTGCC AGGACACACT GCGGCCATCG AGCACGATCT GGAGCGCCAT 
GTGATGCCGT CCCTGCAATC CCTGCACTTG GCAGGAAACG GTGGAAGCGT GGCTAGCGTA 
GAACAGGCGG CCATTGCTCA TGATTTGGCC AATGGACTGC CTCCTCTGCG CTACAATGCC 
AACGGACGAC TGATTCCAGT GCCCTTCCAC AACACCAAGG GCCGGAATGT GCGGCTCTCG 
CAGGATCGCT TTGTGGCTTC TCGTACGGAA TCTGACTTCT GCCAGGGATA TGTGTTCACT 
GCCCGTCCCA TTCGCATTGG TGAGAAGCTG ATTGTGCAGG TCCTGAAAAC GGAACAGATG 
TACGTGGGTG CCTTGGCTTT GGGTCTGACC TCCTGCAATC CGGCCATGTT GCAGCCCAAT 
GATTTGCCCA ATGATTCGGA TTTCCTGCTC GATCGCCCCG AGTACTGGGT GGTGAGCAAG 
GATATTGCAG CTGCTCCTCA GCGCGGCGAT GAGATTGCCT TCTTCGTGGC GCCCAACGGT 
GAAGTGAGCA TCAGCAAGAA CAACGGACCA GCTGTGGTGG TGATGCATGT GGACCAGTCC 
CTCCAGCTGT GGGCCTTCCT GGATGTCTAC GGCTCGACGC AGTCTCTGAG GATGTTCCGC 
CAGCAGTTAC CCAACATGGT TGCGTATCCA TCGCAGCCGC AGGTCAATGT GAATGCCAGC 
AGCTCGTCCG CCTGCAACGC TGCCTCCACG TCCCGCATGC TGCCCATGAC CGAGTCGATG 
AGCAGTCTGA ATGCCGGAGC CACTGCCAAG CTGTTGCATC ATCCCTCGCA GCTGAGCGTA 
GCCCAGAGTA CGAGCACTTT GGCCTCAGCT GGCGGAGTCA ATGGCAGCCG CATGATCAGC 
ATGCCCTCCA ACGGAGATAT TCTCCAGATC CAGCCGAATG GTGGTGGCAC CGTTCTGGTG 
GTCAACCTGC CACCTGCTAG CAGCTCGCAC GATATAAACG GCCAACTCGC GGCCAGGCCA 
ACGGCCACGG TAACCTCCAG CGGTGTTCTG GCCGGAGCCT GCTCCAGCGG CACCCTTATC 
AGCACCACCA GCAGCCAGTA CATCGAGCAG CCCATTGCCA ATAGCACAAA TAATGCGGCC 
AACAAGTGGA AGGACAGCCT GAGCGACCAG CAGAGCACCG ATTCGAGTGC CGAATGCACC 
ATCTGCTACG AGAATCCCAT CGACTCGGTG CTGTACATGT GCGGACACAT GTGCATGTGC 
TACGACTGCG CCATCGAGCA GTGGCGCGGA GTGGGTGGTG GCCAGTGCCC ACTGTGCCGG 
GCGGTGATCC GGGACGTCAT CCGCACCTAC ACCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001163563.1
CDS293..2311
Translation

Target ORF information:

RefSeq Version NM_001170092.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster neuralized (neur), transcript variant E, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001170092.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGTCAGT CGGCTGGTAA AATAGTTCGC CGCTCACCCT CTTCATGTCC TGGCCCCAAC 
AACCTGCCTC CGCTGCAATT CCACTCCGTA CATGGCGACA ACATCCGAAT TTCAAGGGAC 
GGAACCCTGG CCCGTCGCTT TGAGAGCTTC TGCAGGGCCA TCACCTTTTC AGCGCGTCCG 
GTTCGCATCA ACGAGAGGAT CTGCGTGAAG TTTGCTGAGA TCTCGAACAA CTGGAACGGT 
GGAATTCGCT TTGGATTCAC CAGCAACGAT CCTGTAACGC TGGAGGGAAC GCTGCCCAAG 
TACGCCTGTC CGGATCTGAC CAACAGGCCT GGCTTCTGGG CCAAGGCTCT GCACGAGCAG 
TACTGCGAGA AGGACAACAT ACTTTACTAC TATGTCAATG GAGCTGGCGA TGTGATCTAT 
GGTATAAACA ACGAGGAGAA GGGTGTGATA CTGACCGGCA TCGATACGAG AAGCCTGCTG 
TGGACGGTGA TCGATATCTA TGGCAACTGC ACGGGCATTG AGTTTCTCGA TTCTCGCATT 
TACATGTACC AGCAGCAGCC AGCGGCTATA CCAATGGCCA CTGTGCCCGC CCAGCAACAG 
CAAATGCCCC AGCCAGCTGC CAATGCCTCC TCCGCCCTGA ATTCCCATCA CCCGCATCAG 
CAGTCGAGGA GATCCCTGCC AGGACACACT GCGGCCATCG AGCACGATCT GGAGCGCCAT 
GTGATGCCGT CCCTGCAATC CCTGCACTTG GCAGGAAACG GTGGAAGCGT GGCTAGCGTA 
GAACAGGCGG CCATTGCTCA TGATTTGGCC AATGGACTGC CTCCTCTGCG CTACAATGCC 
AACGGACGAC TGATTCCAGT GCCCTTCCAC AACACCAAGG GCCGGAATGT GCGGCTCTCG 
CAGGATCGCT TTGTGGCTTC TCGTACGGAA TCTGACTTCT GCCAGGGATA TGTGTTCACT 
GCCCGTCCCA TTCGCATTGG TGAGAAGCTG ATTGTGCAGG TCCTGAAAAC GGAACAGATG 
TACGTGGGTG CCTTGGCTTT GGGTCTGACC TCCTGCAATC CGGCCATGTT GCAGCCCAAT 
GATTTGCCCA ATGATTCGGA TTTCCTGCTC GATCGCCCCG AGTACTGGGT GGTGAGCAAG 
GATATTGCAG CTGCTCCTCA GCGCGGCGAT GAGATTGCCT TCTTCGTGGC GCCCAACGGT 
GAAGTGAGCA TCAGCAAGAA CAACGGACCA GCTGTGGTGG TGATGCATGT GGACCAGTCC 
CTCCAGCTGT GGGCCTTCCT GGATGTCTAC GGCTCGACGC AGTCTCTGAG GATGTTCCGC 
CAGCAGTTAC CCAACATGGT TGCGTATCCA TCGCAGCCGC AGGTCAATGT GAATGCCAGC 
AGCTCGTCCG CCTGCAACGC TGCCTCCACG TCCCGCATGC TGCCCATGAC CGAGTCGATG 
AGCAGTCTGA ATGCCGGAGC CACTGCCAAG CTGTTGCATC ATCCCTCGCA GCTGAGCGTA 
GCCCAGAGTA CGAGCACTTT GGCCTCAGCT GGCGGAGTCA ATGGCAGCCG CATGATCAGC 
ATGCCCTCCA ACGGAGATAT TCTCCAGATC CAGCCGAATG GTGGTGGCAC CGTTCTGGTG 
GTCAACCTGC CACCTGCTAG CAGCTCGCAC GATATAAACG GCCAACTCGC GGCCAGGCCA 
ACGGCCACGG TAACCTCCAG CGGTGTTCTG GCCGGAGCCT GCTCCAGCGG CACCCTTATC 
AGCACCACCA GCAGCCAGTA CATCGAGCAG CCCATTGCCA ATAGCACAAA TAATGCGGCC 
AACAAGTGGA AGGACAGCCT GAGCGACCAG CAGAGCACCG ATTCGAGTGC CGAATGCACC 
ATCTGCTACG AGAATCCCAT CGACTCGGTG CTGTACATGT GCGGACACAT GTGCATGTGC 
TACGACTGCG CCATCGAGCA GTGGCGCGGA GTGGGTGGTG GCCAGTGCCC ACTGTGCCGG 
GCGGTGATCC GGGACGTCAT CCGCACCTAC ACCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa04312
Clone ID Related Accession (Same CDS sequence) NM_169257.3
Accession Version NM_169257.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product neuralized, isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169257.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGGTCTAT CGGATATACC AGCCAACTAT ATGCAGGGCA GCCATCCGCA CCTAACTCTC 
CATCCGCAGC AGCAGCACCA CCAGAATCAG CAGCACTTGC AGCATCTGCA ACAGATGCAG 
CAGCTGCACA ACGCCATGCC CACTCCCGCC CAGCAGGCCG CCCAGGTCCT GGCCATGGAG 
TCCAACGAGC TGCTCATGAG CACCAAGGAC AAGCTCTCTA GCAAGAAGAA AATGCACTTG 
CTCAAGAAGA TCAAGAAGCG CTTTGGATTG GTTCGCCGCT CACCCTCTTC ATGTCCTGGC 
CCCAACAACC TGCCTCCGCT GCAATTCCAC TCCGTACATG GCGACAACAT CCGAATTTCA 
AGGGACGGAA CCCTGGCCCG TCGCTTTGAG AGCTTCTGCA GGGCCATCAC CTTTTCAGCG 
CGTCCGGTTC GCATCAACGA GAGGATCTGC GTGAAGTTTG CTGAGATCTC GAACAACTGG 
AACGGTGGAA TTCGCTTTGG ATTCACCAGC AACGATCCTG TAACGCTGGA GGGAACGCTG 
CCCAAGTACG CCTGTCCGGA TCTGACCAAC AGGCCTGGCT TCTGGGCCAA GGCTCTGCAC 
GAGCAGTACT GCGAGAAGGA CAACATACTT TACTACTATG TCAATGGAGC TGGCGATGTG 
ATCTATGGTA TAAACAACGA GGAGAAGGGT GTGATACTGA CCGGCATCGA TACGAGAAGC 
CTGCTGTGGA CGGTGATCGA TATCTATGGC AACTGCACGG GCATTGAGTT TCTCGATTCT 
CGCATTTACA TGTACCAGCA GCAGCCAGCG GCTATACCAA TGGCCACTGT GCCCGCCCAG 
CAACAGCAAA TGCCCCAGCC AGCTGCCAAT GCCTCCTCCG CCCTGAATTC CCATCACCCG 
CATCAGCAGT CGAGGAGATC CCTGCCAGGA CACACTGCGG CCATCGAGCA CGATCTGGAG 
CGCCATGTGA TGCCGTCCCT GCAATCCCTG CACTTGGCAG GAAACGGTGG AAGCGTGGCT 
AGCGTAGAAC AGGCGGCCAT TGCTCATGAT TTGGCCAATG GACTGCCTCC TCTGCGCTAC 
AATGCCAACG GACGACTGAT TCCAGTGCCC TTCCACAACA CCAAGGGCCG GAATGTGCGG 
CTCTCGCAGG ATCGCTTTGT GGCTTCTCGT ACGGAATCTG ACTTCTGCCA GGGATATGTG 
TTCACTGCCC GTCCCATTCG CATTGGTGAG AAGCTGATTG TGCAGGTCCT GAAAACGGAA 
CAGATGTACG TGGGTGCCTT GGCTTTGGGT CTGACCTCCT GCAATCCGGC CATGTTGCAG 
CCCAATGATT TGCCCAATGA TTCGGATTTC CTGCTCGATC GCCCCGAGTA CTGGGTGGTG 
AGCAAGGATA TTGCAGCTGC TCCTCAGCGC GGCGATGAGA TTGCCTTCTT CGTGGCGCCC 
AACGGTGAAG TGAGCATCAG CAAGAACAAC GGACCAGCTG TGGTGGTGAT GCATGTGGAC 
CAGTCCCTCC AGCTGTGGGC CTTCCTGGAT GTCTACGGCT CGACGCAGTC TCTGAGGATG 
TTCCGCCAGC AGTTACCCAA CATGGTTGCG TATCCATCGC AGCCGCAGGT CAATGTGAAT 
GCCAGCAGCT CGTCCGCCTG CAACGCTGCC TCCACGTCCC GCATGCTGCC CATGACCGAG 
TCGATGAGCA GTCTGAATGC CGGAGCCACT GCCAAGCTGT TGCATCATCC CTCGCAGCTG 
AGCGTAGCCC AGAGTACGAG CACTTTGGCC TCAGCTGGCG GAGTCAATGG CAGCCGCATG 
ATCAGCATGC CCTCCAACGG AGATATTCTC CAGATCCAGC CGAATGGTGG TGGCACCGTT 
CTGGTGGTCA ACCTGCCACC TGCTAGCAGC TCGCACGATA TAAACGGCCA ACTCGCGGCC 
AGGCCAACGG CCACGGTAAC CTCCAGCGGT GTTCTGGCCG GAGCCTGCTC CAGCGGCACC 
CTTATCAGCA CCACCAGCAG CCAGTACATC GAGCCCATTG CCAATAGCAC AAATAATGCG 
GCCAACAAGT GGAAGGACAG CCTGAGCGAC CAGCAGAGCA CCGATTCGAG TGCCGAATGC 
ACCATCTGCT ACGAGAATCC CATCGACTCG GTGCTGTACA TGTGCGGACA CATGTGCATG 
TGCTACGACT GCGCCATCGA GCAGTGGCGC GGAGTGGGTG GTGGCCAGTG CCCACTGTGC 
CGGGCGGTGA TCCGGGACGT CATCCGCACC TACACCACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_731311.1
CDS283..2544
Translation

Target ORF information:

RefSeq Version NM_169257.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster neuralized (neur), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_169257.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGGTCTAT CGGATATACC AGCCAACTAT ATGCAGGGCA GCCATCCGCA CCTAACTCTC 
CATCCGCAGC AGCAGCACCA CCAGAATCAG CAGCACTTGC AGCATCTGCA ACAGATGCAG 
CAGCTGCACA ACGCCATGCC CACTCCCGCC CAGCAGGCCG CCCAGGTCCT GGCCATGGAG 
TCCAACGAGC TGCTCATGAG CACCAAGGAC AAGCTCTCTA GCAAGAAGAA AATGCACTTG 
CTCAAGAAGA TCAAGAAGCG CTTTGGATTG GTTCGCCGCT CACCCTCTTC ATGTCCTGGC 
CCCAACAACC TGCCTCCGCT GCAATTCCAC TCCGTACATG GCGACAACAT CCGAATTTCA 
AGGGACGGAA CCCTGGCCCG TCGCTTTGAG AGCTTCTGCA GGGCCATCAC CTTTTCAGCG 
CGTCCGGTTC GCATCAACGA GAGGATCTGC GTGAAGTTTG CTGAGATCTC GAACAACTGG 
AACGGTGGAA TTCGCTTTGG ATTCACCAGC AACGATCCTG TAACGCTGGA GGGAACGCTG 
CCCAAGTACG CCTGTCCGGA TCTGACCAAC AGGCCTGGCT TCTGGGCCAA GGCTCTGCAC 
GAGCAGTACT GCGAGAAGGA CAACATACTT TACTACTATG TCAATGGAGC TGGCGATGTG 
ATCTATGGTA TAAACAACGA GGAGAAGGGT GTGATACTGA CCGGCATCGA TACGAGAAGC 
CTGCTGTGGA CGGTGATCGA TATCTATGGC AACTGCACGG GCATTGAGTT TCTCGATTCT 
CGCATTTACA TGTACCAGCA GCAGCCAGCG GCTATACCAA TGGCCACTGT GCCCGCCCAG 
CAACAGCAAA TGCCCCAGCC AGCTGCCAAT GCCTCCTCCG CCCTGAATTC CCATCACCCG 
CATCAGCAGT CGAGGAGATC CCTGCCAGGA CACACTGCGG CCATCGAGCA CGATCTGGAG 
CGCCATGTGA TGCCGTCCCT GCAATCCCTG CACTTGGCAG GAAACGGTGG AAGCGTGGCT 
AGCGTAGAAC AGGCGGCCAT TGCTCATGAT TTGGCCAATG GACTGCCTCC TCTGCGCTAC 
AATGCCAACG GACGACTGAT TCCAGTGCCC TTCCACAACA CCAAGGGCCG GAATGTGCGG 
CTCTCGCAGG ATCGCTTTGT GGCTTCTCGT ACGGAATCTG ACTTCTGCCA GGGATATGTG 
TTCACTGCCC GTCCCATTCG CATTGGTGAG AAGCTGATTG TGCAGGTCCT GAAAACGGAA 
CAGATGTACG TGGGTGCCTT GGCTTTGGGT CTGACCTCCT GCAATCCGGC CATGTTGCAG 
CCCAATGATT TGCCCAATGA TTCGGATTTC CTGCTCGATC GCCCCGAGTA CTGGGTGGTG 
AGCAAGGATA TTGCAGCTGC TCCTCAGCGC GGCGATGAGA TTGCCTTCTT CGTGGCGCCC 
AACGGTGAAG TGAGCATCAG CAAGAACAAC GGACCAGCTG TGGTGGTGAT GCATGTGGAC 
CAGTCCCTCC AGCTGTGGGC CTTCCTGGAT GTCTACGGCT CGACGCAGTC TCTGAGGATG 
TTCCGCCAGC AGTTACCCAA CATGGTTGCG TATCCATCGC AGCCGCAGGT CAATGTGAAT 
GCCAGCAGCT CGTCCGCCTG CAACGCTGCC TCCACGTCCC GCATGCTGCC CATGACCGAG 
TCGATGAGCA GTCTGAATGC CGGAGCCACT GCCAAGCTGT TGCATCATCC CTCGCAGCTG 
AGCGTAGCCC AGAGTACGAG CACTTTGGCC TCAGCTGGCG GAGTCAATGG CAGCCGCATG 
ATCAGCATGC CCTCCAACGG AGATATTCTC CAGATCCAGC CGAATGGTGG TGGCACCGTT 
CTGGTGGTCA ACCTGCCACC TGCTAGCAGC TCGCACGATA TAAACGGCCA ACTCGCGGCC 
AGGCCAACGG CCACGGTAAC CTCCAGCGGT GTTCTGGCCG GAGCCTGCTC CAGCGGCACC 
CTTATCAGCA CCACCAGCAG CCAGTACATC GAGCCCATTG CCAATAGCAC AAATAATGCG 
GCCAACAAGT GGAAGGACAG CCTGAGCGAC CAGCAGAGCA CCGATTCGAG TGCCGAATGC 
ACCATCTGCT ACGAGAATCC CATCGACTCG GTGCTGTACA TGTGCGGACA CATGTGCATG 
TGCTACGACT GCGCCATCGA GCAGTGGCGC GGAGTGGGTG GTGGCCAGTG CCCACTGTGC 
CGGGCGGTGA TCCGGGACGT CATCCGCACC TACACCACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa04313
Clone ID Related Accession (Same CDS sequence) NM_057304.5
Accession Version NM_057304.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product neuralized, isoform A
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057304.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGGTCTAT CGGATATACC AGCCAACTAT ATGCAGGGCA GCCATCCGCA CCTAACTCTC 
CATCCGCAGC AGCAGCACCA CCAGAATCAG CAGCACTTGC AGCATCTGCA ACAGATGCAG 
CAGCTGCACA ACGCCATGCC CACTCCCGCC CAGCAGGCCG CCCAGGTCCT GGCCATGGAG 
TCCAACGAGC TGCTCATGAG CACCAAGGAC AAGCTCTCTA GCAAGAAGAA AATGCACTTG 
CTCAAGAAGA TCAAGAAGCG CTTTGGATTG GTTCGCCGCT CACCCTCTTC ATGTCCTGGC 
CCCAACAACC TGCCTCCGCT GCAATTCCAC TCCGTACATG GCGACAACAT CCGAATTTCA 
AGGGACGGAA CCCTGGCCCG TCGCTTTGAG AGCTTCTGCA GGGCCATCAC CTTTTCAGCG 
CGTCCGGTTC GCATCAACGA GAGGATCTGC GTGAAGTTTG CTGAGATCTC GAACAACTGG 
AACGGTGGAA TTCGCTTTGG ATTCACCAGC AACGATCCTG TAACGCTGGA GGGAACGCTG 
CCCAAGTACG CCTGTCCGGA TCTGACCAAC AGGCCTGGCT TCTGGGCCAA GGCTCTGCAC 
GAGCAGTACT GCGAGAAGGA CAACATACTT TACTACTATG TCAATGGAGC TGGCGATGTG 
ATCTATGGTA TAAACAACGA GGAGAAGGGT GTGATACTGA CCGGCATCGA TACGAGAAGC 
CTGCTGTGGA CGGTGATCGA TATCTATGGC AACTGCACGG GCATTGAGTT TCTCGATTCT 
CGCATTTACA TGTACCAGCA GCAGCCAGCG GCTATACCAA TGGCCACTGT GCCCGCCCAG 
CAACAGCAAA TGCCCCAGCC AGCTGCCAAT GCCTCCTCCG CCCTGAATTC CCATCACCCG 
CATCAGCAGT CGAGGAGATC CCTGCCAGGA CACACTGCGG CCATCGAGCA CGATCTGGAG 
CGCCATGTGA TGCCGTCCCT GCAATCCCTG CACTTGGCAG GAAACGGTGG AAGCGTGGCT 
AGCGTAGAAC AGGCGGCCAT TGCTCATGAT TTGGCCAATG GACTGCCTCC TCTGCGCTAC 
AATGCCAACG GACGACTGAT TCCAGTGCCC TTCCACAACA CCAAGGGCCG GAATGTGCGG 
CTCTCGCAGG ATCGCTTTGT GGCTTCTCGT ACGGAATCTG ACTTCTGCCA GGGATATGTG 
TTCACTGCCC GTCCCATTCG CATTGGTGAG AAGCTGATTG TGCAGGTCCT GAAAACGGAA 
CAGATGTACG TGGGTGCCTT GGCTTTGGGT CTGACCTCCT GCAATCCGGC CATGTTGCAG 
CCCAATGATT TGCCCAATGA TTCGGATTTC CTGCTCGATC GCCCCGAGTA CTGGGTGGTG 
AGCAAGGATA TTGCAGCTGC TCCTCAGCGC GGCGATGAGA TTGCCTTCTT CGTGGCGCCC 
AACGGTGAAG TGAGCATCAG CAAGAACAAC GGACCAGCTG TGGTGGTGAT GCATGTGGAC 
CAGTCCCTCC AGCTGTGGGC CTTCCTGGAT GTCTACGGCT CGACGCAGTC TCTGAGGATG 
TTCCGCCAGC AGTTACCCAA CATGGTTGCG TATCCATCGC AGCCGCAGGT CAATGTGAAT 
GCCAGCAGCT CGTCCGCCTG CAACGCTGCC TCCACGTCCC GCATGCTGCC CATGACCGAG 
TCGATGAGCA GTCTGAATGC CGGAGCCACT GCCAAGCTGT TGCATCATCC CTCGCAGCTG 
AGCGTAGCCC AGAGTACGAG CACTTTGGCC TCAGCTGGCG GAGTCAATGG CAGCCGCATG 
ATCAGCATGC CCTCCAACGG AGATATTCTC CAGATCCAGC CGAATGGTGG TGGCACCGTT 
CTGGTGGTCA ACCTGCCACC TGCTAGCAGC TCGCACGATA TAAACGGCCA ACTCGCGGCC 
AGGCCAACGG CCACGGTAAC CTCCAGCGGT GTTCTGGCCG GAGCCTGCTC CAGCGGCACC 
CTTATCAGCA CCACCAGCAG CCAGTACATC GAGCAGCCCA TTGCCAATAG CACAAATAAT 
GCGGCCAACA AGTGGAAGGA CAGCCTGAGC GACCAGCAGA GCACCGATTC GAGTGCCGAA 
TGCACCATCT GCTACGAGAA TCCCATCGAC TCGGTGCTGT ACATGTGCGG ACACATGTGC 
ATGTGCTACG ACTGCGCCAT CGAGCAGTGG CGCGGAGTGG GTGGTGGCCA GTGCCCACTG 
TGCCGGGCGG TGATCCGGGA CGTCATCCGC ACCTACACCA CGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_476652.1
CDS283..2547
Translation

Target ORF information:

RefSeq Version NM_057304.5
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster neuralized (neur), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_057304.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGGTCTAT CGGATATACC AGCCAACTAT ATGCAGGGCA GCCATCCGCA CCTAACTCTC 
CATCCGCAGC AGCAGCACCA CCAGAATCAG CAGCACTTGC AGCATCTGCA ACAGATGCAG 
CAGCTGCACA ACGCCATGCC CACTCCCGCC CAGCAGGCCG CCCAGGTCCT GGCCATGGAG 
TCCAACGAGC TGCTCATGAG CACCAAGGAC AAGCTCTCTA GCAAGAAGAA AATGCACTTG 
CTCAAGAAGA TCAAGAAGCG CTTTGGATTG GTTCGCCGCT CACCCTCTTC ATGTCCTGGC 
CCCAACAACC TGCCTCCGCT GCAATTCCAC TCCGTACATG GCGACAACAT CCGAATTTCA 
AGGGACGGAA CCCTGGCCCG TCGCTTTGAG AGCTTCTGCA GGGCCATCAC CTTTTCAGCG 
CGTCCGGTTC GCATCAACGA GAGGATCTGC GTGAAGTTTG CTGAGATCTC GAACAACTGG 
AACGGTGGAA TTCGCTTTGG ATTCACCAGC AACGATCCTG TAACGCTGGA GGGAACGCTG 
CCCAAGTACG CCTGTCCGGA TCTGACCAAC AGGCCTGGCT TCTGGGCCAA GGCTCTGCAC 
GAGCAGTACT GCGAGAAGGA CAACATACTT TACTACTATG TCAATGGAGC TGGCGATGTG 
ATCTATGGTA TAAACAACGA GGAGAAGGGT GTGATACTGA CCGGCATCGA TACGAGAAGC 
CTGCTGTGGA CGGTGATCGA TATCTATGGC AACTGCACGG GCATTGAGTT TCTCGATTCT 
CGCATTTACA TGTACCAGCA GCAGCCAGCG GCTATACCAA TGGCCACTGT GCCCGCCCAG 
CAACAGCAAA TGCCCCAGCC AGCTGCCAAT GCCTCCTCCG CCCTGAATTC CCATCACCCG 
CATCAGCAGT CGAGGAGATC CCTGCCAGGA CACACTGCGG CCATCGAGCA CGATCTGGAG 
CGCCATGTGA TGCCGTCCCT GCAATCCCTG CACTTGGCAG GAAACGGTGG AAGCGTGGCT 
AGCGTAGAAC AGGCGGCCAT TGCTCATGAT TTGGCCAATG GACTGCCTCC TCTGCGCTAC 
AATGCCAACG GACGACTGAT TCCAGTGCCC TTCCACAACA CCAAGGGCCG GAATGTGCGG 
CTCTCGCAGG ATCGCTTTGT GGCTTCTCGT ACGGAATCTG ACTTCTGCCA GGGATATGTG 
TTCACTGCCC GTCCCATTCG CATTGGTGAG AAGCTGATTG TGCAGGTCCT GAAAACGGAA 
CAGATGTACG TGGGTGCCTT GGCTTTGGGT CTGACCTCCT GCAATCCGGC CATGTTGCAG 
CCCAATGATT TGCCCAATGA TTCGGATTTC CTGCTCGATC GCCCCGAGTA CTGGGTGGTG 
AGCAAGGATA TTGCAGCTGC TCCTCAGCGC GGCGATGAGA TTGCCTTCTT CGTGGCGCCC 
AACGGTGAAG TGAGCATCAG CAAGAACAAC GGACCAGCTG TGGTGGTGAT GCATGTGGAC 
CAGTCCCTCC AGCTGTGGGC CTTCCTGGAT GTCTACGGCT CGACGCAGTC TCTGAGGATG 
TTCCGCCAGC AGTTACCCAA CATGGTTGCG TATCCATCGC AGCCGCAGGT CAATGTGAAT 
GCCAGCAGCT CGTCCGCCTG CAACGCTGCC TCCACGTCCC GCATGCTGCC CATGACCGAG 
TCGATGAGCA GTCTGAATGC CGGAGCCACT GCCAAGCTGT TGCATCATCC CTCGCAGCTG 
AGCGTAGCCC AGAGTACGAG CACTTTGGCC TCAGCTGGCG GAGTCAATGG CAGCCGCATG 
ATCAGCATGC CCTCCAACGG AGATATTCTC CAGATCCAGC CGAATGGTGG TGGCACCGTT 
CTGGTGGTCA ACCTGCCACC TGCTAGCAGC TCGCACGATA TAAACGGCCA ACTCGCGGCC 
AGGCCAACGG CCACGGTAAC CTCCAGCGGT GTTCTGGCCG GAGCCTGCTC CAGCGGCACC 
CTTATCAGCA CCACCAGCAG CCAGTACATC GAGCAGCCCA TTGCCAATAG CACAAATAAT 
GCGGCCAACA AGTGGAAGGA CAGCCTGAGC GACCAGCAGA GCACCGATTC GAGTGCCGAA 
TGCACCATCT GCTACGAGAA TCCCATCGAC TCGGTGCTGT ACATGTGCGG ACACATGTGC 
ATGTGCTACG ACTGCGCCAT CGAGCAGTGG CGCGGAGTGG GTGGTGGCCA GTGCCCACTG 
TGCCGGGCGG TGATCCGGGA CGTCATCCGC ACCTACACCA CGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,


book

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,


book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH


book

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D