VAChT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	VAChT
Entrez Gene ID	42795
Full Name	Vesicular acetylcholine transporter
Synonyms	CG12345,CG32848,CT41182,Chn,Dmel\CG32848,VACHT,VACht,VaChT,Vacht,chn,vAChT,vacht
General protein information	Preferred Names Vesicular acetylcholine transporter Names CG32848 gene product from transcript CG32848-RA CG32848-PA Cha's neighbour VAChT-PA vesicular acetylcholine transporter
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91C7-97D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057790.5	NP_477138.1	vesicular acetylcholine transporter

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLC18A3	XP_001138996.1
Rattus norvegicus (Norway rat)	Slc18a3	NP_113851.1
Danio rerio (zebrafish)	slc18a3b	NP_957401.1
Caenorhabditis elegans (roundworm)	unc-17	NP_001023602.1
Xenopus tropicalis (tropical clawed frog)	slc18a3	NP_001072859.1
	SLC18A3	XP_001107292.1
Bos taurus (cattle)	SLC18A3	XP_002699016.1
Gallus gallus (chicken)	SLC18A3	NP_996865.1
Danio rerio (zebrafish)	slc18a3a	NP_001071018.1
Homo sapiens (human)	SLC18A3	NP_003046.2
Canis lupus familiaris (dog)	SLC18A3	XP_543901.2
Mus musculus (house mouse)	Slc18a3	NP_068358.2
Drosophila melanogaster (fruit fly)	VAChT	NP_477138.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002369	XP_312587.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VAChT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAChT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05816	NM_057790.5 Latest version!	Drosophila melanogaster vesicular acetylcholine transporter (VAChT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VAChT ( NM_057790.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477138.1 Drosophila melanogaster vesicular acetylcholine transporter (VAChT), mRNA.

Price & Availability↑

CloneID	OFa05816
Clone ID Related Accession (Same CDS sequence)	NM_057790.5
Accession Version	NM_057790.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vesicular acetylcholine transporter
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057790.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCAT TCCAAATACC TGTTATCAAC CTGGAGGTGC GCGAGGTCAA GGACATCGTG 
TGGGAGAAGA TCCAGGAGCC GGTGAACCAG CGACGCCTCA TCCTCGTGAT TGTATCGATA 
GCCCTGCTGC TGGACAACAT GCTGTATATG GTGATAGTAC CGATTATACC CGACTATTTA 
AGAGAGATTG GCAGCTTCGA TGACGGGCCG ACGCCTCCAC CGTTGAGGGA TAATATCACG 
GGAAAGATCA TACCCGTGCA TCATGATCAT CACGGGCAGG ACTCGGCCAC GGGCATCCTA 
TTCGCCTCGA AGGCCATCGT CCAGCTGATG GTGAATCCGT TTTCGGGCGG CCTCATCGAC 
AAGATCGGCT ACGATTTGCC CATGATGATC GGCCTGACCA TCATGTTCTT CTCGACGGCA 
GTCTTTGCTT GTGGCAGCAG CTACAGTGTC CTGTTCTTCG CCCGATCCCT GCAGGGAGCC 
GGTTCCGCCT TTGCGGACAC CGCCGGTTTG GCCATGATAG CCGATCGGTT CACCGAGGAG 
AACGAACGTT CGCAGGCCCT TGGCATTGCG TTGGCCTTCA TCAGTTTCGG ATGCCTGGTG 
GCGCCTCCGT TTGGCGGAGC GCTCTACCAG TTCGCCGGAA AGGAAGTGCC CTTTCTGATC 
CTGGCACTGG TGTGCCTACT GGACGGGCTG ATGCTGCTGC TGGTGATGAA ACCGGTCAAG 
GAGGCGATGA AGCAAAGCAA GGATGTGCAG GACCAAGTAA TACCCATCTG GCGCCTGCTG 
ATGGATCCCT ATATTGCAGT CTGTGCCGGG GCACTGACCA TGTCCAATGT GGCGCTGGCC 
TTTCTGGAGC CAACCATTTC GCTGTGGATG GAGGATAACA TGACCACTGA CAACTGGAAG 
ATCGGCATGG TCTGGCTGCC CGCCTTCTTC CCACACGTGC TGGGCGTGGT CATTACGGTG 
AAGATGGCCC GCAAGTATCC GCAGCACCAG TGGCTAATGG CTGCCGGTGG ATTGGCCCTG 
GAGGGCTTCT CCTGCTTCAT CATACCATTC TGCAGCGGCT ACAAGATGCT CATGCTGCCC 
ATCTGTGTGA TCTGCTTCGG AATTGCCCTA ATAGACACGG CTCTACTACC CACACTCGGT 
TATCTGGTGG ACGTGCGATA TGTGTCGGTG TACGGTAGTA TCTATGCCAT AGCAGATATA 
TCCTACTCGA TCGCCTATGC CGTAGGGCCC ATTATCGCCG GCGGAGTGGT GGAGGCCATT 
GGGTTTACGG CTCTCAACTT TTTGATAGCC TTCTCGAATC TGGCCTATGT GCCCGTGCTG 
CGAAAGCTGC GCAACATCTA CGACTTCAAG CCGTTCGAGA ATGAGGCCAA CATCCTGATG 
CAGGACCCGC CCAACAAAGA GTACCAGACC TATGTGATGC ACGACCAGAA ACCCGTCGAG 
GGTGGCGTCA AGAATCATCT GGAGTACGGC CAGCAGTATC AGCAGGAGCA GGAGACGAAT 
CTGGATGACC AGCAGTACGA GTACCAGCAG CAGCAGCAGG GATACCAGCA AGGATATCAG 
CAGGATCAGG GCTACCAGCC GGGCTATCAG GAGCAGGGCG GCAGCTACGC CCCGCAAGGT 
CAGCCCCGTG TGGCCAATCC CTTCCAGCAG CAGCAGCAGC AGCAACAACA ACAACAGCAG 
CAGGTCCAGA GCAGAGGTCC TGCCGCCCCA GCGAATCCCT TTAGGCAAGG ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477138.1
CDS	992..2728
Translation	MASFQIPVINLEVREVKDIVWEKIQEPVNQRRLILVIVSIALLLDNMLYMVIVPIIPDYLREIGSFDDGPTPPPLRDNITGKIIPVHHDHHGQDSATGILFASKAIVQLMVNPFSGGLIDKIGYDLPMMIGLTIMFFSTAVFACGSSYSVLFFARSLQGAGSAFADTAGLAMIADRFTEENERSQALGIALAFISFGCLVAPPFGGALYQFAGKEVPFLILALVCLLDGLMLLLVMKPVKEAMKQSKDVQDQVIPIWRLLMDPYIAVCAGALTMSNVALAFLEPTISLWMEDNMTTDNWKIGMVWLPAFFPHVLGVVITVKMARKYPQHQWLMAAGGLALEGFSCFIIPFCSGYKMLMLPICVICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSIAYAVGPIIAGGVVEAIGFTALNFLIAFSNLAYVPVLRKLRNIYDFKPFENEANILMQDPPNKEYQTYVMHDQKPVEGGVKNHLEYGQQYQQEQETNLDDQQYEYQQQQQGYQQGYQQDQGYQPGYQEQGGSYAPQGQPRVANPFQQQQQQQQQQQQQVQSRGPAAPANPFRQGF

Target ORF information:

RefSeq Version	NM_057790.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vesicular acetylcholine transporter (VAChT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057790.5

ORF Insert Sequence:

ATGGCCTCAT TCCAAATACC TGTTATCAAC CTGGAGGTGC GCGAGGTCAA GGACATCGTG 
TGGGAGAAGA TCCAGGAGCC GGTGAACCAG CGACGCCTCA TCCTCGTGAT TGTATCGATA 
GCCCTGCTGC TGGACAACAT GCTGTATATG GTGATAGTAC CGATTATACC CGACTATTTA 
AGAGAGATTG GCAGCTTCGA TGACGGGCCG ACGCCTCCAC CGTTGAGGGA TAATATCACG 
GGAAAGATCA TACCCGTGCA TCATGATCAT CACGGGCAGG ACTCGGCCAC GGGCATCCTA 
TTCGCCTCGA AGGCCATCGT CCAGCTGATG GTGAATCCGT TTTCGGGCGG CCTCATCGAC 
AAGATCGGCT ACGATTTGCC CATGATGATC GGCCTGACCA TCATGTTCTT CTCGACGGCA 
GTCTTTGCTT GTGGCAGCAG CTACAGTGTC CTGTTCTTCG CCCGATCCCT GCAGGGAGCC 
GGTTCCGCCT TTGCGGACAC CGCCGGTTTG GCCATGATAG CCGATCGGTT CACCGAGGAG 
AACGAACGTT CGCAGGCCCT TGGCATTGCG TTGGCCTTCA TCAGTTTCGG ATGCCTGGTG 
GCGCCTCCGT TTGGCGGAGC GCTCTACCAG TTCGCCGGAA AGGAAGTGCC CTTTCTGATC 
CTGGCACTGG TGTGCCTACT GGACGGGCTG ATGCTGCTGC TGGTGATGAA ACCGGTCAAG 
GAGGCGATGA AGCAAAGCAA GGATGTGCAG GACCAAGTAA TACCCATCTG GCGCCTGCTG 
ATGGATCCCT ATATTGCAGT CTGTGCCGGG GCACTGACCA TGTCCAATGT GGCGCTGGCC 
TTTCTGGAGC CAACCATTTC GCTGTGGATG GAGGATAACA TGACCACTGA CAACTGGAAG 
ATCGGCATGG TCTGGCTGCC CGCCTTCTTC CCACACGTGC TGGGCGTGGT CATTACGGTG 
AAGATGGCCC GCAAGTATCC GCAGCACCAG TGGCTAATGG CTGCCGGTGG ATTGGCCCTG 
GAGGGCTTCT CCTGCTTCAT CATACCATTC TGCAGCGGCT ACAAGATGCT CATGCTGCCC 
ATCTGTGTGA TCTGCTTCGG AATTGCCCTA ATAGACACGG CTCTACTACC CACACTCGGT 
TATCTGGTGG ACGTGCGATA TGTGTCGGTG TACGGTAGTA TCTATGCCAT AGCAGATATA 
TCCTACTCGA TCGCCTATGC CGTAGGGCCC ATTATCGCCG GCGGAGTGGT GGAGGCCATT 
GGGTTTACGG CTCTCAACTT TTTGATAGCC TTCTCGAATC TGGCCTATGT GCCCGTGCTG 
CGAAAGCTGC GCAACATCTA CGACTTCAAG CCGTTCGAGA ATGAGGCCAA CATCCTGATG 
CAGGACCCGC CCAACAAAGA GTACCAGACC TATGTGATGC ACGACCAGAA ACCCGTCGAG 
GGTGGCGTCA AGAATCATCT GGAGTACGGC CAGCAGTATC AGCAGGAGCA GGAGACGAAT 
CTGGATGACC AGCAGTACGA GTACCAGCAG CAGCAGCAGG GATACCAGCA AGGATATCAG 
CAGGATCAGG GCTACCAGCC GGGCTATCAG GAGCAGGGCG GCAGCTACGC CCCGCAAGGT 
CAGCCCCGTG TGGCCAATCC CTTCCAGCAG CAGCAGCAGC AGCAACAACA ACAACAGCAG 
CAGGTCCAGA GCAGAGGTCC TGCCGCCCCA GCGAATCCCT TTAGGCAAGG ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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VAChT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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