COL4A5 cDNA ORF clone, Homo sapiens(human)

The following COL4A5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16745 NM_033380.3
Latest version!
Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu16784 NM_000495.5
Latest version!
Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu99143 XM_011530849.2
Latest version!
Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu99144 XM_017029259.2
Latest version!
Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu99145 XM_017029260.1
Latest version!
Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu99146 XM_017029261.1
Latest version!
Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu99147 XM_017029262.2
Latest version!
Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu99148 XM_017029263.2
Latest version!
Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
View Old Accession Versions >>
OHu16745 NM_033380.2 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu16784 NM_000495.4 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu99144 XM_017029259.1 Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu99148 XM_017029263.1 Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu99147 XM_017029262.1 Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16745
    Clone ID Related Accession (Same CDS sequence) NM_033380.2 , NM_033380.3
    Accession Version NM_033380.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461056.1, BC151846.1, BC035387.1, M31115.1 and BU688037.1. On Dec 30, 2011 this sequence version replaced NM_033380.1. Transcript Variant: This variant (2) encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC
    AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT
    ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC
    CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA
    CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC
    ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA
    TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA
    CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT
    GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA
    TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT
    TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT
    GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG
    GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG
    GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC
    CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA
    GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG
    CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG
    CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA
    GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT
    CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA
    ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT
    GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA
    GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT
    CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG
    GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA
    AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA
    GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG
    GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT
    CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG
    GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG
    AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA
    GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT
    TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT
    CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC TCCAGGTCTC
    CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAATCCAG GCCAACCTGG GCTACCTGGC
    TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGCCGGCCG
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGATCAGCT GGCCCTGAGG GGGAACCGGG ACTTATTGGT
    CCTCCAGGTC CTCCTGGATT ACCTGGTCCT TCAGGACAGA GTATCATAAT TAAAGGAGAT
    GCTGGTCCTC CAGGAATCCC TGGCCAGCCT GGGCTAAAGG GTCTACCAGG ACCCCAAGGA
    CCTCAAGGCT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGTACC
    CGTGGTTTGG ATGGTCCCCC TGGTCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA
    ACCTCCTCTG TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA
    CAATGCCCAC AGGGAACACT TCAGGTCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CCCACGGTCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC
    TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC
    GCAGTTCACA GTCAGACGAT CCAGATTCCC CATTGTCCTC AGGGATGGGA TTCTCTGTGG
    ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA
    GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG
    AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG
    TCAGACATGT TCAGTAAACC TCAGTCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGA
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_203699.1
    CDS283..5358
    Translation

    Target ORF information:

    RefSeq Version NM_033380.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033380.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC
    AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT
    ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC
    CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA
    CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC
    ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA
    TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA
    CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT
    GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA
    TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT
    TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT
    GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG
    GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG
    GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC
    CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA
    GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG
    CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG
    CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA
    GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT
    CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA
    ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT
    GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA
    GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT
    CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG
    GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA
    AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA
    GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG
    GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT
    CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG
    GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG
    AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA
    GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT
    TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT
    CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC TCCAGGTCTC
    CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAATCCAG GCCAACCTGG GCTACCTGGC
    TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGCCGGCCG
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGATCAGCT GGCCCTGAGG GGGAACCGGG ACTTATTGGT
    CCTCCAGGTC CTCCTGGATT ACCTGGTCCT TCAGGACAGA GTATCATAAT TAAAGGAGAT
    GCTGGTCCTC CAGGAATCCC TGGCCAGCCT GGGCTAAAGG GTCTACCAGG ACCCCAAGGA
    CCTCAAGGCT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGTACC
    CGTGGTTTGG ATGGTCCCCC TGGTCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA
    ACCTCCTCTG TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA
    CAATGCCCAC AGGGAACACT TCAGGTCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CCCACGGTCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC
    TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC
    GCAGTTCACA GTCAGACGAT CCAGATTCCC CATTGTCCTC AGGGATGGGA TTCTCTGTGG
    ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA
    GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG
    AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG
    TCAGACATGT TCAGTAAACC TCAGTCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGA
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16745
    Clone ID Related Accession (Same CDS sequence) NM_033380.2 , NM_033380.3
    Accession Version NM_033380.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, DA461056.1, BC151846.1, BC035387.1, M31115.1 and BU688037.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_033380.2. Transcript Variant: This variant (2) encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC
    AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT
    ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC
    CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA
    CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC
    ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA
    TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA
    CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT
    GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA
    TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT
    TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT
    GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG
    GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG
    GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC
    CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA
    GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG
    CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG
    CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA
    GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT
    CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA
    ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT
    GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA
    GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT
    CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG
    GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA
    AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA
    GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG
    GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT
    CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG
    GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG
    AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA
    GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT
    TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT
    CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC TCCAGGTCTC
    CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAATCCAG GCCAACCTGG GCTACCTGGC
    TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGCCGGCCG
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGATCAGCT GGCCCTGAGG GGGAACCGGG ACTTATTGGT
    CCTCCAGGTC CTCCTGGATT ACCTGGTCCT TCAGGACAGA GTATCATAAT TAAAGGAGAT
    GCTGGTCCTC CAGGAATCCC TGGCCAGCCT GGGCTAAAGG GTCTACCAGG ACCCCAAGGA
    CCTCAAGGCT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGTACC
    CGTGGTTTGG ATGGTCCCCC TGGTCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA
    ACCTCCTCTG TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA
    CAATGCCCAC AGGGAACACT TCAGGTCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CCCACGGTCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC
    TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC
    GCAGTTCACA GTCAGACGAT CCAGATTCCC CATTGTCCTC AGGGATGGGA TTCTCTGTGG
    ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA
    GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG
    AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG
    TCAGACATGT TCAGTAAACC TCAGTCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGA
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_203699.1
    CDS289..5364
    Translation

    Target ORF information:

    RefSeq Version NM_033380.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033380.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC
    AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT
    ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC
    CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA
    CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC
    ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA
    TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA
    CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT
    GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA
    TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT
    TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT
    GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG
    GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG
    GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC
    CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA
    GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG
    CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG
    CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA
    GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT
    CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA
    ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT
    GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA
    GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT
    CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG
    GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA
    AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA
    GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG
    GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT
    CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG
    GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG
    AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA
    GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT
    TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT
    CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC TCCAGGTCTC
    CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAATCCAG GCCAACCTGG GCTACCTGGC
    TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGCCGGCCG
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGATCAGCT GGCCCTGAGG GGGAACCGGG ACTTATTGGT
    CCTCCAGGTC CTCCTGGATT ACCTGGTCCT TCAGGACAGA GTATCATAAT TAAAGGAGAT
    GCTGGTCCTC CAGGAATCCC TGGCCAGCCT GGGCTAAAGG GTCTACCAGG ACCCCAAGGA
    CCTCAAGGCT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGTACC
    CGTGGTTTGG ATGGTCCCCC TGGTCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA
    ACCTCCTCTG TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA
    CAATGCCCAC AGGGAACACT TCAGGTCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CCCACGGTCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC
    TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC
    GCAGTTCACA GTCAGACGAT CCAGATTCCC CATTGTCCTC AGGGATGGGA TTCTCTGTGG
    ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA
    GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG
    AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG
    TCAGACATGT TCAGTAAACC TCAGTCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGA
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16784
    Clone ID Related Accession (Same CDS sequence) NM_000495.4 , NM_000495.5
    Accession Version NM_000495.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461056.1, BC151846.1, BC035387.1, M31115.1 and BU688037.1. On Dec 30, 2011 this sequence version replaced NM_000495.3. Transcript Variant: This variant (1) lacks an in-frame segment in the coding region, compared to variant 2. The resulting isoform (1) lacks two Gly-X-Y repeats, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.97910.1, SRR1803617.107038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC
    AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT
    ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC
    CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA
    CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC
    ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA
    TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA
    CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT
    GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA
    TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT
    TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT
    GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG
    GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG
    GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC
    CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA
    GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG
    CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG
    CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA
    GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT
    CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA
    ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT
    GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA
    GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT
    CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG
    GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA
    AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA
    GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG
    GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT
    CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG
    GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG
    AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA
    GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT
    TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT
    CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT
    GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCTCCAGGTC TCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA AGGGAAATCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA
    GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGCCGGC CGGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGATCAG CTGGCCCTGA GGGGGAACCG GGACTTATTG GTCCTCCAGG TCCTCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATCATA ATTAAAGGAG ATGCTGGTCC TCCAGGAATC
    CCTGGCCAGC CTGGGCTAAA GGGTCTACCA GGACCCCAAG GACCTCAAGG CTTACCAGGT
    CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGTA CCCGTGGTTT GGATGGTCCC
    CCTGGTCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TGTTGCACAT
    GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC ACAGGGAACA
    CTTCAGGTCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCACGGT
    CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT
    ACCCCAGAGC CCATGCCAAT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG
    ATCCAGATTC CCCATTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG
    ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCCCC TGGTTCCTGC
    TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC
    TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA
    CCTCAGTCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000486.1
    CDS283..5340
    Translation

    Target ORF information:

    RefSeq Version NM_000495.4
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000495.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC
    AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT
    ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC
    CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA
    CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC
    ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA
    TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA
    CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT
    GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA
    TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT
    TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT
    GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG
    GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG
    GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC
    CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA
    GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG
    CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG
    CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA
    GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT
    CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA
    ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT
    GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA
    GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT
    CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG
    GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA
    AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA
    GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG
    GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT
    CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG
    GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG
    AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA
    GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT
    TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT
    CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT
    GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCTCCAGGTC TCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA AGGGAAATCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA
    GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGCCGGC CGGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGATCAG CTGGCCCTGA GGGGGAACCG GGACTTATTG GTCCTCCAGG TCCTCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATCATA ATTAAAGGAG ATGCTGGTCC TCCAGGAATC
    CCTGGCCAGC CTGGGCTAAA GGGTCTACCA GGACCCCAAG GACCTCAAGG CTTACCAGGT
    CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGTA CCCGTGGTTT GGATGGTCCC
    CCTGGTCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TGTTGCACAT
    GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC ACAGGGAACA
    CTTCAGGTCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCACGGT
    CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT
    ACCCCAGAGC CCATGCCAAT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG
    ATCCAGATTC CCCATTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG
    ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCCCC TGGTTCCTGC
    TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC
    TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA
    CCTCAGTCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16784
    Clone ID Related Accession (Same CDS sequence) NM_000495.4 , NM_000495.5
    Accession Version NM_000495.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, DA461056.1, BC151846.1, BC035387.1, M31115.1 and BU688037.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000495.4. Transcript Variant: This variant (1) lacks an in-frame segment in the coding region, compared to variant 2. The resulting isoform (1) lacks two Gly-X-Y repeats, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.198342.1, SRR1803617.107038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC
    AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT
    ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC
    CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA
    CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC
    ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA
    TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA
    CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT
    GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA
    TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT
    TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT
    GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG
    GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG
    GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC
    CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA
    GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG
    CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG
    CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA
    GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT
    CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA
    ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT
    GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA
    GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT
    CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG
    GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA
    AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA
    GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG
    GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT
    CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG
    GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG
    AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA
    GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT
    TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT
    CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT
    GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCTCCAGGTC TCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA AGGGAAATCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA
    GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGCCGGC CGGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGATCAG CTGGCCCTGA GGGGGAACCG GGACTTATTG GTCCTCCAGG TCCTCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATCATA ATTAAAGGAG ATGCTGGTCC TCCAGGAATC
    CCTGGCCAGC CTGGGCTAAA GGGTCTACCA GGACCCCAAG GACCTCAAGG CTTACCAGGT
    CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGTA CCCGTGGTTT GGATGGTCCC
    CCTGGTCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TGTTGCACAT
    GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC ACAGGGAACA
    CTTCAGGTCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCACGGT
    CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT
    ACCCCAGAGC CCATGCCAAT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG
    ATCCAGATTC CCCATTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG
    ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCCCC TGGTTCCTGC
    TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC
    TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA
    CCTCAGTCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000486.1
    CDS289..5346
    Translation

    Target ORF information:

    RefSeq Version NM_000495.5
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000495.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
    GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC
    AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT
    ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT
    ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA
    ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC
    CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA
    CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC
    ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT
    CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA
    TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA
    CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT
    GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA
    TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT
    TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT
    GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA
    GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG
    GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG
    GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA
    GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC
    CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA
    GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG
    CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG
    CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA
    GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT
    GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT
    CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA
    ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT
    GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA
    GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT
    CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG
    GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA
    AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA
    GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG
    GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA
    CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT
    CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG
    GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG
    AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA
    GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT
    TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT
    CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT
    GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCTCCAGGTC TCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA AGGGAAATCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA
    GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGCCGGC CGGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGATCAG CTGGCCCTGA GGGGGAACCG GGACTTATTG GTCCTCCAGG TCCTCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATCATA ATTAAAGGAG ATGCTGGTCC TCCAGGAATC
    CCTGGCCAGC CTGGGCTAAA GGGTCTACCA GGACCCCAAG GACCTCAAGG CTTACCAGGT
    CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGTA CCCGTGGTTT GGATGGTCCC
    CCTGGTCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TGTTGCACAT
    GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC ACAGGGAACA
    CTTCAGGTCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCACGGT
    CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT
    ACCCCAGAGC CCATGCCAAT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG
    ATCCAGATTC CCCATTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG
    ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCCCC TGGTTCCTGC
    TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC
    TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA
    CCTCAGTCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99143
    Clone ID Related Accession (Same CDS sequence) XM_011530849.2
    Accession Version XM_011530849.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA
    GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA
    CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT
    AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA
    GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA
    CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC
    ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA
    CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA
    CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA
    GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC
    CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG
    ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC
    CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC
    CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT
    GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG
    CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT
    CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA
    TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC
    TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC
    ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG
    GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA
    GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011529151.2
    CDS16..5106
    Translation

    Target ORF information:

    RefSeq Version XM_011530849.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530849.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA
    GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA
    CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT
    AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA
    GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA
    CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC
    ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA
    CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA
    CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA
    GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC
    CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG
    ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC
    CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC
    CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT
    GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG
    CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT
    CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA
    TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC
    TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC
    ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG
    GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA
    GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99144
    Clone ID Related Accession (Same CDS sequence) XM_017029259.2 , XM_017029259.1
    Accession Version XM_017029259.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAGGTCTAC CAGGTCCAGA AGGTCCTCCA
    GGTCTCCCTG GAAATGGAGG TATTAAAGGA GAGAAGGGAA ATCCAGGCCA ACCTGGGCTA
    CCTGGCTTGC CTGGTTTGAA AGGAGATCAA GGACCACCAG GACTCCAGGG TAATCCTGGC
    CGGCCGGGTC TCAATGGAAT GAAAGGAGAT CCTGGTCTCC CTGGTGTTCC AGGATTCCCA
    GGCATGAAAG GACCCAGTGG AGTACCTGGA TCAGCTGGCC CTGAGGGGGA ACCGGGACTT
    ATTGGTCCTC CAGGTCCTCC TGGATTACCT GGTCCTTCAG GACAGAGTAT CATAATTAAA
    GGAGATGCTG GTCCTCCAGG AATCCCTGGC CAGCCTGGGC TAAAGGGTCT ACCAGGACCC
    CAAGGACCTC AAGGCTTACC AGGTCCAACT GGCCCTCCAG GAGATCCTGG ACGCAATGGA
    CTCCCTGGCT TTGATGGTGC AGGAGGGCGC AAAGGAGACC CAGGTCTGCC AGGACAGCCA
    GGTACCCGTG GTTTGGATGG TCCCCCTGGT CCAGATGGAT TGCAAGGTCC CCCAGGTCCC
    CCTGGAACCT CCTCTGTTGC ACATGGATTT CTTATTACAC GCCACAGCCA GACAACGGAT
    GCACCACAAT GCCCACAGGG AACACTTCAG GTCTATGAAG GCTTTTCTCT CCTGTATGTA
    CAAGGAAATA AAAGAGCCCA CGGTCAAGAC TTGGGGACGG CTGGCAGCTG CCTTCGTCGC
    TTTAGTACCA TGCCTTTCAT GTTCTGCAAC ATCAATAATG TTTGCAACTT TGCTTCAAGA
    AATGACTATT CTTACTGGCT CTCTACCCCA GAGCCCATGC CAATGAGCAT GCAACCCCTA
    AAGGGCCAGA GCATCCAGCC ATTCATTAGT CGATGTGCAG TATGTGAAGC TCCAGCTGTG
    GTGATCGCAG TTCACAGTCA GACGATCCAG ATTCCCCATT GTCCTCAGGG ATGGGATTCT
    CTGTGGATTG GTTATTCCTT CATGATGCAT ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA
    GCCCTAGCCT CCCCTGGTTC CTGCTTGGAA GAGTTTCGTT CAGCTCCCTT CATCGAATGT
    CATGGGAGGG GTACCTGTAA CTACTATGCC AACTCCTACA GCTTTTGGCT GGCAACTGTA
    GATGTGTCAG ACATGTTCAG TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG
    ACACGAATTA GCCGATGTCA AGTGTGCATG AAGAGGACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884748.1
    CDS16..5097
    Translation

    Target ORF information:

    RefSeq Version XM_017029259.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029259.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAGGTCTAC CAGGTCCAGA AGGTCCTCCA
    GGTCTCCCTG GAAATGGAGG TATTAAAGGA GAGAAGGGAA ATCCAGGCCA ACCTGGGCTA
    CCTGGCTTGC CTGGTTTGAA AGGAGATCAA GGACCACCAG GACTCCAGGG TAATCCTGGC
    CGGCCGGGTC TCAATGGAAT GAAAGGAGAT CCTGGTCTCC CTGGTGTTCC AGGATTCCCA
    GGCATGAAAG GACCCAGTGG AGTACCTGGA TCAGCTGGCC CTGAGGGGGA ACCGGGACTT
    ATTGGTCCTC CAGGTCCTCC TGGATTACCT GGTCCTTCAG GACAGAGTAT CATAATTAAA
    GGAGATGCTG GTCCTCCAGG AATCCCTGGC CAGCCTGGGC TAAAGGGTCT ACCAGGACCC
    CAAGGACCTC AAGGCTTACC AGGTCCAACT GGCCCTCCAG GAGATCCTGG ACGCAATGGA
    CTCCCTGGCT TTGATGGTGC AGGAGGGCGC AAAGGAGACC CAGGTCTGCC AGGACAGCCA
    GGTACCCGTG GTTTGGATGG TCCCCCTGGT CCAGATGGAT TGCAAGGTCC CCCAGGTCCC
    CCTGGAACCT CCTCTGTTGC ACATGGATTT CTTATTACAC GCCACAGCCA GACAACGGAT
    GCACCACAAT GCCCACAGGG AACACTTCAG GTCTATGAAG GCTTTTCTCT CCTGTATGTA
    CAAGGAAATA AAAGAGCCCA CGGTCAAGAC TTGGGGACGG CTGGCAGCTG CCTTCGTCGC
    TTTAGTACCA TGCCTTTCAT GTTCTGCAAC ATCAATAATG TTTGCAACTT TGCTTCAAGA
    AATGACTATT CTTACTGGCT CTCTACCCCA GAGCCCATGC CAATGAGCAT GCAACCCCTA
    AAGGGCCAGA GCATCCAGCC ATTCATTAGT CGATGTGCAG TATGTGAAGC TCCAGCTGTG
    GTGATCGCAG TTCACAGTCA GACGATCCAG ATTCCCCATT GTCCTCAGGG ATGGGATTCT
    CTGTGGATTG GTTATTCCTT CATGATGCAT ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA
    GCCCTAGCCT CCCCTGGTTC CTGCTTGGAA GAGTTTCGTT CAGCTCCCTT CATCGAATGT
    CATGGGAGGG GTACCTGTAA CTACTATGCC AACTCCTACA GCTTTTGGCT GGCAACTGTA
    GATGTGTCAG ACATGTTCAG TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG
    ACACGAATTA GCCGATGTCA AGTGTGCATG AAGAGGACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99144
    Clone ID Related Accession (Same CDS sequence) XM_017029259.2 , XM_017029259.1
    Accession Version XM_017029259.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAGGTCTAC CAGGTCCAGA AGGTCCTCCA
    GGTCTCCCTG GAAATGGAGG TATTAAAGGA GAGAAGGGAA ATCCAGGCCA ACCTGGGCTA
    CCTGGCTTGC CTGGTTTGAA AGGAGATCAA GGACCACCAG GACTCCAGGG TAATCCTGGC
    CGGCCGGGTC TCAATGGAAT GAAAGGAGAT CCTGGTCTCC CTGGTGTTCC AGGATTCCCA
    GGCATGAAAG GACCCAGTGG AGTACCTGGA TCAGCTGGCC CTGAGGGGGA ACCGGGACTT
    ATTGGTCCTC CAGGTCCTCC TGGATTACCT GGTCCTTCAG GACAGAGTAT CATAATTAAA
    GGAGATGCTG GTCCTCCAGG AATCCCTGGC CAGCCTGGGC TAAAGGGTCT ACCAGGACCC
    CAAGGACCTC AAGGCTTACC AGGTCCAACT GGCCCTCCAG GAGATCCTGG ACGCAATGGA
    CTCCCTGGCT TTGATGGTGC AGGAGGGCGC AAAGGAGACC CAGGTCTGCC AGGACAGCCA
    GGTACCCGTG GTTTGGATGG TCCCCCTGGT CCAGATGGAT TGCAAGGTCC CCCAGGTCCC
    CCTGGAACCT CCTCTGTTGC ACATGGATTT CTTATTACAC GCCACAGCCA GACAACGGAT
    GCACCACAAT GCCCACAGGG AACACTTCAG GTCTATGAAG GCTTTTCTCT CCTGTATGTA
    CAAGGAAATA AAAGAGCCCA CGGTCAAGAC TTGGGGACGG CTGGCAGCTG CCTTCGTCGC
    TTTAGTACCA TGCCTTTCAT GTTCTGCAAC ATCAATAATG TTTGCAACTT TGCTTCAAGA
    AATGACTATT CTTACTGGCT CTCTACCCCA GAGCCCATGC CAATGAGCAT GCAACCCCTA
    AAGGGCCAGA GCATCCAGCC ATTCATTAGT CGATGTGCAG TATGTGAAGC TCCAGCTGTG
    GTGATCGCAG TTCACAGTCA GACGATCCAG ATTCCCCATT GTCCTCAGGG ATGGGATTCT
    CTGTGGATTG GTTATTCCTT CATGATGCAT ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA
    GCCCTAGCCT CCCCTGGTTC CTGCTTGGAA GAGTTTCGTT CAGCTCCCTT CATCGAATGT
    CATGGGAGGG GTACCTGTAA CTACTATGCC AACTCCTACA GCTTTTGGCT GGCAACTGTA
    GATGTGTCAG ACATGTTCAG TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG
    ACACGAATTA GCCGATGTCA AGTGTGCATG AAGAGGACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884748.1
    CDS16..5097
    Translation

    Target ORF information:

    RefSeq Version XM_017029259.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029259.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAGGTCTAC CAGGTCCAGA AGGTCCTCCA
    GGTCTCCCTG GAAATGGAGG TATTAAAGGA GAGAAGGGAA ATCCAGGCCA ACCTGGGCTA
    CCTGGCTTGC CTGGTTTGAA AGGAGATCAA GGACCACCAG GACTCCAGGG TAATCCTGGC
    CGGCCGGGTC TCAATGGAAT GAAAGGAGAT CCTGGTCTCC CTGGTGTTCC AGGATTCCCA
    GGCATGAAAG GACCCAGTGG AGTACCTGGA TCAGCTGGCC CTGAGGGGGA ACCGGGACTT
    ATTGGTCCTC CAGGTCCTCC TGGATTACCT GGTCCTTCAG GACAGAGTAT CATAATTAAA
    GGAGATGCTG GTCCTCCAGG AATCCCTGGC CAGCCTGGGC TAAAGGGTCT ACCAGGACCC
    CAAGGACCTC AAGGCTTACC AGGTCCAACT GGCCCTCCAG GAGATCCTGG ACGCAATGGA
    CTCCCTGGCT TTGATGGTGC AGGAGGGCGC AAAGGAGACC CAGGTCTGCC AGGACAGCCA
    GGTACCCGTG GTTTGGATGG TCCCCCTGGT CCAGATGGAT TGCAAGGTCC CCCAGGTCCC
    CCTGGAACCT CCTCTGTTGC ACATGGATTT CTTATTACAC GCCACAGCCA GACAACGGAT
    GCACCACAAT GCCCACAGGG AACACTTCAG GTCTATGAAG GCTTTTCTCT CCTGTATGTA
    CAAGGAAATA AAAGAGCCCA CGGTCAAGAC TTGGGGACGG CTGGCAGCTG CCTTCGTCGC
    TTTAGTACCA TGCCTTTCAT GTTCTGCAAC ATCAATAATG TTTGCAACTT TGCTTCAAGA
    AATGACTATT CTTACTGGCT CTCTACCCCA GAGCCCATGC CAATGAGCAT GCAACCCCTA
    AAGGGCCAGA GCATCCAGCC ATTCATTAGT CGATGTGCAG TATGTGAAGC TCCAGCTGTG
    GTGATCGCAG TTCACAGTCA GACGATCCAG ATTCCCCATT GTCCTCAGGG ATGGGATTCT
    CTGTGGATTG GTTATTCCTT CATGATGCAT ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA
    GCCCTAGCCT CCCCTGGTTC CTGCTTGGAA GAGTTTCGTT CAGCTCCCTT CATCGAATGT
    CATGGGAGGG GTACCTGTAA CTACTATGCC AACTCCTACA GCTTTTGGCT GGCAACTGTA
    GATGTGTCAG ACATGTTCAG TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG
    ACACGAATTA GCCGATGTCA AGTGTGCATG AAGAGGACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99145
    Clone ID Related Accession (Same CDS sequence) XM_017029260.1
    Accession Version XM_017029260.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCTA CCAGGTCCAG AAGGTCCTCC AGGTCTCCCT
    GGAAATGGAG GTATTAAAGG AGAGAAGGGA AATCCAGGCC AACCTGGGCT ACCTGGCTTG
    CCTGGTTTGA AAGGAGATCA AGGACCACCA GGACTCCAGG GTAATCCTGG CCGGCCGGGT
    CTCAATGGAA TGAAAGGAGA TCCTGGTCTC CCTGGTGTTC CAGGATTCCC AGGCATGAAA
    GGACCCAGTG GAGTACCTGG ATCAGCTGGC CCTGAGGGGG AACCGGGACT TATTGGTCCT
    CCAGGTCCTC CTGGATTACC TGGTCCTTCA GGACAGAGTA TCATAATTAA AGGAGATGCT
    GGTCCTCCAG GAATCCCTGG CCAGCCTGGG CTAAAGGGTC TACCAGGACC CCAAGGACCT
    CAAGGCTTAC CAGGTCCAAC TGGCCCTCCA GGAGATCCTG GACGCAATGG ACTCCCTGGC
    TTTGATGGTG CAGGAGGGCG CAAAGGAGAC CCAGGTCTGC CAGGACAGCC AGGTACCCGT
    GGTTTGGATG GTCCCCCTGG TCCAGATGGA TTGCAAGGTC CCCCAGGTCC CCCTGGAACC
    TCCTCTGTTG CACATGGATT TCTTATTACA CGCCACAGCC AGACAACGGA TGCACCACAA
    TGCCCACAGG GAACACTTCA GGTCTATGAA GGCTTTTCTC TCCTGTATGT ACAAGGAAAT
    AAAAGAGCCC ACGGTCAAGA CTTGGGGACG GCTGGCAGCT GCCTTCGTCG CTTTAGTACC
    ATGCCTTTCA TGTTCTGCAA CATCAATAAT GTTTGCAACT TTGCTTCAAG AAATGACTAT
    TCTTACTGGC TCTCTACCCC AGAGCCCATG CCAATGAGCA TGCAACCCCT AAAGGGCCAG
    AGCATCCAGC CATTCATTAG TCGATGTGCA GTATGTGAAG CTCCAGCTGT GGTGATCGCA
    GTTCACAGTC AGACGATCCA GATTCCCCAT TGTCCTCAGG GATGGGATTC TCTGTGGATT
    GGTTATTCCT TCATGATGCA TACAAGTGCA GGGGCAGAAG GCTCAGGTCA AGCCCTAGCC
    TCCCCTGGTT CCTGCTTGGA AGAGTTTCGT TCAGCTCCCT TCATCGAATG TCATGGGAGG
    GGTACCTGTA ACTACTATGC CAACTCCTAC AGCTTTTGGC TGGCAACTGT AGATGTGTCA
    GACATGTTCA GTAAACCTCA GTCAGAAACG CTGAAAGCAG GAGACTTGAG GACACGAATT
    AGCCGATGTC AAGTGTGCAT GAAGAGGACA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884749.1
    CDS16..5088
    Translation

    Target ORF information:

    RefSeq Version XM_017029260.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029260.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCTA CCAGGTCCAG AAGGTCCTCC AGGTCTCCCT
    GGAAATGGAG GTATTAAAGG AGAGAAGGGA AATCCAGGCC AACCTGGGCT ACCTGGCTTG
    CCTGGTTTGA AAGGAGATCA AGGACCACCA GGACTCCAGG GTAATCCTGG CCGGCCGGGT
    CTCAATGGAA TGAAAGGAGA TCCTGGTCTC CCTGGTGTTC CAGGATTCCC AGGCATGAAA
    GGACCCAGTG GAGTACCTGG ATCAGCTGGC CCTGAGGGGG AACCGGGACT TATTGGTCCT
    CCAGGTCCTC CTGGATTACC TGGTCCTTCA GGACAGAGTA TCATAATTAA AGGAGATGCT
    GGTCCTCCAG GAATCCCTGG CCAGCCTGGG CTAAAGGGTC TACCAGGACC CCAAGGACCT
    CAAGGCTTAC CAGGTCCAAC TGGCCCTCCA GGAGATCCTG GACGCAATGG ACTCCCTGGC
    TTTGATGGTG CAGGAGGGCG CAAAGGAGAC CCAGGTCTGC CAGGACAGCC AGGTACCCGT
    GGTTTGGATG GTCCCCCTGG TCCAGATGGA TTGCAAGGTC CCCCAGGTCC CCCTGGAACC
    TCCTCTGTTG CACATGGATT TCTTATTACA CGCCACAGCC AGACAACGGA TGCACCACAA
    TGCCCACAGG GAACACTTCA GGTCTATGAA GGCTTTTCTC TCCTGTATGT ACAAGGAAAT
    AAAAGAGCCC ACGGTCAAGA CTTGGGGACG GCTGGCAGCT GCCTTCGTCG CTTTAGTACC
    ATGCCTTTCA TGTTCTGCAA CATCAATAAT GTTTGCAACT TTGCTTCAAG AAATGACTAT
    TCTTACTGGC TCTCTACCCC AGAGCCCATG CCAATGAGCA TGCAACCCCT AAAGGGCCAG
    AGCATCCAGC CATTCATTAG TCGATGTGCA GTATGTGAAG CTCCAGCTGT GGTGATCGCA
    GTTCACAGTC AGACGATCCA GATTCCCCAT TGTCCTCAGG GATGGGATTC TCTGTGGATT
    GGTTATTCCT TCATGATGCA TACAAGTGCA GGGGCAGAAG GCTCAGGTCA AGCCCTAGCC
    TCCCCTGGTT CCTGCTTGGA AGAGTTTCGT TCAGCTCCCT TCATCGAATG TCATGGGAGG
    GGTACCTGTA ACTACTATGC CAACTCCTAC AGCTTTTGGC TGGCAACTGT AGATGTGTCA
    GACATGTTCA GTAAACCTCA GTCAGAAACG CTGAAAGCAG GAGACTTGAG GACACGAATT
    AGCCGATGTC AAGTGTGCAT GAAGAGGACA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99146
    Clone ID Related Accession (Same CDS sequence) XM_017029261.1
    Accession Version XM_017029261.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA
    GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA
    CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGTGG
    TTTGACTTGA TTGTACAAAG GGAGATCCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884750.1
    CDS16..4008
    Translation

    Target ORF information:

    RefSeq Version XM_017029261.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029261.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA
    GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA
    CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGTGG
    TTTGACTTGA TTGTACAAAG GGAGATCCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99147
    Clone ID Related Accession (Same CDS sequence) XM_017029262.2 , XM_017029262.1
    Accession Version XM_017029262.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAACTATCC CACCTTCACA TTTTGGGTTG
    TCACAAAGTT CTCAATTCTA CTGCTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884751.1
    CDS16..3885
    Translation

    Target ORF information:

    RefSeq Version XM_017029262.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029262.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAACTATCC CACCTTCACA TTTTGGGTTG
    TCACAAAGTT CTCAATTCTA CTGCTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99147
    Clone ID Related Accession (Same CDS sequence) XM_017029262.2 , XM_017029262.1
    Accession Version XM_017029262.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAACTATCC CACCTTCACA TTTTGGGTTG
    TCACAAAGTT CTCAATTCTA CTGCTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884751.1
    CDS16..3885
    Translation

    Target ORF information:

    RefSeq Version XM_017029262.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029262.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
    TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA
    TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG
    GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA
    CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT
    GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC
    CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC
    AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG
    GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT
    CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT
    CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG
    GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG
    GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT
    CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT
    GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC
    AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT
    GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC
    ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA
    GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT
    CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT
    CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA
    ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG
    CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT
    CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT
    GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA
    CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG
    GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA
    GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAACTATCC CACCTTCACA TTTTGGGTTG
    TCACAAAGTT CTCAATTCTA CTGCTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99148
    Clone ID Related Accession (Same CDS sequence) XM_017029263.2 , XM_017029263.1
    Accession Version XM_017029263.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA
    GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA
    CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT
    AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA
    GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA
    CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC
    ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA
    CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA
    CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA
    GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC
    CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG
    ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC
    CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC
    CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT
    GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG
    CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT
    CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA
    TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC
    TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC
    ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG
    GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA
    GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884752.1
    CDS780..4190
    Translation

    Target ORF information:

    RefSeq Version XM_017029263.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029263.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA
    GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA
    CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT
    AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA
    GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA
    CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC
    ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA
    CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA
    CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA
    GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC
    CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG
    ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC
    CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC
    CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT
    GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG
    CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT
    CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA
    TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC
    TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC
    ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG
    GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA
    GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99148
    Clone ID Related Accession (Same CDS sequence) XM_017029263.2 , XM_017029263.1
    Accession Version XM_017029263.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product collagen alpha-5(IV) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA
    GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA
    CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT
    AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA
    GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA
    CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC
    ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA
    CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA
    CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA
    GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC
    CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG
    ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC
    CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC
    CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT
    GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG
    CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT
    CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA
    TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC
    TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC
    ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG
    GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA
    GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884752.1
    CDS774..4184
    Translation

    Target ORF information:

    RefSeq Version XM_017029263.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029263.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
    GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA
    ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG
    AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC
    ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA
    GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT
    GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT
    GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA
    GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA
    GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA
    CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT
    GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT
    GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG
    CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA
    CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT
    CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA
    CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA
    GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG
    AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC
    CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG
    GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT
    GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG
    AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA
    CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA
    GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC
    CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT
    CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT
    ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA
    GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT
    AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT
    CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT
    GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT
    GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT
    CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT
    CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG
    CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA
    GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA
    CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT
    AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA
    GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA
    CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC
    ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA
    CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA
    CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA
    GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC
    CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG
    ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC
    CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC
    CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT
    GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG
    CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT
    CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA
    TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC
    TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC
    ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG
    GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA
    GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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