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CRYAB cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CRYAB
Entrez Gene ID 1410
Full Name crystallin alpha B
Synonyms CMD1II,CRYA2,CTPP2,CTRCT16,HEL-S-101,HSPB5,MFM2
General protein information
Preferred Names

crystallin alpha B

Names

alpha-crystallin B chain
epididymis secretory protein Li 101
heat shock protein beta-5
heat-shock 20 kD like-protein
renal carcinoma antigen NY-REN-27
rosenthal fiber component

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11q23.1

Summary Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].
Disorder MIM:

123590

Disorder Html: Myopathy, cardioskeletal, desmin-related, with cataract, 608810 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001330379.1 NP_001317308.1 alpha-crystallin B chain isoform 2
NM_001289808.1 NP_001276737.1 alpha-crystallin B chain isoform 1
NM_001289807.1 NP_001276736.1 alpha-crystallin B chain isoform 1
NM_001885.2 NP_001876.1 alpha-crystallin B chain isoform 1
XM_011542608.1 XP_011540910.1 alpha-crystallin B chain isoform X1



Homo sapiens (human) CRYAB NP_001876.1
Mus musculus (house mouse) Cryab NP_034094.1
Danio rerio (zebrafish) cryabb NP_001002670.1
Macaca mulatta (rhesus monkey) CRYAB XP_002799808.1
Canis lupus familiaris (dog) CRYAB XP_862258.2
Bos taurus (cattle) CRYAB NP_776715.1
Gallus gallus (chicken) CRYAB NP_990507.1
Pan troglodytes (chimpanzee) CRYAB XP_508752.2
Rattus norvegicus (Norway rat) Cryab NP_037067.1
Drosophila melanogaster (fruit fly) l(2)efl NP_523827.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007162 XP_308606.2
Xenopus tropicalis (tropical clawed frog) cryab XP_002932964.1


Related articles in PubMed

The Human 343delT HSPB5 Chaperone Associated with Early-onset Skeletal Myopathy Causes Defects in Protein Solubility.
Mitzelfelt KA, Limphong P, Choi MJ, Kondrat FD, Lai S, Kolander KD, Kwok WM, Dai Q, Grzybowski MN, Zhang H, Taylor GM, Lui Q, Thao MT, Hudson JA, Barresi R, Bushby K, Jungbluth H, Wraige E, Geurts AM, Benesch JL, Riedel M, Christians ES, Minella AC, Benjamin IJ
The Journal of biological chemistry291(29)14939-53(2016 07)

Inhibition of the Expression of the Small Heat Shock Protein αB-Crystallin Inhibits Exosome Secretion in Human Retinal Pigment Epithelial Cells in Culture.
Gangalum RK, Bhat AM, Kohan SA, Bhat SP
The Journal of biological chemistry291(25)12930-42(2016 Jun)

αB-Crystallin Interacts with Nav1.5 and Regulates Ubiquitination and Internalization of Cell Surface Nav1.5.
Huang Y, Wang Z, Liu Y, Xiong H, Zhao Y, Wu L, Yuan C, Wang L, Hou Y, Yu G, Huang Z, Xu C, Chen Q, Wang QK
The Journal of biological chemistry291(21)11030-41(2016 May)

Structure, Chaperone Activity, and Aggregation of Wild-Type and R12C Mutant αB-Crystallins in the Presence of Thermal Stress and Calcium Ion - Implications for Role of Calcium in Cataract Pathogenesis.
Kashani MR, Yousefi R, Akbarian M, Alavianmehr MM, Ghasemi Y
Biochemistry. Biokhimiia81(2)122-34(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A missense mutation (p.D109G) in CRYAB causes restrictive cardiomyopathy (RCM).
Title: The novel αB-crystallin (CRYAB) mutation p.D109G causes restrictive cardiomyopathy.

Results demonstrated that CRYAA rs7278468 and CRYAB rs370803064/rs387907338 are correlated with the risk and clinicopathological features of children suffering from congenital cataract.
Title: Correlations of single nucleotide polymorphisms of CRYAA and CRYAB genes with the risk and clinicopathological features of children suffering from congenital cataract.

A missense mutation in alpha B-crystallin that changes proline 20 to an arginine leads to diminished anti-apoptotic activity compared with the native protein.
Title: The P20R mutation of αB-crystallin diminishes its anti-apoptotic activity in human lens epithelial cells.

This work examines the molecular mechanism by which two canonical sHsps, alphaB-crystallin (alphaB-c) and Hsp27, interact with aggregation-prone alpha-syn to prevent its aggregation in vitro Both sHsps are very effective inhibitors of alpha-syn aggregation
Title: Small Heat-shock Proteins Prevent α-Synuclein Aggregation via Transient Interactions and Their Efficacy Is Affected by the Rate of Aggregation.

phosphorylation finely regulates the chaperone activity of CRYAB with multipass TMPs and suggest a pivotal role for S59 in this process
Title: Differential phosphorylation-based regulation of αB-crystallin chaperone activity for multipass transmembrane proteins.

The following CRYAB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYAB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu16676 NM_001885.2
Latest version!
Homo sapiens crystallin alpha B (CRYAB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16676 NM_001289808.1
Latest version!
Homo sapiens crystallin alpha B (CRYAB), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16676 NM_001289807.1
Latest version!
Homo sapiens crystallin alpha B (CRYAB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16676 XM_011542608.1
Latest version!
Homo sapiens crystallin alpha B (CRYAB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu57086 NM_001330379.1
Latest version!
Homo sapiens crystallin alpha B (CRYAB), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16676
Clone ID Related Accession (Same CDS sequence) NM_001289808.1 , NM_001289807.1 , NM_001885.2 , XM_011542608.1
Accession Version NM_001289808.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product alpha-crystallin B chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI548180.1, BE076509.1, BM721803.1, BC007008.1 and BF339748.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DT217536.1, BM926107.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
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421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276737.1
CDS303..830
Misc Feature(1)246..248
Misc Feature(2)303..455
Misc Feature(3)303..305
Misc Feature(4)333..785
Misc Feature(5)357..359
Misc Feature(6)435..437
Misc Feature(7)444..446
Misc Feature(8)477..479
Misc Feature(9)480..482
Misc Feature(10)501..752
Misc Feature(11)order(504..518,534..536,540..542,546..551,660..662,723..728)
Misc Feature(12)504..506
Misc Feature(13)576..578
Misc Feature(14)798..800
Exon (1)1..503
Exon (2)504..626
Exon (3)627..974
Translation

Target ORF information:

RefSeq Version NM_001289808.1
Organism Homo sapiens(Human)
Definition Homo sapiens crystallin alpha B (CRYAB), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289808.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16676
Clone ID Related Accession (Same CDS sequence) NM_001289808.1 , NM_001289807.1 , NM_001885.2 , XM_011542608.1
Accession Version NM_001289807.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product alpha-crystallin B chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB506606.1, BI759672.1, BM721803.1, BC007008.1 and BF339748.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI759672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276736.1
CDS274..801
Misc Feature(1)217..219
Misc Feature(2)274..426
Misc Feature(3)274..276
Misc Feature(4)304..756
Misc Feature(5)328..330
Misc Feature(6)406..408
Misc Feature(7)415..417
Misc Feature(8)448..450
Misc Feature(9)451..453
Misc Feature(10)472..723
Misc Feature(11)order(475..489,505..507,511..513,517..522,631..633,694..699)
Misc Feature(12)475..477
Misc Feature(13)547..549
Misc Feature(14)769..771
Exon (1)1..75
Exon (2)76..474
Exon (3)475..597
Exon (4)598..945
Translation

Target ORF information:

RefSeq Version NM_001289807.1
Organism Homo sapiens(Human)
Definition Homo sapiens crystallin alpha B (CRYAB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289807.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16676
Clone ID Related Accession (Same CDS sequence) NM_001289808.1 , NM_001289807.1 , NM_001885.2 , XM_011542608.1
Accession Version NM_001885.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product alpha-crystallin B chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB506606.1, BM721803.1, BC007008.1 and BF339748.1. This sequence is a reference standard in the RefSeqGene project. On Jan 31, 2014 this sequence version replaced NM_001885.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG750124.1, CF127116.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001876.1
CDS308..835
Misc Feature(1)251..253
Misc Feature(2)308..460
Misc Feature(3)308..310
Misc Feature(4)338..790
Misc Feature(5)362..364
Misc Feature(6)362..364
Misc Feature(7)368..370
Misc Feature(8)371..373
Misc Feature(9)434..436
Misc Feature(10)440..442
Misc Feature(11)440..442
Misc Feature(12)449..451
Misc Feature(13)455..457
Misc Feature(14)464..466
Misc Feature(15)482..484
Misc Feature(16)482..484
Misc Feature(17)485..487
Misc Feature(18)506..757
Misc Feature(19)order(509..523,539..541,545..547,551..556,665..667,728..733)
Misc Feature(20)509..511
Misc Feature(21)533..535
Misc Feature(22)551..553
Misc Feature(23)581..583
Misc Feature(24)581..583
Misc Feature(25)803..805
Misc Feature(26)803..805
Misc Feature(27)815..817
Misc Feature(28)827..829
Misc Feature(29)830..832
Exon (1)1..109
Exon (2)110..508
Exon (3)509..631
Exon (4)632..979
Translation

Target ORF information:

RefSeq Version NM_001885.2
Organism Homo sapiens(Human)
Definition Homo sapiens crystallin alpha B (CRYAB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001885.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16676
Clone ID Related Accession (Same CDS sequence) NM_001289808.1 , NM_001289807.1 , NM_001885.2 , XM_011542608.1
Accession Version XM_011542608.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product alpha-crystallin B chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011540910.1
CDS289..816
Misc Feature(1)289..441
Misc Feature(2)319..771
Misc Feature(3)487..738
Misc Feature(4)order(490..504,520..522,526..528,532..537,646..648,709..714)
Translation

Target ORF information:

RefSeq Version XM_011542608.1
Organism Homo sapiens(Human)
Definition Homo sapiens crystallin alpha B (CRYAB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011542608.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57086
Clone ID Related Accession (Same CDS sequence) NM_001330379.1
Accession Version NM_001330379.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 327bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product alpha-crystallin B chain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000907.6, BQ437433.1 and BF339748.1. On Aug 29, 2016 this sequence version replaced XM_011542609.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ709037.1, BQ437433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMN03267751 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGCGCCTGG AGAAGGACAG GTTCTCTGTC AACCTGGATG TGAAGCACTT CTCCCCAGAG 
GAACTCAAAG TTAAGGTGTT GGGAGATGTG ATTGAGGTGC ATGGAAAACA TGAAGAGCGC
CAGGATGAAC ATGGTTTCAT CTCCAGGGAG TTCCACAGGA AATACCGGAT CCCAGCTGAT
GTAGACCCTC TCACCATTAC TTCATCCCTG TCATCTGATG GGGTCCTCAC TGTGAATGGA
CCAAGGAAAC AGGTCTCTGG CCCTGAGCGC ACCATTCCCA TCACCCGTGA AGAGAAGCCT
GCTGTCACCG CAGCCCCCAA GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317308.1
CDS84..410
Misc Feature(1)60..62
Exon (1)1..83
Exon (2)84..206
Exon (3)207..554
Translation

Target ORF information:

RefSeq Version NM_001330379.1
Organism Homo sapiens(Human)
Definition Homo sapiens crystallin alpha B (CRYAB), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001330379.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGCGCCTGG AGAAGGACAG GTTCTCTGTC AACCTGGATG TGAAGCACTT CTCCCCAGAG 
GAACTCAAAG TTAAGGTGTT GGGAGATGTG ATTGAGGTGC ATGGAAAACA TGAAGAGCGC
CAGGATGAAC ATGGTTTCAT CTCCAGGGAG TTCCACAGGA AATACCGGAT CCCAGCTGAT
GTAGACCCTC TCACCATTAC TTCATCCCTG TCATCTGATG GGGTCCTCAC TGTGAATGGA
CCAAGGAAAC AGGTCTCTGG CCCTGAGCGC ACCATTCCCA TCACCCGTGA AGAGAAGCCT
GCTGTCACCG CAGCCCCCAA GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The Human 343delT HSPB5 Chaperone Associated with Early-onset Skeletal Myopathy Causes Defects in Protein Solubility.
The Journal of biological chemistry291(29)14939-53(2016 07)
Mitzelfelt KA,Limphong P,Choi MJ,Kondrat FD,Lai S,Kolander KD,Kwok WM,Dai Q,Grzybowski MN,Zhang H,Taylor GM,Lui Q,Thao MT,Hudson JA,Barresi R,Bushby K,Jungbluth H,Wraige E,Geurts AM,Benesch JL,Riedel M,Christians ES,Minella AC,Benjamin IJ


book

Inhibition of the Expression of the Small Heat Shock Protein αB-Crystallin Inhibits Exosome Secretion in Human Retinal Pigment Epithelial Cells in Culture.
The Journal of biological chemistry291(25)12930-42(2016 Jun)
Gangalum RK,Bhat AM,Kohan SA,Bhat SP


book

αB-Crystallin Interacts with Nav1.5 and Regulates Ubiquitination and Internalization of Cell Surface Nav1.5.
The Journal of biological chemistry291(21)11030-41(2016 May)
Huang Y,Wang Z,Liu Y,Xiong H,Zhao Y,Wu L,Yuan C,Wang L,Hou Y,Yu G,Huang Z,Xu C,Chen Q,Wang QK


book

Structure, Chaperone Activity, and Aggregation of Wild-Type and R12C Mutant αB-Crystallins in the Presence of Thermal Stress and Calcium Ion - Implications for Role of Calcium in Cataract Pathogenesis.
Biochemistry. Biokhimiia81(2)122-34(2016 Feb)
Kashani MR,Yousefi R,Akbarian M,Alavianmehr MM,Ghasemi Y


 
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