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CYBA cDNA ORF clone, Homo sapiens (human)

Gene Symbol CYBA
Entrez Gene ID 1535
Full Name cytochrome b-245, alpha polypeptide
Synonyms p22-PHOX
General protein information
Preferred Names
cytochrome b-245 light chain
Names
cytochrome b-245 light chain
p22phox
cytochrome b light chain
p22 phagocyte B-cytochrome
cytochrome b(558) alpha chain
cytochrome b558 subunit alpha
cytochrome b(558) alpha-subunit
cytochrome b, alpha polypeptide
flavocytochrome b-558 alpha polypeptide
neutrophil cytochrome b 22 kDa polypeptide
superoxide-generating NADPH oxidase light chain subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q24

Summary Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

608508

Disorder Html: Chronic granulomatous disease, autosomal, due to deficiency of CYBA,

mRNA and Protein(s)

mRNA Protein Name
XM_011522905 XP_011521207 cytochrome b-245 light chain isoform X1
NM_000101 NP_000092 cytochrome b-245 light chain



Homo sapiens (human) CYBA NP_000092.2
Pan troglodytes (chimpanzee) CYBA XP_523459.1
Macaca mulatta (Rhesus monkey) LOC696748 XP_001089060.1
Canis lupus familiaris (dog) CYBA NP_001093760.1
Bos taurus (cattle) CYBA NP_776459.1
Mus musculus (house mouse) Cyba NP_031832.2
Rattus norvegicus (Norway rat) Cyba NP_077074.1
Danio rerio (zebrafish) cyba NP_956873.1
Xenopus (Silurana) tropicalis (western clawed frog) cyba NP_001016480.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CYBA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYBA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu57139 XM_011522905 PREDICTED: Homo sapiens cytochrome b-245, alpha polypeptide (CYBA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu21427 NM_000101 Homo sapiens cytochrome b-245, alpha polypeptide (CYBA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu57139
Clone ID Related Accession (Same CDS sequence) XM_011522905
Accession Version XM_011522905.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cytochrome b-245 light chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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361
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481
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601
ATGGGGCAGA TCGAGTGGGC CATGTGGGCC AACGAACAGG CGCTGGCGTC CGGCCTGATC 
CTCATCACCG GGGGCATCGT GGCCACAGCT GGGCGCTTCA CCCAGTGGTA CTTTGGTGCC
TACTCCATTG TGGCGGGCGT GTTTGTGTGC CTGCTGGAGT ACCCCCGGGG GAAGAGGAAG
AAGGGCTCCA CCATGGAGCG CTGGGGACAG AAGTACATGA CCGCCGTGGT GAAGCTGTTC
GGGCCCTTTA CCAGGAATTA CTATGTTCGG GCCGTCCTGC ATCTCCTGCT CTCGGTGCCC
GCCGGCTTCC TGCTGGCCAC CATCCTTGGG ACCGCCTGCC TGGCCATTGC GAGCGGCATC
TACCTACTGA GCCAAGTCCC TCCTGCCCGC AGAGGCAACT GCCCTCCCCT TGCTGTGCGT
CTTCTAGTGT GTGTGCGTGG GAAAGGTGCA GCACTTTCTG TGTACGCCTT CCGCGTATGG
AAGTGGAGTT CTGTTTCAAG CCTCACTGAC CTGCCACCGG CAGAACTCTC CTTTAGAACC
CCTTCCTGTT GCCAAGTGCT CATCTGGGCT GTTGCTTTGT TTTCCGGGGG CCACCCGCAT
CCCCCCCGTC TCCCTGCTGC CACGCACAGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521207.1
CDS73..705
Misc Feature(1)76..>450(+)
Translation

Target ORF information:

RefSeq Version XM_011522905
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome b-245, alpha polypeptide (CYBA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522905

ORF Insert Sequence:

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121
181
241
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361
421
481
541
601
ATGGGGCAGA TCGAGTGGGC CATGTGGGCC AACGAACAGG CGCTGGCGTC CGGCCTGATC 
CTCATCACCG GGGGCATCGT GGCCACAGCT GGGCGCTTCA CCCAGTGGTA CTTTGGTGCC
TACTCCATTG TGGCGGGCGT GTTTGTGTGC CTGCTGGAGT ACCCCCGGGG GAAGAGGAAG
AAGGGCTCCA CCATGGAGCG CTGGGGACAG AAGTACATGA CCGCCGTGGT GAAGCTGTTC
GGGCCCTTTA CCAGGAATTA CTATGTTCGG GCCGTCCTGC ATCTCCTGCT CTCGGTGCCC
GCCGGCTTCC TGCTGGCCAC CATCCTTGGG ACCGCCTGCC TGGCCATTGC GAGCGGCATC
TACCTACTGA GCCAAGTCCC TCCTGCCCGC AGAGGCAACT GCCCTCCCCT TGCTGTGCGT
CTTCTAGTGT GTGTGCGTGG GAAAGGTGCA GCACTTTCTG TGTACGCCTT CCGCGTATGG
AAGTGGAGTT CTGTTTCAAG CCTCACTGAC CTGCCACCGG CAGAACTCTC CTTTAGAACC
CCTTCCTGTT GCCAAGTGCT CATCTGGGCT GTTGCTTTGT TTTCCGGGGG CCACCCGCAT
CCCCCCCGTC TCCCTGCTGC CACGCACAGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21427
Clone ID Related Accession (Same CDS sequence) NM_000101
Accession Version NM_000101.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 588bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytochrome b-245 light chain
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116552.2, BT006861.1, AK300049.1 and BC006465.1. On Jan 12, 2012 this sequence version replaced gi:68509913. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM051065.1, BG749979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGGGGCAGA TCGAGTGGGC CATGTGGGCC AACGAGCAGG CGCTGGCGTC CGGCCTGATC 
CTCATCACCG GGGGCATCGT GGCCACAGCT GGGCGCTTCA CCCAGTGGTA CTTTGGTGCC
TACTCCATTG TGGCGGGCGT GTTTGTGTGC CTGCTGGAGT ACCCCCGGGG GAAGAGGAAG
AAGGGCTCCA CCATGGAGCG CTGGGGACAG AAGTACATGA CCGCCGTGGT GAAGCTGTTC
GGGCCCTTTA CCAGGAATTA CTATGTTCGG GCCGTCCTGC ATCTCCTGCT CTCGGTGCCC
GCCGGCTTCC TGCTGGCCAC CATCCTTGGG ACCGCCTGCC TGGCCATTGC GAGCGGCATC
TACCTACTGG CGGCTGTGCG TGGCGAGCAG TGGACGCCCA TCGAGCCCAA GCCCCGGGAG
CGGCCGCAGA TCGGAGGCAC CATCAAGCAG CCGCCCAGCA ACCCCCCGCC GCGGCCCCCG
GCCGAGGCCC GCAAGAAGCC CAGCGAGGAG GAGGCTGCGG TGGCGGCGGG GGGACCCCCG
GGAGGTCCCC AGGTCAACCC CATCCCGGTG ACCGACGAGG TCGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000092.2
CDS72..659
Misc Feature(1)42..44(+)
Misc Feature(2)75..656(+)
Misc Feature(3)510..512(+)
Exon (1)1..129
Gene:CYBA
Gene Synonym:
Exon (2)130..199
Gene:CYBA
Gene Synonym:
Exon (3)200..274
Gene:CYBA
Gene Synonym:
Exon (4)275..358
Gene:CYBA
Gene Synonym:
Exon (5)359..440
Gene:CYBA
Gene Synonym:
Exon (6)441..723
Gene:CYBA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000101
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome b-245, alpha polypeptide (CYBA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000101

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGGCAGA TCGAGTGGGC CATGTGGGCC AACGAGCAGG CGCTGGCGTC CGGCCTGATC 
CTCATCACCG GGGGCATCGT GGCCACAGCT GGGCGCTTCA CCCAGTGGTA CTTTGGTGCC
TACTCCATTG TGGCGGGCGT GTTTGTGTGC CTGCTGGAGT ACCCCCGGGG GAAGAGGAAG
AAGGGCTCCA CCATGGAGCG CTGGGGACAG AAGTACATGA CCGCCGTGGT GAAGCTGTTC
GGGCCCTTTA CCAGGAATTA CTATGTTCGG GCCGTCCTGC ATCTCCTGCT CTCGGTGCCC
GCCGGCTTCC TGCTGGCCAC CATCCTTGGG ACCGCCTGCC TGGCCATTGC GAGCGGCATC
TACCTACTGG CGGCTGTGCG TGGCGAGCAG TGGACGCCCA TCGAGCCCAA GCCCCGGGAG
CGGCCGCAGA TCGGAGGCAC CATCAAGCAG CCGCCCAGCA ACCCCCCGCC GCGGCCCCCG
GCCGAGGCCC GCAAGAAGCC CAGCGAGGAG GAGGCTGCGG TGGCGGCGGG GGGACCCCCG
GGAGGTCCCC AGGTCAACCC CATCCCGGTG ACCGACGAGG TCGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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